##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-21C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 636320 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.590602212723155 40.0 40.0 40.0 40.0 40.0 2 39.36842783505155 40.0 40.0 40.0 40.0 40.0 3 39.29943424691979 40.0 40.0 40.0 40.0 40.0 4 39.16901244656776 40.0 40.0 40.0 40.0 40.0 5 39.23433492582348 40.0 40.0 40.0 40.0 40.0 6 39.34363842092029 40.0 40.0 40.0 40.0 40.0 7 39.391538848378175 40.0 40.0 40.0 40.0 40.0 8 39.39473849635404 40.0 40.0 40.0 40.0 40.0 9 39.39246291174252 40.0 40.0 40.0 40.0 40.0 10-11 39.4066067387478 40.0 40.0 40.0 40.0 40.0 12-13 39.32195436258486 40.0 40.0 40.0 40.0 40.0 14-15 39.35609441790294 40.0 40.0 40.0 40.0 40.0 16-17 39.4640935378426 40.0 40.0 40.0 40.0 40.0 18-19 39.50335365853658 40.0 40.0 40.0 40.0 40.0 20-21 39.480638672366105 40.0 40.0 40.0 40.0 40.0 22-23 39.52835365853659 40.0 40.0 40.0 40.0 40.0 24-25 39.49912936887101 40.0 40.0 40.0 40.0 40.0 26-27 39.478881694744786 40.0 40.0 40.0 40.0 40.0 28-29 39.49801986421926 40.0 40.0 40.0 40.0 40.0 30-31 39.46126713775936 40.0 40.0 40.0 40.0 40.0 32-33 39.462269484720814 40.0 40.0 40.0 40.0 40.0 34-35 39.45821464943132 40.0 40.0 40.0 40.0 40.0 36-37 39.44733108176108 40.0 40.0 40.0 40.0 40.0 38-39 39.38434661635408 40.0 40.0 40.0 40.0 40.0 40-41 39.42555402925877 40.0 40.0 40.0 40.0 40.0 42-43 39.40257631450842 40.0 40.0 40.0 40.0 40.0 44-45 39.296743473833374 40.0 40.0 40.0 40.0 40.0 46-47 39.282742425770735 40.0 40.0 40.0 40.0 40.0 48-49 39.36977976531114 40.0 40.0 40.0 40.0 40.0 50-51 39.32382120843613 40.0 40.0 40.0 40.0 40.0 52-53 39.28132300923344 40.0 40.0 40.0 40.0 40.0 54-55 39.18641798636956 40.0 40.0 40.0 40.0 40.0 56-57 39.21039801574125 40.0 40.0 40.0 40.0 40.0 58-59 39.15538534427405 40.0 40.0 40.0 40.0 40.0 60-61 39.06894425681903 40.0 40.0 40.0 40.0 40.0 62-63 39.39790330342932 40.0 40.0 40.0 40.0 40.0 64-65 39.39813022468721 40.0 40.0 40.0 40.0 40.0 66-67 39.391238527447186 40.0 40.0 40.0 40.0 40.0 68-69 39.39449465585982 40.0 40.0 40.0 40.0 40.0 70-71 39.393914644438645 40.0 40.0 40.0 40.0 40.0 72-73 39.3901973303342 40.0 40.0 40.0 40.0 40.0 74-75 39.401452383210966 40.0 40.0 40.0 40.0 40.0 76-77 39.392621379657534 40.0 40.0 40.0 40.0 40.0 78-79 39.39504910697384 40.0 40.0 40.0 40.0 40.0 80-81 39.37856799730595 40.0 40.0 40.0 40.0 40.0 82-83 39.385649574579446 40.0 40.0 40.0 40.0 40.0 84-85 39.386035482267 40.0 40.0 40.0 40.0 40.0 86-87 39.347476460729105 40.0 40.0 40.0 40.0 40.0 88-89 39.40908434633662 40.0 40.0 40.0 40.0 40.0 90-91 39.443907104165994 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06300947975415738 1101 2 -0.009588433452037748 1101 3 0.08770841044164968 1101 4 -0.016521299160885405 1101 5 0.023619425015361628 1101 6 -0.05709733091164537 1101 7 -0.1950112759283371 1101 8 -0.017012528952768946 1101 9 -0.10523772450407165 1101 10-11 -0.049322558319587984 1101 12-13 4.250080138490375E-6 1101 14-15 -0.11027182871924879 1101 16-17 -0.008734259743860662 1101 18-19 -0.033154572172144015 1101 20-21 -0.041604003403762135 1101 22-23 -0.09821926483702015 1101 24-25 -0.021469362337100506 1101 26-27 0.028464225162927903 1101 28-29 0.025463418326744147 1101 30-31 -0.03813289309517387 1101 32-33 0.03741350609614358 1101 34-35 0.033479353882256646 1101 36-37 -0.06794121668983166 1101 38-39 0.012164013943468888 1101 40-41 -0.057745706087168 1101 42-43 -0.022489930344576692 1101 44-45 -0.03930688111496039 1101 46-47 0.025460931055974356 1101 48-49 -0.0242048586930963 1101 50-51 0.06266098661089359 1101 52-53 -0.09479126060396936 1101 54-55 0.007411136844659438 1101 56-57 -0.032501652100542344 1101 58-59 0.008808901438790429 1101 60-61 -0.12306768097876386 1101 62-63 -0.00967422157918918 1101 64-65 -0.07527608369336036 1101 66-67 -0.11989124645834437 1101 68-69 -0.11446725085731657 1101 70-71 0.1298837859277029 1101 72-73 -0.3170321229477082 1101 74-75 -0.1974343365124014 1101 76-77 -0.13213993125093992 1101 78-79 -0.6862620468162888 1101 80-81 -0.06911492033904665 1101 82-83 -0.3299712485668209 1101 84-85 0.05909046592756084 1101 86-87 0.11380037270749455 1101 88-89 0.3593506055142086 1101 90-91 0.36358374676814265 1102 1 0.04610813824827176 1102 2 0.13996793439110888 1102 3 0.23233521392770484 1102 4 -0.02285256073133013 1102 5 0.017730578602147773 1102 6 -0.0531270486106834 1102 7 0.045079841549352295 1102 8 -0.14533010572039018 1102 9 -0.08514148131032329 1102 10-11 0.010366262035518048 1102 12-13 -0.0941643172535862 1102 14-15 -0.060830861075693576 1102 16-17 -0.052740250245534526 1102 18-19 -0.020529274902088446 1102 20-21 -0.0605977881150892 1102 22-23 -0.10755078842032617 1102 24-25 0.02070396990809087 1102 26-27 -0.059048785994811226 1102 28-29 0.0373549364120791 1102 30-31 -0.02241641678156725 1102 32-33 -0.004014329909331593 1102 34-35 -0.028070570981334697 1102 36-37 0.0953825920971525 1102 38-39 0.08187207076644398 1102 40-41 -0.026864455888869543 1102 42-43 -0.11727260531340988 1102 44-45 0.04595000244784586 1102 46-47 0.16471170403808344 1102 48-49 -0.1096017847881825 1102 50-51 -0.026980930612658938 1102 52-53 -0.02420136222738023 1102 54-55 -0.03557514443257759 1102 56-57 0.06316451648935839 1102 58-59 0.07965730206342414 1102 60-61 0.2466923913157757 1102 62-63 0.09673780338629001 1102 64-65 0.019934421743265318 1102 66-67 0.060899867303398025 1102 68-69 0.05412375213302312 1102 70-71 0.05238811218679729 1102 72-73 -0.006942508819641091 1102 74-75 0.08364710743829562 1102 76-77 0.1816865958587428 1102 78-79 0.03489616005620633 1102 80-81 -0.27560642646641753 1102 82-83 -0.13771275295780327 1102 84-85 0.09122544010714506 1102 86-87 -0.14008628473334994 1102 88-89 0.3593506055142086 1102 90-91 0.36358374676814265 1103 1 0.02404797442202522 1103 2 -0.0024792290296034025 1103 3 0.007806776070474086 1103 4 -0.010109891157597417 1103 5 -0.04781829019979256 1103 6 0.05738431141283229 1103 7 0.027401471729326943 1103 8 0.027011038350345018 1103 9 -0.010234140498312172 1103 10-11 -0.08707743240606192 1103 12-13 -0.11699175426441144 1103 14-15 -0.026424381503098004 1103 16-17 -0.0823650494191952 1103 18-19 -0.12258367358175803 1103 20-21 -0.04021629932250903 1103 22-23 -0.05504768329542031 1103 24-25 -0.07295210801182606 1103 26-27 -0.04225991611897939 1103 28-29 -0.034868361280025795 1103 30-31 -0.11146391000257694 1103 32-33 -0.03723327551563216 1103 34-35 -0.08675820428158687 1103 36-37 0.04875609108264456 1103 38-39 0.033827417064330234 1103 40-41 -0.011899185110706867 1103 42-43 0.02178104351006027 1103 44-45 0.03723490717629119 1103 46-47 -0.08667464228959432 1103 48-49 -0.09820227675540849 1103 50-51 0.053829007435879817 1103 52-53 0.015569179306645253 1103 54-55 -0.14702402162806294 1103 56-57 -0.02100171361085046 1103 58-59 -0.007209653587288756 1103 60-61 0.05348043114121026 1103 62-63 -0.1760155825378149 1103 64-65 0.01916994926139637 1103 66-67 0.18466211286524725 1103 68-69 0.0168781447059132 1103 70-71 -0.07127063767364206 1103 72-73 0.016022283282197236 1103 74-75 0.08856511101811293 1103 76-77 -0.30140306896363 1103 78-79 0.046467263813433135 1103 80-81 -0.238201705014788 1103 82-83 -0.5226419312977413 1103 84-85 0.11117379926089654 1103 86-87 0.2890107052332027 1103 88-89 0.3593506055142086 1103 90-91 0.36358374676814265 1104 1 -0.040959938657564976 1104 2 -0.10778281629234954 1104 3 0.009298195149419541 1104 4 -0.23139130496115 1104 5 -0.13748723164300714 1104 6 -0.04079119059069569 1104 7 -0.10888869697079429 1104 8 -0.25312600945363783 1104 9 -0.22174346221831343 1104 10-11 -0.10094777014303702 1104 12-13 -1.2440778377253991E-4 1104 14-15 -0.027428248249087517 1104 16-17 -0.01076079880466807 1104 18-19 -0.12653530277821545 1104 20-21 -0.018761190615876444 1104 22-23 -0.02561144812543148 1104 24-25 -0.09855106079150033 1104 26-27 -0.09377069698840756 1104 28-29 -0.06192014986049088 1104 30-31 -0.08701460067822353 1104 32-33 0.018979594162146896 1104 34-35 -0.0066874411809507706 1104 36-37 -0.10022731827297093 1104 38-39 -0.009597536529525996 1104 40-41 -0.17209920829239422 1104 42-43 -0.05914147467181152 1104 44-45 -0.13693248968713334 1104 46-47 -0.18297797773465163 1104 48-49 -0.16247401703963504 1104 50-51 -0.06604838459698925 1104 52-53 0.012361261855183159 1104 54-55 -0.0837151651005783 1104 56-57 -0.09501033290658256 1104 58-59 -0.15932751218727503 1104 60-61 -0.12517250636378208 1104 62-63 -0.01936378070665512 1104 64-65 0.03681806143097077 1104 66-67 -0.11684372244019414 1104 68-69 0.06158573507323695 1104 70-71 0.08520331215572696 1104 72-73 -0.21879045841959055 1104 74-75 -0.2634195716582681 1104 76-77 -0.03965925371924328 1104 78-79 0.16327177380416202 1104 80-81 -0.009403695373976007 1104 82-83 -0.5439865875079377 1104 84-85 -0.5909362243459029 1104 86-87 -0.46059910524617464 1104 88-89 0.063054309217911 1104 90-91 -0.30308291989852876 1201 1 0.029490632159607344 1201 2 0.04421762363335091 1201 3 0.07419130949751462 1201 4 0.17609530717645328 1201 5 -0.03831521526255699 1201 6 0.18832591654479103 1201 7 3.703812490840619E-4 1201 8 0.027690514372956443 1201 9 0.04885972479898726 1201 10-11 0.025248447582171707 1201 12-13 0.06468520214878026 1201 14-15 0.08436651283323471 1201 16-17 0.00931352691414844 1201 18-19 0.05629523892782373 1201 20-21 0.09064996068597964 1201 22-23 0.05870153457082239 1201 24-25 0.016885307486056433 1201 26-27 0.03502705667492734 1201 28-29 -0.020869922293989873 1201 30-31 0.05693655069860881 1201 32-33 0.01125446967235888 1201 34-35 0.1057213391865588 1201 36-37 0.027135313529129235 1201 38-39 0.01492219109206161 1201 40-41 0.026114157386373904 1201 42-43 0.13605565432301603 1201 44-45 0.028195779029083212 1201 46-47 -0.08654583645626701 1201 48-49 0.06328264639572723 1201 50-51 -0.0037256758256631883 1201 52-53 -0.08234121767080183 1201 54-55 -0.09258113566621518 1201 56-57 -0.11633177451410148 1201 58-59 -0.19834776839733337 1201 60-61 0.10724096596858601 1201 62-63 0.10313173698900613 1201 64-65 -0.034808460047038636 1201 66-67 0.11445025296526268 1201 68-69 0.012726260753019858 1201 70-71 0.06760555043099004 1201 72-73 -0.11515691875173673 1201 74-75 0.06670425636497157 1201 76-77 0.1677238164945365 1201 78-79 0.13360797455749207 1201 80-81 -0.5340234136838404 1201 82-83 0.28561404890098174 1201 84-85 0.2103801484672445 1201 86-87 -0.3566058307610973 1201 88-89 -0.43012307869631883 1201 90-91 0.36358374676814265 1202 1 0.09783868189487066 1202 2 0.20273588583319224 1202 3 0.05599133438740722 1202 4 -0.0651626239473444 1202 5 0.10488463337451748 1202 6 0.02250257205147932 1202 7 -0.008132653597783701 1202 8 0.07118509576239518 1202 9 0.0905776130433722 1202 10-11 0.03369482251096656 1202 12-13 0.0266279493251389 1202 14-15 0.022696239155713727 1202 16-17 -0.03645756904043651 1202 18-19 -0.032298613673418686 1202 20-21 -0.050445376708658785 1202 22-23 -1.3574320341547264E-4 1202 24-25 -0.050175752681717256 1202 26-27 0.039153112851209926 1202 28-29 0.043675842225361805 1202 30-31 -0.03357766841136112 1202 32-33 -0.014044217309248097 1202 34-35 -0.04285507234113339 1202 36-37 -0.12443992342731747 1202 38-39 -0.06141084376606187 1202 40-41 -0.10732630357433948 1202 42-43 -0.025190314047890183 1202 44-45 -0.021573071626299622 1202 46-47 -0.058020002446113494 1202 48-49 -0.16047394525903513 1202 50-51 -0.010969397630638866 1202 52-53 -0.11819564175004871 1202 54-55 -0.22651486330337178 1202 56-57 -0.13512788771518558 1202 58-59 0.026162278167234376 1202 60-61 -0.11223211948161804 1202 62-63 -0.21489026294742786 1202 64-65 0.03783088138664681 1202 66-67 -0.28152233781165137 1202 68-69 -0.18523235146501094 1202 70-71 -0.2691268010890653 1202 72-73 -0.2615362754347643 1202 74-75 -0.001599820128589613 1202 76-77 -0.18302623693629982 1202 78-79 -0.1661377260210486 1202 80-81 -0.22656787447846227 1202 82-83 0.20526481166924526 1202 84-85 -0.30366136557426415 1202 86-87 -0.43265239326817095 1202 88-89 0.10128608938517658 1202 90-91 -1.2364162532318588 1203 1 0.019006842393380907 1203 2 0.006283298186950503 1203 3 -0.07814411022371814 1203 4 -0.11825364914454184 1203 5 -0.07815495444567233 1203 6 -0.12081541959314279 1203 7 0.04344156297022295 1203 8 -0.10332006074018807 1203 9 -0.05396036112527014 1203 10-11 0.02793462600664043 1203 12-13 -0.0016215902029443896 1203 14-15 -0.08135764975983761 1203 16-17 -0.027062614335349622 1203 18-19 -0.018693629990345073 1203 20-21 -0.0225709545749595 1203 22-23 -0.05555304088084512 1203 24-25 0.0449393133876157 1203 26-27 0.04201312768570631 1203 28-29 -0.05133914715255372 1203 30-31 0.04430582529997196 1203 32-33 -0.09574650092112336 1203 34-35 -0.07712483938596648 1203 36-37 0.1081290198908107 1203 38-39 -0.007704711720499802 1203 40-41 -0.10293047289511748 1203 42-43 -0.10891717195300998 1203 44-45 -0.16360981143063924 1203 46-47 -0.133492654589773 1203 48-49 -0.04037124412013071 1203 50-51 -0.07401427291758012 1203 52-53 -0.05879400747041785 1203 54-55 -0.1679667574414836 1203 56-57 -0.24323205868527253 1203 58-59 -0.01350963810273953 1203 60-61 -0.15534756259581428 1203 62-63 -0.02614478470038506 1203 64-65 -0.05999155657580957 1203 66-67 -0.20180687680826992 1203 68-69 -0.07491761943708752 1203 70-71 -0.17851582965887758 1203 72-73 -0.5185414565919402 1203 74-75 -0.15831903562607152 1203 76-77 -0.011048936930585285 1203 78-79 -0.2659954993104563 1203 80-81 0.011216925246642973 1203 82-83 0.13364990270493848 1203 84-85 0.3472849103720037 1203 86-87 0.37877227606069397 1203 88-89 0.3593506055142086 1203 90-91 0.36358374676814265 1204 1 0.006454375470845264 1204 2 0.006007828865399745 1204 3 -0.36186668215962214 1204 4 -0.2835541932810557 1204 5 -0.42020527869743063 1204 6 -0.2671430988114807 1204 7 -0.09767453448906593 1204 8 -0.16324512902374977 1204 9 -0.0951074911850327 1204 10-11 -0.23550947825613378 1204 12-13 -0.10924752181548314 1204 14-15 -0.12385369961775439 1204 16-17 -0.13439886322154848 1204 18-19 -0.026884612126465868 1204 20-21 -0.07042545804151246 1204 22-23 0.01776376203709873 1204 24-25 -0.006206608532735913 1204 26-27 -0.049988211081661404 1204 28-29 -0.04215525716700341 1204 30-31 -0.0011134481446148925 1204 32-33 -0.18328705597333794 1204 34-35 -0.09918480149570996 1204 36-37 -0.06601462267018121 1204 38-39 -0.05952514853252211 1204 40-41 -0.03175311027050043 1204 42-43 -0.07717031252751383 1204 44-45 -0.08015214206478305 1204 46-47 -0.11409780589082175 1204 48-49 -0.08462292579093145 1204 50-51 -0.1503627337148501 1204 52-53 0.012466005029637017 1204 54-55 -0.06839067121457987 1204 56-57 -0.079135248346212 1204 58-59 -0.04987791048579737 1204 60-61 -0.10825258606739396 1204 62-63 -0.025180353092991936 1204 64-65 -0.009941848446338497 1204 66-67 0.09578233075189502 1204 68-69 -0.10621758184547758 1204 70-71 -0.18157432350104585 1204 72-73 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0.12942289953393527 2401 42-43 0.15122490328799643 2401 44-45 0.0782420051767545 2401 46-47 0.08711295903960803 2401 48-49 0.08571842183010148 2401 50-51 0.05303381964039744 2401 52-53 0.11606881634999411 2401 54-55 0.1111781857776819 2401 56-57 0.13883874113165717 2401 58-59 0.08108827683317799 2401 60-61 0.2688968864715946 2401 62-63 0.18108081243698138 2401 64-65 0.28345074035606643 2401 66-67 0.20843892886052373 2401 68-69 0.3323091569605481 2401 70-71 0.14739681480374855 2401 72-73 0.15389492345219935 2401 74-75 0.35785645037676517 2401 76-77 0.23998500220478292 2401 78-79 0.21928174302669134 2401 80-81 0.332048600295181 2401 82-83 0.36385480726710995 2401 84-85 0.3804481756781328 2401 86-87 0.039343057853884034 2401 88-89 -0.004285758122151151 2401 90-91 0.36358374676814265 2402 1 0.07758405098913812 2402 2 0.0891876428981675 2402 3 0.09078202207901853 2402 4 0.1393888612280989 2402 5 0.06271419193440408 2402 6 0.089755355813665 2402 7 0.14241138273060017 2402 8 0.0875205188009005 2402 9 0.11492786027247348 2402 10-11 0.0450261256769835 2402 12-13 0.07112992252721995 2402 14-15 0.10465560787805117 2402 16-17 0.0618695642706939 2402 18-19 0.06064806172546611 2402 20-21 0.09990314024750546 2402 22-23 0.023188394839145587 2402 24-25 0.009991291425535564 2402 26-27 -0.02517423068000113 2402 28-29 0.08907749855376323 2402 30-31 0.052030415212122705 2402 32-33 0.06023611228827974 2402 34-35 0.03682508459294809 2402 36-37 -1.6341120874585613E-4 2402 38-39 -0.20153234774999618 2402 40-41 0.10294036583581345 2402 42-43 0.12592246662906348 2402 44-45 0.13890762319682182 2402 46-47 0.20968305486551486 2402 48-49 0.15098620781540006 2402 50-51 0.12560477709850204 2402 52-53 0.0434957500148343 2402 54-55 0.07978135730370184 2402 56-57 0.2487954741347167 2402 58-59 0.2692611544357604 2402 60-61 0.2472189398589677 2402 62-63 0.19379071743703236 2402 64-65 0.06035124599391395 2402 66-67 0.10876074166356631 2402 68-69 -0.03569435372126861 2402 70-71 -0.07334024081340118 2402 72-73 0.07921890698616352 2402 74-75 0.034323937533415005 2402 76-77 -0.03040390165138973 2402 78-79 -0.20783214612037426 2402 80-81 0.3613960899185571 2402 82-83 -0.06611628673476133 2402 84-85 0.40562705505436014 2402 86-87 0.3405404958934284 2402 88-89 -0.09898272781911999 2402 90-91 -0.8278141026942194 2403 1 0.08615534789168322 2403 2 0.05405033398420045 2403 3 0.08939567288840067 2403 4 0.11850180295083135 2403 5 0.14653093020534413 2403 6 0.16643236097247893 2403 7 0.04871142855385813 2403 8 -0.02248034201551974 2403 9 0.02299823157998304 2403 10-11 0.10736627685025724 2403 12-13 0.19868065635148469 2403 14-15 0.074624766303927 2403 16-17 0.041408914449945655 2403 18-19 0.08813784279115566 2403 20-21 0.07739461686068694 2403 22-23 0.09399932738157446 2403 24-25 0.042777441150207096 2403 26-27 0.06705972704956764 2403 28-29 0.09233886241738531 2403 30-31 0.1330445439050436 2403 32-33 0.057356306411193714 2403 34-35 0.03573793891764154 2403 36-37 0.04260577430725476 2403 38-39 0.09267622788689067 2403 40-41 0.02382436209502714 2403 42-43 0.04296195838907835 2403 44-45 -0.03401928634629314 2403 46-47 0.06859332378969896 2403 48-49 0.11179189644671794 2403 50-51 0.12828436671540544 2403 52-53 0.07199430408729768 2403 54-55 -0.00599755836366711 2403 56-57 -0.09176441769088939 2403 58-59 -0.038883854900852555 2403 60-61 -0.15581810920849648 2403 62-63 -0.04567598122128658 2403 64-65 0.01824545243978548 2403 66-67 0.15520211174436582 2403 68-69 0.12662718091672787 2403 70-71 0.07052353665810784 2403 72-73 0.03663365484642611 2403 74-75 0.05576202654635409 2403 76-77 0.17886785766911117 2403 78-79 0.19911108370933306 2403 80-81 0.23836754095907509 2403 82-83 -0.0781855342811042 2403 84-85 0.13093018666434375 2403 86-87 -0.10572343454622057 2403 88-89 -0.3069427875429014 2403 90-91 0.06728745047184503 2404 1 -0.025414580797246344 2404 2 -0.1980184683977555 2404 3 -0.06684806080835415 2404 4 -0.024790485644174964 2404 5 -0.08979648441016508 2404 6 -0.1280307226346551 2404 7 -0.03216045023033587 2404 8 -0.13038011588606224 2404 9 0.02890450721486104 2404 10-11 -0.1344038697215595 2404 12-13 -0.07062259583277353 2404 14-15 0.06048928828536049 2404 16-17 -0.06926170478016758 2404 18-19 -0.0713993079777282 2404 20-21 -0.08144287809372486 2404 22-23 -0.026999461675679015 2404 24-25 -0.08104948518194277 2404 26-27 -0.06515959681902928 2404 28-29 0.04635081559425913 2404 30-31 0.03687768683278847 2404 32-33 -0.07407407731812299 2404 34-35 -0.025838850194844554 2404 36-37 0.026702196644258436 2404 38-39 0.08163317000204984 2404 40-41 -0.08153305391471122 2404 42-43 0.0576361446889706 2404 44-45 0.002809194966125972 2404 46-47 0.03721717109489475 2404 48-49 0.1369549885463286 2404 50-51 -9.379376900326974E-4 2404 52-53 -0.03522475720917839 2404 54-55 0.06174238891498618 2404 56-57 -0.005416054325635855 2404 58-59 -0.04948205441014153 2404 60-61 0.05601848048453917 2404 62-63 -0.2069462999286813 2404 64-65 -0.14973365640891245 2404 66-67 -0.08716224449784704 2404 68-69 0.05083531021716681 2404 70-71 -0.22651385795386858 2404 72-73 0.015457441730070798 2404 74-75 -0.22899721810310325 2404 76-77 0.03018121517922623 2404 78-79 0.22730724359632148 2404 80-81 0.32982720081351147 2404 82-83 0.1949266411540762 2404 84-85 0.20367187103622797 2404 86-87 0.517433807115097 2404 88-89 -0.10924022640429598 2404 90-91 0.36358374676814265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 16.0 31 423.0 32 2569.0 33 4585.0 34 6287.0 35 7959.0 36 11158.0 37 16610.0 38 42884.0 39 265194.0 40 278634.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.816098818204676 10.426043500125722 33.085711591652 30.6721460900176 2 27.023543631714038 16.77823656170871 29.13695355244938 27.061266254127876 3 27.84432361076188 18.9073107870254 21.0475861201911 32.20077948202162 4 26.40923813370338 19.80091746435357 20.11695391776766 33.67289048417539 5 27.335617299471966 20.00298591903445 20.29953482524516 32.361861956248426 6 28.325999497108374 22.256254714609003 23.30006914759869 26.117676640683936 7 26.128363087754593 21.74016218254966 23.013106613024892 29.118368116670858 8 25.212314558712595 22.84290922806135 23.453451093789287 28.491325119436762 9 25.73406462157405 23.08398290168469 23.549471963791802 27.63248051294946 10-11 25.11770807141061 21.791394267035454 23.6241985164697 29.466699145084235 12-13 25.73563615790797 21.658913754085994 23.41573422177521 29.189715866230827 14-15 26.120505406084987 21.29871762635152 23.172460397284386 29.4083165702791 16-17 26.040828513955244 21.002875911491074 22.99904136283631 29.957254211717377 18-19 26.36613653507669 21.306103847120944 22.878975986924818 29.448783630877546 20-21 26.365665074176516 21.45005657530802 22.751131506160423 29.43314684435504 22-23 25.999889992456627 20.973016721146593 23.133722026653256 29.893371259743525 24-25 26.12121259743525 21.433712597435253 23.13458637163691 29.310488433492583 26-27 26.325590897661556 21.430569524767414 23.102841337691725 29.140998239879305 28-29 26.122469826502385 21.12930600955494 23.264866733718883 29.48335743022379 30-31 26.40472349497139 21.333991295102926 23.21334698499234 29.047938224933347 32-33 26.373046347743053 21.462111535966542 23.30304727445616 28.86179484183425 34-35 26.15614871561353 21.039613076902473 23.629061420191253 29.175176787292745 36-37 26.41831741810498 21.29248614138108 23.60095919739342 28.688237243120522 38-39 26.47338254562428 21.374360727195214 23.504289996012297 28.64796673116821 40-41 26.118326118326117 21.01972101972102 23.743871122903382 29.11808173904948 42-43 26.33789615713418 21.374615043928767 23.7609821915974 28.52650660733966 44-45 26.486506108386738 21.261321910771134 23.769577528000898 28.48259445284123 46-47 26.164894038224478 21.046510665908684 23.88953170225515 28.899063593611686 48-49 26.37539109360568 21.38906739266245 23.82627203634408 28.409269477387795 50-51 26.573142288678703 21.396927320430837 23.769214653293353 28.260715737597103 52-53 26.38226243630904 21.05396327097692 23.99704252730587 28.566731765408175 54-55 26.48155822627435 21.521976842304984 23.865707338263125 28.130757593157547 56-57 26.672805909848705 21.42614251499427 23.758319685464883 28.142731889692136 58-59 26.310721011321466 21.20677941203474 23.99929814682197 28.483201429821825 60-61 26.734681320884995 21.26122595008967 23.881392895150025 28.12269983387531 62-63 26.77782962989554 21.27805783619403 23.789096354340277 28.155016179570154 64-65 26.558774214883705 21.13504638466492 23.96773089352123 28.338448506930142 66-67 26.81445671429281 21.36388470389563 23.79861260575156 28.02304597606 68-69 26.75885326268993 21.37952559300874 23.680384275752868 28.18123686854846 70-71 26.594349164565013 21.24297178999068 23.923518941384838 28.23916010405947 72-73 26.78486311731913 21.311628292541396 23.6632955463909 28.240213043748575 74-75 26.882150325993358 21.499569442735883 23.68372493541641 27.934555295854345 76-77 26.831381785055658 21.108568296783464 23.814073366365232 28.24597655179565 78-79 26.857730158441417 21.548760300511162 23.47061284630637 28.122896694741055 80-81 27.347377570392734 21.21960703584254 23.296507239415355 28.136508154349364 82-83 27.43078675959219 20.607863156804427 23.45777659401311 28.503573489590266 84-85 28.020239079162213 20.368093212651438 23.004817868445983 28.606849839740363 86-87 28.793017015127838 19.616053787856448 22.632774026127215 28.9581551708885 88-89 27.501239464551315 19.32077342588002 23.425880019831432 29.752107089737233 90-91 22.449232293214465 20.49281822684497 27.897473997028232 29.160475482912336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 3.0 10 6.0 11 7.5 12 6.0 13 8.5 14 18.0 15 30.5 16 42.0 17 56.5 18 81.0 19 113.0 20 159.5 21 213.0 22 298.5 23 438.0 24 611.5 25 803.0 26 1043.5 27 1359.0 28 1754.5 29 2165.0 30 2655.0 31 3311.0 32 3950.0 33 4559.5 34 5431.0 35 6490.5 36 7658.0 37 8902.5 38 10239.5 39 11842.0 40 13656.5 41 15612.5 42 17665.5 43 19903.5 44 22430.0 45 25068.5 46 27800.0 47 30688.5 48 33477.5 49 36357.5 50 39258.0 51 41951.5 52 44150.5 53 45854.5 54 47288.5 55 48233.5 56 48750.5 57 48873.5 58 48635.5 59 48062.0 60 47185.0 61 45796.0 62 44058.0 63 42143.5 64 39556.5 65 36480.0 66 33195.5 67 30230.5 68 27294.5 69 23960.0 70 20638.5 71 17506.0 72 14665.0 73 11983.5 74 9564.0 75 7630.5 76 6049.5 77 4618.5 78 3557.5 79 2712.0 80 1933.5 81 1390.5 82 1018.5 83 718.0 84 517.5 85 380.5 86 262.0 87 181.0 88 132.5 89 94.5 90 64.5 91 43.0 92 25.5 93 14.5 94 11.0 95 12.0 96 11.5 97 8.5 98 5.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01524390243902439 3 0.0 4 1.5715363339200404E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.06005509641873278 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.5641091387413814E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.416766043902655E-4 72-73 5.180998171107645E-4 74-75 0.0 76-77 0.0 78-79 9.095291367658963E-4 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10326.0 32-33 22598.0 34-35 24996.0 36-37 26723.0 38-39 28730.0 40-41 29975.0 42-43 30493.0 44-45 31473.0 46-47 31839.0 48-49 31030.0 50-51 30714.0 52-53 29895.0 54-55 28525.0 56-57 27339.0 58-59 26070.0 60-61 24716.0 62-63 23025.0 64-65 21764.0 66-67 19967.0 68-69 18564.0 70-71 17032.0 72-73 15651.0 74-75 14102.0 76-77 12824.0 78-79 11582.0 80-81 10199.0 82-83 9304.0 84-85 8044.0 86-87 7146.0 88-89 6237.0 90-91 5437.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88313904290945 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8907680403558 99.77403473277874 2 0.1048340807265349 0.20942314123288164 3 0.002744441223003838 0.008223702128171546 4 6.351911700327544E-4 0.002537795518208398 5 5.475421814347724E-4 0.002734511592005366 6 2.452497662844336E-4 0.0014697789903617476 7 2.2545457689083376E-4 0.0015763377596013225 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0023573045008800603 0.0 0.0 0.0 0.0 2 0.002514458134272064 0.0 0.0 0.0 1.57153633392004E-4 3 0.002514458134272064 0.0 0.0 0.0 1.57153633392004E-4 4 0.002514458134272064 0.0 0.0 0.0 1.57153633392004E-4 5 0.0028287654010560725 0.0 0.0 0.0 1.57153633392004E-4 6 0.0029859190344480764 0.0 0.0 0.0 1.57153633392004E-4 7 0.0031430726678400803 0.0 0.0 0.0 1.57153633392004E-4 8 0.005814684435504149 0.0 0.0 0.0 3.14307266784008E-4 9 0.005971838068896153 0.0 0.0 0.0 3.14307266784008E-4 10-11 0.005971838068896153 0.0 0.0 0.0 3.14307266784008E-4 12-13 0.005971838068896153 0.0 0.0 0.0 3.14307266784008E-4 14-15 0.005971838068896153 0.0 0.0 0.0 3.14307266784008E-4 16-17 0.006128991702288157 0.0 0.0 0.0 3.14307266784008E-4 18-19 0.006128991702288157 0.0 0.0 0.0 3.14307266784008E-4 20-21 0.006128991702288157 0.0 0.0 0.0 3.14307266784008E-4 22-23 0.006364722152376163 0.0 0.0 0.0 3.14307266784008E-4 24-25 0.006836183052552175 0.0 0.0 0.0 3.14307266784008E-4 26-27 0.007229067136032185 0.0 0.0 0.0 3.14307266784008E-4 28-29 0.007700528036208197 0.0 0.0 0.0 3.14307266784008E-4 30-31 0.007857681669600202 0.0 0.0 0.0 3.14307266784008E-4 32-33 0.008093412119688208 0.0 0.0 0.0 4.7146090017601206E-4 34-35 0.008329142569776214 1.57153633392004E-4 0.0 0.0 4.7146090017601206E-4 36-37 0.008486296203168217 1.57153633392004E-4 0.0 0.0 4.7146090017601206E-4 38-39 0.008800603469952225 1.57153633392004E-4 0.0 0.0 4.7146090017601206E-4 40-41 0.008879180286648226 2.35730450088006E-4 0.0 0.0 4.7146090017601206E-4 42-43 0.00895775710334423 3.14307266784008E-4 0.0 0.0 4.7146090017601206E-4 44-45 0.009272064370128237 3.14307266784008E-4 0.0 0.0 4.7146090017601206E-4 46-47 0.009272064370128237 3.14307266784008E-4 0.0 0.0 5.500377168720141E-4 48-49 0.009272064370128237 4.7146090017601206E-4 0.0 0.0 6.28614533568016E-4 50-51 0.009272064370128237 4.7146090017601206E-4 0.0 0.0 6.28614533568016E-4 52-53 0.009586371636912245 4.7146090017601206E-4 0.0 0.0 6.28614533568016E-4 54-55 0.009743525270304249 4.7146090017601206E-4 7.8576816696002E-5 0.0 6.28614533568016E-4 56-57 0.00982210208700025 4.7146090017601206E-4 1.57153633392004E-4 0.0 6.28614533568016E-4 58-59 0.009900678903696253 4.7146090017601206E-4 1.57153633392004E-4 0.0 6.28614533568016E-4 60-61 0.01021498617048026 4.7146090017601206E-4 1.57153633392004E-4 0.0 6.28614533568016E-4 62-63 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