##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-21A_S1_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056620
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.33267967670496	40.0	40.0	40.0	40.0	40.0
2	39.22381177717628	40.0	40.0	40.0	40.0	40.0
3	39.18138214305995	40.0	40.0	40.0	40.0	40.0
4	39.045223448354186	40.0	40.0	40.0	40.0	40.0
5	39.07691696163238	40.0	40.0	40.0	40.0	40.0
6	39.19407923378319	40.0	40.0	40.0	40.0	40.0
7	39.19761882228237	40.0	40.0	40.0	40.0	40.0
8	39.19304196399841	40.0	40.0	40.0	40.0	40.0
9	39.22323635744166	40.0	40.0	40.0	40.0	40.0
10	39.233593912664915	40.0	40.0	40.0	40.0	40.0
11	39.20878272226533	40.0	40.0	40.0	40.0	40.0
12	39.21937120251368	40.0	40.0	40.0	40.0	40.0
13	39.12814446063864	40.0	40.0	40.0	40.0	40.0
14	39.24237663493025	40.0	40.0	40.0	40.0	40.0
15	39.201658117393194	40.0	40.0	40.0	40.0	40.0
16	39.20420964963753	40.0	40.0	40.0	40.0	40.0
17	39.2465030001325	40.0	40.0	40.0	40.0	40.0
18	39.19289810906476	40.0	40.0	40.0	40.0	40.0
19	39.25371846075221	40.0	40.0	40.0	40.0	40.0
20	38.812254168953835	40.0	40.0	40.0	40.0	40.0
21	39.068493876701176	40.0	40.0	40.0	40.0	40.0
22	39.06564327762109	40.0	40.0	40.0	40.0	40.0
23	39.20431943366584	40.0	40.0	40.0	40.0	40.0
24	39.23016410819405	40.0	40.0	40.0	40.0	40.0
25	38.91416024682478	40.0	40.0	40.0	40.0	40.0
26	39.167840851015505	40.0	40.0	40.0	40.0	40.0
27	39.18360810887547	40.0	40.0	40.0	40.0	40.0
28	38.823633851337284	40.0	40.0	40.0	40.0	40.0
29	39.106320152940505	40.0	40.0	40.0	40.0	40.0
30	38.642467490677824	40.0	40.0	40.0	40.0	40.0
31	38.866373909257824	40.0	40.0	40.0	40.0	40.0
32	38.97182714694024	40.0	40.0	40.0	40.0	40.0
33	38.96307281709602	40.0	40.0	40.0	40.0	40.0
34	38.99944729420227	40.0	40.0	40.0	40.0	40.0
35	38.707452064129015	40.0	40.0	40.0	40.0	40.0
36	38.74556226457951	40.0	40.0	40.0	40.0	40.0
37	38.84810433268346	40.0	40.0	40.0	40.0	40.0
38	38.548734644432244	40.0	40.0	40.0	40.0	40.0
39	38.662599610077415	40.0	40.0	40.0	40.0	40.0
40	38.33889193844523	40.0	40.0	40.0	40.0	40.0
41	38.38678427438436	40.0	40.0	40.0	40.0	40.0
42	38.09591338418731	40.0	40.0	40.0	40.0	40.0
43	38.41117904260756	40.0	40.0	40.0	40.0	40.0
44	37.88119475308058	40.0	40.0	40.0	24.0	40.0
45	38.169578467187826	40.0	40.0	40.0	40.0	40.0
46	37.76734114440385	40.0	40.0	40.0	24.0	40.0
47	37.13021142889591	40.0	40.0	40.0	24.0	40.0
48	37.47719331453124	40.0	40.0	40.0	24.0	40.0
49	37.05862845677727	40.0	40.0	40.0	24.0	40.0
50	37.27110219378774	40.0	40.0	40.0	24.0	40.0
51	36.78514129961576	40.0	40.0	40.0	24.0	40.0
52	36.781067933599594	40.0	40.0	40.0	24.0	40.0
53	35.97744506066514	40.0	40.0	40.0	24.0	40.0
54	35.18557286441673	40.0	40.0	40.0	12.0	40.0
55	35.09860498570915	40.0	40.0	40.0	12.0	40.0
56	34.82187352122806	40.0	40.0	40.0	12.0	40.0
57	34.8657909182109	40.0	40.0	40.0	12.0	40.0
58	34.770805019780056	40.0	40.0	40.0	12.0	40.0
59	34.30051674206432	40.0	24.0	40.0	12.0	40.0
60	33.53855880070413	40.0	24.0	40.0	12.0	40.0
61	33.41526376559217	40.0	24.0	40.0	12.0	40.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.017589905330552824
1101	2	0.033306186578009545
1101	3	0.02324734843950438
1101	4	-0.009761197549238432
1101	5	0.05615008459744075
1101	6	0.05115869413659624
1101	7	0.04692390358693643
1101	8	0.0806160480865259
1101	9	-0.009197621426920932
1101	10	-0.061666188233246544
1101	11	0.09531229643339856
1101	12	0.03184787468393324
1101	13	0.028282575502920793
1101	14	0.02959091695866789
1101	15	0.08557447927952211
1101	16	0.04407233150845258
1101	17	0.044432445856500635
1101	18	0.11556201546253675
1101	19	0.140544532519705
1101	20	0.058011403360247016
1101	21	0.0872000109333726
1101	22	0.07625423658293329
1101	23	0.06967078114926295
1101	24	0.13275483769824348
1101	25	0.08542732902293437
1101	26	0.1048917340068698
1101	27	0.16657461308812316
1101	28	-0.010859531295665192
1101	29	0.08228300707079228
1101	30	-0.00555267452473629
1101	31	0.02065875383519966
1101	32	0.09309927255957007
1101	33	0.05553425757091901
1101	34	0.04384119246531526
1101	35	0.054330383243033964
1101	36	-0.018929924826181832
1101	37	0.005425757870668235
1101	38	-0.006883600305862103
1101	39	-0.046013625236327016
1101	40	0.13093519752604976
1101	41	0.08601589075971816
1101	42	-0.06117190308174969
1101	43	-0.19686260181124737
1101	44	-0.1425092276608737
1101	45	0.08847197374247884
1101	46	-0.014422875821487935
1101	47	-0.10382774000616735
1101	48	0.04329451404259288
1101	49	-0.044950637848202746
1101	50	-0.13978562729377586
1101	51	0.08019118765155042
1101	52	0.08696085405404119
1101	53	-0.1667267717948988
1101	54	0.0437468265413159
1101	55	-0.1066052937530344
1101	56	0.1596524303946225
1101	57	0.05505764544271585
1101	58	0.45799794829769525
1101	59	0.17943841019848605
1101	60	-0.21449339609418416
1101	61	-0.08778887808630031
1102	1	-0.033458198523781846
1102	2	-0.09653956843006029
1102	3	-0.03209601609445656
1102	4	-0.06478358903101622
1102	5	-0.1291637599286446
1102	6	-0.0990926319912262
1102	7	-0.05803252282382232
1102	8	-0.03229459086652753
1102	9	-0.1324502164729111
1102	10	-0.048120138905929366
1102	11	-0.0711455263510743
1102	12	-0.13763012483302361
1102	13	-0.1661974983596366
1102	14	-0.10852368169553728
1102	15	-0.1610783372766278
1102	16	0.013201136303109706
1102	17	-0.10761365165200942
1102	18	-0.1367700158335694
1102	19	-0.10083284000757686
1102	20	-0.17770084764561744
1102	21	-0.0677968542063283
1102	22	-0.1437775471481686
1102	23	-0.07664642302395208
1102	24	-0.11034789710843285
1102	25	-0.0682849392471141
1102	26	-0.1227496830733017
1102	27	-0.16263416367512207
1102	28	-0.20174054947433007
1102	29	-0.03288289917865228
1102	30	-0.10552292021709775
1102	31	-0.01024943606880413
1102	32	-0.04919149907761522
1102	33	-0.12069298430341036
1102	34	-0.13072692525675222
1102	35	-0.17199719353902765
1102	36	-0.13680208136519667
1102	37	-0.0834108451774469
1102	38	-0.1957119412046069
1102	39	-0.28883942829874343
1102	40	-0.06718867798925743
1102	41	-0.13945446600482825
1102	42	-0.08334899177160793
1102	43	-0.23219606233588053
1102	44	-0.25758810828585155
1102	45	-0.13410116962131724
1102	46	-0.16297455979374575
1102	47	-0.35911406223809905
1102	48	0.037425745513317565
1102	49	-0.0029324114410016477
1102	50	-0.1303988390871922
1102	51	-0.17128714693167524
1102	52	-0.023387285438523975
1102	53	-0.15235838316593941
1102	54	-0.1226415871418638
1102	55	0.19755912900899375
1102	56	-0.20837787848116562
1102	57	0.00890658269953093
1102	58	-0.09982150665059919
1102	59	0.0343783211187727
1102	60	-0.20604345458985307
1102	61	-0.3302193663687589
1103	1	0.03383328583799283
1103	2	-0.18137590836242623
1103	3	-0.10426564097009106
1103	4	-0.09693931552966006
1103	5	0.0028771485415504117
1103	6	-0.20820227292696813
1103	7	0.011048808165462276
1103	8	0.037614878114588635
1103	9	0.014970648632512962
1103	10	-0.060906716040427966
1103	11	-0.10768342447287438
1103	12	-0.001680517521926106
1103	13	-0.09680051884884477
1103	14	0.020714000762403373
1103	15	0.0460767257625605
1103	16	-0.044722827904884355
1103	17	0.015492083748405605
1103	18	-0.02160400070458479
1103	19	-0.03801878422812166
1103	20	0.021836470674145403
1103	21	0.04796847131148496
1103	22	0.025341869049697152
1103	23	0.021954020254838724
1103	24	-0.03983911773848803
1103	25	-0.01222498852413878
1103	26	-0.09101118908582606
1103	27	-0.12715463715493058
1103	28	0.00363872700512502
1103	29	-0.14636838470706692
1103	30	-0.07534439074904498
1103	31	-0.1520104208921893
1103	32	-0.03877158285101956
1103	33	-0.06567383009733874
1103	34	-0.0997909096374201
1103	35	0.09130534414503444
1103	36	-0.06540435421030111
1103	37	0.021028041244093743
1103	38	-0.047005985904732484
1103	39	-0.028672285728077895
1103	40	-0.018411490871017122
1103	41	0.1412899372533829
1103	42	-0.03901548907304431
1103	43	-0.10806640249988675
1103	44	0.09326893193279773
1103	45	0.1489663083346926
1103	46	0.034351461367009506
1103	47	-0.04280999007339403
1103	48	0.0678998879063002
1103	49	0.06905372955019828
1103	50	0.0762004997732717
1103	51	0.2732981715807057
1103	52	0.25065362900719634
1103	53	-0.0675357517996531
1103	54	0.21612344329027167
1103	55	0.28265522869346427
1103	56	0.44782446557818645
1103	57	0.5292071236782121
1103	58	-0.007198701185522793
1103	59	0.20760890113347585
1103	60	0.5526515110095573
1103	61	0.3453088923463312
1104	1	0.023233306776056395
1104	2	-0.007802503706095365
1104	3	-0.043526610388433085
1104	4	-0.04785684134203194
1104	5	-0.1420356458221761
1104	6	-0.01225762265303132
1104	7	-0.00471105509449643
1104	8	-0.0392692622035824
1104	9	-0.07014947398297267
1104	10	-0.04373094241194764
1104	11	-0.046956832342480936
1104	12	-0.1032259605398238
1104	13	-0.1558935277136584
1104	14	-0.14080032539659015
1104	15	-0.03199494113847834
1104	16	-0.07806029587155194
1104	17	-0.03909751473492662
1104	18	-0.03850370981758289
1104	19	-0.12547457158036224
1104	20	-0.10875344706277446
1104	21	-0.06525633192777036
1104	22	0.049642802175398515
1104	23	-0.005576418535717664
1104	24	-0.0038826647563467986
1104	25	0.1262149740084837
1104	26	-0.038502958113461716
1104	27	-0.05466161564435623
1104	28	-0.1434096803375695
1104	29	-0.09141254293660239
1104	30	-0.07646108726223844
1104	31	-0.02633472794088476
1104	32	0.10905768282082562
1104	33	-0.052960005346129435
1104	34	-0.004830244511971671
1104	35	-0.09096668409301856
1104	36	0.061466121087107695
1104	37	-0.02969112582192679
1104	38	0.04125760975438908
1104	39	-0.013377136827720904
1104	40	-0.029875942295582547
1104	41	-0.09833129808522045
1104	42	-0.136050956971296
1104	43	-0.06547472679518762
1104	44	-0.03657882487889452
1104	45	0.001190597105775737
1104	46	-0.14468518749309567
1104	47	0.019656948695910614
1104	48	-0.3459346413035007
1104	49	0.1420726211390786
1104	50	-0.15299593135865308
1104	51	0.10194226637413806
1104	52	0.20152865677776077
1104	53	0.08058650850188798
1104	54	0.20843475284843294
1104	55	0.16097055103420388
1104	56	0.2334187350454826
1104	57	0.12785458030861463
1104	58	-0.16007901460685758
1104	59	-0.17419385197950277
1104	60	-0.1566328599727811
1104	61	-0.20622965769649682
1201	1	-0.10952571814961942
1201	2	-0.14484203159612719
1201	3	-0.0076627664098580794
1201	4	0.00409621260793358
1201	5	0.007807190959027821
1201	6	-0.12228799691141745
1201	7	-0.0037602605519779786
1201	8	-0.09378451995021209
1201	9	-0.08166449657178987
1201	10	-0.07148271809873563
1201	11	-0.11585912199451798
1201	12	-0.09157881500868115
1201	13	-0.1223174783438381
1201	14	-0.11984184178947288
1201	15	-0.1149446681836892
1201	16	-0.03049919076301677
1201	17	-0.06289506453774152
1201	18	-0.06203771465320784
1201	19	-0.12415299169837368
1201	20	-0.0030817280661992186
1201	21	0.023714276149675584
1201	22	-0.05444519380694146
1201	23	-0.04263953229847317
1201	24	-0.12046311273373078
1201	25	-0.12078273364094372
1201	26	0.00925475434426204
1201	27	-0.03418115774899633
1201	28	-0.021161613730782847
1201	29	-0.05842175965081964
1201	30	0.03942440104768963
1201	31	-0.025795371950863455
1201	32	-0.07437346938585421
1201	33	-0.1099463476188447
1201	34	0.019523833758782416
1201	35	0.07915614818829653
1201	36	-0.10413789629144077
1201	37	-0.06893058831635557
1201	38	-0.046763956074201474
1201	39	-0.08266423016855384
1201	40	-0.16025465150700313
1201	41	-0.10722453889277972
1201	42	-0.06378741761145079
1201	43	0.030402721991400483
1201	44	0.1278732346444187
1201	45	-0.005982592240421525
1201	46	0.127119741568805
1201	47	0.659188413406639
1201	48	0.16664244647554938
1201	49	0.10218796411726316
1201	50	0.20711443510553096
1201	51	0.0241863377513738
1201	52	0.10616883553975498
1201	53	-0.045862857395604806
1201	54	0.16684125904471614
1201	55	0.03196458867977725
1201	56	0.35041728029128194
1201	57	0.30351816515800323
1201	58	0.16055357742448706
1201	59	0.3312310739773281
1201	60	0.0881313741715033
1201	61	0.2686597988576622
1202	1	-0.38441422081150023
1202	2	-0.2684916324611919
1202	3	-0.16301849299875215
1202	4	-0.08049506138655005
1202	5	-0.18316615471880482
1202	6	-0.09738241992175745
1202	7	-0.07096586947525196
1202	8	-0.10093420242924367
1202	9	-0.1176562126441354
1202	10	-0.11000099518874862
1202	11	-0.08505905635174571
1202	12	-0.10372536433757773
1202	13	-0.12025853855543289
1202	14	-0.05882641283797341
1202	15	-0.10658585081184668
1202	16	-0.16105587361975182
1202	17	-0.17484253203916467
1202	18	-0.08485059874318068
1202	19	-0.09523501360467179
1202	20	-0.12118105169427906
1202	21	-0.1037814214273709
1202	22	-0.1465574345046008
1202	23	-0.07297584425597847
1202	24	-0.11127699254048196
1202	25	-0.20811820590191132
1202	26	-0.0566172550883266
1202	27	-0.052700364807542144
1202	28	-0.05565764505929849
1202	29	-0.0815837774856476
1202	30	0.008594098242724613
1202	31	-0.07365306461454679
1202	32	-0.10141885299304221
1202	33	-0.09418575421805286
1202	34	-0.05518621690692527
1202	35	0.015426758132505825
1202	36	0.09070756412165082
1202	37	-0.1065695265384079
1202	38	0.020231047498441512
1202	39	-0.08373835261622276
1202	40	-0.20151396250931697
1202	41	0.08365809529847468
1202	42	-0.05527741442863743
1202	43	-0.03889030590791975
1202	44	0.2076233983338227
1202	45	-0.03177559478095304
1202	46	0.015049567444059164
1202	47	-0.2441165699501724
1202	48	0.05744128681006089
1202	49	-0.06145520607690713
1202	50	-0.22820295709041716
1202	51	0.23580568809363456
1202	52	0.08106636540092893
1202	53	-0.014648964544676346
1202	54	0.2594851112451124
1202	55	-0.11014198694490318
1202	56	0.34473937410225375
1202	57	0.3250252257551196
1202	58	0.17977808387093575
1202	59	-0.07874019280770739
1202	60	0.12149245713304424
1202	61	0.08433019058448821
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2403	7	0.11055018900513147
2403	8	0.10859895534254349
2403	9	0.08283278802360883
2403	10	0.21939634381610063
2403	11	0.032794705949768854
2403	12	0.020299332860574282
2403	13	0.05381311358279817
2403	14	0.06711558455706523
2403	15	0.08690287622868453
2403	16	0.08537267280134841
2403	17	0.12406751499034385
2403	18	0.07468907461193908
2403	19	0.1252970672089333
2403	20	0.16340726119521776
2403	21	0.0906257618827695
2403	22	0.1126338347410396
2403	23	0.10527996727606137
2403	24	0.17621327841593626
2403	25	0.11325673586478047
2403	26	0.10583310621509412
2403	27	0.06675678570643129
2403	28	0.10241940942052707
2403	29	0.15609488158274587
2403	30	0.20236800140815348
2403	31	0.19273817429475315
2403	32	0.20829915092322437
2403	33	0.13586298852898437
2403	34	0.08622403904865195
2403	35	0.2045047126085393
2403	36	0.05732158778490515
2403	37	0.07324311383877102
2403	38	0.045158496273359106
2403	39	0.05501074450034338
2403	40	0.0885512597075433
2403	41	0.15767475827767186
2403	42	0.1561297578782117
2403	43	0.1431801000397499
2403	44	0.09453215006718096
2403	45	0.17946752850506442
2403	46	-0.0476684465661279
2403	47	-0.018331283019435318
2403	48	-0.00791642508075796
2403	49	-0.06927899851379493
2403	50	-0.05364170410509672
2403	51	-0.06494309020038713
2403	52	0.001288708204640443
2403	53	0.07467263546434566
2403	54	0.07990708642988409
2403	55	-0.197202547677918
2403	56	-0.11353138371419647
2403	57	-0.48893036261024037
2403	58	-0.5316526863965123
2403	59	-0.6549796563637713
2403	60	-0.7119129431897591
2403	61	-0.3760779331884976
2404	1	-0.04885824189488375
2404	2	0.06676473708742492
2404	3	-0.052501715688300976
2404	4	-0.0425065474978652
2404	5	0.06011990872347894
2404	6	0.11015568664473818
2404	7	0.09816361876264068
2404	8	0.1109775495853853
2404	9	0.023778948794834776
2404	10	0.06016084271932698
2404	11	-0.04547805353965373
2404	12	0.013203704772855929
2404	13	0.09726763248568915
2404	14	-0.022599732298090203
2404	15	0.01938360531573835
2404	16	-0.03492684293747317
2404	17	0.014334730866423229
2404	18	-0.007453683091355856
2404	19	-0.048349404135713314
2404	20	0.012783415286001798
2404	21	-0.07207819101962087
2404	22	-0.023053242415912223
2404	23	-0.018863787095725115
2404	24	0.038061994291361145
2404	25	-0.07327445055901194
2404	26	-0.03530591047730525
2404	27	0.0251296817784592
2404	28	-0.07626230235666043
2404	29	0.04935854282607721
2404	30	0.04738699815202807
2404	31	0.06249659677801844
2404	32	-0.019449637855601054
2404	33	0.004232666547480335
2404	34	0.08230517339452348
2404	35	-0.07598340872059595
2404	36	-0.13917098010247742
2404	37	-0.149560214223321
2404	38	-0.12437932191664913
2404	39	-0.09796328434925528
2404	40	-0.0985655783645143
2404	41	-0.08411338091270437
2404	42	-0.12719343262013894
2404	43	-0.03485534931056833
2404	44	0.007966320795240733
2404	45	0.056586372415075914
2404	46	-0.06284235498967661
2404	47	-0.1421712258634713
2404	48	-0.16055709464803414
2404	49	-0.0647248561364222
2404	50	0.3166757581064559
2404	51	-0.05172160648262292
2404	52	-0.17489773358088456
2404	53	-0.09513641595992794
2404	54	-0.13474219782311536
2404	55	-0.14125576848734056
2404	56	-0.1264842859192754
2404	57	-0.5934468316005308
2404	58	0.011407388923750261
2404	59	-0.12358715592481673
2404	60	0.12209818911617987
2404	61	-0.06992304120731774
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	6.0
14	25.0
15	105.0
16	403.0
17	735.0
18	1103.0
19	1235.0
20	1362.0
21	1402.0
22	1607.0
23	1947.0
24	2417.0
25	2909.0
26	3610.0
27	4560.0
28	7575.0
29	8961.0
30	9252.0
31	10684.0
32	15051.0
33	17396.0
34	22215.0
35	37112.0
36	55332.0
37	74205.0
38	137758.0
39	357113.0
40	280540.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.826074776261795	31.715837333616005	18.1470236382634	21.311064251858806
2	28.827582290700537	19.21447634911321	21.514546383751963	30.44339497643429
3	29.301262516325643	20.17111165792811	19.06891787018985	31.4587079555564
4	29.978989608373872	20.568510912153847	20.434877250099373	29.017622229372908
5	27.345119342810094	21.347693588991312	20.930608922791542	30.376578145407052
6	27.175711230148963	20.775207737881168	22.80649618595143	29.24258484601844
7	29.169048475326985	22.79864094944256	21.96626980371373	26.066040771516725
8	26.62272150820541	24.770210671764683	23.435104389468307	25.1719634305616
9	26.562529575438663	24.106679790274647	23.46983778463402	25.86095284965267
10	29.316878347939657	23.467661032348435	22.22416762885427	24.99129299085764
11	27.42670023281785	24.590675928905377	22.427552005451346	25.555071832825426
12	28.29210122844542	22.55456076924533	22.659328803164808	26.494009199144443
13	28.692150441975357	23.319263311313435	21.459370445382444	26.529215801328764
14	29.213529935076	23.22509511461074	21.761749730271998	25.79962522004126
15	27.87132554750052	22.63888625996101	22.96520981999205	26.52457837254642
16	28.950048267115896	23.11285040979728	21.089511839639606	26.84758948344722
17	29.550264049516382	23.273362230508603	21.294789044311106	25.88158467566391
18	27.741856107209784	22.600556491453883	22.882587874543354	26.774999526792982
19	30.18852567621283	23.298347561091028	20.845526300846092	25.66760046185005
20	28.24553765781454	23.298726126705912	22.644564744184287	25.811171471295264
21	28.128182317200128	24.080180197232686	21.479718347182526	26.31191913838466
22	30.234899964036266	23.641895856599344	20.807196532338967	25.31600764702542
23	29.581211788533246	23.760860101077018	21.216899169048475	25.44102894134126
24	29.19639984100244	23.210236414226497	21.447918835532167	26.145444909238897
25	29.030114894664116	25.27692074728852	20.60627283223865	25.086691525808714
26	28.537127822679864	25.462701822793438	21.08042626488236	24.91974408964434
27	28.124491302454995	25.097859211447826	21.140996763263992	25.636652722833187
28	29.133274024720336	25.929283943139446	20.33862694251481	24.598815089625408
29	28.840453521606634	26.030550245121237	20.629459976150365	24.499536257121765
30	28.45232912494558	25.64431867653461	20.692207226817587	25.211144971702222
31	29.661656981696353	25.275311843425264	21.139198576593287	23.923832598285095
32	30.31184342526168	25.50443868183453	20.290738392231834	23.892979500671956
33	29.055686635264323	25.160868400704516	21.504548986427334	24.278895977603828
34	29.843084552630085	26.014934413507223	20.756752664155513	23.38522836970718
35	29.673960364180125	25.975279665347994	19.969241543790577	24.381518426681303
36	30.08972005072779	25.480589048096764	20.62595824421268	23.80373265696277
37	30.60930135715773	26.19626734303723	20.312032708069125	22.88239859173591
38	31.088754708409834	26.18509965739812	19.98542522382692	22.740720410365125
39	30.686434101190592	25.87495977740342	19.267096969582255	24.171509151823738
40	31.376086010107702	26.443659972364706	18.77193314531241	23.408320872215178
41	31.312392345403268	26.376275292915146	19.47076527039049	22.8405670912911
42	31.21860271431546	25.96458518672749	20.368060419072137	22.448751679884914
43	32.372470708485544	26.374477106244438	19.760841172796276	21.492211012473735
44	31.92074728852378	26.581457856182922	20.247108705116315	21.25068615017698
45	32.55560182468626	26.093108212981015	19.83778463402169	21.51350532831103
46	33.516590638072344	26.04607143533153	17.96360091612879	22.47373701046734
47	33.486873237303854	25.959474550926537	18.212981014934414	22.340671196835192
48	33.23446461357915	27.520868429520544	18.36431261948477	20.880354337415532
49	34.64518937744885	26.261096704586322	17.546421608525296	21.547292309439534
50	33.61426056671273	27.53279324638943	18.368760765459673	20.48418542143817
51	33.65117071416403	27.442316064431864	18.35030569173402	20.556207529670083
52	34.09153716567924	28.010543052374555	18.085593685525545	19.812326096420662
53	34.59020272188677	26.219265204141507	17.412030815241053	21.77850125873067
54	33.58596278700006	25.868997368968977	17.56128030890954	22.983759535121425
55	34.858511101436655	26.179137248963674	18.900361530162215	20.06199011943745
56	33.159603263235596	27.304612822017376	21.1765819310632	18.359201983683825
57	34.23643315477655	28.03798905945373	19.622380799152015	18.103196986617707
58	32.77450739149363	29.044311105222313	20.523272321175067	17.657909182108988
59	32.46124434517613	29.238325982850977	19.651624992901894	18.648804679071
60	31.786072571028374	26.497416289678412	23.594575154738695	18.121935984554526
61	32.28369707179497	28.49652666048343	20.590278435009747	18.629497832711856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	3.5
2	3.5
3	4.0
4	3.0
5	2.0
6	2.0
7	5.5
8	9.0
9	10.5
10	12.0
11	11.0
12	16.5
13	23.0
14	28.0
15	33.0
16	37.5
17	64.5
18	87.0
19	110.5
20	190.0
21	246.0
22	292.5
23	339.0
24	451.0
25	734.5
26	906.0
27	1126.0
28	1346.0
29	1768.5
30	2631.5
31	3072.0
32	3738.0
33	4404.0
34	5268.0
35	7317.0
36	8502.0
37	10030.0
38	11558.0
39	13530.0
40	18022.0
41	20542.0
42	23577.0
43	30492.0
44	34372.0
45	38821.5
46	43271.0
47	48766.0
48	60315.0
49	66369.0
50	71167.5
51	75966.0
52	79316.0
53	84415.0
54	86164.0
55	85825.5
56	85487.0
57	83663.5
58	78804.0
59	75768.0
60	70841.5
61	60597.0
62	55279.0
63	49722.5
64	44166.0
65	38787.0
66	28641.5
67	23875.0
68	20283.5
69	16692.0
70	13856.5
71	8930.0
72	6839.0
73	5629.5
74	4420.0
75	3490.0
76	2033.5
77	1507.0
78	1195.5
79	884.0
80	673.5
81	371.0
82	279.0
83	204.5
84	100.0
85	70.0
86	63.5
87	57.0
88	46.5
89	38.0
90	40.0
91	35.5
92	31.0
93	37.5
94	50.0
95	56.0
96	49.0
97	42.0
98	58.5
99	181.0
100	287.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0026499593041963997
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0010410554409342999
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1056620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.75588880553201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.12189603808588	75.56971517974745
2	9.521817566764703	16.331038569640707
3	1.6888388807683636	4.344836378088927
4	0.3726059607453483	1.2781262135182607
5	0.11063794959813839	0.47439278517050043
6	0.054790972940195894	0.2819189149803811
7	0.03234736757179968	0.19417840796472477
8	0.022813550040486866	0.15651170084251456
9	0.020866553862027834	0.1610486885472033
>10	0.05020125582868381	0.6486053623686819
>50	0.0014150683530484518	0.08899032584658359
>100	0.0015329907158024895	0.2967018364021321
>500	1.1792236275403766E-4	0.0703832577150252
>1k	1.1792236275403766E-4	0.10355237916693365
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.217467017470803	0.0	0.0	0.0	0.0
2	1.2206848251973272	0.0	0.0	0.0	0.0
3	1.2226722946754747	0.0	0.0	0.0	0.0
4	1.2240919157312942	0.0	0.0	0.0	0.0
5	1.2254168953833924	0.0	9.464140372129999E-5	0.0	0.0
6	1.228823985917359	0.0	9.464140372129999E-5	0.0	0.0
7	1.2320417936438832	0.0	9.464140372129999E-5	0.0	0.0
8	1.245196948761144	0.0	9.464140372129999E-5	0.0	0.0
9	1.2495504533323238	0.0	9.464140372129999E-5	0.0	0.0
10	1.2571217656300278	0.0	9.464140372129999E-5	0.0	0.0
11	1.2711286933807802	0.0	9.464140372129999E-5	0.0	0.0
12	1.292517650621794	0.0	1.8928280744259999E-4	0.0	0.0
13	1.330658136321478	0.0	1.8928280744259999E-4	0.0	2.8392421116389997E-4
14	1.3778841967784066	0.0	1.8928280744259999E-4	0.0	2.8392421116389997E-4
15	1.4415778614828416	0.0	1.8928280744259999E-4	0.0	2.8392421116389997E-4
16	1.5121803486589314	0.0	1.8928280744259999E-4	0.0	2.8392421116389997E-4
17	1.6047396414983628	0.0	1.8928280744259999E-4	0.0	3.7856561488519997E-4
18	1.7406446972421494	0.0	1.8928280744259999E-4	0.0	3.7856561488519997E-4
19	1.9302114288959134	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
20	2.1924627586076357	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
21	2.5280611762033653	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
22	2.92517650621794	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
23	3.4307508848971247	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
24	4.056330563494917	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
25	4.73642369063618	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
26	5.557437867918457	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
27	6.485964679828132	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
28	7.519543449868449	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
29	8.641044083965854	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
30	9.904222899434044	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
31	11.20913857394333	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
32	12.639832673998221	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
33	14.183528610096346	0.0	1.8928280744259999E-4	0.0	4.732070186065E-4
34	15.769150688043005	0.0	1.8928280744259999E-4	0.0	5.678484223277999E-4
35	17.4126933050671	0.0	1.8928280744259999E-4	0.0	5.678484223277999E-4
36	19.169616323749313	0.0	1.8928280744259999E-4	0.0	5.678484223277999E-4
37	20.948874713709753	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
38	22.790217864511366	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
39	24.72695955026405	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
40	26.670326134277225	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
41	28.622872934451365	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
42	30.654161382521625	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
43	32.67257859968579	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
44	34.717873975506805	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
45	36.797145615263766	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
46	38.83089473983078	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
47	40.815335693059	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
48	42.87407014820844	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
49	44.84876303685336	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
50	46.85165906380723	9.464140372129999E-5	1.8928280744259999E-4	0.0	5.678484223277999E-4
>>END_MODULE