##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-20A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 840876 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25814745574853 40.0 40.0 40.0 40.0 40.0 2 39.17270085006589 40.0 40.0 40.0 40.0 40.0 3 39.15771171968281 40.0 40.0 40.0 40.0 40.0 4 39.06545079179332 40.0 40.0 40.0 40.0 40.0 5 39.20740275617332 40.0 40.0 40.0 40.0 40.0 6 39.258102264781016 40.0 40.0 40.0 40.0 40.0 7 39.3014998644271 40.0 40.0 40.0 40.0 40.0 8 39.32424757039088 40.0 40.0 40.0 40.0 40.0 9 39.345454026515206 40.0 40.0 40.0 40.0 40.0 10-11 39.34240006849999 40.0 40.0 40.0 40.0 40.0 12-13 39.34527564111712 40.0 40.0 40.0 40.0 40.0 14-15 39.3530365951698 40.0 40.0 40.0 40.0 40.0 16-17 39.48109828321893 40.0 40.0 40.0 40.0 40.0 18-19 39.490350539199596 40.0 40.0 40.0 40.0 40.0 20-21 39.50589385355272 40.0 40.0 40.0 40.0 40.0 22-23 39.50565124941133 40.0 40.0 40.0 40.0 40.0 24-25 39.50514701335274 40.0 40.0 40.0 40.0 40.0 26-27 39.50126772556239 40.0 40.0 40.0 40.0 40.0 28-29 39.49549993102431 40.0 40.0 40.0 40.0 40.0 30-31 39.47873933947608 40.0 40.0 40.0 40.0 40.0 32-33 39.48072143411718 40.0 40.0 40.0 40.0 40.0 34-35 39.477460518776766 40.0 40.0 40.0 40.0 40.0 36-37 39.47009709025876 40.0 40.0 40.0 40.0 40.0 38-39 39.4593686870957 40.0 40.0 40.0 40.0 40.0 40-41 39.437258431168694 40.0 40.0 40.0 40.0 40.0 42-43 39.43479852403241 40.0 40.0 40.0 40.0 40.0 44-45 39.422119690018704 40.0 40.0 40.0 40.0 40.0 46-47 39.412196964301 40.0 40.0 40.0 40.0 40.0 48-49 39.415623946337945 40.0 40.0 40.0 40.0 40.0 50-51 39.408233352755346 40.0 40.0 40.0 40.0 40.0 52-53 39.391779754242265 40.0 40.0 40.0 40.0 40.0 54-55 39.3715402019815 40.0 40.0 40.0 40.0 40.0 56-57 39.365840893368585 40.0 40.0 40.0 40.0 40.0 58-59 39.35009984419263 40.0 40.0 40.0 40.0 40.0 60-61 39.31408129191399 40.0 40.0 40.0 40.0 40.0 62-63 39.35896523402674 40.0 40.0 40.0 40.0 40.0 64-65 39.35457360549124 40.0 40.0 40.0 40.0 40.0 66-67 39.347691566750086 40.0 40.0 40.0 40.0 40.0 68-69 39.343810856655224 40.0 40.0 40.0 40.0 40.0 70-71 39.347433282095764 40.0 40.0 40.0 40.0 40.0 72-73 39.331527830922035 40.0 40.0 40.0 40.0 40.0 74-75 39.340793935073904 40.0 40.0 40.0 40.0 40.0 76-77 39.32054953438231 40.0 40.0 40.0 40.0 40.0 78-79 39.31281030197725 40.0 40.0 40.0 40.0 40.0 80-81 39.324910645224364 40.0 40.0 40.0 40.0 40.0 82-83 39.30045649843939 40.0 40.0 40.0 40.0 40.0 84-85 39.321123688480675 40.0 40.0 40.0 40.0 40.0 86-87 39.32060792474982 40.0 40.0 40.0 40.0 40.0 88-89 39.32905345614178 40.0 40.0 40.0 40.0 40.0 90-91 39.41757853706035 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0768501922200997 1101 2 0.18333020253012933 1101 3 0.04255790883111388 1101 4 -0.06898128064250386 1101 5 0.13600053509108534 1101 6 -0.03505006065724103 1101 7 0.04057188505042575 1101 8 -0.06097654180549483 1101 9 -0.055774822582833394 1101 10-11 -0.014944235367529757 1101 12-13 -0.10756672960621216 1101 14-15 -0.11097005842069052 1101 16-17 -0.03632355081343519 1101 18-19 -0.029676210417903803 1101 20-21 0.025686996727039002 1101 22-23 0.014367827210783446 1101 24-25 -0.03747950416134671 1101 26-27 0.044061125874407026 1101 28-29 -0.07056678024342489 1101 30-31 -0.009852601839810404 1101 32-33 0.015203561051237102 1101 34-35 -0.04963638146134741 1101 36-37 0.06864169141916676 1101 38-39 -0.032010096058741055 1101 40-41 -0.07136774665193713 1101 42-43 0.06690360498601677 1101 44-45 0.04234358268545435 1101 46-47 -0.06404632528341381 1101 48-49 -0.0403135356077442 1101 50-51 -0.07927939344438784 1101 52-53 -0.014584853710296386 1101 54-55 0.03866132240636233 1101 56-57 -0.017303409190120078 1101 58-59 -0.06336961018946852 1101 60-61 0.056566051530523964 1101 62-63 0.00974824477589209 1101 64-65 0.023564654941715446 1101 66-67 0.07403872794807143 1101 68-69 -0.01179441757590638 1101 70-71 -0.11850279466084856 1101 72-73 -0.18554397647951504 1101 74-75 0.08801824066989639 1101 76-77 -0.1898515542129573 1101 78-79 -0.08884161222903231 1101 80-81 0.10778696049911218 1101 82-83 -0.15309476348748774 1101 84-85 -0.010320008275883197 1101 86-87 0.36754947428496365 1101 88-89 -0.2269967253218823 1101 90-91 0.5958829476070946 1102 1 0.40109512759141097 1102 2 0.1675377899305346 1102 3 0.24512794781796998 1102 4 0.10267333435422898 1102 5 0.015258183839272021 1102 6 0.10616883290860102 1102 7 0.22319460738133046 1102 8 0.16046305690631613 1102 9 -0.019045470724783797 1102 10-11 0.0033054462472108526 1102 12-13 0.008150286173822963 1102 14-15 1.282988603463764E-4 1102 16-17 0.11015276528915052 1102 18-19 -0.028522070402260624 1102 20-21 0.03737696618009778 1102 22-23 0.08004426972318157 1102 24-25 0.07423747216807897 1102 26-27 0.1196770222015715 1102 28-29 -0.00800609608601377 1102 30-31 -0.03888446932439393 1102 32-33 0.008461644180542294 1102 34-35 -0.04564238927656561 1102 36-37 0.009580265087315354 1102 38-39 -0.007678817022892304 1102 40-41 0.01671528291461044 1102 42-43 0.03890945014669711 1102 44-45 0.03014085156075197 1102 46-47 -0.04339910413326464 1102 48-49 -0.03238101864964449 1102 50-51 0.06172891448353823 1102 52-53 -0.07081962731117386 1102 54-55 -0.08298718810171835 1102 56-57 0.08054852525678768 1102 58-59 0.051503701225037446 1102 60-61 -0.0871874479360315 1102 62-63 0.004964559469350149 1102 64-65 0.08189151531739469 1102 66-67 0.05468991346204177 1102 68-69 0.08672402965760995 1102 70-71 -0.10213251497353326 1102 72-73 -0.1382919872374444 1102 74-75 -0.15110227328909787 1102 76-77 -0.2367792424713926 1102 78-79 -0.12936685748834975 1102 80-81 -0.10503745152009003 1102 82-83 -0.010748605675814815 1102 84-85 0.44364536100070495 1102 86-87 0.4041730688032956 1102 88-89 0.2649640589918434 1102 90-91 -0.3052159534918104 1103 1 0.2871728552231474 1103 2 0.1938749010400258 1103 3 0.02986484414470425 1103 4 -0.00810295337974054 1103 5 0.08658936865129618 1103 6 0.05137009288418426 1103 7 0.029474713648845352 1103 8 0.02699472123333635 1103 9 0.0684168980667863 1103 10-11 0.04697960498873499 1103 12-13 0.03546599081006718 1103 14-15 0.01194176669652336 1103 16-17 0.03746004095095401 1103 18-19 0.017608353928963538 1103 20-21 0.0067275501207220145 1103 22-23 -0.011760373705186566 1103 24-25 -0.020248209501815495 1103 26-27 -0.02232393308219116 1103 28-29 -0.014795478908176563 1103 30-31 0.03920154992072611 1103 32-33 -0.03278503672164845 1103 34-35 -0.07858860345432106 1103 36-37 0.030174854899065906 1103 38-39 -4.160069943424105E-4 1103 40-41 0.07205515487214598 1103 42-43 0.023825917948201436 1103 44-45 0.05885185576374141 1103 46-47 0.04573475167292429 1103 48-49 -0.007906791563343063 1103 50-51 -0.02887603059356536 1103 52-53 -0.03953092913177869 1103 54-55 0.016616954591668787 1103 56-57 0.027269323775875876 1103 58-59 0.050296351351022395 1103 60-61 0.01998934672218411 1103 62-63 0.04658220360355614 1103 64-65 -0.02823900467115692 1103 66-67 -0.08852629481727803 1103 68-69 -0.04158805007440236 1103 70-71 -0.046410328911079546 1103 72-73 -0.0888450727354737 1103 74-75 -0.08264166482220503 1103 76-77 -0.09910357068824283 1103 78-79 -0.1522615352743273 1103 80-81 0.02937623431347447 1103 82-83 -0.10171587172405339 1103 84-85 0.017630736669353553 1103 86-87 -0.7299628228484565 1103 88-89 0.17496405899183998 1103 90-91 -0.64048068875654 1104 1 0.3433271484775702 1104 2 0.24917125369202608 1104 3 0.14293053122247557 1104 4 0.1901021340399609 1104 5 0.018356680274010273 1104 6 0.02646420524679627 1104 7 0.06897599565780155 1104 8 0.20637553541656928 1104 9 0.18050299624776045 1104 10-11 0.1072507326263974 1104 12-13 0.10918245624684886 1104 14-15 0.06409327084367078 1104 16-17 -0.06034398724824541 1104 18-19 0.046909986677164284 1104 20-21 0.10587050987573576 1104 22-23 -0.02074514065766664 1104 24-25 -0.001216653974267956 1104 26-27 0.06251814922448062 1104 28-29 0.019849475138578043 1104 30-31 0.022533732797327843 1104 32-33 0.0013359633796881099 1104 34-35 0.016789389816366906 1104 36-37 0.0029266322635166375 1104 38-39 0.03965500178168213 1104 40-41 0.09073167451273889 1104 42-43 0.09979640106342202 1104 44-45 0.0209469234854609 1104 46-47 0.0076498451855187 1104 48-49 -0.031032497027290162 1104 50-51 0.028934600911725283 1104 52-53 0.06391153683239992 1104 54-55 -0.025694804792408377 1104 56-57 -0.07936547073512656 1104 58-59 -0.04206447326485829 1104 60-61 0.06808845961440824 1104 62-63 0.05632225056972828 1104 64-65 0.08360408383703799 1104 66-67 -0.051576739415082784 1104 68-69 0.1757227826227421 1104 70-71 0.09470877594023364 1104 72-73 -0.009659231281169411 1104 74-75 0.2423740294538561 1104 76-77 0.12467834970886571 1104 78-79 0.1959255506261428 1104 80-81 0.18885737913944922 1104 82-83 0.10077300447140658 1104 84-85 -0.01418679005648471 1104 86-87 -0.08213034548047204 1104 88-89 -0.5552246202534405 1104 90-91 -0.8755885238643799 1201 1 0.22342380182902843 1201 2 0.16685143328109575 1201 3 0.2507959514038447 1201 4 0.1719682576001773 1201 5 0.02293389184931982 1201 6 0.16077806968364428 1201 7 0.15258359424894508 1201 8 0.045116312317745155 1201 9 0.051088287931271736 1201 10-11 0.09763438904158761 1201 12-13 -0.02275881686885839 1201 14-15 0.02662522471461415 1201 16-17 -0.01005065027553087 1201 18-19 0.022823635551148413 1201 20-21 -0.011411788248402388 1201 22-23 -0.04344573048106781 1201 24-25 -0.009702692254180079 1201 26-27 0.03686406813994836 1201 28-29 0.11334637029838746 1201 30-31 0.049496588445443024 1201 32-33 -0.016867216684559594 1201 34-35 0.08505263310790667 1201 36-37 0.12025599221927052 1201 38-39 0.09269188148076069 1201 40-41 0.01825521798100027 1201 42-43 0.011417986888098142 1201 44-45 0.08413553378154859 1201 46-47 0.07542262370144215 1201 48-49 0.12941495592054508 1201 50-51 0.09480818135646985 1201 52-53 -0.08040304170967971 1201 54-55 0.012766567217916247 1201 56-57 0.05836206557761159 1201 58-59 0.04325883562238175 1201 60-61 -0.034813327845810704 1201 62-63 0.13792886136850058 1201 64-65 0.10017069012264557 1201 66-67 -0.009723450566788472 1201 68-69 0.061405884202585526 1201 70-71 0.08291217239612791 1201 72-73 -0.10083287772646798 1201 74-75 -0.018833311568783984 1201 76-77 0.07537310387968432 1201 78-79 0.15550418544720657 1201 80-81 -0.1501397348576674 1201 82-83 -0.07120637826173493 1201 84-85 0.03383759827842425 1201 86-87 -0.049825571042838135 1201 88-89 0.204375823697724 1201 90-91 0.33781843147806256 1202 1 0.09185074764055656 1202 2 0.1776943361793073 1202 3 0.033271278852225805 1202 4 0.13823478191685723 1202 5 0.17768569075391838 1202 6 -0.004653450423290906 1202 7 0.040710389898592325 1202 8 0.10541021296786823 1202 9 -0.03372453051173352 1202 10-11 0.018241286072196772 1202 12-13 -0.0345842818932951 1202 14-15 -0.03235975041753392 1202 16-17 -0.03926017383706437 1202 18-19 -0.03221373132123517 1202 20-21 -0.05581109733016376 1202 22-23 -0.0794463901517517 1202 24-25 0.03066568174438089 1202 26-27 0.04681120768633207 1202 28-29 0.0036361914061160405 1202 30-31 0.041309728043913196 1202 32-33 -9.62167086044019E-4 1202 34-35 -0.03673444349078636 1202 36-37 0.06922255268597866 1202 38-39 8.023528082077291E-4 1202 40-41 0.019090761153897517 1202 42-43 -0.012698650068188044 1202 44-45 -0.02718312926657518 1202 46-47 -0.09871337208011965 1202 48-49 0.0043958115698785605 1202 50-51 -0.00782303118506178 1202 52-53 -0.007369316153891248 1202 54-55 -0.12035210469829849 1202 56-57 -0.1234589634983152 1202 58-59 -0.14633373330363497 1202 60-61 -0.07647542569527843 1202 62-63 -0.08359458640403972 1202 64-65 -0.17124416686963428 1202 66-67 -0.20066198025247672 1202 68-69 -0.20081976260026124 1202 70-71 -0.178806851413718 1202 72-73 -0.0029442929442637933 1202 74-75 -0.27259424968105606 1202 76-77 -0.3501014889899281 1202 78-79 -0.3062938654675449 1202 80-81 -0.21416828251453524 1202 82-83 -0.35331831666658786 1202 84-85 0.00535622904585864 1202 86-87 -0.07018456938967432 1202 88-89 -0.12357073954296283 1202 90-91 -0.31001816882033495 1203 1 0.237528753100392 1203 2 0.18034722864767616 1203 3 0.14520023298731388 1203 4 0.17551271639124622 1203 5 0.0944076732114496 1203 6 0.032388872764407495 1203 7 0.11367663782660742 1203 8 -0.09940093929969862 1203 9 -0.04896978902338134 1203 10-11 0.032183886027638664 1203 12-13 0.015299234001027173 1203 14-15 0.06943338740373406 1203 16-17 0.046193849867549375 1203 18-19 0.022857008702104054 1203 20-21 0.013995920412419594 1203 22-23 0.09709858177500763 1203 24-25 0.0021597367127981215 1203 26-27 0.052854410640534866 1203 28-29 0.05847132014276468 1203 30-31 0.005920459684922719 1203 32-33 0.04058023620243034 1203 34-35 -0.03609279324616921 1203 36-37 0.09575979199793494 1203 38-39 0.029972737109716263 1203 40-41 -0.013460846872419552 1203 42-43 0.11777790380893549 1203 44-45 -0.09618576259764922 1203 46-47 -0.012506761707115288 1203 48-49 -0.05811706378609216 1203 50-51 0.07795539418492581 1203 52-53 0.06058913684281464 1203 54-55 0.0078428426086532 1203 56-57 0.049454759481321275 1203 58-59 0.038948514416958346 1203 60-61 -0.1602395306287434 1203 62-63 0.09995623122595276 1203 64-65 0.06542361593263735 1203 66-67 0.023804036227190295 1203 68-69 -0.05571612687346317 1203 70-71 0.1266098406073155 1203 72-73 0.14033507862366434 1203 74-75 0.1625949679096408 1203 76-77 0.09585497069354432 1203 78-79 0.12892156322045167 1203 80-81 0.042189732848953554 1203 82-83 0.3116658220017712 1203 84-85 -0.04080731308576446 1203 86-87 -0.12278449604901454 1203 88-89 0.5181013138938013 1203 90-91 -1.6389655372413898 1204 1 0.23170348198323865 1204 2 0.04134950288733563 1204 3 -0.02259039071011415 1204 4 0.07152950627636301 1204 5 -0.007560165675855046 1204 6 -0.02711284205617659 1204 7 -0.1278563898372127 1204 8 -0.02872839324817278 1204 9 -0.07640399329544323 1204 10-11 -0.0968686116140205 1204 12-13 -0.0334535905168849 1204 14-15 -0.19372319875045463 1204 16-17 0.0011192972200007034 1204 18-19 0.042461539631034384 1204 20-21 -0.01655128651413662 1204 22-23 0.012989008761671528 1204 24-25 -0.025951620591456503 1204 26-27 -0.08554587016590176 1204 28-29 -0.08672535405595028 1204 30-31 -0.05806977059098983 1204 32-33 -0.06037029758113732 1204 34-35 -0.06110669664882096 1204 36-37 -0.031177329836530987 1204 38-39 -0.06501075306732673 1204 40-41 -0.06731906203514626 1204 42-43 -0.0814554703593302 1204 44-45 -0.09666785594526317 1204 46-47 -0.08463740129678143 1204 48-49 -0.10587620631058314 1204 50-51 -0.07772149722453747 1204 52-53 -0.009516257373554993 1204 54-55 -0.11709836109246652 1204 56-57 -0.03293803271775175 1204 58-59 -0.15207638713012273 1204 60-61 -0.0018157834123257999 1204 62-63 -0.19412315385216772 1204 64-65 -0.1887435567407394 1204 66-67 -0.13267557800725172 1204 68-69 0.005937635347393666 1204 70-71 0.021879643822309447 1204 72-73 -0.1606670139584594 1204 74-75 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0.05301395363998296 2401 50-51 0.05192689094729985 2401 52-53 0.07317615903534147 2401 54-55 0.08871026045038377 2401 56-57 -0.034913272978315035 2401 58-59 0.04857705788622724 2401 60-61 0.027830364087634507 2401 62-63 0.017239670882318592 2401 64-65 0.09127315124606383 2401 66-67 0.10477129025083798 2401 68-69 0.10235576811157188 2401 70-71 0.09936626429249884 2401 72-73 0.12646307330916073 2401 74-75 -0.1779332724649123 2401 76-77 0.03278477367063459 2401 78-79 0.0077123612330964875 2401 80-81 -0.033357806775427434 2401 82-83 0.20516762053649273 2401 84-85 0.4712394972467564 2401 86-87 0.25167139765922286 2401 88-89 0.35319236057139136 2401 90-91 0.38535663181762203 2402 1 0.30334268523956354 2402 2 0.24394345755069935 2402 3 0.263034495000106 2402 4 0.32351019045729856 2402 5 0.1621616784024127 2402 6 0.20171324107228372 2402 7 0.08893862001710318 2402 8 0.23003314578644307 2402 9 0.0818313048061654 2402 10-11 0.14085412646428352 2402 12-13 0.14517980851190515 2402 14-15 0.21348840157734372 2402 16-17 0.05445181782602049 2402 18-19 0.06475329518974604 2402 20-21 0.023407806211274362 2402 22-23 0.09805582171926375 2402 24-25 0.05493506490338973 2402 26-27 0.039480035517094336 2402 28-29 -0.05422845257881903 2402 30-31 0.08713479964558246 2402 32-33 0.0995128920895354 2402 34-35 0.1028651760829078 2402 36-37 0.0688318502336287 2402 38-39 0.033443006118915264 2402 40-41 0.09098158881724316 2402 42-43 0.07435599092134026 2402 44-45 0.12118944413831656 2402 46-47 0.0374368838932142 2402 48-49 0.0807168712743973 2402 50-51 0.12181996934563699 2402 52-53 0.17058560403659584 2402 54-55 0.04042889955854889 2402 56-57 0.06534850904708378 2402 58-59 0.10510354986464421 2402 60-61 0.20313811844285823 2402 62-63 0.10653783429076213 2402 64-65 0.22939500232707388 2402 66-67 0.3012609409510105 2402 68-69 0.2384360679044093 2402 70-71 0.34116404027100344 2402 72-73 0.22824905361263603 2402 74-75 0.2925717987996137 2402 76-77 0.22874461615705144 2402 78-79 0.09582139575466186 2402 80-81 0.19536161671305052 2402 82-83 0.3305450600080917 2402 84-85 0.3143773610349996 2402 86-87 3.440983839340106E-4 2402 88-89 -0.14848785141659704 2402 90-91 0.2403273920515403 2403 1 -0.335664912473689 2403 2 -0.1870688103861795 2403 3 -0.04683929463444514 2403 4 -0.025044301471233155 2403 5 0.08516045570630126 2403 6 0.02849772229147618 2403 7 -0.08669740201671772 2403 8 0.11068950646206588 2403 9 0.1185856410268542 2403 10-11 0.06502106672417085 2403 12-13 0.04316678631771964 2403 14-15 0.0702865565557218 2403 16-17 0.0893752968795738 2403 18-19 0.0616229503206398 2403 20-21 0.060327866572102096 2403 22-23 -0.04533724346405421 2403 24-25 0.03296558829522667 2403 26-27 0.054280273682309144 2403 28-29 0.06383694572821952 2403 30-31 0.037196592086722546 2403 32-33 0.05047834322806466 2403 34-35 0.08860196806534759 2403 36-37 -0.006167642175896049 2403 38-39 0.07500649132930448 2403 40-41 0.04163346934488743 2403 42-43 -0.012114029438045293 2403 44-45 0.06207465746551577 2403 46-47 0.008245800254357505 2403 48-49 0.07952504181032083 2403 50-51 0.03396566364061471 2403 52-53 0.09260102599974118 2403 54-55 0.026002438579951104 2403 56-57 -0.03817896259187137 2403 58-59 0.13679246377889598 2403 60-61 0.004411202968000794 2403 62-63 0.16863796049307211 2403 64-65 0.05893975650760552 2403 66-67 -0.017996073376821187 2403 68-69 0.11278667413500898 2403 70-71 0.08147843661656395 2403 72-73 0.1661321472851185 2403 74-75 0.13476923720318013 2403 76-77 0.12194925578290139 2403 78-79 0.11677588865439503 2403 80-81 -0.02091798215381857 2403 82-83 0.009811197934837423 2403 84-85 0.11179239925862561 2403 86-87 0.4194807924953494 2403 88-89 0.2678885872937329 2403 90-91 0.41810516982931745 2404 1 -0.3215077223786267 2404 2 -0.35875938509415306 2404 3 -0.4560163581150789 2404 4 -0.37621628785927896 2404 5 -0.20489848882736794 2404 6 -0.15128848471330514 2404 7 -0.135716939219364 2404 8 0.013952524000927724 2404 9 -0.01592306256748799 2404 10-11 0.10061401454208863 2404 12-13 0.04645300896695659 2404 14-15 0.06079664357339709 2404 16-17 -0.05326575658308741 2404 18-19 0.023117272570047476 2404 20-21 0.042984538821080776 2404 22-23 0.056386419500618956 2404 24-25 -0.018551730800894006 2404 26-27 0.0796485622753238 2404 28-29 0.03718097585084479 2404 30-31 0.007070614296310396 2404 32-33 0.059082793052553484 2404 34-35 0.07796801924050101 2404 36-37 0.05650062154683866 2404 38-39 0.13231049414540053 2404 40-41 0.08104187176034827 2404 42-43 0.039281308901387035 2404 44-45 -0.024926794049967782 2404 46-47 0.015680623632853496 2404 48-49 0.07182710555152738 2404 50-51 0.03942607748388127 2404 52-53 0.0040901262150967455 2404 54-55 0.08126128936276444 2404 56-57 0.07805096657618549 2404 58-59 0.11215147320683627 2404 60-61 0.16202306961859847 2404 62-63 0.04233164129399114 2404 64-65 0.09486994754323064 2404 66-67 0.06542986354240554 2404 68-69 0.15206059470510525 2404 70-71 0.06008986606067168 2404 72-73 0.09519963300845546 2404 74-75 0.14386582392342007 2404 76-77 0.08649270201325265 2404 78-79 0.26640488132135687 2404 80-81 0.10641060547754932 2404 82-83 -0.15673634326660846 2404 84-85 -0.062261654795428 2404 86-87 -0.3263227457509217 2404 88-89 -0.3355537403609148 2404 90-91 -0.12349027176612992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 17.0 31 452.0 32 3323.0 33 6106.0 34 8314.0 35 10219.0 36 14529.0 37 22222.0 38 58439.0 39 364329.0 40 352926.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.781427458628915 11.267444833593977 33.11007117670494 29.84105653107217 2 27.034179109092943 18.014262505262806 29.34624633982155 25.605312045822696 3 26.7636621379914 20.21753815123492 22.29730372569167 30.721495985082008 4 26.1986309515315 20.98763670267673 21.036276454554535 31.77745589123723 5 26.86638695836247 21.574643585974627 20.959095038983154 30.59987441667975 6 27.78697453607904 23.37919027300101 23.760459330507707 25.073375860412238 7 25.679886213900744 23.109828321892884 23.676974964203996 27.53331050000238 8 24.68889586574001 24.738487006407603 24.300372468711203 26.27224465914118 9 25.10251214209943 24.88488195643591 24.508964460871756 25.503641440592904 10-11 24.620098563878624 23.56096499364948 24.67706296766705 27.141873474804846 12-13 25.161855017862322 23.319787935438757 24.289372035829302 27.228985010869618 14-15 25.646290297261427 22.958676427915652 24.161231858205014 27.233801416617908 16-17 25.476467398284647 22.57027195448556 24.025242723064995 27.9280179241648 18-19 25.82194045257565 22.766912124974432 23.88431825857796 27.526829163871962 20-21 25.81391310966183 22.88155447414363 23.905902891746226 27.398629524448314 22-23 25.4322278195596 22.38831884843901 24.19780086481241 27.98165246718898 24-25 25.630889691226766 22.75680361908296 24.19268715006731 27.419619539622964 26-27 25.75813794186063 22.793432087489713 24.22836422968428 27.220065740965378 28-29 25.47301861392167 22.4755493081025 24.34776352280241 27.703668555173415 30-31 25.794144894651154 22.733456776469215 24.304383658412153 27.16801467046748 32-33 25.788287065686667 22.764273246310832 24.357373691315 27.0900659966875 34-35 25.56357039527315 22.39704411505358 24.56197578611272 27.47740970356055 36-37 25.758158143198816 22.725652217688953 24.576285675748753 26.93990396336347 38-39 25.78571321247264 22.73732429284341 24.603803512411947 26.873158982272006 40-41 25.622016211494312 22.46267090124803 24.711220060539162 27.204092826718494 42-43 25.772631332330796 22.721059536100118 24.707901427802238 26.79840770376685 44-45 25.847048579542513 22.743064025178988 24.638362478693505 26.771524916584987 46-47 25.672768341191826 22.39965838504486 24.788940760295507 27.138632513467815 48-49 25.91145801181778 22.719636791338566 24.78054634392061 26.588358852923044 50-51 26.020793727380276 22.73553191433468 24.6513289648934 26.592345393391646 52-53 25.69784390876844 22.429302844175577 24.835070395241267 27.03778285181472 54-55 25.95373632140997 22.700893824021534 24.691523429512007 26.65384642505649 56-57 26.151627348650962 22.78375254758302 24.602108652577755 26.462511451188252 58-59 25.897959546092235 22.454345805733634 24.751587907632018 26.89610674054211 60-61 26.112244078267153 22.722714422311995 24.64143101093781 26.523610488483033 62-63 26.244333561156157 22.626088519869814 24.566482648604453 26.563095270369573 64-65 26.16240150782636 22.38792979302203 24.642073229593784 26.80759546955783 66-67 26.29868060424501 22.649450074716185 24.584988774867035 26.466880546171765 68-69 26.32435129740519 22.654091816367266 24.446506986027945 26.5750499001996 70-71 26.184466063574796 22.392400860520222 24.542336958252033 26.880796117652945 72-73 26.332024677509814 22.599734774427223 24.552235285484404 26.516005262578556 74-75 26.45544808382795 22.683251508267997 24.25899465083901 26.602305757065043 76-77 26.100183469042754 22.58507520788953 24.442473984522277 26.87226733854544 78-79 26.538137103997634 22.664857860913273 24.321915247015035 26.475089788074058 80-81 26.827409669265815 22.38935726053568 24.00090156324627 26.782331506952232 82-83 26.660105525028023 21.95180294704612 24.09989338144837 27.288198146477487 84-85 27.60045714285714 21.930057142857144 23.53645714285714 26.93302857142857 86-87 28.09225691533948 20.630762782900252 23.35498742665549 27.921992875104777 88-89 26.84086026172443 20.369365654636603 23.763536535081055 29.02623754855791 90-91 24.19695688926458 20.27155536770921 27.715553677092135 27.815934065934066 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.5 10 3.0 11 8.0 12 13.5 13 16.5 14 22.0 15 40.5 16 60.0 17 87.0 18 142.0 19 214.5 20 307.0 21 427.5 22 610.5 23 849.5 24 1139.5 25 1501.0 26 2016.0 27 2692.5 28 3419.0 29 4275.5 30 5364.5 31 6557.0 32 7806.5 33 9166.5 34 10817.5 35 12707.5 36 14729.0 37 16720.5 38 18870.5 39 21558.0 40 24408.5 41 27272.5 42 30095.5 43 33002.5 44 36050.0 45 39077.5 46 42325.5 47 45477.5 48 48437.0 49 51726.5 50 54612.5 51 56470.5 52 57835.0 53 58917.0 54 59472.5 55 59310.0 56 58417.0 57 57133.5 58 55816.5 59 54225.5 60 52094.0 61 49158.0 62 45870.5 63 42356.5 64 38494.5 65 34625.0 66 30568.0 67 26912.5 68 23502.0 69 19971.0 70 16763.0 71 13879.5 72 11187.5 73 8786.0 74 6779.5 75 5192.5 76 3943.0 77 2893.0 78 2112.0 79 1557.0 80 1135.5 81 806.5 82 551.0 83 365.0 84 264.5 85 212.0 86 152.0 87 98.5 88 78.0 89 59.0 90 34.5 91 21.5 92 14.0 93 10.5 94 11.0 95 9.5 96 7.0 97 5.0 98 4.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0013081595859555986 2 0.008324651910626537 3 0.001427083184678835 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.604486865249863E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7720.0 32-33 17415.0 34-35 20476.0 36-37 23334.0 38-39 26464.0 40-41 29285.0 42-43 31429.0 44-45 33230.0 46-47 35106.0 48-49 36167.0 50-51 36890.0 52-53 37716.0 54-55 37329.0 56-57 36852.0 58-59 36714.0 60-61 35422.0 62-63 34991.0 64-65 33845.0 66-67 32101.0 68-69 31168.0 70-71 29460.0 72-73 27436.0 74-75 25468.0 76-77 24282.0 78-79 22119.0 80-81 20488.0 82-83 18955.0 84-85 16841.0 86-87 15505.0 88-89 14088.0 90-91 12580.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.74479857714041 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77195521540104 99.51733576607651 2 0.20886193650666612 0.416657835745778 3 0.014709982755198048 0.04401732800971314 4 0.0029998363215525347 0.011968722786305895 5 5.083146753858193E-4 0.002535087245508153 6 4.4801954676263134E-4 0.0026812571670276246 7 0.0 0.0 8 0.0 0.0 9 3.506537133860254E-4 0.0031478295610813884 >10 1.66041080006956E-4 0.0016561734080824681 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017838539808485436 0.0 0.0 0.0 0.0 2 0.0017838539808485436 0.0 0.0 0.0 0.0 3 0.0017838539808485436 0.0 0.0 0.0 0.0 4 0.0017838539808485436 0.0 0.0 0.0 0.0 5 0.00190277757957178 0.0 0.0 0.0 2.378471974464725E-4 6 0.00190277757957178 0.0 0.0 0.0 2.378471974464725E-4 7 0.002259548375741489 0.0 0.0 0.0 2.378471974464725E-4 8 0.004756943948929449 0.0 0.0 0.0 3.5677079616970873E-4 9 0.004875867547652686 0.0 0.0 0.0 3.5677079616970873E-4 10-11 0.004875867547652686 0.0 0.0 0.0 4.1623259553132683E-4 12-13 0.004875867547652686 0.0 0.0 0.0 4.75694394892945E-4 14-15 0.004875867547652686 0.0 0.0 0.0 4.75694394892945E-4 16-17 0.004875867547652686 0.0 0.0 0.0 4.75694394892945E-4 18-19 0.004875867547652686 0.0 1.1892359872323624E-4 0.0 4.75694394892945E-4 20-21 0.004935329347014304 0.0 1.1892359872323624E-4 0.0 4.75694394892945E-4 22-23 0.005411023741907249 0.0 1.1892359872323624E-4 0.0 4.75694394892945E-4 24-25 0.005767794538076958 1.1892359872323624E-4 1.1892359872323624E-4 0.0 5.946179936161812E-4 26-27 0.006124565334246667 1.1892359872323624E-4 1.1892359872323624E-4 0.0 5.946179936161812E-4 28-29 0.006184027133608285 1.1892359872323624E-4 1.1892359872323624E-4 0.0 5.946179936161812E-4 30-31 0.006243488932969903 1.1892359872323624E-4 2.378471974464725E-4 0.0 5.946179936161812E-4 32-33 0.006540797929777993 1.1892359872323624E-4 3.5677079616970873E-4 0.0 5.946179936161812E-4 34-35 0.006838106926586084 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.135415923394175E-4 36-37 0.0071354159233941745 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.135415923394175E-4 38-39 0.007313801321479029 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.135415923394175E-4 40-41 0.00761111031828712 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.135415923394175E-4 42-43 0.007848957515733591 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.135415923394175E-4 44-45 0.00790841931509521 1.1892359872323624E-4 4.75694394892945E-4 0.0 7.730033917010355E-4 46-47 0.007967881114456829 1.1892359872323624E-4 4.75694394892945E-4 0.0 8.324651910626537E-4 48-49 0.007967881114456829 1.1892359872323624E-4 4.75694394892945E-4 0.0 8.324651910626537E-4 50-51 0.008324651910626537 1.1892359872323624E-4 4.75694394892945E-4 0.0 8.324651910626537E-4 52-53 0.008503037308711391 1.1892359872323624E-4 4.75694394892945E-4 0.0 8.324651910626537E-4 54-55 0.00856249910807301 1.1892359872323624E-4 4.75694394892945E-4 0.0 8.324651910626537E-4 56-57 0.00856249910807301 1.7838539808485437E-4 4.75694394892945E-4 0.0 8.919269904242718E-4 58-59 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0.009870658694028608 2.378471974464725E-4 4.75694394892945E-4 0.0 9.5138878978589E-4 >>END_MODULE