##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-20B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 691228 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.279288454750095 40.0 40.0 40.0 40.0 40.0 2 39.158254584594374 40.0 40.0 40.0 40.0 40.0 3 39.155526107160014 40.0 40.0 40.0 40.0 40.0 4 39.06298934649638 40.0 40.0 40.0 40.0 40.0 5 39.22269352514655 40.0 40.0 40.0 40.0 40.0 6 39.26712459564717 40.0 40.0 40.0 40.0 40.0 7 39.28695596821888 40.0 40.0 40.0 40.0 40.0 8 39.31132419404307 40.0 40.0 40.0 40.0 40.0 9 39.337463181468344 40.0 40.0 40.0 40.0 40.0 10-11 39.33447140451486 40.0 40.0 40.0 40.0 40.0 12-13 39.326615819961 40.0 40.0 40.0 40.0 40.0 14-15 39.341042318887546 40.0 40.0 40.0 40.0 40.0 16-17 39.47038893100395 40.0 40.0 40.0 40.0 40.0 18-19 39.48543751121193 40.0 40.0 40.0 40.0 40.0 20-21 39.49782416221565 40.0 40.0 40.0 40.0 40.0 22-23 39.50066258889976 40.0 40.0 40.0 40.0 40.0 24-25 39.495084111176055 40.0 40.0 40.0 40.0 40.0 26-27 39.48769436423294 40.0 40.0 40.0 40.0 40.0 28-29 39.482384972830964 40.0 40.0 40.0 40.0 40.0 30-31 39.45116315785839 40.0 40.0 40.0 40.0 40.0 32-33 39.446485927492795 40.0 40.0 40.0 40.0 40.0 34-35 39.43509677114308 40.0 40.0 40.0 40.0 40.0 36-37 39.42128884798785 40.0 40.0 40.0 40.0 40.0 38-39 39.39956030200289 40.0 40.0 40.0 40.0 40.0 40-41 39.38516357367918 40.0 40.0 40.0 40.0 40.0 42-43 39.369054361256325 40.0 40.0 40.0 40.0 40.0 44-45 39.356745363606834 40.0 40.0 40.0 40.0 40.0 46-47 39.33803467777736 40.0 40.0 40.0 40.0 40.0 48-49 39.32264349365478 40.0 40.0 40.0 40.0 40.0 50-51 39.30802158059453 40.0 40.0 40.0 40.0 40.0 52-53 39.28214228468086 40.0 40.0 40.0 40.0 40.0 54-55 39.253467305645344 40.0 40.0 40.0 40.0 40.0 56-57 39.24341957822347 40.0 40.0 40.0 40.0 40.0 58-59 39.22634508162314 40.0 40.0 40.0 40.0 40.0 60-61 39.183417922794916 40.0 40.0 40.0 40.0 40.0 62-63 39.35663112091463 40.0 40.0 40.0 40.0 40.0 64-65 39.346255833346504 40.0 40.0 40.0 40.0 40.0 66-67 39.352469208103656 40.0 40.0 40.0 40.0 40.0 68-69 39.34164287267755 40.0 40.0 40.0 40.0 40.0 70-71 39.34671714028363 40.0 40.0 40.0 40.0 40.0 72-73 39.33734398724461 40.0 40.0 40.0 40.0 40.0 74-75 39.342090586793056 40.0 40.0 40.0 40.0 40.0 76-77 39.33053800720901 40.0 40.0 40.0 40.0 40.0 78-79 39.337794010263124 40.0 40.0 40.0 40.0 40.0 80-81 39.322140087177175 40.0 40.0 40.0 40.0 40.0 82-83 39.311750333009854 40.0 40.0 40.0 40.0 40.0 84-85 39.318813426625766 40.0 40.0 40.0 40.0 40.0 86-87 39.33852613904024 40.0 40.0 40.0 40.0 40.0 88-89 39.35631692925881 40.0 40.0 40.0 40.0 40.0 90-91 39.466528345498 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3650876029681456 1101 2 0.3322714071302215 1101 3 0.056064484762565314 1101 4 0.17168735427333104 1101 5 -0.027484002634757587 1101 6 0.02726530355033674 1101 7 0.12635985196457966 1101 8 0.06682331232950389 1101 9 0.13583921925378206 1101 10-11 0.010110689438121767 1101 12-13 0.07752982250301699 1101 14-15 -0.024879200214641628 1101 16-17 0.006018325501635502 1101 18-19 0.030748003560091774 1101 20-21 0.0527638131733994 1101 22-23 0.03764786060011005 1101 24-25 0.03547305489989583 1101 26-27 0.13018857102125025 1101 28-29 -0.009857311297935212 1101 30-31 0.010303100747783844 1101 32-33 0.1257385575903598 1101 34-35 0.04230267681806765 1101 36-37 0.10012656058616898 1101 38-39 0.10071524683922917 1101 40-41 0.10976615731168948 1101 42-43 0.19125963324570705 1101 44-45 8.946293003546657E-4 1101 46-47 0.05077489991472817 1101 48-49 0.14220649795660734 1101 50-51 0.03929443060492588 1101 52-53 0.045647718703662576 1101 54-55 0.13630927716841512 1101 56-57 0.08177194046110259 1101 58-59 0.3548594137079135 1101 60-61 0.1203153607366616 1101 62-63 -0.03540949114883318 1101 64-65 0.08554018346021763 1101 66-67 0.09999034815697172 1101 68-69 0.18941749687000708 1101 70-71 0.1901306697793288 1101 72-73 0.2501126745459672 1101 74-75 0.2019069878875257 1101 76-77 0.2854356253621333 1101 78-79 0.30616114028025976 1101 80-81 -0.09713678618544463 1101 82-83 0.2528963350588427 1101 84-85 0.3954007822805039 1101 86-87 0.24323946718192957 1101 88-89 -0.40927996155060953 1101 90-91 0.537709897361502 1102 1 0.32924537266224263 1102 2 0.09424855595857906 1102 3 0.1428042988380227 1102 4 0.0187520926875564 1102 5 0.02537140800612292 1102 6 0.19967630917933832 1102 7 0.030889667913079677 1102 8 0.11302250593354302 1102 9 0.19005111014386955 1102 10-11 -0.04135078001642967 1102 12-13 -0.10671883925429171 1102 14-15 -0.030439191933801624 1102 16-17 0.059007806864819656 1102 18-19 0.01739219366449163 1102 20-21 0.032004539126930354 1102 22-23 0.06932404037317497 1102 24-25 0.013684590896900772 1102 26-27 0.012736703396370785 1102 28-29 0.05110937804245452 1102 30-31 0.0772714265396317 1102 32-33 -0.06764268322805833 1102 34-35 0.027668580120362662 1102 36-37 0.09861912687474472 1102 38-39 -0.010305599644269137 1102 40-41 0.008026385253103285 1102 42-43 0.036184691152080006 1102 44-45 0.02516430036529016 1102 46-47 -0.018805951655060937 1102 48-49 -0.0947167547903689 1102 50-51 0.043964460597983646 1102 52-53 0.0852180041806534 1102 54-55 -0.010464939729466494 1102 56-57 -0.014424868730117169 1102 58-59 0.1546947922660138 1102 60-61 -0.031030436437468722 1102 62-63 0.1322258524084461 1102 64-65 -0.0899559548123463 1102 66-67 0.22824532046048063 1102 68-69 0.09918329841492834 1102 70-71 0.05205580961879264 1102 72-73 0.15262893564320024 1102 74-75 0.08043822076351148 1102 76-77 -0.15554516799927143 1102 78-79 0.02654470871576109 1102 80-81 -0.11879958207082097 1102 82-83 0.12986730002856461 1102 84-85 -0.019207471308007484 1102 86-87 -0.9734354630286859 1102 88-89 0.20951560421390525 1102 90-91 -2.1895628299112246 1103 1 0.17000755047209992 1103 2 0.1871957602211225 1103 3 0.2797344778130011 1103 4 0.12412821506539728 1103 5 0.02397325290319685 1103 6 0.05992494645267499 1103 7 0.07360278265376508 1103 8 0.03256705821790007 1103 9 0.03688112400433141 1103 10-11 0.1207147715953596 1103 12-13 -4.629788341574681E-4 1103 14-15 0.043327235652263596 1103 16-17 -0.07228982311172416 1103 18-19 -0.038453498711930933 1103 20-21 -0.08785125976390162 1103 22-23 -0.004168778208672563 1103 24-25 0.012069119551384233 1103 26-27 -0.015346184422298847 1103 28-29 0.10712460327077622 1103 30-31 0.019403122146350427 1103 32-33 0.05461486485234701 1103 34-35 0.04041347802566264 1103 36-37 -0.03925127992399524 1103 38-39 -0.02301000010162113 1103 40-41 -0.004722455334317033 1103 42-43 0.04119295005754253 1103 44-45 0.0057659029118184435 1103 46-47 0.04457041883802759 1103 48-49 -0.012560661084890512 1103 50-51 -0.06172137104692865 1103 52-53 -0.022392281328258434 1103 54-55 0.17419218524981517 1103 56-57 -0.03174555492554276 1103 58-59 0.032752473992360365 1103 60-61 0.0835644932844346 1103 62-63 0.026226766858165718 1103 64-65 -0.12323836558564238 1103 66-67 -0.0754752984513587 1103 68-69 -0.032986718455902064 1103 70-71 0.15862715922013138 1103 72-73 -0.026225613810801462 1103 74-75 -0.05638130699104238 1103 76-77 0.12245717090402053 1103 78-79 0.07194759263386885 1103 80-81 0.2282612266839621 1103 82-83 -0.03974376808289293 1103 84-85 -0.43850934168643363 1103 86-87 0.03118934987548272 1103 88-89 0.00311725540069574 1103 90-91 -0.6051472454956368 1104 1 0.3059027207481648 1104 2 0.1528088430902912 1104 3 0.17357667246754005 1104 4 0.20626016590991014 1104 5 0.06245213080112677 1104 6 0.11047187711776729 1104 7 0.21396882242081006 1104 8 0.12485097490486652 1104 9 0.21852793862070286 1104 10-11 -0.0784264742349805 1104 12-13 0.014410139017542178 1104 14-15 -0.051587498796394016 1104 16-17 0.05200174271589475 1104 18-19 0.03942301569531281 1104 20-21 0.01194303335114455 1104 22-23 -0.08034273786503121 1104 24-25 0.10205930784304229 1104 26-27 -0.009020689789593916 1104 28-29 -0.014618516256867053 1104 30-31 0.03312303953411089 1104 32-33 -0.048816108497355515 1104 34-35 -0.023249269152614715 1104 36-37 -0.041232160187256284 1104 38-39 0.037778279590867214 1104 40-41 -0.06183818709353517 1104 42-43 0.10202885076268586 1104 44-45 0.06800792196682437 1104 46-47 0.014125992279325317 1104 48-49 0.0278291363997738 1104 50-51 0.09531616968052248 1104 52-53 -0.10193105936010483 1104 54-55 0.08427382880987011 1104 56-57 0.033812559252758945 1104 58-59 -0.26181619736601647 1104 60-61 -0.08296448734271422 1104 62-63 -0.024109138510823414 1104 64-65 0.07898993400061016 1104 66-67 -0.004387017372884827 1104 68-69 0.07627209319882411 1104 70-71 -0.00812314018267557 1104 72-73 0.19272422555120983 1104 74-75 -0.01582329256400783 1104 76-77 -0.27228867752788943 1104 78-79 0.020250313663865427 1104 80-81 0.256128568512068 1104 82-83 0.10999054033786848 1104 84-85 0.14134615635088466 1104 86-87 -0.11916127670840382 1104 88-89 -0.43453179596326663 1104 90-91 0.1134674731190799 1201 1 0.36279463369242393 1201 2 0.1386532324243248 1201 3 0.16363153493243487 1201 4 0.22745568765068924 1201 5 0.12726815417767767 1201 6 0.14146095936093417 1201 7 0.047453031041328586 1201 8 0.08987878507989677 1201 9 -0.005351338693692753 1201 10-11 0.08708301406622354 1201 12-13 -0.012869516397337577 1201 14-15 -0.1176708745853361 1201 16-17 -0.02649831833596039 1201 18-19 0.015638052979049633 1201 20-21 -0.019450254312587845 1201 22-23 0.047285123677774266 1201 24-25 -0.023151742122642816 1201 26-27 0.050300666151173345 1201 28-29 -0.044554980217320406 1201 30-31 0.07949719059745775 1201 32-33 0.026863168227151846 1201 34-35 0.048556656010937616 1201 36-37 -0.05151941132368876 1201 38-39 0.027724615826521415 1201 40-41 0.011326451232548607 1201 42-43 -0.034221927618141024 1201 44-45 0.01002489311936472 1201 46-47 -0.04821322902777325 1201 48-49 -0.0020492389687518653 1201 50-51 -7.008319298265064E-4 1201 52-53 -0.0053046379489174456 1201 54-55 0.01705056570630603 1201 56-57 0.086775630011374 1201 58-59 0.045992722562118615 1201 60-61 0.05923119581054692 1201 62-63 -0.21513992933557802 1201 64-65 -0.08453465150797257 1201 66-67 -0.21285167672359506 1201 68-69 -0.17602014230641316 1201 70-71 -0.4373817696562625 1201 72-73 -0.287116818374173 1201 74-75 -0.3879988911796417 1201 76-77 -0.40014103902447573 1201 78-79 0.03910327633645494 1201 80-81 -0.13927373416871802 1201 82-83 -0.49628569666077027 1201 84-85 -0.7555193330519216 1201 86-87 0.062895637343793 1201 88-89 -0.24443176420714963 1201 90-91 0.26184782839598597 1202 1 0.16173019862533522 1202 2 0.13653147432845714 1202 3 0.08971375009529936 1202 4 0.044913371022744286 1202 5 0.1793662054791767 1202 6 0.023787544160136065 1202 7 0.025730495362601857 1202 8 0.10705198691954365 1202 9 0.07292612562758194 1202 10-11 0.06069616659573285 1202 12-13 -0.03778535727733612 1202 14-15 0.05006062226166108 1202 16-17 0.04161959925922076 1202 18-19 0.0031967272951121117 1202 20-21 -0.011207401911093484 1202 22-23 0.003090917618315814 1202 24-25 -0.03666224423254505 1202 26-27 0.007218308747539481 1202 28-29 -0.054690820149048136 1202 30-31 0.049540099895260425 1202 32-33 0.06002407973320345 1202 34-35 -0.005142618564747181 1202 36-37 0.03592265447890952 1202 38-39 -0.09463298720007174 1202 40-41 0.13864323702642167 1202 42-43 -0.03889801862215592 1202 44-45 0.018919478784980015 1202 46-47 -0.02451336063436571 1202 48-49 0.08203877342953092 1202 50-51 -0.09267518759851612 1202 52-53 0.026202532794485478 1202 54-55 0.04284664311364139 1202 56-57 -0.007470218115216198 1202 58-59 0.0600477226328735 1202 60-61 -0.10348080879192878 1202 62-63 -0.18094553074593733 1202 64-65 0.11567357177306548 1202 66-67 -0.04895342685905035 1202 68-69 -0.013008529585334827 1202 70-71 -0.29568371309630237 1202 72-73 -0.1071713772277505 1202 74-75 -0.14131572234992262 1202 76-77 -0.013272441304096105 1202 78-79 -0.03784776713414573 1202 80-81 0.11775435421719749 1202 82-83 0.0054180128420284746 1202 84-85 0.254249872286465 1202 86-87 0.061800414155420924 1202 88-89 0.02467763270729506 1202 90-91 0.537709897361502 1203 1 0.25501739746964347 1203 2 0.056040771127001676 1203 3 0.07000174114800473 1203 4 0.12776904929293664 1203 5 0.17903068192262594 1203 6 -0.07367039889405902 1203 7 -0.03534644205293347 1203 8 0.04443024312906374 1203 9 -0.06242733911913945 1203 10-11 0.033701665454024976 1203 12-13 0.09868418929985978 1203 14-15 -0.006914306672584303 1203 16-17 0.062353324036699576 1203 18-19 0.10812239950723068 1203 20-21 0.11393123455845 1203 22-23 0.08925057091064303 1203 24-25 0.07156973302640779 1203 26-27 0.07417622850148575 1203 28-29 0.060411234932743696 1203 30-31 0.05235593474311173 1203 32-33 0.10939644353928912 1203 34-35 0.0631776477221564 1203 36-37 0.020594944116886893 1203 38-39 0.07966347901485449 1203 40-41 0.07695665913907135 1203 42-43 -0.21416187779809093 1203 44-45 -0.13943470176204187 1203 46-47 -0.03120775881601645 1203 48-49 0.19499576538572683 1203 50-51 0.03242858504331991 1203 52-53 0.10793689129130968 1203 54-55 -0.07804117774566066 1203 56-57 0.015831631273201197 1203 58-59 0.08029924148540601 1203 60-61 0.058691426092913446 1203 62-63 -0.13563961932822366 1203 64-65 -0.1513943462282228 1203 66-67 -0.1475132410286264 1203 68-69 -0.07353297502729816 1203 70-71 0.025945614344514922 1203 72-73 -0.03139682705920421 1203 74-75 0.2320246119750209 1203 76-77 -0.0671533547087293 1203 78-79 -0.0526365758833478 1203 80-81 0.4075202484930216 1203 82-83 0.1980297251782659 1203 84-85 0.05958347458820157 1203 86-87 0.2556826469369824 1203 88-89 0.07365766668715423 1203 90-91 0.24141360106520438 1204 1 0.25697645516511614 1204 2 0.21135557680003814 1204 3 0.019715554172513805 1204 4 -0.05647576843242064 1204 5 -0.013561811839963411 1204 6 0.03489163941541307 1204 7 -0.154235227170318 1204 8 -0.11116919294695293 1204 9 -0.1800686425484912 1204 10-11 -0.12037455323107338 1204 12-13 0.06342337445638435 1204 14-15 -0.08941709738473946 1204 16-17 -0.11452779384919864 1204 18-19 -0.03794525358167533 1204 20-21 -0.1264969563040239 1204 22-23 -0.051247888082563975 1204 24-25 -0.09514140714072283 1204 26-27 -0.0914286333415788 1204 28-29 -0.14907562981889555 1204 30-31 -0.08590550717671164 1204 32-33 0.04689429407489598 1204 34-35 -0.09440641099136826 1204 36-37 -0.06873912080166633 1204 38-39 -0.18258252845849654 1204 40-41 -0.02985223868018494 1204 42-43 -0.04432776461297294 1204 44-45 -0.02465023989540782 1204 46-47 -0.03896282250061489 1204 48-49 -0.09305230571057166 1204 50-51 -0.06565617065260199 1204 52-53 -0.03977105442562845 1204 54-55 -0.06790893009257815 1204 56-57 -0.13716374089661798 1204 58-59 -0.034989038110680326 1204 60-61 -0.02560141358451773 1204 62-63 0.01853940585251479 1204 64-65 -0.11518481053641239 1204 66-67 -0.08631120681688031 1204 68-69 -0.2505433048764161 1204 70-71 -0.14857486425392352 1204 72-73 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0.10999991429972766 2401 54-55 0.10398498652831023 2401 56-57 0.09368897555796707 2401 58-59 0.15714235687934774 2401 60-61 0.13540105129444413 2401 62-63 0.11930674806652064 2401 64-65 0.022949125636536394 2401 66-67 0.15971513310329755 2401 68-69 0.19760864714407234 2401 70-71 0.1227634683988228 2401 72-73 0.2763828886698363 2401 74-75 0.10915225069027201 2401 76-77 0.27009331629262334 2401 78-79 0.39215575030332417 2401 80-81 0.2554275863387474 2401 82-83 0.3735528424273369 2401 84-85 0.08205460318283997 2401 86-87 0.09337194340136534 2401 88-89 0.2827421488363271 2401 90-91 0.537709897361502 2402 1 0.27879212220899774 2402 2 0.3485868102968297 2402 3 0.3015714576051849 2402 4 0.21750889144435348 2402 5 0.14339782603254037 2402 6 0.21403787354090298 2402 7 0.11466330168671846 2402 8 0.05026674317777946 2402 9 0.14367711376987558 2402 10-11 0.13223766857201724 2402 12-13 0.08415140161594792 2402 14-15 0.09650331000869272 2402 16-17 0.07208731994433748 2402 18-19 -0.025459399187106158 2402 20-21 0.050024482146881155 2402 22-23 0.0353043563534996 2402 24-25 0.02150639871870652 2402 26-27 0.0944383350980047 2402 28-29 0.06681774375081773 2402 30-31 0.01841361768182992 2402 32-33 0.06721802319329839 2402 34-35 0.056266600541263756 2402 36-37 0.1498501235288785 2402 38-39 0.018006615746962495 2402 40-41 0.06543351810206133 2402 42-43 0.06294986692375915 2402 44-45 0.05709580109132162 2402 46-47 0.09772770514267393 2402 48-49 0.16974885758548908 2402 50-51 0.0568018735663145 2402 52-53 0.15742566493882038 2402 54-55 0.12136142578558662 2402 56-57 0.11214900327800592 2402 58-59 0.043405276683564864 2402 60-61 0.11734613899703561 2402 62-63 0.17985249687263405 2402 64-65 0.09955231192817138 2402 66-67 0.10746181962760915 2402 68-69 0.12981154279434293 2402 70-71 0.04333912243958338 2402 72-73 -0.0391420203849151 2402 74-75 0.002472643055853041 2402 76-77 -0.0896805660933282 2402 78-79 -0.0010782484332096942 2402 80-81 0.21393712814933963 2402 82-83 0.28667294125866505 2402 84-85 0.47105066412287044 2402 86-87 0.2805773306067536 2402 88-89 -0.07712407189946191 2402 90-91 0.537709897361502 2403 1 -0.38517894502374617 2403 2 -0.17794462713156634 2403 3 -0.11852423617472141 2403 4 -0.05216336859113824 2403 5 0.09609679496575296 2403 6 -0.04339748823100109 2403 7 0.1083859245049652 2403 8 0.052151416448666055 2403 9 0.061511873524580096 2403 10-11 0.13472604379946063 2403 12-13 0.16292692557308186 2403 14-15 0.10773216640063765 2403 16-17 0.08711304045559842 2403 18-19 0.08440986424034946 2403 20-21 0.05542972059510731 2403 22-23 0.02812395336137996 2403 24-25 -0.014160874457829209 2403 26-27 0.015051808602507322 2403 28-29 0.0697548239673722 2403 30-31 0.07674301993265686 2403 32-33 0.06279185665775344 2403 34-35 0.09753260643497441 2403 36-37 0.02679372876476549 2403 38-39 0.04498979871100772 2403 40-41 0.024304573543226127 2403 42-43 0.14930666283216 2403 44-45 0.016069698972884794 2403 46-47 0.1384647948317408 2403 48-49 -0.001658636847665207 2403 50-51 0.06123466170348024 2403 52-53 0.06969400227438172 2403 54-55 0.061766401249577996 2403 56-57 -0.050776366419412966 2403 58-59 0.01493340282438993 2403 60-61 0.09320989344944763 2403 62-63 0.18162543804593412 2403 64-65 0.1771229820011868 2403 66-67 -0.046786619616085545 2403 68-69 0.18288220428526358 2403 70-71 0.11325458272764166 2403 72-73 0.14659933888628984 2403 74-75 0.267473988525353 2403 76-77 0.1015744742599054 2403 78-79 0.10662743792824614 2403 80-81 0.3691425162863524 2403 82-83 0.2985082791474838 2403 84-85 -0.010022456197525287 2403 86-87 0.0062471653497837565 2403 88-89 0.21152061674523281 2403 90-91 -0.567744648093047 2404 1 -0.3856962659783818 2404 2 -0.2194946028613316 2404 3 -0.3512381812770897 2404 4 -0.2236719170146273 2404 5 -0.1727963130369261 2404 6 -0.08407712141048052 2404 7 -0.14488708802091566 2404 8 -0.13100573450054043 2404 9 -0.10334477112171925 2404 10-11 0.047032093540536835 2404 12-13 0.02741575613077174 2404 14-15 0.11569513465727965 2404 16-17 0.01300265071365203 2404 18-19 0.034193097324248356 2404 20-21 0.023146284218512392 2404 22-23 -0.010284629725944683 2404 24-25 0.0060973475178158765 2404 26-27 0.04903541030533631 2404 28-29 0.04654670691296303 2404 30-31 -0.007506194441653236 2404 32-33 0.055257461749761205 2404 34-35 0.019318304837092626 2404 36-37 0.040033418917701624 2404 38-39 0.05419027302838231 2404 40-41 0.03737192813583334 2404 42-43 0.015492868493453216 2404 44-45 0.0462956665001002 2404 46-47 0.06526893546661938 2404 48-49 0.06572666331193489 2404 50-51 0.09257215042715927 2404 52-53 0.13381557061615723 2404 54-55 0.08684018868715526 2404 56-57 0.01779552818114638 2404 58-59 0.09082342391121756 2404 60-61 0.0033005582190241967 2404 62-63 0.07133208969111848 2404 64-65 0.009774433451489983 2404 66-67 0.043697505494805 2404 68-69 -0.028146296262299586 2404 70-71 0.03297222378703424 2404 72-73 0.15528003010354752 2404 74-75 0.1895514760891288 2404 76-77 0.04531355170875173 2404 78-79 0.02845171490980647 2404 80-81 -0.055438760090495975 2404 82-83 -0.16449069819733353 2404 84-85 -0.02556546478173516 2404 86-87 -0.08251885150171034 2404 88-89 0.3228759281005438 2404 90-91 -0.16229010263850085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 20.0 31 402.0 32 2779.0 33 5072.0 34 6830.0 35 8471.0 36 12469.0 37 19493.0 38 53882.0 39 317618.0 40 264191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.88973634869563 11.435354505682739 32.707576213513576 29.967332932108054 2 27.12423265174434 17.66488854551426 29.14295717896522 26.067921623776186 3 26.93468398088029 19.8844368829786 22.163292941217318 31.017586194923787 4 26.3348706938955 20.588141684075296 20.98178893216131 32.095198689867885 5 26.993408831818154 21.094197573014984 20.8229412002986 31.08945239486826 6 28.06845208816772 22.87552008888529 23.70722829515008 25.348799527796906 7 25.91228943271974 22.727233271800333 23.557494777410636 27.802982518069292 8 25.054685284739627 24.2605912954915 23.96416233138704 26.72056108838184 9 25.44341375060038 24.387322272824598 24.259144594837014 25.910119381738006 10-11 24.876379429074053 23.094188892811054 24.324535464419846 27.704896213695047 12-13 25.4957119792601 22.813022620611434 23.931466896595623 27.759798503532846 14-15 25.94310415666032 22.501620304733024 23.88358110493209 27.67169443367456 16-17 25.853191710983932 22.138136765293073 23.729001140000115 28.279670383722884 18-19 26.19287702465757 22.367004808832974 23.629685140069554 27.810433026439902 20-21 26.180146058898078 22.41988171775449 23.60306584802699 27.796906375320447 22-23 25.789464547153763 21.929377860850543 23.972553195183067 28.30860439681263 24-25 25.888925217149765 22.2783220587129 24.033676297835157 27.79907642630218 26-27 26.11048742238451 22.302409624610114 23.958954209030882 27.62814874397449 28-29 25.918365575468588 22.004389289785713 24.13255828756937 27.94468684717633 30-31 26.07607792247248 22.2797770047546 24.090546428046995 27.553598644725923 32-33 26.210896376500482 22.316707738091736 24.151762346423258 27.320633538984524 34-35 25.887428272164513 21.9448180759797 24.470014656403414 27.69773899545237 36-37 26.137036614453447 22.23614160827898 24.411446702851897 27.215375074415675 38-39 26.17871485943775 22.198072289156627 24.41839357429719 27.204819277108435 40-41 25.92490200778592 22.00629923101273 24.53409327238453 27.53470548881682 42-43 26.104452383696362 22.299548440495773 24.544075071331033 27.051924104476832 44-45 26.330470823953377 22.302998069754754 24.386195766298194 26.980335339993673 46-47 25.96665075472009 21.993150439547353 24.722420449142934 27.317778356589628 48-49 26.20701707495091 22.228173155010946 24.61280672718114 26.952003042856997 50-51 26.381685282126742 22.337894175879363 24.370097130814088 26.910323411179803 52-53 26.219740300073642 22.016144780709084 24.565735514517833 27.198379404699445 54-55 26.30218680301763 22.34837281392689 24.500774971406667 26.848665411648813 56-57 26.50112698978756 22.25546662334032 24.453544200443087 26.78986218642903 58-59 26.253075053841602 22.031653154952675 24.531946017223305 27.183325773982414 60-61 26.399279578830704 22.27450553510404 24.5710129438309 26.755201942234365 62-63 26.609083005407808 22.3054364249769 24.3967476592588 26.688732910356492 64-65 26.34299929243801 21.975577960558994 24.541655938350875 27.139766808652126 66-67 26.563839877717854 22.253257786502108 24.448240488218744 26.7346618475613 68-69 26.81535243049309 22.22345013338542 24.265352173488427 26.695845262633068 70-71 26.54177981360813 22.0154133993349 24.28402179459358 27.158784992463385 72-73 26.709710621383614 22.338390490152594 24.321414423900567 26.630484464563224 74-75 26.829799535867316 22.197581124214896 24.249744568617977 26.72287477129981 76-77 26.576877167589313 22.034925796779984 24.401063939772737 26.987133095857967 78-79 26.798563195525897 22.247166744342014 24.089777545383242 26.864492514748843 80-81 27.105535637559786 22.065069626895635 23.86614167682132 26.963253058723257 82-83 27.041407886103595 21.541628093008207 24.043897572331225 27.37306644855697 84-85 28.017526753578174 21.30535793819893 23.319249334922297 27.357865973300594 86-87 28.4605904917293 20.641174734868866 23.031260305833754 27.866974467568085 88-89 27.193691872219976 19.993067991450523 23.84322107330599 28.97001906302351 90-91 23.06871532223645 19.732536633945085 28.702518139137855 28.496229904680607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 0.5 7 0.0 8 0.5 9 2.5 10 4.0 11 4.0 12 8.0 13 12.5 14 15.0 15 25.5 16 38.5 17 53.0 18 91.5 19 154.5 20 222.0 21 313.0 22 439.0 23 607.0 24 848.5 25 1140.5 26 1475.5 27 1899.0 28 2460.0 29 3105.0 30 3837.5 31 4681.5 32 5593.5 33 6582.0 34 7874.0 35 9337.5 36 10783.5 37 12284.0 38 14147.0 39 16277.0 40 18308.0 41 20542.0 42 22901.5 43 25286.0 44 27797.5 45 30672.0 46 33700.0 47 36531.0 48 39082.0 49 41566.0 50 44161.5 51 46584.0 52 48286.5 53 49381.0 54 50244.5 55 50281.0 56 49749.0 57 49374.0 58 48438.0 59 46886.0 60 45276.0 61 43193.5 62 40883.5 63 38083.5 64 34600.0 65 31118.0 66 27441.0 67 24136.0 68 21168.0 69 17945.0 70 14988.5 71 12448.0 72 10106.5 73 7944.0 74 6099.0 75 4657.0 76 3546.0 77 2665.0 78 2035.0 79 1503.5 80 1066.0 81 756.5 82 548.5 83 389.5 84 260.5 85 178.5 86 133.0 87 99.0 88 71.0 89 50.0 90 34.0 91 26.5 92 20.0 93 12.5 94 8.5 95 6.0 96 3.0 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0017360407853848512 2 0.007088833206988143 3 5.786802617949504E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 3.960160782527771E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7259.0 32-33 15912.0 34-35 18821.0 36-37 20972.0 38-39 23446.0 40-41 25397.0 42-43 27105.0 44-45 28539.0 46-47 29956.0 48-49 30095.0 50-51 31016.0 52-53 31159.0 54-55 30697.0 56-57 30357.0 58-59 30104.0 60-61 28980.0 62-63 27894.0 64-65 27390.0 66-67 25366.0 68-69 24443.0 70-71 23219.0 72-73 21621.0 74-75 20303.0 76-77 18753.0 78-79 17336.0 80-81 15779.0 82-83 14550.0 84-85 12866.0 86-87 11817.0 88-89 10697.0 90-91 9379.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.72303026113775 #Duplication Level Percentage of deduplicated Percentage of total 1 99.75280048684384 99.47651541582765 2 0.22583538935680053 0.4504197873372809 3 0.015887919310845638 0.04753174374665923 4 0.004056680792286346 0.0161817800563579 5 8.025156461879804E-4 0.004001464603492024 6 2.3840034669701026E-4 0.0014264402992759011 7 2.181063372521788E-4 0.0015225157408961458 8 0.0 0.0 9 0.0 0.0 >10 1.6050136610799658E-4 0.002400852388401253 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.233503272436881E-4 0.0 0.0 0.0 0.0 2 7.233503272436881E-4 0.0 0.0 0.0 0.0 3 7.233503272436881E-4 0.0 0.0 0.0 0.0 4 8.680203926924256E-4 0.0 0.0 0.0 0.0 5 8.680203926924256E-4 0.0 0.0 0.0 0.0 6 8.680203926924256E-4 0.0 0.0 0.0 0.0 7 8.680203926924256E-4 0.0 0.0 1.446700654487376E-4 0.0 8 0.00303807137442349 0.0 0.0 1.446700654487376E-4 1.446700654487376E-4 9 0.00303807137442349 0.0 0.0 1.446700654487376E-4 2.893401308974752E-4 10-11 0.00303807137442349 0.0 7.23350327243688E-5 1.446700654487376E-4 2.893401308974752E-4 12-13 0.00303807137442349 0.0 1.446700654487376E-4 1.446700654487376E-4 2.893401308974752E-4 14-15 0.00303807137442349 0.0 1.446700654487376E-4 1.446700654487376E-4 2.893401308974752E-4 16-17 0.00303807137442349 0.0 1.446700654487376E-4 1.446700654487376E-4 3.6167516362184404E-4 18-19 0.00303807137442349 7.23350327243688E-5 1.446700654487376E-4 1.446700654487376E-4 4.340101963462128E-4 20-21 0.00303807137442349 1.446700654487376E-4 1.446700654487376E-4 1.446700654487376E-4 4.340101963462128E-4 22-23 0.0031827414398722274 2.170050981731064E-4 1.446700654487376E-4 1.446700654487376E-4 4.340101963462128E-4 24-25 0.003327411505320965 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 4.340101963462128E-4 26-27 0.0034720815707697025 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 5.063452290705816E-4 28-29 0.0039784267998402845 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 5.786802617949504E-4 30-31 0.0042677669307377595 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 5.786802617949504E-4 32-33 0.0047017771270839726 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 5.786802617949504E-4 34-35 0.005135787323430185 2.893401308974752E-4 1.446700654487376E-4 1.446700654487376E-4 6.510152945193193E-4 36-37 0.005497462487052029 2.893401308974752E-4 2.170050981731064E-4 1.446700654487376E-4 7.233503272436881E-4 38-39 0.005859137650673873 2.893401308974752E-4 2.893401308974752E-4 1.446700654487376E-4 7.233503272436881E-4 40-41 0.006003807716122611 4.340101963462128E-4 2.893401308974752E-4 1.446700654487376E-4 7.233503272436881E-4 42-43 0.006220812814295717 4.340101963462128E-4 2.893401308974752E-4 1.446700654487376E-4 7.233503272436881E-4 44-45 0.006365482879744455 4.340101963462128E-4 2.893401308974752E-4 1.446700654487376E-4 8.680203926924256E-4 46-47 0.006510152945193193 4.340101963462128E-4 2.893401308974752E-4 1.446700654487376E-4 8.680203926924256E-4 48-49 0.00665482301064193 4.340101963462128E-4 2.893401308974752E-4 1.446700654487376E-4 8.680203926924256E-4 50-51 0.00665482301064193 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