##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-22B_S5_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907420
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.45187013731238	40.0	40.0	40.0	40.0	40.0
2	39.176088250203875	40.0	40.0	40.0	40.0	40.0
3	39.13070022701726	40.0	40.0	40.0	40.0	40.0
4	39.00762160851645	40.0	40.0	40.0	40.0	40.0
5	39.04799541557382	40.0	40.0	40.0	40.0	40.0
6	39.1799519516872	40.0	40.0	40.0	40.0	40.0
7	39.189909854312226	40.0	40.0	40.0	40.0	40.0
8	39.214198496837184	40.0	40.0	40.0	40.0	40.0
9	39.210676423265966	40.0	40.0	40.0	40.0	40.0
10	39.22520993586212	40.0	40.0	40.0	40.0	40.0
11	39.21642899649556	40.0	40.0	40.0	40.0	40.0
12	39.227215622313814	40.0	40.0	40.0	40.0	40.0
13	39.040708822816335	40.0	40.0	40.0	40.0	40.0
14	39.219369200590684	40.0	40.0	40.0	40.0	40.0
15	39.121707698750306	40.0	40.0	40.0	40.0	40.0
16	39.30053558440414	40.0	40.0	40.0	40.0	40.0
17	39.24920764364903	40.0	40.0	40.0	40.0	40.0
18	39.33847171100483	40.0	40.0	40.0	40.0	40.0
19	39.278415728108264	40.0	40.0	40.0	40.0	40.0
20	39.28818408234335	40.0	40.0	40.0	40.0	40.0
21	39.267827466884135	40.0	40.0	40.0	40.0	40.0
22	39.329188247999824	40.0	40.0	40.0	40.0	40.0
23	39.32047783826673	40.0	40.0	40.0	40.0	40.0
24	39.320125190099404	40.0	40.0	40.0	40.0	40.0
25	39.27584139648674	40.0	40.0	40.0	40.0	40.0
26	39.23640651517489	40.0	40.0	40.0	40.0	40.0
27	39.25637962575213	40.0	40.0	40.0	40.0	40.0
28	39.26264794692645	40.0	40.0	40.0	40.0	40.0
29	39.26910581649071	40.0	40.0	40.0	40.0	40.0
30	39.262440766128144	40.0	40.0	40.0	40.0	40.0
31	39.20331048467082	40.0	40.0	40.0	40.0	40.0
32	39.23032333428842	40.0	40.0	40.0	40.0	40.0
33	39.2328668091953	40.0	40.0	40.0	40.0	40.0
34	39.22822948579489	40.0	40.0	40.0	40.0	40.0
35	39.241995988627096	40.0	40.0	40.0	40.0	40.0
36	39.20541755747063	40.0	40.0	40.0	40.0	40.0
37	39.21334332503141	40.0	40.0	40.0	40.0	40.0
38	39.21454673690243	40.0	40.0	40.0	40.0	40.0
39	37.989120804037825	40.0	40.0	40.0	40.0	40.0
40	39.20883383659166	40.0	40.0	40.0	40.0	40.0
41	39.217412003262	40.0	40.0	40.0	40.0	40.0
42	39.18196204624099	40.0	40.0	40.0	40.0	40.0
43	39.20560269775848	40.0	40.0	40.0	40.0	40.0
44	39.16455445108109	40.0	40.0	40.0	40.0	40.0
45	39.05141610279694	40.0	40.0	40.0	40.0	40.0
46	39.03954067576205	40.0	40.0	40.0	40.0	40.0
47	39.05556853496727	40.0	40.0	40.0	40.0	40.0
48	39.2015825086509	40.0	40.0	40.0	40.0	40.0
49	39.17350289832712	40.0	40.0	40.0	40.0	40.0
50	39.16879063719116	40.0	40.0	40.0	40.0	40.0
51	39.12748672059245	40.0	40.0	40.0	40.0	40.0
52	39.14189680633003	40.0	40.0	40.0	40.0	40.0
53	39.05524674351458	40.0	40.0	40.0	40.0	40.0
54	38.94343523396002	40.0	40.0	40.0	40.0	40.0
55	38.99965616803685	40.0	40.0	40.0	40.0	40.0
56	39.017791100041876	40.0	40.0	40.0	40.0	40.0
57	38.91745828833396	40.0	40.0	40.0	40.0	40.0
58	38.889171497211876	40.0	40.0	40.0	40.0	40.0
59	38.842264882854685	40.0	40.0	40.0	40.0	40.0
60	38.810314958894445	40.0	40.0	40.0	40.0	40.0
61	38.67869784664213	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.0029996121638760087
1101	2	0.003939311774836085
1101	3	0.059778030457096065
1101	4	-0.10061886087712679
1101	5	-0.017039712613062363
1101	6	0.032491269824191704
1101	7	0.07191363990351363
1101	8	-0.11090652818295865
1101	9	-0.11277056171499567
1101	10	0.00289962006625899
1101	11	0.02530578049118759
1101	12	0.020859275420612278
1101	13	-0.02915394813950911
1101	14	-0.11132198751359113
1101	15	-0.04504425949262725
1101	16	-0.009943923425183243
1101	17	0.027055809124703956
1101	18	-0.08400227316560205
1101	19	-0.060028893134152383
1101	20	-0.06027764623568288
1101	21	-0.10619886267340917
1101	22	0.00878137265387835
1101	23	-0.07350437171484714
1101	24	-0.01031036309129263
1101	25	9.404180486072278E-4
1101	26	-0.08655185748887817
1101	27	0.03904306137075508
1101	28	-0.09329798105097353
1101	29	-0.021665859685221278
1101	30	0.06463837290190355
1101	31	8.514438195277307E-4
1101	32	-0.04902983976206343
1101	33	0.03741065821730416
1101	34	-0.0018338134325119881
1101	35	-0.04009899119865423
1101	36	-0.10805628138226098
1101	37	-0.008640298594023932
1101	38	-0.07644200384850564
1101	39	1.1128222618006376
1101	40	-0.07773385898564555
1101	41	-0.10773902769837918
1101	42	0.010469162996464831
1101	43	-0.10709512832181645
1101	44	-0.059940426852520545
1101	45	-0.11900654199418881
1101	46	0.049179680263804926
1101	47	0.01511077779965575
1101	48	-0.06243350841457129
1101	49	-0.06023298180350878
1101	50	-0.08178779493280075
1101	51	-0.0672680321417829
1101	52	0.004499108002690377
1101	53	-0.05566162714536205
1101	54	0.06435023308718968
1101	55	-0.15600644429029842
1101	56	-0.003767674410688926
1101	57	0.1271747832177823
1101	58	0.041439095930520864
1101	59	-0.025113554015987916
1101	60	0.02254026536351006
1101	61	0.06964300954626168
1102	1	-0.02682184659472142
1102	2	-0.1513244818094961
1102	3	0.05753035888027824
1102	4	0.02432739006186324
1102	5	-0.20884744609242034
1102	6	-0.13512170561178038
1102	7	-0.17895764005605486
1102	8	-0.14257103524879966
1102	9	0.047427603497780524
1102	10	-0.05484587715096012
1102	11	-0.07549390433359093
1102	12	-0.1272311495056968
1102	13	-0.20148925071554658
1102	14	-0.13744056736972965
1102	15	-0.0726572710725506
1102	16	-0.024461742089798122
1102	17	-0.10706955856466038
1102	18	-0.09720300640580604
1102	19	-0.04690960425426027
1102	20	0.02929476484870719
1102	21	-0.11822901395389351
1102	22	-0.08617552267746476
1102	23	-0.05887908504816863
1102	24	-0.10124660577138656
1102	25	-0.07891939162941952
1102	26	-0.08757882749964097
1102	27	-0.10472599326624277
1102	28	-0.03999385202672556
1102	29	-0.048773819847362176
1102	30	-0.03314931634930929
1102	31	-0.07923583176421545
1102	32	-0.17505607183391447
1102	33	0.14660971437837134
1102	34	-0.04851058835632216
1102	35	0.04116609513417302
1102	36	-0.15643953251823461
1102	37	0.0016790392006811317
1102	38	-0.12330769113469131
1102	39	1.062979277129891
1102	40	-0.05370391734005153
1102	41	0.01488919144718892
1102	42	-0.10717649259147777
1102	43	-0.09570930313442716
1102	44	0.03902507314778347
1102	45	-0.08028047154602547
1102	46	0.042444858161296395
1102	47	-0.10563169115721394
1102	48	-0.030061543828409754
1102	49	-0.1433592404411641
1102	50	-0.08573037665878047
1102	51	0.0524831288319092
1102	52	-0.04099166354406947
1102	53	-0.1113088477662032
1102	54	-0.09973557505230701
1102	55	-0.03543553860129833
1102	56	-0.18583533708405042
1102	57	0.08973688303652239
1102	58	-0.04630539528017863
1102	59	0.08417272953688837
1102	60	0.09433756529019632
1102	61	0.14267643245634787
1103	1	0.05911078816910731
1103	2	0.13455723417477827
1103	3	0.02512736944134275
1103	4	0.0733516390306761
1103	5	0.10516231742303006
1103	6	-0.01350359193970263
1103	7	-0.0063127733337609016
1103	8	0.018316611548485184
1103	9	-0.04647842222012599
1103	10	-0.01450184124192333
1103	11	-0.14284225478274948
1103	12	-0.050038184573658384
1103	13	-0.06572898707945996
1103	14	-0.027185831964850138
1103	15	-0.07263491532626887
1103	16	-0.04573014292024169
1103	17	0.027029720688460657
1103	18	-0.09891916667319833
1103	19	0.03908676816916312
1103	20	-0.024038910955638926
1103	21	0.057811072277452524
1103	22	-0.05804818862755923
1103	23	-0.08851138891127874
1103	24	0.00840737840906769
1103	25	-0.11970816419311348
1103	26	-0.013669778999442883
1103	27	0.011233262672689648
1103	28	-0.011341395766393703
1103	29	-0.024248614873116026
1103	30	0.030886102763261647
1103	31	0.03590196941257773
1103	32	-0.013708943102507476
1103	33	0.007206415407488009
1103	34	0.043966096562385815
1103	35	-0.012233220946470169
1103	36	0.006276053277829874
1103	37	-0.0038580510633821063
1103	38	0.06329619869381276
1103	39	1.2028468145766524
1103	40	0.041531561800077554
1103	41	0.06817387487139115
1103	42	0.10445346605871464
1103	43	-9.014370986193398E-4
1103	44	-0.06590614071810563
1103	45	-0.040737912923724195
1103	46	0.09127218630119671
1103	47	0.07499322567762334
1103	48	0.10062253844025548
1103	49	-0.05818622539657525
1103	50	0.1189916563918132
1103	51	-0.04554015943438117
1103	52	-0.003483953487162239
1103	53	-8.139957118658003E-5
1103	54	0.04052390143557005
1103	55	0.07365764930552388
1103	56	0.025343225753019283
1103	57	-0.028618242987455744
1103	58	-0.14600726713394607
1103	59	0.10677866991054685
1103	60	0.08030148620746047
1103	61	0.18012658835794326
1104	1	-0.033930621798923255
1104	2	0.039440983087182246
1104	3	0.07030153718623211
1104	4	0.038269076122247725
1104	5	-0.008214562511362544
1104	6	0.018023716686371927
1104	7	0.11495618657876605
1104	8	0.07647627249356503
1104	9	0.011238128273426184
1104	10	0.08481523834436899
1104	11	0.13329805048499566
1104	12	0.11235991033423431
1104	13	0.1257098125908911
1104	14	0.06369423606674474
1104	15	-0.09163539720888991
1104	16	0.0785774233439227
1104	17	-0.009724405689233606
1104	18	0.07771031603424206
1104	19	0.06331783895617349
1104	20	0.04243519907882387
1104	21	-0.01840203980863464
1104	22	-0.09467032465799008
1104	23	0.07852003857964718
1104	24	-0.06933446473478
1104	25	-0.09518341488343651
1104	26	0.013912432425755128
1104	27	0.07371513609653846
1104	28	0.06479676465821171
1104	29	0.12352070701115991
1104	30	-0.07590445884270025
1104	31	0.018221391002590792
1104	32	-0.009484780595997222
1104	33	0.030909524805444732
1104	34	2.8483392386391415E-4
1104	35	-0.004979879970264278
1104	36	8.644490400442351E-4
1104	37	-0.043707351084393053
1104	38	-0.04083287183841122
1104	39	1.2205198506810788
1104	40	-0.10548164395201809
1104	41	-0.02775388115482258
1104	42	-0.023099648565825248
1104	43	0.16582829816999123
1104	44	0.09451068479854996
1104	45	-0.061370832565529554
1104	46	0.013560040985481692
1104	47	0.11039020844899738
1104	48	-0.0378216654098793
1104	49	0.048653361195512446
1104	50	0.045157198921046415
1104	51	-0.036671822280595734
1104	52	0.0869907156207006
1104	53	-0.009427258945606809
1104	54	0.0774981688567351
1104	55	0.0343188690919618
1104	56	-0.02113953200615981
1104	57	0.046342846376013824
1104	58	-0.0415812183203812
1104	59	-0.07693492329213569
1104	60	0.04363503913187827
1104	61	0.11450570163972174
1201	1	0.01981597817670888
1201	2	0.07566893097960303
1201	3	0.08920070468019503
1201	4	-0.03962088032324118
1201	5	-0.05172052643120395
1201	6	0.11749256619189907
1201	7	0.02926236040964625
1201	8	-0.06770951097164613
1201	9	0.0262245999158921
1201	10	0.1240148933231282
1201	11	-0.047932798689807044
1201	12	0.11560829442217369
1201	13	0.07778658196961175
1201	14	0.051270314769276126
1201	15	0.01614237113641792
1201	16	1.6233060152615053E-4
1201	17	-0.19749793659592996
1201	18	-0.09059347579178478
1201	19	-0.03473486430606698
1201	20	0.14411316020127884
1201	21	-0.0143229073165827
1201	22	-0.07403552909347866
1201	23	-0.09813523777870614
1201	24	-0.06404579700150492
1201	25	-0.025049979806944123
1201	26	0.03997459292400407
1201	27	-0.013303965736263024
1201	28	-0.0069814279092241804
1201	29	-0.08379525943990274
1201	30	0.023448650240375457
1201	31	-0.02386412227808421
1201	32	-0.029345263168991664
1201	33	0.015572005765967845
1201	34	-0.058461560995127115
1201	35	-0.0522823210529495
1201	36	0.17115256736502005
1201	37	-0.03611535329111604
1201	38	-0.020703125390042487
1201	39	1.246213746198805
1201	40	0.041865957417407174
1201	41	0.009026225075281502
1201	42	0.030172989597211597
1201	43	-0.015328898008711178
1201	44	0.05819975771895969
1201	45	-0.03025005038027473
1201	46	0.21311996433375668
1201	47	0.012442245486411707
1201	48	0.015514103892677156
1201	49	0.13706756555842503
1201	50	0.09881914577778161
1201	51	-0.04773938061083527
1201	52	0.031073743591022662
1201	53	0.08316220941726016
1201	54	-9.17880418072059E-4
1201	55	0.040953377935878166
1201	56	0.07401631283136823
1201	57	0.10778761924321145
1201	58	0.038585763543643736
1201	59	0.0551392467097358
1201	60	0.12225578634391354
1201	61	0.06376261345329226
1202	1	-0.013505785099930279
1202	2	-0.12148483507385066
1202	3	-0.08018492530938914
1202	4	-0.05905857556795269
1202	5	-0.07254662847910254
1202	6	0.06056788726097295
1202	7	-0.016555064095733485
1202	8	-0.04271818851416498
1202	9	0.02205937950630954
1202	10	-0.041205516256880514
1202	11	-0.0326603238546781
1202	12	-0.026009199503548075
1202	13	-0.22488610112653618
1202	14	0.005452890263896393
1202	15	0.04391050720028744
1202	16	-0.022052178249566623
1202	17	0.030200779127774524
1202	18	0.03713995010200222
1202	19	-0.2568799528170018
1202	20	-0.07664352150646181
1202	21	-0.015711314119776887
1202	22	0.042590642374761956
1202	23	-0.09535842417231777
1202	24	-0.0765414582302455
1202	25	-0.144452964881566
1202	26	0.002487609237782351
1202	27	0.01307576352441231
1202	28	-0.025030716350734394
1202	29	0.018946843996403118
1202	30	0.015118209421217443
1202	31	0.016399675014525883
1202	32	0.06784321305454455
1202	33	-0.010807723494707488
1202	34	-0.11677464672924742
1202	35	-0.08838089793597703
1202	36	-0.31895503416222937
1202	37	-0.3262923751585234
1202	38	-0.1664858397253468
1202	39	0.9838048603952672
1202	40	-0.2274849624021016
1202	41	-0.2950348648240606
1202	42	-0.025363282530520337
1202	43	-0.3249436151208229
1202	44	-0.1097974654674303
1202	45	-0.27322124093910816
1202	46	-0.2672687895711334
1202	47	-0.1819347069606536
1202	48	-0.14137586486816645
1202	49	-0.14339060868663012
1202	50	-0.14276363797786473
1202	51	-0.1032756527385672
1202	52	-0.11609737754746874
1202	53	-0.10843792942341679
1202	54	-0.16058466278531114
1202	55	-0.28115700040497416
1202	56	-0.12313745322208547
1202	57	-0.2906851332732785
1202	58	-0.20577383381838388
1202	59	-0.27391316564708035
1202	60	-0.1970777783905504
1202	61	-0.24029847644604985
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2403	13	0.045319222879548704
2403	14	0.17191218591444368
2403	15	0.08671615694796486
2403	16	-0.054626337862949015
2403	17	0.030585261011736975
2403	18	0.06653145412037276
2403	19	0.05790390962392422
2403	20	0.015973892318868366
2403	21	0.05037684971222944
2403	22	0.056581510930016066
2403	23	0.0400432792960288
2403	24	0.160256662080549
2403	25	0.00639209425921905
2403	26	0.08438225052152859
2403	27	0.14377824396656536
2403	28	0.02736562039131485
2403	29	0.07730264993985259
2403	30	0.10899586942174722
2403	31	0.03944722794753375
2403	32	-0.03257063897333268
2403	33	0.026807420819501715
2403	34	-0.011405250419208812
2403	35	-0.01782847222909112
2403	36	0.0690512670686445
2403	37	0.001734752174407106
2403	38	-0.023800340566396017
2403	39	1.2619347832318866
2403	40	-0.00805260718356493
2403	41	0.05935063166486998
2403	42	-0.03559477266482247
2403	43	0.1270789197895965
2403	44	0.07248966773951793
2403	45	0.19563305644695106
2403	46	0.0795979500758861
2403	47	-0.10330816169058465
2403	48	0.030145775587868684
2403	49	0.09415511528742115
2403	50	0.03320090424839606
2403	51	0.10729225904420048
2403	52	0.0512376823943228
2403	53	0.12212304032533439
2403	54	0.010381614949452
2403	55	-0.00808942237384258
2403	56	-0.09816749086926535
2403	57	0.043194662737725764
2403	58	0.09190058478626639
2403	59	0.04507863741275031
2403	60	-0.0512309837412559
2403	61	-0.03167433418774834
2404	1	-0.047196160605309956
2404	2	-0.14593863877072977
2404	3	-0.03471998013679212
2404	4	0.013472969132394041
2404	5	0.06322489060416103
2404	6	-0.012227650282234492
2404	7	-0.13244596660904762
2404	8	0.08630098029947675
2404	9	0.04356990744310707
2404	10	-0.04023509812154913
2404	11	0.0472234215193339
2404	12	-0.02212166045460151
2404	13	-0.08969047754121817
2404	14	-0.10856855182144898
2404	15	-0.009081167247650512
2404	16	-0.057987470670525454
2404	17	-0.031066582068213222
2404	18	-0.08739280700991259
2404	19	-0.010447522762881079
2404	20	-0.0023334472462508415
2404	21	-0.009433173216038426
2404	22	-0.09164091845848077
2404	23	-0.12889773710649877
2404	24	-0.05665986390902589
2404	25	0.014890670690981267
2404	26	-0.19328410539573326
2404	27	-0.05237686198218938
2404	28	0.05833137916578579
2404	29	0.021400510808106787
2404	30	-0.08113993646905016
2404	31	-0.024522637758330745
2404	32	0.021041193020423066
2404	33	-0.12344075877685867
2404	34	-0.05795215258353181
2404	35	0.1081837729773909
2404	36	-0.1617507183681255
2404	37	-0.047901167097940345
2404	38	0.0029828991221805268
2404	39	1.2249295676809808
2404	40	0.03824485558949675
2404	41	0.06814400010448907
2404	42	-0.0614936363298213
2404	43	0.04757326331047551
2404	44	0.03269022708845881
2404	45	0.06398952801337998
2404	46	0.02330275493365974
2404	47	0.035712725708947346
2404	48	0.05354520616057101
2404	49	0.15517454132486108
2404	50	0.04451386249878908
2404	51	-0.06376521279938885
2404	52	-0.03306527308452445
2404	53	-0.06747357334595705
2404	54	0.045539971169063165
2404	55	-0.10223389565359042
2404	56	0.006118331398866417
2404	57	-0.08950205423675328
2404	58	-0.02739191439486177
2404	59	0.017877145815582196
2404	60	-0.11446512922237417
2404	61	-0.0724664932242618
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	36.0
18	67.0
19	120.0
20	165.0
21	227.0
22	347.0
23	496.0
24	781.0
25	989.0
26	1312.0
27	1633.0
28	2263.0
29	2708.0
30	3522.0
31	4346.0
32	5752.0
33	6774.0
34	8747.0
35	11284.0
36	16865.0
37	25058.0
38	62797.0
39	388963.0
40	362164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.611513962663373	11.067311718939411	32.565405214784775	30.75576910361244
2	28.03950856809	16.723897502604324	28.584956098528902	26.651637830776775
3	27.51168700642923	19.871988433114616	21.047521649339114	31.56880291111704
4	26.455555310660994	20.02193030790593	20.445548918912962	33.076965462520114
5	27.26862974146481	20.240241563994623	19.75006061140376	32.7410680831368
6	29.230235172246587	22.90515968349827	22.487051200105796	25.377553944149344
7	26.90760618015913	22.223116087368584	22.24658923100659	28.622688501465692
8	25.22150713010513	24.17171761698001	22.57697648277534	28.029798770139518
9	25.572502259152323	24.43157523528245	22.926759383747328	27.0691631218179
10	25.814507063983598	22.352163276101475	22.868131625928456	28.965198033986468
11	25.16563443609354	23.022525401688306	22.687509642723324	29.12433051949483
12	26.91234488990765	23.07762667783386	22.292764100416566	27.717264331841925
13	25.391329263185735	21.519693196094423	22.47768398316105	30.611293557558795
14	25.874016442220803	22.60055982896564	22.345110312754844	29.180313416058716
15	26.38756033589738	22.314804610874788	22.09285667056049	29.20477838266734
16	25.799299111767425	22.663705891428446	22.115337991227875	29.42165700557625
17	26.100041876969872	21.73987789557206	21.986070397390403	30.174009830067668
18	27.576094862357014	22.57488263428181	21.87432500936722	27.97469749399396
19	25.779242247250444	21.701968217583918	22.848184963963767	29.67060457120187
20	26.034140750699784	22.13451323532653	22.01163738952194	29.819708624451742
21	26.52454210839523	22.677371007912544	22.545678957924668	28.25240792576756
22	26.70362125586829	21.960723810363447	22.061999955918978	29.27365497784929
23	26.196689515329176	23.112560886910142	21.831346013973686	28.859403583787003
24	26.66196469110225	23.84739150558727	21.765995900465054	27.72464790284543
25	25.862555376782524	22.22994864561063	22.169667849507395	29.737828128099448
26	26.181261158008418	22.53520971545701	22.857111370699347	28.426417755835224
27	26.629124330519495	23.45462960922175	21.77635494038042	28.139891119878335
28	26.061581186220273	22.805316171122524	22.002270172577195	29.13083247008001
29	26.350201670670693	24.070992484185933	21.75166956866721	27.82713627647616
30	27.825813845848668	24.1470322452668	21.40320909832272	26.623944810561813
31	26.344250732846973	23.481078221771618	22.623922770051355	27.550748275330054
32	26.768861166824625	23.912521213991315	21.415772189283906	27.902845429900157
33	26.986401005047277	25.44334486786714	21.236362434153975	26.33389169293161
34	26.725220956117347	24.299552577637698	21.22005245641489	27.755174009830068
35	27.091203632276123	24.68052280090807	21.660311652817878	26.567961913997927
36	27.51945075047938	25.375239690551233	20.63884419563157	26.466465363337814
37	27.907363734544095	25.094994600074937	20.47376077229949	26.523880893081486
38	28.117740406867824	25.476185228449893	20.30680390557845	26.099270459103835
39	27.93023155338371	26.772919419058645	19.883393326951197	25.413455700606452
40	27.15115382072249	25.9434440501642	20.84007405611514	26.065328072998174
41	27.5539441493465	26.053867007559894	20.5963060104472	25.795882832646406
42	27.874192766304468	26.766436710674217	19.401379735954684	25.957990787066628
43	27.342685856604437	27.3989993608252	19.564810121002402	25.69350466156796
44	27.475149324458354	26.796191399792818	20.06744396200216	25.661215313746666
45	27.61246170461308	27.39040356174649	18.976659099424744	26.02047563421569
46	27.230169050715215	27.17374534394217	19.78444380771859	25.811641797624034
47	28.277534107689934	27.23083026602896	18.807828789314758	25.683806836966344
48	28.393026382491023	27.877939653082368	18.5965704965727	25.132463467853917
49	28.009962310727115	27.503691785501754	18.494963743360294	25.991382160410836
50	28.06484318176809	27.736880386149743	18.376936809856517	25.821339622225654
51	27.94582442529369	27.96940777148399	18.52504904013577	25.559718763086554
52	27.479006413788543	27.83275660664301	18.340790372705033	26.347446606863418
53	27.681668907451893	28.024619250181836	18.16435608648696	26.129355755879306
54	27.55185030085297	28.178351810627934	18.08490004628507	26.18489784223403
55	26.90914901589121	28.1712988472813	18.229044984681845	26.690507152145642
56	26.90848780057746	28.05393312909127	18.18584558418373	26.851733486147538
57	26.74902470741222	28.220449185603137	18.090189768795046	26.940336338189592
58	26.396376540080666	28.09250402239316	18.292521654801526	27.218597782724647
59	26.382601221044276	28.00786846223359	18.229816402547883	27.379713914174253
60	26.15337991227877	28.06341054858831	18.477000727336844	27.30620881179608
61	26.14963302550087	27.744263957153247	18.56009345176434	27.546009565581535
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	2.5
3	5.0
4	4.0
5	3.0
6	2.5
7	4.5
8	7.0
9	8.0
10	9.0
11	9.5
12	12.5
13	15.0
14	17.0
15	19.0
16	29.0
17	54.5
18	70.0
19	72.5
20	105.0
21	135.0
22	210.5
23	286.0
24	356.0
25	622.0
26	818.0
27	965.5
28	1113.0
29	1502.0
30	2312.0
31	2733.0
32	3272.0
33	3811.0
34	4591.0
35	6480.5
36	7590.0
37	8690.5
38	9791.0
39	11702.5
40	15693.0
41	17772.0
42	20095.0
43	25363.5
44	28309.0
45	31817.5
46	35326.0
47	39403.5
48	47447.5
49	51414.0
50	55937.5
51	60461.0
52	63958.0
53	70542.0
54	73629.0
55	74395.5
56	75162.0
57	74867.0
58	71836.0
59	69100.0
60	64892.0
61	57839.5
62	54995.0
63	46360.5
64	37726.0
65	33087.0
66	24204.5
67	19961.0
68	16962.5
69	13964.0
70	11770.5
71	7852.5
72	6128.0
73	4960.0
74	3792.0
75	2977.0
76	1764.0
77	1366.0
78	1076.5
79	787.0
80	588.5
81	302.0
82	214.0
83	168.5
84	92.0
85	61.0
86	47.5
87	34.0
88	30.5
89	19.0
90	11.0
91	10.0
92	9.0
93	6.5
94	4.5
95	5.0
96	5.5
97	6.0
98	7.5
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.030305701880055543
3	2.2040510458222213E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.0726675629807585
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	907420.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.02138798350926
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.81256281287986	61.47274366369023
2	15.283079004058395	23.54247915108412
3	3.3576732217218894	7.758379557962434
4	0.8625959615941459	2.657533529238038
5	0.2779236981765197	1.0703034493532726
6	0.13407556025673434	0.6196011447384184
7	0.07488029098820231	0.40371687611642815
8	0.05186414757197142	0.31957189060598323
9	0.03962239310266467	0.27465945407959114
>10	0.10463414923894784	1.0512978744465633
>50	6.22148806111281E-4	0.029589837127516474
>100	3.110744030556405E-4	0.04863754604765866
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.5553720152782025E-4	0.7514860255097605
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGATTCATCTGGGGGGGGGGGGGGG	6273	0.6913006105221396	TruSeq Adapter, Index 10 (97% over 36bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.848339247536973	0.0	0.0	0.0	0.0
2	0.8504330960305041	0.0	0.0	0.0	0.0
3	0.8520861343148707	0.0	0.0	0.0	0.0
4	0.853298362390073	0.0	0.0	0.0	0.0
5	0.8544003879129841	0.0	0.0	0.0	0.0
6	0.857265654272553	0.0	0.0	0.0	0.0
7	0.8607921359458685	0.0	0.0	0.0	0.0
8	0.8948447246038218	2.2040510458222213E-4	0.0	0.0	0.0
9	0.9002446496660863	2.2040510458222213E-4	0.0	0.0	0.0
10	0.903991536443984	2.2040510458222213E-4	0.0	0.0	1.1020255229111106E-4
11	0.9102730819245773	2.2040510458222213E-4	0.0	0.0	1.1020255229111106E-4
12	0.9305503515461419	2.2040510458222213E-4	0.0	0.0	1.1020255229111106E-4
13	0.9601948381124507	2.2040510458222213E-4	0.0	0.0	1.1020255229111106E-4
14	0.9930351986952017	2.2040510458222213E-4	0.0	0.0	1.1020255229111106E-4
15	1.0398712834189239	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
16	1.0965153952965552	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
17	1.1841264243679883	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
18	1.3151572590421194	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
19	1.4880650635868726	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
20	1.7157435366203082	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
21	2.038416609728681	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
22	2.41321549007075	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
23	2.87044587952657	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
24	3.4417359106036898	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
25	4.080690308787552	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
26	4.815410724912389	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
27	5.676092658305967	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
28	6.565427255295233	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
29	7.550638072777765	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
30	8.694430363007207	2.2040510458222213E-4	0.0	0.0	3.306076568733332E-4
31	9.885168940512662	2.2040510458222213E-4	0.0	0.0	6.612153137466664E-4
32	11.175861232946154	2.2040510458222213E-4	0.0	0.0	6.612153137466664E-4
33	12.597033347292323	2.2040510458222213E-4	0.0	0.0	6.612153137466664E-4
34	14.03098895770426	2.2040510458222213E-4	0.0	0.0	6.612153137466664E-4
35	15.548037292543695	2.2040510458222213E-4	0.0	0.0	6.612153137466664E-4
36	17.16404751934055	2.2040510458222213E-4	1.1020255229111106E-4	0.0	6.612153137466664E-4
37	18.808049194419343	2.2040510458222213E-4	1.1020255229111106E-4	0.0	6.612153137466664E-4
38	20.536245619448547	2.2040510458222213E-4	1.1020255229111106E-4	0.0	6.612153137466664E-4
39	22.3208657512508	2.2040510458222213E-4	1.1020255229111106E-4	0.0	6.612153137466664E-4
40	24.21932511956977	2.2040510458222213E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
41	26.17542042273699	2.2040510458222213E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
42	28.18981287606621	2.2040510458222213E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
43	30.20167067069273	2.2040510458222213E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
44	32.248242269290955	2.2040510458222213E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
45	34.29305062705252	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
46	36.301051332348855	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
47	38.32040290053118	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
48	40.41865949615393	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
49	42.389081131119	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
50	44.372616869806706	3.306076568733332E-4	1.1020255229111106E-4	0.0	9.918229706199996E-4
>>END_MODULE