##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-21C_S3_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051300
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.49221345001427	40.0	40.0	40.0	40.0	40.0
2	39.22091886236089	40.0	40.0	40.0	40.0	40.0
3	39.13723199847807	40.0	40.0	40.0	40.0	40.0
4	39.00205269666127	40.0	40.0	40.0	40.0	40.0
5	39.06687339484448	40.0	40.0	40.0	40.0	40.0
6	39.17465233520403	40.0	40.0	40.0	40.0	40.0
7	39.21553124702749	40.0	40.0	40.0	40.0	40.0
8	39.2194502045087	40.0	40.0	40.0	40.0	40.0
9	39.220580233996	40.0	40.0	40.0	40.0	40.0
10	39.23656805859412	40.0	40.0	40.0	40.0	40.0
11	39.23291543802911	40.0	40.0	40.0	40.0	40.0
12	39.237568724436414	40.0	40.0	40.0	40.0	40.0
13	39.0606487206316	40.0	40.0	40.0	40.0	40.0
14	39.23166365452297	40.0	40.0	40.0	40.0	40.0
15	39.132335204033105	40.0	40.0	40.0	40.0	40.0
16	39.32350803766765	40.0	40.0	40.0	40.0	40.0
17	39.27001617045563	40.0	40.0	40.0	40.0	40.0
18	39.35802149719395	40.0	40.0	40.0	40.0	40.0
19	39.30389042138305	40.0	40.0	40.0	40.0	40.0
20	39.296676495767144	40.0	40.0	40.0	40.0	40.0
21	39.29115951678874	40.0	40.0	40.0	40.0	40.0
22	39.35633596499572	40.0	40.0	40.0	40.0	40.0
23	39.35787691429658	40.0	40.0	40.0	40.0	40.0
24	39.35009987634358	40.0	40.0	40.0	40.0	40.0
25	39.302665271568536	40.0	40.0	40.0	40.0	40.0
26	39.26861980405213	40.0	40.0	40.0	40.0	40.0
27	39.289424522020354	40.0	40.0	40.0	40.0	40.0
28	39.29693522305717	40.0	40.0	40.0	40.0	40.0
29	39.30501284124417	40.0	40.0	40.0	40.0	40.0
30	39.30106344525825	40.0	40.0	40.0	40.0	40.0
31	39.241590411871016	40.0	40.0	40.0	40.0	40.0
32	39.27057928279273	40.0	40.0	40.0	40.0	40.0
33	39.26951013031485	40.0	40.0	40.0	40.0	40.0
34	39.26037096927613	40.0	40.0	40.0	40.0	40.0
35	39.26634833063826	40.0	40.0	40.0	40.0	40.0
36	39.242282887853136	40.0	40.0	40.0	40.0	40.0
37	39.245505564539144	40.0	40.0	40.0	40.0	40.0
38	39.2372415105108	40.0	40.0	40.0	40.0	40.0
39	37.976311233710646	40.0	40.0	40.0	40.0	40.0
40	39.22190811376391	40.0	40.0	40.0	40.0	40.0
41	39.22582707124513	40.0	40.0	40.0	40.0	40.0
42	39.185016646057264	40.0	40.0	40.0	40.0	40.0
43	39.2023285456102	40.0	40.0	40.0	40.0	40.0
44	39.154091125273474	40.0	40.0	40.0	40.0	40.0
45	39.0195186911443	40.0	40.0	40.0	40.0	40.0
46	39.002693807666695	40.0	40.0	40.0	40.0	40.0
47	39.11737087415581	40.0	40.0	40.0	40.0	40.0
48	39.17330923618377	40.0	40.0	40.0	40.0	40.0
49	39.155738609340816	40.0	40.0	40.0	40.0	40.0
50	39.13276134309902	40.0	40.0	40.0	40.0	40.0
51	39.08603823837154	40.0	40.0	40.0	40.0	40.0
52	39.09251783506135	40.0	40.0	40.0	40.0	40.0
53	39.01118234566727	40.0	40.0	40.0	40.0	40.0
54	38.91377532578712	40.0	40.0	40.0	40.0	40.0
55	38.957024636164746	40.0	40.0	40.0	40.0	40.0
56	38.95792637686674	40.0	40.0	40.0	40.0	40.0
57	38.86741367830305	40.0	40.0	40.0	40.0	40.0
58	38.80624369827832	40.0	40.0	40.0	40.0	40.0
59	38.749685151716925	40.0	40.0	40.0	40.0	40.0
60	38.721948064301344	40.0	40.0	40.0	40.0	40.0
61	38.68443641206126	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.015987152523052828
1101	2	-0.11500070280455787
1101	3	-0.07249238669790259
1101	4	0.05202809113318807
1101	5	-0.03475777845165595
1101	6	0.061330249567610906
1101	7	-0.10091482392891749
1101	8	-0.01887889939934695
1101	9	-0.1562899116746479
1101	10	0.02907877179979579
1101	11	0.021858714217188435
1101	12	-0.0127610559415956
1101	13	0.045809760348774375
1101	14	-0.0747000374303255
1101	15	-0.046450604980051935
1101	16	0.009896274020405826
1101	17	-0.07282187040063093
1101	18	-0.026926073200094436
1101	19	-0.04076366085431715
1101	20	0.03546953560566379
1101	21	-0.04699209211617017
1101	22	-0.06966076719907477
1101	23	0.03266294685422366
1101	24	-0.012574585880365419
1101	25	-0.0182752453669508
1101	26	-0.06187442667967247
1101	27	-0.021383824537821283
1101	28	-0.010530209768177201
1101	29	-0.03262156332876032
1101	30	0.04275973127965926
1101	31	-0.011756248906593214
1101	32	0.031408153999343824
1101	33	-0.01992723990077394
1101	34	0.03966394165660603
1101	35	-0.08697300486847581
1101	36	-0.07896811438893536
1101	37	-0.15591558023832874
1101	38	-0.08382748637573201
1101	39	1.122488070751217
1101	40	-0.0970986962891871
1101	41	-0.14388999936269187
1101	42	-0.04697953305507241
1101	43	-0.12000149262903648
1101	44	-0.07650781356273484
1101	45	-0.16437656895070774
1101	46	-0.16176288580052045
1101	47	-0.06913759611180126
1101	48	0.024664517018344156
1101	49	-0.04294543883452917
1101	50	-0.10988593999339713
1101	51	-0.145352126133119
1101	52	-0.01298435692183375
1101	53	0.0355788202770384
1101	54	-0.08879063164776113
1101	55	-0.1584055922011558
1101	56	-0.056896314449460306
1101	57	-0.011419458886834377
1101	58	-0.1793854494474374
1101	59	-0.15205852249658847
1101	60	-0.12653499469985263
1101	61	-0.10773249144814656
1102	1	0.03301592068635273
1102	2	-0.1387238672704001
1102	3	-0.02606753693464725
1102	4	-0.03767052596732867
1102	5	-0.22216608744892596
1102	6	-0.1483114325110506
1102	7	-0.28617526978864305
1102	8	-0.2214228587177658
1102	9	-0.2015260790547302
1102	10	-0.10189469283520225
1102	11	-0.22973378899216357
1102	12	-0.2789270915981277
1102	13	-0.14930815640658324
1102	14	-0.25242559752081206
1102	15	-0.07948308590518849
1102	16	0.0011225807072605676
1102	17	-0.19853827978499083
1102	18	-0.0672403078327477
1102	19	-0.044327559782658454
1102	20	-0.1224795003532293
1102	21	-0.17714099785705884
1102	22	-0.06593789990382959
1102	23	-0.059870606122792935
1102	24	-0.08402764447291844
1102	25	-0.010908994959905272
1102	26	-0.12379153147903565
1102	27	-0.25593842757609053
1102	28	-0.030647584992777865
1102	29	-0.13242075835448475
1102	30	-0.1887161127788204
1102	31	-0.15399068893783152
1102	32	-0.18099076995666508
1102	33	-0.09708883822745662
1102	34	-0.22222068919933946
1102	35	-0.09724723067193963
1102	36	-0.12329451010165826
1102	37	-0.26933628769410234
1102	38	-0.04287236575937925
1102	39	1.0872866025136503
1102	40	-0.09601993122277008
1102	41	-0.19255687595830295
1102	42	-0.15652055306986767
1102	43	-0.15309304963647463
1102	44	-0.14460606675459076
1102	45	-0.21168039921116133
1102	46	-0.039753590089461
1102	47	-0.05922928398170768
1102	48	-0.21421875168635296
1102	49	-0.2873363885935447
1102	50	-0.145949919032482
1102	51	-0.11837297444059658
1102	52	-0.03776373013176482
1102	53	-0.16499951666680346
1102	54	-0.182019492211559
1102	55	-0.3385203322744488
1102	56	-0.3142850764242269
1102	57	-0.2221767838287434
1102	58	-0.16393222042933786
1102	59	-0.18624459819238837
1102	60	-0.22824646460766473
1102	61	-0.11109875402844693
1103	1	-0.11003197604635773
1103	2	0.04530911603214349
1103	3	0.09405398627281158
1103	4	-0.007050438928260405
1103	5	-0.011738193237121664
1103	6	0.08836685021901758
1103	7	-0.012528891199615089
1103	8	0.03223515849722958
1103	9	0.08634825451868267
1103	10	-0.018237815334764207
1103	11	0.004182469612175055
1103	12	0.08485662167969821
1103	13	-0.08902146422958168
1103	14	0.06671752739354986
1103	15	-0.0066459961178182425
1103	16	-0.0443214789742683
1103	17	0.05342791075661779
1103	18	0.022910930275941155
1103	19	-0.035909404916488086
1103	20	0.001835001590599461
1103	21	0.12268442275340874
1103	22	0.07772906489362441
1103	23	-0.021832678690962837
1103	24	0.04485083799075085
1103	25	-0.03752453119011534
1103	26	0.10588089114249044
1103	27	0.012493802673326115
1103	28	-0.04277429417586376
1103	29	-0.0618930997873548
1103	30	-0.01679520348051966
1103	31	-0.04665189680475379
1103	32	0.0676243305110944
1103	33	0.020912779325684028
1103	34	0.06697044458979917
1103	35	-0.016727403754877912
1103	36	0.09631439767311889
1103	37	0.016950128975658174
1103	38	0.01212078223282731
1103	39	1.3563038740370104
1103	40	0.022770522469606647
1103	41	-0.048107294972624004
1103	42	0.15204133754643578
1103	43	0.03893037543077327
1103	44	0.2038286368400506
1103	45	0.016501796325442797
1103	46	0.1451007926252217
1103	47	0.0757560927305434
1103	48	0.06668630972560408
1103	49	0.013357264001335523
1103	50	0.14636399604066952
1103	51	0.14586627707245725
1103	52	0.026455068718860275
1103	53	0.19745715177010936
1103	54	0.0806398917462019
1103	55	0.04931410214265952
1103	56	0.016511309637884608
1103	57	0.0662558818172414
1103	58	0.03317473400641546
1103	59	-0.0047252068548431225
1103	60	0.1226279232988503
1103	61	0.27225731566500855
1104	1	0.02840626904883692
1104	2	0.028909062261476492
1104	3	-0.05462191102520819
1104	4	-0.1473777324948884
1104	5	-0.11259357136519554
1104	6	-0.05056665102973312
1104	7	-0.05737079836031711
1104	8	0.005979507270765794
1104	9	0.0052818972691923705
1104	10	0.02873369328623454
1104	11	-0.15083978153928967
1104	12	0.012428946765837168
1104	13	-0.20139508547182317
1104	14	0.04685068054797625
1104	15	-0.16222717921402108
1104	16	-0.03490453696876017
1104	17	-0.0863050754257344
1104	18	-0.07983349632356607
1104	19	-0.18206657091927525
1104	20	-0.08167933607504807
1104	21	-0.017798522747185075
1104	22	-0.005134364684487025
1104	23	0.015983666177127986
1104	24	-0.039413458993138306
1104	25	-0.09073626734593887
1104	26	-0.03480015681952864
1104	27	-0.03317588977260044
1104	28	-0.04577413144883735
1104	29	-0.016096055759078354
1104	30	-0.044253619651343
1104	31	-0.12960730252567032
1104	32	0.034505844013892784
1104	33	-0.029702399567909765
1104	34	-0.0984946363138306
1104	35	0.039045514223623456
1104	36	-0.053153909470601945
1104	37	-0.2185300218652202
1104	38	-0.06932762976369133
1104	39	0.9743269529737688
1104	40	-0.07700944229645756
1104	41	-0.05648419905565305
1104	42	-0.16146288274349985
1104	43	-0.05112134346183694
1104	44	-0.1230474995959483
1104	45	-0.055119318566909214
1104	46	-0.05935509773922121
1104	47	-0.04759806494487151
1104	48	-0.07238161819340405
1104	49	-0.029340891523247592
1104	50	-0.04994139305378553
1104	51	-0.07022813290058849
1104	52	-0.20633654871698326
1104	53	0.008092423913367952
1104	54	-0.10868238558309429
1104	55	0.0313999795596871
1104	56	-0.026137320271338638
1104	57	-0.03283072433383438
1104	58	-0.07626272575402027
1104	59	-0.1196317095681394
1104	60	-0.04442197729616737
1104	61	0.11560071920679604
1201	1	0.1226227505054922
1201	2	0.12052007802684983
1201	3	-0.0112248291174879
1201	4	0.005460628281170443
1201	5	-0.0264198262847799
1201	6	0.021528642943998477
1201	7	-0.016688323101980984
1201	8	-0.04677219997949322
1201	9	-0.002995235795609119
1201	10	0.030225774706849506
1201	11	0.014445775888994206
1201	12	0.0838825798046301
1201	13	0.08084269971386959
1201	14	-0.021429074829782735
1201	15	-0.005572547352329593
1201	16	0.09161034319227213
1201	17	0.036854269640620885
1201	18	0.06183000232535818
1201	19	0.03096339449989216
1201	20	0.06689007501059763
1201	21	0.07484676840645932
1201	22	0.11700388980732157
1201	23	-0.0374097020080697
1201	24	0.05111347208764272
1201	25	0.06786898445836442
1201	26	0.045438224612738054
1201	27	0.15443009232388505
1201	28	0.023041806354001437
1201	29	0.17793239560397467
1201	30	0.08486745869062418
1201	31	0.06576746481730567
1201	32	0.05308397905129425
1201	33	0.13665772017949251
1201	34	0.1438341826857794
1201	35	0.07057260132469878
1201	36	0.18251291614632237
1201	37	0.09810547736676511
1201	38	0.20153678859923474
1201	39	1.321476506118458
1201	40	0.11156782107825336
1201	41	0.17107543109566592
1201	42	0.11861112801091167
1201	43	0.014203548660752574
1201	44	0.14260964983267144
1201	45	0.09916863922996555
1201	46	0.21415653822514713
1201	47	0.05893156755774953
1201	48	0.13667385837535306
1201	49	0.10820381781387312
1201	50	0.19039578658270528
1201	51	0.06360871381935596
1201	52	0.09483462898720063
1201	53	0.1744078617920195
1201	54	0.08966042982877553
1201	55	0.08332910093102441
1201	56	0.10630413366201452
1201	57	0.12291635284933022
1201	58	0.03995115243441205
1201	59	0.21073504067560123
1201	60	0.055437604437592825
1201	61	0.2823593133247613
1202	1	0.08973121130338058
1202	2	0.1123388408311996
1202	3	0.05454872983419534
1202	4	0.0022456162509669753
1202	5	0.025989153561255307
1202	6	0.05982723119088007
1202	7	-0.003283835522218226
1202	8	0.1236768930088985
1202	9	0.08627343043205826
1202	10	0.08523905708914725
1202	11	0.14922877539160595
1202	12	0.040798635990640264
1202	13	-0.07501856451703048
1202	14	0.0030146825054586657
1202	15	0.0314163839042223
1202	16	-0.02001109600616502
1202	17	0.037526489443315825
1202	18	0.056208765598363186
1202	19	0.05680285003113994
1202	20	-0.011699596127101586
1202	21	-0.01268240093526174
1202	22	-0.0220657924022305
1202	23	0.05777493784266596
1202	24	0.08070981617850492
1202	25	0.09426972155689839
1202	26	0.02538915915752682
1202	27	0.07840042165484107
1202	28	-0.017407812034946346
1202	29	0.12606016642827456
1202	30	0.06610132253206302
1202	31	0.024836747942117654
1202	32	0.07386860528188777
1202	33	0.05495848325514885
1202	34	-0.03461088430645276
1202	35	0.06983996961163541
1202	36	-0.06147284934306185
1202	37	-0.010225160313900972
1202	38	-0.07886344579897298
1202	39	1.238942852565387
1202	40	-0.2150207970615412
1202	41	-0.11129679451023122
1202	42	-0.03146194294068039
1202	43	-0.09001944524734995
1202	44	0.02775415969894368
1202	45	-0.11476384844646503
1202	46	0.0070429076322611195
1202	47	-0.03630433088360974
1202	48	-0.05742265312972705
1202	49	0.08961305926179364
1202	50	-0.06679419185024216
1202	51	-0.05035560910317116
1202	52	-0.0016516129324770645
1202	53	0.06467566779070211
1202	54	0.0018021071087162
1202	55	-0.13837858267678627
1202	56	0.04148654742538582
1202	57	-0.016574382068881732
1202	58	0.028126527844094085
1202	59	-0.03356486674772441
1202	60	0.012268351683495382
1202	61	0.11352266914413889
1203	1	0.06701188504350597
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2403	6	-0.11800059440057709
2403	7	0.03623899147986265
2403	8	-0.02231722839604089
2403	9	-0.038740863819292315
2403	10	-0.11888220200889776
2403	11	0.004369090363979922
2403	12	-0.019604806074376313
2403	13	-0.04466226584516164
2403	14	0.013295581846342941
2403	15	0.0653829622492168
2403	16	-0.002968168105020652
2403	17	-0.031602786047386644
2403	18	0.0859891139312694
2403	19	0.00426911908150629
2403	20	0.02615371805371325
2403	21	0.02414349215702316
2403	22	-0.01476720107457652
2403	23	0.001222559850546645
2403	24	0.026506572407619444
2403	25	-0.08477986191488895
2403	26	-0.10920894873413545
2403	27	0.08907556829225882
2403	28	-0.10704426382042698
2403	29	0.025725158410956794
2403	30	-0.0482749831682554
2403	31	-0.025768849905020375
2403	32	-0.030807953548602995
2403	33	-0.03489199910790575
2403	34	-0.08607446236820948
2403	35	-0.06035416188173315
2403	36	-0.05068362738044385
2403	37	-0.024264971673439106
2403	38	0.029271044550469583
2403	39	1.1898551625470617
2403	40	0.015617958803979093
2403	41	-0.16931728849540661
2403	42	-0.024102783932214322
2403	43	0.07431219178837978
2403	44	-0.001497057483845765
2403	45	-0.0012016764188302886
2403	46	0.07012470456387376
2403	47	-0.021184333952867007
2403	48	0.10937405135674538
2403	49	0.09501733134555934
2403	50	0.10636137595922435
2403	51	-0.09814127977458043
2403	52	0.008627428504084378
2403	53	0.039755225576030284
2403	54	-0.06697416656133015
2403	55	0.05473645123414883
2403	56	0.0013636173282023378
2403	57	-0.0350932291612196
2403	58	-0.058281629396297774
2403	59	-0.049843452811934696
2403	60	-0.025892631452585135
2403	61	0.0931808463337731
2404	1	-0.1284157232008667
2404	2	-0.014617075748063257
2404	3	0.06483483291823688
2404	4	0.05589561685253841
2404	5	0.044715136098872676
2404	6	-0.10576220327144625
2404	7	0.07654032607692329
2404	8	0.08484063915656037
2404	9	0.04811511050314721
2404	10	0.11175464819570635
2404	11	0.01638214733129928
2404	12	-0.0027088819612046677
2404	13	-0.0167772573664422
2404	14	-0.08176646827885037
2404	15	1.1053973675245743E-4
2404	16	-0.029571518003926656
2404	17	0.006785653369647093
2404	18	-0.07988429059048485
2404	19	-0.0376569538208642
2404	20	-0.14912654131004643
2404	21	0.00689658845624308
2404	22	-0.012999136699725966
2404	23	-0.017466632959411754
2404	24	-0.017932032826585953
2404	25	-0.023408466544374562
2404	26	-0.053876057479591566
2404	27	0.06190756634513406
2404	28	-0.062017683135607626
2404	29	0.07568501488550794
2404	30	0.008622833149978248
2404	31	0.0059682818074051625
2404	32	-0.023220324093138345
2404	33	-0.2147443977082375
2404	34	0.0326284893682427
2404	35	0.06071912466874352
2404	36	-0.0715528059461974
2404	37	-0.023111967990693927
2404	38	0.016064356038171468
2404	39	1.2356630772119956
2404	40	-0.026465735156492087
2404	41	0.07981577217356062
2404	42	0.018794219640682286
2404	43	0.12576248891253528
2404	44	0.06591058489917856
2404	45	0.046503709885840294
2404	46	0.06912179826103682
2404	47	0.06765450575677079
2404	48	0.0022131743328657194
2404	49	0.1076804341085662
2404	50	0.0505789449327807
2404	51	0.0011351586892089927
2404	52	-0.004257622765550195
2404	53	0.007518145447825475
2404	54	0.07286655905228656
2404	55	-0.06236320467983347
2404	56	0.04650606265715851
2404	57	0.03389664274533999
2404	58	0.047400844863084046
2404	59	-0.04553689941550232
2404	60	-0.09134261277034739
2404	61	0.02917541122864975
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	12.0
17	52.0
18	123.0
19	169.0
20	222.0
21	251.0
22	398.0
23	556.0
24	858.0
25	1082.0
26	1472.0
27	1757.0
28	2497.0
29	3056.0
30	3848.0
31	4737.0
32	6069.0
33	7583.0
34	10011.0
35	12607.0
36	18731.0
37	27818.0
38	72052.0
39	505518.0
40	369820.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.019309426424424	11.042043184628556	32.31675068962237	30.621896699324648
2	27.794376232197365	16.587469837330918	28.581174688477667	27.036979241994047
3	27.719299914391705	19.542376105773805	20.803861885284885	31.934462094549605
4	26.416842401638352	19.601654714786186	20.478950327166675	33.50255255640879
5	27.38694949110625	19.818605536003044	19.931988966042045	32.86245600684866
6	29.11728336345477	22.108246932369447	23.005421858651193	25.76904784552459
7	26.91058689241891	21.621135736706933	22.580138875677733	28.888138495196426
8	25.18805288690193	23.494435460857986	23.07971083420527	28.237800818034813
9	25.698373442404645	23.66755445638733	23.323123751545705	27.310948349662322
10	25.8565585465614	21.669076381622755	23.364596214210977	29.109768857604866
11	25.259583372966805	22.26405402834586	23.208503757252924	29.267858841434414
12	26.851707409873487	22.392846951393512	22.84133929420717	27.914106344525823
13	25.639493959859223	20.93141824407876	23.045752877389898	30.383334918672123
14	25.973746789688956	21.713212213450014	22.959859221915725	29.35318177494531
15	26.567678112812708	21.66298868068106	22.56120993056216	29.20812327594407
16	26.083325406639403	21.65766194235708	22.6680300580234	29.59098259298012
17	26.26186626081994	21.08218396271283	22.652335204033104	30.003614572434127
18	27.63901835822315	21.927232949681347	22.413963664035006	28.019785028060497
19	26.07533529915343	20.951108151812043	23.333967468848094	29.639589080186436
20	26.160848473318747	21.42233425282983	22.52354228098545	29.893274992865976
21	26.677542090744794	22.134024541044422	22.910206411110053	28.27822695710073
22	26.878531342147816	21.176733567963474	22.720441358318272	29.224293731570437
23	26.23913250261581	22.262056501474365	22.61381147151146	28.884999524398363
24	26.721868163226482	23.104632359935316	22.337772281936648	27.83572719490155
25	25.990392846951394	21.541044421192808	22.832873585085135	29.635689146770666
26	26.39417863597451	21.77760867497384	23.280890326262725	28.54732236278893
27	26.824502996290306	22.67430799961952	22.303053362503565	28.198135641586607
28	26.183772472177303	22.26414914867307	22.55978312565395	28.99229525349567
29	26.510701036811568	23.377913060020926	22.372110720060878	27.73927518310663
30	27.64092076476743	23.43764862551127	22.099305621611336	26.82212498810996
31	26.5389517739941	22.820032340911254	22.94578141348806	27.695234471606582
32	26.80566917150195	23.253305431370684	22.060211167126415	27.88081423000095
33	27.132217254827356	24.69837344240464	21.811471511462	26.357937791306004
34	26.81289831637021	23.714448777703794	21.855036621325976	27.61761628460002
35	27.897840768572244	24.024350803766765	21.43422429373157	26.643584133929423
36	27.70341481974698	24.81242271473414	21.746218966993247	25.737943498525635
37	27.205269666127652	24.432321887187292	21.17150194996671	27.19090649671835
38	27.231522876438696	24.9959098259298	20.819937220584038	26.952630077047463
39	28.07312233551404	25.464038034616216	21.012553780868746	25.450285849001002
40	27.4303243603158	26.11024445924094	20.35812803196043	26.10130314848283
41	27.62513079044992	26.325501759726055	20.10853229335109	25.940835156472936
42	28.52563492818415	26.506040140778087	19.72595833729668	25.24236659374108
43	27.43612670027585	26.836488157519263	19.866451060591647	25.86093408161324
44	27.714448777703794	26.36840102729953	20.221249881099588	25.69590031389708
45	27.786645106059165	26.94815942166841	19.262246742128795	26.002948730143633
46	27.343003899933414	26.780462284790257	19.848948920384284	26.027584894892037
47	28.24598116617521	27.006087700941688	18.91515266812518	25.832778464757915
48	28.31104346998954	27.57623894226196	18.718348711119567	25.39436887662894
49	27.91562826976125	27.371539998097592	18.753352991534292	25.95947874060687
50	28.042994387900695	27.440121754018833	18.52677637211072	25.990107485969755
51	27.916769713687817	27.824883477599162	18.51517169219062	25.743175116522398
52	27.49966707885475	27.71872919242842	18.451345952630078	26.330257776086754
53	27.607818890896986	27.829829734614286	18.31836773518501	26.24398363930372
54	27.383620279653766	28.21031104346999	18.174355559783127	26.231713117093125
55	27.09597641015885	28.009131551412537	18.17036050604014	26.724531532388475
56	27.00323409112527	27.967278607438413	18.169980024731284	26.85950727670503
57	26.769999048796727	28.152477884523925	18.080566917150197	26.996956149529154
58	26.27375630172168	28.04651384000761	18.212498810995907	27.467231047274804
59	26.23932274327024	27.99610006658423	18.211167126414914	27.55341006373062
60	26.060972129744126	28.152287643869496	18.155711975649197	27.631028250737184
61	25.936364501093884	27.978312565395225	18.124892989631885	27.960429943879006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	2.5
3	2.0
4	2.0
5	2.0
6	4.0
7	6.5
8	7.0
9	7.5
10	8.0
11	9.5
12	11.5
13	12.0
14	17.0
15	22.0
16	30.0
17	49.5
18	61.0
19	88.5
20	131.0
21	146.0
22	205.5
23	265.0
24	389.0
25	668.0
26	823.0
27	1028.5
28	1234.0
29	1542.5
30	2344.5
31	2838.0
32	3245.5
33	3653.0
34	4709.5
35	6799.5
36	7833.0
37	9216.5
38	10600.0
39	12546.0
40	16875.5
41	19259.0
42	21966.0
43	27899.5
44	31126.0
45	35448.5
46	39771.0
47	44695.5
48	54541.0
49	59462.0
50	65233.0
51	71004.0
52	75406.5
53	83221.0
54	86633.0
55	88002.5
56	89372.0
57	88896.5
58	85162.5
59	81904.0
60	76747.5
61	67950.0
62	64309.0
63	54471.5
64	44634.0
65	38708.0
66	28107.0
67	23432.0
68	19665.0
69	15898.0
70	13265.0
71	8714.5
72	6797.0
73	5515.0
74	4233.0
75	3350.0
76	1941.0
77	1415.0
78	1104.0
79	793.0
80	638.5
81	344.0
82	204.0
83	166.0
84	92.0
85	56.0
86	44.5
87	33.0
88	28.0
89	14.5
90	6.0
91	4.0
92	2.0
93	1.5
94	3.5
95	6.0
96	5.5
97	5.0
98	7.0
99	5.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.03157994863502331
3	0.0
4	9.512032721392562E-5
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.1656044896794446
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1051300.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.1265743095482
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.06254388695905	61.74949740509012
2	15.064866794774447	23.238031366212333
3	3.3399779775727056	7.728031790385474
4	0.849000881283059	2.6192211823659903
5	0.26682453096208864	1.0289631007428937
6	0.1230398148833236	0.5693783655379028
7	0.0713146763684968	0.38501796804073746
8	0.051704035233108084	0.31902040924078473
9	0.04093540948514996	0.28414871113933826
>10	0.12872798080009576	1.3793028766053688
>50	5.320058392388469E-4	0.03364606607239816
>100	3.9900437942913525E-4	0.041749844181298935
>500	0.0	0.0
>1k	0.0	0.0
>5k	1.3300145980971173E-4	0.6239909143853598
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTAAGATCTGGGGGGGGGGGGGGG	6083	0.5786169504423095	TruSeq Adapter, Index 18 (97% over 37bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.7028440977836964	0.0	0.0	0.0	0.0
2	0.7047465043279749	0.0	0.0	0.0	9.512032721392562E-5
3	0.7062684295633977	0.0	0.0	0.0	9.512032721392562E-5
4	0.7072196328355369	0.0	0.0	0.0	9.512032721392562E-5
5	0.7086464377437458	0.0	0.0	0.0	9.512032721392562E-5
6	0.7124512508323029	0.0	0.0	0.0	9.512032721392562E-5
7	0.7142585370493675	0.0	0.0	0.0	9.512032721392562E-5
8	0.7497384191001617	0.0	0.0	0.0	1.9024065442785123E-4
9	0.7535432321887188	0.0	0.0	0.0	1.9024065442785123E-4
10	0.7584894892038428	0.0	0.0	0.0	1.9024065442785123E-4
11	0.7656235137448872	0.0	0.0	0.0	1.9024065442785123E-4
12	0.7797964424997622	0.0	0.0	0.0	1.9024065442785123E-4
13	0.8088081423000095	0.0	0.0	0.0	1.9024065442785123E-4
14	0.8411490535527443	0.0	0.0	0.0	1.9024065442785123E-4
15	0.8841434414534386	0.0	0.0	0.0	1.9024065442785123E-4
16	0.9376010653476647	0.0	0.0	0.0	1.9024065442785123E-4
17	1.007799866831542	0.0	0.0	0.0	1.9024065442785123E-4
18	1.116332160182631	0.0	0.0	0.0	1.9024065442785123E-4
19	1.2833634547702844	0.0	0.0	0.0	1.9024065442785123E-4
20	1.507847426995149	0.0	0.0	0.0	1.9024065442785123E-4
21	1.8135641586607059	0.0	0.0	0.0	1.9024065442785123E-4
22	2.1794920574526775	0.0	0.0	0.0	1.9024065442785123E-4
23	2.6224674212879293	0.0	0.0	0.0	1.9024065442785123E-4
24	3.1596119090649673	0.0	0.0	0.0	1.9024065442785123E-4
25	3.763055264910111	0.0	0.0	0.0	1.9024065442785123E-4
26	4.471796822981071	0.0	0.0	0.0	1.9024065442785123E-4
27	5.315894606677447	0.0	0.0	0.0	1.9024065442785123E-4
28	6.196138114715114	0.0	0.0	0.0	2.8536098164177684E-4
29	7.1890992105012845	0.0	0.0	0.0	2.8536098164177684E-4
30	8.304194806430134	0.0	0.0	0.0	2.8536098164177684E-4
31	9.493389137258632	0.0	0.0	0.0	2.8536098164177684E-4
32	10.78360125558832	0.0	0.0	0.0	3.8048130885570247E-4
33	12.181584704651383	0.0	0.0	0.0	3.8048130885570247E-4
34	13.654332730904594	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
35	15.20403310187387	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
36	16.822220108437172	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
37	18.45163131361172	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
38	20.226101017787503	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
39	22.045562636735472	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
40	23.931798725387615	9.512032721392562E-5	0.0	0.0	3.8048130885570247E-4
41	25.872348520878912	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
42	27.88176543327309	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
43	29.874821649386472	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
44	31.91591363074289	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
45	33.97717112146866	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
46	36.03405307714259	1.9024065442785123E-4	0.0	0.0	3.8048130885570247E-4
47	38.13193189384572	1.9024065442785123E-4	0.0	0.0	5.707219632835537E-4
48	40.238086179016456	3.8048130885570247E-4	0.0	0.0	5.707219632835537E-4
49	42.29411205174546	3.8048130885570247E-4	0.0	0.0	5.707219632835537E-4
50	44.322457909255206	3.8048130885570247E-4	0.0	0.0	5.707219632835537E-4
>>END_MODULE