##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-21C_S3_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051300
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.36891657947303	40.0	40.0	40.0	40.0	40.0
2	39.28111861504804	40.0	40.0	40.0	40.0	40.0
3	39.21097688576049	40.0	40.0	40.0	40.0	40.0
4	39.11789213354894	40.0	40.0	40.0	40.0	40.0
5	39.10035575002378	40.0	40.0	40.0	40.0	40.0
6	39.22558736802055	40.0	40.0	40.0	40.0	40.0
7	39.233238847141635	40.0	40.0	40.0	40.0	40.0
8	39.234338438124226	40.0	40.0	40.0	40.0	40.0
9	39.25896318843337	40.0	40.0	40.0	40.0	40.0
10	39.27800627794159	40.0	40.0	40.0	40.0	40.0
11	39.25597260534576	40.0	40.0	40.0	40.0	40.0
12	39.265473223627886	40.0	40.0	40.0	40.0	40.0
13	39.168899457814135	40.0	40.0	40.0	40.0	40.0
14	39.28225244934843	40.0	40.0	40.0	40.0	40.0
15	39.24023589841149	40.0	40.0	40.0	40.0	40.0
16	39.257117854085415	40.0	40.0	40.0	40.0	40.0
17	39.288983163702085	40.0	40.0	40.0	40.0	40.0
18	39.24061637972034	40.0	40.0	40.0	40.0	40.0
19	39.293959859221914	40.0	40.0	40.0	40.0	40.0
20	38.9294435460858	40.0	40.0	40.0	40.0	40.0
21	39.159239037382285	40.0	40.0	40.0	40.0	40.0
22	39.15241320270142	40.0	40.0	40.0	40.0	40.0
23	39.25685532198231	40.0	40.0	40.0	40.0	40.0
24	39.27961190906497	40.0	40.0	40.0	40.0	40.0
25	39.02838771045372	40.0	40.0	40.0	40.0	40.0
26	39.22472748026253	40.0	40.0	40.0	40.0	40.0
27	39.242480738133736	40.0	40.0	40.0	40.0	40.0
28	38.97680205459907	40.0	40.0	40.0	40.0	40.0
29	39.18462855512223	40.0	40.0	40.0	40.0	40.0
30	38.826359745077525	40.0	40.0	40.0	40.0	40.0
31	39.04083325406639	40.0	40.0	40.0	40.0	40.0
32	39.08082944925331	40.0	40.0	40.0	40.0	40.0
33	39.08507752306668	40.0	40.0	40.0	40.0	40.0
34	38.91981736897175	40.0	40.0	40.0	40.0	40.0
35	39.008846190430894	40.0	40.0	40.0	40.0	40.0
36	38.963211262246745	40.0	40.0	40.0	40.0	40.0
37	39.04975554075906	40.0	40.0	40.0	40.0	40.0
38	38.93160848473319	40.0	40.0	40.0	40.0	40.0
39	38.84159421668411	40.0	40.0	40.0	40.0	40.0
40	38.64900599258061	40.0	40.0	40.0	40.0	40.0
41	38.7404166270332	40.0	40.0	40.0	40.0	40.0
42	38.37152097403215	40.0	40.0	40.0	40.0	40.0
43	38.5674155807096	40.0	40.0	40.0	40.0	40.0
44	38.16582897365167	40.0	40.0	40.0	40.0	40.0
45	38.457901645581664	40.0	40.0	40.0	40.0	40.0
46	38.06871492437934	40.0	40.0	40.0	40.0	40.0
47	37.54918672120232	40.0	40.0	40.0	24.0	40.0
48	37.86332730904594	40.0	40.0	40.0	24.0	40.0
49	37.47430038999334	40.0	40.0	40.0	24.0	40.0
50	37.702185865119375	40.0	40.0	40.0	24.0	40.0
51	37.27751545705317	40.0	40.0	40.0	24.0	40.0
52	37.28281556168553	40.0	40.0	40.0	24.0	40.0
53	36.511330733377726	40.0	40.0	40.0	24.0	40.0
54	35.806875297251025	40.0	40.0	40.0	24.0	40.0
55	35.72178826215162	40.0	40.0	40.0	24.0	40.0
56	35.48768572243888	40.0	40.0	40.0	24.0	40.0
57	35.586199942927806	40.0	40.0	40.0	24.0	40.0
58	35.467113098069056	40.0	40.0	40.0	24.0	40.0
59	34.95756491962332	40.0	40.0	40.0	12.0	40.0
60	34.211496242747074	40.0	24.0	40.0	12.0	40.0
61	34.15809759345572	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.05364292771006518
1101	2	-0.045560353533872444
1101	3	-0.04312121275645353
1101	4	-0.08430548571737972
1101	5	0.09237510524919657
1101	6	0.04778202369290341
1101	7	-0.04635533047611062
1101	8	0.02676571499331004
1101	9	-0.028873065637938566
1101	10	0.06400682390106738
1101	11	0.06744993598500315
1101	12	0.038697455267168834
1101	13	-0.06221124834165437
1101	14	0.12879627527584603
1101	15	0.02079792735723629
1101	16	0.011624342001013588
1101	17	-0.0807337249561968
1101	18	0.0026718777311032227
1101	19	0.11368064779910014
1101	20	0.039817424185201844
1101	21	-0.006258595928812838
1101	22	-0.11601021217658314
1101	23	0.07495292583311652
1101	24	0.004800585600982288
1101	25	-0.015642409932674184
1101	26	0.06487574776954119
1101	27	-0.013696662021317252
1101	28	0.07690802684836484
1101	29	0.04225855534866696
1101	30	0.1023969281055983
1101	31	-0.10681296322916012
1101	32	0.002511859388761195
1101	33	0.09686830847258676
1101	34	0.11788151047534967
1101	35	-5.834407642382189E-4
1101	36	0.05054733943931922
1101	37	0.03542299774090907
1101	38	0.012285239000817683
1101	39	-0.015131710647622754
1101	40	0.1315831607395026
1101	41	0.09185948716780246
1101	42	0.07878270483384142
1101	43	0.15608095233298513
1101	44	7.695019548776827E-4
1101	45	0.21794057102513165
1101	46	-0.11349735273518746
1101	47	-0.08395483445115559
1101	48	0.17512863322811256
1101	49	-0.1008266020356956
1101	50	0.08817432071649733
1101	51	0.12295566415125592
1101	52	0.08065599074411978
1101	53	-0.16426103829822836
1101	54	-0.07341592023173149
1101	55	-0.2510039564047304
1101	56	0.1211701784023731
1101	57	-0.080190101777184
1101	58	0.5176582486940688
1101	59	0.007755904743220299
1101	60	-0.14013796670141687
1101	61	-0.04932637155271635
1102	1	-0.0121772465279264
1102	2	-0.05299212468673886
1102	3	-0.11738378660826498
1102	4	-0.19113652472066178
1102	5	-0.09466442577979706
1102	6	-0.13251173481158673
1102	7	-0.18777702682970698
1102	8	-0.06818745109980995
1102	9	-0.1902316866374818
1102	10	0.033703657978009005
1102	11	-0.07737633289316648
1102	12	-0.11366369938022558
1102	13	0.025221711493585985
1102	14	-0.10049625999570821
1102	15	-0.16322192077426223
1102	16	-0.08802125521074089
1102	17	-0.06438432967150476
1102	18	-0.15860403675322488
1102	19	-0.16035790238791492
1102	20	-0.13970404880708998
1102	21	-0.10232239236910345
1102	22	-0.1496570843576066
1102	23	-0.06495642281369385
1102	24	-0.1331997104955036
1102	25	-0.06504182994876118
1102	26	-0.18626777721436127
1102	27	-0.09935948760885083
1102	28	-0.12247947949112614
1102	29	-0.20584416256286175
1102	30	-0.11852255710183357
1102	31	-0.028285454272420907
1102	32	-0.15801289375825434
1102	33	-0.05734658202989351
1102	34	-0.1162882029358343
1102	35	-0.13274093330319658
1102	36	-0.10220872986730711
1102	37	-0.17557743423056849
1102	38	-0.04264549250250127
1102	39	-0.0903799599816324
1102	40	-0.045006348386586126
1102	41	-0.1854805209274062
1102	42	-0.2200942946453921
1102	43	-0.09574606502123828
1102	44	-0.11804646298042343
1102	45	0.14226446764055822
1102	46	0.022666214257178297
1102	47	-0.07414999497429875
1102	48	0.21945646108828498
1102	49	-0.06041462333960368
1102	50	-0.08624262072854805
1102	51	-0.34763867883051347
1102	52	-0.02496022918503371
1102	53	0.04768389491626124
1102	54	0.08772179202371433
1102	55	0.009960161677973645
1102	56	-0.054470891075219185
1102	57	0.06062758638954335
1102	58	-0.10054896406592917
1102	59	-0.05760873700420177
1102	60	-0.006459891607839552
1102	61	-0.18703271938516508
1103	1	0.05156371373372082
1103	2	-0.014507824300423522
1103	3	0.003455226241605658
1103	4	-0.11726399603068671
1103	5	-0.04298577991435337
1103	6	-0.028766610841564955
1103	7	0.06270113190380755
1103	8	-0.04258576688343396
1103	9	0.020459274913896763
1103	10	-0.10068935987087002
1103	11	0.10416171829739795
1103	12	4.7313332193255064E-4
1103	13	-0.03900001439280487
1103	14	-0.030814514765268086
1103	15	0.042558572398334604
1103	16	0.060574471939034424
1103	17	0.028818343224379817
1103	18	0.04975297965336267
1103	19	-0.07568387995840453
1103	20	0.15901641580129677
1103	21	0.005905120998761504
1103	22	0.1293135722917711
1103	23	0.06322286398111743
1103	24	0.011445616007101478
1103	25	0.11259667784108984
1103	26	0.07496284740958004
1103	27	0.02574385047420691
1103	28	0.08542896868415539
1103	29	0.007284653903035121
1103	30	0.051323083837587546
1103	31	0.063565659858007
1103	32	0.0532426198167002
1103	33	0.10394717509674933
1103	34	0.02317334322324882
1103	35	0.0855592236042142
1103	36	0.08767430029524803
1103	37	-0.017205773498162102
1103	38	0.04279687681587063
1103	39	0.06289975615444376
1103	40	0.012987011266893944
1103	41	0.19534183343014888
1103	42	0.1648087134509737
1103	43	0.18567854523937655
1103	44	0.226773717321322
1103	45	0.1147027670986347
1103	46	0.045175119306151146
1103	47	0.07243705388030719
1103	48	0.34036425927868663
1103	49	0.1830975561476933
1103	50	0.12184254723116794
1103	51	0.2574601077119283
1103	52	0.05801281958432014
1103	53	0.0828361309612049
1103	54	-0.09945080301851306
1103	55	0.3042190920183643
1103	56	0.17879739498618363
1103	57	0.49719826396722056
1103	58	0.3289320670043665
1103	59	0.41028744323241995
1103	60	0.3800024947934446
1103	61	0.6311429287382992
1104	1	-0.08207054667196445
1104	2	-0.16678494134360022
1104	3	-0.034078115110162344
1104	4	0.0018734092515799716
1104	5	-0.13160125596164818
1104	6	-0.09947503939378777
1104	7	-0.03433369875396153
1104	8	-0.014136838405498509
1104	9	0.03842999820012949
1104	10	-0.055125793743563634
1104	11	-0.002261754014242001
1104	12	-0.05271737757016837
1104	13	-0.09786474578709203
1104	14	4.71523963767595E-4
1104	15	-0.17085437925759095
1104	16	-0.11340193971788182
1104	17	-0.10235378658933314
1104	18	-0.12807935491339606
1104	19	-0.09122784809220974
1104	20	-0.09459011025979436
1104	21	-0.11147766248232216
1104	22	-0.0315527014716821
1104	23	-0.09115555417073296
1104	24	-0.11251699922839009
1104	25	-0.17631453688075283
1104	26	0.04276412234042226
1104	27	-0.018807978082264754
1104	28	-0.041186804516826214
1104	29	-0.03652429042310246
1104	30	-0.09585996991185652
1104	31	-0.05296103798620777
1104	32	-0.0692342269581232
1104	33	-0.07272484505309507
1104	34	-0.05380361321543603
1104	35	-0.09208208358902681
1104	36	0.02960905383456236
1104	37	0.03476782227376418
1104	38	-0.09288208399522091
1104	39	-0.08292488071667492
1104	40	0.06779064220201292
1104	41	-0.10091332254015839
1104	42	0.09528889966728826
1104	43	0.13813395449783883
1104	44	-0.06630472417831612
1104	45	0.06845999253216206
1104	46	0.022032506759188664
1104	47	-0.2975263679152036
1104	48	-0.4815557780777837
1104	49	0.1488612057610439
1104	50	-0.016737199622184562
1104	51	-0.0786773187955987
1104	52	8.88559815393819E-4
1104	53	-0.16191946012701663
1104	54	0.17560031449162494
1104	55	-0.04176685685705195
1104	56	0.09620281874857284
1104	57	0.1966980552521278
1104	58	0.17970431284952326
1104	59	0.14725690007792025
1104	60	-0.28664766333476877
1104	61	-0.08941232416071898
1201	1	0.12009220227379558
1201	2	0.03560854184246409
1201	3	-0.05207025842615565
1201	4	0.03175239463620727
1201	5	-0.020563882142141665
1201	6	0.0525136333862406
1201	7	0.10756932401108799
1201	8	0.07575795410582487
1201	9	0.0044263635864751905
1201	10	0.10987482521019132
1201	11	0.04495599391702143
1201	12	0.04117461097076358
1201	13	-0.05115241571553497
1201	14	-0.061986403661862255
1201	15	-0.03796610663666655
1201	16	0.03554250606946141
1201	17	-0.04279212304673763
1201	18	0.04439192599858188
1201	19	0.06879110873997973
1201	20	0.11969607491212741
1201	21	0.028998470441109703
1201	22	-0.003633858395133416
1201	23	0.033363291689276764
1201	24	-0.029862134434992527
1201	25	0.06555427861755447
1201	26	-0.010529627252125806
1201	27	-0.06421574408405206
1201	28	0.03619779723221228
1201	29	0.05034458733683067
1201	30	0.03838853599237524
1201	31	-0.04994171564168681
1201	32	-0.04039830683758083
1201	33	-0.09040494777745067
1201	34	-5.779851604970077E-4
1201	35	-0.02100714770018186
1201	36	-0.09802214055574154
1201	37	0.07152561797801127
1201	38	0.06199333050236078
1201	39	0.03522203766274146
1201	40	0.16980447600157333
1201	41	0.114660579439537
1201	42	0.05550592170131097
1201	43	-0.03345174642517179
1201	44	0.13527349802535582
1201	45	0.15016335326793495
1201	46	0.0963838688603218
1201	47	0.6907435428016271
1201	48	0.331543627360368
1201	49	0.1457049078516377
1201	50	0.010033099680413216
1201	51	-0.06252885193305957
1201	52	-0.018973629581530815
1201	53	0.0926690381739732
1201	54	0.2820149461189132
1201	55	0.2324611539867263
1201	56	0.46878368011245186
1201	57	0.5134492878490917
1201	58	0.33570702892115634
1201	59	0.3509014755891755
1201	60	0.26301769876782544
1201	61	0.25589648248912056
1202	1	-0.19320616171675198
1202	2	-0.12246855803434897
1202	3	-0.14885050497631624
1202	4	-0.11777696912202629
1202	5	-0.06399199077886664
1202	6	-0.05883137555564133
1202	7	-0.053670797039735874
1202	8	0.0166868220104206
1202	9	-0.07515809034202903
1202	10	0.050334310877836685
1202	11	0.007749579783123295
1202	12	-0.04880764806488713
1202	13	0.08351195676306844
1202	14	-0.01277632600326939
1202	15	0.05824333889366784
1202	16	-0.0739908778268088
1202	17	0.010462989677314738
1202	18	-0.1376020661284656
1202	19	-0.02858044226625367
1202	20	0.11499602828511968
1202	21	-0.025537374782842903
1202	22	0.017661889210494053
1202	23	0.05959780450108809
1202	24	-0.013155129088175954
1202	25	-0.056051601767961756
1202	26	0.08458984096196076
1202	27	0.056065101733956624
1202	28	0.054580226226704553
1202	29	0.13748675431937585
1202	30	-0.029401682367385717
1202	31	0.04278727158558837
1202	32	8.110370908980258E-4
1202	33	0.1637344122480684
1202	34	0.12273553855822428
1202	35	0.1774338954926833
1202	36	0.034807174378684635
1202	37	0.03993860990716058
1202	38	0.08611965070855376
1202	39	0.036141397098774064
1202	40	0.15872236870965395
1202	41	0.04144134406759292
1202	42	-0.018048325173609214
1202	43	0.08590937382400199
1202	44	0.138608235477939
1202	45	0.1325388191490262
1202	46	0.048309168983948325
1202	47	0.021452070162460757
1202	48	0.2913451760654553
1202	49	0.18016862125460875
1202	50	0.16628209853606535
1202	51	0.15588473496187305
1202	52	0.2978370462648172
1202	53	-0.07516330376329705
1202	54	0.16948073787466456
1202	55	0.009709625794222632
1202	56	0.5294833708583937
1202	57	0.2885295212733894
1202	58	0.2495552511359307
1202	59	0.26743821088194153
1202	60	0.1985365885653323
1202	61	0.38226684887271034
1203	1	0.01309852515602472
1203	2	0.1290755185371566
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2403	8	-0.011991789061184477
2403	9	0.061817020335659834
2403	10	-0.15147348576190467
2403	11	0.031195960710917348
2403	12	0.040669395530002816
2403	13	-0.13339131247040115
2403	14	-0.006415976356819897
2403	15	0.08028774522217219
2403	16	0.03141100684990761
2403	17	0.010598245111751226
2403	18	-0.05830819610726223
2403	19	-0.034179794303540234
2403	20	0.12054112951628326
2403	21	-0.02660975715590297
2403	22	0.1172667408303596
2403	23	0.030410870807742185
2403	24	-0.0674672460521677
2403	25	-0.0668434247966303
2403	26	0.0142815393902751
2403	27	-0.03611667896886672
2403	28	-0.0501268022346224
2403	29	-0.01919886328099807
2403	30	0.10799777365680541
2403	31	-0.06722444528800509
2403	32	0.07059243699765005
2403	33	-0.0506681177717212
2403	34	-0.05876499229148635
2403	35	-0.03189581257387886
2403	36	-0.06124536418280968
2403	37	0.01611693230687905
2403	38	-0.006360137847075009
2403	39	-0.01772853863724322
2403	40	0.04088367252002456
2403	41	-0.08453460145939573
2403	42	-0.0706645470750118
2403	43	0.011789024170120399
2403	44	-0.021812884546676514
2403	45	0.20962066828774795
2403	46	0.087462273026496
2403	47	-0.06050454832905672
2403	48	-0.2272798967063352
2403	49	-0.28156742126241596
2403	50	0.06138370592985609
2403	51	-0.14136063390969866
2403	52	-0.062240788778730405
2403	53	-0.12361187469580415
2403	54	-0.026076697054321585
2403	55	-0.32269138018664734
2403	56	-0.40976343658849856
2403	57	-0.7431967031482642
2403	58	-0.3145052433434472
2403	59	-0.7012649263845745
2403	60	-0.5539774578880312
2403	61	-0.3967368257833499
2404	1	0.024708857996479594
2404	2	-0.02864310934602088
2404	3	-0.007925511695496823
2404	4	0.050912241468637376
2404	5	0.08160009819835068
2404	6	0.07714269167179566
2404	7	0.016860478251928157
2404	8	0.09557536658500254
2404	9	0.08471646346121275
2404	10	0.06094289678402731
2404	11	0.06699964048549134
2404	12	-0.010120698832913888
2404	13	0.05577502354628905
2404	14	0.04165506070791736
2404	15	0.031097561026548703
2404	16	-0.011994597368691018
2404	17	0.005690926011773456
2404	18	-0.013474221584132806
2404	19	-0.07852435356036125
2404	20	-0.02780360955644312
2404	21	-0.04229624999989312
2404	22	-0.04964417891390838
2404	23	0.021666852014341487
2404	24	0.09691496545215728
2404	25	-0.03275363891393113
2404	26	0.05254959995626507
2404	27	0.0066971879600288275
2404	28	-0.2113430499562412
2404	29	-0.015725933428100802
2404	30	-0.042850845517833136
2404	31	0.07492755426584097
2404	32	-0.04264418634924283
2404	33	0.0045662132985242465
2404	34	-0.04115356638236278
2404	35	-0.04032045122807659
2404	36	0.07584207139687038
2404	37	-0.18177875133072519
2404	38	0.026935147618679878
2404	39	-0.12872197733153712
2404	40	-0.08364222995223258
2404	41	-0.009534815413992703
2404	42	-0.09502508850523128
2404	43	-0.17832190964342232
2404	44	-0.028075594272735316
2404	45	-0.17355725250052245
2404	46	-0.11362495489967017
2404	47	-0.23647961937373907
2404	48	-0.4487021220466971
2404	49	-0.07145892700152245
2404	50	-0.15588657760089575
2404	51	-0.23988846639350214
2404	52	-0.22898430668325176
2404	53	-0.11008580604431017
2404	54	-0.1080197095181461
2404	55	-0.1281057387691007
2404	56	-0.6022706619058269
2404	57	-0.33835472719337645
2404	58	0.014524389321429965
2404	59	0.06858378279206079
2404	60	0.1046110982024473
2404	61	-0.13489062989140876
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	38.0
15	115.0
16	408.0
17	779.0
18	1078.0
19	1128.0
20	1269.0
21	1218.0
22	1403.0
23	1589.0
24	2043.0
25	2242.0
26	2721.0
27	3268.0
28	4405.0
29	5476.0
30	7527.0
31	9325.0
32	11946.0
33	13715.0
34	19338.0
35	33993.0
36	51233.0
37	69043.0
38	144031.0
39	364355.0
40	297608.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.16578726836725	30.714162735770532	18.198646040519858	20.921403955342356
2	29.220774279463523	18.871207076952345	21.026919052601542	30.88109959098259
3	30.38514220488919	19.0758109007895	18.566822029867783	31.972224864453537
4	30.9843051460097	20.258632169694664	19.437268144202417	29.31979454009322
5	27.95034718919433	21.18367735185009	20.58251688385808	30.283458575097498
6	27.056596594692284	20.779130600209264	22.473128507562066	29.691144297536383
7	29.169314182440786	22.931703605060402	21.92542566346428	25.973556549034527
8	27.220869399790736	24.347569675639686	23.21639874441168	25.2151621801579
9	26.99343669742224	23.878721582802246	23.228859507276706	25.89898221249881
10	29.356225625416148	23.738228859507277	21.88861409683249	25.016931418244077
11	27.97136878150861	24.30077047465043	22.179872538761533	25.547988205079424
12	28.372396081042517	22.797774184343194	22.15314372681442	26.676686007799866
13	29.337772281936648	23.69228574146295	21.071150004756017	25.898791971844382
14	29.411966137163514	23.46133358698754	21.263007704746503	25.863692571102444
15	28.560829449253305	23.070103681156663	21.892989631884333	26.4760772377057
16	29.662132597736136	23.488633120897937	20.526871492437934	26.322362788927993
17	29.760772377056977	23.491011129078284	20.847902596784934	25.90031389707981
18	28.46276039189575	22.716160943593646	21.925235422809855	26.89584324170075
19	30.503567012270523	23.56986588033863	20.37876914296585	25.547797964424994
20	29.082088842385616	23.487777037953013	21.61324074954818	25.816893370113192
21	28.736326452962995	23.76181870065633	20.96851517169219	26.533339674688484
22	30.585275373347287	23.729477789403596	20.298202225815658	25.387044611433463
23	29.866641301246077	23.77760867497384	20.74678968895653	25.60896033482355
24	29.463901835822316	23.1168077618187	20.94264244269	26.47664795966898
25	29.877199657566823	24.694663749643297	20.17083610767621	25.25730048511367
26	29.182726148577952	24.938552268619805	20.564348901360223	25.314372681442027
27	29.019785028060497	24.450489869685153	20.76923808617902	25.760487016075334
28	29.831732141158568	25.433177970132213	19.97555407590602	24.759535812803197
29	29.602301911918577	25.409778369637593	20.25825168838581	24.729668030058026
30	29.060496528108054	24.94768382003234	20.474840673451915	25.516978978407685
31	30.34091125273471	25.088366783981737	20.381527632455054	24.1891943308285
32	30.58223152287644	25.314658042423666	20.00770474650433	24.095405688195566
33	29.78269657898116	24.953985322863705	20.64349207406104	24.619826024094095
34	30.48416246551888	25.76714543898031	19.406449158185104	24.342242937315707
35	30.23199847807476	25.870826595643486	19.651954722724245	24.245220203557498
36	30.168077618187006	25.375725292495005	19.83087605821364	24.625321031104345
37	31.167221535242078	26.01017787501189	19.07780842766099	23.744792162085037
38	30.875011890040906	26.04318462855512	19.38209835441834	23.699705126985638
39	31.06534766479597	25.760772377056977	19.143060972129746	24.030818986017312
40	31.862551127175877	26.158565585465617	18.665842290497476	23.31304099686103
41	31.58784362218206	26.36050604014078	19.04261390659184	23.009036431085324
42	31.48340150290117	26.375725292495005	19.317892133548938	22.822981071054883
43	32.675829924854945	26.329972415105107	19.06534766479597	21.928849995243986
44	32.34281365927899	26.46979929610958	19.569580519356986	21.617806525254444
45	32.79824978597926	26.04822600589746	19.328450489869688	21.82507371825359
46	33.484511527654384	26.118569615846315	18.06110160962924	22.33581724687006
47	33.45705317226291	26.27841719775516	18.173879958147058	22.09064967183487
48	33.16655569295158	27.233710643964613	18.32397983449063	21.275753828593167
49	34.288499952439835	26.563968420051363	17.678017692380863	21.46951393512794
50	33.47369922952535	27.571768286882907	18.39446399695615	20.560068486635593
51	33.59954342242937	27.481118615048032	18.285741462950632	20.633596499571958
52	34.04128222201084	27.972129744126317	18.039570056120994	19.947017977741844
53	34.319006558577755	26.338278028526723	17.498989341716644	21.843726071178878
54	33.38580804717968	26.112527347094073	17.43793398649291	23.06373061923333
55	34.47874060686769	26.53257871207077	18.047655283934176	20.941025397127365
56	33.120993056216115	27.538476172358035	19.34728431465804	19.99324645676781
57	33.68914677066489	28.351754970037096	18.24617140682964	19.712926852468375
58	32.675829924854945	29.2336155236374	18.450394749357937	19.64015980214972
59	32.157804622847905	29.671834871111958	17.724626652715685	20.445733853324455
60	31.63323640507451	27.116037108589136	20.54108607210359	20.70964041423276
61	31.903928469513936	28.920955008085226	17.95224959573861	21.222866926662228
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	2.0
4	5.0
5	8.0
6	7.5
7	4.0
8	1.0
9	6.0
10	11.0
11	10.5
12	18.0
13	26.0
14	22.0
15	18.0
16	28.0
17	59.0
18	80.0
19	93.0
20	137.5
21	169.0
22	236.0
23	303.0
24	391.0
25	625.5
26	772.0
27	1002.0
28	1232.0
29	1627.5
30	2321.5
31	2620.0
32	3183.0
33	3746.0
34	4719.5
35	6665.0
36	7637.0
37	8979.5
38	10322.0
39	12111.0
40	16195.5
41	18491.0
42	21404.5
43	27647.0
44	30976.0
45	34972.5
46	38969.0
47	43620.0
48	52918.5
49	57566.0
50	62794.5
51	68023.0
52	72565.0
53	80920.5
54	84734.0
55	86940.5
56	89147.0
57	87986.0
58	84136.0
59	81447.0
60	76642.0
61	66848.5
62	61860.0
63	55295.0
64	48730.0
65	43061.5
66	32142.5
67	26892.0
68	22538.0
69	18184.0
70	15089.0
71	9880.5
72	7767.0
73	6173.0
74	4579.0
75	3609.5
76	2110.0
77	1580.0
78	1244.5
79	909.0
80	697.0
81	375.5
82	266.0
83	208.0
84	113.0
85	76.0
86	64.0
87	52.0
88	49.0
89	44.5
90	43.0
91	38.0
92	33.0
93	38.5
94	48.5
95	53.0
96	59.0
97	65.0
98	81.0
99	270.0
100	443.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001236564253781033
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	4.7560163606962804E-4
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.9024065442785123E-4
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	4.7560163606962804E-4
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	6.658422904974793E-4
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1051300.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.56904905916316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.81806304704152	69.18528745787323
2	11.539140397493588	18.824734183674515
3	2.4547230119060246	6.006882653544578
4	0.6326284119205429	2.064115918726689
5	0.20597248912065727	0.8400490034960422
6	0.09607359131038619	0.4701978889732129
7	0.06323566447722223	0.36106511126219254
8	0.04178622158910183	0.2726769887038808
9	0.029682269955258994	0.2179039080751061
>10	0.11634060288453994	1.3557287526626824
>50	0.0011151910900311789	0.06145196294857964
>100	0.0011151910900311789	0.18819663653002514
>500	0.0	0.0
>1k	1.2391012111457542E-4	0.15170953352930597
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAAAATGTCCCCCCCCATTTCCACCCCCCTTGTCTC	1501	0.14277561114810236	Illumina Single End PCR Primer 1 (96% over 29bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.6954247122610102	0.0	0.0	0.0	0.0
2	0.6966612765147913	0.0	0.0	0.0	0.0
3	0.6977076001141443	0.0	0.0	0.0	0.0
4	0.6984685627318558	0.0	0.0	0.0	0.0
5	0.6993246456767811	0.0	0.0	0.0	0.0
6	0.7027489774564825	0.0	0.0	0.0	0.0
7	0.7057928279273281	0.0	0.0	0.0	0.0
8	0.7179682298107105	0.0	0.0	0.0	0.0
9	0.7203462379910587	0.0	0.0	0.0	1.9024065442785123E-4
10	0.7248168933701132	0.0	0.0	0.0	1.9024065442785123E-4
11	0.7336630838010083	0.0	0.0	0.0	1.9024065442785123E-4
12	0.7483116141919528	0.0	0.0	0.0	1.9024065442785123E-4
13	0.7776086749738419	0.0	0.0	0.0	1.9024065442785123E-4
14	0.8113763911347855	0.0	0.0	0.0	1.9024065442785123E-4
15	0.8557024636164748	0.0	0.0	0.0	1.9024065442785123E-4
16	0.9109673737277656	0.0	0.0	0.0	1.9024065442785123E-4
17	0.9824027394654238	0.0	0.0	0.0	1.9024065442785123E-4
18	1.0966422524493484	0.0	0.0	0.0	1.9024065442785123E-4
19	1.2610101778750118	0.0	0.0	0.0	2.8536098164177684E-4
20	1.4903452867877867	0.0	0.0	0.0	2.8536098164177684E-4
21	1.7957766574717018	0.0	0.0	0.0	2.8536098164177684E-4
22	2.1580899838295444	0.0	0.0	0.0	2.8536098164177684E-4
23	2.597926376866736	0.0	0.0	0.0	2.8536098164177684E-4
24	3.1303148482830783	0.0	0.0	0.0	2.8536098164177684E-4
25	3.72862170645867	0.0	0.0	0.0	2.8536098164177684E-4
26	4.435841339294207	0.0	0.0	0.0	2.8536098164177684E-4
27	5.274231903357747	0.0	0.0	0.0	2.8536098164177684E-4
28	6.175116522400837	0.0	0.0	0.0	2.8536098164177684E-4
29	7.199086844858746	0.0	0.0	0.0	2.8536098164177684E-4
30	8.342528298297346	0.0	0.0	0.0	2.8536098164177684E-4
31	9.570721963283553	0.0	0.0	0.0	4.756016360696281E-4
32	10.907828402929706	0.0	0.0	0.0	4.756016360696281E-4
33	12.341577095025206	0.0	0.0	0.0	4.756016360696281E-4
34	13.855036621325977	0.0	0.0	0.0	4.756016360696281E-4
35	15.449633786740227	0.0	0.0	0.0	4.756016360696281E-4
36	17.11119566251308	0.0	0.0	0.0	4.756016360696281E-4
37	18.78312565395225	9.512032721392562E-5	0.0	0.0	4.756016360696281E-4
38	20.584133929420716	9.512032721392562E-5	0.0	0.0	4.756016360696281E-4
39	22.449728907067442	9.512032721392562E-5	0.0	0.0	4.756016360696281E-4
40	24.32978217445068	9.512032721392562E-5	0.0	0.0	4.756016360696281E-4
41	26.258917530676307	9.512032721392562E-5	0.0	0.0	5.707219632835537E-4
42	28.256634642823172	9.512032721392562E-5	0.0	0.0	5.707219632835537E-4
43	30.23409112527347	9.512032721392562E-5	0.0	0.0	5.707219632835537E-4
44	32.250356701227055	9.512032721392562E-5	0.0	0.0	5.707219632835537E-4
45	34.28098544658994	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
46	36.304860648720634	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
47	38.37895938362028	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
48	40.46152382764197	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
49	42.49719395034719	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
50	44.512508323028634	9.512032721392562E-5	9.512032721392562E-5	0.0	5.707219632835537E-4
>>END_MODULE