##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-22B_S5_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 907420 Sequences flagged as poor quality 0 Sequence length 61 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.36946507681118 40.0 40.0 40.0 40.0 40.0 2 39.212426439796346 40.0 40.0 40.0 40.0 40.0 3 39.18145511450045 40.0 40.0 40.0 40.0 40.0 4 39.038284366665934 40.0 40.0 40.0 40.0 40.0 5 39.04780145908179 40.0 40.0 40.0 40.0 40.0 6 39.21687862290891 40.0 40.0 40.0 40.0 40.0 7 39.20092129333715 40.0 40.0 40.0 40.0 40.0 8 39.210438385753015 40.0 40.0 40.0 40.0 40.0 9 39.242904057657974 40.0 40.0 40.0 40.0 40.0 10 39.26221595292147 40.0 40.0 40.0 40.0 40.0 11 39.237830332150494 40.0 40.0 40.0 40.0 40.0 12 39.24345947852152 40.0 40.0 40.0 40.0 40.0 13 39.13555354742016 40.0 40.0 40.0 40.0 40.0 14 39.27014612858434 40.0 40.0 40.0 40.0 40.0 15 39.23026602896123 40.0 40.0 40.0 40.0 40.0 16 39.24229573956933 40.0 40.0 40.0 40.0 40.0 17 39.274699698045005 40.0 40.0 40.0 40.0 40.0 18 39.231050671133545 40.0 40.0 40.0 40.0 40.0 19 39.27961473187719 40.0 40.0 40.0 40.0 40.0 20 38.886341495669036 40.0 40.0 40.0 40.0 40.0 21 39.12516365079015 40.0 40.0 40.0 40.0 40.0 22 39.11503824028564 40.0 40.0 40.0 40.0 40.0 23 39.23991536443984 40.0 40.0 40.0 40.0 40.0 24 39.259315421745164 40.0 40.0 40.0 40.0 40.0 25 38.98320953913293 40.0 40.0 40.0 40.0 40.0 26 39.20871040973309 40.0 40.0 40.0 40.0 40.0 27 39.21315377664147 40.0 40.0 40.0 40.0 40.0 28 38.91977695003416 40.0 40.0 40.0 40.0 40.0 29 39.16035352978775 40.0 40.0 40.0 40.0 40.0 30 38.76435167838487 40.0 40.0 40.0 40.0 40.0 31 38.943836371250356 40.0 40.0 40.0 40.0 40.0 32 39.043715148442836 40.0 40.0 40.0 40.0 40.0 33 39.0325494258447 40.0 40.0 40.0 40.0 40.0 34 38.848475898701814 40.0 40.0 40.0 40.0 40.0 35 38.79397412444072 40.0 40.0 40.0 40.0 40.0 36 38.95487866698993 40.0 40.0 40.0 40.0 40.0 37 38.81359017874854 40.0 40.0 40.0 40.0 40.0 38 38.7485089594675 40.0 40.0 40.0 40.0 40.0 39 38.73262656763131 40.0 40.0 40.0 40.0 40.0 40 38.464591919948866 40.0 40.0 40.0 40.0 40.0 41 38.52527385334244 40.0 40.0 40.0 40.0 40.0 42 38.28713936214763 40.0 40.0 40.0 40.0 40.0 43 38.52421150073836 40.0 40.0 40.0 40.0 40.0 44 38.0280663860175 40.0 40.0 40.0 40.0 40.0 45 38.28554803729254 40.0 40.0 40.0 40.0 40.0 46 37.87801018271583 40.0 40.0 40.0 24.0 40.0 47 37.34858830530515 40.0 40.0 40.0 24.0 40.0 48 37.64988208326905 40.0 40.0 40.0 24.0 40.0 49 37.288259020078904 40.0 40.0 40.0 24.0 40.0 50 37.4372484626744 40.0 40.0 40.0 24.0 40.0 51 36.98834497806969 40.0 40.0 40.0 24.0 40.0 52 36.95157699852329 40.0 40.0 40.0 24.0 40.0 53 36.19389037050098 40.0 40.0 40.0 24.0 40.0 54 35.46125939476758 40.0 40.0 40.0 12.0 40.0 55 35.34526900443014 40.0 40.0 40.0 12.0 40.0 56 35.07315245421084 40.0 40.0 40.0 12.0 40.0 57 35.05836327169337 40.0 40.0 40.0 12.0 40.0 58 34.946428335280245 40.0 40.0 40.0 12.0 40.0 59 34.435233960018515 40.0 24.0 40.0 12.0 40.0 60 33.673981177404066 40.0 24.0 40.0 12.0 40.0 61 33.48853673051068 40.0 24.0 40.0 12.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.024973279789548997 1101 2 0.039866656399787814 1101 3 -0.01362629788766867 1101 4 -0.026051586301775842 1101 5 -6.426598696407382E-5 1101 6 -0.021310992022570474 1101 7 0.037860079424966386 1101 8 0.0442117176089738 1101 9 0.01865977042466227 1101 10 0.07899792711916831 1101 11 0.018405699125523256 1101 12 -0.017151072178769766 1101 13 -0.015623408173922826 1101 14 0.06915146610130307 1101 15 0.07812639820545542 1101 16 -0.04939826151623805 1101 17 -0.0374818360711302 1101 18 0.08986395785787948 1101 19 -0.019159434966859124 1101 20 0.012528674128894579 1101 21 0.0870521970623841 1101 22 0.0061393388216473 1101 23 0.11891252404605979 1101 24 0.10869020404101093 1101 25 0.034936591084480995 1101 26 0.1340285075501555 1101 27 0.04437505428349908 1101 28 0.10767642046385362 1101 29 -0.016820149324402678 1101 30 -0.08199542063113086 1101 31 -0.04342655933010775 1101 32 0.041663109237262574 1101 33 -0.05714388789963465 1101 34 0.03668922587323209 1101 35 0.1335480283147774 1101 36 0.025383610794030176 1101 37 0.06696025556937713 1101 38 -0.042114379532790736 1101 39 -0.1128583861250334 1101 40 -0.013483037972186196 1101 41 0.11281469726507964 1101 42 -0.11434203075069149 1101 43 0.0993200295539367 1101 44 0.11425570631130455 1101 45 0.07231774351764386 1101 46 -0.04651607427886972 1101 47 -0.01265089045089951 1101 48 0.4615556280097408 1101 49 0.271370761577721 1101 50 0.20927339746886986 1101 51 0.2681764539020648 1101 52 0.2788226057477061 1101 53 0.10848962025051634 1101 54 0.18292115936330333 1101 55 0.10014848275251609 1101 56 0.24569415736986855 1101 57 0.1467108068847267 1101 58 0.6240525821364145 1101 59 0.31066079558264903 1101 60 0.1978569375375514 1101 61 0.3947156554105007 1102 1 0.005179408718625211 1102 2 -0.08177651971340083 1102 3 -0.06049584052819057 1102 4 -0.25267943436949025 1102 5 -0.05973599461339063 1102 6 -0.05277502066303441 1102 7 -0.054636136002599756 1102 8 -0.024594791192981802 1102 9 -0.0751265429469754 1102 10 -0.15502352672290698 1102 11 -0.08934181612873004 1102 12 -0.0901748567030154 1102 13 -0.20298254467093813 1102 14 -0.07860354299793215 1102 15 -0.09760113426536066 1102 16 -0.10448645575863225 1102 17 -0.13833549585658744 1102 18 -0.1400134719942514 1102 19 -0.040659782249946375 1102 20 -0.08645031961157201 1102 21 -0.15289300871414468 1102 22 -0.13535023756527664 1102 23 -0.12201435132780603 1102 24 -0.10161070034077824 1102 25 -0.03733298974814403 1102 26 -0.1027350670620919 1102 27 -0.17875116821020498 1102 28 0.03822992284242588 1102 29 -0.08175636429413657 1102 30 -0.039788447138498384 1102 31 -0.04089551920972667 1102 32 -0.11522427044104688 1102 33 0.016429284617231588 1102 34 0.07253823814951943 1102 35 -0.04465705123503483 1102 36 -0.20687498227874102 1102 37 -0.05289692764850429 1102 38 -0.15967666308326045 1102 39 0.04594028546443951 1102 40 0.060096399495471076 1102 41 -0.05505417731482254 1102 42 -0.08048238131082996 1102 43 -0.08390038406534472 1102 44 -0.07023311888391959 1102 45 -0.008661564675165323 1102 46 -0.012226070911459885 1102 47 -0.1910029553847039 1102 48 0.19354247884186293 1102 49 -0.09275058810954562 1102 50 0.009527971334748031 1102 51 -0.18427636469326814 1102 52 -0.08055087507702297 1102 53 0.020402484423371448 1102 54 0.12363255657383831 1102 55 -0.013941572300744554 1102 56 -0.08145675384915307 1102 57 0.08336540749711929 1102 58 -0.02921070422785732 1102 59 -0.05561376950025476 1102 60 -0.102816363952968 1102 61 -0.1505953000005178 1103 1 -0.07578002380692084 1103 2 -0.0462280292078745 1103 3 -0.02850571891411846 1103 4 0.0471743852108375 1103 5 -0.1627536517288064 1103 6 2.352103015539342E-4 1103 7 -0.11779159488936841 1103 8 -0.06242784870634921 1103 9 -0.05672158025669205 1103 10 -0.11221702914416198 1103 11 0.01171757274364893 1103 12 0.031061306665648658 1103 13 0.092964727161025 1103 14 0.02131808108519806 1103 15 0.07212178885311005 1103 16 0.02409523505164657 1103 17 -0.04663650551582066 1103 18 0.02877859590157783 1103 19 -0.12322430979276078 1103 20 -0.05670766397960847 1103 21 0.07406252749021291 1103 22 -0.09732929665234735 1103 23 9.495356486866058E-6 1103 24 -0.11165889481103619 1103 25 0.09570225111302477 1103 26 0.0028454147541836505 1103 27 -0.008930735985629212 1103 28 -0.05481469316254817 1103 29 0.040792604600575544 1103 30 0.04465871963334678 1103 31 -0.17842093549334237 1103 32 -0.010685946924624545 1103 33 -0.11271439160211116 1103 34 -0.03238488959537733 1103 35 0.12578410968935572 1103 36 0.026405803159484265 1103 37 0.015622007728048004 1103 38 0.05250648137374725 1103 39 0.003767359248271873 1103 40 -0.01947626327962837 1103 41 0.20725673379956078 1103 42 0.15978814224529003 1103 43 0.07634512959419482 1103 44 0.30932179009905525 1103 45 0.07287983073484128 1103 46 0.1390471780241782 1103 47 0.1352501466237328 1103 48 0.33141559832821343 1103 49 0.27581528244283504 1103 50 0.22181908928973115 1103 51 0.19944812879746365 1103 52 0.30486028812217825 1103 53 0.3095764407875663 1103 54 0.11868004529220855 1103 55 0.26577495207992285 1103 56 0.3609054351636871 1103 57 0.44970854608455824 1103 58 0.3614226661708244 1103 59 0.21295865318840157 1103 60 0.6087109128519614 1103 61 0.3769541736821935 1104 1 -0.018729553172946112 1104 2 0.038980225044184635 1104 3 -0.15299995724084425 1104 4 0.015980352211599325 1104 5 -0.11950277190118186 1104 6 0.026458392544043363 1104 7 0.06188733623394427 1104 8 -0.05175737191832752 1104 9 -0.04158747276068908 1104 10 -0.03678595986561106 1104 11 0.02855954105442038 1104 12 -0.02483236285338819 1104 13 -0.019818196210678707 1104 14 -0.04953433732561763 1104 15 -0.07050320924658138 1104 16 0.012124181948983903 1104 17 0.008625967306841176 1104 18 -0.001057822097237704 1104 19 -0.033910684346722064 1104 20 -0.05508559689056369 1104 21 -0.11059668227179031 1104 22 -0.03661043715265322 1104 23 -0.09893801125746648 1104 24 -0.08031071594196248 1104 25 0.03142955151326987 1104 26 -0.1265192673865485 1104 27 -0.03507660959615322 1104 28 -0.03233579423545763 1104 29 0.03393712024254825 1104 30 -0.06277953746239717 1104 31 0.019823852154807753 1104 32 -0.020144940882410367 1104 33 -0.006595330887847695 1104 34 0.0201340695805996 1104 35 -0.12013229163153483 1104 36 -0.0688403170019356 1104 37 0.014554300097508133 1104 38 -0.163709263448375 1104 39 -0.009153081566488197 1104 40 -0.09763670438194794 1104 41 -0.27965612864799283 1104 42 -0.1953554567708693 1104 43 -0.07393813701133922 1104 44 -0.19120681945688034 1104 45 -0.008564341649737628 1104 46 -0.14192350255930108 1104 47 -0.21398829853468726 1104 48 -0.5229720613285522 1104 49 -0.23016264287867472 1104 50 -0.34532457713578424 1104 51 -0.21517173487772112 1104 52 -0.2705007202086449 1104 53 -0.17597126435401123 1104 54 -0.1319979078061948 1104 55 0.006980535413035227 1104 56 -0.01619711509016497 1104 57 -0.15709539132892303 1104 58 -0.35571435949033514 1104 59 -0.4644040241132288 1104 60 -0.4351663318825345 1104 61 -0.43548271106748615 1201 1 0.03244517026663374 1201 2 -0.06946472466193399 1201 3 0.01989614671892781 1201 4 0.0516977509264791 1201 5 -0.029164502581593865 1201 6 0.10251371981384239 1201 7 0.09650089653966631 1201 8 -0.00819305952105509 1201 9 0.09539992600081604 1201 10 -0.06353586717164461 1201 11 0.06734884362630567 1201 12 -0.03209196563177841 1201 13 -0.06543809677196322 1201 14 0.06549107714286606 1201 15 -0.017251614796720105 1201 16 -0.007483776065939196 1201 17 -0.03160817748084099 1201 18 -0.09988914274687488 1201 19 0.02147636872397385 1201 20 -0.027867466966441157 1201 21 -0.030671224843047185 1201 22 0.0382348766097067 1201 23 -0.05691993351218372 1201 24 -0.010198324578631457 1201 25 0.04189258135600937 1201 26 0.051215455804332066 1201 27 0.10026985780408637 1201 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18 0.10264736968203891 2403 19 0.02166860966595152 2403 20 -0.045639074047315376 2403 21 0.05731104627008676 2403 22 0.09791500904236017 2403 23 0.04095206606061197 2403 24 0.0397629602316627 2403 25 -0.01697586709939003 2403 26 0.007320440718757482 2403 27 -0.019581689468495256 2403 28 0.07199745672878777 2403 29 0.07450658850590486 2403 30 -0.02293228796354896 2403 31 -0.006961343839464007 2403 32 0.09359292944727571 2403 33 0.03240448239517946 2403 34 0.022484608194346833 2403 35 -0.024530739477420127 2403 36 0.009519679473243059 2403 37 -0.08449861968136219 2403 38 0.08702685124883658 2403 39 -0.08612582643532107 2403 40 -0.021536537815620704 2403 41 0.04437607047733394 2403 42 0.16469549292832397 2403 43 0.08602567810311967 2403 44 0.0061299680376478705 2403 45 0.19324921622050795 2403 46 0.1856266167731775 2403 47 -0.13343354578677236 2403 48 -0.20877731984527514 2403 49 0.16251829849527866 2403 50 0.22572763767870185 2403 51 0.02767518740427022 2403 52 0.03890420727060473 2403 53 0.11312280094269767 2403 54 0.3800768874367719 2403 55 0.010106534857193594 2403 56 0.16889695513636838 2403 57 -0.333522836381448 2403 58 -0.30570872100766167 2403 59 0.02231226530916075 2403 60 -0.07680039497315505 2403 61 0.01200699330964028 2404 1 0.008132896066065598 2404 2 0.03790654216149392 2404 3 0.05310057984318206 2404 4 -0.06585679537248978 2404 5 -0.09273153476427609 2404 6 0.047461434564681326 2404 7 0.014487608899905524 2404 8 0.01551300889219931 2404 9 -0.10554995935784461 2404 10 0.07276608197551582 2404 11 9.086893371588189E-4 2404 12 -0.003859886590369399 2404 13 -0.06476295952013089 2404 14 -0.04093225982444437 2404 15 0.06991491091080348 2404 16 0.015214719663731557 2404 17 -0.08770274558366253 2404 18 -0.06581744424625668 2404 19 -0.040618170046307966 2404 20 -0.20482675829232022 2404 21 -0.07194895020248993 2404 22 -0.08531964035672246 2404 23 -0.1039571027267101 2404 24 -0.0688893111785518 2404 25 0.009391640270408175 2404 26 -0.06634984575860159 2404 27 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-0.35839538518314384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 19.0 15 110.0 16 307.0 17 585.0 18 881.0 19 989.0 20 1069.0 21 1107.0 22 1276.0 23 1510.0 24 1982.0 25 2352.0 26 2857.0 27 4135.0 28 6161.0 29 6233.0 30 6667.0 31 8236.0 32 13039.0 33 15188.0 34 20235.0 35 30817.0 36 40223.0 37 61196.0 38 117838.0 39 316564.0 40 245837.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.073424685311757 30.91626019664847 18.587049237055957 21.423265880983816 2 29.261532697097266 18.729915584844946 21.396045932423796 30.612505785634 3 30.411386127702716 19.093914615062484 19.10350223711181 31.391197020122984 4 30.826849749840207 20.099623107271164 20.029975094223182 29.04355204866545 5 28.11641797624033 20.752573229595995 20.902228295607326 30.228780498556347 6 27.25915232196778 20.198584999228583 23.040708822816335 29.501553855987307 7 29.32368693658945 22.432500936721695 22.35954684710498 25.88426527958387 8 27.26444204447775 23.688259020078906 23.855987304665977 25.19131163077737 9 27.049767472614665 23.358973793833066 23.70159352890613 25.88966520464614 10 29.584536377862513 23.003901170351103 22.47063101981442 24.940931431971965 11 28.01932952767186 23.716140265808555 22.65015097749664 25.614379229022944 12 28.328117079191557 22.11765224482599 23.01337858984814 26.540852086134315 13 29.39388596239889 22.86780101827158 21.646426131229198 26.09188688810033 14 29.54541447179917 22.667011967997176 21.965131912455092 25.822441647748562 15 28.508849264948978 22.107183002358337 22.8923761874325 26.49159154526019 16 29.500231425359814 22.684534173811464 21.393841881377973 26.421392519450748 17 29.9702453108814 22.80101827158317 21.47109387053404 25.757642547001385 18 28.338806726763792 22.055167397676932 22.884551806219832 26.721474069339447 19 30.53282934032752 22.914857508099885 21.00978598664345 25.54252716492914 20 28.891803134160586 22.80090806903088 22.523969055123317 25.78331974168522 21 28.672389852548985 23.189923078618502 21.83795816711115 26.299728901721366 22 30.530294681624824 23.066165612395583 21.02256948270922 25.38097022327037 23 30.01818342112803 23.23764078376055 21.423155760287408 25.321020034824006 24 29.489541777787576 22.53509951290472 21.71574353662031 26.2596151726874 25 29.828965638844195 24.17568490886249 20.853959577703822 25.1413898745895 26 29.177227744594564 24.400167507879484 21.298847281303036 25.12375746622292 27 28.92431288708647 24.047629543100218 21.433735205307354 25.594322364505963 28 29.838883868550397 25.02071807983073 20.626501509774968 24.51389654184391 29 29.59820149434661 25.06149302417844 20.796103237750987 24.544202243723966 30 29.016883031010998 24.742566837847964 21.07524630270437 25.16530382843667 31 30.27782063432589 24.751272839478965 21.12792312269952 23.842983403495623 32 30.644244120693838 24.869850785744198 20.538339467942077 23.94756562561989 33 29.60467879049695 24.59715190640656 21.386489518565945 24.411679784530545 34 30.43706332238655 25.49910735932644 19.982477794185712 24.0813515241013 35 30.62683211743184 25.83698838465099 20.069978620704855 23.466200877212316 36 30.110312754843406 24.859381543276545 20.334685151308104 24.695620550571952 37 31.0104472019572 25.792135945868505 19.858389720305922 23.339027131868374 38 30.99204337572458 25.739128518216482 19.69815520927465 23.570672896784288 39 31.448612549866656 25.614379229022944 19.731656785171143 23.205351435939257 40 31.794428158956162 25.812192810385486 19.75876661303476 22.634612417623593 41 32.02199642943731 26.06345462960922 19.70168169094796 22.212867250005512 42 32.14762733904917 25.679949747636154 19.661347556809417 22.511075356505255 43 32.57510303938639 26.13651892177823 19.700138855215886 21.588239183619493 44 32.261686980670476 26.281876088250204 20.05069317405391 21.405743757025412 45 32.62403297260364 25.89109783782592 19.712922351281655 21.771946838288773 46 33.59183178682418 25.830596636618104 18.25802825593441 22.319543320623307 47 33.53684071323092 25.77086685327632 18.419364792488594 22.272927641004163 48 33.5172246589231 27.042604306715745 18.390932533997486 21.049238500363668 49 34.74521169910295 26.169359282360983 17.693129972890173 21.392299045645895 50 33.7068832514161 27.427652024420883 18.41616891847215 20.44929580569086 51 33.75713561526085 27.39910956337749 18.355998324921206 20.487756496440458 52 34.17270943995063 27.88411099601067 18.090410173899627 19.852769390139073 53 34.83623900729541 25.978047651583612 17.305768001586916 21.879945339534064 54 33.89488880562474 25.575918538273346 17.315796433845406 23.213396222256506 55 35.07240307685526 26.12990676864076 18.054373939300433 20.743316215203546 56 33.66996539639858 27.21595292146966 19.854973441184896 19.25910824094686 57 34.26869586299618 28.427850389015006 18.599986775693726 18.70346697229508 58 33.38343876044169 29.46551762138811 18.97445504837892 18.176588569791278 59 32.98461572370016 29.933658063520753 18.183310925481035 18.898415287298054 60 32.433602962244606 26.72169447444403 22.05891428445483 18.78578827885654 61 33.217914526900444 29.29371184236627 18.519538912521213 18.968834718212076 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3.0 1 1.5 2 2.5 3 5.0 4 5.0 5 5.0 6 5.0 7 4.5 8 4.0 9 4.0 10 4.0 11 9.5 12 16.0 13 17.0 14 16.5 15 16.0 16 31.0 17 47.0 18 48.0 19 72.0 20 121.0 21 146.0 22 218.5 23 291.0 24 362.5 25 594.0 26 754.0 27 979.5 28 1205.0 29 1491.0 30 2219.0 31 2661.0 32 3247.5 33 3834.0 34 4539.5 35 6115.5 36 6986.0 37 8311.5 38 9637.0 39 11246.5 40 14994.0 41 17132.0 42 19462.5 43 24621.5 44 27450.0 45 30930.5 46 34411.0 47 38463.0 48 46574.0 49 50633.0 50 55368.5 51 60104.0 52 63755.0 53 69894.0 54 72382.0 55 72734.5 56 73087.0 57 72754.0 58 70057.0 59 67693.0 60 64276.5 61 56173.5 62 51487.0 63 46718.0 64 41949.0 65 37076.5 66 27804.0 67 23404.0 68 19807.5 69 16211.0 70 13448.0 71 8819.5 72 6954.0 73 5576.0 74 4198.0 75 3310.0 76 1942.0 77 1462.0 78 1131.0 79 800.0 80 634.0 81 355.0 82 242.0 83 184.5 84 106.5 85 86.0 86 65.5 87 45.0 88 39.0 89 33.0 90 33.0 91 34.0 92 35.0 93 44.0 94 48.0 95 43.0 96 45.0 97 47.0 98 65.5 99 227.5 100 371.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001542835732075555 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 6.612153137466664E-4 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 60 0.0 61 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 907420.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.14643044418759 #Duplication Level Percentage of deduplicated Percentage of total 1 85.09873824638473 69.9055758224477 2 11.3839480031533 18.703013856425617 3 2.3629004336842936 5.823115083665625 4 0.6102289399150382 2.0051251667104406 5 0.2095870629406275 0.8608414543926912 6 0.10093204492033539 0.49747243245827694 7 0.05963567192338662 0.34292003029528084 8 0.04182906262612754 0.2748886546850202 9 0.03299007182307962 0.2439014976327027 >10 0.09663568677493387 1.128582230790367 >50 0.0014304310301026112 0.06850530021868702 >100 0.001144344824082089 0.14605847027757796 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.8415066892949241 0.0 0.0 0.0 0.0 2 0.8438209428930374 0.0 0.0 0.0 2.2040510458222213E-4 3 0.8449229684159485 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 4 0.8458045888342774 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 5 0.8467964118048974 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 6 0.8497718807167574 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 7 0.8535187674946552 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 8 0.8628859844393996 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 9 0.8660818584558418 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 10 0.8696083401291574 1.1020255229111106E-4 0.0 0.0 2.2040510458222213E-4 11 0.8777633289986996 2.2040510458222213E-4 0.0 0.0 2.2040510458222213E-4 12 0.8975997884110996 2.2040510458222213E-4 0.0 1.1020255229111106E-4 2.2040510458222213E-4 13 0.9259218443499151 2.2040510458222213E-4 0.0 1.1020255229111106E-4 4.4081020916444426E-4 14 0.9600846355601595 2.2040510458222213E-4 0.0 1.1020255229111106E-4 4.4081020916444426E-4 15 1.008022745806793 2.2040510458222213E-4 0.0 1.1020255229111106E-4 4.4081020916444426E-4 16 1.0668709087302461 2.2040510458222213E-4 0.0 1.1020255229111106E-4 4.4081020916444426E-4 17 1.1561349760860462 2.2040510458222213E-4 0.0 1.1020255229111106E-4 4.4081020916444426E-4 18 1.2946595843159727 2.2040510458222213E-4 0.0 1.1020255229111106E-4 5.510127614555553E-4 19 1.4669061735469793 2.2040510458222213E-4 0.0 1.1020255229111106E-4 5.510127614555553E-4 20 1.6963478874170725 2.2040510458222213E-4 0.0 1.1020255229111106E-4 5.510127614555553E-4 21 2.016265896718168 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 22 2.3904035617464903 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 23 2.8462013180225254 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 24 3.416168918472152 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 25 4.050164201802914 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 26 4.785545833241498 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 27 5.641599259438848 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 28 6.553415177095501 2.2040510458222213E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 29 7.56595622754623 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 30 8.740935840074057 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 31 9.971016728747438 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 32 11.305239029335919 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 33 12.767737100791255 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 34 14.239051376429877 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 35 15.803597011306781 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 36 17.46853717132089 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 37 19.15904432346653 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 38 20.92470961627471 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 39 22.747129223512818 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 40 24.633466311079765 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 41 26.575786295210598 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 42 28.58081153159507 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 43 30.572502259152323 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 44 32.59824557536753 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 45 34.615833902713185 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 46 36.59033303211302 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 47 38.57254634017324 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 48 40.64666857684424 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 49 42.59791496771065 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 50 44.57142227413987 4.4081020916444426E-4 2.2040510458222213E-4 1.1020255229111106E-4 5.510127614555553E-4 >>END_MODULE