##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-22B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 534108 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.564013270724274 40.0 40.0 40.0 40.0 40.0 2 39.3374448613389 40.0 40.0 40.0 40.0 40.0 3 39.299070599953566 40.0 40.0 40.0 40.0 40.0 4 39.18817168063388 40.0 40.0 40.0 40.0 40.0 5 39.227497060519596 40.0 40.0 40.0 40.0 40.0 6 39.365132145558576 40.0 40.0 40.0 40.0 40.0 7 39.37038950923783 40.0 40.0 40.0 40.0 40.0 8 39.40585050214563 40.0 40.0 40.0 40.0 40.0 9 39.39605473050394 40.0 40.0 40.0 40.0 40.0 10-11 39.40322556486703 40.0 40.0 40.0 40.0 40.0 12-13 39.315531690219956 40.0 40.0 40.0 40.0 40.0 14-15 39.35371497899301 40.0 40.0 40.0 40.0 40.0 16-17 39.45582166902574 40.0 40.0 40.0 40.0 40.0 18-19 39.487598013884835 40.0 40.0 40.0 40.0 40.0 20-21 39.46260306904222 40.0 40.0 40.0 40.0 40.0 22-23 39.50259123622938 40.0 40.0 40.0 40.0 40.0 24-25 39.477128221258624 40.0 40.0 40.0 40.0 40.0 26-27 39.45126828281921 40.0 40.0 40.0 40.0 40.0 28-29 39.458611366989444 40.0 40.0 40.0 40.0 40.0 30-31 39.43079757629059 40.0 40.0 40.0 40.0 40.0 32-33 39.42910004899303 40.0 40.0 40.0 40.0 40.0 34-35 39.432226690756465 40.0 40.0 40.0 40.0 40.0 36-37 39.40802656422463 40.0 40.0 40.0 40.0 40.0 38-39 39.35485351539916 40.0 40.0 40.0 40.0 40.0 40-41 39.396770351834434 40.0 40.0 40.0 40.0 40.0 42-43 39.367529998221016 40.0 40.0 40.0 40.0 40.0 44-45 39.26095320425702 40.0 40.0 40.0 40.0 40.0 46-47 39.25533119122248 40.0 40.0 40.0 40.0 40.0 48-49 39.33205042561795 40.0 40.0 40.0 40.0 40.0 50-51 39.290091278508775 40.0 40.0 40.0 40.0 40.0 52-53 39.23254607877146 40.0 40.0 40.0 40.0 40.0 54-55 39.140157987716535 40.0 40.0 40.0 40.0 40.0 56-57 39.161036762844546 40.0 40.0 40.0 40.0 40.0 58-59 39.11174006727387 40.0 40.0 40.0 40.0 40.0 60-61 39.023416693096415 40.0 40.0 40.0 40.0 40.0 62-63 39.37464038446666 40.0 40.0 40.0 40.0 40.0 64-65 39.37855163985867 40.0 40.0 40.0 40.0 40.0 66-67 39.367681968095155 40.0 40.0 40.0 40.0 40.0 68-69 39.35987861782857 40.0 40.0 40.0 40.0 40.0 70-71 39.3713594780116 40.0 40.0 40.0 40.0 40.0 72-73 39.366345472362525 40.0 40.0 40.0 40.0 40.0 74-75 39.36215278463578 40.0 40.0 40.0 40.0 40.0 76-77 39.352973800912395 40.0 40.0 40.0 40.0 40.0 78-79 39.374219103811036 40.0 40.0 40.0 40.0 40.0 80-81 39.37138588959828 40.0 40.0 40.0 40.0 40.0 82-83 39.35168293953545 40.0 40.0 40.0 40.0 40.0 84-85 39.305536866598906 40.0 40.0 40.0 40.0 40.0 86-87 39.327846699614824 40.0 40.0 40.0 40.0 40.0 88-89 39.34543209285344 40.0 40.0 40.0 40.0 40.0 90-91 39.44077842370423 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08897164380088896 1101 2 -0.007049819753625286 1101 3 -0.012541620022190614 1101 4 0.04367438997388717 1101 5 0.07174675074065107 1101 6 0.05078636531523273 1101 7 -0.025944761316203824 1101 8 -0.21955938147360854 1101 9 -0.02829120420507536 1101 10-11 0.01147046202527946 1101 12-13 0.05638744121571193 1101 14-15 -7.006748516218408E-4 1101 16-17 -0.09339050878917021 1101 18-19 0.00993301102200661 1101 20-21 0.12580233753163128 1101 22-23 -0.025559602594505293 1101 24-25 -0.11379678835901075 1101 26-27 -0.02496992934056408 1101 28-29 0.012328209805488655 1101 30-31 -0.04007337339718475 1101 32-33 -0.0497423888572186 1101 34-35 0.04912089154829857 1101 36-37 -0.0065971398459439 1101 38-39 -0.02569883865105993 1101 40-41 -0.08741403943879789 1101 42-43 0.022383682032355523 1101 44-45 0.019320898424616928 1101 46-47 0.14039540715483412 1101 48-49 -0.0266508813237607 1101 50-51 0.09898787805395415 1101 52-53 -0.09138488803682066 1101 54-55 -0.08798510315712349 1101 56-57 -0.021499507868099954 1101 58-59 0.01535050835095575 1101 60-61 0.1786688776476666 1101 62-63 0.1589048625045777 1101 64-65 0.06204414577928219 1101 66-67 0.08263989633075397 1101 68-69 -0.06626136887703638 1101 70-71 -0.20593408479827247 1101 72-73 -0.2268319646480066 1101 74-75 0.11470801931553609 1101 76-77 -0.17358347654072048 1101 78-79 0.13122622034690323 1101 80-81 0.16812262159014324 1101 82-83 0.06088113115698235 1101 84-85 0.22698716173538713 1101 86-87 0.2690336786490093 1101 88-89 0.19386421116765717 1101 90-91 0.3536595394736892 1102 1 0.0776708270734261 1102 2 -0.11281567707104045 1102 3 0.057257191964403376 1102 4 -0.08645663024169892 1102 5 -0.197830669745926 1102 6 -0.007894480085752775 1102 7 -0.007598301765519011 1102 8 -0.19973092964138317 1102 9 -0.34628440518742565 1102 10-11 0.05322332620219328 1102 12-13 -0.14741069328869116 1102 14-15 -0.18013969540594843 1102 16-17 -0.021643935518262936 1102 18-19 -0.13001242746623376 1102 20-21 -0.11404147862874225 1102 22-23 -0.04525195309474128 1102 24-25 -0.06320641102562519 1102 26-27 -0.11630299652018294 1102 28-29 -0.01316508191108312 1102 30-31 -0.09141986924155532 1102 32-33 -0.09604835683786916 1102 34-35 -0.09204007165081407 1102 36-37 -0.042111127108007906 1102 38-39 -0.0414519214258533 1102 40-41 -0.02824202796959696 1102 42-43 -0.04551773767244782 1102 44-45 -0.1909445742738356 1102 46-47 0.0029206351022708077 1102 48-49 0.16205693826950096 1102 50-51 0.021199288314242892 1102 52-53 -0.07882805328191722 1102 54-55 -0.09410145411796123 1102 56-57 -0.23100751857136714 1102 58-59 -0.20570699210556143 1102 60-61 -0.252607887928157 1102 62-63 0.06393471007659457 1102 64-65 0.03690629247196142 1102 66-67 0.009671841194681008 1102 68-69 0.11116575427311659 1102 70-71 0.01903847612505416 1102 72-73 -0.23237943603700728 1102 74-75 0.15323942573292015 1102 76-77 0.1930200504698334 1102 78-79 0.034228775537087586 1102 80-81 0.11550990872152767 1102 82-83 0.10833751035512051 1102 84-85 0.2024700058681148 1102 86-87 0.30115095334499387 1102 88-89 -0.7870682689159239 1102 90-91 0.3536595394736892 1103 1 0.05656837911995183 1103 2 -0.0013718131740105832 1103 3 -0.09129407835314396 1103 4 -0.0844852148001678 1103 5 0.2046817746933698 1103 6 0.03767591740257359 1103 7 0.0241200542168869 1103 8 0.03875820536871544 1103 9 -0.05277327759958439 1103 10-11 -0.011971440375269538 1103 12-13 -0.030459030618928296 1103 14-15 -0.12716731839253015 1103 16-17 -0.07859100272402486 1103 18-19 -0.039011620363730515 1103 20-21 -0.11044137667894915 1103 22-23 -0.07645187122597719 1103 24-25 -0.13031852876218863 1103 26-27 -0.08249158046076843 1103 28-29 -0.03233376633400553 1103 30-31 0.01215667612802207 1103 32-33 -0.1546927789864796 1103 34-35 -0.004566412988744162 1103 36-37 -0.21606146617661892 1103 38-39 0.004452506248007637 1103 40-41 -0.02563293653598464 1103 42-43 0.05262906476549034 1103 44-45 0.08596291209895668 1103 46-47 -0.0377890090481543 1103 48-49 -0.10379571838174684 1103 50-51 0.07048905358347213 1103 52-53 0.1689608619477454 1103 54-55 0.1339658019086727 1103 56-57 0.20483248884713845 1103 58-59 -0.1790918235408654 1103 60-61 -0.08776044314758735 1103 62-63 -0.09407338137164345 1103 64-65 0.010927137501909101 1103 66-67 -0.21979061114875975 1103 68-69 -0.2006413856352438 1103 70-71 -0.08187210902060826 1103 72-73 -0.05145124906105991 1103 74-75 -0.3952450785394106 1103 76-77 -0.3519254265522065 1103 78-79 -0.18944927710468562 1103 80-81 -0.03165667280723028 1103 82-83 -0.33187295958109075 1103 84-85 -0.35300097630360483 1103 86-87 -0.13099615206170512 1103 88-89 -0.08984133060240396 1103 90-91 0.3536595394736892 1104 1 -0.066513458557651 1104 2 0.00452953814242818 1104 3 0.07199887756053869 1104 4 -0.21320250944258845 1104 5 0.04219495783867444 1104 6 0.015094628190794879 1104 7 -0.053489151552923886 1104 8 0.03850076702121186 1104 9 -0.06471879274639747 1104 10-11 0.038164208024085156 1104 12-13 0.027465537126587947 1104 14-15 0.012833104407675933 1104 16-17 0.060929306953639184 1104 18-19 -0.0366552138836127 1104 20-21 -0.07696593549867714 1104 22-23 -0.03846937985227328 1104 24-25 0.01313758105312246 1104 26-27 0.04734660450492356 1104 28-29 0.0098851831014386 1104 30-31 -0.03082519278981266 1104 32-33 -0.018927993291654843 1104 34-35 -0.0663867060092116 1104 36-37 -0.15452077608400572 1104 38-39 -0.009608202637160446 1104 40-41 -0.11734219397035872 1104 42-43 -0.05505130399981795 1104 44-45 -0.014875705590917221 1104 46-47 -0.0693632869823233 1104 48-49 -0.05583693392915734 1104 50-51 0.059249193411950785 1104 52-53 -0.0010432285699550903 1104 54-55 -0.10283109303591687 1104 56-57 0.014269372427008875 1104 58-59 0.05926442613410643 1104 60-61 0.005486413557747483 1104 62-63 -0.0031409277313656503 1104 64-65 0.08181153174817268 1104 66-67 0.09007370529317171 1104 68-69 -0.28306306736558184 1104 70-71 -0.12469942144933555 1104 72-73 0.21475517376111952 1104 74-75 -0.04063386598090091 1104 76-77 -0.09254086228278169 1104 78-79 0.06759556466148808 1104 80-81 -0.7120391619750137 1104 82-83 -0.5229026526267972 1104 84-85 -0.04956406481583997 1104 86-87 -0.43941729067152124 1104 88-89 -0.01696199965855527 1104 90-91 0.3536595394736892 1201 1 0.029906099127252617 1201 2 0.12621894796119193 1201 3 0.03760039472128085 1201 4 0.029292250364065353 1201 5 -0.07567018026002614 1201 6 -0.0801100601781144 1201 7 0.022420218055380303 1201 8 -0.00639806516426944 1201 9 0.06933319567527008 1201 10-11 0.0386946439226179 1201 12-13 -0.0015202181029110307 1201 14-15 0.011569754391281606 1201 16-17 0.09110426524598125 1201 18-19 -0.03604777922561908 1201 20-21 0.02562493016700529 1201 22-23 0.06320035250573852 1201 24-25 0.07631934090254333 1201 26-27 0.19080955241668818 1201 28-29 0.053617626671325525 1201 30-31 0.028999991508143808 1201 32-33 0.08825589677530132 1201 34-35 0.053038660857978925 1201 36-37 0.10203826518621639 1201 38-39 0.16678605491865284 1201 40-41 0.05813433917700195 1201 42-43 0.069410151375223 1201 44-45 -0.012871564431883087 1201 46-47 0.17041515194809875 1201 48-49 0.10718549821221757 1201 50-51 0.06685055927496109 1201 52-53 0.13648225234184252 1201 54-55 0.03501053356653472 1201 56-57 0.14547004561080712 1201 58-59 0.06836461168566643 1201 60-61 0.25252054181888184 1201 62-63 0.2589690695341602 1201 64-65 -0.15070954131270753 1201 66-67 0.15957953426977411 1201 68-69 0.2103721585180125 1201 70-71 0.2470058814426892 1201 72-73 0.2078006081544359 1201 74-75 0.16200668187703826 1201 76-77 -0.02637154948933329 1201 78-79 0.21865890556883016 1201 80-81 0.20863526164058754 1201 82-83 -0.09137388727450713 1201 84-85 -0.05650210885065832 1201 86-87 -0.222114593368822 1201 88-89 -0.3023650497674808 1201 90-91 -2.313007127192982 1202 1 0.028190608776647252 1202 2 -0.17968348752667396 1202 3 -0.03356326701264578 1202 4 0.0444887455664329 1202 5 -0.07080240577451491 1202 6 -0.030641419661847635 1202 7 0.02935561976917711 1202 8 0.04916047194238615 1202 9 0.0861208976243546 1202 10-11 0.030090828980902984 1202 12-13 -0.06565997313239791 1202 14-15 0.03806381749533472 1202 16-17 0.0015645194436118004 1202 18-19 -0.06799149005156835 1202 20-21 0.01615489616791166 1202 22-23 -0.0032377076531702187 1202 24-25 -0.09086752796977748 1202 26-27 0.015407725636023883 1202 28-29 0.04624252463166556 1202 30-31 0.07029416044156989 1202 32-33 0.06008481636018814 1202 34-35 0.05960557550194778 1202 36-37 -0.14222361177592546 1202 38-39 -0.13678548712005778 1202 40-41 -0.06888125825990699 1202 42-43 -0.11741459832433776 1202 44-45 -0.1275020170409391 1202 46-47 -0.10157533165801169 1202 48-49 -0.019514079748532254 1202 50-51 -0.04417560026750067 1202 52-53 -0.0025219946800874027 1202 54-55 0.025688821778615534 1202 56-57 0.037073048223831506 1202 58-59 -0.15474059869310253 1202 60-61 -0.114557052264189 1202 62-63 -0.030929437583345987 1202 64-65 -0.5173821501333222 1202 66-67 -0.11748827352786151 1202 68-69 -0.2500016396436635 1202 70-71 -0.16360868713542231 1202 72-73 -0.11523971299033775 1202 74-75 -0.3785895729712436 1202 76-77 -0.21563246850876538 1202 78-79 -0.1972993177486586 1202 80-81 0.3918368070172349 1202 82-83 0.0851695562044199 1202 84-85 -1.1313433965951702 1202 86-87 -0.01471918176289222 1202 88-89 -0.2907140125410024 1202 90-91 0.3536595394736892 1203 1 0.06980037901167435 1203 2 0.10502288644632785 1203 3 0.03125171006028182 1203 4 -0.07171702068752239 1203 5 -0.03510761777971538 1203 6 -0.04446101809548253 1203 7 -0.06829640547419302 1203 8 0.0971309599374095 1203 9 0.0996852765265075 1203 10-11 0.05855140138259429 1203 12-13 0.019163676012638575 1203 14-15 0.016031362377795233 1203 16-17 0.06166422580634645 1203 18-19 -9.163822684925549E-4 1203 20-21 -0.028325195135984416 1203 22-23 0.08511947387845709 1203 24-25 -0.007372098780841441 1203 26-27 -0.011827972746615956 1203 28-29 -0.02856196058200311 1203 30-31 0.08575471262340528 1203 32-33 0.013054222501210688 1203 34-35 -0.09944222077023568 1203 36-37 0.00763755201308669 1203 38-39 -0.03183210702346173 1203 40-41 0.03338528732445667 1203 42-43 -0.09845979041809727 1203 44-45 0.026509969210074757 1203 46-47 -0.11561712342657415 1203 48-49 -0.13964822191935156 1203 50-51 -0.12085810549509546 1203 52-53 -0.09990709035609768 1203 54-55 -0.19636090025072406 1203 56-57 -0.0157383694980453 1203 58-59 0.13822442128101642 1203 60-61 -0.1016861448500066 1203 62-63 -0.020312766810491212 1203 64-65 -0.13059189844841512 1203 66-67 0.2006680508250298 1203 68-69 0.1539981097945997 1203 70-71 -0.29603205878292727 1203 72-73 -0.0386475673668798 1203 74-75 -0.14563067954434672 1203 76-77 0.20209646464370223 1203 78-79 -0.19275431888787153 1203 80-81 -0.13985525392904208 1203 82-83 0.09191782101720491 1203 84-85 0.39389998747174104 1203 86-87 0.15532130406707267 1203 88-89 0.08050388728101865 1203 90-91 -0.44634046052630794 1204 1 -0.0076265352773887685 1204 2 -0.015015389570621096 1204 3 -0.24483554363550297 1204 4 -0.2654144969440253 1204 5 -0.18977397198909784 1204 6 -0.07593067393680997 1204 7 -0.23644529136870318 1204 8 -0.2796404450137402 1204 9 -0.21384477470346752 1204 10-11 -0.13910386710283973 1204 12-13 -0.27143982220700735 1204 14-15 -0.18129582596233007 1204 16-17 -0.08895580661109648 1204 18-19 -0.20525563276159176 1204 20-21 -0.1759404300322558 1204 22-23 -0.18142741749706914 1204 24-25 -0.10256220950789441 1204 26-27 -0.013543883982706006 1204 28-29 -0.2590643007568474 1204 30-31 -0.2160451715070124 1204 32-33 -0.14692681053809054 1204 34-35 -0.19109715750973777 1204 36-37 -0.1968812427553388 1204 38-39 -0.04539280190161321 1204 40-41 -0.2838563822450979 1204 42-43 -0.2631503890403408 1204 44-45 -0.24693946809204448 1204 46-47 -0.24514948969441974 1204 48-49 -0.29436952616042333 1204 50-51 -0.1463151342765059 1204 52-53 -0.21257113175158082 1204 54-55 -0.2635047279785283 1204 56-57 -0.15811503510917646 1204 58-59 -0.22269210300635223 1204 60-61 -0.09584235024651377 1204 62-63 -0.25903674827073075 1204 64-65 -0.35527696792738084 1204 66-67 -0.1857844218339011 1204 68-69 -0.023395321049221707 1204 70-71 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0.04739212039311269 2401 44-45 0.13763216492997543 2401 46-47 0.05378041770097752 2401 48-49 0.050857056029038006 2401 50-51 -0.06531425203490926 2401 52-53 -0.04788491474314327 2401 54-55 0.08654860974053236 2401 56-57 -0.10936866863805506 2401 58-59 0.03503022070241002 2401 60-61 0.20517862243080032 2401 62-63 0.03716177664594511 2401 64-65 0.2210258321232459 2401 66-67 -0.06552104363003508 2401 68-69 -0.04605802386437574 2401 70-71 0.08396904454598086 2401 72-73 0.19920599889852042 2401 74-75 -0.12379077998888022 2401 76-77 -0.12919587538613087 2401 78-79 0.11493407115345633 2401 80-81 -0.164122495308348 2401 82-83 -0.012142580915536882 2401 84-85 -0.02104290315462265 2401 86-87 -0.4783783296325623 2401 88-89 -0.014572513398100284 2401 90-91 0.3536595394736892 2402 1 0.05996378559623139 2402 2 0.10621422509238698 2402 3 0.037035337203484175 2402 4 0.2304442930048225 2402 5 0.19599598038497845 2402 6 0.18123325864926443 2402 7 0.1697821584797623 2402 8 0.17907573978124702 2402 9 0.11371972875221559 2402 10-11 0.1379001899199821 2402 12-13 0.10117687733277592 2402 14-15 0.1328167604297974 2402 16-17 0.07867299985448284 2402 18-19 0.13505922748760213 2402 20-21 0.05731031963500044 2402 22-23 0.07442559961298656 2402 24-25 0.04282914085531786 2402 26-27 -0.05555130849482737 2402 28-29 0.07568017094821755 2402 30-31 0.09574424816232607 2402 32-33 0.09703938517588995 2402 34-35 -0.039171514923012296 2402 36-37 -0.03981685404895785 2402 38-39 -0.009356658117162908 2402 40-41 0.030755215317185502 2402 42-43 0.029865472710149277 2402 44-45 -0.0021008878088579763 2402 46-47 0.11701623606786882 2402 48-49 0.020401496600925384 2402 50-51 0.001919640452641147 2402 52-53 0.08513544415473717 2402 54-55 0.1732358415936801 2402 56-57 0.07997094817615391 2402 58-59 0.21715176262662794 2402 60-61 0.1895953685709202 2402 62-63 -0.09491687678820426 2402 64-65 0.20005479970714646 2402 66-67 0.029773692435412613 2402 68-69 0.06546589757464716 2402 70-71 -0.04510430837242296 2402 72-73 0.03431481867030328 2402 74-75 0.0872666023451032 2402 76-77 0.2882741145146852 2402 78-79 0.23240598312236926 2402 80-81 0.004810909073526659 2402 82-83 0.12706090801869863 2402 84-85 -0.03693780218958409 2402 86-87 -0.09763298801765785 2402 88-89 -0.20812090049487608 2402 90-91 -0.0673930921052559 2403 1 0.05634179558426666 2403 2 0.08365743498405465 2403 3 0.08699695309287137 2403 4 0.17102548560730213 2403 5 0.16832267000044254 2403 6 0.1544419661255887 2403 7 0.08680816575640904 2403 8 0.11948236634876963 2403 9 0.0813943866640372 2403 10-11 0.07716652256322476 2403 12-13 0.06070307857167734 2403 14-15 0.15498205302697698 2403 16-17 0.06398291071351281 2403 18-19 0.12257265171777476 2403 20-21 0.11022832872469479 2403 22-23 0.029294664878221965 2403 24-25 0.06451310078884376 2403 26-27 0.10517245657808161 2403 28-29 0.10413749720908072 2403 30-31 0.03503406044769264 2403 32-33 0.18238116805293458 2403 34-35 0.023125523427061978 2403 36-37 0.06928799637687177 2403 38-39 0.12361075392772847 2403 40-41 0.10165223462470863 2403 42-43 0.12449160970721351 2403 44-45 0.16423061566874964 2403 46-47 0.14553575218673842 2403 48-49 0.1054299049501708 2403 50-51 0.14242616896402183 2403 52-53 -0.04090947641464737 2403 54-55 0.07327561461106313 2403 56-57 0.06070623989364776 2403 58-59 0.11249122526236022 2403 60-61 -0.044574069348691125 2403 62-63 0.14181662072523693 2403 64-65 0.06880155247310427 2403 66-67 -0.17544282544579204 2403 68-69 0.13001250533025654 2403 70-71 0.26632130088309225 2403 72-73 0.10696728934367883 2403 74-75 0.23738192455555662 2403 76-77 0.28998439063799 2403 78-79 -0.15629818243173332 2403 80-81 -0.20872512556256595 2403 82-83 -0.45062289268149414 2403 84-85 -0.24053460828437068 2403 86-87 -0.18869578995002456 2403 88-89 -0.938443481140034 2403 90-91 0.3536595394736892 2404 1 -0.008020240623679342 2404 2 -0.06636305841038848 2404 3 -0.0025292022775289524 2404 4 0.04710777764358198 2404 5 0.017245562743816834 2404 6 0.13584163056468412 2404 7 0.03851157473674505 2404 8 0.15309448192561348 2404 9 0.03469008816227159 2404 10-11 0.1225429277367951 2404 12-13 0.09546911477569608 2404 14-15 0.041860481493237955 2404 16-17 -0.03603910617068351 2404 18-19 0.06666352640730366 2404 20-21 0.028707866291490802 2404 22-23 0.039944349712421 2404 24-25 0.10687938507595618 2404 26-27 0.038511425185348 2404 28-29 0.07921222192430832 2404 30-31 0.09318271569768655 2404 32-33 -0.008799124385873824 2404 34-35 0.052716775114035386 2404 36-37 0.11527503071163636 2404 38-39 0.18247400178861284 2404 40-41 0.07368588422596645 2404 42-43 0.14013922161876025 2404 44-45 0.10967519476603371 2404 46-47 -0.01294904418878673 2404 48-49 0.14373851069659338 2404 50-51 0.05212212560292073 2404 52-53 0.10559628673096455 2404 54-55 0.04386727644217103 2404 56-57 0.12858357817972177 2404 58-59 0.018310521218026565 2404 60-61 -0.1334033973216222 2404 62-63 0.06927941019941386 2404 64-65 -0.1412824034229203 2404 66-67 -0.03617419318978676 2404 68-69 0.02257663681131561 2404 70-71 0.05334923376788936 2404 72-73 -0.03597143442440398 2404 74-75 -0.04948897466301361 2404 76-77 0.14584619399447973 2404 78-79 0.0421694160565238 2404 80-81 0.06879660988185776 2404 82-83 0.048640836563748735 2404 84-85 -0.2165339535465307 2404 86-87 0.026383575129344194 2404 88-89 0.5015565188599638 2404 90-91 0.3536595394736892 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 361.0 32 2332.0 33 4157.0 34 5460.0 35 6869.0 36 9938.0 37 14855.0 38 38329.0 39 220765.0 40 231031.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.368652032922178 10.286870820133757 33.42732181506362 30.91715533188044 2 27.083653223178878 16.849362779427683 29.28368393260405 26.783300064789394 3 27.70095186741258 19.10549926232148 21.354669842054417 31.838879028211526 4 26.449145116717965 20.169141821504265 19.995394189939113 33.386318871838654 5 27.252353456604283 20.415721165007827 20.145551087046066 32.18637429134182 6 28.391448920443057 22.938619155676378 22.86410239127667 25.80582953260389 7 25.967594568888693 22.30915844735522 22.669759674073408 29.053487309682684 8 25.164947913156137 23.417922966890593 23.011263639563534 28.405865480389735 9 25.539216787615988 23.667123503111732 23.26645547342485 27.52720423584743 10-11 25.014791016049188 22.31440083279037 23.207665865330608 29.46314228582983 12-13 25.628056497936747 22.299609816741185 22.80269159046485 29.26964209485722 14-15 26.073846487976216 21.928617433178307 22.658245148921193 29.33929092992428 16-17 25.853479071648433 21.669493810240624 22.479629588023396 29.99739753008755 18-19 26.19301714260037 21.905681996899503 22.392943000292075 29.508357860208044 20-21 26.221475806391215 22.07297026069634 22.234360840878622 29.471193092033822 22-23 25.885026998284992 21.59113887079018 22.48749316617613 30.036340964748703 24-25 26.070850839156122 22.024852651523663 22.529432249657372 29.374864259662843 26-27 26.25321096107903 22.10245867876909 22.564163053165277 29.080167306986603 28-29 25.995210706448884 21.690182509904364 22.666015113048296 29.648591670598456 30-31 26.388311595912388 21.955075996719728 22.60345402189095 29.05315838547693 32-33 26.3646835152399 21.96405291862287 22.715408211397815 28.95585535473942 34-35 26.101511200007188 21.63558131944798 22.9284003996331 29.33450708091173 36-37 26.320609190211318 21.910843039234194 22.96968755117175 28.79886021938274 38-39 26.50263485465802 21.834363330280816 23.002144075278427 28.66085773978274 40-41 26.073463579359185 21.56290595655968 23.29212880288227 29.071501661198866 42-43 26.295342377253018 21.93522455889007 23.321649309130184 28.44778375472673 44-45 26.368652475723998 21.9290277072772 23.29270302518903 28.40961679180977 46-47 26.136263823445244 21.525898683319497 23.506586235914465 28.831251257320794 48-49 26.343089749637045 21.84580655832103 23.46134290597453 28.349760786067396 50-51 26.494494274777423 21.753850349366616 23.55888349014091 28.192771885715047 52-53 26.379467412883862 21.458825578989778 23.68967382033442 28.472033187791943 54-55 26.50440617721539 21.865517454359257 23.59391841947445 28.03615794895091 56-57 26.634151490230057 21.845272911984733 23.500204502574974 28.02037109521024 58-59 26.359038419780074 21.645314211683303 23.60204651752886 28.393600851007765 60-61 26.82672716540038 21.844286487618707 23.48188710348711 27.8470992434938 62-63 26.856676578721377 21.753853018388746 23.656600786648372 27.73286961624151 64-65 26.506882232740825 21.462677587882016 23.78234096181839 28.24809921755877 66-67 26.67992987570666 21.822837670140053 23.578702700573206 27.918529753580078 68-69 26.639601519140538 21.955283800199602 23.515970393919826 27.889144286740038 70-71 26.570066011610926 21.653483538976165 23.776743601689816 27.9997068477231 72-73 26.89667452598487 21.70399842813636 23.559534335396403 27.839792710482364 74-75 26.931027455090696 21.795935598451102 23.478760881590823 27.794276064867386 76-77 26.7832039606381 21.635946108777844 23.328123499262183 28.25272643132187 78-79 27.01300365127154 21.971686631221573 23.53895757265176 27.476352144855127 80-81 27.393390191897655 21.496535181236673 23.16631130063966 27.943763326226012 82-83 27.358035745032545 21.1835807018148 23.168841902269364 28.289541650883294 84-85 27.569581926167462 21.39746127867707 22.739024106207058 28.29393268894841 86-87 28.43360396779563 20.65088154363556 22.743486088935693 28.172028399633113 88-89 27.468157454901736 19.895106910264538 22.759495129963693 29.877240504870034 90-91 25.064972567138323 20.84897487727404 25.2526710944268 28.833381461160844 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 0.5 9 0.0 10 1.0 11 2.5 12 4.5 13 9.0 14 13.0 15 21.0 16 32.5 17 43.0 18 61.5 19 89.0 20 132.0 21 213.0 22 298.0 23 397.5 24 537.0 25 690.0 26 902.5 27 1175.0 28 1500.0 29 1877.5 30 2294.0 31 2771.0 32 3327.0 33 3963.5 34 4809.5 35 5740.0 36 6695.0 37 7748.5 38 8963.0 39 10364.5 40 11909.0 41 13678.5 42 15392.5 43 17048.5 44 19030.5 45 21318.5 46 23625.0 47 26021.0 48 28439.0 49 30780.0 50 32992.0 51 35091.5 52 36797.0 53 38095.0 54 39451.5 55 40353.0 56 40587.0 57 40451.5 58 40057.5 59 39587.0 60 38749.5 61 37443.5 62 36247.5 63 34808.5 64 32696.0 65 30471.0 66 28077.0 67 25622.0 68 23129.5 69 20263.0 70 17451.5 71 14977.0 72 12705.5 73 10430.5 74 8262.5 75 6611.5 76 5317.5 77 4121.0 78 3194.0 79 2419.5 80 1774.5 81 1303.0 82 939.5 83 648.5 84 449.0 85 340.5 86 246.5 87 183.5 88 132.5 89 81.0 90 50.5 91 38.0 92 31.0 93 24.5 94 18.0 95 12.5 96 8.5 97 7.0 98 5.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013105963587888592 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05655842013479757 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0010372511461625164 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.140054646486354E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8878.0 32-33 19129.0 34-35 21136.0 36-37 22819.0 38-39 24264.0 40-41 25848.0 42-43 26179.0 44-45 26358.0 46-47 26604.0 48-49 25755.0 50-51 25291.0 52-53 24895.0 54-55 23684.0 56-57 22885.0 58-59 21469.0 60-61 20339.0 62-63 19367.0 64-65 18120.0 66-67 16397.0 68-69 15524.0 70-71 14427.0 72-73 12965.0 74-75 11844.0 76-77 10619.0 78-79 9573.0 80-81 8707.0 82-83 7793.0 84-85 6910.0 86-87 6266.0 88-89 5432.0 90-91 4631.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88254679630856 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88830695032617 99.77098493369986 2 0.10712500195586369 0.213998360418224 3 0.003237826647514539 0.009702071149161174 4 0.001330221070451851 0.005314634732753707 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0026211927175777182 0.0 0.0 0.0 0.0 2 0.0026211927175777182 0.0 0.0 0.0 0.0 3 0.0026211927175777182 0.0 0.0 0.0 0.0 4 0.0026211927175777182 0.0 0.0 0.0 0.0 5 0.00280842076883327 0.0 0.0 0.0 0.0 6 0.00280842076883327 0.0 0.0 0.0 0.0 7 0.0033701049225999236 0.0 0.0 0.0 0.0 8 0.007489122050222052 0.0 0.0 0.0 0.0 9 0.007863578152733156 0.0 0.0 0.0 0.0 10-11 0.007863578152733156 0.0 0.0 0.0 1.8722805125555132E-4 12-13 0.007863578152733156 0.0 0.0 0.0 1.8722805125555132E-4 14-15 0.007863578152733156 0.0 0.0 0.0 2.8084207688332697E-4 16-17 0.007863578152733156 0.0 0.0 0.0 3.7445610251110264E-4 18-19 0.007863578152733156 0.0 0.0 0.0 3.7445610251110264E-4 20-21 0.007863578152733156 0.0 0.0 0.0 3.7445610251110264E-4 22-23 0.007863578152733156 0.0 0.0 0.0 3.7445610251110264E-4 24-25 0.00842526230649981 0.0 0.0 0.0 3.7445610251110264E-4 26-27 0.00861249035775536 0.0 0.0 0.0 3.7445610251110264E-4 28-29 0.008986946460266463 0.0 0.0 0.0 3.7445610251110264E-4 30-31 0.009174174511522014 0.0 0.0 0.0 4.680701281388783E-4 32-33 0.009361402562777565 0.0 0.0 0.0 5.616841537666539E-4 34-35 0.009548630614033117 0.0 0.0 0.0 5.616841537666539E-4 36-37 0.009735858665288668 0.0 1.8722805125555132E-4 0.0 5.616841537666539E-4 38-39 0.010016700742171994 0.0 1.8722805125555132E-4 0.0 5.616841537666539E-4 40-41 0.010297542819055322 0.0 1.8722805125555132E-4 0.0 7.489122050222053E-4 42-43 0.010297542819055322 0.0 1.8722805125555132E-4 0.0 7.489122050222053E-4 44-45 0.010297542819055322 9.361402562777566E-5 1.8722805125555132E-4 0.0 7.489122050222053E-4 46-47 0.010484770870310873 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 48-49 0.010484770870310873 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 50-51 0.01057838489593865 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 52-53 0.010952840998449752 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 54-55 0.011327297100960854 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 56-57 0.011608139177844182 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 58-59 0.011608139177844182 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 60-61 0.011795367229099733 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 62-63 0.012357051382866387 1.8722805125555132E-4 1.8722805125555132E-4 0.0 7.489122050222053E-4 64-65 0.012357051382866387 1.8722805125555132E-4 1.8722805125555132E-4 0.0 9.361402562777565E-4 66-67 0.012357051382866387 1.8722805125555132E-4 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