##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-21B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 561209 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.57260842217427 40.0 40.0 40.0 40.0 40.0 2 39.36621472570825 40.0 40.0 40.0 40.0 40.0 3 39.3501102084963 40.0 40.0 40.0 40.0 40.0 4 39.186285323293106 40.0 40.0 40.0 40.0 40.0 5 39.24030441422001 40.0 40.0 40.0 40.0 40.0 6 39.36997446584071 40.0 40.0 40.0 40.0 40.0 7 39.403167091048076 40.0 40.0 40.0 40.0 40.0 8 39.42295294622859 40.0 40.0 40.0 40.0 40.0 9 39.403366660192546 40.0 40.0 40.0 40.0 40.0 10-11 39.41640280180824 40.0 40.0 40.0 40.0 40.0 12-13 39.33789016213211 40.0 40.0 40.0 40.0 40.0 14-15 39.37407632450656 40.0 40.0 40.0 40.0 40.0 16-17 39.47445604044126 40.0 40.0 40.0 40.0 40.0 18-19 39.502445612953466 40.0 40.0 40.0 40.0 40.0 20-21 39.47890714510993 40.0 40.0 40.0 40.0 40.0 22-23 39.51941968143775 40.0 40.0 40.0 40.0 40.0 24-25 39.496437156210966 40.0 40.0 40.0 40.0 40.0 26-27 39.47628423635402 40.0 40.0 40.0 40.0 40.0 28-29 39.48992621287257 40.0 40.0 40.0 40.0 40.0 30-31 39.47217613105379 40.0 40.0 40.0 40.0 40.0 32-33 39.47007032715857 40.0 40.0 40.0 40.0 40.0 34-35 39.46668746819836 40.0 40.0 40.0 40.0 40.0 36-37 39.44604539308397 40.0 40.0 40.0 40.0 40.0 38-39 39.38664152584365 40.0 40.0 40.0 40.0 40.0 40-41 39.42335239009934 40.0 40.0 40.0 40.0 40.0 42-43 39.41110021014059 40.0 40.0 40.0 40.0 40.0 44-45 39.30757359166037 40.0 40.0 40.0 40.0 40.0 46-47 39.29853759683658 40.0 40.0 40.0 40.0 40.0 48-49 39.377676613777524 40.0 40.0 40.0 40.0 40.0 50-51 39.340756885566144 40.0 40.0 40.0 40.0 40.0 52-53 39.298163813478965 40.0 40.0 40.0 40.0 40.0 54-55 39.21676533008524 40.0 40.0 40.0 40.0 40.0 56-57 39.22920888871457 40.0 40.0 40.0 40.0 40.0 58-59 39.177203425009 40.0 40.0 40.0 40.0 40.0 60-61 39.079824837491095 40.0 40.0 40.0 40.0 40.0 62-63 39.386400589727494 40.0 40.0 40.0 40.0 40.0 64-65 39.39031877070118 40.0 40.0 40.0 40.0 40.0 66-67 39.39252043384911 40.0 40.0 40.0 40.0 40.0 68-69 39.40004176607599 40.0 40.0 40.0 40.0 40.0 70-71 39.392831231171265 40.0 40.0 40.0 40.0 40.0 72-73 39.380858072944356 40.0 40.0 40.0 40.0 40.0 74-75 39.39462914439221 40.0 40.0 40.0 40.0 40.0 76-77 39.39862372133311 40.0 40.0 40.0 40.0 40.0 78-79 39.38816629303188 40.0 40.0 40.0 40.0 40.0 80-81 39.39594692951316 40.0 40.0 40.0 40.0 40.0 82-83 39.36915511561428 40.0 40.0 40.0 40.0 40.0 84-85 39.37073249961814 40.0 40.0 40.0 40.0 40.0 86-87 39.34210563464669 40.0 40.0 40.0 40.0 40.0 88-89 39.33915881920791 40.0 40.0 40.0 40.0 40.0 90-91 39.35968274787394 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06888241883029167 1101 2 -0.13132358784236686 1101 3 -0.027837424140024325 1101 4 -0.010390781082456613 1101 5 -0.03678412018462751 1101 6 0.14007448720157356 1101 7 -0.07695001276282909 1101 8 0.0045137353047906 1101 9 -0.06364829892684298 1101 10-11 -0.04455065510974521 1101 12-13 0.0913723284854484 1101 14-15 0.011729591332617417 1101 16-17 0.05239863042909576 1101 18-19 0.032142494495730034 1101 20-21 0.006194903157982878 1101 22-23 0.008938212113513089 1101 24-25 0.028839690750707803 1101 26-27 0.046497798499743226 1101 28-29 0.035782764977660975 1101 30-31 0.003977564318631721 1101 32-33 0.04348505386825252 1101 34-35 0.08124246929593681 1101 36-37 -0.12697304006072585 1101 38-39 -0.004249136464444803 1101 40-41 -0.10410495009580245 1101 42-43 0.017029826257378033 1101 44-45 0.017490831739777946 1101 46-47 -0.04054066549852564 1101 48-49 -0.004824455914246073 1101 50-51 0.1500872674144773 1101 52-53 0.12676829826289548 1101 54-55 0.24798043435599482 1101 56-57 0.12207604670062011 1101 58-59 -0.024882840616463398 1101 60-61 0.23925727723785428 1101 62-63 -0.024479263036965904 1101 64-65 0.041764210727457396 1101 66-67 0.17515028569649616 1101 68-69 0.1537727691450712 1101 70-71 0.1426256949602802 1101 72-73 0.3446247202568671 1101 74-75 0.1452221327092218 1101 76-77 0.06710241301231434 1101 78-79 0.32237082266601647 1101 80-81 -0.08541746339466272 1101 82-83 0.15015474939278306 1101 84-85 0.3582708903277947 1101 86-87 0.482682755855123 1101 88-89 0.696951813292614 1101 90-91 0.5672949341680038 1102 1 0.019547426085438246 1102 2 0.009405761210039998 1102 3 0.17626691559253516 1102 4 -0.11869933658330467 1102 5 -0.17898498162567478 1102 6 -0.01667763260378763 1102 7 -0.027016910862236898 1102 8 -0.09488709041357168 1102 9 0.010738454839810174 1102 10-11 0.06718760576908522 1102 12-13 -0.11665577117428683 1102 14-15 -0.07066117386201398 1102 16-17 -0.05445561856293324 1102 18-19 -0.13549029597843543 1102 20-21 -0.050108470915049 1102 22-23 -0.13464239702379643 1102 24-25 0.06850743701805584 1102 26-27 0.0345241447983895 1102 28-29 -0.06841522390079291 1102 30-31 0.0020996654351250754 1102 32-33 -0.026818182643438604 1102 34-35 -0.04439620245886999 1102 36-37 -0.07044331826910621 1102 38-39 0.11187380766615718 1102 40-41 -0.013527460180256412 1102 42-43 0.020353337498917767 1102 44-45 0.08670694346410102 1102 46-47 -0.06112549000617662 1102 48-49 -0.040997546537965945 1102 50-51 0.1251356032641695 1102 52-53 0.1310268372684149 1102 54-55 0.09443369565622817 1102 56-57 0.08293014599092174 1102 58-59 -0.09074971654581532 1102 60-61 0.02284374284690216 1102 62-63 -0.09195114150540462 1102 64-65 0.0014897729715031005 1102 66-67 -0.03065387652855378 1102 68-69 0.21301954888265584 1102 70-71 -0.07718993932375184 1102 72-73 -0.20669612341367127 1102 74-75 0.08567850661262355 1102 76-77 0.2762050437787309 1102 78-79 -0.19683127969567238 1102 80-81 -0.9813226061791411 1102 82-83 -0.6898452506072204 1102 84-85 -0.737267830210925 1102 86-87 -0.7291498559774894 1102 88-89 -1.8744767581359554 1102 90-91 -1.0327050658319976 1103 1 -0.0570581486262256 1103 2 0.022041515493064878 1103 3 0.0014596427946713675 1103 4 -0.2683903987809444 1103 5 -0.0010086597124043806 1103 6 -0.022263506312569348 1103 7 -0.12439520619205524 1103 8 -0.06735729059426632 1103 9 -0.1627083430698164 1103 10-11 -0.13111333742340747 1103 12-13 -0.2030976925660184 1103 14-15 -0.14941422825756234 1103 16-17 -0.05014690885445816 1103 18-19 -0.08708747893804514 1103 20-21 -0.04963853279966912 1103 22-23 -0.025822200523343497 1103 24-25 0.033713413077904875 1103 26-27 -0.08800433256166684 1103 28-29 -0.13451472945471465 1103 30-31 -0.00703703155508606 1103 32-33 0.07331219693763558 1103 34-35 0.023307127870474176 1103 36-37 5.875811829767486E-4 1103 38-39 -0.013791424822500176 1103 40-41 -0.11938033523517078 1103 42-43 0.02243450946194514 1103 44-45 0.04214591462040573 1103 46-47 0.043584646876652755 1103 48-49 -0.07574937213463784 1103 50-51 -0.06540324666770658 1103 52-53 -0.04135053481154927 1103 54-55 -0.15390460818824891 1103 56-57 -0.12769258366878233 1103 58-59 0.2842763931702237 1103 60-61 0.09021017250213959 1103 62-63 0.05251479222706479 1103 64-65 -0.06297822961415278 1103 66-67 0.06378101536122927 1103 68-69 -0.02236641965674835 1103 70-71 0.055799969129473936 1103 72-73 0.025799069340784797 1103 74-75 -8.153135768367292E-4 1103 76-77 -0.2875964781966118 1103 78-79 0.015587114943542701 1103 80-81 0.20792742028019262 1103 82-83 -0.2666448253015261 1103 84-85 0.14510191241305392 1103 86-87 0.6604605336329001 1103 88-89 -0.12150056765976558 1103 90-91 0.5672949341680038 1104 1 0.10501903190213113 1104 2 0.14285270515860304 1104 3 0.02856252766045486 1104 4 -0.18311115547548695 1104 5 0.08067615592046451 1104 6 0.047832655880348796 1104 7 0.11018299511518137 1104 8 -0.035322516596323794 1104 9 -0.09865808538343401 1104 10-11 -0.04133382566114108 1104 12-13 0.012711037585056317 1104 14-15 -0.04497649255409186 1104 16-17 0.014595388685705757 1104 18-19 -0.12387088412778269 1104 20-21 -0.04764037823927936 1104 22-23 -0.04402019626018472 1104 24-25 -0.13977639681609588 1104 26-27 -0.0716276635480142 1104 28-29 -0.05336843496241528 1104 30-31 -0.12515481175486798 1104 32-33 -0.060471846744235336 1104 34-35 0.008185105729097586 1104 36-37 -0.1441552979353844 1104 38-39 -0.031401359300787135 1104 40-41 -0.13749197386972867 1104 42-43 -0.08265498310137076 1104 44-45 -0.18467331616739813 1104 46-47 -0.040456654582072815 1104 48-49 0.040500609021052014 1104 50-51 -0.12271477708490153 1104 52-53 -0.09726405906193492 1104 54-55 0.14437491328465768 1104 56-57 0.012474110391444526 1104 58-59 -0.10273577007134804 1104 60-61 -0.2033053474080475 1104 62-63 0.0778530120977905 1104 64-65 -0.04343766646946534 1104 66-67 0.05843620839923602 1104 68-69 -0.18449040266705907 1104 70-71 -0.10884135650677251 1104 72-73 -0.30571322684617286 1104 74-75 -0.4697403425201756 1104 76-77 0.04198694457986818 1104 78-79 0.19891886231691558 1104 80-81 -0.0874794155283567 1104 82-83 -0.29701356294407333 1104 84-85 0.26888386943392106 1104 86-87 -0.35739660922423866 1104 88-89 -0.6833570670162601 1104 90-91 -0.7660383991653248 1201 1 -0.08232433454647747 1201 2 0.016051820942124095 1201 3 0.030014801818971648 1201 4 -0.04518366636754223 1201 5 0.07108857607751418 1201 6 -0.07553996168316957 1201 7 -0.04208088181422198 1201 8 -0.12064683640508633 1201 9 0.05260976280893459 1201 10-11 -0.04241660528817448 1201 12-13 -0.13348027704435594 1201 14-15 0.010923210565088937 1201 16-17 0.07043404488229044 1201 18-19 0.013041298267566503 1201 20-21 0.03130293166059772 1201 22-23 -0.012703124970222746 1201 24-25 -0.03247839786995854 1201 26-27 0.06584679313436936 1201 28-29 0.05900288455075042 1201 30-31 -0.015636632623923674 1201 32-33 0.08463508153150201 1201 34-35 0.014324234954962378 1201 36-37 0.016019644022449597 1201 38-39 0.09551886366172369 1201 40-41 0.14951108250348 1201 42-43 0.04747983406944911 1201 44-45 0.10415320746056977 1201 46-47 0.11009256974531922 1201 48-49 0.07204201270462818 1201 50-51 0.018287135790231446 1201 52-53 -0.03771218318967584 1201 54-55 0.1490224871537933 1201 56-57 0.1487865936700885 1201 58-59 0.24592721219305957 1201 60-61 0.22436127676515838 1201 62-63 0.024624404954671775 1201 64-65 0.11405324341058787 1201 66-67 0.06647711896614084 1201 68-69 0.26522485090805503 1201 70-71 0.07857562384307215 1201 72-73 0.1288527386203242 1201 74-75 0.009365779118056139 1201 76-77 0.31133140356987354 1201 78-79 -0.05085745883371828 1201 80-81 0.4114268665703378 1201 82-83 0.4945991938372245 1201 84-85 0.15625068830759403 1201 86-87 0.6604605336329001 1201 88-89 0.696951813292614 1201 90-91 0.5672949341680038 1202 1 0.07293750467311355 1202 2 0.1045409172112386 1202 3 -0.12962024370271763 1202 4 -0.08400238004617933 1202 5 -0.07044842372074811 1202 6 -0.026029018905795454 1202 7 -0.09726007264089986 1202 8 0.10687703075877408 1202 9 0.001933933233829066 1202 10-11 -0.0035718606196510905 1202 12-13 0.01696257517120614 1202 14-15 -0.03814845925783317 1202 16-17 0.020653030584774967 1202 18-19 0.08302236072241698 1202 20-21 -0.0729641983576883 1202 22-23 -0.016493887873458846 1202 24-25 -0.01063514347560357 1202 26-27 -0.04245188644366493 1202 28-29 -0.05311909666789205 1202 30-31 -0.008975953084927824 1202 32-33 -0.07262311831861723 1202 34-35 -0.14487810260946077 1202 36-37 -0.19786702476503848 1202 38-39 -0.3036144184823968 1202 40-41 -0.12241907733075408 1202 42-43 -0.23319981548178959 1202 44-45 -4.7061433500061867E-4 1202 46-47 -0.16469054392952387 1202 48-49 -0.19017429580389233 1202 50-51 -0.12817055229005803 1202 52-53 -0.1898284161968391 1202 54-55 -0.4840238156783059 1202 56-57 -0.09229137127181275 1202 58-59 -0.05900982865176019 1202 60-61 -0.23200618324362665 1202 62-63 -0.13141557501938905 1202 64-65 -0.08944066358200331 1202 66-67 0.10790410539271278 1202 68-69 -0.057343634656817244 1202 70-71 -0.0781951258605389 1202 72-73 -0.43439796050512314 1202 74-75 -0.1810177617877642 1202 76-77 0.09937888509364257 1202 78-79 0.11760820801968208 1202 80-81 0.19010582202348303 1202 82-83 -0.6812982420602083 1202 84-85 -0.4745557033683667 1202 86-87 -1.0270394663670999 1202 88-89 0.34912572633609074 1202 90-91 0.5672949341680038 1203 1 0.049127072103139824 1203 2 0.07665923782192152 1203 3 0.039228356806184195 1203 4 -0.11306090421920345 1203 5 -0.025931884280538497 1203 6 -0.031639705928697026 1203 7 0.05520812074332326 1203 8 0.10279306129312715 1203 9 -0.06519074870001162 1203 10-11 -0.028174277922452973 1203 12-13 -0.09020981668629702 1203 14-15 -0.06415990963951401 1203 16-17 -0.08278526458062885 1203 18-19 -0.11692992935390834 1203 20-21 -0.05343811693274603 1203 22-23 0.02616196454383868 1203 24-25 -0.02777890937890959 1203 26-27 -0.047343743950023054 1203 28-29 0.08453357508783199 1203 30-31 0.01943689678784466 1203 32-33 0.020465112740680524 1203 34-35 0.013235250870458515 1203 36-37 0.03402618006696656 1203 38-39 0.05171113035022046 1203 40-41 0.11562839401840108 1203 42-43 -0.034035812434972 1203 44-45 -0.0023972129076668125 1203 46-47 -0.013716211917198962 1203 48-49 0.05355853932824317 1203 50-51 0.09767619030405683 1203 52-53 0.30880505937296476 1203 54-55 -0.009853619754217391 1203 56-57 0.06438650567103821 1203 58-59 0.03337734820389926 1203 60-61 -0.11070250526456249 1203 62-63 0.06303815580135108 1203 64-65 0.27812629297573466 1203 66-67 -0.05788141653395229 1203 68-69 -0.23862618595484975 1203 70-71 0.27652763326858576 1203 72-73 -0.19068305151824205 1203 74-75 -0.005579435240406383 1203 76-77 -0.049378160507188795 1203 78-79 0.18219917261064467 1203 80-81 -0.3946119526270664 1203 82-83 0.10219609306203381 1203 84-85 0.47761820967511426 1203 86-87 0.5004605336329035 1203 88-89 -1.0363815200407203 1203 90-91 0.5672949341680038 1204 1 -0.02780488794680025 1204 2 -0.14858732896959026 1204 3 -0.204688621970341 1204 4 -0.2242469216973575 1204 5 -0.21471465581612392 1204 6 -0.19557853239560785 1204 7 -0.07393045603570414 1204 8 -0.12631430449962977 1204 9 -0.08434180987354978 1204 10-11 -0.013668972054780681 1204 12-13 -0.07926256325309566 1204 14-15 -0.16318300576935485 1204 16-17 -0.13943788630347598 1204 18-19 -0.1486012776685257 1204 20-21 -0.002756156882810501 1204 22-23 -0.08187018277201474 1204 24-25 0.01034531034643038 1204 26-27 -0.04661652797000215 1204 28-29 -0.05099389058008086 1204 30-31 0.014929354880123924 1204 32-33 -0.11099280405332479 1204 34-35 -0.0630238303552133 1204 36-37 0.06222515178905752 1204 38-39 -0.10476983714958976 1204 40-41 -0.11871602837155137 1204 42-43 -0.08944620250183277 1204 44-45 -0.12358189054637592 1204 46-47 -0.19921467387567304 1204 48-49 -0.2357962777189755 1204 50-51 -0.24597422702427707 1204 52-53 -0.037215137515154595 1204 54-55 -0.10680828963688072 1204 56-57 -0.21255407034436047 1204 58-59 -0.20974637036631094 1204 60-61 -0.403883340298691 1204 62-63 -0.057723460717305386 1204 64-65 0.1041234328965075 1204 66-67 -0.12092315366362527 1204 68-69 -0.12895025816746397 1204 70-71 -0.025424085202175206 1204 72-73 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2401 40-41 -0.11367717867688043 2401 42-43 0.02165712108121909 2401 44-45 0.014707600534059395 2401 46-47 -0.14660082479880288 2401 48-49 -0.059281832831402426 2401 50-51 -0.13535536966750783 2401 52-53 -0.1126666063236641 2401 54-55 -0.14454225710498037 2401 56-57 0.04090008367317921 2401 58-59 0.05457863539145791 2401 60-61 0.040801032650350066 2401 62-63 0.0035768465113221737 2401 64-65 -0.10282934549235989 2401 66-67 0.12365543179917182 2401 68-69 -0.0415332775519488 2401 70-71 -0.20229674142304788 2401 72-73 0.014241989346061246 2401 74-75 -6.242673876215576E-4 2401 76-77 0.11511009554732965 2401 78-79 0.0733891623961398 2401 80-81 0.3315494897490794 2401 82-83 0.13572473496276416 2401 84-85 0.1245046565615624 2401 86-87 -0.45960807700174655 2401 88-89 0.696951813292614 2401 90-91 0.14624230258905868 2402 1 0.01206691032356133 2402 2 0.1344423520787501 2402 3 -0.005612391424349994 2402 4 0.09286427272720488 2402 5 -0.059940984381654516 2402 6 -0.018024943542442884 2402 7 0.042912873018352116 2402 8 0.11863229284014665 2402 9 0.12564848284700503 2402 10-11 0.10422587374590364 2402 12-13 0.0941114674390775 2402 14-15 0.04705596498131115 2402 16-17 0.029870195480036443 2402 18-19 0.014825631818986551 2402 20-21 0.002872550408000052 2402 22-23 0.012128850287020043 2402 24-25 0.05774940204677392 2402 26-27 0.021506104381622038 2402 28-29 0.06495066305547681 2402 30-31 0.04672582499816258 2402 32-33 0.01175777993785232 2402 34-35 0.08636633070735655 2402 36-37 0.11121846799170498 2402 38-39 -0.24087651889096406 2402 40-41 0.014771832493771342 2402 42-43 0.11433780936278026 2402 44-45 -0.05786579124267632 2402 46-47 0.20061129041827996 2402 48-49 0.05036352627677587 2402 50-51 0.037611450480596886 2402 52-53 0.11335525223311294 2402 54-55 0.19515586509134408 2402 56-57 -0.05635596272939836 2402 58-59 -0.10345443996461512 2402 60-61 0.16973948613075152 2402 62-63 0.06510356336224277 2402 64-65 0.03250352256122113 2402 66-67 0.07445895113954037 2402 68-69 0.16089068914894966 2402 70-71 0.26108684113226843 2402 72-73 0.2505200243165362 2402 74-75 0.03886855743671447 2402 76-77 -0.04791471033461647 2402 78-79 -0.0540995286880559 2402 80-81 -0.07212229410112769 2402 82-83 0.12333482602113577 2402 84-85 0.08402846608537118 2402 86-87 0.5004605336329035 2402 88-89 -0.04524851511460781 2402 90-91 0.5672949341680038 2403 1 -0.02601500167552473 2403 2 0.1237504641429581 2403 3 0.17518797794277674 2403 4 0.1529610299782007 2403 5 0.10901826527080516 2403 6 0.08107714352294693 2403 7 0.1286677418799158 2403 8 -0.026364466124618957 2403 9 0.08633467502880166 2403 10-11 0.13793364829889043 2403 12-13 0.08133180843832832 2403 14-15 0.1527368999317673 2403 16-17 0.03597221298667108 2403 18-19 0.13545666087539843 2403 20-21 0.1410172669157035 2403 22-23 -0.009083548915782558 2403 24-25 0.0730311604117233 2403 26-27 0.041636814374996334 2403 28-29 0.13421203969401319 2403 30-31 0.10350593965922883 2403 32-33 0.07333120355812639 2403 34-35 0.07802335141420969 2403 36-37 0.07288084601465528 2403 38-39 0.1273678336830102 2403 40-41 0.09723745417691987 2403 42-43 0.08854123291941107 2403 44-45 0.12090915330061591 2403 46-47 0.06557280561411005 2403 48-49 0.06964697651362428 2403 50-51 0.03883848688285241 2403 52-53 0.019550035348999018 2403 54-55 0.04085644690410817 2403 56-57 0.027673114493829587 2403 58-59 0.062131440402957594 2403 60-61 0.11973567757763703 2403 62-63 0.1658089413268442 2403 64-65 0.06297152844188503 2403 66-67 0.19385664186685148 2403 68-69 0.25010428647127014 2403 70-71 0.192952901866434 2403 72-73 0.3324640689651659 2403 74-75 0.020557505523250086 2403 76-77 0.08907771778246598 2403 78-79 0.03238353731755694 2403 80-81 0.13924715973615065 2403 82-83 0.2162433724434294 2403 84-85 0.15625068830759403 2403 86-87 0.2883675103770855 2403 88-89 0.0012996393795674521 2403 90-91 0.5672949341680038 2404 1 -0.0039013036307196103 2404 2 0.03282851375147544 2404 3 -0.029073991530410126 2404 4 0.014806023373751032 2404 5 -0.00414127762692118 2404 6 -0.058968473051983494 2404 7 -0.03254139001988676 2404 8 -0.00907582324698808 2404 9 0.025337627033842125 2404 10-11 0.03773814869203562 2404 12-13 0.04644624984990742 2404 14-15 -0.02158163175301553 2404 16-17 0.05509878630000031 2404 18-19 0.010406176321232863 2404 20-21 0.10054182435883519 2404 22-23 0.025680066305092453 2404 24-25 0.0077641434586652736 2404 26-27 0.03222083530633313 2404 28-29 -0.028739419041322378 2404 30-31 -0.05563893491469685 2404 32-33 0.04535759496886271 2404 34-35 0.04354684819248433 2404 36-37 -0.0067220973844257514 2404 38-39 -0.015906184940909895 2404 40-41 0.06060824540092824 2404 42-43 0.02109921595874198 2404 44-45 0.037783016108207335 2404 46-47 0.021769271795989198 2404 48-49 0.12379497437357401 2404 50-51 0.11855903471492013 2404 52-53 -0.025788746653709893 2404 54-55 0.01134881847153224 2404 56-57 0.13571813221120976 2404 58-59 -0.07049925564907511 2404 60-61 -0.01840784710670107 2404 62-63 0.13784627517424752 2404 64-65 0.10205553257142697 2404 66-67 0.10300342823651931 2404 68-69 -0.03400161948817981 2404 70-71 -0.05357974990446479 2404 72-73 0.0415885944693386 2404 74-75 0.05933373791367558 2404 76-77 0.16214522280757393 2404 78-79 0.09706041512089314 2404 80-81 -0.17303306374325444 2404 82-83 0.2017219679703146 2404 84-85 -0.014441743254408834 2404 86-87 0.029125910808915023 2404 88-89 -0.21045559411479076 2404 90-91 0.5672949341680038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 345.0 32 2139.0 33 4148.0 34 5457.0 35 6871.0 36 9944.0 37 14452.0 38 37531.0 39 228828.0 40 251480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.57799322534029 10.473281789850127 33.691548068544876 30.257176916264704 2 27.26665193464686 17.00924553049528 29.38592426791366 26.3381782669442 3 27.763809917517364 19.383865903789854 21.324319460308015 31.52800471838477 4 26.304816922037954 20.384206240455875 20.63295492410136 32.678021913404805 5 27.271123592102054 20.458331922688338 20.74877630258959 31.521768182620026 6 28.3129814382877 22.88968993726045 23.5017613758867 25.295567248565153 7 26.127699306319034 22.368850107535696 23.331058482668666 28.1723921034766 8 25.155334287226328 23.60172413485885 23.846730897045486 27.396210680869338 9 25.478743213312686 23.772783401549155 23.927271301778838 26.821202083359317 10-11 24.90542738979596 22.42711717025208 24.06884066363868 28.59861477631328 12-13 25.614699692984257 22.25632518366598 23.76512137189532 28.36385375145445 14-15 25.92572464090918 21.97425558036311 23.573036070340997 28.526983708386716 16-17 25.74816155834992 21.639264516427925 23.449463568830865 29.163110356391293 18-19 26.01401616866444 21.962673442514287 23.352084517532685 28.67122587128859 20-21 26.191222877751425 21.980313929391723 23.18298530493987 28.645477887916982 22-23 25.71813709331105 21.59480692576206 23.567066814680448 29.11998916624644 24-25 26.018381743699763 21.961069761889064 23.483229955328586 28.53731853908259 26-27 26.122710077707232 21.991094226927935 23.51726362193051 28.368932073434316 28-29 25.875475981318903 21.639175423059857 23.676651657403927 28.808696938217317 30-31 26.25325419325409 21.868267762415826 23.652572438653667 28.225905605676417 32-33 26.284697391740114 21.837523753436134 23.707895376973205 28.16988347785055 34-35 25.88451258833712 21.528672113528064 24.019232049611457 28.567583248523356 36-37 26.169793284741306 21.799199426454773 23.937248576094316 28.093758712709604 38-39 26.310525300477238 21.790471118119218 23.934595351724024 27.96440822967952 40-41 25.954861784581006 21.49900637405777 24.05616618878782 28.489965652573396 42-43 26.181479610862247 21.839317828871906 24.157788655341204 27.82141390492464 44-45 26.23519060693861 21.731693997360477 24.05581428275259 27.97730111294833 46-47 25.96419323190305 21.602718233638004 24.210458994487603 28.222629539971344 48-49 26.17952287307304 21.806718665989056 24.183367582178818 27.830390878759086 50-51 26.33391092285971 21.764054678475166 24.115164933898086 27.78686946476704 52-53 26.085514190932546 21.52458181945834 24.395415372193845 27.99448861741527 54-55 26.437674672109285 21.7853567273163 24.280921070433205 27.496047530141205 56-57 26.49656190180834 21.813690367978097 24.193945097368225 27.495802632845333 58-59 26.226645380942298 21.503974419063887 24.400387330394256 27.868992869599563 60-61 26.48735953935027 21.8542535329695 24.256220447717904 27.402166479962318 62-63 26.60997409908224 21.771742547871785 24.10522060694983 27.513062746096146 64-65 26.326789300727583 21.564382881979725 24.301390252937 27.8074375643557 66-67 26.58678441518022 21.74444163473322 24.291855511680836 27.376918438405728 68-69 26.822766150964867 21.732955181884574 23.979013490687347 27.46526517646321 70-71 26.366907222927022 21.530478547511596 24.386477549237775 27.716136680323604 72-73 26.98372920308546 21.694803369069593 24.137256579832037 27.184210848012906 74-75 26.904867208514894 21.717190026319035 23.851714362361605 27.526228402804463 76-77 26.337606912353735 21.651828660381646 24.02882479492068 27.98173963234394 78-79 26.837742485496445 21.6389821238995 24.06785840670005 27.455416983904 80-81 27.093270811437197 21.583528714258765 23.82973562939607 27.493464844907965 82-83 26.98384769680121 21.233768518844354 23.929338072280675 27.853045712073758 84-85 27.681429458697625 20.995174621374993 23.508193588457313 27.81520233147007 86-87 28.305842885099246 20.66326530612245 22.93821638244339 28.092675426334917 88-89 27.771628313428486 19.003786775415087 24.043110981648706 29.181473929507717 90-91 23.671144859813083 19.4071261682243 27.14661214953271 29.775116822429908 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.5 10 2.5 11 4.5 12 7.0 13 7.0 14 9.0 15 21.0 16 37.0 17 51.0 18 92.0 19 162.0 20 227.0 21 311.0 22 414.0 23 529.0 24 716.0 25 934.5 26 1187.0 27 1553.0 28 2008.0 29 2487.5 30 3002.5 31 3673.5 32 4388.0 33 5054.0 34 6002.0 35 7170.5 36 8447.5 37 9740.5 38 11118.0 39 12761.0 40 14403.0 41 16212.5 42 18263.0 43 20300.5 44 22494.5 45 24935.0 46 27424.5 47 29931.0 48 32227.0 49 34437.0 50 36651.0 51 38644.5 52 40153.0 53 41205.0 54 41856.5 55 42055.5 56 42163.5 57 41945.5 58 41215.5 59 40268.0 60 39243.5 61 37944.5 62 36297.5 63 34185.5 64 31700.5 65 29380.5 66 26993.0 67 24607.5 68 22008.0 69 19084.5 70 16370.0 71 13888.5 72 11588.0 73 9447.0 74 7542.5 75 6058.5 76 4807.5 77 3616.5 78 2719.0 79 2029.0 80 1462.0 81 1079.0 82 815.0 83 586.5 84 406.5 85 274.0 86 193.5 87 138.5 88 102.5 89 70.5 90 50.0 91 34.5 92 21.5 93 16.0 94 14.5 95 11.5 96 6.0 97 4.0 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013007631737908693 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05511650959509275 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 5.199315770044662E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.014600671630895021 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9452.0 32-33 20504.0 34-35 23133.0 36-37 24498.0 38-39 25767.0 40-41 27172.0 42-43 28096.0 44-45 28109.0 46-47 28139.0 48-49 27747.0 50-51 27171.0 52-53 26226.0 54-55 25273.0 56-57 24032.0 58-59 22670.0 60-61 21092.0 62-63 20179.0 64-65 19069.0 66-67 17255.0 68-69 15652.0 70-71 14832.0 72-73 13039.0 74-75 12195.0 76-77 10777.0 78-79 9772.0 80-81 8912.0 82-83 7778.0 84-85 6787.0 86-87 5961.0 88-89 5334.0 90-91 4586.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.91445117417507 #Duplication Level Percentage of deduplicated Percentage of total 1 99.91935063711516 99.8338708058732 2 0.07690298573442958 0.15367439226621893 3 0.0028053155179932994 0.008408746810520912 4 6.55788795774741E-4 0.002620911104640257 5 2.8527283664424335E-4 0.0014251439454104832 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016036806252216198 0.0 0.0 0.0 0.0 2 0.0016036806252216198 0.0 0.0 0.0 0.0 3 0.0016036806252216198 0.0 0.0 0.0 0.0 4 0.0016036806252216198 0.0 0.0 0.0 0.0 5 0.0017818673613573552 0.0 0.0 0.0 0.0 6 0.0017818673613573552 0.0 0.0 0.0 1.7818673613573553E-4 7 0.0017818673613573552 0.0 0.0 0.0 1.7818673613573553E-4 8 0.007305656181565157 0.0 0.0 0.0 1.7818673613573553E-4 9 0.008018403126108098 0.0 0.0 0.0 3.5637347227147106E-4 10-11 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 5.345602084072065E-4 12-13 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 8.018403126108099E-4 14-15 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0011582137848822809 16-17 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0013364005210180164 18-19 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 20-21 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 22-23 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 24-25 0.008018403126108098 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 26-27 0.008463869966447438 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 28-29 0.008820243438718909 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 30-31 0.009176616910990379 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 32-33 0.009622083751329719 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 34-35 0.009800270487465453 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 36-37 0.010423924063940528 1.7818673613573553E-4 0.0 0.0 0.0016036806252216198 38-39 0.010958484272347736 1.7818673613573553E-4 0.0 0.0 0.0017818673613573552 40-41 0.01158213784882281 1.7818673613573553E-4 0.0 8.909336806786777E-5 0.0017818673613573552 42-43 0.01158213784882281 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 44-45 0.01193851132109428 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 46-47 0.012116698057230016 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 48-49 0.012116698057230016 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 50-51 0.012294884793365752 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 52-53 0.012294884793365752 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.0017818673613573552 54-55 0.01238397816143362 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.001960054097493091 56-57 0.012651258265637223 1.7818673613573553E-4 0.0 1.7818673613573553E-4 0.001960054097493091 58-59 0.01274035163370509 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