##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-22C_S6_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 734465 Sequences flagged as poor quality 0 Sequence length 61 %GC 56 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.283151681836436 40.0 40.0 40.0 40.0 40.0 2 39.245633216014376 40.0 40.0 40.0 40.0 40.0 3 39.15393109270013 40.0 40.0 40.0 40.0 40.0 4 39.04456713390019 40.0 40.0 40.0 40.0 40.0 5 39.046620329083076 40.0 40.0 40.0 40.0 40.0 6 39.200697106056786 40.0 40.0 40.0 40.0 40.0 7 39.202597809289756 40.0 40.0 40.0 40.0 40.0 8 39.196547146562466 40.0 40.0 40.0 40.0 40.0 9 39.226533599286554 40.0 40.0 40.0 40.0 40.0 10 39.24223482398753 40.0 40.0 40.0 40.0 40.0 11 39.22033044460934 40.0 40.0 40.0 40.0 40.0 12 39.229921099031266 40.0 40.0 40.0 40.0 40.0 13 39.120023418406596 40.0 40.0 40.0 40.0 40.0 14 39.24483807941835 40.0 40.0 40.0 40.0 40.0 15 39.20438958970135 40.0 40.0 40.0 40.0 40.0 16 39.21445950453732 40.0 40.0 40.0 40.0 40.0 17 39.24611247642842 40.0 40.0 40.0 40.0 40.0 18 39.195555948887964 40.0 40.0 40.0 40.0 40.0 19 39.248775639411 40.0 40.0 40.0 40.0 40.0 20 38.836077961509396 40.0 40.0 40.0 40.0 40.0 21 39.09454092434629 40.0 40.0 40.0 40.0 40.0 22 39.07437386396901 40.0 40.0 40.0 40.0 40.0 23 39.21396390570007 40.0 40.0 40.0 40.0 40.0 24 39.235078594623296 40.0 40.0 40.0 40.0 40.0 25 38.93329157958514 40.0 40.0 40.0 40.0 40.0 26 39.174207075898785 40.0 40.0 40.0 40.0 40.0 27 39.18180988883064 40.0 40.0 40.0 40.0 40.0 28 38.84912691550993 40.0 40.0 40.0 40.0 40.0 29 39.12158101475224 40.0 40.0 40.0 40.0 40.0 30 38.67791113259311 40.0 40.0 40.0 40.0 40.0 31 38.93746876978481 40.0 40.0 40.0 40.0 40.0 32 38.96313643264145 40.0 40.0 40.0 40.0 40.0 33 39.0147631269019 40.0 40.0 40.0 40.0 40.0 34 38.94790357607238 40.0 40.0 40.0 40.0 40.0 35 38.74026944782937 40.0 40.0 40.0 40.0 40.0 36 38.84777082638383 40.0 40.0 40.0 40.0 40.0 37 38.81206592553764 40.0 40.0 40.0 40.0 40.0 38 38.573236301253296 40.0 40.0 40.0 40.0 40.0 39 38.82749484318518 40.0 40.0 40.0 40.0 40.0 40 38.31362964879198 40.0 40.0 40.0 40.0 40.0 41 38.51145527697031 40.0 40.0 40.0 40.0 40.0 42 38.133030164813846 40.0 40.0 40.0 40.0 40.0 43 38.42489158775435 40.0 40.0 40.0 40.0 40.0 44 37.91892057484019 40.0 40.0 40.0 24.0 40.0 45 38.19948942427481 40.0 40.0 40.0 40.0 40.0 46 37.73867781310205 40.0 40.0 40.0 24.0 40.0 47 37.19731232938261 40.0 40.0 40.0 24.0 40.0 48 37.448896816049775 40.0 40.0 40.0 24.0 40.0 49 37.13217648220133 40.0 40.0 40.0 24.0 40.0 50 37.30033153383756 40.0 40.0 40.0 24.0 40.0 51 36.827769873309144 40.0 40.0 40.0 24.0 40.0 52 36.77970767837814 40.0 40.0 40.0 24.0 40.0 53 35.999948261659846 40.0 40.0 40.0 24.0 40.0 54 35.26453677166373 40.0 40.0 40.0 12.0 40.0 55 35.04808534103054 40.0 40.0 40.0 12.0 40.0 56 34.80501317285371 40.0 40.0 40.0 12.0 40.0 57 34.834011150973836 40.0 40.0 40.0 12.0 40.0 58 34.76374231583534 40.0 40.0 40.0 12.0 40.0 59 34.27628001334305 40.0 24.0 40.0 12.0 40.0 60 33.50758170913522 40.0 24.0 40.0 12.0 40.0 61 33.43108248861416 40.0 24.0 40.0 12.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13427780504820674 1101 2 0.12729740401512402 1101 3 -0.010245706790556142 1101 4 0.100697993808474 1101 5 0.1854930352320494 1101 6 0.058035143715251536 1101 7 0.11301363870878589 1101 8 0.044035183489441465 1101 9 0.09083885709705442 1101 10 0.061764809644287766 1101 11 0.03727993137079011 1101 12 0.08695928867240355 1101 13 0.10503412137607882 1101 14 0.11816765201160706 1101 15 0.1952916223930501 1101 16 0.08536169269702754 1101 17 0.07517381120354116 1101 18 0.1769063613202846 1101 19 0.08143965040732581 1101 20 0.232773426770585 1101 21 0.054807488361149126 1101 22 0.12087165085151952 1101 23 0.08296158942561505 1101 24 0.04561468176331829 1101 25 0.11491511662636356 1101 26 0.1256394589999843 1101 27 0.04235949983026188 1101 28 0.24168390048052402 1101 29 0.08456273712347695 1101 30 0.07545547575107037 1101 31 0.013317482247664714 1101 32 0.20148696714613834 1101 33 0.08288339092221975 1101 34 0.13515520899575506 1101 35 0.19591392887853232 1101 36 0.1172855788136502 1101 37 0.14330905740851563 1101 38 0.19762550507236654 1101 39 0.026732597035959316 1101 40 -0.15673777875517914 1101 41 0.1600180817108452 1101 42 0.18469975908918457 1101 43 0.35065976652750663 1101 44 0.20305747874866853 1101 45 0.2510933090962979 1101 46 0.03694706879663556 1101 47 0.07695959093304339 1101 48 0.3499302560754174 1101 49 0.20153176959571795 1101 50 0.12226083091913864 1101 51 0.23240133548286934 1101 52 0.41971268109431037 1101 53 0.35006911102892957 1101 54 0.3412815082615097 1101 55 0.17847714069989706 1101 56 0.44177415181336954 1101 57 0.2294654109041545 1101 58 0.5357250364589348 1101 59 0.31532245237532663 1101 60 0.42967329152753564 1101 61 0.3043499897672959 1102 1 -0.05848686837452988 1102 2 -0.07035111119770221 1102 3 0.019594559247806842 1102 4 -0.11829899019477352 1102 5 0.04472656914522588 1102 6 -0.06551123635873068 1102 7 0.046832509563984104 1102 8 -0.022886076514005538 1102 9 -0.021828913196692667 1102 10 -0.05605759982471881 1102 11 -0.04296952338487614 1102 12 -0.02740771696543476 1102 13 -0.02404645215964507 1102 14 -0.012831130495023046 1102 15 -0.021181325605091672 1102 16 -0.04616846581068046 1102 17 0.03837310348077949 1102 18 -0.012321888029362071 1102 19 -0.06889596317137858 1102 20 0.12634348519274852 1102 21 -0.11949697840887552 1102 22 0.017431429191816505 1102 23 -0.026326687894261624 1102 24 -0.01932267718117231 1102 25 -0.1224406162491718 1102 26 -0.031682873098333175 1102 27 -0.016236382881622546 1102 28 0.07675880405913205 1102 29 -0.018725662821637457 1102 30 0.18701231984476863 1102 31 -0.015877541503670045 1102 32 -0.05531070414232886 1102 33 -0.04153085462604622 1102 34 0.1573445365131505 1102 35 0.13588595864726472 1102 36 0.14360474893280184 1102 37 0.0076038017495037025 1102 38 -0.015351164017609165 1102 39 -0.10125456818985867 1102 40 0.14040797301047547 1102 41 0.16411580818066795 1102 42 -0.13118721548359957 1102 43 -0.034498784976555896 1102 44 0.018799930727752212 1102 45 -0.09454488223993707 1102 46 -0.034760954689012635 1102 47 -0.14616723567196033 1102 48 0.029802492263293345 1102 49 0.10806798637376858 1102 50 -0.1951312223819599 1102 51 -0.09953202646301662 1102 52 0.1856503115660928 1102 53 -0.12876343837881876 1102 54 -0.21504726750073644 1102 55 0.03589757298303198 1102 56 -0.22106339603237046 1102 57 0.036137975260501776 1102 58 -0.20248615338535814 1102 59 -0.3997417652902939 1102 60 -0.3202646659448689 1102 61 -0.4152002675997082 1103 1 0.08193433421189411 1103 2 0.07425097787718471 1103 3 0.1555464108202358 1103 4 0.07227694772174686 1103 5 0.08239633791502143 1103 6 0.1271749305098595 1103 7 0.08824196202872514 1103 8 0.08725559655065496 1103 9 0.10211393828253534 1103 10 0.17635457012378453 1103 11 0.11888126501085594 1103 12 -0.013667190066492196 1103 13 0.01186072439396213 1103 14 0.12323536723498307 1103 15 0.14922354545126382 1103 16 0.11562315037468807 1103 17 0.09881973532460364 1103 18 0.08232997966749167 1103 19 0.10320493136450892 1103 20 -0.006718543556068823 1103 21 0.1314457088674743 1103 22 0.058533925815304144 1103 23 0.121719223823753 1103 24 0.16147283401495116 1103 25 0.10575109579518482 1103 26 0.1369372161629414 1103 27 0.06976524731277323 1103 28 0.07269959042822194 1103 29 0.03101597651486543 1103 30 0.14153107965441336 1103 31 0.11088782440987899 1103 32 0.10935520216143146 1103 33 0.19472935812763126 1103 34 0.17603999637737644 1103 35 0.14895709991647976 1103 36 0.14661439548239485 1103 37 -0.001887420792741068 1103 38 0.11930999371627138 1103 39 0.1732179485373848 1103 40 -0.002421168711400412 1103 41 0.1905443362867274 1103 42 0.3070725002235193 1103 43 0.17548637759169594 1103 44 0.2399213032863443 1103 45 0.15618922299488247 1103 46 0.11345581678642702 1103 47 0.13155383540421894 1103 48 0.11219091936239067 1103 49 0.21403062208153045 1103 50 0.02077485948575486 1103 51 0.027709675743956552 1103 52 0.1687803143915687 1103 53 0.23774109643145636 1103 54 -0.031199949555137607 1103 55 0.0356631030994734 1103 56 0.18918863320461554 1103 57 0.4605979918583074 1103 58 0.08194800495651577 1103 59 0.23967831746203672 1103 60 0.10241870503678285 1103 61 0.4134843489569704 1104 1 0.05761188366020065 1104 2 -0.08953432696909402 1104 3 0.03278537132955961 1104 4 0.057593938002007405 1104 5 0.07093094606690897 1104 6 0.11017984701981476 1104 7 -0.023999105556292477 1104 8 -0.0283915928822438 1104 9 -0.03430928939491906 1104 10 0.030932452625997087 1104 11 -0.013582654149935536 1104 12 0.024111906836338903 1104 13 0.01937405787833768 1104 14 0.012779287302045361 1104 15 0.04576821319034963 1104 16 -0.026342819219976832 1104 17 -0.06871099852681084 1104 18 0.07810459053818875 1104 19 0.03366001440141986 1104 20 -0.015143221264118267 1104 21 -0.05568001331572958 1104 22 -0.0067885020796580875 1104 23 0.05385851738697767 1104 24 0.1531880842585096 1104 25 0.10474259952221843 1104 26 -0.046681607046608065 1104 27 -0.06522535014514119 1104 28 -0.07690949038832429 1104 29 -0.10553340382362819 1104 30 -0.031456119294318796 1104 31 0.0800913497967457 1104 32 -0.06056445708137659 1104 33 0.03786903685523413 1104 34 -0.018250326934129646 1104 35 -0.040545222398129965 1104 36 -0.0673086169561472 1104 37 0.07533105803454987 1104 38 0.04507495282088314 1104 39 0.038603634016688204 1104 40 0.0331430064704179 1104 41 0.0791058606313797 1104 42 0.041138340674841345 1104 43 -0.010447419449334916 1104 44 0.09844181895347504 1104 45 0.09986350987437476 1104 46 -0.16869403481200607 1104 47 0.0743073528612328 1104 48 -0.3329133695562092 1104 49 0.07193269970348126 1104 50 -0.028465279173424562 1104 51 -0.15581634550805745 1104 52 0.030187840956585887 1104 53 -0.20887557438894788 1104 54 0.02675197286822595 1104 55 0.07577964930786862 1104 56 0.06594908362036733 1104 57 0.3343300533939839 1104 58 -0.12154348744214616 1104 59 -0.16231221202568946 1104 60 -0.22232724507310309 1104 61 -0.2262836461491773 1201 1 0.07956198883288579 1201 2 0.17510747521578196 1201 3 0.1842543058058439 1201 4 0.12705622285035645 1201 5 0.08387627888757265 1201 6 0.15585618655796196 1201 7 -0.008501039376660913 1201 8 0.042078178451980364 1201 9 0.029388898920728934 1201 10 0.11720354936225164 1201 11 0.040913534753777014 1201 12 0.05475122106518171 1201 13 0.0597878728196406 1201 14 -0.03207629815924662 1201 15 0.13488661727964768 1201 16 0.13396405884756035 1201 17 0.07760108163060409 1201 18 0.07670916385372806 1201 19 0.09919947112229721 1201 20 0.20153875010177558 1201 21 0.04680640470611763 1201 22 0.08408214682450676 1201 23 -0.0644950890183793 1201 24 0.04565265350284875 1201 25 -0.026687341897726924 1201 26 0.04358179964373221 1201 27 0.17764258462517546 1201 28 0.17083447631829785 1201 29 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4 -0.09460196843073732 1202 5 -0.0427591124126252 1202 6 -0.10937936350649835 1202 7 0.2020981166659297 1202 8 0.011889839866292107 1202 9 0.02522506733022567 1202 10 0.034345464321901886 1202 11 0.04414029906644146 1202 12 -0.009867625327409257 1202 13 0.09834043214734578 1202 14 0.011993468588592293 1202 15 -0.0089927517219337 1202 16 -0.07392834553576932 1202 17 -0.019437606713950117 1202 18 -0.14244073109924216 1202 19 -0.14884656454594847 1202 20 -0.0578990692173349 1202 21 -0.010247264777717646 1202 22 -0.047538502079660816 1202 23 -0.005332272086704393 1202 24 0.04697170998949929 1202 25 -0.1035044765011719 1202 26 -0.0441041216664928 1202 27 0.11386456213556073 1202 28 0.08280834586898322 1202 29 -0.02105240967158295 1202 30 -0.09733404327092643 1202 31 0.050739010615458824 1202 32 0.20757589379581276 1202 33 0.04058541112424052 1202 34 0.01761443914774219 1202 35 0.10157539163695617 1202 36 0.02205907310233357 1202 37 -0.053607538456681425 1202 38 0.02524161948754511 1202 39 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28 -0.03218185238519311 2304 29 0.06527230552297425 2304 30 0.01622056909776859 2304 31 0.0130346534620287 2304 32 0.05023013258853837 2304 33 -0.14961469244138215 2304 34 -0.06660428773493265 2304 35 0.11789391138839989 2304 36 0.005509380100406247 2304 37 0.04723023340306298 2304 38 0.06643669272323649 2304 39 0.12244487493523337 2304 40 0.09918229910947218 2304 41 0.10592764211571648 2304 42 0.0282268075143719 2304 43 -0.04651254898422508 2304 44 0.10622746148857232 2304 45 -0.10305358312443502 2304 46 -0.02206082061806569 2304 47 -0.35336176667529173 2304 48 0.36128262642576203 2304 49 0.035395124073325235 2304 50 -0.02857666282562832 2304 51 0.11783908467860016 2304 52 0.11825792295251603 2304 53 0.09620350146313683 2304 54 0.07175246344220199 2304 55 -0.11863864676520564 2304 56 -0.07759603750467647 2304 57 0.022959670045466396 2304 58 0.13799686810718015 2304 59 0.09586089050426949 2304 60 0.004813923427377631 2304 61 0.2468128100378948 2401 1 -0.01164337368847157 2401 2 -0.11252584550052092 2401 3 0.01398021273771377 2401 4 -0.002926103824407278 2401 5 0.11332992364329897 2401 6 0.054879742453763924 2401 7 -0.020255176196471325 2401 8 -0.01943144416608078 2401 9 -0.04099779874777454 2401 10 -0.025921735825264136 2401 11 0.09183851466614357 2401 12 -0.07306666874168144 2401 13 0.02283914443579249 2401 14 0.036016187499555485 2401 15 0.005183572781376711 2401 16 0.019000738349440383 2401 17 -0.23586052797958246 2401 18 -0.012303681798279342 2401 19 0.15492689020018702 2401 20 -0.01330285876846915 2401 21 -0.006365734240560528 2401 22 -0.0712829833158608 2401 23 -0.06850142219708033 2401 24 0.07491505068118443 2401 25 0.00538533218168169 2401 26 0.09276581946669182 2401 27 -0.08915006258850156 2401 28 -0.03291553198237551 2401 29 -0.024055536971061997 2401 30 1.5721868639673175E-4 2401 31 0.07701292525857895 2401 32 -0.112291655873058 2401 33 -0.03230826069327719 2401 34 -0.02348885371466025 2401 35 -0.04027753330785089 2401 36 0.023910158459209185 2401 37 -0.004849260310983539 2401 38 -0.088858822013556 2401 39 -0.03619687719511688 2401 40 0.10765723907179137 2401 41 0.12913025937658773 2401 42 -0.011551795261027564 2401 43 0.09646091097938836 2401 44 0.1661634408891075 2401 45 0.15081366672345098 2401 46 0.06266741517056573 2401 47 0.11733837412299408 2401 48 0.2439714916033182 2401 49 0.1687766639186563 2401 50 -0.06006374553800953 2401 51 0.06520933126666506 2401 52 0.06053709156655174 2401 53 0.2934504393208286 2401 54 0.2820715964304483 2401 55 0.31436870472787604 2401 56 0.4482500014779305 2401 57 0.20066768555385295 2401 58 0.5588961547095863 2401 59 0.21045599989484032 2401 60 0.2299594982753348 2401 61 0.3174412289524895 2402 1 0.004558508828594654 2402 2 -0.11391152769376589 2402 3 2.301615929454215E-4 2402 4 -0.025457857417691798 2402 5 0.013078388813227093 2402 6 -0.13719544911991477 2402 7 -0.13109884077253042 2402 8 -0.02789361360041198 2402 9 0.04586686148536501 2402 10 0.06268866575369714 2402 11 -0.015544841170886059 2402 12 0.040288057716622916 2402 13 0.039110388997393386 2402 14 -0.03426860604120208 2402 15 -0.020940199500692813 2402 16 -0.02126120078731475 2402 17 0.00288207064213708 2402 18 0.009242974892721634 2402 19 0.03339913271795325 2402 20 0.003096198998811417 2402 21 -0.030682196620418267 2402 22 0.024477164292804332 2402 23 -0.00986945213966095 2402 24 -0.17543438984377957 2402 25 -0.011801647237668078 2402 26 -0.0781368028472329 2402 27 -0.06579765469292198 2402 28 0.1282475681892734 2402 29 0.11049669790908467 2402 30 0.03251266409398568 2402 31 0.07569426706343307 2402 32 0.02237436393409098 2402 33 -0.10952614129313787 2402 34 0.07584694625767696 2402 35 0.07150680528803122 2402 36 -0.06385620835300188 2402 37 0.06276860158450859 2402 38 0.03890372598945646 2402 39 0.01906129184174432 2402 40 0.09600285689082 2402 41 -0.013254431095283792 2402 42 0.051054074404575545 2402 43 -0.02643850041742013 2402 44 0.0487405136159893 2402 45 0.07564313546084378 2402 46 0.04600415815497172 2402 47 -0.10388524248414654 2402 48 -0.30222967001609646 2402 49 -0.11246382559033208 2402 50 0.10531796976976437 2402 51 0.029515934884010164 2402 52 0.02486317890427614 2402 53 -0.3033281223519708 2402 54 0.01750293677402226 2402 55 -0.32083409157567644 2402 56 0.1749612079293783 2402 57 -0.019530308012619457 2402 58 0.23439946791624067 2402 59 -0.10485060567088311 2402 60 0.28568371790362335 2402 61 0.028941884579680277 2403 1 -0.06881979185887133 2403 2 0.14638243741894286 2403 3 0.1276618285276001 2403 4 0.05239635689327571 2403 5 -0.012523929654506105 2403 6 0.1851474495122858 2403 7 0.1561878756582118 2403 8 0.10979239920807515 2403 9 0.002710231885345138 2403 10 -0.04558618341364706 2403 11 0.08487633736555011 2403 12 -0.03607084361777879 2403 13 0.10011977538310646 2403 14 0.03498158202968682 2403 15 0.12544777907754678 2403 16 0.12489619574863298 2403 17 0.001700626866949051 2403 18 0.04177817817701168 2403 19 0.051315897227752316 2403 20 0.17361123368058884 2403 21 -0.01791072771641211 2403 22 0.019507697133967383 2403 23 -0.01404923364196975 2403 24 0.04389540301411188 2403 25 0.004207224387130282 2403 26 0.1616307334376259 2403 27 0.11268364252000396 2403 28 0.08971183356376145 2403 29 0.15309113918527117 2403 30 0.10309236200066607 2403 31 0.10335023094457085 2403 32 0.013386291349334556 2403 33 -0.03554863576668765 2403 34 0.057890851700527435 2403 35 0.09046928635081031 2403 36 0.08809273044906263 2403 37 0.09009604389369485 2403 38 0.12443886719396602 2403 39 -0.03918496592352483 2403 40 0.08240082992814735 2403 41 0.1905330524641755 2403 42 0.02372894562758887 2403 43 0.04064545033981659 2403 44 -0.15971182202972045 2403 45 -0.004745167783077875 2403 46 0.23819621834382332 2403 47 0.08980444421346334 2403 48 -0.43082273011356165 2403 49 -0.18202402697560416 2403 50 0.07716613882110579 2403 51 0.008787457577639657 2403 52 -0.13696839826532425 2403 53 0.20467573507738024 2403 54 0.08622101243963698 2403 55 -0.1543376623198256 2403 56 -0.18195434467514104 2403 57 -0.5312011139739781 2403 58 -0.33207274636928474 2403 59 -0.38353021115194963 2403 60 -0.3718290730375102 2403 61 -0.10271969038821283 2404 1 -0.12084826413307326 2404 2 -0.08889217198954213 2404 3 -0.10542185562576378 2404 4 -0.14360666763401753 2404 5 -0.11461279305763128 2404 6 -0.2008147971690164 2404 7 -0.1555027551913284 2404 8 -0.13760132825545668 2404 9 -0.10563477356477335 2404 10 -0.0711622344997167 2404 11 -0.10445336893604207 2404 12 -0.18506501571908274 2404 13 -0.14559324229566784 2404 14 -0.12241313772789653 2404 15 -0.03456479623316966 2404 16 -0.15916476674659208 2404 17 -0.15711144145465994 2404 18 -0.032577473143987845 2404 19 -0.19734511542615252 2404 20 -0.06557552787865717 2404 21 -0.23069834058517102 2404 22 -0.05087952827116027 2404 23 -0.04315027337481325 2404 24 -0.0516101801874953 2404 25 -0.22326408959214916 2404 26 -0.1360799935237509 2404 27 -0.07426830962628372 2404 28 -0.4689320887476853 2404 29 -0.10593475068584723 2404 30 -0.16630215667200332 2404 31 -0.16138857156096265 2404 32 -0.32276617003702057 2404 33 -0.0986225226575499 2404 34 -0.15999591460446538 2404 35 -0.27754162925899095 2404 36 -0.15137089803454273 2404 37 -0.32850198528650765 2404 38 -0.04736643833412302 2404 39 -0.20890589119178316 2404 40 -0.193830576047489 2404 41 -0.12213524126462261 2404 42 -0.22136130905059304 2404 43 -0.27252330474006214 2404 44 -0.22419405368217582 2404 45 -0.1658592082562933 2404 46 -0.2862257516093649 2404 47 -0.5261711899965604 2404 48 -0.3297843103259055 2404 49 -0.3939630314664342 2404 50 -0.353615535664801 2404 51 -0.15651635228756078 2404 52 -0.5034704266531875 2404 53 -0.3021760647733416 2404 54 -0.46365845853445364 2404 55 -0.3501441038276525 2404 56 -0.49525965742503075 2404 57 -0.7224726403304516 2404 58 -0.5608150971232888 2404 59 -0.37650199304759013 2404 60 -0.2881387295593285 2404 61 -0.2404501056642161 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 6.0 14 19.0 15 76.0 16 259.0 17 557.0 18 787.0 19 804.0 20 991.0 21 997.0 22 1158.0 23 1389.0 24 1704.0 25 1888.0 26 2234.0 27 2771.0 28 3974.0 29 4853.0 30 6375.0 31 8411.0 32 11285.0 33 12714.0 34 17037.0 35 25355.0 36 37232.0 37 52421.0 38 97799.0 39 249740.0 40 191629.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.909360622725334 29.783007397382256 18.435180659242505 21.8724513206499 2 30.64883963156856 17.02940235409448 20.21702872158646 32.104729292750505 3 31.69063656031686 17.892392128670878 18.12575990894027 32.29121140207199 4 32.116846956628294 18.86352651249549 19.081644462295685 29.93798206858053 5 29.375259542660302 19.51924189716324 19.450484366171295 31.65501419400516 6 28.149877802209772 18.984022383639793 21.951352344904112 30.914747469246322 7 30.95300661025372 21.38849366545717 20.852321077246703 26.806178647042405 8 28.454180934421654 22.905380106608213 22.200921759375873 26.439517199594263 9 28.250359104926716 22.463561912412437 22.312295344230154 26.973783638430692 10 31.259079738312924 22.14959187980367 20.81746577440722 25.77386260747619 11 29.5592029572546 22.837984110883433 20.928022438101205 26.674790493760764 12 29.658322724704377 21.06009135901643 21.384681366709103 27.896904549570095 13 30.801195427964572 21.847467204019253 20.068757530991945 27.28257983702423 14 31.204890634679668 21.614100059226786 20.2678139870518 26.913195319041748 15 29.738108691360377 21.31320076518282 21.55187789751724 27.396812645939562 16 30.79057545288067 21.99560224108705 19.59793863560551 27.615883670426772 17 31.696813326707197 21.865031008965712 19.579285602445317 26.858870061881778 18 29.61625128494891 21.28379160341201 21.388629818983887 27.7113272926552 19 32.07096321812476 22.216987875528446 19.39874602601894 26.31330288032786 20 30.497709216912995 21.987977643590913 20.826315753643808 26.687997385852285 21 30.20348144567815 22.693933679617135 19.95724779261095 27.145337082093768 22 32.203168292566694 22.495557991190868 19.18702729197443 26.114246424268007 23 31.787083114920385 22.30671304963477 19.598483249712377 26.30772058573247 24 30.79316236988829 22.01057912902589 19.970726991755903 27.22553150932992 25 31.133137726099953 23.97173452785361 19.196285731791168 25.698842014255273 26 30.50179382271449 24.060778934326347 19.469545859911637 25.967881383047526 27 30.196129155235447 23.96778607557882 19.669963851238656 26.166120917947076 28 30.999843423444272 24.89444697841286 18.873874180525963 25.231835417616903 29 30.977786552116164 24.787430306413512 19.034535342051697 25.200247799418623 30 30.24936518418168 24.495380991606137 19.500044249896185 25.755209574316 31 31.629417330982417 24.35500670556119 19.674048457040158 24.341527506416234 32 32.213243653543735 24.5650915972851 18.835478885991844 24.38618586317932 33 30.926154868365156 24.226774646813425 19.888733974533533 24.958336510287886 34 31.643985758341103 25.01181131844268 18.37909226443738 24.965110658778837 35 31.501705322922124 25.263286882288465 18.59326176196279 24.641746032826614 36 31.247370535015285 25.53967854152342 18.82077430510644 24.392176618354856 37 32.66513720871655 26.179055502985165 17.99813469668398 23.157672591614304 38 32.22495285684138 25.330274417433095 18.764270591518997 23.68050213420653 39 32.38207402667248 25.033323575663918 18.182758878911862 24.401843518751747 40 33.2109766973239 25.349199757646723 17.721062269815445 23.718761275213932 41 33.23208049396499 25.51108630091291 18.9271102094722 22.329722995649895 42 32.98727645292832 25.132034882533542 19.161838889531836 22.718849775006298 43 33.81785901010805 25.795273832345767 18.49729871035204 21.889568447194144 44 33.53788131497076 25.79292410121653 19.102067491303192 21.567127092509512 45 34.028578625257836 25.464113334195638 18.803074346633263 21.70423369391326 46 34.72641273091669 25.458701856047405 17.108939308121297 22.705946104914602 47 34.812414478566026 25.471874085218495 17.18856582682633 22.527145609389144 48 34.417977711667675 26.81475631922556 17.536438087587563 21.2308278815192 49 35.76657839379684 25.91301151178068 16.613317176448163 21.707092917974308 50 34.688650922780525 27.115927920322957 17.68743234871641 20.5079888081801 51 34.57196735038429 27.214911534246017 17.415942216443263 20.79717889892643 52 34.866195121619135 27.805137072563024 17.361753112810003 19.966914693007837 53 35.371333030163576 25.928911230261058 16.696847488365634 22.00290825120973 54 34.31572641310341 25.584473051813223 16.55395423879967 23.545846296283692 55 35.469082937920795 26.26455991776327 17.55618034896149 20.710176795354442 56 33.77437999087771 27.4418794632828 19.45198205496518 19.33175849087431 57 34.28513855001743 28.588194928546535 18.37435190833043 18.75231461310561 58 33.16577372645395 29.764930936123573 18.847868856923068 18.22142648049941 59 32.83532911711246 30.202256063937693 17.915625659493646 19.046789159456203 60 31.874029899518014 27.640034855540126 21.52629142499251 18.959643819949353 61 32.46676152029028 29.83151001068805 18.519466550482324 19.182261918539346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.5 7 1.5 8 0.0 9 0.0 10 0.0 11 0.0 12 1.5 13 3.0 14 2.0 15 1.0 16 5.0 17 7.0 18 5.0 19 9.0 20 11.0 21 9.0 22 19.0 23 29.0 24 50.5 25 92.5 26 113.0 27 175.5 28 238.0 29 311.0 30 505.5 31 627.0 32 835.0 33 1043.0 34 1391.0 35 2195.0 36 2651.0 37 3297.0 38 3943.0 39 4822.5 40 6958.5 41 8215.0 42 9841.5 43 13459.5 44 15451.0 45 18565.5 46 21680.0 47 25248.0 48 33248.0 49 37680.0 50 41747.5 51 45815.0 52 49511.0 53 55886.5 54 58566.0 55 60783.0 56 63000.0 57 63534.5 58 63337.0 59 62605.0 60 59472.5 61 53031.0 62 49722.0 63 45277.5 64 40833.0 65 36222.0 66 27589.5 67 23568.0 68 20070.5 69 16573.0 70 13811.0 71 9036.0 72 7023.0 73 5572.5 74 4122.0 75 3347.5 76 2042.5 77 1512.0 78 1156.5 79 801.0 80 636.0 81 368.0 82 265.0 83 207.0 84 108.0 85 67.0 86 61.5 87 56.0 88 47.0 89 35.0 90 32.0 91 32.0 92 32.0 93 37.5 94 39.5 95 36.0 96 34.5 97 33.0 98 42.0 99 180.0 100 309.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021784564274676126 2 0.0 3 2.7230705343345157E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.001225381740450532 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 1.3615352671672579E-4 44 0.0 45 0.0 46 1.3615352671672579E-4 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 4.084605801501773E-4 54 0.0 55 0.0 56 0.0 57 1.3615352671672579E-4 58 0.0 59 0.0 60 6.807676335836289E-4 61 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 734465.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.65454838112659 #Duplication Level Percentage of deduplicated Percentage of total 1 78.37521506970921 58.51066285302807 2 14.996399788326405 22.3909890708066 3 4.423916090431561 9.907963734215022 4 1.3131070993003309 3.9211766989726935 5 0.4328519142415337 1.6157182086803914 6 0.1688120872219565 0.7561554079698308 7 0.08943961990413846 0.4673952101926154 8 0.04800084713370097 0.28667852517423525 9 0.03134212073415106 0.2105848681843312 >10 0.11625846414372337 1.2900862945232758 >50 0.0023284494266520824 0.12110110712791969 >100 0.0017463370699890616 0.29174357626271563 >500 5.821123566630206E-4 0.2297444448622974 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.0517859938867067 0.0 0.0 0.0 0.0 2 1.0532836826805907 0.0 0.0 0.0 0.0 3 1.054645217947758 0.0 0.0 0.0 0.0 4 1.0558705996882085 0.0 0.0 0.0 0.0 5 1.0569598279019423 0.0 0.0 0.0 0.0 6 1.0594105913828433 0.0 0.0 0.0 0.0 7 1.0628144295507613 0.0 0.0 0.0 0.0 8 1.0708474876270482 0.0 0.0 0.0 0.0 9 1.0745236328483998 0.0 0.0 0.0 0.0 10 1.0806505415506524 0.0 0.0 0.0 0.0 11 1.093857433642175 0.0 0.0 0.0 0.0 12 1.1093789356878816 0.0 0.0 0.0 0.0 13 1.1349757987106261 0.0 0.0 0.0 0.0 14 1.1765026243592274 0.0 0.0 0.0 0.0 15 1.2364101761145867 0.0 0.0 0.0 0.0 16 1.3178299850911888 0.0 4.0846058015017733E-4 0.0 0.0 17 1.4341050969072726 0.0 4.0846058015017733E-4 0.0 0.0 18 1.6074285364176646 0.0 4.0846058015017733E-4 0.0 0.0 19 1.8552279550421056 0.0 4.0846058015017733E-4 0.0 0.0 20 2.177639506307312 0.0 4.0846058015017733E-4 0.0 0.0 21 2.5826962482895715 0.0 4.0846058015017733E-4 0.0 1.3615352671672579E-4 22 3.0574635959507943 0.0 4.0846058015017733E-4 0.0 1.3615352671672579E-4 23 3.604256159245165 0.0 4.0846058015017733E-4 0.0 1.3615352671672579E-4 24 4.282164568767742 0.0 4.0846058015017733E-4 0.0 2.7230705343345157E-4 25 5.025835131694499 0.0 4.0846058015017733E-4 0.0 9.530746870170804E-4 26 5.894630785673926 0.0 4.0846058015017733E-4 0.0 9.530746870170804E-4 27 6.865541584690898 0.0 5.446141068669031E-4 0.0 9.530746870170804E-4 28 7.956267487218588 0.0 5.446141068669031E-4 0.0 9.530746870170804E-4 29 9.135084721532 0.0 5.446141068669031E-4 0.0 9.530746870170804E-4 30 10.454003934836923 0.0 5.446141068669031E-4 0.0 9.530746870170804E-4 31 11.82343610655375 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 32 13.344270999979576 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 33 14.954967221038443 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 34 16.617401782249665 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 35 18.35199771262075 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 36 20.24616557630384 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 37 22.03141061861355 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 38 23.931024623365307 4.0846058015017733E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 39 25.930847623780576 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 40 27.919369881478357 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 41 29.944108977282784 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 42 32.028755624842574 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 43 34.09352385750172 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 44 36.123845247901535 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 45 38.198824995064435 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 46 40.23159714894515 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 47 42.24639703729926 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 48 44.33921289646205 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 49 46.32896053590028 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 50 48.35329117112456 5.446141068669031E-4 5.446141068669031E-4 0.0 9.530746870170804E-4 >>END_MODULE