##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-23A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 605553 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.567760377704346 40.0 40.0 40.0 40.0 40.0 2 39.39126715580635 40.0 40.0 40.0 40.0 40.0 3 39.33781518710997 40.0 40.0 40.0 40.0 40.0 4 39.1887679525987 40.0 40.0 40.0 40.0 40.0 5 39.269738569538916 40.0 40.0 40.0 40.0 40.0 6 39.38245537549975 40.0 40.0 40.0 40.0 40.0 7 39.37209790059664 40.0 40.0 40.0 40.0 40.0 8 39.39503891484313 40.0 40.0 40.0 40.0 40.0 9 39.407120433719264 40.0 40.0 40.0 40.0 40.0 10-11 39.4132305512482 40.0 40.0 40.0 40.0 40.0 12-13 39.331989107476964 40.0 40.0 40.0 40.0 40.0 14-15 39.37183698206432 40.0 40.0 40.0 40.0 40.0 16-17 39.46983335892977 40.0 40.0 40.0 40.0 40.0 18-19 39.50377588749457 40.0 40.0 40.0 40.0 40.0 20-21 39.490194912749175 40.0 40.0 40.0 40.0 40.0 22-23 39.53252316477666 40.0 40.0 40.0 40.0 40.0 24-25 39.50500121376659 40.0 40.0 40.0 40.0 40.0 26-27 39.48556113172587 40.0 40.0 40.0 40.0 40.0 28-29 39.50389478707892 40.0 40.0 40.0 40.0 40.0 30-31 39.47586079320509 40.0 40.0 40.0 40.0 40.0 32-33 39.48336760053276 40.0 40.0 40.0 40.0 40.0 34-35 39.47741551888691 40.0 40.0 40.0 40.0 40.0 36-37 39.461179164798786 40.0 40.0 40.0 40.0 40.0 38-39 39.39748274571068 40.0 40.0 40.0 40.0 40.0 40-41 39.44524971722113 40.0 40.0 40.0 40.0 40.0 42-43 39.41905270085708 40.0 40.0 40.0 40.0 40.0 44-45 39.30864005393306 40.0 40.0 40.0 40.0 40.0 46-47 39.30260982312869 40.0 40.0 40.0 40.0 40.0 48-49 39.390624233575736 40.0 40.0 40.0 40.0 40.0 50-51 39.36459594586761 40.0 40.0 40.0 40.0 40.0 52-53 39.3141262527057 40.0 40.0 40.0 40.0 40.0 54-55 39.2569087529688 40.0 40.0 40.0 40.0 40.0 56-57 39.2478332106098 40.0 40.0 40.0 40.0 40.0 58-59 39.187665033110505 40.0 40.0 40.0 40.0 40.0 60-61 39.10938690079589 40.0 40.0 40.0 40.0 40.0 62-63 39.38271267903718 40.0 40.0 40.0 40.0 40.0 64-65 39.38666957568591 40.0 40.0 40.0 40.0 40.0 66-67 39.38647465575037 40.0 40.0 40.0 40.0 40.0 68-69 39.40642409097251 40.0 40.0 40.0 40.0 40.0 70-71 39.39193115842471 40.0 40.0 40.0 40.0 40.0 72-73 39.39904384087409 40.0 40.0 40.0 40.0 40.0 74-75 39.39968254813199 40.0 40.0 40.0 40.0 40.0 76-77 39.39128681629761 40.0 40.0 40.0 40.0 40.0 78-79 39.38788522785944 40.0 40.0 40.0 40.0 40.0 80-81 39.409151418362185 40.0 40.0 40.0 40.0 40.0 82-83 39.398416703172686 40.0 40.0 40.0 40.0 40.0 84-85 39.39032920422415 40.0 40.0 40.0 40.0 40.0 86-87 39.33799799782327 40.0 40.0 40.0 40.0 40.0 88-89 39.41265507556923 40.0 40.0 40.0 40.0 40.0 90-91 39.38000644845718 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.14294336462715762 1101 2 -0.046527301505733476 1101 3 4.904315435325657E-4 1101 4 0.0056323682024554955 1101 5 0.02131334935876339 1101 6 -0.11481538639646516 1101 7 -0.10220174421009887 1101 8 -0.10533309207775687 1101 9 -0.05938152306042355 1101 10-11 -0.026563220875139848 1101 12-13 -0.07301545966411993 1101 14-15 -0.05775940475153618 1101 16-17 0.03848797642073265 1101 18-19 -0.07567322509137142 1101 20-21 -0.05790154560983751 1101 22-23 -0.04401891187470852 1101 24-25 -0.051774111932409994 1101 26-27 -0.0991139302960562 1101 28-29 -0.028801199902190433 1101 30-31 -0.011883262140329975 1101 32-33 0.009287780804854151 1101 34-35 -3.295441185997561E-4 1101 36-37 -0.06217325415707364 1101 38-39 -0.02760554582210517 1101 40-41 -0.035956259605555374 1101 42-43 -0.014070042748599576 1101 44-45 -0.07966456425055668 1101 46-47 -0.04092831394486751 1101 48-49 -0.052359088172160284 1101 50-51 0.033408395765484045 1101 52-53 0.027866133803414073 1101 54-55 -0.10825032326673067 1101 56-57 -0.01327762298920021 1101 58-59 -0.1717672782735633 1101 60-61 -0.09164872431366433 1101 62-63 -0.12422738987269355 1101 64-65 0.06667555249199353 1101 66-67 -0.12354687913253315 1101 68-69 0.15624274414039263 1101 70-71 -0.04164189373237548 1101 72-73 0.07542337787029396 1101 74-75 -0.05091045044913045 1101 76-77 -0.25589850462806396 1101 78-79 0.2195721694970345 1101 80-81 -0.1480955452236259 1101 82-83 -0.4749835216105467 1101 84-85 -0.9147265828448781 1101 86-87 -0.5182793244955377 1101 88-89 -0.6601214068333334 1101 90-91 -2.5353541270820656 1102 1 0.02514947511349419 1102 2 0.042715068779223486 1102 3 -0.04848445356559239 1102 4 -0.10099088582366988 1102 5 0.00939748396056217 1102 6 -0.07261920422165247 1102 7 -0.09922164732303429 1102 8 -0.14660675564109482 1102 9 -0.10085416122623769 1102 10-11 0.01464713258742023 1102 12-13 -0.04294316179010593 1102 14-15 0.008702634625088024 1102 16-17 -0.06437963198367669 1102 18-19 -0.0800664938710085 1102 20-21 -0.13076016624101783 1102 22-23 -0.14422297389599947 1102 24-25 -0.07463358046673108 1102 26-27 -0.10312733847097633 1102 28-29 -0.06411919184145631 1102 30-31 0.005524754501180951 1102 32-33 0.02846339363934902 1102 34-35 -0.11346429063814867 1102 36-37 -0.04520111482246847 1102 38-39 -0.15092448804217895 1102 40-41 -0.1360549290780142 1102 42-43 -0.06713427519378001 1102 44-45 -0.028991633227668956 1102 46-47 -0.09397903458246759 1102 48-49 -0.07796535080488809 1102 50-51 -0.18558702114329861 1102 52-53 -0.0065270728032089664 1102 54-55 -0.0524598499150386 1102 56-57 -0.247355010645542 1102 58-59 -0.25492076715184453 1102 60-61 -0.14595695869664382 1102 62-63 0.06069928852973305 1102 64-65 0.2687197716360785 1102 66-67 -0.11020565008054461 1102 68-69 -0.23207180093122304 1102 70-71 -0.24942925194305587 1102 72-73 -0.33084849287705254 1102 74-75 -0.06876209877761852 1102 76-77 0.04190166649086535 1102 78-79 -0.08729992239516804 1102 80-81 -0.2055297455149656 1102 82-83 -0.12700264360909586 1102 84-85 -0.6451524436273175 1102 86-87 -0.014964752888275257 1102 88-89 -1.2566770251280772 1102 90-91 0.7373731456452077 1103 1 0.01963384787097766 1103 2 -0.05873449458535873 1103 3 -0.0032642590392697457 1103 4 -0.20650320104022057 1103 5 0.06444335552019709 1103 6 0.015882545632067036 1103 7 0.013824422958158777 1103 8 0.11108211263962886 1103 9 0.06975492034896291 1103 10-11 -0.014540648244782517 1103 12-13 0.02192838680284126 1103 14-15 -0.13828728577589544 1103 16-17 -0.006706219824131665 1103 18-19 -0.0707663249576882 1103 20-21 0.018119816978050096 1103 22-23 0.10763010463213618 1103 24-25 -0.03761617025022446 1103 26-27 -0.02921395899007706 1103 28-29 -0.027399863961925064 1103 30-31 -0.05941524262838982 1103 32-33 0.024685469053984832 1103 34-35 0.06968818310313907 1103 36-37 0.10645174926266776 1103 38-39 0.08053862085665742 1103 40-41 0.12989664902922016 1103 42-43 0.022719487761882817 1103 44-45 0.09772285688024596 1103 46-47 -0.01450237831656409 1103 48-49 0.035389486851812535 1103 50-51 0.10059133785593843 1103 52-53 -0.1578941808940968 1103 54-55 -0.19900841607500297 1103 56-57 0.08859514828978376 1103 58-59 -0.1397162722177825 1103 60-61 -0.01676679926258373 1103 62-63 -0.09370434240369008 1103 64-65 0.07091422897821786 1103 66-67 -0.02535616201393509 1103 68-69 -0.20643630003280578 1103 70-71 -0.04356495397894378 1103 72-73 0.14038083920340938 1103 74-75 0.03633581522834817 1103 76-77 0.14749223218093022 1103 78-79 -0.1725097137140139 1103 80-81 -0.18504322923013206 1103 82-83 0.13161281500637045 1103 84-85 -0.07642520099242489 1103 86-87 -0.030636038124782772 1103 88-89 0.011097269542766242 1103 90-91 0.26678491035109175 1104 1 0.08545035826821845 1104 2 -0.0013816122942742481 1104 3 -0.004503455584689675 1104 4 -0.15517770330681202 1104 5 0.03525479743496618 1104 6 0.11602564332838483 1104 7 0.0066969353448556035 1104 8 -0.02834435853745987 1104 9 -0.05853985669049422 1104 10-11 -0.10400515938385979 1104 12-13 -0.028204278881744926 1104 14-15 -0.19611856537277816 1104 16-17 -0.05398957644508329 1104 18-19 -0.07735008347452776 1104 20-21 -0.033633498610321055 1104 22-23 -0.04387431790859608 1104 24-25 -0.021601451630040458 1104 26-27 -0.13543291321778383 1104 28-29 -0.0165927213004764 1104 30-31 -0.05130777815518428 1104 32-33 -0.008840129016519427 1104 34-35 -0.007031701444759619 1104 36-37 -0.1761965071913849 1104 38-39 -0.03576984839833841 1104 40-41 -0.13472765795401642 1104 42-43 -0.009804691706243318 1104 44-45 -0.14925505662270666 1104 46-47 -0.0726115467534072 1104 48-49 -0.028645269841355514 1104 50-51 0.1778160859663842 1104 52-53 0.022715612172689248 1104 54-55 -0.06498630614225931 1104 56-57 0.012544692906665489 1104 58-59 0.17813053879041263 1104 60-61 -0.23792583907698628 1104 62-63 0.020969171010136733 1104 64-65 -0.2278228272195193 1104 66-67 0.0032228771019617852 1104 68-69 0.14743201547267404 1104 70-71 0.15890063175645253 1104 72-73 0.19290704953084514 1104 74-75 0.3345590103053908 1104 76-77 0.042435224702607854 1104 78-79 -0.2839381624149482 1104 80-81 -0.44874625129783396 1104 82-83 0.16157836916546842 1104 84-85 0.31231956326329424 1104 86-87 -0.17735791528143352 1104 88-89 0.6069593385082825 1104 90-91 0.7373731456452077 1201 1 -0.020183102773721373 1201 2 0.04267439091161407 1201 3 -0.004087466633357906 1201 4 0.18921954950215536 1201 5 -0.08599211558036046 1201 6 -0.025918106645619332 1201 7 -0.03266393923031785 1201 8 0.0011381650733781612 1201 9 0.022354741415647084 1201 10-11 0.08953508685386424 1201 12-13 -0.1070979701319672 1201 14-15 0.027780554533265445 1201 16-17 0.06790697264924006 1201 18-19 0.1016742283064147 1201 20-21 -0.024081005657869525 1201 22-23 -0.01827921104796104 1201 24-25 -0.024650650643316396 1201 26-27 0.06585651386836133 1201 28-29 0.05540109987696695 1201 30-31 -0.0945144705854375 1201 32-33 -0.11909597270112471 1201 34-35 -0.018997737150044713 1201 36-37 -0.08068373217974312 1201 38-39 0.16763185422823312 1201 40-41 0.05464074446848599 1201 42-43 0.10311908881080711 1201 44-45 0.08405400691409426 1201 46-47 -3.309425292812307E-4 1201 48-49 0.014127949548004892 1201 50-51 -0.0034738738650830214 1201 52-53 -0.0705451731904958 1201 54-55 -0.0035223807508089067 1201 56-57 -0.029543672864086545 1201 58-59 -0.17886337464798885 1201 60-61 -0.17450645797784858 1201 62-63 -0.33576258365087597 1201 64-65 -0.053701599052054405 1201 66-67 -0.07845996636896757 1201 68-69 -0.32389484345087993 1201 70-71 -0.27920752278807726 1201 72-73 -0.276147592138571 1201 74-75 -0.27341654741504584 1201 76-77 -0.41373341113306594 1201 78-79 -0.33198134068363316 1201 80-81 0.12599595518366158 1201 82-83 -0.4124322871782695 1201 84-85 0.309484329114575 1201 86-87 0.11893838101485699 1201 88-89 0.3310972695427665 1201 90-91 0.7373731456452077 1202 1 0.07322380557951647 1202 2 0.09479391350349431 1202 3 -0.07355406637974937 1202 4 0.08876923570851147 1202 5 -0.06465219087964869 1202 6 0.08821078571666163 1202 7 -0.12327753196825597 1202 8 0.1100551051764711 1202 9 -0.08770161421621481 1202 10-11 0.017423365413698377 1202 12-13 0.0518022632035553 1202 14-15 0.09461321703279424 1202 16-17 -0.10347440440782663 1202 18-19 0.015659540331519395 1202 20-21 -0.0275607667021589 1202 22-23 0.03193965459747261 1202 24-25 0.021184610842063023 1202 26-27 0.09106136400377096 1202 28-29 0.06985420139399423 1202 30-31 0.0604691092484444 1202 32-33 -0.045742254045258335 1202 34-35 0.08420944162240573 1202 36-37 -0.13955163321165998 1202 38-39 -0.2104786486189809 1202 40-41 -0.06314603638220717 1202 42-43 -0.11451796336557862 1202 44-45 0.008374203302864203 1202 46-47 -0.05723423917784487 1202 48-49 -0.033117490336323385 1202 50-51 -0.04685208313356526 1202 52-53 -0.011967493822851338 1202 54-55 -0.04260867211435482 1202 56-57 -0.007226546982842308 1202 58-59 0.13761780277449986 1202 60-61 -0.021064190674195515 1202 62-63 0.034814521084797434 1202 64-65 -0.46525401194400473 1202 66-67 -0.23873021341478307 1202 68-69 -0.32609055845028934 1202 70-71 -0.29849730470608904 1202 72-73 -0.093404236372713 1202 74-75 -0.21431726732128453 1202 76-77 -0.003521095424495968 1202 78-79 -0.24860359903465223 1202 80-81 -0.10882632510334389 1202 82-83 -0.003202475505553082 1202 84-85 -0.006787663753094364 1202 86-87 -0.03854117646470456 1202 88-89 -1.3097073281583889 1202 90-91 0.7373731456452077 1203 1 0.004588243934300351 1203 2 -0.0013910825101106639 1203 3 -0.15939830574896519 1203 4 -0.03433910216592295 1203 5 -0.01280654791742819 1203 6 0.052722662036792656 1203 7 0.09951046205999603 1203 8 0.09229672095891317 1203 9 0.03002560177956326 1203 10-11 0.013608156821909745 1203 12-13 -0.07261350314253434 1203 14-15 -0.08535018588228382 1203 16-17 -0.058915441566213644 1203 18-19 0.0034186815890322464 1203 20-21 0.03191324738968859 1203 22-23 -0.06318002932811595 1203 24-25 0.04490178616911322 1203 26-27 -0.06675964310490912 1203 28-29 -0.02550487084330655 1203 30-31 -0.016827398751289024 1203 32-33 0.033174319430322896 1203 34-35 -0.019844142358977024 1203 36-37 -0.055664552593178485 1203 38-39 0.04370119058638977 1203 40-41 -0.049951303908294165 1203 42-43 -0.04917988612081814 1203 44-45 -0.08658371388789021 1203 46-47 -0.08054106935211536 1203 48-49 -0.08705496693060866 1203 50-51 -0.14153632254521398 1203 52-53 0.10172898000958952 1203 54-55 -0.12507972863821237 1203 56-57 0.06218544333007259 1203 58-59 -0.1618461925871486 1203 60-61 0.07637960544514755 1203 62-63 -0.1919315257768659 1203 64-65 -0.1228774729557216 1203 66-67 -0.18433451995507255 1203 68-69 -0.14359297950281302 1203 70-71 -0.029037987889481087 1203 72-73 0.13878189007601094 1203 74-75 0.0357973239697742 1203 76-77 0.01002222315875656 1203 78-79 0.1957889492749203 1203 80-81 0.22432511140755196 1203 82-83 -0.07582839243483619 1203 84-85 0.003657080182215111 1203 86-87 -0.25311549103901143 1203 88-89 -0.756003624454685 1203 90-91 0.7373731456452077 1204 1 -0.052793949962953945 1204 2 -0.08517101684768846 1204 3 -0.2973376645747692 1204 4 -0.2208600669313796 1204 5 -0.08370479262795527 1204 6 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0.014625964103707645 2401 38-39 -0.007480043279016968 2401 40-41 -0.02155396188834402 2401 42-43 0.17828395964819066 2401 44-45 0.15303394029496076 2401 46-47 0.053267677508834765 2401 48-49 0.12337109647827305 2401 50-51 0.03814881270195514 2401 52-53 -0.020705709976596154 2401 54-55 0.1146611445509933 2401 56-57 -0.03526520369515396 2401 58-59 0.12393809913562848 2401 60-61 0.018056926426154973 2401 62-63 0.13111847350769068 2401 64-65 0.2147628353261979 2401 66-67 -0.08757811834456675 2401 68-69 0.021674197642362003 2401 70-71 -0.255095721593392 2401 72-73 0.12700324763198978 2401 74-75 0.16812655959414968 2401 76-77 0.3171793527284805 2401 78-79 0.10546730848751196 2401 80-81 0.11826130936852053 2401 82-83 0.18263898182200222 2401 84-85 -0.09727662663384962 2401 86-87 0.08978156462399767 2401 88-89 -0.30329707174812626 2401 90-91 -0.5515157432436837 2402 1 0.06827396266587016 2402 2 0.10328556675483469 2402 3 0.11830992810327956 2402 4 0.02705264735188706 2402 5 0.13673992527869672 2402 6 0.01973976948599443 2402 7 0.009574035486522803 2402 8 0.12453741554498521 2402 9 0.04000998246937115 2402 10-11 0.09919192386158926 2402 12-13 0.10216930502869559 2402 14-15 0.05337720127608492 2402 16-17 0.02900614211741015 2402 18-19 0.08539626485770668 2402 20-21 0.019495334324176383 2402 22-23 0.031450211175034326 2402 24-25 0.060050003851280565 2402 26-27 -0.0404185667788326 2402 28-29 0.05036989875716813 2402 30-31 0.05777472592407662 2402 32-33 0.044155438893319854 2402 34-35 0.024601719680873657 2402 36-37 0.07168147975658457 2402 38-39 -0.4313857478127119 2402 40-41 0.0857693955495904 2402 42-43 0.039956398757176714 2402 44-45 0.14081648690630288 2402 46-47 0.16462794474889364 2402 48-49 0.08534118791414613 2402 50-51 0.15567279633429365 2402 52-53 0.06791334477074429 2402 54-55 0.022137530731122013 2402 56-57 0.09123600278811494 2402 58-59 0.09470427696506079 2402 60-61 0.03134631920454467 2402 62-63 0.11888774182375528 2402 64-65 0.15813929616198408 2402 66-67 -0.0059103650431069354 2402 68-69 -0.09135398299821418 2402 70-71 0.15254632817759273 2402 72-73 0.001239537657752976 2402 74-75 -5.19885840979839E-4 2402 76-77 -0.023652109413653477 2402 78-79 0.046304713844740775 2402 80-81 -0.0572210478975137 2402 82-83 0.33857737148548495 2402 84-85 0.4305721940538447 2402 86-87 0.2990595555552886 2402 88-89 0.08209161364055717 2402 90-91 0.7373731456452077 2403 1 -0.1388249207338177 2403 2 -0.006972660040176493 2403 3 0.12669307902662297 2403 4 0.17365547314299334 2403 5 0.009619790474005185 2403 6 -0.00711214390572934 2403 7 0.035625037600553355 2403 8 0.12831637493480486 2403 9 0.030846886532145845 2403 10-11 -0.015014669734853214 2403 12-13 0.13670828938514035 2403 14-15 0.09734513379491005 2403 16-17 -0.00698286904439982 2403 18-19 0.021715431775795935 2403 20-21 0.012612529239355297 2403 22-23 -0.007211579297766946 2403 24-25 0.03777292922375608 2403 26-27 0.00914022964860095 2403 28-29 -0.032760079289062105 2403 30-31 0.03321358651665918 2403 32-33 0.04899651936720062 2403 34-35 -0.05760595705825722 2403 36-37 0.04806835219695671 2403 38-39 0.039889230704652334 2403 40-41 0.03041496487806228 2403 42-43 -0.0035943052759463967 2403 44-45 0.022178954854410904 2403 46-47 -0.07501533708795449 2403 48-49 0.06195488618740086 2403 50-51 0.019417444871059786 2403 52-53 -0.06691898352417525 2403 54-55 0.07342349913182744 2403 56-57 -0.056438320280499 2403 58-59 0.022771918320088957 2403 60-61 -0.1641488155837152 2403 62-63 -0.021950601667121816 2403 64-65 -0.07121382687811462 2403 66-67 0.0774183890635669 2403 68-69 0.10770790568265909 2403 70-71 -0.178853604371767 2403 72-73 -0.17484117374086594 2403 74-75 -0.5814958094998346 2403 76-77 0.05193791739713305 2403 78-79 -0.5085090426316228 2403 80-81 0.11094607558270297 2403 82-83 0.14779126682838495 2403 84-85 0.11805893848650584 2403 86-87 -0.33410394702747226 2403 88-89 -0.004151772602831727 2403 90-91 0.7373731456452077 2404 1 -0.03190423413074939 2404 2 -0.053018896865729914 2404 3 0.07822616041395491 2404 4 0.19810575274485842 2404 5 0.1321023541670172 2404 6 0.08772972585577321 2404 7 -0.026790516734962466 2404 8 0.07582127132318561 2404 9 0.1361600699233847 2404 10-11 0.03354364949388611 2404 12-13 0.04147540532886751 2404 14-15 0.0761778987434738 2404 16-17 0.06810786041244654 2404 18-19 -0.024504179312536678 2404 20-21 0.06946412230495724 2404 22-23 -0.008279286046068535 2404 24-25 0.03299181627985348 2404 26-27 -0.03046943643789035 2404 28-29 0.03598302083540261 2404 30-31 0.03677452172939866 2404 32-33 -0.01606033133146667 2404 34-35 0.03169548721298554 2404 36-37 0.023233252991438746 2404 38-39 0.07348884911544928 2404 40-41 0.11650638520585233 2404 42-43 0.07779663359338684 2404 44-45 0.047817296077539595 2404 46-47 0.08036536517094106 2404 48-49 -0.014007468527687195 2404 50-51 -0.03268053990195341 2404 52-53 0.07017509877172756 2404 54-55 0.16259370055914246 2404 56-57 0.14474546234183805 2404 58-59 0.10411587938735067 2404 60-61 0.12192271671219146 2404 62-63 0.14831776226204596 2404 64-65 0.2212227690434716 2404 66-67 0.0720055869294498 2404 68-69 0.13443534920764222 2404 70-71 0.20609838289045967 2404 72-73 -0.03058006429112936 2404 74-75 0.2278943306799519 2404 76-77 -0.035675808737373416 2404 78-79 0.29680856304190684 2404 80-81 0.1627135825854964 2404 82-83 0.5026213444307359 2404 84-85 0.20410384927886582 2404 86-87 0.45756271963919914 2404 88-89 0.6069593385082825 2404 90-91 0.7373731456452077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 2.0 30 28.0 31 386.0 32 2288.0 33 4295.0 34 5833.0 35 7363.0 36 10493.0 37 15562.0 38 39819.0 39 238740.0 40 280744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.61485617278752 10.234281722656812 34.65840314555456 30.492458959001112 2 26.724891118183635 16.41328349901647 30.23867290531535 26.623152477484545 3 28.49973495300989 18.69101465932792 21.77431207507848 31.034938312583705 4 26.80822322736408 19.745422778848422 20.68439921856551 32.76195477522199 5 27.33006029199756 19.89338670603564 21.124162542337334 31.652390459629466 6 28.78212146583371 21.917486991229502 22.960170290626913 26.34022125230987 7 26.48768976456231 21.411007789574157 22.656315797296024 29.444986648567507 8 25.497025033316657 22.275011435828077 23.069326714589806 29.158636816265464 9 26.333450581534564 22.286240841016394 23.024409093836542 28.3558994836125 10-11 25.556805102113273 21.286245795165744 23.044968813629858 30.11198028909113 12-13 26.27350537442635 21.190465574441873 22.727490409592555 29.808538641539222 14-15 26.54028631680464 20.90642767850213 22.63187532718028 29.92141067751295 16-17 26.45036850614232 20.662518392279452 22.368727427657035 30.518385673921195 18-19 26.820608600733543 20.935987436277255 22.211515755020617 30.03188820796858 20-21 26.827296702353053 21.081969703725356 22.216304766056812 29.87442882786478 22-23 26.323955128617975 20.674325781558345 22.51850787627177 30.483211213551908 24-25 26.688910797238226 21.02227220408453 22.402993627312558 29.88582337136469 26-27 26.826058165016107 21.13464882512348 22.446094726638297 29.59319828322211 28-29 26.465396092497272 20.85919812138657 22.610902761607985 30.064503024508177 30-31 26.914079949400993 21.109809254630743 22.542071570179285 29.43403922578898 32-33 26.88843668315609 21.11401544500166 22.71675365477782 29.280794217064425 34-35 26.550163578826442 20.7148412554006 23.016450642720297 29.718544523052664 36-37 26.806773766802223 21.033754043541343 22.997611981866868 29.16186020778957 38-39 27.06593170448923 20.999995002523725 22.993388338888863 28.940684954098177 40-41 26.696387784812558 20.693924425678706 23.215345403935398 29.394342385573342 42-43 26.842946509769337 20.973649883796767 23.29449940750131 28.888904198932586 44-45 27.02769532041158 20.92948124548797 23.375948846473566 28.66687458762688 46-47 26.55983662134674 20.766485765148587 23.553350640088823 29.12032697341585 48-49 26.846831682583556 21.079100149423862 23.41185468677097 28.662213481221617 50-51 27.01289002889704 20.89337316146628 23.528361310432157 28.56537549920452 52-53 26.76904271583298 20.713047748605145 23.666037570146052 28.85187196541582 54-55 26.92411848571497 21.071715876416754 23.636410701627227 28.367754936241052 56-57 27.085918631493268 20.91189303870942 23.57413856753355 28.42804976226376 58-59 26.828397610482725 20.87607312342261 23.671998062823434 28.62353120327123 60-61 27.171701229376517 21.119234303043285 23.641400846640774 28.06766362093942 62-63 27.308233638282896 20.90877103192374 23.506493506493506 28.276501823299853 64-65 26.849574405099098 21.006735692592198 23.70247462694204 28.44121527536666 66-67 27.121224867502313 21.176915958610245 23.439892319340455 28.261966854546984 68-69 27.237501640842638 21.057723628620185 23.540136226402254 28.164638504134924 70-71 27.093216352393746 20.62355828359943 23.69714178838012 28.586083575626702 72-73 27.14314381270903 21.047491638795986 23.71304347826087 28.096321070234115 74-75 27.27620901786498 21.027854686465222 23.3896384715429 28.306297824126897 76-77 27.333788816289612 20.877799055033247 23.446145555673166 28.342266573003975 78-79 27.295449186512617 21.323980193350625 23.342372082056116 28.03819853808064 80-81 27.723255195246683 21.010080108782407 23.111827130568447 28.154837565402463 82-83 27.27168732125834 20.3813155386082 23.437559580552907 28.909437559580553 84-85 28.306395662563915 20.746717372870467 22.727856720713312 28.2190302438523 86-87 28.328294703840555 19.587205613406486 22.67383271750084 29.41066696525212 88-89 28.825444527923867 18.582519408965688 22.61457550713749 29.97746055597295 90-91 25.085271317829456 18.790697674418606 26.046511627906977 30.077519379844965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 1.0 9 2.0 10 5.0 11 10.0 12 12.0 13 13.0 14 18.0 15 28.5 16 43.5 17 63.5 18 91.5 19 140.0 20 209.5 21 275.0 22 347.5 23 478.5 24 661.5 25 832.5 26 1031.0 27 1306.5 28 1639.5 29 2064.0 30 2577.0 31 3107.5 32 3661.5 33 4294.5 34 5175.5 35 6178.0 36 7152.0 37 8204.0 38 9495.0 39 11011.0 40 12512.5 41 14129.0 42 15996.0 43 17935.0 44 20004.5 45 22192.0 46 24692.5 47 27601.5 48 30169.5 49 32535.0 50 35264.5 51 38106.5 52 40401.0 53 42464.5 54 44305.0 55 45699.5 56 46726.5 57 47352.0 58 47765.5 59 47758.0 60 47411.0 61 46623.5 62 45403.0 63 43802.0 64 41606.5 65 38883.5 66 35826.5 67 32911.0 68 30041.0 69 26733.0 70 23178.0 71 19851.0 72 16881.5 73 13987.5 74 11199.5 75 9039.5 76 7249.0 77 5516.5 78 4241.5 79 3297.0 80 2488.5 81 1864.0 82 1374.5 83 971.5 84 698.5 85 520.5 86 366.5 87 246.5 88 170.0 89 110.5 90 81.0 91 64.0 92 44.5 93 31.0 94 26.0 95 19.5 96 11.5 97 7.0 98 5.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01321106492742997 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06262910385443678 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.309433920889997E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.688918468772784E-4 74-75 0.0 76-77 9.62278675904542E-4 78-79 0.0 80-81 0.0 82-83 0.0019065413433490305 84-85 0.0 86-87 0.0 88-89 0.006260564702936205 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11972.0 32-33 25957.0 34-35 28841.0 36-37 30217.0 38-39 32000.0 40-41 32846.0 42-43 33073.0 44-45 32550.0 46-47 31771.0 48-49 30766.0 50-51 29490.0 52-53 28284.0 54-55 26486.0 56-57 25001.0 58-59 23493.0 60-61 21330.0 62-63 20020.0 64-65 18391.0 66-67 16332.0 68-69 15040.0 70-71 13802.0 72-73 12365.0 74-75 10999.0 76-77 9905.0 78-79 8788.0 80-81 7798.0 82-83 6978.0 84-85 6214.0 86-87 5587.0 88-89 4926.0 90-91 4331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.9307201860167 #Duplication Level Percentage of deduplicated Percentage of total 1 99.93511928095504 99.8658844162132 2 0.061076314697477055 0.12206800228053358 3 0.003161683555210717 0.00947847944017478 4 6.42720792290137E-4 0.0025691020660832255 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016513831159287462 0.0 0.0 0.0 0.0 2 0.0016513831159287462 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 3 0.0016513831159287462 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 4 0.0016513831159287462 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 5 0.0018165214275216207 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 6 0.0018165214275216207 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 7 0.0019816597391144955 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 8 0.005119287659379113 1.651383115928746E-4 0.0 0.0 3.302766231857492E-4 9 0.005119287659379113 3.302766231857492E-4 0.0 0.0 3.302766231857492E-4 10-11 0.005119287659379113 3.302766231857492E-4 0.0 0.0 3.302766231857492E-4 12-13 0.005119287659379113 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 14-15 0.005119287659379113 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 16-17 0.005284425970971987 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 18-19 0.005284425970971987 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 20-21 0.005614702594157737 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 22-23 0.005862410061547049 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 24-25 0.006110117528936361 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 26-27 0.006275255840529235 3.302766231857492E-4 0.0 1.651383115928746E-4 3.302766231857492E-4 28-29 0.006357824996325673 3.302766231857492E-4 0.0 1.651383115928746E-4 4.1284577898218654E-4 30-31 0.006605532463714984 3.302766231857492E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 32-33 0.007183516554290045 3.302766231857492E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 34-35 0.007596362333272232 3.302766231857492E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 36-37 0.008009208112254418 3.302766231857492E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 38-39 0.00858719220282948 4.954149347786239E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 40-41 0.009082607137608105 4.954149347786239E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 42-43 0.009330314604997416 4.954149347786239E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 44-45 0.009412883760793854 4.954149347786239E-4 0.0 1.651383115928746E-4 6.605532463714984E-4 46-47 0.009908298695572477 4.954149347786239E-4 0.0 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