##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	AM-AL-20B_S106_R2_001.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1186085
Sequences flagged as poor quality	0
Sequence length	61
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	39.39700611676229	40.0	40.0	40.0	40.0	40.0
2	39.25529114692455	40.0	40.0	40.0	40.0	40.0
3	39.124706913922694	40.0	40.0	40.0	40.0	40.0
4	39.04665517226843	40.0	40.0	40.0	40.0	40.0
5	39.08885788118052	40.0	40.0	40.0	40.0	40.0
6	39.154192153176204	40.0	40.0	40.0	40.0	40.0
7	39.16704452041802	40.0	40.0	40.0	40.0	40.0
8	38.86963919112036	40.0	40.0	40.0	40.0	40.0
9	39.183933697837844	40.0	40.0	40.0	40.0	40.0
10	39.185441178330386	40.0	40.0	40.0	40.0	40.0
11	39.182841027413716	40.0	40.0	40.0	40.0	40.0
12	39.18460818575397	40.0	40.0	40.0	40.0	40.0
13	39.17361740516068	40.0	40.0	40.0	40.0	40.0
14	39.199392960875485	40.0	40.0	40.0	40.0	40.0
15	39.194678290341756	40.0	40.0	40.0	40.0	40.0
16	39.18714088787903	40.0	40.0	40.0	40.0	40.0
17	39.18984221198312	40.0	40.0	40.0	40.0	40.0
18	39.183191761130104	40.0	40.0	40.0	40.0	40.0
19	39.20008093854994	40.0	40.0	40.0	40.0	40.0
20	39.10079968973556	40.0	40.0	40.0	40.0	40.0
21	39.145083193868906	40.0	40.0	40.0	40.0	40.0
22	39.16252207894038	40.0	40.0	40.0	40.0	40.0
23	39.193477701851045	40.0	40.0	40.0	40.0	40.0
24	39.203662469384575	40.0	40.0	40.0	40.0	40.0
25	39.14041236504972	40.0	40.0	40.0	40.0	40.0
26	39.1892891318919	40.0	40.0	40.0	40.0	40.0
27	39.19594295518449	40.0	40.0	40.0	40.0	40.0
28	39.12561747260947	40.0	40.0	40.0	40.0	40.0
29	39.16947942179523	40.0	40.0	40.0	40.0	40.0
30	39.1212569082317	40.0	40.0	40.0	40.0	40.0
31	39.10311992816704	40.0	40.0	40.0	40.0	40.0
32	39.1106505857506	40.0	40.0	40.0	40.0	40.0
33	39.09880826416319	40.0	40.0	40.0	40.0	40.0
34	39.08863867260778	40.0	40.0	40.0	40.0	40.0
35	39.125330815245114	40.0	40.0	40.0	40.0	40.0
36	39.06876488615909	40.0	40.0	40.0	40.0	40.0
37	39.117129042185006	40.0	40.0	40.0	40.0	40.0
38	39.05192292289338	40.0	40.0	40.0	40.0	40.0
39	39.04414607722043	40.0	40.0	40.0	40.0	40.0
40	39.00791595880565	40.0	40.0	40.0	40.0	40.0
41	39.00476947267692	40.0	40.0	40.0	40.0	40.0
42	38.94133388416513	40.0	40.0	40.0	40.0	40.0
43	38.92181757631199	40.0	40.0	40.0	40.0	40.0
44	38.83664998714257	40.0	40.0	40.0	40.0	40.0
45	38.81555537756569	40.0	40.0	40.0	40.0	40.0
46	38.70226164229376	40.0	40.0	40.0	40.0	40.0
47	38.60886192810802	40.0	40.0	40.0	40.0	40.0
48	38.54394246618075	40.0	40.0	40.0	40.0	40.0
49	38.42407247372659	40.0	40.0	40.0	40.0	40.0
50	38.31654223769797	40.0	40.0	40.0	40.0	40.0
51	38.16148083821986	40.0	40.0	40.0	40.0	40.0
52	38.05163710863893	40.0	40.0	40.0	40.0	40.0
53	37.821980718076695	40.0	40.0	40.0	24.0	40.0
54	37.675782089816494	40.0	40.0	40.0	24.0	40.0
55	37.52755999780791	40.0	40.0	40.0	24.0	40.0
56	37.25814254458997	40.0	40.0	40.0	24.0	40.0
57	37.100742358262686	40.0	40.0	40.0	24.0	40.0
58	36.89923066222067	40.0	40.0	40.0	24.0	40.0
59	36.62816071360822	40.0	40.0	40.0	24.0	40.0
60	36.365235206583	40.0	40.0	40.0	24.0	40.0
61	35.96919445065067	40.0	40.0	40.0	24.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.06130140003291018
1101	2	0.04011607168171594
1101	3	0.12193533627047515
1101	4	0.14118756593644122
1101	5	0.15743044611284063
1101	6	0.05779818643974011
1101	7	0.08690744774549586
1101	8	0.3955020320775091
1101	9	0.13654834665177873
1101	10	0.049684998741405195
1101	11	0.1377365983236487
1101	12	0.018588472773330977
1101	13	2.3857619147094056E-4
1101	14	0.11417396013663961
1101	15	0.060516289691918246
1101	16	0.060789484741484046
1101	17	0.17565935519789377
1101	18	0.07815217312499811
1101	19	0.13458192391158974
1101	20	0.10904461915986019
1101	21	0.08005822120918538
1101	22	0.08909386357957771
1101	23	0.049960619629906944
1101	24	0.09992619163804761
1101	25	0.10106114815654621
1101	26	0.10005704166515983
1101	27	0.017640551322102738
1101	28	0.1780489859625547
1101	29	0.10603522876193949
1101	30	0.13928549532409562
1101	31	0.15229152409899882
1101	32	0.08426176908504601
1101	33	0.16176579856968232
1101	34	0.07936009598142846
1101	35	0.14229199489550837
1101	36	0.08383918411768576
1101	37	0.10293562506451792
1101	38	0.19051093050308054
1101	39	0.11825319490473163
1101	40	0.09875681191886088
1101	41	0.04350255428149552
1101	42	0.21850397268531196
1101	43	0.23680660011619636
1101	44	0.1881826458847229
1101	45	0.1686707290715077
1101	46	0.11776274559834832
1101	47	0.1952946808418119
1101	48	0.26297339221930116
1101	49	0.17847158400553553
1101	50	0.21733844492928256
1101	51	0.21252241518239856
1101	52	0.2637028634578087
1101	53	0.23817951699381723
1101	54	0.3577522567554965
1101	55	0.28562488332395475
1101	56	0.24889858381594365
1101	57	0.18791823990977008
1101	58	0.1446720887717845
1101	59	0.04808346592152901
1101	60	0.23487647612809326
1101	61	0.31391970695067073
1102	1	0.10081943859126596
1102	2	0.0187401953712083
1102	3	-0.02836666423910117
1102	4	0.013797103193589066
1102	5	0.04713393425814871
1102	6	-0.04204719616017627
1102	7	0.02945528466494096
1102	8	0.3094764959999807
1102	9	-0.0355212607793689
1102	10	-0.05738471519842392
1102	11	-0.01225528211883642
1102	12	-0.0021953812859862865
1102	13	0.004559508687378866
1102	14	0.03379974674471242
1102	15	0.01677359141495316
1102	16	-0.06528359016952123
1102	17	-0.09000454381085632
1102	18	-0.0668086247660753
1102	19	-0.06586657444056954
1102	20	-0.034085612733044
1102	21	-0.06293009133097627
1102	22	-0.0421837508695333
1102	23	-0.04863045040604419
1102	24	-0.09858661526946833
1102	25	-0.09354873822774579
1102	26	-0.09850307169327266
1102	27	-0.12755152255120805
1102	28	-0.016224498993025804
1102	29	-0.06527415570367623
1102	30	-0.01732818166247796
1102	31	-0.07598589310156711
1102	32	-0.09481334276981102
1102	33	0.02417125137126419
1102	34	-0.12294718625189915
1102	35	-0.06389718277175405
1102	36	0.07437180772515006
1102	37	-0.08011073885376874
1102	38	-0.01147571911851486
1102	39	-0.1220765780879276
1102	40	-0.1397739327095806
1102	41	-0.02120215802197123
1102	42	0.014989684338942766
1102	43	-0.058466157537282015
1102	44	-0.018192437835246267
1102	45	-0.004010542470560097
1102	46	-0.09046060945814105
1102	47	-0.16077931530008271
1102	48	-0.04979998581263345
1102	49	-0.07631791750343098
1102	50	-0.1380245712193684
1102	51	-0.23228686374280016
1102	52	-0.2911868668649902
1102	53	-0.15330045287391414
1102	54	-0.1424592390440509
1102	55	-0.15663952985888585
1102	56	-0.15062431264462361
1102	57	-0.09033055827123349
1102	58	-0.024097409577883866
1102	59	-0.15205380577989303
1102	60	-0.10916646595691759
1102	61	-0.3269808972163375
1103	1	0.027288281287368932
1103	2	-0.035600621558586454
1103	3	0.0012490096555026753
1103	4	0.0944474924387606
1103	5	0.07094802332402139
1103	6	0.0897705861980782
1103	7	-0.011837458974227388
1103	8	0.3307634303504514
1103	9	0.08316827097370094
1103	10	0.022039332061112304
1103	11	0.05146085937214906
1103	12	0.08174855508347179
1103	13	0.022070976040659218
1103	14	0.11024810285297093
1103	15	0.06025623145704628
1103	16	-0.06980176730832
1103	17	0.017865330404916335
1103	18	-0.033448014026085104
1103	19	0.040669103608422574
1103	20	0.08119681114416011
1103	21	0.05201735686192421
1103	22	0.08771862112353546
1103	23	0.01500266048517318
1103	24	0.012502335326473712
1103	25	0.02897732082612947
1103	26	-0.07965455488799478
1103	27	0.07125797272546208
1103	28	0.02798629943607267
1103	29	0.1146637111574691
1103	30	0.017615717043611312
1103	31	0.15213026528086715
1103	32	0.08832163017533645
1103	33	0.0695473314823829
1103	34	0.05724833226420856
1103	35	0.049777129557973865
1103	36	0.09327282497839917
1103	37	0.053348538488975805
1103	38	-0.024397106545571035
1103	39	0.005952326832620258
1103	40	0.023473927611838974
1103	41	0.073160549312
1103	42	-0.024259821757389943
1103	43	0.019950965369694984
1103	44	0.13443235630023054
1103	45	-0.03475954125277525
1103	46	-0.0037310107327570563
1103	47	0.034604811075347186
1103	48	0.11999473743581746
1103	49	0.0937096338186123
1103	50	-0.1772198648966068
1103	51	-0.012112254100749453
1103	52	-0.27082696488103863
1103	53	-0.008893976144733529
1103	54	0.14029019800278064
1103	55	-0.04809342704702857
1103	56	0.06888491088516702
1103	57	-0.06365005887784037
1103	58	-0.06819750054061302
1103	59	-0.06051185620999178
1103	60	-0.29233311036795584
1103	61	-0.1549880030378361
1104	1	0.07687120340513331
1104	2	0.029020838145704886
1104	3	-0.02602070108778065
1104	4	-0.08158293694428664
1104	5	-0.02631911483189242
1104	6	0.08015696377877646
1104	7	0.015755248082058415
1104	8	0.40318774107347366
1104	9	0.03294675526441182
1104	10	0.004802996654909464
1104	11	-0.011124727536341084
1104	12	0.005171109389536355
1104	13	-0.0395706648147609
1104	14	0.04841237963149325
1104	15	-0.023257524486375303
1104	16	-0.059832137499086
1104	17	-0.010910368433890483
1104	18	-0.010870714036215645
1104	19	0.1319979474352948
1104	20	0.017786516469080027
1104	21	-0.027961652646403934
1104	22	-0.017822060475523926
1104	23	-0.026455552613853683
1104	24	-0.0710279261554092
1104	25	-0.049005941419558496
1104	26	0.002706659145587764
1104	27	0.05893175043342325
1104	28	0.00815660244360572
1104	29	0.0685580506684289
1104	30	0.03551255856653768
1104	31	0.018038701659989442
1104	32	-0.027022772113582505
1104	33	0.021197202340772492
1104	34	-0.051923567796464454
1104	35	0.006960055318337766
1104	36	-0.02121898087548857
1104	37	0.00438941189377573
1104	38	-0.024765350800215913
1104	39	0.05110208898211965
1104	40	-0.02638987323802411
1104	41	0.01608895056277504
1104	42	-0.08113989934480514
1104	43	-0.034443605787956244
1104	44	0.055420059104918096
1104	45	-0.07981482342349722
1104	46	-0.00801220591587537
1104	47	-0.12095562025421458
1104	48	-0.002760651622487842
1104	49	0.05527591835360113
1104	50	-0.057500356602290026
1104	51	-0.1615929557113489
1104	52	0.06399528185285419
1104	53	0.0011374071446041967
1104	54	-0.046578394006495216
1104	55	-0.07505408581153006
1104	56	-0.18298362990002204
1104	57	-0.18661404379291469
1104	58	-0.0779400369068668
1104	59	-0.013420416665226753
1104	60	-0.3643262850432265
1104	61	-0.4101405768204387
1201	1	-0.11586194747929568
1201	2	-0.024956015023569478
1201	3	-0.03293028137310472
1201	4	-0.13404775660443136
1201	5	-0.20297212045913682
1201	6	-0.06137735262109345
1201	7	-0.07845577216788513
1201	8	0.2806343357008103
1201	9	-0.05487975406062873
1201	10	-0.12309863499466189
1201	11	-0.10959726059007835
1201	12	0.05534003209960048
1201	13	-0.04010543202615935
1201	14	-0.011966440594449068
1201	15	-0.08377211462610035
1201	16	-0.060378542131665824
1201	17	0.0244945459382393
1201	18	-0.07665594764497285
1201	19	-0.08205634445949528
1201	20	0.05832242525607967
1201	21	0.032616666228356905
1201	22	-0.08997240930722228
1201	23	-0.03427340591418471
1201	24	-0.04731113887056182
1201	25	0.0051917456706931375
1201	26	0.0012519814979938815
1201	27	-0.08256765370290253
1201	28	-0.018696469849437847
1201	29	-0.1467575160171819
1201	30	-0.09670489385234049
1201	31	0.08493946521335971
1201	32	7.984227891171258E-4
1201	33	-0.01623788786447733
1201	34	-0.09159027205384973
1201	35	0.0896436557258724
1201	36	0.011816609960540347
1201	37	-0.04670551704452919
1201	38	-0.018104312581542104
1201	39	-0.003220007682180892
1201	40	-0.052482079851017716
1201	41	-0.11842412172472905
1201	42	-0.04846506856537758
1201	43	-0.07969710345646064
1201	44	0.0227939946618676
1201	45	-0.0030845951942737315
1201	46	0.05708032405117791
1201	47	0.05856600969407566
1201	48	-0.04967691511254202
1201	49	-0.03813683021963499
1201	50	0.025692519522628743
1201	51	0.13711637115527253
1201	52	0.013035291583953779
1201	53	0.19417008308370498
1201	54	0.01228008026663474
1201	55	0.13732275494449198
1201	56	0.2773739104700539
1201	57	0.03215368076151748
1201	58	0.08690652785108455
1201	59	0.06780047073247175
1201	60	0.30687361897575727
1201	61	0.3224192928626053
1202	1	-0.07389312575995888
1202	2	-0.055782488109166195
1202	3	0.01889756222026051
1202	4	0.02882317262449874
1202	5	0.029725744671573295
1202	6	0.026661442083344866
1202	7	0.023375000819235936
1202	8	0.20825139862054698
1202	9	0.02606289962251651
1202	10	0.03282556631103262
1202	11	-0.04742907750661374
1202	12	-0.129689448253977
1202	13	-0.030499823736846565
1202	14	-0.01561346574602851
1202	15	0.04593303137965421
1202	16	-0.01997589485674922
1202	17	0.028669702941130026
1202	18	0.030640597548561743
1202	19	0.016913994657784315
1202	20	-0.02331599448596222
1202	21	0.044931333016123176
1202	22	-0.13194207200084662
1202	23	0.03534777161240754
1202	24	0.022206318494141897
1202	25	0.0011713279363405604
1202	26	-0.044835091297578344
1202	27	-0.03903490013724564
1202	28	-0.0038947617746032392
1202	29	0.02345231547680271
1202	30	-0.007336518802482317
1202	31	0.0068667764419600985
1202	32	0.001662944354642093
1202	33	0.08015822896479108
1202	34	0.1147890224864625
1202	35	0.05203836924938088
1202	36	-0.020544921520915693
1202	37	-0.14177709937408878
1202	38	-0.011518923276319981
1202	39	-0.0199310077099355
1202	40	-0.023274482265058793
1202	41	0.041212981051579334
1202	42	0.035360725293323014
1202	43	0.04405674785536462
1202	44	0.1391301724505567
1202	45	0.18012473648066418
1202	46	0.11648955082858237
1202	47	-0.08323963498138909
1202	48	0.032501690445563725
1202	49	0.11630389244638906
1202	50	0.01999772198578853
1202	51	0.049999284267769895
1202	52	0.0010780388675399877
1202	53	0.021396961967433015
1202	54	0.0736199036788534
1202	55	0.007947831508190006
1202	56	0.11264050352343702
1202	57	0.12423840275364029
1202	58	0.139189618857678
1202	59	0.048063925550145825
1202	60	-0.01976303612190833
1202	61	0.07293165644606603
1203	1	-0.009419489524063351
1203	2	0.011396775024699934
1203	3	-0.10761018262607536
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2403	10	0.13525944528122125
2403	11	0.1474418015069361
2403	12	0.060173975816582015
2403	13	0.13223645159074948
2403	14	0.17882695989336383
2403	15	0.147431876324994
2403	16	0.03221800778838002
2403	17	0.06976855538506754
2403	18	0.11783659879699115
2403	19	0.01385617294933894
2403	20	0.021448866780836795
2403	21	0.1642384690830312
2403	22	0.08668760086174387
2403	23	0.1335956060011938
2403	24	0.03857241908886522
2403	25	0.07469442423553829
2403	26	0.07719500580772376
2403	27	-0.0019380620090316825
2403	28	0.06731235799289692
2403	29	0.21572931222307545
2403	30	0.09586439144613479
2403	31	0.23067992075360166
2403	32	0.1672747283592102
2403	33	0.08625498280542132
2403	34	0.20632361595487936
2403	35	0.09559029075575154
2403	36	0.11968871677890291
2403	37	0.20225732201314628
2403	38	0.21581140289371348
2403	39	0.23954565657979288
2403	40	0.18836032326746732
2403	41	0.11929390313765964
2403	42	0.13275623910482182
2403	43	0.11335115492372694
2403	44	0.15673697475869375
2403	45	0.21011737670106356
2403	46	0.08446284669186355
2403	47	0.12005141575271949
2403	48	0.0879946862050005
2403	49	0.13794104343463687
2403	50	0.1909824456134004
2403	51	0.10758215405519422
2403	52	0.10938256917464884
2403	53	0.012661391564478208
2403	54	0.21021386408028775
2403	55	0.19273625609034184
2403	56	0.3224710683174763
2403	57	0.16793886501877608
2403	58	0.19476389703616803
2403	59	0.14806162159425185
2403	60	0.052693657735730426
2403	61	0.12485394988103593
2404	1	-0.16781011658650868
2404	2	0.02479562400750268
2404	3	-0.01440731689559982
2404	4	-0.010350636800133373
2404	5	-0.0017250920168692119
2404	6	-0.01618959556989097
2404	7	0.09135567487341945
2404	8	0.40221262462483054
2404	9	0.01081884464747418
2404	10	0.1441624450707195
2404	11	0.10628893656974014
2404	12	0.024909302096745023
2404	13	0.03975013477201372
2404	14	0.044228993544713546
2404	15	0.040661838001838646
2404	16	0.08213749751412536
2404	17	0.05159264975775102
2404	18	-0.05857560667646311
2404	19	-0.011139968291786317
2404	20	-0.011837329528752605
2404	21	0.017008731132499122
2404	22	0.024997373853821614
2404	23	0.04656547767350361
2404	24	-0.01227287565420454
2404	25	0.0021111326094853666
2404	26	0.003929839176933569
2404	27	0.029816812394493297
2404	28	-0.04881407500307944
2404	29	-0.05593384924953426
2404	30	-0.001309778890998814
2404	31	0.017717729459882037
2404	32	0.05081226023018104
2404	33	0.0558026530732505
2404	34	-0.024015543324438227
2404	35	0.03472603869909818
2404	36	-0.00562089384075648
2404	37	0.06987819772783155
2404	38	0.12144227914468786
2404	39	0.016478048004572088
2404	40	0.05668208923756879
2404	41	0.14536202913568275
2404	42	0.04724317124519217
2404	43	0.003573735860733507
2404	44	0.01563490133872847
2404	45	0.03423284443388752
2404	46	0.04621931510820332
2404	47	0.09944393494512127
2404	48	0.05069211241654159
2404	49	-0.024112270871562203
2404	50	0.028333419157092976
2404	51	0.06635946749441501
2404	52	0.003915966862720666
2404	53	0.17334204246245122
2404	54	0.05424899535014305
2404	55	-0.05938309015939325
2404	56	0.04442531910991221
2404	57	0.06179781553441188
2404	58	0.08491457655654955
2404	59	0.019768399298101258
2404	60	0.16170125625961163
2404	61	0.18326701728014427
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	12.0
14	51.0
15	169.0
16	541.0
17	879.0
18	1154.0
19	1201.0
20	1214.0
21	1155.0
22	1311.0
23	1624.0
24	2106.0
25	2628.0
26	3445.0
27	3388.0
28	3621.0
29	4049.0
30	4927.0
31	6310.0
32	9352.0
33	11928.0
34	16882.0
35	25141.0
36	39186.0
37	54868.0
38	118827.0
39	431057.0
40	439059.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.053591482559415	32.82769179923176	18.57768926989994	19.54102744830889
2	26.90945421280937	18.988605369766923	23.112002934022435	30.989937483401274
3	29.265777747800538	19.366065669829734	19.23858745368165	32.12956912868808
4	29.808993453251663	21.234903063439802	19.747825830357858	29.208277652950677
5	26.8773317258038	22.045215983677394	21.298473549534812	29.77897874098399
6	25.39860128068393	21.781575519461086	23.27607211962043	29.543751080234554
7	27.18607856941113	24.316132486288925	23.021284309303297	25.47650463499665
8	26.20503589540379	25.116918264711213	24.304497569735727	24.37354827014927
9	25.457197418397502	24.726979938200046	24.450271270608766	25.36555137279369
10	27.33581488679142	25.085133021663708	23.095393669087798	24.483658422457076
11	26.587554854837553	25.245829767681066	23.17607928605454	24.990536091426836
12	26.59134884936577	24.10898038504829	23.232736271009244	26.066934494576692
13	27.934760156312578	24.83354902894818	22.019501131875035	25.21218968286421
14	27.589759587213397	24.818710294793377	22.264424556418806	25.327105561574427
15	27.138948726271728	24.05038424733472	22.580675078093055	26.2299919483005
16	28.26947478469081	24.532137241428735	21.655699212113802	25.54268876176665
17	27.71968282205744	24.62454208593819	21.971190934882408	25.68458415712196
18	27.090638529279097	23.887916970537525	22.240058680448705	26.78138581973467
19	28.564816180965106	24.486440685111102	21.389866662170082	25.55887647175371
20	27.6405991138915	24.534919504082758	22.08399903885472	25.740482343171024
21	27.463630346897567	23.988837224988092	22.118482233566734	26.42905019454761
22	28.55545766112884	24.522947343571495	21.3608636817766	25.56073131352306
23	27.858964576737755	24.67184055105663	21.902140234468863	25.567054637736756
24	27.49895665150474	23.700325018864586	22.051286374922537	26.749431954708136
25	28.436073299974286	24.85353073346345	21.26255706800103	25.44783889856123
26	27.691522951559122	25.12518074168377	21.72474991252735	25.45854639422976
27	27.394663957473536	24.350362748032392	21.889072031093892	26.365901263400175
28	28.525527259850687	25.009843308025985	21.254125969049436	25.2105034630739
29	27.902974913265073	25.241951462163335	21.63074315921709	25.224330465354505
30	27.71799660226712	24.53947229751662	21.749200099486966	25.99333100072929
31	28.50141431684913	25.44708009965559	21.196541563210054	24.854964020285227
32	28.21340797666272	25.426002352276605	21.475020761581167	24.885568909479506
33	27.905274837195343	24.919820181369953	21.70630410662314	25.468600874811564
34	28.98847890328265	25.699338580287247	20.87995379757775	24.432228718852357
35	28.47755430681612	25.704397239658206	21.414822715066794	24.403225738458882
36	28.188536234755517	25.273652394221322	21.263062933938123	25.27474843708503
37	29.241833426778012	26.12983049275558	20.6261777191348	24.002158361331606
38	28.980722292247187	26.102345110173385	20.950353473823547	23.966579123755885
39	28.903409114861077	25.838283091009494	20.738479957170018	24.51982783695941
40	29.69475206245758	26.202000699781212	20.434370217986064	23.66887701977514
41	29.340392973522135	26.336476728059115	20.57550681443573	23.74762348398302
42	29.4963683041266	25.750346728944386	20.698347926160434	24.05493704076858
43	30.47530320339605	26.34701560174861	20.185568487924556	22.992112706930783
44	30.252553569094964	26.396421841604944	20.296521750127518	23.05450283917257
45	30.275486158243297	25.97571000392046	20.450642238962637	23.298161598873605
46	31.198438560474166	26.509651500524832	19.779948317363427	22.51196162163757
47	31.07247794213737	26.879102256583636	19.739226109427232	22.309193691851764
48	31.14372072827833	26.499365559803895	19.796641893287582	22.5602718186302
49	32.028564563248	26.676840192734925	19.44852181757631	21.84607342644077
50	31.801009202544506	26.840909378332917	19.57718038757762	21.780901031544957
51	31.891474894295097	26.484273892680545	19.723207021419206	21.901044191605155
52	32.74874903569306	27.089458175425875	19.192975208353534	20.968817580527535
53	32.61267109861435	27.201085925544966	19.351058313695898	20.835184662144787
54	32.713169798117335	27.1282412306032	19.396080382097406	20.762508589182058
55	33.139867716057445	27.639081516080218	19.2711314956348	19.94991927222754
56	32.89252588988442	27.93122898014554	19.51392864399649	19.662316485973555
57	33.24334532798689	27.741542757511272	19.34078867938527	19.67432323511657
58	33.34094942605294	28.253624318661817	19.335966646572547	19.069459608712698
59	33.09324373885514	28.617004683475468	19.199804398504323	19.08994717916507
60	32.86020577044194	28.49000783253055	19.717489307204662	18.932297089822853
61	33.443837030092304	28.713312041980167	19.13667160167408	18.70617932625345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.5
8	3.0
9	5.0
10	7.0
11	8.0
12	13.0
13	17.0
14	19.0
15	21.0
16	30.5
17	55.5
18	71.0
19	109.0
20	191.0
21	235.0
22	358.5
23	482.0
24	675.0
25	1123.5
26	1379.0
27	1818.0
28	2257.0
29	2890.5
30	4318.5
31	5113.0
32	6015.0
33	6917.0
34	8299.5
35	11202.0
36	12722.0
37	14995.5
38	17269.0
39	19698.0
40	25362.0
41	28597.0
42	32139.0
43	40200.0
44	44719.0
45	48924.5
46	53130.0
47	58623.0
48	69123.0
49	74130.0
50	79154.0
51	84178.0
52	87680.5
53	92256.0
54	93329.0
55	92545.5
56	91762.0
57	89545.5
58	83569.5
59	79810.0
60	75043.5
61	64072.5
62	57868.0
63	51410.0
64	44952.0
65	39540.5
66	29147.0
67	24165.0
68	20284.5
69	16404.0
70	13568.0
71	8828.0
72	6924.0
73	5451.0
74	3978.0
75	3113.5
76	1754.5
77	1260.0
78	948.5
79	637.0
80	478.0
81	254.0
82	189.0
83	146.5
84	85.5
85	67.0
86	57.0
87	47.0
88	37.0
89	27.0
90	27.0
91	26.0
92	25.0
93	23.5
94	23.0
95	24.0
96	35.0
97	46.0
98	67.0
99	378.0
100	668.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.431098951592846E-5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	8.431098951592846E-5
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	3.3724395806371385E-4
57	8.431098951592846E-5
58	0.0
59	0.0
60	5.058659370955707E-4
61	8.431098951592846E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
61	1186085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.53940954105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44355134194254	60.80562511556372
2	15.195490786996793	23.261077850463995
3	3.6036096423504227	8.274544627258074
4	1.0183310598444228	3.1176983215121514
5	0.3244958281850905	1.2418359543910418
6	0.11670653444034035	0.5359589541387522
7	0.0723211959220612	0.38747951432240113
8	0.04594279344338512	0.2813147426258497
9	0.03658999980868548	0.25205192824175277
>10	0.14093994618121514	1.6778866711333194
>50	0.0015457673102702127	0.07152501418404664
>100	3.5632768107071304E-4	0.0323640909039103
>500	1.1877589369023769E-4	0.060637215260978004
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.23455317283331295	0.0	0.0	0.0	0.0
2	0.2349747277808926	0.0	0.0	0.0	0.0
3	0.2355649047075041	0.0	0.0	0.0	0.0
4	0.23573352668653597	0.0	0.0	0.0	0.0
5	0.23666094757121117	0.0	0.0	0.0	1.686219790318569E-4
6	0.2379256124139501	0.0	0.0	0.0	2.5293296854778536E-4
7	0.23935889923572087	0.0	0.0	0.0	2.5293296854778536E-4
8	0.2438273816800651	0.0	0.0	0.0	2.5293296854778536E-4
9	0.24526066850183587	0.0	0.0	0.0	2.5293296854778536E-4
10	0.24787430917682965	0.0	0.0	0.0	2.5293296854778536E-4
11	0.2505722608413394	0.0	0.0	0.0	2.5293296854778536E-4
12	0.255715231201811	0.0	0.0	0.0	2.5293296854778536E-4
13	0.26144837848889413	0.0	0.0	0.0	2.5293296854778536E-4
14	0.2713970752517737	0.0	0.0	0.0	2.5293296854778536E-4
15	0.2843809676372267	0.0	8.431098951592845E-5	0.0	2.5293296854778536E-4
16	0.3020019644460557	0.0	8.431098951592845E-5	0.0	2.5293296854778536E-4
17	0.3286442371330891	0.0	8.431098951592845E-5	0.0	2.5293296854778536E-4
18	0.3750995923563657	0.0	8.431098951592845E-5	0.0	2.5293296854778536E-4
19	0.4343702179860634	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
20	0.5261005745793935	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
21	0.6499534181782924	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
22	0.7981721377472947	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
23	1.0008557565435867	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
24	1.2611237811792577	0.0	8.431098951592845E-5	0.0	4.215549475796423E-4
25	1.5512378961035675	0.0	8.431098951592845E-5	0.0	5.058659370955707E-4
26	1.9314804588204049	0.0	8.431098951592845E-5	0.0	5.058659370955707E-4
27	2.3928301934515654	8.431098951592845E-5	8.431098951592845E-5	0.0	5.058659370955707E-4
28	2.90130977122213	8.431098951592845E-5	8.431098951592845E-5	0.0	5.058659370955707E-4
29	3.5156839518247005	8.431098951592845E-5	8.431098951592845E-5	0.0	5.058659370955707E-4
30	4.196916747113402	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
31	4.941635717507599	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
32	5.823865911802274	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
33	6.757272876733118	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
34	7.757116901402513	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
35	8.861759486040208	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
36	10.050628749204314	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
37	11.289325807172336	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
38	12.604324310652272	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
39	14.011474725673118	8.431098951592845E-5	8.431098951592845E-5	0.0	6.744879161274276E-4
40	15.448555541972118	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
41	16.97070614669269	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
42	18.579191204677574	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
43	20.234553172833312	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
44	21.92473557965913	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
45	23.656230371347753	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
46	25.42549648633951	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
47	27.22275384985056	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
48	29.099179232517063	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
49	30.92147695991434	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
50	32.79259075024134	8.431098951592845E-5	8.431098951592845E-5	0.0	7.587989056433561E-4
>>END_MODULE