##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-21A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 666985 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.54373786516938 40.0 40.0 40.0 40.0 40.0 2 39.3418592622023 40.0 40.0 40.0 40.0 40.0 3 39.303540559382895 40.0 40.0 40.0 40.0 40.0 4 39.134823121959265 40.0 40.0 40.0 40.0 40.0 5 39.196065878542996 40.0 40.0 40.0 40.0 40.0 6 39.324398599668655 40.0 40.0 40.0 40.0 40.0 7 39.371248228970664 40.0 40.0 40.0 40.0 40.0 8 39.38370428120572 40.0 40.0 40.0 40.0 40.0 9 39.39025315411891 40.0 40.0 40.0 40.0 40.0 10-11 39.39417827987136 40.0 40.0 40.0 40.0 40.0 12-13 39.32214967353089 40.0 40.0 40.0 40.0 40.0 14-15 39.3539764762326 40.0 40.0 40.0 40.0 40.0 16-17 39.46204187500469 40.0 40.0 40.0 40.0 40.0 18-19 39.4941985202066 40.0 40.0 40.0 40.0 40.0 20-21 39.474518917217026 40.0 40.0 40.0 40.0 40.0 22-23 39.51529944451525 40.0 40.0 40.0 40.0 40.0 24-25 39.487322803361394 40.0 40.0 40.0 40.0 40.0 26-27 39.46098338043584 40.0 40.0 40.0 40.0 40.0 28-29 39.47789230642368 40.0 40.0 40.0 40.0 40.0 30-31 39.45675852679243 40.0 40.0 40.0 40.0 40.0 32-33 39.46059019039872 40.0 40.0 40.0 40.0 40.0 34-35 39.45386975441076 40.0 40.0 40.0 40.0 40.0 36-37 39.43708974850402 40.0 40.0 40.0 40.0 40.0 38-39 39.37904452803195 40.0 40.0 40.0 40.0 40.0 40-41 39.42407959706445 40.0 40.0 40.0 40.0 40.0 42-43 39.402294576775155 40.0 40.0 40.0 40.0 40.0 44-45 39.305268153840174 40.0 40.0 40.0 40.0 40.0 46-47 39.28983723827622 40.0 40.0 40.0 40.0 40.0 48-49 39.367084864407836 40.0 40.0 40.0 40.0 40.0 50-51 39.33163784435929 40.0 40.0 40.0 40.0 40.0 52-53 39.286580112739315 40.0 40.0 40.0 40.0 40.0 54-55 39.198475192515055 40.0 40.0 40.0 40.0 40.0 56-57 39.21765933556011 40.0 40.0 40.0 40.0 40.0 58-59 39.15881881236802 40.0 40.0 40.0 40.0 40.0 60-61 39.08036396591602 40.0 40.0 40.0 40.0 40.0 62-63 39.35687420146698 40.0 40.0 40.0 40.0 40.0 64-65 39.36778922802582 40.0 40.0 40.0 40.0 40.0 66-67 39.36282114105934 40.0 40.0 40.0 40.0 40.0 68-69 39.35849371487379 40.0 40.0 40.0 40.0 40.0 70-71 39.353125389044855 40.0 40.0 40.0 40.0 40.0 72-73 39.37286954201903 40.0 40.0 40.0 40.0 40.0 74-75 39.36298835874709 40.0 40.0 40.0 40.0 40.0 76-77 39.36108193477595 40.0 40.0 40.0 40.0 40.0 78-79 39.3755227644648 40.0 40.0 40.0 40.0 40.0 80-81 39.35983308992657 40.0 40.0 40.0 40.0 40.0 82-83 39.33898952561651 40.0 40.0 40.0 40.0 40.0 84-85 39.332829915963664 40.0 40.0 40.0 40.0 40.0 86-87 39.32955003264459 40.0 40.0 40.0 40.0 40.0 88-89 39.34436386719695 40.0 40.0 40.0 40.0 40.0 90-91 39.39872552340281 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.013887037776669331 1101 2 -0.07415295419310297 1101 3 -0.01455410182416017 1101 4 -0.07965379900816316 1101 5 -0.12519841057916636 1101 6 -0.019972853131676516 1101 7 -0.016660908345642156 1101 8 0.00971203554319544 1101 9 0.11287663443201268 1101 10-11 -9.752184934441743E-4 1101 12-13 -0.01435760673926012 1101 14-15 -0.002299739019171909 1101 16-17 0.003658359114972143 1101 18-19 -0.008682016511485813 1101 20-21 0.004664264317973732 1101 22-23 0.09501437338712293 1101 24-25 -0.0010840858848908397 1101 26-27 -0.03574512862763868 1101 28-29 0.02076252698078207 1101 30-31 0.0692364631413156 1101 32-33 -0.07618238709135028 1101 34-35 0.06656145071350039 1101 36-37 -0.19092878998780805 1101 38-39 -0.0817841606483185 1101 40-41 -0.027947786638556238 1101 42-43 -0.0012944927041758092 1101 44-45 -0.03545357103000413 1101 46-47 0.005828708782381398 1101 48-49 0.14196686326378227 1101 50-51 0.05179551579830388 1101 52-53 0.10611751407676451 1101 54-55 0.05214491201969906 1101 56-57 0.18600341760572547 1101 58-59 0.02990907420254274 1101 60-61 -0.03911419372602154 1101 62-63 -0.07938350152976881 1101 64-65 0.08319378279519896 1101 66-67 -0.003868344608427776 1101 68-69 -0.12126371424061944 1101 70-71 0.08347302631826636 1101 72-73 0.0846677895060779 1101 74-75 -0.22182316189929452 1101 76-77 0.03751248251866457 1101 78-79 0.16496499317419477 1101 80-81 -0.12013925120817248 1101 82-83 0.28808962384430714 1101 84-85 -0.005984761316859988 1101 86-87 0.03252451337324658 1101 88-89 -0.13619638819811541 1101 90-91 0.515384390398907 1102 1 -0.04135227414933951 1102 2 0.07623706654604945 1102 3 0.19670997474248253 1102 4 0.15929933850899403 1102 5 -0.007786346110165709 1102 6 -0.0974876369821942 1102 7 0.07097524012456802 1102 8 -0.10888814820356885 1102 9 -0.1336381176569006 1102 10-11 -0.05876722233306708 1102 12-13 -0.033252232322880104 1102 14-15 -0.013464531163002391 1102 16-17 -0.0409537552315129 1102 18-19 -0.045646642318615704 1102 20-21 0.024148244117597528 1102 22-23 0.0713637416252837 1102 24-25 0.06943373413974285 1102 26-27 -0.0152930080699889 1102 28-29 -0.03134885508426066 1102 30-31 -0.0892504140224375 1102 32-33 -0.007157081871802973 1102 34-35 -0.006824154653209291 1102 36-37 0.04760267283626263 1102 38-39 0.06771430445196813 1102 40-41 0.03212141661872181 1102 42-43 -0.03188927553372878 1102 44-45 -0.004039712344855673 1102 46-47 -0.011535980021371017 1102 48-49 -0.07866123048975737 1102 50-51 0.10042965469578036 1102 52-53 0.1735076633413044 1102 54-55 -0.0022597646555695405 1102 56-57 0.08728804131192192 1102 58-59 0.03755358697885214 1102 60-61 -0.18978678228381796 1102 62-63 -0.07110698040168728 1102 64-65 -0.1294320742659636 1102 66-67 -0.05032171957426357 1102 68-69 -0.02005252184040529 1102 70-71 -0.04575775180827435 1102 72-73 -0.18185583038342656 1102 74-75 -0.06635218575880941 1102 76-77 -0.14919229874161033 1102 78-79 -0.418534009563615 1102 80-81 0.09602114979433907 1102 82-83 -0.0723127445028382 1102 84-85 -0.516111740927407 1102 86-87 -0.056373386523858926 1102 88-89 0.06516891316718443 1102 90-91 0.515384390398907 1103 1 0.0547397860043759 1103 2 0.15575693112323563 1103 3 -0.12475847362329517 1103 4 -0.037258665030464044 1103 5 -0.0018530486843957306 1103 6 -0.02362748839328077 1103 7 -0.040823814309590034 1103 8 -0.04052014697245454 1103 9 -0.01799866379384696 1103 10-11 -0.0019306786925525898 1103 12-13 -0.018894336506107834 1103 14-15 -0.04227778618365363 1103 16-17 0.04150027026285841 1103 18-19 -0.03345345962605961 1103 20-21 -0.007340979338593456 1103 22-23 0.07038226821825333 1103 24-25 0.03622094762730654 1103 26-27 -0.0964663304600677 1103 28-29 0.02671538525703454 1103 30-31 0.02540927631756773 1103 32-33 0.02623158393346614 1103 34-35 0.01791326667263604 1103 36-37 0.04027554051562987 1103 38-39 -0.047268660071758006 1103 40-41 0.01911010193376228 1103 42-43 0.07822561137524531 1103 44-45 0.007664295628138973 1103 46-47 -0.007627530370335478 1103 48-49 -0.02783756657252212 1103 50-51 -0.0010753438120758574 1103 52-53 -0.10709109401597772 1103 54-55 -0.10395165302940512 1103 56-57 0.10076771841418264 1103 58-59 -0.08541118088567146 1103 60-61 0.0606717415534348 1103 62-63 0.05855821634117575 1103 64-65 -0.09204518804332196 1103 66-67 -0.18032518211395399 1103 68-69 0.04955540848155238 1103 70-71 -0.1845223506256488 1103 72-73 0.1398581566848165 1103 74-75 -0.11631110476944428 1103 76-77 -0.05483116331647153 1103 78-79 -0.20740900963458841 1103 80-81 0.05635691510124019 1103 82-83 -0.1350162646093196 1103 84-85 0.3047094827107131 1103 86-87 0.03668829995038436 1103 88-89 0.297023906571674 1103 90-91 0.515384390398907 1104 1 -0.04321231509732826 1104 2 0.054818379003584994 1104 3 0.06632200722096826 1104 4 -0.33794784603460215 1104 5 0.07889517379611277 1104 6 0.13088656533336973 1104 7 0.06556215979291835 1104 8 0.09079311662427614 1104 9 0.11732745578424186 1104 10-11 0.023884961296317897 1104 12-13 -0.07845529055524736 1104 14-15 -0.0303516262259933 1104 16-17 -0.11358564545297867 1104 18-19 0.009370163512528507 1104 20-21 -0.007209472907867109 1104 22-23 -0.045054731470713705 1104 24-25 -0.04938428207241685 1104 26-27 0.06476437329031626 1104 28-29 0.09809554797578102 1104 30-31 -0.07957323496415114 1104 32-33 -0.08007587442908459 1104 34-35 -0.047785668750897514 1104 36-37 -0.12205938222157897 1104 38-39 0.048051938684928075 1104 40-41 -0.1661363749675644 1104 42-43 -0.030135725630088928 1104 44-45 0.047325985060133746 1104 46-47 0.0025033005684989007 1104 48-49 -0.04619827590178005 1104 50-51 0.02416078789278231 1104 52-53 0.09796585457166884 1104 54-55 0.07060913548266967 1104 56-57 0.15899243809337094 1104 58-59 -0.027541487198050163 1104 60-61 -0.08658312343883523 1104 62-63 0.07086303339824696 1104 64-65 0.21091696698373852 1104 66-67 0.003356522722782529 1104 68-69 -0.07817838237154007 1104 70-71 0.07952046238323618 1104 72-73 0.08593654085416347 1104 74-75 0.19223736009493564 1104 76-77 -0.13288601444824621 1104 78-79 0.1386567690242515 1104 80-81 -0.0474723331756195 1104 82-83 0.04640306876559919 1104 84-85 -0.35174780572985753 1104 86-87 -0.24070518375889094 1104 88-89 0.45157790647272833 1104 90-91 0.515384390398907 1201 1 0.03443824746588575 1201 2 0.15668452824930057 1201 3 0.13931269958698778 1201 4 0.012124627383556685 1201 5 -0.05316823301583895 1201 6 -0.029549882027374963 1201 7 -0.08882988342919163 1201 8 -0.10121356611824694 1201 9 -0.006571823223559647 1201 10-11 0.0659217449953573 1201 12-13 -0.03545769753119288 1201 14-15 -0.014168647629041686 1201 16-17 -0.07713212143989523 1201 18-19 0.07520101044448069 1201 20-21 0.05055519814424514 1201 22-23 0.0710958899737193 1201 24-25 -0.03674243062695837 1201 26-27 0.027260342403543802 1201 28-29 0.05008149572667975 1201 30-31 0.11305747857709747 1201 32-33 0.007670120276820569 1201 34-35 0.1166554498814989 1201 36-37 -0.004489703063867978 1201 38-39 0.21786189820843305 1201 40-41 0.07311785970706808 1201 42-43 0.08690925937538907 1201 44-45 0.051677734084499605 1201 46-47 0.09913272369868054 1201 48-49 0.1649437862019809 1201 50-51 0.08860029375571088 1201 52-53 -0.05361630348409818 1201 54-55 0.019131552139910468 1201 56-57 -0.028387265843029752 1201 58-59 0.16481692237293544 1201 60-61 0.27974248447377903 1201 62-63 0.07393698119793868 1201 64-65 0.04772421738707777 1201 66-67 0.05451871869371416 1201 68-69 0.25973715653576335 1201 70-71 0.09591184991101187 1201 72-73 -0.009611068729000749 1201 74-75 0.13832055488057904 1201 76-77 0.12677063069568817 1201 78-79 0.0682544510060552 1201 80-81 -0.11467388833766279 1201 82-83 -0.07008248222852842 1201 84-85 0.16112065578855805 1201 86-87 0.1276869681874544 1201 88-89 -0.06113145313318569 1201 90-91 0.515384390398907 1202 1 -0.01235966355502427 1202 2 0.004575828761034018 1202 3 0.04421115752391103 1202 4 -0.09563005199267138 1202 5 0.01030765693273139 1202 6 0.05071572654184564 1202 7 0.014562522586388127 1202 8 0.016946180708373504 1202 9 -0.12570071827841645 1202 10-11 0.021855924784524916 1202 12-13 -0.039610320675798505 1202 14-15 -0.09236541237770979 1202 16-17 -0.010456714737841821 1202 18-19 -0.09880203086656536 1202 20-21 0.022364711838726237 1202 22-23 -0.03006614751661374 1202 24-25 0.08345352317918042 1202 26-27 0.09046296795442998 1202 28-29 -0.030535373758588946 1202 30-31 -0.0494374806904645 1202 32-33 -0.01166033511164244 1202 34-35 -0.05698564850033705 1202 36-37 -0.16516503728323073 1202 38-39 -0.1074329295762908 1202 40-41 -0.060434939707874946 1202 42-43 -0.21285487950027715 1202 44-45 -0.14901920334216356 1202 46-47 -0.08218788129425292 1202 48-49 -0.15129482819067874 1202 50-51 -0.219328339179647 1202 52-53 -0.2366354731470821 1202 54-55 -0.19347741483638004 1202 56-57 -0.19643525940671935 1202 58-59 -0.14431206216018921 1202 60-61 0.04146517966810137 1202 62-63 0.06691011576715056 1202 64-65 0.20780513321040672 1202 66-67 0.34099211070824964 1202 68-69 0.04904414975396065 1202 70-71 0.13268966346593913 1202 72-73 0.37807824832152903 1202 74-75 0.3706816763451144 1202 76-77 -0.06655676817888434 1202 78-79 -0.26560381223816165 1202 80-81 0.1684528686263036 1202 82-83 0.5264463636884216 1202 84-85 0.5879546326225338 1202 86-87 0.04882177543322541 1202 88-89 0.12961388848172106 1202 90-91 0.015384390398907044 1203 1 0.04393368720645441 1203 2 0.16218921037854983 1203 3 0.029947537323472773 1203 4 0.0712520339360978 1203 5 -0.22604592473008722 1203 6 0.03409519797811811 1203 7 -0.05805295916622555 1203 8 0.04982505237633461 1203 9 -0.049956386615995996 1203 10-11 0.006965777796686723 1203 12-13 0.005810438821704622 1203 14-15 -0.03137338099839582 1203 16-17 -0.03601187206958656 1203 18-19 -0.03277291872367982 1203 20-21 0.018064975801650007 1203 22-23 -0.00429908466229989 1203 24-25 -0.049811805779810925 1203 26-27 0.08873312695384783 1203 28-29 -0.07480687014133025 1203 30-31 0.024766211330799592 1203 32-33 0.00549606619728138 1203 34-35 0.05822987291312387 1203 36-37 -0.04866916618671269 1203 38-39 -0.04378931612190229 1203 40-41 0.01199085346917883 1203 42-43 -0.02500832466235181 1203 44-45 0.030395814451246395 1203 46-47 0.003881455235642761 1203 48-49 0.07833257351762057 1203 50-51 -0.0170006030316614 1203 52-53 -0.012465537465438103 1203 54-55 0.015076469056694464 1203 56-57 0.10184802271977134 1203 58-59 -0.0779775230001647 1203 60-61 0.2996523067102075 1203 62-63 -0.028438523135058347 1203 64-65 -0.058350372705518794 1203 66-67 -0.03645907761272582 1203 68-69 0.07887920752693134 1203 70-71 -0.1041064091960422 1203 72-73 0.10730174669598114 1203 74-75 -0.027042853640722342 1203 76-77 0.06819412369220856 1203 78-79 0.36720757817540317 1203 80-81 0.08817752381607136 1203 82-83 0.26626323852448763 1203 84-85 0.29152606119396296 1203 86-87 0.4938606425716472 1203 88-89 0.7274399754382443 1203 90-91 0.515384390398907 1204 1 0.03659274775858279 1204 2 0.06004578253607207 1204 3 -0.0474240726268107 1204 4 -0.18999273197779587 1204 5 -0.1261710990667737 1204 6 0.02021787839350253 1204 7 -0.003821197726026071 1204 8 -0.022052809780973348 1204 9 -0.12927828234691674 1204 10-11 -0.06675077340721458 1204 12-13 -0.11975129795295203 1204 14-15 -0.13954675237027203 1204 16-17 -0.0944763382080751 1204 18-19 -0.004191400896495168 1204 20-21 -0.03884351301648792 1204 22-23 0.023880684922332307 1204 24-25 0.016985216500479794 1204 26-27 -0.033299495959994374 1204 28-29 0.023960490789875166 1204 30-31 -0.002256140697909359 1204 32-33 0.016906766225488923 1204 34-35 -0.023265154692843737 1204 36-37 -0.010820882983644253 1204 38-39 -0.0032433547838621735 1204 40-41 -0.09997479997482372 1204 42-43 0.010155477451462502 1204 44-45 -8.259126720560062E-4 1204 46-47 0.160017582374536 1204 48-49 -0.005222631044027537 1204 50-51 -0.12436146847891649 1204 52-53 -0.20108304633409801 1204 54-55 -0.13502490867334416 1204 56-57 -0.1910306275683169 1204 58-59 -0.10195723426893721 1204 60-61 -0.09391958886539697 1204 62-63 -0.04039707015387961 1204 64-65 -0.0841304746717455 1204 66-67 -0.13230465570271832 1204 68-69 -0.019620792111474827 1204 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0.1534981600662917 2401 40-41 0.03894468233828263 2401 42-43 0.11716663193872989 2401 44-45 0.06688630156499187 2401 46-47 -0.07891079427392356 2401 48-49 -0.07925418514663818 2401 50-51 0.03304336151078502 2401 52-53 -0.10649432126655967 2401 54-55 0.04428945387634542 2401 56-57 -0.12328471362145166 2401 58-59 0.02868336217161982 2401 60-61 0.11599132463887685 2401 62-63 0.19099974348771553 2401 64-65 0.12051586256332314 2401 66-67 0.2067304574527995 2401 68-69 0.14800328275304508 2401 70-71 0.09593446912123937 2401 72-73 0.18522044267430715 2401 74-75 0.32893947980081606 2401 76-77 0.33043267223757056 2401 78-79 0.2842798746249997 2401 80-81 0.4237880853814744 2401 82-83 0.2866373253454526 2401 84-85 0.262734174068747 2401 86-87 -0.20845664798093821 2401 88-89 0.18445355009887976 2401 90-91 0.515384390398907 2402 1 0.08005204591734838 2402 2 -0.12296287312790355 2402 3 0.07115500054990775 2402 4 0.0711779721240191 2402 5 0.11459873485118521 2402 6 0.03987995451423387 2402 7 -0.09061899596215994 2402 8 0.06778945024608873 2402 9 0.048343916839051815 2402 10-11 0.0532310456061893 2402 12-13 0.13868394234878423 2402 14-15 0.0707018706519591 2402 16-17 0.014090062111954182 2402 18-19 -0.05144839815818614 2402 20-21 0.03633928708741507 2402 22-23 -0.015787041572430383 2402 24-25 0.06785433900089544 2402 26-27 -0.0010280259064217034 2402 28-29 0.021249536745450825 2402 30-31 0.042004538935735525 2402 32-33 0.03898937228807142 2402 34-35 0.0384826649913137 2402 36-37 0.009644130986814048 2402 38-39 -0.36139432486360334 2402 40-41 0.002501944048418636 2402 42-43 0.07971344841632799 2402 44-45 -0.11629486555925439 2402 46-47 0.08998956306233907 2402 48-49 0.0717242968384042 2402 50-51 0.0471660329986463 2402 52-53 -0.019279932033903435 2402 54-55 0.08248619193520312 2402 56-57 0.1469144442732997 2402 58-59 0.013838964461029946 2402 60-61 0.04807500222939609 2402 62-63 -0.14332099764671824 2402 64-65 -0.06047244440723176 2402 66-67 0.037871750799176596 2402 68-69 -0.03651666204416415 2402 70-71 -1.608715929037885E-4 2402 72-73 0.18507171063195926 2402 74-75 0.04220789561002647 2402 76-77 -0.1867075638463973 2402 78-79 0.04761555434733111 2402 80-81 -0.34156115205249904 2402 82-83 -0.3173072047007679 2402 84-85 -0.9186248638303809 2402 86-87 -0.6772296570845384 2402 88-89 0.35901892280666914 2402 90-91 0.515384390398907 2403 1 -0.037581794817128866 2403 2 0.02071170986506843 2403 3 -0.03881635014668916 2403 4 0.22510153357528395 2403 5 0.05410428785872767 2403 6 -0.046283255148551916 2403 7 0.025618835773165927 2403 8 -0.010147510431004036 2403 9 0.03674162742771614 2403 10-11 -0.02639863930575359 2403 12-13 0.060715139061038315 2403 14-15 0.06414012176893635 2403 16-17 0.07025463775035945 2403 18-19 0.059175726056942324 2403 20-21 0.025285197166418527 2403 22-23 -0.025122354213493736 2403 24-25 0.054122213153824816 2403 26-27 -0.003415859858066028 2403 28-29 -0.014430108498920902 2403 30-31 0.08280433123506725 2403 32-33 -0.04658934041556506 2403 34-35 0.008213330216783277 2403 36-37 0.008830957816606144 2403 38-39 -0.018929996500482105 2403 40-41 0.014248874880664175 2403 42-43 0.040514564777922146 2403 44-45 0.1424161505514121 2403 46-47 -0.004608677290242724 2403 48-49 0.009022478755255747 2403 50-51 0.07379157182319318 2403 52-53 -0.0052699337428165904 2403 54-55 -0.0592032992884981 2403 56-57 -0.25382234006023197 2403 58-59 -0.005195843066601924 2403 60-61 -0.14059236618501103 2403 62-63 -0.014343090594422847 2403 64-65 -0.0587605808217333 2403 66-67 8.782150508395148E-4 2403 68-69 -0.06396126108337086 2403 70-71 -0.01613704868190524 2403 72-73 -0.0422265145926346 2403 74-75 -0.11157195416982546 2403 76-77 -0.009198654494795733 2403 78-79 0.15792708901759767 2403 80-81 -0.23849660004886175 2403 82-83 -0.11470060775788937 2403 84-85 0.16317940784730922 2403 86-87 0.3241927754247911 2403 88-89 -0.3117757108362653 2403 90-91 0.515384390398907 2404 1 0.06759017970516368 2404 2 0.11040039016454273 2404 3 -0.02418767655187537 2404 4 0.0706369006554084 2404 5 0.1227564277626314 2404 6 0.1248482679266516 2404 7 0.13005061586032696 2404 8 0.18046507198044281 2404 9 0.12597260295570578 2404 10-11 0.14487412860128046 2404 12-13 0.14514379030698166 2404 14-15 0.11613967479161857 2404 16-17 0.1323791209328462 2404 18-19 0.03858079924947333 2404 20-21 -0.0032873807752551443 2404 22-23 0.054130299714316266 2404 24-25 0.08840716119271264 2404 26-27 3.691440529820511E-4 2404 28-29 0.010264494249987877 2404 30-31 0.058969712841950184 2404 32-33 -0.01007374449588383 2404 34-35 0.10499397050605808 2404 36-37 0.13157570252437267 2404 38-39 0.06856064341258872 2404 40-41 0.03073453680260485 2404 42-43 0.10956048525247297 2404 44-45 0.1609761622069854 2404 46-47 0.08095746140505611 2404 48-49 0.10322280629407032 2404 50-51 0.035408789757909176 2404 52-53 0.05818575928749681 2404 54-55 0.1498193028204966 2404 56-57 0.09857465736069315 2404 58-59 0.07292740785757701 2404 60-61 0.03289628167429015 2404 62-63 -0.049246754355316114 2404 64-65 0.09070557412385227 2404 66-67 0.18850976792080587 2404 68-69 0.18484339425717167 2404 70-71 0.044599427426511795 2404 72-73 0.20716885823811992 2404 74-75 0.1507978844817046 2404 76-77 0.19635696479473097 2404 78-79 0.24522662560276132 2404 80-81 0.4269652095295271 2404 82-83 0.5018184144248465 2404 84-85 -0.035189342904587306 2404 86-87 0.18779990982083916 2404 88-89 -0.16839335789508425 2404 90-91 0.04479615510479107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 425.0 32 2863.0 33 4993.0 34 6787.0 35 8468.0 36 11835.0 37 17854.0 38 46090.0 39 274354.0 40 293305.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.780939601340357 10.425122004242974 33.83929173819501 29.954646656221655 2 27.583394062624645 16.925628360728993 29.281864884074533 26.209112692571836 3 27.927464635636483 19.37464860529097 21.411725900882328 31.286160858190215 4 26.265958005052585 20.48501840371223 20.76028696297518 32.48873662826001 5 27.269428847725212 20.644092445857105 20.70961116067078 31.376867545746904 6 28.153106891459327 22.94534359843175 23.5090744169659 25.392475093143023 7 26.285148841428217 22.46977068449815 23.31776576684633 27.927314707227296 8 25.278979287390268 23.540109597667115 23.722122686417237 27.458788428525377 9 25.546901354603175 23.75435729439193 23.961258499066695 26.7374828519382 10-11 25.017279249158523 22.443908033913807 23.999265350794996 28.539547366132673 12-13 25.66099687399267 22.299002226436876 23.652630868760166 28.38737003081029 14-15 26.05006109582674 21.93070308927487 23.532013463571143 28.48722235132724 16-17 25.922097198587675 21.64149118795775 23.41386987713367 29.022541736320907 18-19 26.245042991971335 21.87335547276176 23.22481015315187 28.65679138211504 20-21 26.282525094267488 22.007391470572802 23.12405826217981 28.5860251729799 22-23 25.93604054064184 21.60138533850087 23.388831832799838 29.073742288057453 24-25 26.112506278252134 21.972458151232786 23.358996079372098 28.556039491142982 26-27 26.23394828969167 22.0843797086891 23.350225267434798 28.33144673418443 28-29 26.13012286633133 21.690142956738157 23.400976033943792 28.77875814298672 30-31 26.39073907623155 21.899487911456887 23.381178740716475 28.328594271595094 32-33 26.45998380257325 21.838708459418203 23.50614955110319 28.19515818690536 34-35 26.083012712711344 21.54241463651535 23.76178095156294 28.612791699210362 36-37 26.304155651684606 21.843182542679816 23.79959475520035 28.05306705043523 38-39 26.52777248124955 21.817035349374276 23.67811219317951 27.977079976196663 40-41 26.14351708807324 21.521552732091358 23.976175964282728 28.35875421555268 42-43 26.353685828786016 21.82832160973012 23.939170713545067 27.878821847938795 44-45 26.462646940777258 21.87633472771018 23.895891775179805 27.765126556332753 46-47 26.29142305832487 21.480529456268492 24.063491255549874 28.164556229856764 48-49 26.346298946925128 21.799477097313545 24.07996951823266 27.774254437528672 50-51 26.517879586015454 21.77722101397799 24.07200467754451 27.632894722462048 52-53 26.352325231580227 21.619841736675813 24.13732718480169 27.890505846942272 54-55 26.463997412592487 21.838346435384118 24.20579130446262 27.491864847560777 56-57 26.625819352999024 21.86360994227514 24.011566906364358 27.49900379836148 58-59 26.360233165370623 21.5651490278666 24.228994138277983 27.84562366848479 60-61 26.734934113532727 21.880935782123224 24.076846592532487 27.307283511811566 62-63 26.752410580241754 21.824973043805752 24.0733002120382 27.3493161639143 64-65 26.445457343436267 21.57406457475341 24.2954082455189 27.68506983629142 66-67 26.63649690866212 21.674589303266455 24.29996209211281 27.388951695958617 68-69 26.758170743976905 21.64482900062688 24.157385981069723 27.43961427432649 70-71 26.80962852147582 21.570447540599485 23.95504767397848 27.664876263946216 72-73 26.600566421713186 21.814674892002614 24.288651905695566 27.29610678058863 74-75 26.94398457371569 21.637393516139877 24.017938129943612 27.40068378020082 76-77 26.85141202249429 21.430869891665775 23.87260385222415 27.845114233615785 78-79 26.80928968452443 21.664291713457963 23.951796313946794 27.574622288070817 80-81 26.97943032333963 21.46447541977113 23.723456085938707 27.83263817095053 82-83 27.048324168228316 21.042364289883565 23.89941937267672 28.00989216921139 84-85 27.67329842931937 21.04083769633508 23.599999999999998 27.68586387434555 86-87 27.847829421690445 20.754716981132077 23.460653474459274 27.936800122718207 88-89 27.407712930501134 19.85460919777274 24.082285007217983 28.655392864508144 90-91 24.1838432979132 19.638970682371017 27.691716809627447 28.485469210088336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 2.0 7 4.0 8 3.0 9 1.0 10 2.5 11 5.5 12 7.0 13 10.0 14 17.0 15 27.5 16 49.5 17 82.0 18 123.0 19 172.5 20 235.0 21 331.0 22 452.0 23 623.0 24 833.0 25 1087.5 26 1458.0 27 1902.0 28 2346.5 29 2846.5 30 3514.0 31 4294.5 32 5172.5 33 6074.0 34 7180.0 35 8477.5 36 9920.0 37 11460.5 38 13151.0 39 15135.5 40 17139.0 41 19231.0 42 21552.5 43 24007.5 44 26522.0 45 29183.0 46 32222.0 47 35345.0 48 38040.5 49 40588.0 50 43285.0 51 45710.0 52 47328.5 53 48591.5 54 49530.5 55 49994.0 56 50303.0 57 50154.5 58 49470.5 59 48436.0 60 47125.0 61 45588.0 62 43804.0 63 41543.5 64 38644.5 65 35740.0 66 32657.0 67 29500.0 68 26420.5 69 23095.5 70 19931.0 71 17073.5 72 14350.5 73 11611.0 74 9097.0 75 7283.5 76 5820.5 77 4402.0 78 3429.5 79 2640.5 80 1893.5 81 1358.0 82 983.5 83 685.0 84 483.0 85 358.5 86 252.5 87 171.5 88 128.5 89 91.0 90 62.0 91 46.0 92 36.0 93 24.5 94 16.5 95 14.0 96 11.5 97 9.5 98 7.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013643485235799904 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.05690404754412944 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.579533465577416E-4 64-65 0.0 66-67 3.354669028353663E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.436811112788767E-4 80-81 0.001188297644794068 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11819.0 32-33 25530.0 34-35 27833.0 36-37 29771.0 38-39 31635.0 40-41 32576.0 42-43 33343.0 44-45 33962.0 46-47 33488.0 48-49 32730.0 50-51 32336.0 52-53 31493.0 54-55 29684.0 56-57 28532.0 58-59 27103.0 60-61 25219.0 62-63 23773.0 64-65 21940.0 66-67 20181.0 68-69 18648.0 70-71 17322.0 72-73 15571.0 74-75 14108.0 76-77 12482.0 78-79 11287.0 80-81 9764.0 82-83 9013.0 84-85 7730.0 86-87 6898.0 88-89 5975.0 90-91 5239.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.91451818454125 #Duplication Level Percentage of deduplicated Percentage of total 1 99.92063968714653 99.83522571032395 2 0.07464741298916638 0.1491672060507005 3 0.0035009887628130966 0.01049398816217891 4 9.420855380074695E-4 0.0037651209047456255 5 2.6982556347202464E-4 0.0013479745584089847 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0019490693193999866 0.0 0.0 0.0 0.0 2 0.0019490693193999866 0.0 0.0 0.0 0.0 3 0.0019490693193999866 0.0 0.0 0.0 0.0 4 0.0019490693193999866 0.0 0.0 0.0 0.0 5 0.0019490693193999866 0.0 0.0 0.0 0.0 6 0.0019490693193999866 0.0 0.0 0.0 0.0 7 0.0020989977285846007 0.0 0.0 0.0 0.0 8 0.006147064776569188 0.0 0.0 0.0 0.0 9 0.006296993185753803 0.0 0.0 0.0 0.0 10-11 0.006296993185753803 0.0 0.0 0.0 0.0 12-13 0.006296993185753803 0.0 0.0 0.0 0.0 14-15 0.006296993185753803 0.0 0.0 0.0 0.0 16-17 0.006296993185753803 0.0 0.0 0.0 0.0 18-19 0.00637195739034611 0.0 0.0 0.0 0.0 20-21 0.0065218857995307244 0.0 1.4992840918461435E-4 0.0 0.0 22-23 0.0071215994362691815 0.0 1.4992840918461435E-4 0.0 0.0 24-25 0.007571384663823025 0.0 1.4992840918461435E-4 0.0 0.0 26-27 0.007721313073007639 0.0 1.4992840918461435E-4 0.0 0.0 28-29 0.008246062505153789 0.0 1.4992840918461435E-4 0.0 0.0 30-31 0.008695847732707632 0.0 1.4992840918461435E-4 0.0 1.4992840918461435E-4 32-33 0.008920740346484553 0.0 1.4992840918461435E-4 0.0 1.4992840918461435E-4 34-35 0.008995704551076861 0.0 1.4992840918461435E-4 0.0 1.4992840918461435E-4 36-37 0.009670382392407625 0.0 1.4992840918461435E-4 0.0 1.4992840918461435E-4 38-39 0.010195131824553776 0.0 1.4992840918461435E-4 0.0 2.998568183692287E-4 40-41 0.01034506023373839 0.0 1.4992840918461435E-4 0.0 2.998568183692287E-4 42-43 0.010644917052107619 0.0 1.4992840918461435E-4 0.0 3.748210229615359E-4 44-45 0.011019738075069153 0.0 1.4992840918461435E-4 0.0 5.247494321461502E-4 46-47 0.011244630688846076 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 48-49 0.011244630688846076 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 50-51 0.011469523302622998 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 52-53 0.011544487507215305 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 54-55 0.011619451711807612 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 56-57 0.011994272734769149 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 58-59 0.01221916534854607 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 60-61 0.012593986371507606 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 62-63 0.012893843189876833 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 64-65 0.013193700008246063 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 66-67 0.013193700008246063 0.0 1.4992840918461435E-4 0.0 5.997136367384574E-4 68-69 0.013343628417430677 0.0 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