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"TTCTC":86826, "TTCTG":65938, "TTCCA":62684, "TTCCT":92639, "TTCCC":63342, "TTCCG":54887, "TTCGA":46287, "TTCGT":42684, "TTCGC":49100, "TTCGG":53568, "TTGAA":48445, "TTGAT":52706, "TTGAC":38543, "TTGAG":51430, "TTGTA":25302, "TTGTT":59437, "TTGTC":45404, "TTGTG":42612, "TTGCA":35291, "TTGCT":55872, "TTGCC":63199, "TTGCG":38543, "TTGGA":51046, "TTGGT":64680, "TTGGC":61669, "TTGGG":58746, "TCAAA":46214, "TCAAT":37027, "TCAAC":48163, "TCAAG":51492, "TCATA":24633, "TCATT":37042, "TCATC":68344, "TCATG":38447, "TCACA":28192, "TCACT":79809, "TCACC":66119, "TCACG":33172, "TCAGA":61418, "TCAGT":26270, "TCAGC":48951, "TCAGG":44347, "TCTAA":15083, "TCTAT":25565, "TCTAC":28497, "TCTAG":21782, "TCTTA":21707, "TCTTT":74769, "TCTTC":103665, "TCTTG":60826, "TCTCA":38555, "TCTCT":61181, "TCTCC":97130, "TCTCG":50960, "TCTGA":253692, "TCTGT":32055, "TCTGC":62600, "TCTGG":64032, "TCCAA":53560, "TCCAT":47633, "TCCAC":55780, "TCCAG":187492, "TCCTA":25027, "TCCTT":78723, "TCCTC":132941, "TCCTG":99933, 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"CTCGC":51294, "CTCGG":50614, "CTGAA":248786, "CTGAT":41917, "CTGAC":32510, "CTGAG":40655, "CTGTA":19911, "CTGTT":51638, "CTGTC":34148, "CTGTG":33129, "CTGCA":51747, "CTGCT":113764, "CTGCC":78571, "CTGCG":47958, "CTGGA":65549, "CTGGT":64631, "CTGGC":63483, "CTGGG":64696, "CCAAA":69599, "CCAAT":42529, "CCAAC":81233, "CCAAG":69910, "CCATA":32682, "CCATT":42968, "CCATC":70148, "CCATG":45486, "CCACA":69003, "CCACT":35836, "CCACC":137536, "CCACG":48431, "CCAGA":118063, "CCAGT":144593, "CCAGC":94395, "CCAGG":61447, "CCTAA":19135, "CCTAT":23705, "CCTAC":28240, "CCTAG":30820, "CCTTA":24519, "CCTTT":59349, "CCTTC":86780, "CCTTG":61438, "CCTCA":57093, "CCTCT":61031, "CCTCC":148044, "CCTCG":66310, "CCTGA":50916, "CCTGT":49222, "CCTGC":106947, "CCTGG":63745, "CCCAA":76102, "CCCAT":47416, "CCCAC":81641, "CCCAG":82226, "CCCTA":26675, "CCCTT":48788, "CCCTC":70017, "CCCTG":56636, "CCCCA":89310, "CCCCT":59782, "CCCCC":77326, "CCCCG":62746, "CCCGA":54014, "CCCGT":36513, "CCCGC":75239, "CCCGG":57479, "CCGAA":53541, "CCGAT":46825, "CCGAC":51724, "CCGAG":59923, "CCGTA":24902, "CCGTT":39514, "CCGTC":54097, "CCGTG":35384, "CCGCA":59287, "CCGCT":58756, "CCGCC":127973, "CCGCG":56096, "CCGGA":56569, "CCGGT":51028, "CCGGC":82013, "CCGGG":53780, "CGAAA":46855, "CGAAT":32957, "CGAAC":39336, "CGAAG":65316, "CGATA":31722, "CGATT":39263, "CGATC":52258, "CGATG":56729, "CGACA":43573, "CGACT":31912, "CGACC":59504, "CGACG":57042, "CGAGA":76743, "CGAGT":25222, "CGAGC":46877, "CGAGG":57664, "CGTAA":16576, "CGTAT":16058, "CGTAC":14978, "CGTAG":33144, "CGTTA":15668, "CGTTT":35297, "CGTTC":40135, "CGTTG":38483, "CGTCA":39537, "CGTCT":296025, "CGTCC":41940, "CGTCG":56805, "CGTGA":30370, "CGTGT":20751, "CGTGC":28688, "CGTGG":41676, "CGCAA":39160, "CGCAT":28604, "CGCAC":39778, "CGCAG":58077, "CGCTA":19831, "CGCTT":42342, "CGCTC":48523, "CGCTG":56258, "CGCCA":75266, "CGCCT":72714, "CGCCC":70708, "CGCCG":102757, "CGCGA":41416, "CGCGT":24810, "CGCGC":47450, "CGCGG":53553, "CGGAA":534364, "CGGAT":41194, "CGGAC":27765, "CGGAG":69284, "CGGTA":29890, "CGGTT":43969, "CGGTC":46879, "CGGTG":57060, "CGGCA":68519, "CGGCT":57736, "CGGCC":70513, "CGGCG":95375, "CGGGA":50695, "CGGGT":39366, "CGGGC":50907, "CGGGG":51673, "GAAAA":68225, "GAAAT":45698, "GAAAC":47118, "GAAAG":58840, "GAATA":30434, "GAATT":37250, "GAATC":39597, "GAATG":36092, "GAACA":46845, "GAACT":209716, "GAACC":51184, "GAACG":35936, "GAAGA":571343, "GAAGT":37124, "GAAGC":65594, "GAAGG":63744, "GATAA":29987, "GATAT":31942, "GATAC":23424, "GATAG":44734, "GATTA":25266, "GATTT":49855, "GATTC":43397, "GATTG":44772, "GATCA":50232, "GATCT":48306, "GATCC":47217, "GATCG":589553, "GATGA":70554, "GATGT":36644, "GATGC":44969, "GATGG":63099, "GACAA":42971, "GACAT":29627, "GACAC":26530, "GACAG":42385, "GACTA":18247, "GACTT":34092, "GACTC":25716, "GACTG":28233, "GACCA":56014, "GACCT":41048, "GACCC":46500, "GACCG":47467, "GACGA":62054, "GACGT":24293, "GACGC":37078, "GACGG":45915, "GAGAA":69308, "GAGAT":173282, "GAGAC":44771, "GAGAG":55359, "GAGTA":19263, "GAGTT":34258, "GAGTC":23203, "GAGTG":21603, "GAGCA":449338, "GAGCT":36271, "GAGCC":45346, "GAGCG":40954, "GAGGA":106150, "GAGGT":42922, "GAGGC":61737, "GAGGG":46128, "GTAAA":19786, "GTAAT":17191, "GTAAC":15160, "GTAAG":16844, "GTATA":11519, "GTATT":22796, "GTATC":21702, "GTATG":18795, "GTACA":16357, "GTACT":16171, "GTACC":20496, "GTACG":14835, "GTAGA":73362, "GTAGT":18523, "GTAGC":23468, "GTAGG":21664, "GTTAA":12894, "GTTAT":20518, "GTTAC":17622, "GTTAG":24642, "GTTTA":21806, "GTTTT":54225, "GTTTC":53332, "GTTTG":51082, "GTTCA":40635, "GTTCT":48306, "GTTCC":59437, "GTTCG":39857, "GTTGA":46988, "GTTGT":46917, "GTTGC":48277, "GTTGG":66953, "GTCAA":35543, "GTCAT":33911, "GTCAC":92531, "GTCAG":37773, "GTCTA":18938, "GTCTT":45998, "GTCTC":46388, "GTCTG":273453, "GTCCA":37620, "GTCCT":43492, "GTCCC":35591, "GTCCG":27772, "GTCGA":45655, "GTCGT":40506, "GTCGC":45128, "GTCGG":43647, "GTGAA":38657, "GTGAT":37942, "GTGAC":25223, "GTGAG":33630, "GTGTA":14515, "GTGTT":33863, "GTGTC":22173, "GTGTG":32045, "GTGCA":27892, "GTGCT":39495, "GTGCC":35451, "GTGCG":30813, "GTGGA":47446, "GTGGT":67597, "GTGGC":51925, "GTGGG":55213, "GCAAA":46121, "GCAAT":34401, "GCAAC":44574, "GCAAG":54114, "GCATA":23470, "GCATT":31499, "GCATC":43987, "GCATG":32646, "GCACA":401692, "GCACT":21409, "GCACC":58271, "GCACG":30513, "GCAGA":106539, "GCAGT":29950, "GCAGC":81225, "GCAGG":87253, "GCTAA":14922, "GCTAT":23272, "GCTAC":23603, "GCTAG":27670, "GCTTA":18506, "GCTTT":49120, "GCTTC":77326, "GCTTG":54334, "GCTCA":40227, "GCTCT":39065, "GCTCC":72744, "GCTCG":48261, "GCTGA":49488, "GCTGT":40032, "GCTGC":86259, "GCTGG":86177, "GCCAA":61409, "GCCAT":48045, "GCCAC":59463, "GCCAG":81291, "GCCTA":25357, "GCCTT":57033, "GCCTC":71279, "GCCTG":63726, "GCCCA":62861, "GCCCT":48865, "GCCCC":65019, "GCCCG":55169, "GCCGA":69452, "GCCGT":50834, "GCCGC":99826, "GCCGG":76066, "GCGAA":43755, "GCGAT":44334, "GCGAC":45683, "GCGAG":52453, "GCGTA":17169, "GCGTT":31279, "GCGTC":33885, "GCGTG":30659, "GCGCA":40598, "GCGCT":39983, "GCGCC":62327, "GCGCG":44579, "GCGGA":57521, "GCGGT":52847, "GCGGC":89303, "GCGGG":61021, "GGAAA":60670, "GGAAT":41569, "GGAAC":50545, "GGAAG":541283, "GGATA":37516, "GGATT":46818, "GGATC":44498, "GGATG":55826, "GGACA":35622, "GGACT":26352, "GGACC":35338, "GGACG":37912, "GGAGA":132619, "GGAGT":29853, "GGAGC":59746, "GGAGG":106660, "GGTAA":21668, "GGTAT":24375, "GGTAC":20599, "GGTAG":45363, "GGTTA":22868, "GGTTT":60323, "GGTTC":51357, "GGTTG":73623, "GGTCA":42057, "GGTCT":48103, "GGTCC":35243, "GGTCG":49440, "GGTGA":57271, "GGTGT":40863, "GGTGC":51224, "GGTGG":97617, "GGCAA":55779, "GGCAT":42470, "GGCAC":51311, "GGCAG":84503, "GGCTA":27411, "GGCTT":56747, "GGCTC":45786, "GGCTG":69920, "GGCCA":61680, "GGCCT":51950, "GGCCC":47262, "GGCCG":67027, "GGCGA":77031, "GGCGT":39436, "GGCGC":59921, "GGCGG":105891, "GGGAA":48403, "GGGAT":44678, "GGGAC":30977, "GGGAG":67700, "GGGTA":23623, "GGGTT":56652, "GGGTC":38328, "GGGTG":53882, "GGGCA":50760, "GGGCT":46441, "GGGCC":43312, "GGGCG":56445, "GGGGA":53029, "GGGGT":50942, "GGGGC":51822, "GGGGG":109555 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 951391, "total_bases": 58034851, "q20_bases": 56358461, "q30_bases": 53670726, "total_cycles": 61, "quality_curves": { "A":[39.3727,39.3458,39.217,39.0517,39.2051,39.2342,39.3355,39.2741,39.297,39.3171,39.292,39.3078,39.3501,39.3192,39.2434,39.2678,39.3269,39.2716,39.3608,39.2519,38.6139,39.2528,39.3008,39.3229,38.8647,39.2466,39.2732,38.7419,39.1719,38.3796,39.2138,39.2867,39.1772,39.2004,39.0721,39.1283,39.1386,39.1478,39.0327,38.9638,38.831,38.6271,38.5549,38.3096,38.2481,38.6038,38.5054,37.451,38.0883,36.9938,36.7587,36.5045,37.4427,37.2201,36.9082,35.7459,35.4371,34.8793,34.5285,35.1256,34.3619], 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"CTTGT":44672, "CTTGC":47398, "CTTGG":54250, "CTCAA":41843, "CTCAT":35567, "CTCAC":31893, "CTCAG":33576, "CTCTA":19225, "CTCTT":56583, "CTCTC":55807, "CTCTG":40331, "CTCCA":67616, "CTCCT":134810, "CTCCC":74371, "CTCCG":59212, "CTCGA":45555, "CTCGT":38762, "CTCGC":47478, "CTCGG":46249, "CTGAA":39898, "CTGAT":39096, "CTGAC":29627, "CTGAG":30670, "CTGTA":15598, "CTGTT":48134, "CTGTC":31684, "CTGTG":30604, "CTGCA":44825, "CTGCT":107029, "CTGCC":72971, "CTGCG":44325, "CTGGA":57480, "CTGGT":60434, "CTGGC":58306, "CTGGG":53224, "CCAAA":60862, "CCAAT":39724, "CCAAC":74765, "CCAAG":52747, "CCATA":26544, "CCATT":40590, "CCATC":65024, "CCATG":41982, "CCACA":59116, "CCACT":33026, "CCACC":126266, "CCACG":43712, "CCAGA":52462, "CCAGT":43157, "CCAGC":86595, "CCAGG":55636, "CCTAA":16626, "CCTAT":22072, "CCTAC":25747, "CCTAG":14067, "CCTTA":18338, "CCTTT":56079, "CCTTC":80571, "CCTTG":57631, "CCTCA":49728, "CCTCT":56737, "CCTCC":137450, "CCTCG":61554, "CCTGA":44520, "CCTGT":45871, "CCTGC":99537, "CCTGG":58595, "CCCAA":66535, "CCCAT":44126, "CCCAC":74096, "CCCAG":59294, "CCCTA":20456, "CCCTT":45488, "CCCTC":64465, "CCCTG":52120, "CCCCA":76015, "CCCCT":55107, "CCCCC":65009, "CCCCG":57034, "CCCGA":46324, "CCCGT":33144, "CCCGC":68507, "CCCGG":52063, "CCGAA":44658, "CCGAT":42592, "CCGAC":46674, "CCGAG":44803, "CCGTA":18795, "CCGTT":36924, "CCGTC":49216, "CCGTG":32602, "CCGCA":49330, "CCGCT":54319, "CCGCC":116528, "CCGCG":50500, "CCGGA":46811, "CCGGT":46695, "CCGGC":74468, "CCGGG":48565, "CGAAA":39515, "CGAAT":30196, "CGAAC":34872, "CGAAG":48752, "CGATA":25211, "CGATT":36011, "CGATC":46931, "CGATG":51997, "CGACA":35843, "CGACT":29521, "CGACC":54157, "CGACG":51450, "CGAGA":41091, "CGAGT":23186, "CGAGC":42051, "CGAGG":51775, "CGTAA":14446, "CGTAT":14700, "CGTAC":13586, "CGTAG":19196, "CGTTA":11974, "CGTTT":33081, "CGTTC":36502, "CGTTG":35664, "CGTCA":33681, "CGTCT":36502, "CGTCC":38867, "CGTCG":51745, "CGTGA":26587, "CGTGT":18502, "CGTGC":26297, "CGTGG":37795, "CGCAA":33812, "CGCAT":26267, "CGCAC":35617, "CGCAG":41221, "CGCTA":14932, "CGCTT":39457, "CGCTC":44585, "CGCTG":51402, "CGCCA":62468, "CGCCT":67459, "CGCCC":64073, "CGCCG":92862, "CGCGA":35317, "CGCGT":22493, "CGCGC":42820, "CGCGG":47949, "CGGAA":40791, "CGGAT":37608, "CGGAC":24862, "CGGAG":48873, "CGGTA":22723, "CGGTT":40704, "CGGTC":43057, "CGGTG":51465, "CGGCA":56402, "CGGCT":53511, "CGGCC":63832, "CGGCG":84985, "CGGGA":42744, "CGGGT":36281, "CGGGC":45973, "CGGGG":45951, "GAAAA":59933, "GAAAT":42610, "GAAAC":43786, "GAAAG":44557, "GAATA":26104, "GAATT":35130, "GAATC":36759, "GAATG":33593, "GAACA":39623, "GAACT":36612, "GAACC":46382, "GAACG":32438, "GAAGA":74618, "GAAGT":34353, "GAAGC":60119, "GAAGG":58170, "GATAA":27091, "GATAT":30039, "GATAC":21939, "GATAG":27014, "GATTA":21144, "GATTT":46745, "GATTC":40451, "GATTG":41668, "GATCA":43403, "GATCT":44657, "GATCC":43435, "GATCG":43607, "GATGA":63129, "GATGT":34139, "GATGC":41816, "GATGG":58218, "GACAA":37997, "GACAT":27102, "GACAC":23858, "GACAG":27678, "GACTA":14506, "GACTT":31755, "GACTC":23651, "GACTG":25828, "GACCA":48026, "GACCT":37851, "GACCC":42200, "GACCG":43170, "GACGA":54293, "GACGT":22193, "GACGC":33329, "GACGG":41694, "GAGAA":60547, "GAGAT":43839, "GAGAC":40941, "GAGAG":40868, "GAGTA":15323, "GAGTT":31641, "GAGTC":21513, "GAGTG":19772, "GAGCA":50686, "GAGCT":33379, "GAGCC":41131, "GAGCG":36628, "GAGGA":90240, "GAGGT":39415, "GAGGC":55744, "GAGGG":40841, "GTAAA":17818, "GTAAT":16170, "GTAAC":13885, "GTAAG":12168, "GTATA":9637, "GTATT":21287, "GTATC":20020, "GTATG":17468, "GTACA":14000, "GTACT":14936, "GTACC":18979, "GTACG":13483, "GTAGA":21807, "GTAGT":17189, "GTAGC":21974, "GTAGG":19767, "GTTAA":11601, "GTTAT":19447, "GTTAC":16549, "GTTAG":11707, "GTTTA":17620, "GTTTT":50951, "GTTTC":49688, "GTTTG":47877, "GTTCA":36080, "GTTCT":45228, "GTTCC":55060, "GTTCG":36674, "GTTGA":41734, "GTTGT":43657, "GTTGC":45131, "GTTGG":62341, "GTCAA":31359, "GTCAT":31594, "GTCAC":25382, "GTCAG":26201, "GTCTA":14334, "GTCTT":42783, "GTCTC":44262, "GTCTG":32679, "GTCCA":32535, "GTCCT":40774, "GTCCC":33211, "GTCCG":25519, "GTCGA":39290, "GTCGT":37354, "GTCGC":41439, "GTCGG":39295, "GTGAA":33896, "GTGAT":34950, "GTGAC":23041, "GTGAG":25179, "GTGTA":10988, "GTGTT":31337, "GTGTC":20343, "GTGTG":28292, "GTGCA":23705, "GTGCT":36254, "GTGCC":32685, "GTGCG":27475, "GTGGA":40599, "GTGGT":61909, "GTGGC":47589, "GTGGG":49553, "GCAAA":39598, "GCAAT":32024, "GCAAC":41088, "GCAAG":40084, "GCATA":18588, "GCATT":29404, "GCATC":40527, "GCATG":30152, "GCACA":31543, "GCACT":19702, "GCACC":52860, "GCACG":27471, "GCAGA":47205, "GCAGT":27974, "GCAGC":75097, "GCAGG":78452, "GCTAA":12835, "GCTAT":21494, "GCTAC":22075, "GCTAG":11814, "GCTTA":14071, "GCTTT":46236, "GCTTC":72171, "GCTTG":50707, "GCTCA":34996, "GCTCT":36203, "GCTCC":68213, "GCTCG":43863, "GCTGA":43217, "GCTGT":37102, "GCTGC":80571, "GCTGG":79275, "GCCAA":52750, "GCCAT":44550, "GCCAC":54143, "GCCAG":55660, "GCCTA":18908, "GCCTT":53541, "GCCTC":66369, "GCCTG":58588, "GCCCA":52680, "GCCCT":45101, "GCCCC":59349, "GCCCG":49451, "GCCGA":58995, "GCCGT":47089, "GCCGC":90711, "GCCGG":68374, "GCGAA":36768, "GCGAT":40488, "GCGAC":41444, "GCGAG":38965, "GCGTA":12799, "GCGTT":28835, "GCGTC":31118, "GCGTG":27088, "GCGCA":33221, "GCGCT":36534, "GCGCC":56931, "GCGCG":39730, "GCGGA":47110, "GCGGT":48506, "GCGGC":80402, "GCGGG":54381, "GGAAA":51400, "GGAAT":38829, "GGAAC":46145, "GGAAG":55998, "GGATA":30403, "GGATT":44041, "GGATC":40598, "GGATG":51317, "GGACA":29136, "GGACT":24395, "GGACC":32168, "GGACG":33952, "GGAGA":73218, "GGAGT":27614, "GGAGC":54032, "GGAGG":93308, "GGTAA":18670, "GGTAT":22767, "GGTAC":18893, "GGTAG":24589, "GGTTA":17315, "GGTTT":56525, "GGTTC":47786, "GGTTG":68168, "GGTCA":35437, "GGTCT":44715, "GGTCC":32794, "GGTCG":44657, "GGTGA":49844, "GGTGT":37381, "GGTGC":46562, "GGTGG":88301, "GGCAA":47392, "GGCAT":39272, "GGCAC":33074, "GGCAG":58942, "GGCTA":20959, "GGCTT":52814, "GGCTC":42374, "GGCTG":64064, "GGCCA":50576, "GGCCT":47930, "GGCCC":42947, "GGCCG":59920, "GGCGA":65943, "GGCGT":35897, "GGCGC":53673, "GGCGG":94333, "GGGAA":40534, "GGGAT":41104, "GGGAC":28157, "GGGAG":49049, "GGGTA":17553, "GGGTT":52587, "GGGTC":35053, "GGGTG":47988, "GGGCA":41723, "GGGCT":42651, "GGGCC":39351, "GGGCG":50307, "GGGGA":43813, "GGGGT":45252, "GGGGC":46392, "GGGGG":38406 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/b2/AM-AL-22A_S4_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/b2/AM-AL-22A_S4_R2_001.fastq.gz -o AM-AL-22A.trim.R1.fastq.gz -O AM-AL-22A.trim.R2.fastq.gz -m --merged_out AM-AL-22A.trim.merged.fastq.gz " }