##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-22C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 372921 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 57 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.54939518021243 40.0 40.0 40.0 40.0 40.0 2 39.302318721659546 40.0 40.0 40.0 40.0 40.0 3 39.280169258368396 40.0 40.0 40.0 40.0 40.0 4 39.114707940823926 40.0 40.0 40.0 40.0 40.0 5 39.198307416316055 40.0 40.0 40.0 40.0 40.0 6 39.34210194652487 40.0 40.0 40.0 40.0 40.0 7 39.365431284373905 40.0 40.0 40.0 40.0 40.0 8 39.39818889255365 40.0 40.0 40.0 40.0 40.0 9 39.38030842993556 40.0 40.0 40.0 40.0 40.0 10-11 39.38914139992116 40.0 40.0 40.0 40.0 40.0 12-13 39.30347178088657 40.0 40.0 40.0 40.0 40.0 14-15 39.34903639108551 40.0 40.0 40.0 40.0 40.0 16-17 39.44816730621231 40.0 40.0 40.0 40.0 40.0 18-19 39.48270545236122 40.0 40.0 40.0 40.0 40.0 20-21 39.465195041309016 40.0 40.0 40.0 40.0 40.0 22-23 39.507568627135505 40.0 40.0 40.0 40.0 40.0 24-25 39.481541667001856 40.0 40.0 40.0 40.0 40.0 26-27 39.453439200259574 40.0 40.0 40.0 40.0 40.0 28-29 39.47225283639162 40.0 40.0 40.0 40.0 40.0 30-31 39.44606853433339 40.0 40.0 40.0 40.0 40.0 32-33 39.44321782760325 40.0 40.0 40.0 40.0 40.0 34-35 39.44521709898788 40.0 40.0 40.0 40.0 40.0 36-37 39.4285055433012 40.0 40.0 40.0 40.0 40.0 38-39 39.35169362252033 40.0 40.0 40.0 40.0 40.0 40-41 39.405978727679106 40.0 40.0 40.0 40.0 40.0 42-43 39.38608670131413 40.0 40.0 40.0 40.0 40.0 44-45 39.274370930793765 40.0 40.0 40.0 40.0 40.0 46-47 39.26958160232595 40.0 40.0 40.0 40.0 40.0 48-49 39.35769218226511 40.0 40.0 40.0 40.0 40.0 50-51 39.32083365020617 40.0 40.0 40.0 40.0 40.0 52-53 39.272100808499566 40.0 40.0 40.0 40.0 40.0 54-55 39.18191224461447 40.0 40.0 40.0 40.0 40.0 56-57 39.208836435917036 40.0 40.0 40.0 40.0 40.0 58-59 39.154331181884764 40.0 40.0 40.0 40.0 40.0 60-61 39.06690066403273 40.0 40.0 40.0 40.0 40.0 62-63 39.3291219368685 40.0 40.0 40.0 40.0 40.0 64-65 39.33737952105149 40.0 40.0 40.0 40.0 40.0 66-67 39.34327444077444 40.0 40.0 40.0 40.0 40.0 68-69 39.341616881855664 40.0 40.0 40.0 40.0 40.0 70-71 39.34235712172806 40.0 40.0 40.0 40.0 40.0 72-73 39.335579381560166 40.0 40.0 40.0 40.0 40.0 74-75 39.34374585572455 40.0 40.0 40.0 40.0 40.0 76-77 39.36061752627828 40.0 40.0 40.0 40.0 40.0 78-79 39.345711266564976 40.0 40.0 40.0 40.0 40.0 80-81 39.312959158741776 40.0 40.0 40.0 40.0 40.0 82-83 39.35692896228038 40.0 40.0 40.0 40.0 40.0 84-85 39.32967207861353 40.0 40.0 40.0 40.0 40.0 86-87 39.287377652277755 40.0 40.0 40.0 40.0 40.0 88-89 39.35859375294502 40.0 40.0 40.0 40.0 40.0 90-91 39.49468510874894 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.04582354580477954 1101 2 -0.2412333533055815 1101 3 0.10143087761809255 1101 4 -0.14842848750951276 1101 5 0.2242993153123365 1101 6 0.09548944323689312 1101 7 0.2636873542348397 1101 8 0.24916558080664686 1101 9 0.17068356660082173 1101 10-11 0.07736932218254822 1101 12-13 0.11084774554852572 1101 14-15 -0.009139010993770569 1101 16-17 -0.08352613524840535 1101 18-19 -0.008166427679100252 1101 20-21 -0.051673051142088866 1101 22-23 0.04173138872844362 1101 24-25 0.03963085205126049 1101 26-27 -0.13619736378451108 1101 28-29 -0.0655446418785246 1101 30-31 -0.06965872459977618 1101 32-33 -0.03499979531402175 1101 34-35 -0.030670779682303362 1101 36-37 0.035517715983750975 1101 38-39 0.010690645421128409 1101 40-41 -0.1717427357828285 1101 42-43 -0.03705449073933664 1101 44-45 -0.07597930237857042 1101 46-47 1.0280993043920716E-4 1101 48-49 0.0662665027958198 1101 50-51 -0.1494574200489751 1101 52-53 -0.21477668305406894 1101 54-55 -0.028155426941182782 1101 56-57 -0.10092865934952044 1101 58-59 -0.06959565148031288 1101 60-61 0.11693868094320692 1101 62-63 -0.10417624718616025 1101 64-65 -0.009724102188314987 1101 66-67 -0.026112599097139366 1101 68-69 -0.26054455321934 1101 70-71 -0.3123322513258273 1101 72-73 0.08070528871350291 1101 74-75 0.09399776338011634 1101 76-77 -0.21730961867693566 1101 78-79 -0.29532742563825565 1101 80-81 -0.18814221290305966 1101 82-83 0.26116530711952635 1101 84-85 -0.970015709801423 1101 86-87 0.6756184173500941 1101 88-89 -0.7710580979823547 1101 90-91 -1.8393386994949523 1102 1 0.08000748661467583 1102 2 -0.20309705750057105 1102 3 0.029506833007999944 1102 4 0.11629652024030435 1102 5 -0.07981060949644814 1102 6 0.021281546356284764 1102 7 -0.0015163397231887643 1102 8 -0.12089922926882934 1102 9 -0.007629929610786235 1102 10-11 -0.02905184958937923 1102 12-13 0.008956790843484441 1102 14-15 -0.0330214156710511 1102 16-17 0.11654739787394419 1102 18-19 -0.002505819084376526 1102 20-21 -0.11533831683455986 1102 22-23 -0.0025182490127946267 1102 24-25 -0.003165425154193713 1102 26-27 0.008028483828908861 1102 28-29 -0.1439338339513654 1102 30-31 -0.2028949749872737 1102 32-33 -0.054660839461583066 1102 34-35 0.05295039950630809 1102 36-37 -0.18834397257113977 1102 38-39 0.08859827224551253 1102 40-41 -0.08586648132450847 1102 42-43 -0.0625590291089253 1102 44-45 -0.13729196517624587 1102 46-47 -0.09684554205986018 1102 48-49 -0.022609831013653547 1102 50-51 -0.1804290395351984 1102 52-53 -0.16353861554308935 1102 54-55 -0.05986592078704689 1102 56-57 0.1753995781022013 1102 58-59 -0.18372287508194773 1102 60-61 -0.2726942233980836 1102 62-63 -0.2989217722851123 1102 64-65 -0.13906942505099806 1102 66-67 -0.06058433221267734 1102 68-69 0.003817762383398815 1102 70-71 -0.4635210723702272 1102 72-73 -0.6274635276416802 1102 74-75 -0.5779210041268854 1102 76-77 -0.705523628557998 1102 78-79 -0.559497802641701 1102 80-81 0.12150411561119512 1102 82-83 -0.07488310760281536 1102 84-85 -0.034906942497535454 1102 86-87 0.1756184173500941 1102 88-89 -1.3742327011569628 1102 90-91 0.8273279671717191 1103 1 0.16276822868754692 1103 2 -0.02126195029232747 1103 3 -0.1380005635714312 1103 4 -0.11858619355221123 1103 5 0.03254454503910864 1103 6 -0.15659792548814266 1103 7 -0.11029361397742576 1103 8 0.11637413454573675 1103 9 -0.13553240404148426 1103 10-11 -0.08309780889166518 1103 12-13 -0.11978483952046304 1103 14-15 -0.4008388412431856 1103 16-17 -0.05983994139464244 1103 18-19 -0.006706995251811065 1103 20-21 -0.05173785219580651 1103 22-23 -0.07144506031468723 1103 24-25 -0.0874102050511425 1103 26-27 -0.039207090986160154 1103 28-29 0.009218869493359705 1103 30-31 -0.06371111484299519 1103 32-33 -0.09673088982299305 1103 34-35 -0.09005595936277189 1103 36-37 -0.013504229962606473 1103 38-39 -0.19318007606497645 1103 40-41 -0.2576127041309988 1103 42-43 -0.011894295563990909 1103 44-45 -0.11914657496467385 1103 46-47 -0.013197623934011915 1103 48-49 0.0038125560043624773 1103 50-51 -0.13278637429795737 1103 52-53 -0.025812676230344778 1103 54-55 0.013357255901951248 1103 56-57 -0.16260739359462661 1103 58-59 -0.2768894021479156 1103 60-61 -0.1030539361207019 1103 62-63 0.19487371173097756 1103 64-65 -0.14549392769503555 1103 66-67 -0.0307787871804166 1103 68-69 -0.05099080718058957 1103 70-71 0.03862924099160381 1103 72-73 -0.2453739549509777 1103 74-75 -0.02667323229087515 1103 76-77 0.08293132614275578 1103 78-79 0.3641211735482415 1103 80-81 0.3987470882719606 1103 82-83 0.04866001594031388 1103 84-85 0.6634714358808438 1103 86-87 0.6756184173500941 1103 88-89 0.7527514258271637 1103 90-91 0.8273279671717191 1104 1 0.0908676381426119 1104 2 0.14344617081061273 1104 3 0.08483595506784525 1104 4 -0.22223793061699126 1104 5 0.004416591521533064 1104 6 -0.001612546391278613 1104 7 -0.15202630200350598 1104 8 -0.3069812517188595 1104 9 0.08299078959509387 1104 10-11 -0.10982520040837329 1104 12-13 0.09673392661485991 1104 14-15 -0.06528245403345778 1104 16-17 0.019961645655229177 1104 18-19 -0.10646151631415535 1104 20-21 -0.07537233596058002 1104 22-23 0.029420776248358038 1104 24-25 -0.039981390771501424 1104 26-27 0.06915124483156632 1104 28-29 0.01094927399443435 1104 30-31 0.0370151729699586 1104 32-33 -0.06840476846871013 1104 34-35 0.05275029074710602 1104 36-37 -0.04442024382097287 1104 38-39 0.08701361300603594 1104 40-41 -0.04856235045580348 1104 42-43 0.028738618902949042 1104 44-45 -0.08139617089648254 1104 46-47 -0.06155945128868012 1104 48-49 0.08654289454315034 1104 50-51 0.10266030171302276 1104 52-53 -0.020342482432340603 1104 54-55 0.23386502014268729 1104 56-57 0.0951616294293487 1104 58-59 0.3024489527207592 1104 60-61 0.19433399489854963 1104 62-63 -0.10850045898312999 1104 64-65 -0.23481752494792119 1104 66-67 -0.2908532348055175 1104 68-69 -0.39582293511147526 1104 70-71 -0.12317435078655592 1104 72-73 0.2259285272039051 1104 74-75 0.039016560372601816 1104 76-77 0.02709596251638402 1104 78-79 0.20796705901542367 1104 80-81 0.4713094465486023 1104 82-83 0.023109339226841996 1104 84-85 0.023471435880843217 1104 86-87 -0.9664868458078004 1104 88-89 -0.3139152408394992 1104 90-91 -7.172672032828281 1201 1 0.09312669277736063 1201 2 0.24471075030723455 1201 3 0.24122637864059016 1201 4 0.2515987790208243 1201 5 0.1688119260826113 1201 6 0.09685276975359614 1201 7 0.0034555887270002472 1201 8 0.09235576485572494 1201 9 0.03555064226544147 1201 10-11 0.043913289462707894 1201 12-13 0.035592121826638845 1201 14-15 0.019640811773783184 1201 16-17 0.012656550504829056 1201 18-19 -0.16768926339825896 1201 20-21 0.16112858484973458 1201 22-23 -0.13539199232131693 1201 24-25 -0.04535892299986699 1201 26-27 0.11758586729933285 1201 28-29 -0.003022487822626374 1201 30-31 -0.05699342125963369 1201 32-33 -0.07927332495789585 1201 34-35 0.09030007989586863 1201 36-37 -0.06221664900260038 1201 38-39 0.006320631216709671 1201 40-41 0.068481856570763 1201 42-43 0.03394218685420469 1201 44-45 -0.16755681224564967 1201 46-47 -0.10447969809957414 1201 48-49 -0.10851522165341976 1201 50-51 0.010881185186640607 1201 52-53 0.11346055777659814 1201 54-55 -0.045103754697159104 1201 56-57 -0.13656028435767098 1201 58-59 -0.15625080562583804 1201 60-61 -0.07255853286353897 1201 62-63 -0.14049419789409967 1201 64-65 -0.142191803462687 1201 66-67 0.08621579722441908 1201 68-69 -0.0816113046162883 1201 70-71 0.10332557835535994 1201 72-73 0.354652136324475 1201 74-75 0.15443732381967834 1201 76-77 0.21205857687316154 1201 78-79 0.33331154373364313 1201 80-81 0.4274053600913277 1201 82-83 0.2271806822658533 1201 84-85 0.12500989741930368 1201 86-87 0.20503018205597812 1201 88-89 0.7527514258271637 1201 90-91 0.8273279671717191 1202 1 -0.15893400227893295 1202 2 0.03740776450204919 1202 3 0.07376676043207198 1202 4 -0.05557694295666238 1202 5 -0.028153321102898587 1202 6 -0.021404694759375786 1202 7 -0.1405287480326649 1202 8 -0.2246486404306154 1202 9 0.01869307985721491 1202 10-11 -0.01931725385593097 1202 12-13 -0.04663988992078316 1202 14-15 -0.04089761211265852 1202 16-17 -0.07305074269144995 1202 18-19 -0.12423356099982641 1202 20-21 -0.17681561485657937 1202 22-23 -0.021902026584655232 1202 24-25 -0.08436039142864615 1202 26-27 0.048716557460863896 1202 28-29 -0.007173246510831177 1202 30-31 0.08939913870121075 1202 32-33 -0.1651802504906641 1202 34-35 -0.05777690631822452 1202 36-37 -0.14266004329718385 1202 38-39 -0.3085118501126729 1202 40-41 -0.13145789703472843 1202 42-43 -0.2306035775683597 1202 44-45 -0.2216850281195306 1202 46-47 -0.14396995543831537 1202 48-49 -0.13341208401524796 1202 50-51 -0.20877118783506177 1202 52-53 0.016144314292333206 1202 54-55 -0.17326329332748003 1202 56-57 0.25813840786227615 1202 58-59 -0.07477218256634188 1202 60-61 -0.006030295384839235 1202 62-63 0.1405349702285008 1202 64-65 0.2382551541489093 1202 66-67 0.10554867533063117 1202 68-69 0.31672104476728435 1202 70-71 0.2359815531221443 1202 72-73 0.2761122718897724 1202 74-75 0.3365509341624602 1202 76-77 -0.01598296241743924 1202 78-79 -0.1587183326245949 1202 80-81 0.5714315466707021 1202 82-83 0.4068982689154055 1202 84-85 -0.043671421262011734 1202 86-87 0.6756184173500941 1202 88-89 -0.025026351950614867 1202 90-91 0.10005523989899245 1203 1 0.0707356941838242 1203 2 0.032277557620879804 1203 3 0.051015408176454 1203 4 0.2247070771924129 1203 5 -0.14454953664593972 1203 6 -0.05954666765850192 1203 7 0.007224927686216631 1203 8 -0.0036779904185308965 1203 9 0.03993882791944969 1203 10-11 -0.057858862436020786 1203 12-13 -0.015040789565759383 1203 14-15 -0.07509899132045206 1203 16-17 0.0517564098578589 1203 18-19 -0.1542434124840497 1203 20-21 -0.07844947422199766 1203 22-23 -0.09288847614691065 1203 24-25 -0.15135048418129315 1203 26-27 -0.04612721286944321 1203 28-29 -0.1661167212966106 1203 30-31 -0.3000876547336091 1203 32-33 -0.08971406667150461 1203 34-35 -0.10859015787416837 1203 36-37 0.009036234227366435 1203 38-39 -0.03143196891369371 1203 40-41 -0.03726264863698958 1203 42-43 -0.08130574026694148 1203 44-45 -0.2557300214061442 1203 46-47 -0.03276514181963108 1203 48-49 -0.08933835662739398 1203 50-51 -0.1084875983065885 1203 52-53 -0.06572279780862544 1203 54-55 -0.1399251578670544 1203 56-57 0.07601348128120122 1203 58-59 -0.09230935178491961 1203 60-61 -0.2801827926617193 1203 62-63 -0.07897665372192364 1203 64-65 0.10926271978673441 1203 66-67 -0.05509342219560409 1203 68-69 -0.4065808726847777 1203 70-71 0.026764784850641377 1203 72-73 -0.26733207359076516 1203 74-75 0.08340454304114076 1203 76-77 0.15761632041537865 1203 78-79 0.5633343060947098 1203 80-81 -0.16361118837203037 1203 82-83 -0.4225754152951211 1203 84-85 -0.6740026842019731 1203 86-87 -1.1132704715387973 1203 88-89 0.7527514258271637 1203 90-91 0.8273279671717191 1204 1 -0.015246200239495522 1204 2 0.06773104614487124 1204 3 -0.41714307440378917 1204 4 -0.3553637252791404 1204 5 -0.05774466146468882 1204 6 -0.2376501542230116 1204 7 -0.24691936827333905 1204 8 -0.052246918322808256 1204 9 -0.11668877981918513 1204 10-11 -0.12165946595524701 1204 12-13 -0.16461427652217253 1204 14-15 0.025206687246779325 1204 16-17 -0.21021926648863598 1204 18-19 -0.18722142714094758 1204 20-21 -0.24842765870032224 1204 22-23 -0.15856364694808178 1204 24-25 0.01610650527670998 1204 26-27 -0.04969999441376416 1204 28-29 -0.08300872793271452 1204 30-31 -0.08746469748942332 1204 32-33 -0.08637713028103633 1204 34-35 -0.06105328409324784 1204 36-37 -0.03898117856159189 1204 38-39 -0.10148048264589704 1204 40-41 0.017390445586521253 1204 42-43 -0.0352828280262969 1204 44-45 -0.14370713208118957 1204 46-47 -0.07372041008547825 1204 48-49 -0.027219825539589237 1204 50-51 0.011042891688596512 1204 52-53 -0.036591785448464975 1204 54-55 0.13926040715115562 1204 56-57 0.07966962362417718 1204 58-59 -0.2676886811596475 1204 60-61 -0.15802516260599475 1204 62-63 -0.037573783776657876 1204 64-65 -0.1430928603063748 1204 66-67 -0.12666129013754102 1204 68-69 -0.07419895576888536 1204 70-71 0.1744660901678401 1204 72-73 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-0.03366743705597486 2401 50-51 0.030895334255937712 2401 52-53 0.16370451411257392 2401 54-55 0.17411474179837683 2401 56-57 0.30980901071180966 2401 58-59 0.2565815652776777 2401 60-61 0.07667324222635585 2401 62-63 0.06642509583883793 2401 64-65 0.35091510675845683 2401 66-67 0.06338969470622402 2401 68-69 0.13956107191413736 2401 70-71 0.24502886073060637 2401 72-73 0.12434123123480845 2401 74-75 -0.0742268235108412 2401 76-77 -0.3046220275594891 2401 78-79 0.2714080769135805 2401 80-81 0.29743887267802904 2401 82-83 0.4198937205073463 2401 84-85 0.5278782155418611 2401 86-87 0.4403242997030361 2401 88-89 0.7527514258271637 2401 90-91 0.16066130050504768 2402 1 -0.16259272651256396 2402 2 0.06769766995650883 2402 3 0.05668131611363947 2402 4 0.043687322933429584 2402 5 -0.053496540210332455 2402 6 0.06402252683279386 2402 7 -0.03993092844994095 2402 8 0.0767800856836729 2402 9 0.053006442179196256 2402 10-11 0.1023695205968238 2402 12-13 0.029555036876232066 2402 14-15 -0.037110661562429925 2402 16-17 0.021295331588632394 2402 18-19 0.08762480875927992 2402 20-21 0.06416437613908244 2402 22-23 0.07010462978591647 2402 24-25 0.05966910632312761 2402 26-27 -0.08038739703874143 2402 28-29 0.0873153014543604 2402 30-31 0.03204208254948071 2402 32-33 0.0852640527763242 2402 34-35 0.07874774309038912 2402 36-37 0.014907668099574778 2402 38-39 -0.38229822789634227 2402 40-41 0.04797838674520705 2402 42-43 0.11776851744713213 2402 44-45 0.17660453530450582 2402 46-47 -0.022086701301482492 2402 48-49 0.13220633540456816 2402 50-51 -0.01676854360204061 2402 52-53 -0.07877795921658048 2402 54-55 0.16028036917177246 2402 56-57 -0.2593173793211889 2402 58-59 -8.144269857766062E-4 2402 60-61 -0.054499586087786156 2402 62-63 0.016487226322865922 2402 64-65 -0.27136525089684227 2402 66-67 -0.1939133401000106 2402 68-69 0.022582748393702445 2402 70-71 -0.08485363105921095 2402 72-73 -0.09338420190054109 2402 74-75 -0.16442368356794645 2402 76-77 -0.21903995169610369 2402 78-79 -0.05649945765321718 2402 80-81 -0.16903124257257218 2402 82-83 -0.16276508863548145 2402 84-85 -0.39550292309351676 2402 86-87 -0.311516085573885 2402 88-89 0.7527514258271637 2402 90-91 -0.9453993055555543 2403 1 0.06292118946840475 2403 2 0.17412907144969836 2403 3 0.13518667122156813 2403 4 0.22257753206854147 2403 5 0.18880495986874735 2403 6 0.18058666038403715 2403 7 0.2273775671317395 2403 8 0.12081971469898889 2403 9 0.09807937789762633 2403 10-11 0.09060085477096891 2403 12-13 0.149280732066984 2403 14-15 0.05793408937740452 2403 16-17 0.06967501097157225 2403 18-19 0.03302825662960629 2403 20-21 0.08424248244638477 2403 22-23 0.1182896517051546 2403 24-25 0.06151673008268688 2403 26-27 -0.03340681817611113 2403 28-29 0.12586640106625424 2403 30-31 0.09461229103573743 2403 32-33 0.08142578995611416 2403 34-35 -0.023064090504114176 2403 36-37 0.2008610893072742 2403 38-39 0.11581233857896933 2403 40-41 0.06614777490343471 2403 42-43 0.058165479427081834 2403 44-45 0.14857704661845617 2403 46-47 0.0433946980149571 2403 48-49 0.0519910307967848 2403 50-51 0.086127199562398 2403 52-53 0.1742415357427518 2403 54-55 0.06417769919045213 2403 56-57 0.060986321885224015 2403 58-59 0.20761162367656283 2403 60-61 0.19023521150721479 2403 62-63 0.1461453079372106 2403 64-65 0.024337614523908258 2403 66-67 0.19482126968772917 2403 68-69 0.2033822443810962 2403 70-71 0.13550152218167 2403 72-73 0.052707048105119725 2403 74-75 0.04904248845247139 2403 76-77 0.34981250407172126 2403 78-79 0.1710644576845084 2403 80-81 0.19542531012249498 2403 82-83 -0.035048533574695284 2403 84-85 0.13157565870204735 2403 86-87 0.15733210596033587 2403 88-89 0.3717990448747841 2403 90-91 0.8273279671717191 2404 1 0.009132824884268587 2404 2 -0.08681761068723404 2404 3 -0.023688638356013314 2404 4 0.0521213411750594 2404 5 -0.019377102778278754 2404 6 -0.035627400878944115 2404 7 -0.013031780465823317 2404 8 -0.052311301730952664 2404 9 0.036734405512788726 2404 10-11 0.026964679184771967 2404 12-13 -0.04399196135671701 2404 14-15 0.0894415182991466 2404 16-17 -2.1420867666677168E-4 2404 18-19 -0.0145612223398075 2404 20-21 0.054161978171066494 2404 22-23 0.07100406180119023 2404 24-25 0.05406919137726618 2404 26-27 0.11900558335612743 2404 28-29 -0.0044275401454925145 2404 30-31 0.0978542758676113 2404 32-33 0.0240796239023382 2404 34-35 0.08373233933210145 2404 36-37 0.0982452039628825 2404 38-39 0.19205595394557662 2404 40-41 0.08589512731530391 2404 42-43 -0.04777449058117611 2404 44-45 -0.01737528296553137 2404 46-47 0.01358040877688893 2404 48-49 -0.1294782361225444 2404 50-51 0.023067099692234194 2404 52-53 0.08237295547530721 2404 54-55 0.05316906109227659 2404 56-57 -0.014212674067195508 2404 58-59 0.009413869319146784 2404 60-61 0.05391710378631842 2404 62-63 0.2436489627235261 2404 64-65 0.022663448024381694 2404 66-67 0.16053217375452533 2404 68-69 0.03920763930231175 2404 70-71 0.17333125513469838 2404 72-73 -0.07067389393112933 2404 74-75 -0.027227556015930077 2404 76-77 -0.1348235421275774 2404 78-79 -0.31767581385687294 2404 80-81 -0.30466713793862255 2404 82-83 -0.11022807375809407 2404 84-85 0.4583432307526394 2404 86-87 0.40895175068342837 2404 88-89 -0.2472485741728363 2404 90-91 0.8273279671717191 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 16.0 31 263.0 32 1710.0 33 2909.0 34 4000.0 35 4791.0 36 6693.0 37 9850.0 38 25870.0 39 153860.0 40 162959.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.057797764137714 9.522123988726836 31.498360242517855 32.9217180046176 2 27.64902926812261 15.761821366024517 27.909178438193116 28.67997092765976 3 28.242979075997326 17.878049238310528 20.41880183738647 33.46016984830567 4 27.00947385639318 18.811759058889148 18.922774528653523 35.25599255606415 5 27.863542144314746 19.276200589400972 19.13193410936901 33.72832315691527 6 29.283413913402573 21.718540924217194 22.186468447740943 26.811576714639294 7 26.58847316187611 20.886193054293003 22.06740837871828 30.45792540511261 8 25.64028306263257 22.070089911804377 22.39777325492531 29.89185377063775 9 26.440184382214998 22.100927542294478 22.636429699587847 28.82245837590267 10-11 25.698338253946545 20.83779138208897 22.582396807903013 30.881473556061472 12-13 26.566886820533036 20.57969382255223 22.22320545102046 30.630213905894276 14-15 26.91119566878776 20.299875845018114 22.129619946315707 30.65930853987842 16-17 26.77752124444587 19.955969226726303 21.82124364141467 31.445265887413154 18-19 27.072355807262127 20.335674311717494 21.716932004365535 30.875037876654844 20-21 27.13885782779731 20.466935356281894 21.704731028823797 30.689475787097 22-23 26.7531192933624 19.962941212750156 21.862807404249157 31.421132089638288 24-25 27.115126259985363 20.433952499322913 21.781959181703364 30.668962058988363 26-27 27.126254622292656 20.56883361355354 21.859053257928622 30.445858506225175 28-29 27.053853228968066 20.100503860066876 21.91094092314458 30.93470198782048 30-31 27.374749606476577 20.485475164448545 21.820505050775918 30.319270178298957 32-33 27.28388811717432 20.504126565480863 22.014878534272633 30.197106783072186 34-35 27.088973031628367 20.041131120196994 22.27686223377386 30.593033614400785 36-37 27.36133859552633 20.416029542418876 22.215439661059975 30.00719220099482 38-39 27.53456717446552 20.54982028419506 22.099540061548474 29.816072479790947 40-41 27.066937462977066 20.187018701870187 22.374714394516374 30.37132944063637 42-43 27.348127209502398 20.481590191074513 22.480353954150793 29.689928645272296 44-45 27.557984077057263 20.477067353310424 22.417857912088127 29.54709065754419 46-47 27.101681009945416 20.35001506881425 22.542946120617486 30.005357800622846 48-49 27.428422105071803 20.510361752408652 22.60748045809853 29.453735684421012 50-51 27.61930270970046 20.642679642277876 22.573893030569074 29.164124617452593 52-53 27.29964103430056 20.287009145727 22.871671124232638 29.541678695739805 54-55 27.645409213733735 20.51584164665358 22.733461777763083 29.1052873618496 56-57 27.750414334302885 20.654814597879405 22.61599696487643 28.978774102941273 58-59 27.42147542137172 20.401391054438523 22.812033583816955 29.3650999403728 60-61 27.87498496617909 20.707688607059467 22.613355009683854 28.80397141707759 62-63 27.994541571447932 20.614447077076935 22.60200672022282 28.789004631252308 64-65 27.47365238653421 20.65854447668097 22.520248014978492 29.34755512180633 66-67 27.56850043448934 20.828750432970146 22.711335006471764 28.891414126068753 68-69 27.95255214088881 20.748646644938383 22.348892836660188 28.949908377512624 70-71 27.6532173828552 20.495380604205636 22.977141885490806 28.87426012744836 72-73 28.002856462746966 20.68079028802666 22.55272554153773 28.763627707688645 74-75 28.20892485758206 20.813364680350848 22.41726195858577 28.56044850348133 76-77 28.08224766774553 20.5251992275682 22.289009165828052 29.103543938858216 78-79 27.757525083612038 21.10200668896321 22.285953177257525 28.854515050167223 80-81 28.79486369855805 20.890432586043577 21.91979791600884 28.394905799389537 82-83 28.58700689730378 20.71917341402906 21.790572776096617 28.90324691257054 84-85 28.664782113880833 20.378868534087154 21.56466830647234 29.391681045559675 86-87 29.08400620287685 19.72621784931287 21.651248596331747 29.538527351478532 88-89 29.038598999285202 19.111865618298786 22.122944960686205 29.72659042172981 90-91 26.882914907798266 20.262163963563655 24.99444567873806 27.860475449900022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.0 14 3.0 15 2.5 16 3.0 17 6.0 18 14.0 19 18.5 20 17.5 21 25.5 22 38.5 23 59.0 24 100.0 25 145.0 26 185.0 27 244.5 28 343.0 29 471.5 30 619.5 31 798.5 32 1010.0 33 1236.0 34 1591.5 35 2054.0 36 2550.5 37 3083.0 38 3711.5 39 4510.0 40 5392.0 41 6396.0 42 7460.0 43 8660.5 44 10047.0 45 11506.5 46 13174.0 47 15034.0 48 16858.5 49 18626.0 50 20519.5 51 22559.0 52 24245.5 53 25635.5 54 27007.5 55 28118.5 56 28942.5 57 29582.5 58 30071.5 59 30274.0 60 30174.5 61 29934.5 62 29292.5 63 28350.5 64 27340.5 65 25974.0 66 24261.0 67 22568.0 68 20654.0 69 18378.0 70 16040.5 71 13902.0 72 11924.5 73 9827.5 74 7962.0 75 6553.5 76 5307.0 77 4068.0 78 3092.5 79 2343.0 80 1729.5 81 1264.0 82 935.0 83 688.0 84 491.0 85 361.0 86 255.0 87 185.0 88 136.5 89 90.5 90 60.5 91 44.5 92 32.0 93 23.5 94 18.0 95 12.5 96 7.0 97 4.5 98 4.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0155528918993567 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.07221701451992424 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.076737035281536E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6815.0 32-33 14787.0 34-35 16333.0 36-37 16797.0 38-39 18107.0 40-41 18760.0 42-43 18849.0 44-45 18920.0 46-47 18887.0 48-49 18455.0 50-51 18177.0 52-53 17534.0 54-55 16293.0 56-57 15467.0 58-59 14701.0 60-61 13780.0 62-63 12893.0 64-65 12294.0 66-67 10975.0 68-69 9988.0 70-71 9411.0 72-73 8515.0 74-75 7643.0 76-77 6991.0 78-79 6331.0 80-81 5628.0 82-83 4834.0 84-85 4439.0 86-87 3822.0 88-89 3493.0 90-91 3002.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.81515796172631 #Duplication Level Percentage of deduplicated Percentage of total 1 99.82325921924665 99.63874387223457 2 0.16942710486305365 0.33822786469807337 3 0.006183795757581314 0.018517096510380956 4 0.001129880132713164 0.00451116655698323 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010726132344383932 0.0 0.0 0.0 0.0 2 0.0010726132344383932 0.0 0.0 0.0 0.0 3 0.0010726132344383932 0.0 0.0 0.0 0.0 4 0.0010726132344383932 0.0 0.0 0.0 0.0 5 0.0013407665430479913 0.0 0.0 0.0 2.681533086095983E-4 6 0.0013407665430479913 0.0 0.0 0.0 2.681533086095983E-4 7 0.0013407665430479913 0.0 0.0 0.0 5.363066172191966E-4 8 0.004022299629143974 0.0 0.0 0.0 5.363066172191966E-4 9 0.004290452937753573 0.0 2.681533086095983E-4 0.0 5.363066172191966E-4 10-11 0.004290452937753573 0.0 2.681533086095983E-4 0.0 5.363066172191966E-4 12-13 0.004290452937753573 0.0 2.681533086095983E-4 0.0 5.363066172191966E-4 14-15 0.004424529592058372 1.3407665430479915E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 16-17 0.004558606246363171 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 18-19 0.004558606246363171 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 20-21 0.004558606246363171 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 22-23 0.004558606246363171 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 24-25 0.004558606246363171 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 26-27 0.005094912863582367 2.681533086095983E-4 2.681533086095983E-4 0.0 5.363066172191966E-4 28-29 0.005094912863582367 2.681533086095983E-4 2.681533086095983E-4 0.0 6.703832715239957E-4 30-31 0.005094912863582367 2.681533086095983E-4 2.681533086095983E-4 0.0 8.044599258287948E-4 32-33 0.005363066172191965 2.681533086095983E-4 2.681533086095983E-4 0.0 8.044599258287948E-4 34-35 0.005765296135106363 2.681533086095983E-4 2.681533086095983E-4 0.0 8.044599258287948E-4 36-37 0.00616752609802076 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0010726132344383932 38-39 0.00616752609802076 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0010726132344383932 40-41 0.006435679406630358 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0010726132344383932 42-43 0.006435679406630358 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0012066898887431923 44-45 0.006435679406630358 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0013407665430479913 46-47 0.0065697560609351575 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0013407665430479913 48-49 0.006703832715239957 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0013407665430479913 50-51 0.006703832715239957 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0014748431973527903 52-53 0.006703832715239957 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0016089198516575896 54-55 0.006703832715239957 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0016089198516575896 56-57 0.0069719860238495555 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0016089198516575896 58-59 0.0072401393324591536 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0.0016089198516575896 80 0.008044599258287948 2.681533086095983E-4 2.681533086095983E-4 0.0 0.0016089198516575896 >>END_MODULE