##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-21A_S1_R1_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1056620
Sequences flagged as poor quality	0
Sequence length	61
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.437239499536254	40.0	40.0	40.0	40.0	40.0
2	39.18024455338722	40.0	40.0	40.0	40.0	40.0
3	39.12754254131097	40.0	40.0	40.0	40.0	40.0
4	38.95238780261589	40.0	40.0	40.0	40.0	40.0
5	39.01968162631788	40.0	40.0	40.0	40.0	40.0
6	39.13694989684087	40.0	40.0	40.0	40.0	40.0
7	39.18934337794098	40.0	40.0	40.0	40.0	40.0
8	39.1942344456853	40.0	40.0	40.0	40.0	40.0
9	39.205197705892374	40.0	40.0	40.0	40.0	40.0
10	39.21029698472488	40.0	40.0	40.0	40.0	40.0
11	39.20289981261002	40.0	40.0	40.0	40.0	40.0
12	39.22392913251689	40.0	40.0	40.0	40.0	40.0
13	39.04714277602165	40.0	40.0	40.0	40.0	40.0
14	39.21488519997729	40.0	40.0	40.0	40.0	40.0
15	39.11830554030777	40.0	40.0	40.0	40.0	40.0
16	39.300399386723704	40.0	40.0	40.0	40.0	40.0
17	39.2580568226988	40.0	40.0	40.0	40.0	40.0
18	39.33875944048002	40.0	40.0	40.0	40.0	40.0
19	39.27871136264693	40.0	40.0	40.0	40.0	40.0
20	39.29562567432	40.0	40.0	40.0	40.0	40.0
21	39.27130661921978	40.0	40.0	40.0	40.0	40.0
22	39.33725274933278	40.0	40.0	40.0	40.0	40.0
23	39.32956029603831	40.0	40.0	40.0	40.0	40.0
24	39.32622134731503	40.0	40.0	40.0	40.0	40.0
25	39.285222691222955	40.0	40.0	40.0	40.0	40.0
26	39.24927788608961	40.0	40.0	40.0	40.0	40.0
27	39.26706289867691	40.0	40.0	40.0	40.0	40.0
28	39.27726902765422	40.0	40.0	40.0	40.0	40.0
29	39.28688837992845	40.0	40.0	40.0	40.0	40.0
30	39.28278851431924	40.0	40.0	40.0	40.0	40.0
31	39.23299577899339	40.0	40.0	40.0	40.0	40.0
32	39.258976737142966	40.0	40.0	40.0	40.0	40.0
33	39.26387726902765	40.0	40.0	40.0	40.0	40.0
34	39.261819764910754	40.0	40.0	40.0	40.0	40.0
35	39.259219019136495	40.0	40.0	40.0	40.0	40.0
36	39.227196153773356	40.0	40.0	40.0	40.0	40.0
37	39.238348696787874	40.0	40.0	40.0	40.0	40.0
38	39.23104238042059	40.0	40.0	40.0	40.0	40.0
39	38.00629365334747	40.0	40.0	40.0	40.0	40.0
40	39.23159130056217	40.0	40.0	40.0	40.0	40.0
41	39.23717135772558	40.0	40.0	40.0	40.0	40.0
42	39.18455830856883	40.0	40.0	40.0	40.0	40.0
43	39.221233745338914	40.0	40.0	40.0	40.0	40.0
44	39.19641498362704	40.0	40.0	40.0	40.0	40.0
45	39.02381934848858	40.0	40.0	40.0	40.0	40.0
46	39.04475781264788	40.0	40.0	40.0	40.0	40.0
47	39.113656754556985	40.0	40.0	40.0	40.0	40.0
48	39.22222558724991	40.0	40.0	40.0	40.0	40.0
49	39.207995305786376	40.0	40.0	40.0	40.0	40.0
50	39.19993185818932	40.0	40.0	40.0	40.0	40.0
51	39.162162366792224	40.0	40.0	40.0	40.0	40.0
52	39.17408339800496	40.0	40.0	40.0	40.0	40.0
53	39.10200071927467	40.0	40.0	40.0	40.0	40.0
54	39.03197743748935	40.0	40.0	40.0	40.0	40.0
55	39.066760046185	40.0	40.0	40.0	40.0	40.0
56	39.0718896102667	40.0	40.0	40.0	40.0	40.0
57	39.006022978932826	40.0	40.0	40.0	40.0	40.0
58	38.97287955934962	40.0	40.0	40.0	40.0	40.0
59	38.9065207927164	40.0	40.0	40.0	40.0	40.0
60	38.87281331036702	40.0	40.0	40.0	40.0	40.0
61	38.738486873237306	40.0	40.0	40.0	40.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.061563717497890025
1101	2	-0.07706751956369828
1101	3	0.10541024027560297
1101	4	0.023354771980237388
1101	5	0.07016818339792508
1101	6	0.08664503079226193
1101	7	-9.749331215260781E-4
1101	8	0.03056543628130015
1101	9	0.017113334870856534
1101	10	0.020859257178358348
1101	11	-0.07483760396816308
1101	12	0.011628104103692749
1101	13	0.05198202791923734
1101	14	-0.018694686005630956
1101	15	-0.009099359889958691
1101	16	0.05944455953778771
1101	17	0.0966697905115339
1101	18	0.1386910911296937
1101	19	0.14639738298863136
1101	20	0.07882195092727784
1101	21	0.05349637716288669
1101	22	0.1140550322856484
1101	23	0.08193471276498343
1101	24	0.0028646484693339858
1101	25	0.05723381663344185
1101	26	0.18952191921426476
1101	27	0.07688525834566207
1101	28	0.09387767419158166
1101	29	0.07128400666604051
1101	30	0.04397818706800649
1101	31	0.08915151372895735
1101	32	0.15046189777147134
1101	33	0.1576994489904635
1101	34	-0.005618555717056495
1101	35	0.13735101683904816
1101	36	-0.0892616072561907
1101	37	0.09787930239070164
1101	38	-0.04585827012604682
1101	39	1.2512989762958924
1101	40	0.10329777093537729
1101	41	0.10505136427377693
1101	42	0.025847660004977513
1101	43	-0.017249682814266976
1101	44	0.015018750051218888
1101	45	0.004034783899754757
1101	46	0.15333121221517843
1101	47	0.015515911567149487
1101	48	0.01484427297187807
1101	49	-0.020567733905856755
1101	50	0.053310202073454604
1101	51	0.10342955802419596
1101	52	0.1143476336390492
1101	53	0.04304254870250901
1101	54	-0.030170499014644747
1101	55	0.001252643620709648
1101	56	0.1463655303423934
1101	57	0.12069751367494064
1101	58	0.08649480740081827
1101	59	0.03986714971744476
1101	60	0.037167920165821045
1101	61	0.024011035196735975
1102	1	0.10111563066111273
1102	2	-0.09204367824206372
1102	3	0.09336828969664168
1102	4	-0.0074241126436902505
1102	5	-0.0333711698889374
1102	6	-0.07112678907737546
1102	7	-0.03735657677484738
1102	8	-0.07003970890615818
1102	9	-0.0881411631119633
1102	10	-0.08333225979630754
1102	11	-0.09539362019580011
1102	12	-0.061166892944662266
1102	13	-0.09094117689979697
1102	14	-0.059626325263636204
1102	15	-0.08728571768964599
1102	16	-0.039156882304162366
1102	17	-0.0677977174850497
1102	18	0.06439305232436965
1102	19	-0.023037279881322092
1102	20	-0.06366365945618213
1102	21	-0.06099375925770545
1102	22	-0.027602601508760927
1102	23	-0.1692878299017977
1102	24	-0.07821892273110365
1102	25	0.06966086440892383
1102	26	-0.08484803023864629
1102	27	-0.034001946755942924
1102	28	-0.04033909254574297
1102	29	-0.03185087124881392
1102	30	-1.4556618862826554E-4
1102	31	-0.07572998256215158
1102	32	-0.01060456426166212
1102	33	0.021820387126695096
1102	34	-0.08178465063946305
1102	35	-0.10904001818277465
1102	36	-0.12152380313114008
1102	37	-0.11236333174458935
1102	38	-0.06430805484332325
1102	39	1.0937589898711835
1102	40	-0.09962308383946805
1102	41	-0.1382997635096075
1102	42	-0.09160980357858506
1102	43	-0.07606350241296411
1102	44	-0.16448657445482695
1102	45	-0.04967623359714679
1102	46	-0.15078317962104393
1102	47	-0.15566771972324744
1102	48	-0.035121119368348275
1102	49	-0.12379562706304625
1102	50	-0.1265129245105996
1102	51	-0.09703097312454645
1102	52	-0.08300992311831124
1102	53	-0.1563021518685659
1102	54	-0.07147666555526655
1102	55	-0.18299504892303986
1102	56	-0.06538402118496833
1102	57	-0.1317275328635077
1102	58	-0.2275940598549795
1102	59	-0.2352983619218847
1102	60	-0.186957238889363
1102	61	-0.16298236864849258
1103	1	0.06745231339550628
1103	2	0.1296856995489719
1103	3	-0.04919798550844234
1103	4	-0.044861152099578305
1103	5	-0.006508004401766243
1103	6	0.0872658499147434
1103	7	9.135216965958648E-4
1103	8	0.001819188471692712
1103	9	0.0259808919848723
1103	10	0.03445349746048976
1103	11	0.03187846478746792
1103	12	0.1545433698327301
1103	13	-0.06351736621017068
1103	14	-0.03919142276229337
1103	15	0.07413581140247771
1103	16	0.004488028575217129
1103	17	0.08995378241662166
1103	18	-0.08118910741803376
1103	19	-0.027497484880619538
1103	20	0.009693689644578285
1103	21	-0.11195763841634232
1103	22	0.07066286065533234
1103	23	0.08218948778174706
1103	24	-0.041437433935129775
1103	25	0.04817110532290769
1103	26	-0.0032353123449411214
1103	27	0.1172502868176366
1103	28	-0.03168793284498861
1103	29	-1.7986558664517815E-4
1103	30	0.051442228477441176
1103	31	0.046044967423554795
1103	32	0.07065700343941472
1103	33	0.06729556732842923
1103	34	0.027881065976707475
1103	35	0.012928257734806436
1103	36	-0.009638001813613073
1103	37	0.13356201667706813
1103	38	0.011308044145181384
1103	39	1.2201256064898658
1103	40	-0.04569604539766203
1103	41	0.03246960164142365
1103	42	0.04769418440146467
1103	43	0.10455634621985865
1103	44	0.07565797710194744
1103	45	-0.009066891349966966
1103	46	0.027013758904651297
1103	47	-8.498527557065927E-5
1103	48	0.15068337925578135
1103	49	0.047167064019859595
1103	50	0.06087684675494387
1103	51	0.08282189881126101
1103	52	0.15360309091557411
1103	53	0.007358794518111722
1103	54	0.17090730329606174
1103	55	0.13638149955176715
1103	56	0.09291511835645849
1103	57	0.009822199441593682
1103	58	0.035718319876515636
1103	59	0.08934965746273349
1103	60	0.11611455199243892
1103	61	0.1715611264347885
1104	1	0.05378971122313203
1104	2	-0.008954833010228924
1104	3	-0.061307163323363056
1104	4	-0.09327367748179682
1104	5	-0.09025091405265329
1104	6	-0.04132113165665885
1104	7	0.045097664675211036
1104	8	-0.06797399809021698
1104	9	0.02547644368373625
1104	10	0.02102722586207051
1104	11	-0.0920102373327012
1104	12	-0.09168406517549954
1104	13	-0.1472302162705148
1104	14	-0.07138282565029641
1104	15	0.054866231811764976
1104	16	-0.09243480223662459
1104	17	0.011797119431967928
1104	18	0.014648123008761615
1104	19	-9.873446059884827E-4
1104	20	-0.006157388924620477
1104	21	-0.04894928150465461
1104	22	-0.00754251959676111
1104	23	-0.10528872333401296
1104	24	-0.026710307642353825
1104	25	-0.061708791064788215
1104	26	-0.03900484304563179
1104	27	-0.007937887946752653
1104	28	0.05827489346136616
1104	29	-0.054800560198138726
1104	30	0.07788084765732606
1104	31	-0.040279266376487044
1104	32	0.004252258308461876
1104	33	-0.05550926196936956
1104	34	0.014404004035640128
1104	35	0.04529829060341939
1104	36	-0.0054338052874598475
1104	37	-0.1643516749827043
1104	38	-0.13022045493800505
1104	39	1.1702830047251993
1104	40	-0.10726361503830617
1104	41	0.028400500516468696
1104	42	-0.11379319177979852
1104	43	-0.05584819367664551
1104	44	0.021062159392975843
1104	45	-0.10459281498271622
1104	46	0.06308215794813066
1104	47	0.033595922150908564
1104	48	-0.0027131298928679826
1104	49	-0.05794925104748927
1104	50	-0.005478144570965071
1104	51	-0.029316958530415604
1104	52	-0.05851569214781449
1104	53	0.08567750288169407
1104	54	-0.08222243562430265
1104	55	-0.05326756682153899
1104	56	-0.05088892628238284
1104	57	-0.036322036148206394
1104	58	0.020117046870133493
1104	59	0.04321578724898956
1104	60	0.08642880600842062
1104	61	-0.004925639916848468
1201	1	0.019769630638649005
1201	2	0.055399378434842106
1201	3	0.023427800507548113
1201	4	-0.03628978842020558
1201	5	-0.010989414713094448
1201	6	0.11451349808194067
1201	7	-0.01651718608368924
1201	8	-0.03479992488710337
1201	9	-0.06484097730446337
1201	10	0.031843433737364535
1201	11	-0.01718975105718812
1201	12	-0.043926352717775785
1201	13	-0.007646007520470732
1201	14	-0.014952661386779198
1201	15	-0.03636273234086218
1201	16	-0.055516427930577095
1201	17	-0.004074105207529044
1201	18	-0.048883386560689246
1201	19	0.05724803259099076
1201	20	0.003524033573356178
1201	21	0.14342943261318908
1201	22	0.11223095789660675
1201	23	0.0949581397974768
1201	24	0.06411037208638248
1201	25	-0.012521206976600752
1201	26	0.056458013059867085
1201	27	0.030834763515379393
1201	28	-0.07216970199185369
1201	29	0.061514590518392254
1201	30	0.10394656637984667
1201	31	0.02980967654534794
1201	32	0.018365932402168994
1201	33	-0.043517965962465155
1201	34	0.1215329304438626
1201	35	0.08003190516819814
1201	36	0.010350856044986756
1201	37	0.05410222024345046
1201	38	0.011370130058224959
1201	39	1.371222374313021
1201	40	0.14911937747974946
1201	41	0.06754849328159906
1201	42	0.05820669747524221
1201	43	0.13088287795957143
1201	44	0.045090311042081055
1201	45	-0.053587050987935925
1201	46	-0.0267975400826046
1201	47	0.0755496875321029
1201	48	0.0679477616811539
1201	49	0.010499895462835696
1201	50	-0.00773089330642307
1201	51	-0.06643824844284296
1201	52	-0.038213968206264326
1201	53	-0.0849615551132672
1201	54	-0.04189970518629593
1201	55	0.20230656694084814
1201	56	-0.07644262479605146
1201	57	0.1581072120161835
1201	58	0.025490981081318864
1201	59	0.04420653408705988
1201	60	0.06147824697416837
1201	61	0.16206969863154086
1202	1	0.11107742218401029
1202	2	0.08326257180087993
1202	3	-0.02898410101528981
1202	4	0.024533669710855577
1202	5	-0.020606941859959704
1202	6	0.10904408767407858
1202	7	0.06622223752392387
1202	8	0.0529644931631168
1202	9	-0.07955627904014051
1202	10	-0.027475549777143726
1202	11	-0.018250512898312365
1202	12	-0.02138103235372313
1202	13	-0.09302239294724757
1202	14	0.0166722459130213
1202	15	-0.022067234926801405
1202	16	-0.09381214544305294
1202	17	-0.03300895985687191
1202	18	-0.07940498903535342
1202	19	-0.07169869717640864
1202	20	-0.052035697171746165
1202	21	-0.09032914597298003
1202	22	0.025637702489007097
1202	23	-0.06542750356275207
1202	24	0.060630637269809995
1202	25	-0.0382568995469299
1202	26	0.004434975506626415
1202	27	0.028550451390167098
1202	28	-0.049947848944285056
1202	29	0.021770301979920248
1202	30	0.0722717326029354
1202	31	0.02549103627537619
1202	32	0.013709761019335076
1202	33	0.07281881238569099
1202	34	-0.06692123770939418
1202	35	0.026534630160597317
1202	36	-0.30499989195999433
1202	37	-0.19448733480352587
1202	38	-0.2380186002174085
1202	39	1.1793862147279555
1202	40	-0.10772492284593937
1202	41	-0.11304471589127019
1202	42	-0.1321246358983501
1202	43	-0.07973126253722285
1202	44	-0.04156834101863183
1202	45	-0.11031829597056486
1202	46	-0.13314093632593682
1202	47	0.1298689914374691
1202	48	-0.0016602952568263163
1202	49	-0.047682381710153265
1202	50	-0.167143673552836
1202	51	0.005581046382580723
1202	52	-0.03890907134179855
1202	53	0.07958837835178656
1202	54	-0.045496169512730944
1202	55	-0.07655460660033242
1202	56	-0.08469842485949641
1202	57	-0.09739856649009937
1202	58	-0.09859213630682007
1202	59	0.026899274680602048
1202	60	-0.04653381870832618
1202	61	-0.09387873786545242
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2403	3	8.131153116863743E-5
2403	4	0.10482493492801837
2403	5	0.11791226838041524
2403	6	0.09771670284553835
2403	7	0.1292225176985511
2403	8	0.01376623455620063
2403	9	0.07480766589504384
2403	10	0.08675058306819494
2403	11	0.19054787985777466
2403	12	0.07856992571893073
2403	13	0.10611820479318368
2403	14	0.10980844033766601
2403	15	0.07649967320293172
2403	16	-0.032120983729548414
2403	17	-0.0026878993727095235
2403	18	0.08945892511790277
2403	19	0.07139351592399379
2403	20	-0.12019097229805453
2403	21	0.0891252055227838
2403	22	0.044927830366312094
2403	23	0.11388062241998398
2403	24	0.018525328165168276
2403	25	0.003430466486904038
2403	26	0.02037028272070529
2403	27	0.04222071813259731
2403	28	0.08336362331110792
2403	29	-0.03919918953994994
2403	30	0.07041481408555228
2403	31	0.045184193676135465
2403	32	0.05200847809961573
2403	33	-0.013294556218184539
2403	34	0.01761129487557156
2403	35	0.04480990932810869
2403	36	-0.0020038967021420717
2403	37	0.06030407422934303
2403	38	0.06088962808681231
2403	39	1.2890626243969194
2403	40	0.09954319445419912
2403	41	0.06992668656326373
2403	42	0.03704773406347073
2403	43	0.05958345814340049
2403	44	0.06486564520979954
2403	45	0.13443193075627846
2403	46	0.09609810992360934
2403	47	0.16488781933631458
2403	48	0.04144494901025553
2403	49	0.06799909510932878
2403	50	0.08510624876485906
2403	51	0.007053850119483229
2403	52	0.031457046242614695
2403	53	0.06212570564222375
2403	54	0.08786513581551247
2403	55	0.011159604000596346
2403	56	0.05562568835506454
2403	57	-0.032140520744981416
2403	58	0.048582387571364904
2403	59	0.06655330584428043
2403	60	0.13175218366347252
2403	61	0.04174033303328173
2404	1	-0.018226816474395946
2404	2	-0.05464729462870821
2404	3	-0.09724293094301117
2404	4	0.033495897762435334
2404	5	0.006966889867044301
2404	6	0.053762902653900824
2404	7	0.032056999700849076
2404	8	-0.022595686797906467
2404	9	0.08220672669315121
2404	10	0.1162555312753355
2404	11	0.1752743424668921
2404	12	0.09321955090755552
2404	13	0.12157281688514843
2404	14	0.046942561952057815
2404	15	0.05857327779601462
2404	16	0.036749149911535994
2404	17	0.02452255204689635
2404	18	-0.03155541899107561
2404	19	-0.055366729996663366
2404	20	0.04364715979897227
2404	21	0.05187457862739109
2404	22	0.0322128254672549
2404	23	0.05181745803351845
2404	24	0.04524634754754686
2404	25	-0.07148748640678093
2404	26	0.14200778139721137
2404	27	-0.019224216312110798
2404	28	0.01912188025118411
2404	29	0.030905131080459114
2404	30	0.01608520623160814
2404	31	0.09804209606210179
2404	32	0.07638348153264474
2404	33	-0.04995915264794348
2404	34	0.021359313897505672
2404	35	-0.04117708700815825
2404	36	0.08791825443500301
2404	37	-0.047730850458926
2404	38	0.0903044177607839
2404	39	1.3173205058342816
2404	40	0.052663787742808665
2404	41	0.056280169760540844
2404	42	0.03741518690875978
2404	43	0.1123769615770982
2404	44	0.08708782955028482
2404	45	-0.008724667228904082
2404	46	-0.13516004293639128
2404	47	0.10110974873271061
2404	48	-0.0014421014779557595
2404	49	0.054500695896813056
2404	50	0.07640291977467228
2404	51	0.015096900440255467
2404	52	0.07845312870281873
2404	53	-0.0195855790808821
2404	54	0.0052094593982729975
2404	55	-0.013418445540757773
2404	56	-0.04911655578553109
2404	57	0.06524698422936837
2404	58	-0.027483760759501763
2404	59	0.09023734640122427
2404	60	0.08891892524127343
2404	61	0.021040994505220567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	37.0
18	110.0
19	134.0
20	213.0
21	273.0
22	410.0
23	586.0
24	854.0
25	1130.0
26	1477.0
27	1937.0
28	2647.0
29	3060.0
30	3925.0
31	4922.0
32	6644.0
33	7963.0
34	10193.0
35	12855.0
36	19007.0
37	26802.0
38	69318.0
39	456829.0
40	425291.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.838617478374438	11.48946641176582	32.711570858018966	29.960345251840774
2	28.76002934841779	16.665830394546873	28.41930368511988	26.154836571915457
3	27.728848079438357	20.32437456109966	21.018192005057646	30.928585354404337
4	26.278983929889648	20.151142321742917	21.413564005981335	32.1563097423861
5	27.29533796445269	20.289697336790898	20.142908519619162	32.27205617913725
6	29.409342999375365	22.704094187124984	22.92735325850353	24.95920955499612
7	27.468910298877553	22.177320134012227	22.718101114875736	27.635668452234484
8	25.224584051030646	24.506823645208307	23.12600556491454	27.142586738846507
9	25.605894266623764	24.603357877004033	23.39781567640211	26.392932179970092
10	26.12670591130208	22.240162026083173	23.521890556680734	28.111241505934014
11	25.187484620771894	23.337055895213037	23.241657360261968	28.233802123753097
12	27.205996479339785	22.6672786810774	22.852113342545096	27.274611497037725
13	25.511158221498743	21.454922299407546	23.03732656962768	29.996592909466035
14	25.897200507277923	22.754159489693553	22.949404705570593	28.39923529745793
15	26.282391020423614	22.09753743067517	22.759364766898223	28.860706782002993
16	25.777100565955596	22.6832730783063	22.821260244931953	28.718366110806155
17	26.10607408529083	21.55931176771214	22.67977134636861	29.654842800628415
18	27.903882190380646	22.18602714315459	22.45036058374818	27.45973008271659
19	26.00991841910999	21.45454373379266	23.806098692055798	28.729439155041547
20	26.065851488709278	21.906929643580472	22.584183528610097	29.44303533910015
21	26.53091934659575	22.414302208930362	23.547254452878043	27.507523991595846
22	27.12857981109576	21.718120043156482	22.766273589369877	28.387026556377887
23	26.142511025723536	23.305256383562682	22.543960932028543	28.008271658685242
24	26.643258692812932	23.91881660388787	22.347485377903126	27.090439325396076
25	26.046450000946415	22.073687796937406	22.65374496034525	29.226117241770933
26	26.315042304707465	22.431243020196476	23.695652173913043	27.558062501183016
27	26.64231227877572	23.17985652363196	22.262497397361397	27.915333800230925
28	26.13115405727698	22.824761976869638	22.44771062444398	28.596373341409397
29	26.614203781870494	24.21816736385834	22.190191364918324	26.97743748935284
30	28.21212924230092	23.832503643694043	21.86282674944635	26.092540364558687
31	26.516344570422667	23.369423255285724	23.348602146467037	26.76563002782457
32	26.932009615566617	23.708050197800535	21.7764191478488	27.58352103878405
33	27.172140572902816	25.643017965794673	21.672334114756598	25.512507346545917
34	26.859703583123544	24.2090817891011	21.528553311502716	27.402661316272642
35	27.15659366659726	25.73280838901403	21.23753099505972	25.873066949328994
36	28.78139728568454	25.37364426189169	20.771800647347202	25.073157805076562
37	27.248301186803204	26.243304120686716	20.86275103632337	25.645643656186706
38	27.39953814994984	25.458537601029697	21.639662319471523	25.502261929548943
39	28.066217183689297	26.016000500076668	19.968393591595586	25.949388724638446
40	28.5048551040109	25.945656905983228	20.05366167590998	25.495826314095893
41	27.633870265563782	26.059131949045067	21.00121140996763	25.305786375423523
42	27.86782381556283	26.725691355454185	19.498968408699437	25.907516420283542
43	27.395941776608428	27.808294372622132	19.56247279059643	25.233291060173002
44	27.61882228237209	26.778311975923224	20.480115841078156	25.12274990062653
45	27.71204406503757	27.39632034222332	18.99187976756071	25.8997558251784
46	27.265904487895366	27.202494747402095	20.115462512539985	25.416138252162558
47	28.660350930324995	27.35600310423804	18.67492570649808	25.308720258938884
48	28.698112850409796	27.732108042626493	18.63337813026443	24.936400976699286
49	28.299388616531964	27.609452783403682	18.517158486494672	25.574000113569685
50	28.298158278283587	27.802710529802578	18.369139331074557	25.529991860839278
51	28.245159092199657	28.13045371088944	18.241752001665688	25.382635195245218
52	27.661789479661564	28.182885048551036	18.1889420983892	25.966383373398195
53	27.795990990138364	28.23276106831217	18.00013249796521	25.97111544358426
54	27.651473566655945	28.46945921901914	17.979879237568852	25.89918797675607
55	27.265715205087922	28.45280233196419	17.93085499044122	26.350627472506673
56	27.097821354886335	28.59466979614242	17.824288769851034	26.483220079120212
57	26.999772860631072	28.64833147205239	17.831197592322688	26.52069807499385
58	26.401923113323615	28.79067214324923	17.864511366432588	26.94289337699457
59	26.5347050027446	28.692150441975357	17.776021653953173	26.99712290132687
60	26.23819348488577	29.053775245594444	17.735988340179063	26.972042929340727
61	26.269141223902633	28.837141072476385	17.737975809657208	27.15574189396377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.0
2	2.0
3	3.0
4	4.5
5	6.0
6	6.0
7	6.5
8	7.0
9	6.5
10	6.0
11	10.0
12	18.5
13	23.0
14	26.0
15	29.0
16	40.5
17	66.5
18	81.0
19	113.5
20	177.0
21	208.0
22	279.5
23	351.0
24	454.5
25	728.0
26	898.0
27	1156.5
28	1415.0
29	1793.5
30	2602.0
31	3032.0
32	3782.5
33	4533.0
34	5364.0
35	7607.5
36	9020.0
37	10413.5
38	11807.0
39	14013.0
40	18815.0
41	21411.0
42	24190.5
43	30786.0
44	34602.0
45	39023.5
46	43445.0
47	48404.0
48	58798.5
49	64234.0
50	69763.0
51	75292.0
52	79022.5
53	85117.0
54	87481.0
55	88001.0
56	88521.0
57	86661.0
58	80692.0
59	76583.0
60	71419.0
61	64895.5
62	63536.0
63	51904.0
64	40272.0
65	34921.0
66	25312.5
67	21055.0
68	17794.5
69	14534.0
70	12045.0
71	7880.5
72	6205.0
73	5051.5
74	3898.0
75	3131.0
76	1889.0
77	1414.0
78	1105.5
79	797.0
80	609.0
81	337.5
82	254.0
83	180.0
84	81.5
85	57.0
86	46.0
87	35.0
88	26.5
89	14.0
90	10.0
91	7.0
92	4.0
93	4.0
94	3.5
95	3.0
96	4.0
97	5.0
98	5.5
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.032651284283848496
3	1.8928280744259999E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	9.464140372129999E-5
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	3.10206128977305
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1056620.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.16146186401295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.61621560769815	67.86414294257278
2	13.077895349255177	21.22842209300253
3	2.4107124166835883	5.869708316153026
4	0.5307640567211147	1.7231034699343826
5	0.15465735600117209	0.6276108550539101
6	0.07616672689514266	0.3709081740124086
7	0.03755616919504064	0.21336795157171962
8	0.02577196464753397	0.16733522607212145
9	0.017674212442861353	0.12910184272419795
>10	0.05156956952248952	0.5842764578604656
>50	5.082854688602686E-4	0.02815530333315788
>100	3.8121410164520137E-4	0.03630280869330247
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.2707136721506714E-4	1.1575645590159855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGTCATCTGGGGGGGGGGGGGGG	11224	1.0622551153678712	TruSeq Adapter, Index 14 (97% over 38bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2181295072968523	0.0	0.0	0.0	0.0
2	1.2219151634457042	0.0	0.0	0.0	0.0
3	1.2245651227499006	0.0	0.0	0.0	0.0
4	1.2264579508243267	0.0	0.0	0.0	0.0
5	1.2290132687248017	0.0	0.0	0.0	0.0
6	1.2334614146997027	0.0	0.0	0.0	0.0
7	1.2396131059415874	0.0	0.0	0.0	0.0
8	1.2894891257027123	0.0	0.0	0.0	0.0
9	1.2949783271185478	0.0	0.0	0.0	0.0
10	1.3027389222236945	0.0	0.0	0.0	0.0
11	1.3151369461111848	0.0	0.0	0.0	0.0
12	1.3362419791410347	0.0	0.0	0.0	0.0
13	1.3757074444928168	0.0	0.0	0.0	0.0
14	1.4209460354715981	0.0	0.0	0.0	9.464140372129999E-5
15	1.4822736650830004	0.0	0.0	0.0	9.464140372129999E-5
16	1.5501315515511727	0.0	0.0	0.0	9.464140372129999E-5
17	1.6400408850864077	0.0	0.0	0.0	9.464140372129999E-5
18	1.768279987128769	0.0	0.0	0.0	9.464140372129999E-5
19	1.9576574359750905	0.0	0.0	0.0	9.464140372129999E-5
20	2.2182998618235508	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
21	2.553235789593231	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
22	2.9559349624273628	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
23	3.461793265317711	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
24	4.089928261815979	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
25	4.776173080199126	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
26	5.602108610474911	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
27	6.532717533266453	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
28	7.540648482898298	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
29	8.632526357630937	0.0	9.464140372129999E-5	0.0	9.464140372129999E-5
30	9.862107474778066	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
31	11.125759497264863	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
32	12.51594707652704	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
33	14.016865098143136	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
34	15.557248585111015	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
35	17.151766955007478	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
36	18.857867539891352	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
37	20.604474645567944	0.0	9.464140372129999E-5	0.0	3.7856561488519997E-4
38	22.415816471389903	0.0	9.464140372129999E-5	0.0	4.732070186065E-4
39	24.302966061592624	0.0	9.464140372129999E-5	0.0	4.732070186065E-4
40	26.258257462474685	0.0	9.464140372129999E-5	0.0	4.732070186065E-4
41	28.22594688724423	0.0	9.464140372129999E-5	0.0	4.732070186065E-4
42	30.269728000605706	0.0	9.464140372129999E-5	0.0	4.732070186065E-4
43	32.30953417501088	0.0	9.464140372129999E-5	0.0	5.678484223277999E-4
44	34.38227555791107	0.0	9.464140372129999E-5	0.0	5.678484223277999E-4
45	36.49930911775284	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
46	38.566277375026026	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
47	40.588480248339046	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
48	42.67002328178531	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
49	44.66373909257822	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
50	46.68291344097216	0.0	9.464140372129999E-5	0.0	6.624898260490999E-4
>>END_MODULE