##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-22A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 598559 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.6187844473143 40.0 40.0 40.0 40.0 40.0 2 39.4317719723536 40.0 40.0 40.0 40.0 40.0 3 39.402444871767024 40.0 40.0 40.0 40.0 40.0 4 39.23337214877731 40.0 40.0 40.0 40.0 40.0 5 39.323542040133056 40.0 40.0 40.0 40.0 40.0 6 39.45218432936436 40.0 40.0 40.0 40.0 40.0 7 39.43716826578499 40.0 40.0 40.0 40.0 40.0 8 39.48563800728082 40.0 40.0 40.0 40.0 40.0 9 39.47411700433875 40.0 40.0 40.0 40.0 40.0 10-11 39.475560471064675 40.0 40.0 40.0 40.0 40.0 12-13 39.39537789925471 40.0 40.0 40.0 40.0 40.0 14-15 39.42917239570368 40.0 40.0 40.0 40.0 40.0 16-17 39.53083655913619 40.0 40.0 40.0 40.0 40.0 18-19 39.564313626559795 40.0 40.0 40.0 40.0 40.0 20-21 39.5457089443146 40.0 40.0 40.0 40.0 40.0 22-23 39.58041229018359 40.0 40.0 40.0 40.0 40.0 24-25 39.55893403991921 40.0 40.0 40.0 40.0 40.0 26-27 39.53338935677185 40.0 40.0 40.0 40.0 40.0 28-29 39.55037013895038 40.0 40.0 40.0 40.0 40.0 30-31 39.52852443860063 40.0 40.0 40.0 40.0 40.0 32-33 39.53152102122453 40.0 40.0 40.0 40.0 40.0 34-35 39.52704966760973 40.0 40.0 40.0 40.0 40.0 36-37 39.50725397144807 40.0 40.0 40.0 40.0 40.0 38-39 39.45475035102622 40.0 40.0 40.0 40.0 40.0 40-41 39.50346414805473 40.0 40.0 40.0 40.0 40.0 42-43 39.480179879373104 40.0 40.0 40.0 40.0 40.0 44-45 39.37570728716484 40.0 40.0 40.0 40.0 40.0 46-47 39.381931104693095 40.0 40.0 40.0 40.0 40.0 48-49 39.449025878027754 40.0 40.0 40.0 40.0 40.0 50-51 39.41406654410106 40.0 40.0 40.0 40.0 40.0 52-53 39.37011361766855 40.0 40.0 40.0 40.0 40.0 54-55 39.29930952584088 40.0 40.0 40.0 40.0 40.0 56-57 39.31499352779605 40.0 40.0 40.0 40.0 40.0 58-59 39.26947947723599 40.0 40.0 40.0 40.0 40.0 60-61 39.19956199690537 40.0 40.0 40.0 40.0 40.0 62-63 39.45719530117471 40.0 40.0 40.0 40.0 40.0 64-65 39.465749355814324 40.0 40.0 40.0 40.0 40.0 66-67 39.4611044054401 40.0 40.0 40.0 40.0 40.0 68-69 39.47065637100876 40.0 40.0 40.0 40.0 40.0 70-71 39.46029558914951 40.0 40.0 40.0 40.0 40.0 72-73 39.46140118892029 40.0 40.0 40.0 40.0 40.0 74-75 39.46373881875323 40.0 40.0 40.0 40.0 40.0 76-77 39.46766593574361 40.0 40.0 40.0 40.0 40.0 78-79 39.44069489378522 40.0 40.0 40.0 40.0 40.0 80-81 39.437664209410734 40.0 40.0 40.0 40.0 40.0 82-83 39.45013143200393 40.0 40.0 40.0 40.0 40.0 84-85 39.410237460398946 40.0 40.0 40.0 40.0 40.0 86-87 39.438072981805824 40.0 40.0 40.0 40.0 40.0 88-89 39.46510277301555 40.0 40.0 40.0 40.0 40.0 90-91 39.51678812059221 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013031104566387341 1101 2 -0.054586624018220675 1101 3 0.017219397297289163 1101 4 -0.1803634830730516 1101 5 -0.1052011830342039 1101 6 0.0011729559381663535 1101 7 -0.08028009979901896 1101 8 -0.0828698021091725 1101 9 0.004612265763476842 1101 10-11 -0.024938306130252386 1101 12-13 -0.1623802672495529 1101 14-15 -0.021878061271813465 1101 16-17 0.010067144121371996 1101 18-19 -0.013255182365590201 1101 20-21 0.029071523824306666 1101 22-23 0.01823741897467812 1101 24-25 0.03535963796026209 1101 26-27 0.028101365860763394 1101 28-29 0.048428460235754756 1101 30-31 0.020401306718895285 1101 32-33 -0.029238284368766188 1101 34-35 -0.060856399299289876 1101 36-37 -0.17586608241498425 1101 38-39 0.03382066061897149 1101 40-41 -0.12212626290178719 1101 42-43 0.05451218041943662 1101 44-45 -0.05844269832004301 1101 46-47 0.06187358531821019 1101 48-49 -0.07424049822516565 1101 50-51 0.061697669146397516 1101 52-53 0.028370369672991558 1101 54-55 -0.056386971935374675 1101 56-57 0.06538502861808837 1101 58-59 0.07379328656426054 1101 60-61 -0.1530007332408161 1101 62-63 0.13648429119123762 1101 64-65 0.02134835485158959 1101 66-67 0.19260545707154364 1101 68-69 0.019859443653174935 1101 70-71 -0.06804509283895044 1101 72-73 0.03493202403966933 1101 74-75 -0.14820607592511692 1101 76-77 -0.22329705201016026 1101 78-79 -0.12823297517077492 1101 80-81 0.03410636097225961 1101 82-83 -0.016152686014216044 1101 84-85 -0.27568467038895506 1101 86-87 -0.5604915607634169 1101 88-89 -0.5272873172429087 1101 90-91 0.2962380482493856 1102 1 0.025143615669641406 1102 2 0.16082424220220304 1102 3 0.06342254635929834 1102 4 0.14244639045046625 1102 5 -0.008429254501294281 1102 6 -0.05087375712525244 1102 7 -0.09948096583537591 1102 8 -0.10544782820888088 1102 9 -0.26501517635476546 1102 10-11 0.007506699129095296 1102 12-13 -0.08821840488961641 1102 14-15 -0.10036056159732709 1102 16-17 0.017551568896386982 1102 18-19 0.0595385006953606 1102 20-21 -0.01756996070817962 1102 22-23 0.011177013892705645 1102 24-25 -0.07730825713645118 1102 26-27 -0.08209603507576446 1102 28-29 -0.03218504581403181 1102 30-31 -0.05787122887115004 1102 32-33 0.03621903821812822 1102 34-35 -0.10460032745989878 1102 36-37 -0.13535548978929768 1102 38-39 -0.040571800400272195 1102 40-41 -0.08919133187904293 1102 42-43 -0.01348336790228899 1102 44-45 -0.046938529665247586 1102 46-47 0.09059236898555412 1102 48-49 -0.17555047992901507 1102 50-51 -0.19184250461243835 1102 52-53 0.04311006416368457 1102 54-55 -0.1432506102883977 1102 56-57 -0.07643071913271626 1102 58-59 -0.31592622676084403 1102 60-61 0.029362463754232238 1102 62-63 -0.21910342057388732 1102 64-65 -0.2736859619829701 1102 66-67 -0.17447832343992076 1102 68-69 -0.27905563459258076 1102 70-71 0.006363250760024641 1102 72-73 -0.11277086650605384 1102 74-75 -0.13244471035448413 1102 76-77 0.026356423196652656 1102 78-79 0.018357453883488972 1102 80-81 -0.01932494770201032 1102 82-83 -0.27813707843339586 1102 84-85 0.22209803471081813 1102 86-87 -0.2080153980590893 1102 88-89 0.062060428507273 1102 90-91 0.2962380482493856 1103 1 0.11473618287476484 1103 2 0.03551904944286122 1103 3 0.05802149226833109 1103 4 -0.22660055223564513 1103 5 0.06699661344883623 1103 6 -0.02066327096397913 1103 7 -0.036405213100358935 1103 8 0.12778326385917183 1103 9 0.030797309672223605 1103 10-11 0.011882143221910724 1103 12-13 -0.08186921493705057 1103 14-15 -0.10795671622389591 1103 16-17 0.022222890405942053 1103 18-19 0.05541982802654388 1103 20-21 -0.05697920934924383 1103 22-23 -0.06121260575355336 1103 24-25 0.04150312728209826 1103 26-27 0.10832063558758875 1103 28-29 -0.042552560982358045 1103 30-31 -3.7877891021764754E-4 1103 32-33 0.052865699668522836 1103 34-35 0.011560294361032675 1103 36-37 0.056243682005195694 1103 38-39 0.11104777093505902 1103 40-41 0.044110452129913824 1103 42-43 0.15215486025465452 1103 44-45 -0.015979261644041287 1103 46-47 -2.4385461742326697E-5 1103 48-49 0.05630336105772926 1103 50-51 0.014044513176841633 1103 52-53 0.04508217040767448 1103 54-55 -0.05909693741998723 1103 56-57 0.0013419946150960982 1103 58-59 0.07257136928610208 1103 60-61 -0.09843670290842965 1103 62-63 0.24841838343141376 1103 64-65 0.09681929468332129 1103 66-67 -0.004036099648594416 1103 68-69 0.09235247705436223 1103 70-71 0.21613108087235133 1103 72-73 0.1909139973393934 1103 74-75 0.3310486414775369 1103 76-77 0.08711553791920323 1103 78-79 0.11232624696073401 1103 80-81 0.05417866155303841 1103 82-83 0.16968036833238642 1103 84-85 0.30223740753312 1103 86-87 0.4864127544335801 1103 88-89 0.3133227306039785 1103 90-91 0.2962380482493856 1104 1 0.05803339993583734 1104 2 0.0543770656304261 1104 3 0.054928727375759934 1104 4 -0.06359771301467276 1104 5 0.09066018970550971 1104 6 0.14160170049074594 1104 7 -0.15635582305796447 1104 8 0.056170842247809105 1104 9 -0.006235802536274093 1104 10-11 0.0026909797378920075 1104 12-13 -0.04284699962252603 1104 14-15 -0.04390272942231377 1104 16-17 -0.020238259678713177 1104 18-19 -0.12545197058893365 1104 20-21 0.06770552879796554 1104 22-23 -0.046585554736275014 1104 24-25 -0.13801146562290967 1104 26-27 -0.15419230653454008 1104 28-29 -0.06818718613375552 1104 30-31 0.03506556270371419 1104 32-33 -0.07678432868620888 1104 34-35 -0.030374186906122702 1104 36-37 -0.10818244155481693 1104 38-39 0.04704167450994845 1104 40-41 -0.06315607471448459 1104 42-43 -0.10976371480053615 1104 44-45 0.06050011709168501 1104 46-47 -0.03809558795808243 1104 48-49 -0.005557569729646161 1104 50-51 -0.0717448033710042 1104 52-53 0.009594148457523488 1104 54-55 0.09873486486866767 1104 56-57 -0.12115598521509696 1104 58-59 0.012521420110680026 1104 60-61 -0.055621348970532836 1104 62-63 -0.08383720361614166 1104 64-65 -0.034212198427560736 1104 66-67 0.09568747106604292 1104 68-69 0.17886667274925117 1104 70-71 0.20350456728342436 1104 72-73 -0.08582197467453057 1104 74-75 0.012967181692104646 1104 76-77 0.19530740433762617 1104 78-79 0.06708919318317896 1104 80-81 0.5032930301589289 1104 82-83 -0.31331078976204907 1104 84-85 -0.09522684132431891 1104 86-87 0.2959365639573903 1104 88-89 0.5238490463934511 1104 90-91 -1.3037619517506158 1201 1 -0.05644389595075694 1201 2 0.09540159840908302 1201 3 0.020642567152570734 1201 4 0.08519575811762792 1201 5 0.02878422078305931 1201 6 0.09782779164937239 1201 7 -0.0034622998495947854 1201 8 0.04651801100577302 1201 9 -0.036842120998663574 1201 10-11 -0.04932355353601281 1201 12-13 -0.01680746683636869 1201 14-15 0.07492224247612 1201 16-17 0.02283029613347054 1201 18-19 -6.435039513874585E-4 1201 20-21 0.0557115257994667 1201 22-23 0.06203731100802656 1201 24-25 -0.006155471697951498 1201 26-27 0.018071411456318742 1201 28-29 0.052078506845575134 1201 30-31 0.07648846153124822 1201 32-33 0.00449669873324865 1201 34-35 0.024041869501793656 1201 36-37 0.13451777207929894 1201 38-39 0.13252590452484725 1201 40-41 0.09468939041855151 1201 42-43 0.03319435169343876 1201 44-45 -0.08522254031805687 1201 46-47 0.027971946811575776 1201 48-49 0.20094287719432202 1201 50-51 0.2250150536276081 1201 52-53 0.04209812278463687 1201 54-55 0.2312795426967611 1201 56-57 0.12407211282270225 1201 58-59 0.06334055641121239 1201 60-61 0.2549813871173967 1201 62-63 0.13727501065152836 1201 64-65 0.022276607283906458 1201 66-67 0.2811326390416511 1201 68-69 0.14092523535220636 1201 70-71 0.051783462042628514 1201 72-73 0.19976061378200427 1201 74-75 0.1422081772594055 1201 76-77 0.22906383398917285 1201 78-79 0.21459886436306874 1201 80-81 0.1737315516144946 1201 82-83 0.2446000021578172 1201 84-85 0.4450945503902659 1201 86-87 0.4864127544335801 1201 88-89 0.5238490463934511 1201 90-91 0.2962380482493856 1202 1 0.011880451919481061 1202 2 0.05181958836822531 1202 3 0.05291665333207618 1202 4 -0.15764058234741896 1202 5 -0.09655066461825612 1202 6 -0.006053825395511581 1202 7 0.017678985183223972 1202 8 0.11533829463098755 1202 9 0.019055872227475845 1202 10-11 0.11587014469517243 1202 12-13 -0.09883479922443428 1202 14-15 -0.011841497114929211 1202 16-17 -0.027879502718228366 1202 18-19 -0.003084920620558762 1202 20-21 0.01996515352536221 1202 22-23 -0.0012930962552957226 1202 24-25 0.001206304605595676 1202 26-27 0.04485267803502779 1202 28-29 -0.03805965614657225 1202 30-31 0.015893280063288273 1202 32-33 0.025903382178618983 1202 34-35 -0.0010673450832356934 1202 36-37 -0.18689911976720452 1202 38-39 -0.11438954689785419 1202 40-41 -0.20208238361260555 1202 42-43 -0.1271509440268943 1202 44-45 -0.05339629731398077 1202 46-47 -0.07106285243681043 1202 48-49 -0.047778386760029434 1202 50-51 -0.07191333216519524 1202 52-53 -0.07706033083752573 1202 54-55 -0.24136724461192216 1202 56-57 -0.04630107122498828 1202 58-59 -0.18645669728089587 1202 60-61 -0.03335436233572864 1202 62-63 -0.05867773611882399 1202 64-65 -0.001877711684876715 1202 66-67 -0.014408777292032937 1202 68-69 0.017981579715495855 1202 70-71 -0.2441953604328191 1202 72-73 0.025586047223683295 1202 74-75 -0.2504644644416345 1202 76-77 -0.0981292691606157 1202 78-79 0.259989937522775 1202 80-81 -0.09081777149904013 1202 82-83 0.025175227264917055 1202 84-85 -0.17366749715739616 1202 86-87 -0.6102056030543395 1202 88-89 -1.20579765099518 1202 90-91 -0.2037619517506144 1203 1 0.019245604291171503 1203 2 0.17136758997609292 1203 3 -0.012259900532505696 1203 4 0.07088733204449227 1203 5 0.08995792645804812 1203 6 0.07460252034226045 1203 7 0.09337878676131339 1203 8 -0.036498509350899155 1203 9 0.03400878105428262 1203 10-11 0.002000148682832048 1203 12-13 -0.027072207280141924 1203 14-15 0.040982932321981025 1203 16-17 -0.029841301295164158 1203 18-19 0.03879107128881998 1203 20-21 0.05187520866392248 1203 22-23 0.012925539389868845 1203 24-25 -0.0952893626221254 1203 26-27 -0.027134920195024392 1203 28-29 -0.021537308458214 1203 30-31 -0.04221279158318936 1203 32-33 0.04278151363331517 1203 34-35 0.14539623103691213 1203 36-37 0.0123560940124392 1203 38-39 -0.026443306478455497 1203 40-41 0.03353380635351755 1203 42-43 -0.028909001528049316 1203 44-45 0.03706311244079785 1203 46-47 0.1477521169227316 1203 48-49 -0.022109059500486694 1203 50-51 -0.005694874087495805 1203 52-53 0.09016136765178118 1203 54-55 -0.006946674364279204 1203 56-57 0.21817437191116795 1203 58-59 0.1963931368362566 1203 60-61 0.1341737728212209 1203 62-63 0.07422038703089129 1203 64-65 -0.008835569038787128 1203 66-67 -0.1074156717525625 1203 68-69 0.2128545540286737 1203 70-71 -0.1556913620926892 1203 72-73 0.12551517634374676 1203 74-75 0.11246230829188875 1203 76-77 -0.032789131979477304 1203 78-79 0.13447096752697973 1203 80-81 0.1534541156141387 1203 82-83 0.04698770302704958 1203 84-85 0.3657294710251833 1203 86-87 0.33826460628542776 1203 88-89 0.5238490463934511 1203 90-91 0.2962380482493856 1204 1 -0.060431239272340065 1204 2 -0.36971297268375025 1204 3 -0.14054341502525602 1204 4 -0.19965294928542932 1204 5 -0.24819078411314166 1204 6 -0.18378942339175808 1204 7 -0.15194570172973698 1204 8 -0.025875374227283032 1204 9 -0.09729183701902855 1204 10-11 -0.15759996853797276 1204 12-13 -0.12092211137220943 1204 14-15 -0.024750630124742656 1204 16-17 -0.061440965617414633 1204 18-19 2.913643408248845E-4 1204 20-21 0.0017033736255527288 1204 22-23 -0.05170907804406255 1204 24-25 -0.09357101223849185 1204 26-27 -0.05082884069800997 1204 28-29 -0.10047486174039477 1204 30-31 -0.16737596826691004 1204 32-33 -0.12470306826225652 1204 34-35 -0.05932052532040899 1204 36-37 -0.20588390181423932 1204 38-39 -0.15891949497333258 1204 40-41 -0.180264784614792 1204 42-43 -0.2116893385538532 1204 44-45 -0.21160280923389507 1204 46-47 -0.03325773325842363 1204 48-49 -0.03652082085276476 1204 50-51 -0.12062374043523505 1204 52-53 -0.06297290938671551 1204 54-55 -0.28234731604447205 1204 56-57 0.04890457401747028 1204 58-59 -0.08362590564441774 1204 60-61 -0.1265820005771161 1204 62-63 -0.32027998907855704 1204 64-65 -0.197453149511297 1204 66-67 0.02149121044735125 1204 68-69 -0.1718373101939079 1204 70-71 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0.12489373144258309 2401 40-41 0.02920943359777084 2401 42-43 0.05214663686862764 2401 44-45 0.046173035846081234 2401 46-47 -0.06583899831774431 2401 48-49 0.054016797236442926 2401 50-51 -0.08300409525739383 2401 52-53 -0.01931930045267194 2401 54-55 0.03844232583951879 2401 56-57 -0.07032362137205439 2401 58-59 -0.0446006752491499 2401 60-61 0.10314574330102744 2401 62-63 0.10947168160492282 2401 64-65 0.11636106149585146 2401 66-67 -0.02907741863977975 2401 68-69 0.0021708795782586776 2401 70-71 -0.08227068266435111 2401 72-73 0.171826386330153 2401 74-75 0.16826855458405277 2401 76-77 -0.058940401383487995 2401 78-79 0.09563221878502759 2401 80-81 0.11462835470180721 2401 82-83 0.17703862559814354 2401 84-85 -0.2784244868542629 2401 86-87 -0.6832842152633916 2401 88-89 0.12384904639345251 2401 90-91 -0.051588038707137684 2402 1 0.005275098806464484 2402 2 0.11222188495214169 2402 3 0.03905870246108378 2402 4 0.06849308177582714 2402 5 0.011702352930285542 2402 6 0.03452917022342916 2402 7 0.0783579005135735 2402 8 0.13188679502781753 2402 9 0.051404057430204375 2402 10-11 0.11841698213790153 2402 12-13 0.09581241192373113 2402 14-15 0.07852963123562517 2402 16-17 0.09848609974064715 2402 18-19 0.07497073053293946 2402 20-21 0.00956979942365166 2402 22-23 0.060259977535459086 2402 24-25 0.04433073495736295 2402 26-27 0.09003770193932326 2402 28-29 0.0788733551132097 2402 30-31 0.027566915325273555 2402 32-33 0.033366991363962484 2402 34-35 0.11532929555615112 2402 36-37 0.08966744184723296 2402 38-39 -0.28332201984012784 2402 40-41 0.10502075506947506 2402 42-43 0.11145393901007594 2402 44-45 0.18952855650836398 2402 46-47 0.09772908915545031 2402 48-49 0.019172206515897017 2402 50-51 0.1608758124407501 2402 52-53 0.15776997390509706 2402 54-55 0.1096242077611791 2402 56-57 0.1530274466841135 2402 58-59 -0.03011915503098095 2402 60-61 0.09729923968350818 2402 62-63 0.11797230781451162 2402 64-65 0.052088106146207735 2402 66-67 0.1105051573580127 2402 68-69 0.15827950649401856 2402 70-71 0.1606755397798807 2402 72-73 -0.03516711691822394 2402 74-75 0.15180197947639584 2402 76-77 -0.0549961435225228 2402 78-79 0.07901490188308458 2402 80-81 0.2814515103041657 2402 82-83 -0.20820472092164977 2402 84-85 0.09193790311596928 2402 86-87 0.2786205466413705 2402 88-89 0.15068342794481282 2402 90-91 -5.8248046912012796E-5 2403 1 0.0025463414658375427 2403 2 0.07470744254116113 2403 3 0.09470564783517688 2403 4 -0.016370077313112574 2403 5 0.1173386997392285 2403 6 0.09897441743868995 2403 7 0.08578137439482703 2403 8 0.10145846371965916 2403 9 0.15052751130853892 2403 10-11 0.11904543226940945 2403 12-13 0.11694012911222273 2403 14-15 0.030527087579990564 2403 16-17 0.0550842290192648 2403 18-19 -0.0323688504804025 2403 20-21 0.08196397051136728 2403 22-23 0.06106755834578337 2403 24-25 0.06922094277880575 2403 26-27 0.024128807802568986 2403 28-29 0.01129705290781402 2403 30-31 0.026427196381305862 2403 32-33 0.049189058105305605 2403 34-35 -0.04345124976284609 2403 36-37 0.03817892952347535 2403 38-39 0.09121990739515695 2403 40-41 -0.0473525105112671 2403 42-43 -0.014514848149573822 2403 44-45 -0.01747944073763108 2403 46-47 0.019208016743718304 2403 48-49 0.054636661857685453 2403 50-51 0.13991950954707733 2403 52-53 0.24963287183263105 2403 54-55 -0.013286452893858325 2403 56-57 0.1124450400363628 2403 58-59 0.03867885460795861 2403 60-61 0.0181954257455601 2403 62-63 0.07825127493205741 2403 64-65 -0.04393697452126588 2403 66-67 0.06652318908875543 2403 68-69 -0.029800668861724944 2403 70-71 0.007614998123095518 2403 72-73 0.1133948029645282 2403 74-75 0.1339778851831923 2403 76-77 0.19265628124174583 2403 78-79 -0.09035623495189782 2403 80-81 -0.013521054641387309 2403 82-83 0.04889433592935433 2403 84-85 -0.11999351223204258 2403 86-87 0.4864127544335801 2403 88-89 0.02755275009715774 2403 90-91 0.2962380482493856 2404 1 -0.04092223248302673 2404 2 -0.04631722147919248 2404 3 0.009488752079427343 2404 4 0.13834803625990588 2404 5 0.06514761382816658 2404 6 -0.058994745336569565 2404 7 0.11491122993911773 2404 8 0.11887317483097348 2404 9 0.07046303595512882 2404 10-11 0.01204044429601936 2404 12-13 0.11754221736191539 2404 14-15 0.07704285706317648 2404 16-17 0.1082627548162236 2404 18-19 0.0773127588874658 2404 20-21 -6.40718577692212E-4 2404 22-23 0.01801654339702452 2404 24-25 0.08974726415222278 2404 26-27 0.022630218106193922 2404 28-29 0.09681215096217954 2404 30-31 0.10055377056357884 2404 32-33 0.06138549852179409 2404 34-35 0.08284398155597472 2404 36-37 0.023108347030976972 2404 38-39 0.1448044373934252 2404 40-41 0.05240552998060366 2404 42-43 0.12195012200371735 2404 44-45 0.022415847343225437 2404 46-47 -0.0017269247578681757 2404 48-49 0.20872639710766094 2404 50-51 0.13547794527731583 2404 52-53 0.1173865461580661 2404 54-55 0.09700790908588175 2404 56-57 0.018725735271075905 2404 58-59 0.17244630116407933 2404 60-61 -0.10763370427534369 2404 62-63 0.11585577740829933 2404 64-65 0.2203593307571765 2404 66-67 0.11440283072708723 2404 68-69 0.11782693698216917 2404 70-71 0.073753837535385 2404 72-73 0.20932172134786242 2404 74-75 0.09128535167275231 2404 76-77 0.304787916669774 2404 78-79 0.3924599217453988 2404 80-81 0.2329186897037303 2404 82-83 0.16924220364896314 2404 84-85 0.41245421062932763 2404 86-87 0.379746087766911 2404 88-89 0.5238490463934511 2404 90-91 0.2962380482493856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 278.0 32 1841.0 33 3365.0 34 4745.0 35 5899.0 36 8950.0 37 13514.0 38 37209.0 39 246680.0 40 276067.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.27971344512404 10.307254589773104 33.654159406173825 30.758872558929028 2 27.223451253500514 16.782904806143524 29.405297384725404 26.588346555630558 3 27.747974719284148 19.152999119552124 21.41175723696411 31.687268924199618 4 26.450692412945088 20.017074340207063 20.308106636104377 33.22412661074347 5 27.265649668620807 20.35755873689979 20.40066225718768 31.97612933729173 6 28.249512579378138 22.719063617788724 23.193035273047435 25.8383885297857 7 25.92676745316669 22.146521896755374 22.945774769070386 28.980935881007554 8 25.140044673958624 23.430939974171302 23.356761822978186 28.07225352889189 9 25.62136731717341 23.548555781468494 23.508960687250546 27.321116214107548 10-11 24.85803404509831 22.269149741295344 23.65322382588851 29.219592387717835 12-13 25.53624621800023 22.180603750006263 23.254265661363373 29.02888437063013 14-15 25.89218439619152 21.94946529916015 23.17231885244395 28.986031452204376 16-17 25.698552690712194 21.55835932631537 23.097723031480605 29.645364951491832 18-19 26.058584032651748 21.837362732830012 22.898828686896362 29.20522454762187 20-21 26.153395070494305 21.960157645278073 22.826655350600358 29.05979193362726 22-23 25.773148511675544 21.553096687210452 23.100646719872227 29.573108081241784 24-25 25.94639793236757 21.87871204008293 23.08293752161441 29.091952505935087 26-27 26.167679376636222 21.98145880355988 23.119024189762413 28.731837630041486 28-29 25.94071762349242 21.565877382179536 23.235470521702954 29.257934472625085 30-31 26.26284142871702 21.79847665445384 23.237869454733183 28.70081246209596 32-33 26.300756662046286 21.86519294582156 23.19121596542345 28.642834426708703 34-35 26.049210109177697 21.488325288973588 23.41567670579016 29.04678789605856 36-37 26.313993015350416 21.761229149518936 23.44638049134359 28.478397343787055 38-39 26.504893010920405 21.783506636638077 23.414477679518786 28.297122672922733 40-41 26.117266803663814 21.430415431799172 23.635208955069757 28.817108809467264 42-43 26.231266671346344 21.78868191071178 23.672215007721466 28.30783641022041 44-45 26.422072569117493 21.716442675603638 23.640017964626573 28.221466790652293 46-47 26.134149350224313 21.502137386899754 23.796114940561008 28.567598322314925 48-49 26.38012194607769 21.75389633087292 23.70508797771621 28.160893745333176 50-51 26.50476246820091 21.65517955392534 23.757173282849198 28.08288469502455 52-53 26.377229630971012 21.39443287501132 23.82002561084451 28.40831188317316 54-55 26.660523333695053 21.591206257660637 23.849414811955306 27.898855596689003 56-57 26.83412960704727 21.70812984661667 23.568720090643637 27.889020455692425 58-59 26.58419822061653 21.424727023923403 23.879387173883202 28.111687581576867 60-61 27.103593359662238 21.65438687905099 23.64001149251769 27.602008268769083 62-63 26.89225899028403 21.72948637902139 23.833152596584622 27.545102034109952 64-65 26.650491002674254 21.44298078442674 23.916741812232146 27.989786400666862 66-67 26.76340627098872 21.691470182756547 23.78627735162077 27.758846194633957 68-69 26.736750708008405 21.597299424667092 23.729845090194996 27.9361047771295 70-71 26.701140355821074 21.418305810742265 23.937609197993577 27.942944635443084 72-73 26.976297596839682 21.655876220783497 23.66289915505322 27.704927027323606 74-75 27.03963762849133 21.66247014847887 23.65148998027204 27.646402242757762 76-77 26.92771975732654 21.41382989494015 23.702717226233034 27.955733121500277 78-79 26.903659674991903 21.856127717132463 23.726924615648397 27.513287992227237 80-81 27.105062627994474 21.3840072391294 23.484307281992667 28.02662285088346 82-83 27.701351248186885 20.775631727612794 23.447591419192303 28.075425605008014 84-85 27.631281006196097 20.757940673953765 23.063463636550775 28.54731468329937 86-87 28.75352408373823 20.400695819087037 22.401175694319477 28.444604402855255 88-89 27.523213564796123 19.156237383932176 23.264029067420267 30.05651998385143 90-91 25.08604206500956 19.592096876991715 26.88336520076482 28.43849585723391 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 4.0 11 5.0 12 7.0 13 12.0 14 22.5 15 35.5 16 46.5 17 60.0 18 81.0 19 121.5 20 181.0 21 257.0 22 358.5 23 487.0 24 641.0 25 830.0 26 1101.0 27 1434.5 28 1778.0 29 2228.0 30 2782.5 31 3379.0 32 4064.5 33 4765.0 34 5641.5 35 6758.5 36 8016.0 37 9273.0 38 10734.0 39 12556.0 40 14304.0 41 16107.0 42 18073.0 43 20207.5 44 22555.0 45 25071.5 46 27707.5 47 30384.5 48 32890.5 49 35186.5 50 37520.0 51 39770.5 52 41551.0 53 42994.5 54 44199.5 55 44941.0 56 45178.5 57 44896.5 58 44426.0 59 43697.5 60 42633.0 61 41261.0 62 39689.0 63 37768.5 64 35378.0 65 32923.0 66 30145.5 67 27340.0 68 24584.5 69 21615.0 70 18734.5 71 16061.0 72 13501.0 73 11019.5 74 8835.0 75 7163.5 76 5656.5 77 4220.5 78 3230.5 79 2472.5 80 1831.5 81 1371.0 82 1010.5 83 707.5 84 503.5 85 379.0 86 269.5 87 175.0 88 126.0 89 91.0 90 64.5 91 49.0 92 33.0 93 20.5 94 15.0 95 12.0 96 8.0 97 5.5 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01253009310694518 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05473341406511908 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.7384280875092083E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011906321065853862 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.005046172478175305 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10002.0 32-33 21404.0 34-35 23342.0 36-37 25454.0 38-39 27110.0 40-41 28285.0 42-43 29159.0 44-45 29266.0 46-47 29694.0 48-49 29390.0 50-51 29070.0 52-53 28507.0 54-55 26741.0 56-57 25483.0 58-59 24496.0 60-61 22997.0 62-63 21681.0 64-65 20275.0 66-67 18596.0 68-69 17132.0 70-71 15728.0 72-73 14296.0 74-75 13215.0 76-77 12063.0 78-79 10677.0 80-81 9538.0 82-83 8658.0 84-85 7804.0 86-87 6990.0 88-89 6208.0 90-91 5298.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.91851295393433 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92225961845487 99.84083592072972 2 0.07424960276854502 0.14837819792106688 3 0.00316843749312738 0.009497566881023382 4 3.2234128343769743E-4 0.0012883144681902956 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010024074485556144 0.0 0.0 0.0 1.670679080926024E-4 2 0.0010024074485556144 0.0 0.0 0.0 1.670679080926024E-4 3 0.0013365432647408193 0.0 0.0 0.0 1.670679080926024E-4 4 0.0013365432647408193 0.0 0.0 0.0 1.670679080926024E-4 5 0.0013365432647408193 0.0 0.0 0.0 1.670679080926024E-4 6 0.001670679080926024 1.670679080926024E-4 0.0 0.0 1.670679080926024E-4 7 0.0020048148971112288 1.670679080926024E-4 0.0 0.0 1.670679080926024E-4 8 0.007016852139889301 1.670679080926024E-4 0.0 0.0 1.670679080926024E-4 9 0.007183920047981903 1.670679080926024E-4 0.0 0.0 1.670679080926024E-4 10-11 0.007350987956074506 1.670679080926024E-4 0.0 0.0 3.341358161852048E-4 12-13 0.007434521910120806 1.670679080926024E-4 0.0 0.0 3.341358161852048E-4 14-15 0.007518055864167108 1.670679080926024E-4 0.0 0.0 3.341358161852048E-4 16-17 0.007518055864167108 1.670679080926024E-4 0.0 0.0 3.341358161852048E-4 18-19 0.007518055864167108 1.670679080926024E-4 0.0 0.0 3.341358161852048E-4 20-21 0.007518055864167108 1.670679080926024E-4 0.0 0.0 5.012037242778072E-4 22-23 0.007518055864167108 1.670679080926024E-4 0.0 0.0 5.012037242778072E-4 24-25 0.007518055864167108 1.670679080926024E-4 0.0 0.0 5.847376783241084E-4 26-27 0.0076851237722597105 1.670679080926024E-4 0.0 0.0 6.682716323704096E-4 28-29 0.008186327496537518 1.670679080926024E-4 0.0 0.0 6.682716323704096E-4 30-31 0.008520463312722722 1.670679080926024E-4 0.0 0.0 7.518055864167108E-4 32-33 0.008771065174861627 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 34-35 0.009439336807232034 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 36-37 0.010024074485556145 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 38-39 0.010107608439602445 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 40-41 0.010358210301741349 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 42-43 0.010358210301741349 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 44-45 0.010608812163880252 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 46-47 0.010859414026019157 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 48-49 0.011026481934111758 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 50-51 0.01119354984220436 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 52-53 0.01177828752052847 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 54-55 0.01186182147457477 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 56-57 0.012195957290759975 3.341358161852048E-4 0.0 0.0 8.35339540463012E-4 58-59 0.012780694969084083 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