##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-20C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 573895 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.238683034352974 40.0 40.0 40.0 40.0 40.0 2 39.15005358123002 40.0 40.0 40.0 40.0 40.0 3 39.11486595980101 40.0 40.0 40.0 40.0 40.0 4 39.036785474694845 40.0 40.0 40.0 40.0 40.0 5 39.197435070875336 40.0 40.0 40.0 40.0 40.0 6 39.262652575819615 40.0 40.0 40.0 40.0 40.0 7 39.28816246874428 40.0 40.0 40.0 40.0 40.0 8 39.30411660669635 40.0 40.0 40.0 40.0 40.0 9 39.30925343486178 40.0 40.0 40.0 40.0 40.0 10-11 39.315941069359376 40.0 40.0 40.0 40.0 40.0 12-13 39.31615365180042 40.0 40.0 40.0 40.0 40.0 14-15 39.325977748542854 40.0 40.0 40.0 40.0 40.0 16-17 39.46325721604126 40.0 40.0 40.0 40.0 40.0 18-19 39.47591806863625 40.0 40.0 40.0 40.0 40.0 20-21 39.487498584235794 40.0 40.0 40.0 40.0 40.0 22-23 39.48844997778339 40.0 40.0 40.0 40.0 40.0 24-25 39.48991714512236 40.0 40.0 40.0 40.0 40.0 26-27 39.46663065543349 40.0 40.0 40.0 40.0 40.0 28-29 39.46997621516131 40.0 40.0 40.0 40.0 40.0 30-31 39.4630218483212 40.0 40.0 40.0 40.0 40.0 32-33 39.46732336425014 40.0 40.0 40.0 40.0 40.0 34-35 39.46332160987684 40.0 40.0 40.0 40.0 40.0 36-37 39.45773802900938 40.0 40.0 40.0 40.0 40.0 38-39 39.43305414269814 40.0 40.0 40.0 40.0 40.0 40-41 39.423409802961146 40.0 40.0 40.0 40.0 40.0 42-43 39.42532028548389 40.0 40.0 40.0 40.0 40.0 44-45 39.40469627502175 40.0 40.0 40.0 40.0 40.0 46-47 39.38797036461044 40.0 40.0 40.0 40.0 40.0 48-49 39.385306168834035 40.0 40.0 40.0 40.0 40.0 50-51 39.39469028861537 40.0 40.0 40.0 40.0 40.0 52-53 39.378798114025585 40.0 40.0 40.0 40.0 40.0 54-55 39.3633307811951 40.0 40.0 40.0 40.0 40.0 56-57 39.35634283770125 40.0 40.0 40.0 40.0 40.0 58-59 39.33978240681485 40.0 40.0 40.0 40.0 40.0 60-61 39.304140435293746 40.0 40.0 40.0 40.0 40.0 62-63 39.320479404061786 40.0 40.0 40.0 40.0 40.0 64-65 39.32200648146552 40.0 40.0 40.0 40.0 40.0 66-67 39.31523831451276 40.0 40.0 40.0 40.0 40.0 68-69 39.311166339804124 40.0 40.0 40.0 40.0 40.0 70-71 39.310164942856886 40.0 40.0 40.0 40.0 40.0 72-73 39.2875906903111 40.0 40.0 40.0 40.0 40.0 74-75 39.30158181722277 40.0 40.0 40.0 40.0 40.0 76-77 39.29297729563915 40.0 40.0 40.0 40.0 40.0 78-79 39.286185958078946 40.0 40.0 40.0 40.0 40.0 80-81 39.26489412997025 40.0 40.0 40.0 40.0 40.0 82-83 39.233588960017485 40.0 40.0 40.0 40.0 40.0 84-85 39.27117236921485 40.0 40.0 40.0 40.0 40.0 86-87 39.26581236269442 40.0 40.0 40.0 40.0 40.0 88-89 39.32712482018326 40.0 40.0 40.0 40.0 40.0 90-91 39.38279202378669 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08631161974070523 1101 2 0.06682640768499937 1101 3 0.2947363871538471 1101 4 0.055742218289125844 1101 5 -0.1033347753996452 1101 6 -0.08583465587194894 1101 7 0.06418331673620514 1101 8 0.08985074209267907 1101 9 0.007053813898039607 1101 10-11 0.03521926355557525 1101 12-13 0.055300921072415576 1101 14-15 0.07287569381755787 1101 16-17 0.021621047799406767 1101 18-19 -0.010083787813108813 1101 20-21 0.10336022564452207 1101 22-23 0.049998006929293126 1101 24-25 -0.058914899696546286 1101 26-27 -0.012761704160318743 1101 28-29 -0.04023725052450544 1101 30-31 0.00230522533440336 1101 32-33 0.05941849942659161 1101 34-35 0.084149062763764 1101 36-37 -0.019230483031542178 1101 38-39 0.043597176181833674 1101 40-41 0.06489898377823522 1101 42-43 -0.03476718909219301 1101 44-45 -0.006580843271976278 1101 46-47 0.08455000181469785 1101 48-49 0.0859288552073707 1101 50-51 0.045235481443732795 1101 52-53 0.058940763556641684 1101 54-55 0.1375798113478197 1101 56-57 0.1641889349440646 1101 58-59 0.1781345771562357 1101 60-61 0.2293536297458445 1101 62-63 -0.09958210719899796 1101 64-65 0.13612166281468774 1101 66-67 0.07256053773926396 1101 68-69 0.03498354795215164 1101 70-71 -0.026486494413603623 1101 72-73 0.1440259288710095 1101 74-75 -0.08106281878282573 1101 76-77 0.25284721729661896 1101 78-79 0.07892354609218444 1101 80-81 0.13939846890313845 1101 82-83 0.09067598376423547 1101 84-85 0.4101517889765205 1101 86-87 0.4601191258214783 1101 88-89 0.3912588106090169 1101 90-91 0.6946171939896928 1102 1 0.43009249969310304 1102 2 0.05461800997577626 1102 3 0.1074011939715902 1102 4 0.08348927155687136 1102 5 0.13135122496068163 1102 6 0.035884630688549635 1102 7 -0.01119406699139347 1102 8 0.11893426605142565 1102 9 -0.04644432170351109 1102 10-11 0.09440067525733298 1102 12-13 0.06131503918192038 1102 14-15 -0.013369249376978587 1102 16-17 0.10675663296938609 1102 18-19 0.04387184878478223 1102 20-21 0.035824780236183074 1102 22-23 0.0315745837517909 1102 24-25 0.10788188360194084 1102 26-27 0.040977738649324635 1102 28-29 -0.1068729803696229 1102 30-31 0.041554224892188074 1102 32-33 0.013123102925597152 1102 34-35 0.06594591919769499 1102 36-37 0.021049975847539315 1102 38-39 -0.009473955454673444 1102 40-41 0.0752767807141197 1102 42-43 0.05393040259711057 1102 44-45 -0.025847407156305735 1102 46-47 0.09361825491009057 1102 48-49 -0.008480251712676079 1102 50-51 -0.06454645589206365 1102 52-53 0.021039372642668752 1102 54-55 0.22176810999062724 1102 56-57 0.03337241048166106 1102 58-59 0.041818014995783415 1102 60-61 -0.03509142882067806 1102 62-63 -0.19711337748081803 1102 64-65 -0.16122764085315566 1102 66-67 -0.10558853279570712 1102 68-69 -0.1365334603340358 1102 70-71 -0.08826811877001717 1102 72-73 0.04553609715107143 1102 74-75 -0.0011023236198397512 1102 76-77 -0.20809691995530244 1102 78-79 -0.0065319763716473744 1102 80-81 -0.2037733856972892 1102 82-83 0.43019262630300403 1102 84-85 0.4866000645476021 1102 86-87 0.18885314116674579 1102 88-89 -0.002616807616803385 1102 90-91 0.6946171939896928 1103 1 0.3259066137731921 1103 2 0.2266595936961906 1103 3 0.09767962363047644 1103 4 0.16701493229457043 1103 5 0.14079873549417954 1103 6 -0.06737765210016988 1103 7 0.1130025007871609 1103 8 0.07331086091226524 1103 9 0.05066780004810312 1103 10-11 0.01764342858731993 1103 12-13 0.1113341909991874 1103 14-15 0.10215990724172741 1103 16-17 0.04532084011112403 1103 18-19 0.06137822822814343 1103 20-21 0.004715755144616196 1103 22-23 0.05528723096453092 1103 24-25 0.01571578609491553 1103 26-27 0.04085581440420327 1103 28-29 0.09211386152268375 1103 30-31 0.1387548226446782 1103 32-33 -0.047988335939244564 1103 34-35 0.11908993001211599 1103 36-37 -0.08501429441387387 1103 38-39 -0.05615030016641498 1103 40-41 0.061950596156926 1103 42-43 0.12426520985873424 1103 44-45 0.1113165585155258 1103 46-47 0.045962723490809765 1103 48-49 -0.057801907112398965 1103 50-51 0.0692063175815818 1103 52-53 0.07202964387698074 1103 54-55 0.12651811650418665 1103 56-57 0.18338584312187578 1103 58-59 0.13981987574369725 1103 60-61 -0.043105803099564355 1103 62-63 0.2032858569293694 1103 64-65 0.2486139371516103 1103 66-67 -0.03578428189130989 1103 68-69 0.15215314428738225 1103 70-71 0.009363321085757548 1103 72-73 0.09120748590171246 1103 74-75 0.3067052096349627 1103 76-77 -0.07075320639915361 1103 78-79 -0.39602074192234227 1103 80-81 -0.09120536801299295 1103 82-83 -0.18150051121874355 1103 84-85 -0.5601531190817113 1103 86-87 -0.24235077441931452 1103 88-89 0.2536474847006147 1103 90-91 0.6946171939896928 1104 1 0.4004836129089995 1104 2 0.09647196280334214 1104 3 0.22950181888794674 1104 4 0.03153164644491113 1104 5 -0.03813185494007598 1104 6 0.08177634617144491 1104 7 0.08314668229051847 1104 8 -0.04505119717940431 1104 9 -0.06083284053850946 1104 10-11 0.0688851450049981 1104 12-13 0.07058892138467598 1104 14-15 -0.019713128388232803 1104 16-17 0.009514706950426444 1104 18-19 -0.11023643573232533 1104 20-21 -0.06654207757732422 1104 22-23 0.06144290862592783 1104 24-25 -0.0043045000164028124 1104 26-27 0.0290271142477323 1104 28-29 0.011894070992944705 1104 30-31 -0.09951420705164793 1104 32-33 -0.011251138526723992 1104 34-35 -0.07534433544831387 1104 36-37 0.021045811480739474 1104 38-39 -0.04725457015477019 1104 40-41 0.03703216426566769 1104 42-43 0.08840992820186955 1104 44-45 -0.017927267432781946 1104 46-47 -0.09495058259566491 1104 48-49 0.015276890469642979 1104 50-51 0.046267160848493916 1104 52-53 -0.13424538487404902 1104 54-55 0.014856879232468145 1104 56-57 0.036815830688397 1104 58-59 -0.2636893271344718 1104 60-61 -0.21002365446877036 1104 62-63 -0.13118414844377213 1104 64-65 -0.050964902924349076 1104 66-67 -0.020257977785711034 1104 68-69 0.10904042069780928 1104 70-71 -0.15208828068151092 1104 72-73 0.051790889187472544 1104 74-75 0.0442215504784258 1104 76-77 -0.08238257769981061 1104 78-79 -0.13811550911796644 1104 80-81 -0.5890504973413044 1104 82-83 -0.6324292574495516 1104 84-85 -0.25793671150898234 1104 86-87 -1.004169299225957 1104 88-89 -0.7266380549117173 1104 90-91 -2.93871613934364 1201 1 0.3079787955705555 1201 2 0.2045511856722939 1201 3 0.3233773428355775 1201 4 0.20467771609270358 1201 5 0.2052897466014727 1201 6 0.0883160072691993 1201 7 0.11217387790426159 1201 8 0.10809844822475867 1201 9 0.22192208109282774 1201 10-11 0.12748384581351502 1201 12-13 0.09678332333941597 1201 14-15 0.026334667555268254 1201 16-17 0.07925586191973366 1201 18-19 -0.05158409857822477 1201 20-21 0.0849766256150275 1201 22-23 0.08600040877492177 1201 24-25 0.09781711543087113 1201 26-27 0.0395795686071736 1201 28-29 0.08163323943093559 1201 30-31 -0.014105279579901264 1201 32-33 0.08886879228230526 1201 34-35 0.008710855429384878 1201 36-37 0.07953840859096317 1201 38-39 0.05789521341689152 1201 40-41 -0.07719901193898693 1201 42-43 0.17209991858911167 1201 44-45 0.0825208488403888 1201 46-47 0.0385635066696679 1201 48-49 0.16565106295087872 1201 50-51 0.04826269049289067 1201 52-53 0.08319492049453459 1201 54-55 0.055199706078617794 1201 56-57 0.11488707563343326 1201 58-59 0.1441415737042817 1201 60-61 0.07221224503912538 1201 62-63 0.09623351159101645 1201 64-65 0.07858223402298137 1201 66-67 -0.1605187512805486 1201 68-69 0.07177524153794934 1201 70-71 0.20845601058159957 1201 72-73 0.16809725131167852 1201 74-75 0.16810845076091852 1201 76-77 -0.07964809676293783 1201 78-79 0.42059047923181936 1201 80-81 0.473016082816315 1201 82-83 0.265546647155098 1201 84-85 -0.010819621029973803 1201 86-87 -0.3118142882764232 1201 88-89 -0.08812873814152766 1201 90-91 -2.5887161393436386 1202 1 0.16919529984846804 1202 2 0.19415765656398776 1202 3 0.013121057368806532 1202 4 0.24374835276692863 1202 5 -0.027039404532004596 1202 6 0.13336696262121706 1202 7 0.05665736486611195 1202 8 0.07198487667799469 1202 9 0.1229431348514538 1202 10-11 0.0519862159430744 1202 12-13 -0.07775749447694835 1202 14-15 -0.06800330221219753 1202 16-17 -0.03542628764663647 1202 18-19 0.07818901140162637 1202 20-21 -0.025926943693008297 1202 22-23 0.005215491948462159 1202 24-25 0.15235345579510096 1202 26-27 0.06930011719240525 1202 28-29 -0.009578300715268995 1202 30-31 -0.0037506848379891267 1202 32-33 -0.04766988527098448 1202 34-35 -0.05230428161858924 1202 36-37 -0.07572976257230124 1202 38-39 -0.08186580369342522 1202 40-41 -0.03351719154571242 1202 42-43 -0.013749701891924815 1202 44-45 -0.05798393147969705 1202 46-47 0.09184306500657158 1202 48-49 0.036082472045940506 1202 50-51 0.04486176713584911 1202 52-53 -0.04034995018405141 1202 54-55 0.1168622261132981 1202 56-57 -0.037698678728183665 1202 58-59 0.0316627384859558 1202 60-61 -0.1616221169574672 1202 62-63 -0.11286331849210995 1202 64-65 -0.12895098453452647 1202 66-67 -0.3274358561730111 1202 68-69 -0.1580197320297856 1202 70-71 -0.2214207546983573 1202 72-73 -0.24503046018980967 1202 74-75 -0.19364636090152487 1202 76-77 -0.053934755705640214 1202 78-79 -0.21684225432898074 1202 80-81 0.16169401396395955 1202 82-83 -0.06317209755145115 1202 84-85 -0.7147982526401861 1202 86-87 -0.9621077119501962 1202 88-89 -0.5553920989423347 1202 90-91 0.25017274954524993 1203 1 0.28004420302208644 1203 2 0.251546939183811 1203 3 0.0707554724591617 1203 4 0.09757397495556575 1203 5 -0.08191890778048361 1203 6 0.012793842905118424 1203 7 -0.016957801668404215 1203 8 0.03635392926927494 1203 9 0.19857215891309465 1203 10-11 -0.001344238655093477 1203 12-13 -0.06126290766186315 1203 14-15 -0.0038134791695583203 1203 16-17 -0.05816703837425763 1203 18-19 0.010412711751691006 1203 20-21 0.0424329598002231 1203 22-23 0.07980553146193614 1203 24-25 0.03546675130889554 1203 26-27 0.07379358394064894 1203 28-29 0.07240625405345469 1203 30-31 0.03195663076346733 1203 32-33 -0.06679507076578517 1203 34-35 0.09445749843305862 1203 36-37 0.0040951497313912455 1203 38-39 0.05284431249167909 1203 40-41 0.016450925531245275 1203 42-43 0.048859083987096597 1203 44-45 -0.023047360792745053 1203 46-47 -0.003962342942223529 1203 48-49 0.2104304384513469 1203 50-51 0.023419030330359192 1203 52-53 0.0037647071508501995 1203 54-55 0.05024340615808853 1203 56-57 0.009727831689886557 1203 58-59 0.12629148657788392 1203 60-61 -0.020420347270842854 1203 62-63 -0.009067012273675346 1203 64-65 -0.10133829233851799 1203 66-67 -0.3086653637157468 1203 68-69 0.07180899050633371 1203 70-71 -1.7813205694494627E-5 1203 72-73 -0.32458132545995966 1203 74-75 -0.19692402267263986 1203 76-77 -0.07824406591696942 1203 78-79 -0.1387366812807329 1203 80-81 -0.5399458320289767 1203 82-83 -0.5486960733023238 1203 84-85 -0.19658796799783573 1203 86-87 -0.6746139374125306 1203 88-89 0.1384088334088318 1203 90-91 0.6946171939896928 1204 1 0.36233175898864545 1204 2 0.23674704539438096 1204 3 0.011325163990697718 1204 4 -0.03341662174641158 1204 5 -0.024902142991116705 1204 6 0.04037196718979885 1204 7 -0.11828268698845079 1204 8 0.04958630151902099 1204 9 -0.04309351584449672 1204 10-11 -0.03341983128261461 1204 12-13 -0.003868912221598464 1204 14-15 -0.23181827695744772 1204 16-17 0.039828694890708505 1204 18-19 -0.07244618384148538 1204 20-21 -0.04128294523287934 1204 22-23 -0.03830245538993182 1204 24-25 -0.07916558614772384 1204 26-27 -0.1310792838363426 1204 28-29 -0.05450439172512489 1204 30-31 -0.09407503097417447 1204 32-33 -0.01385744622110252 1204 34-35 -0.11256136478956336 1204 36-37 -0.12498975281486224 1204 38-39 -0.1537331983157415 1204 40-41 -0.06399618837075138 1204 42-43 -0.1752287875286953 1204 44-45 -0.11943359877079729 1204 46-47 -0.24043263597400255 1204 48-49 -0.12706956243208367 1204 50-51 -0.16813690083336752 1204 52-53 -0.07239488503333291 1204 54-55 -0.29864732196450916 1204 56-57 -0.019618256565507863 1204 58-59 -0.33234126826604893 1204 60-61 -0.10399230516649993 1204 62-63 -0.003077127801923041 1204 64-65 -0.3514399313793035 1204 66-67 -0.1411260797095224 1204 68-69 -0.04974177090076637 1204 70-71 0.0663692572690664 1204 72-73 -0.15930321003406078 1204 74-75 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46-47 0.15080841641330522 2401 48-49 0.0442235556518753 2401 50-51 0.021079094356011296 2401 52-53 -0.04526031284244425 2401 54-55 0.07113378356378064 2401 56-57 0.1566675668681654 2401 58-59 0.1155558137001691 2401 60-61 0.18154714771238645 2401 62-63 0.118836414381839 2401 64-65 0.24215565612495737 2401 66-67 0.2648854366092337 2401 68-69 -0.04409597805897647 2401 70-71 0.14638625113744297 2401 72-73 0.1869669268404266 2401 74-75 0.1110819048454843 2401 76-77 -0.010673922283579884 2401 78-79 0.2404218632384314 2401 80-81 0.11171603229212934 2401 82-83 0.6792225134872254 2401 84-85 0.24823346039152483 2401 86-87 0.20135161881867703 2401 88-89 0.3524758415934883 2401 90-91 0.6946171939896928 2402 1 0.3697760003468815 2402 2 0.30257911382263813 2402 3 0.3800549888742708 2402 4 0.1991017205727701 2402 5 0.23541216559598865 2402 6 0.08705628992279202 2402 7 -0.10788645846194811 2402 8 0.09778105003484683 2402 9 0.04531115430938826 2402 10-11 0.04463394607304849 2402 12-13 0.0897344460655134 2402 14-15 0.13553024288841442 2402 16-17 0.01440510711299936 2402 18-19 0.07717262302544015 2402 20-21 0.08078379010689929 2402 22-23 0.03296703108251364 2402 24-25 0.04116174928788041 2402 26-27 0.058678858661316724 2402 28-29 0.07864893772501347 2402 30-31 0.058566922938332766 2402 32-33 0.0364619815719891 2402 34-35 0.07807011602378822 2402 36-37 0.05423499622532546 2402 38-39 0.032072890803156895 2402 40-41 0.13537450484027147 2402 42-43 0.0018647795525552624 2402 44-45 0.1051509828911108 2402 46-47 -0.0028909921846178577 2402 48-49 0.09601311042397498 2402 50-51 -0.026483071150586568 2402 52-53 -0.03240950583352742 2402 54-55 0.03063206019750453 2402 56-57 0.12271224545500559 2402 58-59 0.09482119182766269 2402 60-61 0.1872323646970102 2402 62-63 0.12501910557323725 2402 64-65 0.10068680924590012 2402 66-67 0.1645936807668278 2402 68-69 0.09388838397401145 2402 70-71 0.05562766973199729 2402 72-73 -0.06526483533324523 2402 74-75 0.24575451453433317 2402 76-77 0.07373328599940265 2402 78-79 -0.012574036123794485 2402 80-81 0.04364243921853017 2402 82-83 0.09523982950454268 2402 84-85 0.28815596328412596 2402 86-87 -0.2393055480768993 2402 88-89 0.1641270276632767 2402 90-91 0.4446171939896928 2403 1 -0.11169197164879563 2403 2 -0.1714992095258978 2403 3 -0.07246267618147328 2403 4 0.08760396557270411 2403 5 0.14479173744951623 2403 6 -0.01599254446313836 2403 7 0.008911264247636552 2403 8 0.11229408618755343 2403 9 0.20908556834545777 2403 10-11 0.14400491314577835 2403 12-13 0.15131473119584626 2403 14-15 0.08012916449471419 2403 16-17 0.05062924992710549 2403 18-19 0.02828159346432102 2403 20-21 0.0754502691640937 2403 22-23 0.11245788782644439 2403 24-25 0.03621968273341736 2403 26-27 0.03181823265899908 2403 28-29 0.09210450584800611 2403 30-31 0.06645689758602202 2403 32-33 0.03395179172284202 2403 34-35 0.0425355476423519 2403 36-37 0.008928307911531874 2403 38-39 0.10566169082312626 2403 40-41 0.06534478107079167 2403 42-43 -0.004995116070567462 2403 44-45 0.042151846847460206 2403 46-47 0.08138848757920414 2403 48-49 0.06314140205823549 2403 50-51 0.01742406890753756 2403 52-53 0.1401343861007689 2403 54-55 0.133502561219494 2403 56-57 -0.0013291420093111128 2403 58-59 0.10223765529799778 2403 60-61 0.10827094975899598 2403 62-63 0.08229851354577278 2403 64-65 0.24436978555308997 2403 66-67 0.2128904772086031 2403 68-69 0.0816325195465879 2403 70-71 0.07978378314324175 2403 72-73 0.1366908120097321 2403 74-75 0.1913319666744968 2403 76-77 0.019463661178029668 2403 78-79 0.06055029754941188 2403 80-81 0.233144603720703 2403 82-83 0.06336885495064593 2403 84-85 0.3007354028934728 2403 86-87 0.0019505818081313464 2403 88-89 0.295787574378906 2403 90-91 0.3467911070331695 2404 1 -0.38219024200770946 2404 2 -0.2890479403599642 2404 3 -0.41328818829952496 2404 4 -0.12900410796255812 2404 5 -0.10787732924811877 2404 6 -0.0790676105343664 2404 7 0.006884258620587502 2404 8 0.05399937505345065 2404 9 -0.057132060269104556 2404 10-11 0.1685415347326824 2404 12-13 0.06644583552680672 2404 14-15 0.18214526089008842 2404 16-17 0.0628489970440711 2404 18-19 0.035008014567701196 2404 20-21 0.011320174816830786 2404 22-23 -0.01982956690904558 2404 24-25 0.0800241189196953 2404 26-27 -0.011674410647835032 2404 28-29 0.0079380239344502 2404 30-31 0.017888518068374992 2404 32-33 0.03884605413495734 2404 34-35 0.04762245641700957 2404 36-37 0.07436277744439934 2404 38-39 0.03245162634290466 2404 40-41 0.019761370781026244 2404 42-43 0.05816649055108769 2404 44-45 0.014791161150498056 2404 46-47 0.11434860271886294 2404 48-49 -0.03461157351068067 2404 50-51 -0.0066809482406782195 2404 52-53 -0.022907219363517584 2404 54-55 0.08369843346980588 2404 56-57 0.0636354434352171 2404 58-59 0.06577315013730356 2404 60-61 0.05685270095794692 2404 62-63 0.0027466022175914873 2404 64-65 0.1969085655164946 2404 66-67 0.17264447538579475 2404 68-69 0.08081882621664249 2404 70-71 0.2132482976496135 2404 72-73 0.11188485413738647 2404 74-75 -0.0810719527184105 2404 76-77 0.24189966230602522 2404 78-79 0.1598192649795962 2404 80-81 0.009286897499976021 2404 82-83 0.26180322562259306 2404 84-85 0.33762092477898875 2404 86-87 0.5367674330844636 2404 88-89 0.4034692078410913 2404 90-91 -0.47998598061348474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 21.0 31 356.0 32 2375.0 33 4413.0 34 5575.0 35 7169.0 36 10284.0 37 15498.0 38 41852.0 39 249623.0 40 236729.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.705060403877418 10.774943499330007 32.7362355306881 30.78376056610448 2 26.835201717530982 17.218004102138725 29.079876691870833 26.866917488459467 3 27.058923978727705 19.45470576345375 21.740850194810175 31.74552006300837 4 26.360396936721877 20.17546763780831 20.32061614058321 33.14351928488661 5 26.81204749997822 20.886224832068585 20.15176992306955 32.14995774488364 6 27.960863921100547 22.71234285017294 23.054217234860037 26.272575993866475 7 25.702436856916332 22.43459169360249 22.937122644386168 28.92584880509501 8 24.77805173420225 23.8263096907971 23.446972007074464 27.94866656792619 9 25.388093640822802 24.055271434670107 23.537058172662245 27.01957675184485 10-11 24.80497303513709 22.815758980301272 23.48312844684132 28.896139537720316 12-13 25.447337927669693 22.53077653577745 23.109192448095904 28.912693088456948 14-15 26.010855644325183 22.291359917754992 22.902185939936746 28.795598497983082 16-17 25.846539872276285 21.84206170118227 22.68385331811568 29.627545108425757 18-19 26.293572866116627 22.09986147291752 22.46508507653839 29.141480584427466 20-21 26.2108922363847 22.27114716106605 22.328474720985547 29.189485881563698 22-23 25.842183674714015 21.853649186697915 22.575296874863866 29.7288702637242 24-25 26.17421305291038 22.179318516453357 22.62931372463604 29.017154706000227 26-27 26.302285261241167 22.16651129562028 22.643514928689047 28.887688514449504 28-29 26.00135913363943 21.878043893046637 22.71730891539393 29.40328805792 30-31 26.38845568773273 22.14742120918011 22.71102801393291 28.75309508915425 32-33 26.32840885218845 22.173904179589478 22.916343504054787 28.581343464167286 34-35 26.15579544139977 21.887493316403074 23.092316983304443 28.864394258892712 36-37 26.34685040519203 22.060203994203874 23.22470150806571 28.368244092538387 38-39 26.370061735341405 22.170218886963365 23.34220206019318 28.11751731750205 40-41 26.13910074222504 21.863127977590118 23.3834624900321 28.614308790152737 42-43 26.363410726776326 22.080930569389242 23.512435277141158 28.04322342669327 44-45 26.574368373299357 22.13569257219037 23.45691629675809 27.833022757752186 46-47 26.198130083556386 21.84878308269634 23.652235588724533 28.300851245022745 48-49 26.64120121358129 22.090271380996246 23.510238786934032 27.758288618488436 50-51 26.587444318401904 22.112995600807903 23.537752815206265 27.761807265583933 52-53 26.368450535907684 21.83426074616275 23.783396198229827 28.01389251969974 54-55 26.5752361656983 22.154850670549866 23.804073787271484 27.46583937648035 56-57 26.83585389707029 22.060543137782943 23.659695548027724 27.443907417119046 58-59 26.614759963909023 21.795759310537942 23.82257085964967 27.76690986590336 60-61 26.819456270265157 22.18244446164564 23.81548279450594 27.182616473583266 62-63 26.986207028485072 22.009928643850554 23.66278958544564 27.341074742218733 64-65 26.733081047831725 21.724723533423372 23.93324686091759 27.60894855782731 66-67 26.858720079636804 21.98488710577518 23.84187267160375 27.314520142984268 68-69 27.133698158853303 21.98890446117418 23.487384735581454 27.390012644391064 70-71 26.882500019595085 21.811683374744284 23.678703275514764 27.627113330145864 72-73 26.980077579660122 21.960136969482 23.721822803690717 27.337962647167156 74-75 27.419861973407233 22.084254842921474 23.40430067414533 27.09158250952597 76-77 26.720388927221737 21.725231526906498 23.761655472770663 27.7927240731011 78-79 27.086231407738047 21.961416757112065 23.402938074200897 27.549413760948987 80-81 27.56450240998015 21.859937624043095 23.25678102258766 27.318778943389095 82-83 27.90050590219224 21.21536979041195 23.040231269573596 27.84389303782221 84-85 28.334352766957473 21.037890738177254 22.505875156939123 28.12188133792615 86-87 28.545943190769655 20.20063945136926 22.53371020805338 28.7197071498077 88-89 28.2517807370703 19.286156704862186 23.451532982347477 29.01052957572004 90-91 24.09112942317014 20.261754726127 27.72661173048958 27.92050412021328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.5 13 3.0 14 7.0 15 11.0 16 14.5 17 26.5 18 44.5 19 72.0 20 109.5 21 160.5 22 231.5 23 318.5 24 432.5 25 571.5 26 755.5 27 1041.5 28 1410.5 29 1759.5 30 2141.5 31 2625.5 32 3181.0 33 3802.0 34 4604.5 35 5553.5 36 6587.5 37 7613.5 38 8790.5 39 10328.5 40 12007.5 41 13691.0 42 15467.5 43 17448.0 44 19726.0 45 22268.5 46 24894.5 47 27562.5 48 30245.0 49 33132.5 50 35841.0 51 38029.0 52 40091.0 53 42092.5 54 43597.5 55 44387.5 56 44759.5 57 44803.5 58 44527.5 59 43778.5 60 42237.0 61 40382.0 62 38524.0 63 36091.5 64 33246.5 65 30308.5 66 26897.0 67 23747.5 68 21003.5 69 18052.0 70 15242.5 71 12630.0 72 10160.0 73 8002.0 74 6195.5 75 4798.5 76 3673.5 77 2702.5 78 2019.0 79 1480.0 80 1034.0 81 739.5 82 527.5 83 363.0 84 258.0 85 188.5 86 127.0 87 82.5 88 56.5 89 37.0 90 24.5 91 16.5 92 10.0 93 6.5 94 6.5 95 5.5 96 3.5 97 2.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013939832199269901 2 0.00836389931956194 3 5.227437074726213E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6295.0 32-33 14546.0 34-35 17294.0 36-37 19447.0 38-39 21449.0 40-41 23503.0 42-43 24658.0 44-45 25623.0 46-47 26423.0 48-49 26421.0 50-51 26882.0 52-53 26823.0 54-55 25897.0 56-57 25262.0 58-59 24930.0 60-61 23556.0 62-63 22503.0 64-65 21599.0 66-67 20050.0 68-69 18700.0 70-71 17854.0 72-73 16392.0 74-75 15236.0 76-77 13872.0 78-79 12771.0 80-81 11740.0 82-83 10567.0 84-85 9746.0 86-87 8855.0 88-89 8047.0 90-91 6954.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.66081672641639 #Duplication Level Percentage of deduplicated Percentage of total 1 99.69051651273114 99.35238295537084 2 0.287268745643861 0.5725887562168072 3 0.016397731144578844 0.04902633834986767 4 0.003919582613119837 0.015625152178007373 5 0.001418486993571966 0.007068378614759055 6 2.559239621028531E-4 0.0015303354649818467 7 0.0 0.0 8 2.2301691165072378E-4 0.0017780838047131344 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.712395124543689E-4 0.0 0.0 0.0 1.7424790249087377E-4 2 8.712395124543689E-4 0.0 0.0 0.0 3.4849580498174754E-4 3 8.712395124543689E-4 0.0 0.0 0.0 3.4849580498174754E-4 4 8.712395124543689E-4 0.0 0.0 0.0 3.4849580498174754E-4 5 8.712395124543689E-4 0.0 0.0 0.0 3.4849580498174754E-4 6 8.712395124543689E-4 0.0 0.0 0.0 3.4849580498174754E-4 7 0.0012197353174361165 0.0 0.0 0.0 3.4849580498174754E-4 8 0.003833453854799223 0.0 0.0 0.0 3.4849580498174754E-4 9 0.003833453854799223 0.0 0.0 0.0 3.4849580498174754E-4 10-11 0.003833453854799223 0.0 0.0 0.0 3.4849580498174754E-4 12-13 0.003833453854799223 0.0 0.0 0.0 3.4849580498174754E-4 14-15 0.003833453854799223 0.0 0.0 0.0 3.4849580498174754E-4 16-17 0.00392057780604466 0.0 0.0 0.0 3.4849580498174754E-4 18-19 0.00418194965978097 0.0 0.0 0.0 5.227437074726213E-4 20-21 0.00418194965978097 0.0 0.0 0.0 5.227437074726213E-4 22-23 0.004269073611026407 0.0 0.0 0.0 5.227437074726213E-4 24-25 0.0043561975622718446 1.7424790249087377E-4 0.0 0.0 5.227437074726213E-4 26-27 0.0043561975622718446 1.7424790249087377E-4 0.0 0.0 6.969916099634951E-4 28-29 0.004878941269744466 1.7424790249087377E-4 0.0 0.0 6.969916099634951E-4 30-31 0.004966065220989902 1.7424790249087377E-4 0.0 0.0 8.712395124543689E-4 32-33 0.005053189172235339 1.7424790249087377E-4 0.0 0.0 8.712395124543689E-4 34-35 0.005488808928462523 1.7424790249087377E-4 0.0 0.0 0.0010454874149452426 36-37 0.005663056830953398 1.7424790249087377E-4 0.0 0.0 0.0010454874149452426 38-39 0.005924428684689708 1.7424790249087377E-4 0.0 0.0 0.0012197353174361165 40-41 0.006098676587180582 1.7424790249087377E-4 0.0 0.0 0.0012197353174361165 42-43 0.006098676587180582 1.7424790249087377E-4 0.0 0.0 0.0012197353174361165 44-45 0.006185800538426019 1.7424790249087377E-4 0.0 0.0 0.0013939832199269901 46-47 0.006272924489671455 1.7424790249087377E-4 0.0 0.0 0.0013939832199269901 48-49 0.006272924489671455 1.7424790249087377E-4 0.0 0.0 0.0013939832199269901 50-51 0.00670854424589864 1.7424790249087377E-4 8.712395124543688E-5 0.0 0.0013939832199269901 52-53 0.006969916099634951 1.7424790249087377E-4 1.7424790249087377E-4 0.0 0.0013939832199269901 54-55 0.007057040050880387 1.7424790249087377E-4 1.7424790249087377E-4 0.0 0.0013939832199269901 56-57 0.007231287953371262 1.7424790249087377E-4 1.7424790249087377E-4 0.0 0.0013939832199269901 58-59 0.007318411904616698 1.7424790249087377E-4 1.7424790249087377E-4 0.0 0.0013939832199269901 60-61 0.007492659807107572 1.7424790249087377E-4 1.7424790249087377E-4 0.0 0.0013939832199269901 62-63 0.007666907709598446 1.7424790249087377E-4 1.7424790249087377E-4 0.0 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