##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-1C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 560357 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.224558629587925 40.0 40.0 40.0 40.0 40.0 2 39.120582057509765 40.0 40.0 40.0 40.0 40.0 3 39.10681583347759 40.0 40.0 40.0 40.0 40.0 4 39.05812187587556 40.0 40.0 40.0 40.0 40.0 5 39.20037404725916 40.0 40.0 40.0 40.0 40.0 6 39.249863926032866 40.0 40.0 40.0 40.0 40.0 7 39.29038809187714 40.0 40.0 40.0 40.0 40.0 8 39.30274450038101 40.0 40.0 40.0 40.0 40.0 9 39.320725894385184 40.0 40.0 40.0 40.0 40.0 10-11 39.32957025610459 40.0 40.0 40.0 40.0 40.0 12-13 39.32961308594343 40.0 40.0 40.0 40.0 40.0 14-15 39.34297956481315 40.0 40.0 40.0 40.0 40.0 16-17 39.47615181036375 40.0 40.0 40.0 40.0 40.0 18-19 39.49232721283039 40.0 40.0 40.0 40.0 40.0 20-21 39.503266667499474 40.0 40.0 40.0 40.0 40.0 22-23 39.50635398504882 40.0 40.0 40.0 40.0 40.0 24-25 39.50702141670399 40.0 40.0 40.0 40.0 40.0 26-27 39.49230936706421 40.0 40.0 40.0 40.0 40.0 28-29 39.49587138199398 40.0 40.0 40.0 40.0 40.0 30-31 39.49411802045229 40.0 40.0 40.0 40.0 40.0 32-33 39.48505240680524 40.0 40.0 40.0 40.0 40.0 34-35 39.489567851636586 40.0 40.0 40.0 40.0 40.0 36-37 39.490213506444434 40.0 40.0 40.0 40.0 40.0 38-39 39.47365147714808 40.0 40.0 40.0 40.0 40.0 40-41 39.46445915944924 40.0 40.0 40.0 40.0 40.0 42-43 39.46318617110192 40.0 40.0 40.0 40.0 40.0 44-45 39.443176756505125 40.0 40.0 40.0 40.0 40.0 46-47 39.44576074080328 40.0 40.0 40.0 40.0 40.0 48-49 39.442617448286995 40.0 40.0 40.0 40.0 40.0 50-51 39.44781636853248 40.0 40.0 40.0 40.0 40.0 52-53 39.4428414997331 40.0 40.0 40.0 40.0 40.0 54-55 39.41412878705043 40.0 40.0 40.0 40.0 40.0 56-57 39.41750519618877 40.0 40.0 40.0 40.0 40.0 58-59 39.40627260522834 40.0 40.0 40.0 40.0 40.0 60-61 39.37134905730855 40.0 40.0 40.0 40.0 40.0 62-63 39.35290740697583 40.0 40.0 40.0 40.0 40.0 64-65 39.34225597897933 40.0 40.0 40.0 40.0 40.0 66-67 39.34697316882914 40.0 40.0 40.0 40.0 40.0 68-69 39.33583476080406 40.0 40.0 40.0 40.0 40.0 70-71 39.321238852338254 40.0 40.0 40.0 40.0 40.0 72-73 39.328423347914054 40.0 40.0 40.0 40.0 40.0 74-75 39.32185585934077 40.0 40.0 40.0 40.0 40.0 76-77 39.316034065868244 40.0 40.0 40.0 40.0 40.0 78-79 39.31567520301873 40.0 40.0 40.0 40.0 40.0 80-81 39.29482542354443 40.0 40.0 40.0 40.0 40.0 82-83 39.28448010717061 40.0 40.0 40.0 40.0 40.0 84-85 39.281820464159715 40.0 40.0 40.0 40.0 40.0 86-87 39.29534012964427 40.0 40.0 40.0 40.0 40.0 88-89 39.31784706381707 40.0 40.0 40.0 40.0 40.0 90-91 39.42699228208204 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15759499862350168 1101 2 0.12834856957633178 1101 3 0.19172229778387617 1101 4 0.13141629079460415 1101 5 -0.0617425406702381 1101 6 0.0018824752119286359 1101 7 0.03858352785499619 1101 8 0.14595909955754394 1101 9 0.04107094525454613 1101 10-11 -0.06229869581017056 1101 12-13 0.005704850323624555 1101 14-15 -0.04852613778167836 1101 16-17 -0.009302575486685782 1101 18-19 0.021797992914542874 1101 20-21 -0.08598952374136815 1101 22-23 0.09038713539412413 1101 24-25 0.03770902317113922 1101 26-27 0.019885864523679686 1101 28-29 0.00455008240087551 1101 30-31 0.02567029048913838 1101 32-33 -0.011712581718178683 1101 34-35 0.007959021749663009 1101 36-37 0.030183310899317917 1101 38-39 -0.03365902522791231 1101 40-41 7.751904093709072E-4 1101 42-43 0.06763601278561993 1101 44-45 0.09272999534805138 1101 46-47 0.015712979580229103 1101 48-49 0.15061263006433023 1101 50-51 0.13976752371165446 1101 52-53 0.043859266156815124 1101 54-55 0.08000456068511141 1101 56-57 0.06407732334425731 1101 58-59 0.11305389750827288 1101 60-61 -0.010772685457425268 1101 62-63 -0.13089613695665037 1101 64-65 0.11360548415291305 1101 66-67 -0.011082692513355141 1101 68-69 -0.11221551338086755 1101 70-71 -0.06100951356236095 1101 72-73 -0.01971704537552199 1101 74-75 0.07116384054576486 1101 76-77 -0.14281993567804818 1101 78-79 -0.12504255765698957 1101 80-81 -0.11128456911411888 1101 82-83 0.2324482644000483 1101 84-85 -0.17159569522667795 1101 86-87 -0.05927040117211391 1101 88-89 0.0034633630940206217 1101 90-91 0.541576911695131 1102 1 0.3548917352082199 1102 2 0.17065796384242304 1102 3 0.06777175807857105 1102 4 -0.04985897510064774 1102 5 0.02427932575914582 1102 6 0.027068323072711564 1102 7 0.07101021703486765 1102 8 0.010978208998821515 1102 9 0.10099316437214156 1102 10-11 -0.08768361284269588 1102 12-13 -0.03339185532141897 1102 14-15 -0.05337575061612654 1102 16-17 0.072826649186851 1102 18-19 -0.089897800609954 1102 20-21 -0.07315675743538463 1102 22-23 -0.010935475386077087 1102 24-25 0.1118694849156796 1102 26-27 0.016745128615319516 1102 28-29 -0.06990538546564551 1102 30-31 -0.040327136002169084 1102 32-33 -0.11133665123075076 1102 34-35 -0.005955311741807634 1102 36-37 0.10286290633157336 1102 38-39 -0.04973883947318569 1102 40-41 -0.01121162760500738 1102 42-43 -0.08318456241941163 1102 44-45 -0.08693379979429494 1102 46-47 -0.08476280111878509 1102 48-49 -0.00569863025037165 1102 50-51 -0.056544277459948944 1102 52-53 -0.0061210882504383335 1102 54-55 -0.0261676481573474 1102 56-57 -0.16090750908288243 1102 58-59 -0.07000710377014485 1102 60-61 -0.011661736357197583 1102 62-63 -0.04338002316161749 1102 64-65 -0.1261621617405595 1102 66-67 -0.029082520332991635 1102 68-69 -0.018723922530874404 1102 70-71 0.013952289248152283 1102 72-73 -0.15094276963891673 1102 74-75 -0.26780730041576817 1102 76-77 0.024872244336854976 1102 78-79 0.018272660235716387 1102 80-81 -0.012862048079192334 1102 82-83 0.09254535898812577 1102 84-85 -0.11184099109405565 1102 86-87 -0.3649479032685008 1102 88-89 -0.4846358129215176 1102 90-91 -1.0676184906037207 1103 1 0.2924776009784438 1103 2 0.1442211529405668 1103 3 0.25618645296983544 1103 4 0.09517164118679489 1103 5 0.19376126991077314 1103 6 -0.018980055990056144 1103 7 0.02381271360023618 1103 8 0.1644048469064856 1103 9 0.06250213148076966 1103 10-11 -0.00757157321643831 1103 12-13 -0.04770725752653249 1103 14-15 0.12281791390905994 1103 16-17 0.05248297759548137 1103 18-19 0.01871067001207649 1103 20-21 -0.007316101137092801 1103 22-23 0.020880905659865334 1103 24-25 0.04371899595862061 1103 26-27 -0.14534396095846347 1103 28-29 0.11718642632817478 1103 30-31 0.0812113005863182 1103 32-33 0.0850120553731486 1103 34-35 -0.11099559586839547 1103 36-37 -0.07805684196686968 1103 38-39 -0.05941015877046851 1103 40-41 0.03942235534615435 1103 42-43 -0.07081927650053643 1103 44-45 -0.14552123535825956 1103 46-47 -0.0013436988881991851 1103 48-49 -0.106183150052928 1103 50-51 -0.10559819167119144 1103 52-53 0.019100065072805705 1103 54-55 0.04135850345990377 1103 56-57 -0.05723983886921502 1103 58-59 -0.04658819487946886 1103 60-61 -0.09524665860856629 1103 62-63 -0.15866334965501494 1103 64-65 -0.0805017374293513 1103 66-67 0.014736108190099628 1103 68-69 -0.1262944995211015 1103 70-71 -0.12171950442464663 1103 72-73 -0.12371729665270692 1103 74-75 -0.17746780501268233 1103 76-77 -0.11063778510661848 1103 78-79 -0.379496431897266 1103 80-81 -0.4876704394001763 1103 82-83 -0.09443961815588153 1103 84-85 -0.3771413143752298 1103 86-87 0.16074253171645125 1103 88-89 0.12041643075085773 1103 90-91 0.12981220581278308 1104 1 0.22562068747606645 1104 2 0.2140861423770204 1104 3 0.1304338826527527 1104 4 0.012012896815910779 1104 5 0.15673370764316275 1104 6 0.1713005948481907 1104 7 0.06198704968436175 1104 8 -0.0555860671163515 1104 9 -0.029036883087670162 1104 10-11 -0.04605024542878766 1104 12-13 0.05675722115449133 1104 14-15 -0.03509725129252672 1104 16-17 -0.0515823355840368 1104 18-19 -0.024650612257474336 1104 20-21 -0.04014250163461952 1104 22-23 -0.06833148126162314 1104 24-25 -0.08028915495680877 1104 26-27 -0.09124490616472514 1104 28-29 0.032506001316150446 1104 30-31 0.007617090911956836 1104 32-33 -0.09912237034095028 1104 34-35 0.012825272502773544 1104 36-37 0.02043119655970571 1104 38-39 -0.04898555991825759 1104 40-41 0.05155903376808624 1104 42-43 -0.016926357035494277 1104 44-45 -8.998952284002826E-4 1104 46-47 -0.1167266745372828 1104 48-49 -0.05510358211749633 1104 50-51 -0.037254941921588625 1104 52-53 0.04900657918189211 1104 54-55 0.04061076271224806 1104 56-57 0.06834206439661017 1104 58-59 0.08596581311180529 1104 60-61 -0.008838004654322162 1104 62-63 -0.16082980136511082 1104 64-65 0.13457717299141336 1104 66-67 -0.07453015232496796 1104 68-69 0.06182010722677944 1104 70-71 -0.02672694632352801 1104 72-73 -0.06509599784275366 1104 74-75 0.20740942212120927 1104 76-77 0.05821081570918807 1104 78-79 0.005001242258607874 1104 80-81 -0.03167767977798519 1104 82-83 -0.10048596607837368 1104 84-85 0.01868087622028014 1104 86-87 -0.03215017686525812 1104 88-89 0.12975507128974328 1104 90-91 0.541576911695131 1201 1 0.21676447418329303 1201 2 0.12508457901875403 1201 3 0.1898837495560315 1201 4 0.14778844927292312 1201 5 0.16033365693964896 1201 6 0.15130651025783237 1201 7 0.08580924937168533 1201 8 0.19510054293139234 1201 9 0.044584615201088695 1201 10-11 0.05512438591443214 1201 12-13 0.07329079110421333 1201 14-15 0.09919099816875132 1201 16-17 -0.01785182474615965 1201 18-19 0.049456794680452276 1201 20-21 0.11076804892100967 1201 22-23 -0.030295397262804613 1201 24-25 0.08383388556374172 1201 26-27 0.0571051170743786 1201 28-29 0.046888897331697876 1201 30-31 0.09019198422331698 1201 32-33 0.0728377925215824 1201 34-35 -0.0021197843877089895 1201 36-37 0.03064002072661509 1201 38-39 0.13541652731739617 1201 40-41 -0.11129874860775857 1201 42-43 0.01231860544321961 1201 44-45 -0.014254158701504593 1201 46-47 -0.12008838207886896 1201 48-49 -0.0108417471943838 1201 50-51 0.04035722211587256 1201 52-53 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18-19 -0.0028167496246425117 1202 20-21 0.07520174277571101 1202 22-23 -0.007461350076617634 1202 24-25 -0.030758499321059674 1202 26-27 0.024293563956540254 1202 28-29 0.07273345202104053 1202 30-31 0.009936435172903657 1202 32-33 -0.06629114720026763 1202 34-35 0.02406015278437934 1202 36-37 -0.05206823144894912 1202 38-39 -0.07415254292499895 1202 40-41 0.055827838702896315 1202 42-43 -0.04419807871079229 1202 44-45 -0.04329505817533885 1202 46-47 0.021483428236187763 1202 48-49 0.07445289168491342 1202 50-51 -0.011605091055955086 1202 52-53 -0.03617522399179052 1202 54-55 -0.12799225723115626 1202 56-57 -0.12331400960371042 1202 58-59 -0.07624379096780842 1202 60-61 -0.09145291351250506 1202 62-63 0.020918919856548257 1202 64-65 -0.07273338831079457 1202 66-67 0.12983799287971465 1202 68-69 -0.0341077241688339 1202 70-71 -0.04094015885573299 1202 72-73 -0.18346908618635638 1202 74-75 0.08502395594587142 1202 76-77 -0.23118315565199055 1202 78-79 0.21246685385912656 1202 80-81 0.03563864922017501 1202 82-83 -0.41475024723999354 1202 84-85 -0.26494098864939275 1202 86-87 0.08722274963655252 1202 88-89 0.013367682397756653 1202 90-91 -0.7329607838951944 1203 1 0.15994480039649517 1203 2 0.14215270001052716 1203 3 -8.752263567046725E-4 1203 4 0.03852217648596934 1203 5 0.07528593689723095 1203 6 0.07284365109508428 1203 7 0.06086353407602729 1203 8 0.01050224974348879 1203 9 0.07136843583432295 1203 10-11 -0.012415885761569712 1203 12-13 0.03998270647057467 1203 14-15 -0.05051359204485095 1203 16-17 0.013794895447745148 1203 18-19 0.12002691031949553 1203 20-21 0.027815970953021463 1203 22-23 0.03575015139144 1203 24-25 0.08651024773109128 1203 26-27 0.01969159387082442 1203 28-29 0.028538142395575505 1203 30-31 0.09342381778389353 1203 32-33 -0.04868069464312441 1203 34-35 -4.3114856638482024E-4 1203 36-37 0.01615849370519129 1203 38-39 0.011411756090019765 1203 40-41 0.07402501433683994 1203 42-43 0.007076556676643975 1203 44-45 -0.017411522233011567 1203 46-47 0.060222267684501674 1203 48-49 -0.007365533161269866 1203 50-51 0.002758791608385991 1203 52-53 0.030863222308781246 1203 54-55 0.0457416679813889 1203 56-57 -0.02408246010832471 1203 58-59 0.04369005143477267 1203 60-61 0.06056554119858504 1203 62-63 -0.17913493488956078 1203 64-65 -0.07195037413021765 1203 66-67 0.04542388541577225 1203 68-69 0.15312226961391673 1203 70-71 -0.13071071577001447 1203 72-73 -0.22913061940155188 1203 74-75 0.13706448662625803 1203 76-77 -0.1756230378277266 1203 78-79 -0.26746592272390046 1203 80-81 0.1325512315984696 1203 82-83 -0.0998482847528237 1203 84-85 -0.15444291744889682 1203 86-87 -0.522472074267796 1203 88-89 -0.08363227620660751 1203 90-91 -1.2943205242023055 1204 1 0.3449028489877435 1204 2 0.05751469797149866 1204 3 -0.0540431668228436 1204 4 0.06975190746236137 1204 5 -0.11568983844097147 1204 6 -0.07333412258256544 1204 7 -0.2574004085284898 1204 8 -0.13767197101530115 1204 9 -0.09239699582639105 1204 10-11 -0.1295770515542074 1204 12-13 -0.1259949020980642 1204 14-15 -0.1945425831057861 1204 16-17 -0.027536290049148704 1204 18-19 -0.125203024086332 1204 20-21 -0.12042826738112922 1204 22-23 -0.04342742595314064 1204 24-25 -0.07982501818193555 1204 26-27 -0.04804851792074771 1204 28-29 -0.22643085826911147 1204 30-31 -0.16080715978919358 1204 32-33 -0.20798986092332683 1204 34-35 -0.0862790341151296 1204 36-37 -0.1255248881074138 1204 38-39 -0.15661593640425053 1204 40-41 -0.125199089597416 1204 42-43 -0.10178297513802903 1204 44-45 -0.12198660298443542 1204 46-47 -0.1983644644912843 1204 48-49 -0.1015701517580041 1204 50-51 -0.18103814021741016 1204 52-53 -0.15432698498005237 1204 54-55 -0.047563677777588964 1204 56-57 -0.026335823726292062 1204 58-59 -0.03699872784618918 1204 60-61 -0.13142168244129948 1204 62-63 0.00944497559056856 1204 64-65 -0.19385890331147237 1204 66-67 -0.04079672654427213 1204 68-69 0.03458580386711674 1204 70-71 -0.031168270691438238 1204 72-73 0.14552408466628464 1204 74-75 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0.09272718206617014 2401 54-55 0.13113751672073448 2401 56-57 0.09896734303544719 2401 58-59 0.08548771095761509 2401 60-61 0.03725581654859411 2401 62-63 0.06387847363170351 2401 64-65 0.07911352207883482 2401 66-67 0.12579731479110734 2401 68-69 0.03377552872211709 2401 70-71 -0.023429359967984453 2401 72-73 0.1354880417429385 2401 74-75 -0.026507416820621188 2401 76-77 0.20060882121387635 2401 78-79 0.0719938989768707 2401 80-81 -0.1480962754050381 2401 82-83 -0.21941545700494203 2401 84-85 -0.21689482878628752 2401 86-87 0.20421385462942965 2401 88-89 0.5114989507153638 2401 90-91 0.10571484272961129 2402 1 0.27650159420976195 2402 2 0.21689152892395924 2402 3 0.24813469432070434 2402 4 0.16419999766905136 2402 5 0.1139848383726374 2402 6 0.19203127518002816 2402 7 -0.0012032584218246711 2402 8 0.12771369201971083 2402 9 0.13511406513810442 2402 10-11 0.015660579626185722 2402 12-13 0.20137924907013627 2402 14-15 0.13094272546577201 2402 16-17 0.16223368122780357 2402 18-19 0.015461807977146691 2402 20-21 0.026607012670041286 2402 22-23 0.05391144575993678 2402 24-25 -0.020381609849216886 2402 26-27 0.03381364604080517 2402 28-29 0.05350288621083621 2402 30-31 0.011004799940131704 2402 32-33 0.09144129792121447 2402 34-35 0.06485072323408758 2402 36-37 0.10386537464086842 2402 38-39 -0.060464018853416235 2402 40-41 0.17310771969177097 2402 42-43 0.028753357338665353 2402 44-45 -0.047612190365434515 2402 46-47 -0.1580566299814663 2402 48-49 0.07055531774302182 2402 50-51 0.02447036514155343 2402 52-53 -0.13141291161628033 2402 54-55 -0.044796829805143545 2402 56-57 0.0038728923394373282 2402 58-59 -0.00948998587320915 2402 60-61 0.06471119128043767 2402 62-63 0.10288161996126632 2402 64-65 -0.012118290522344921 2402 66-67 -0.04490963094077216 2402 68-69 -0.05981539132802283 2402 70-71 0.01170837798047586 2402 72-73 0.19217295046839666 2402 74-75 0.22024766836736376 2402 76-77 0.3936304304929692 2402 78-79 0.36298975400355715 2402 80-81 0.19916857734665427 2402 82-83 0.34937460671510223 2402 84-85 0.23586106948322794 2402 86-87 0.28593337919173223 2402 88-89 0.2006901827637435 2402 90-91 0.3987197688379922 2403 1 -0.3101784509426295 2403 2 -0.10584771602775334 2403 3 -0.009986464590625133 2403 4 0.0036581689972834397 2403 5 0.07154159241753888 2403 6 0.12360032070868954 2403 7 -0.002457491458322636 2403 8 0.07719318530956087 2403 9 -0.03380604021747047 2403 10-11 0.13717067620212475 2403 12-13 0.08225219526354266 2403 14-15 0.15116096201411722 2403 16-17 0.047847628299166445 2403 18-19 0.029559387307379836 2403 20-21 0.05354793829401672 2403 22-23 0.127008147127043 2403 24-25 0.04426156085190058 2403 26-27 0.12045606420209509 2403 28-29 0.08882208851862572 2403 30-31 0.015645462175179148 2403 32-33 0.12464647195041323 2403 34-35 0.06371055784658353 2403 36-37 0.1681603166191863 2403 38-39 0.10832980856581997 2403 40-41 0.0224754613779794 2403 42-43 0.05308683064618691 2403 44-45 0.07554036333409186 2403 46-47 0.0785305109838319 2403 48-49 3.2083456957821E-5 2403 50-51 0.036065793157945336 2403 52-53 0.06594584888676991 2403 54-55 0.04227176790232079 2403 56-57 -0.019814589305418906 2403 58-59 0.10682547123071373 2403 60-61 0.05754995940979768 2403 62-63 0.09535595545438014 2403 64-65 0.10370384468470917 2403 66-67 0.16425872345743642 2403 68-69 0.053820768143467035 2403 70-71 0.1891037154969979 2403 72-73 0.24922542706193695 2403 74-75 0.13642182463106423 2403 76-77 0.29009118757550567 2403 78-79 0.20087175579629957 2403 80-81 0.3084603578095084 2403 82-83 0.2098484783722654 2403 84-85 0.39193548056960736 2403 86-87 0.3661782993347771 2403 88-89 0.25681856616484566 2403 90-91 0.265714842729615 2404 1 -0.5165750394287727 2404 2 -0.29945112754160874 2404 3 -0.41766237077398927 2404 4 -0.2624399120261742 2404 5 -0.27302130736924113 2404 6 -0.1544380588222296 2404 7 -0.17303318442207427 2404 8 0.06354712987246103 2404 9 -0.0013966585543556675 2404 10-11 0.10987856532792506 2404 12-13 0.10869142767292317 2404 14-15 0.05222706009514155 2404 16-17 0.126312447062503 2404 18-19 -0.028436348300296288 2404 20-21 0.029667341279093762 2404 22-23 0.016986825165425046 2404 24-25 0.03146838387961992 2404 26-27 0.07013623477779163 2404 28-29 0.04276665141840397 2404 30-31 0.09411028093850149 2404 32-33 0.012222466823409661 2404 34-35 0.09159180657416499 2404 36-37 0.03306849805847634 2404 38-39 0.05531075423335352 2404 40-41 0.06118913316493746 2404 42-43 0.035706817703456295 2404 44-45 0.08055364632540574 2404 46-47 0.09103505167579584 2404 48-49 0.07615091786158246 2404 50-51 0.03818487677079219 2404 52-53 0.10808402312638776 2404 54-55 0.12125551257108214 2404 56-57 0.0590808133720131 2404 58-59 0.0070249629940875025 2404 60-61 0.06495200841884241 2404 62-63 0.07471754430375199 2404 64-65 0.12233396603347302 2404 66-67 -0.020128916490747883 2404 68-69 -0.011230598199958308 2404 70-71 0.03485160643361951 2404 72-73 -0.038955198560110205 2404 74-75 -0.006729977147188038 2404 76-77 0.205825057270566 2404 78-79 -0.007061786439365392 2404 80-81 0.04460308662191892 2404 82-83 -0.015859428662707842 2404 84-85 0.1333637850045406 2404 86-87 -0.02303061003541984 2404 88-89 -0.05527829635367709 2404 90-91 -0.05156764866254093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 314.0 32 2292.0 33 4092.0 34 5365.0 35 6835.0 36 9532.0 37 14664.0 38 37589.0 39 246073.0 40 233590.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.06322488306395 14.596082084558015 26.487956612753838 30.8527364196242 2 26.44720286272655 19.595577408732744 27.042057450852656 26.915162277688044 3 25.907914696172035 20.16793075756224 21.865441242080845 32.058713304184884 4 26.27646304052595 22.025244620839928 20.657545100712582 31.04074723792154 5 26.818260501787254 21.815735325872613 20.767653478050597 30.598350694289532 6 26.795953294060748 24.64625229987312 23.518042961897503 25.03975144416863 7 24.996207774686493 24.50973218858692 23.585678415724264 26.908381621002324 8 24.020936652883787 25.702543200138482 24.26024837737371 26.01627176960402 9 23.8756721161688 25.842810922322734 24.590395051725952 25.691121909782517 10-11 23.842835906395386 24.907603545596825 24.32627771224416 26.92328283576363 12-13 24.322083957191577 24.594142662624005 24.07295706130199 27.010816318882426 14-15 24.621625142543056 24.17708710696931 23.9810513654688 27.220236385018836 16-17 24.623766634484802 23.99398954595017 23.83498376927566 27.547260050289367 18-19 24.755914533056604 24.320745524727986 23.665895134708766 27.25744480750664 20-21 24.7558253042257 24.443613624885565 23.493237346905634 27.307323723983103 22-23 24.727539764828492 24.071529400007496 23.667322796003262 27.53360803916075 24-25 24.76367744134543 24.31262570111554 23.628597483390053 27.29509937414898 26-27 24.911351156494877 24.355544768781332 23.501892543503516 27.23121153122027 28-29 24.89136389837193 24.003090886702587 23.69444836059869 27.411096854326793 30-31 24.97894195832649 24.307672265488367 23.62399974302051 27.08938603316463 32-33 25.128628872781178 24.353914254658466 23.491410713687742 27.026046158872607 34-35 25.10991211655633 24.072626633650636 23.571466231797324 27.24599501799571 36-37 25.072652009445946 24.40528768108448 23.64674662261847 26.8753136868511 38-39 25.17009393618902 24.418623955437198 23.57281739230639 26.838464716067396 40-41 25.202330933130572 24.140566859834014 23.670421969176026 26.986680237859385 42-43 25.287848984472344 24.35579151004 23.671157882823167 26.685201622664483 44-45 25.35703252835684 24.34730971637159 23.472180226243406 26.823477529028168 46-47 25.328705500879135 24.098714406411982 23.65800470394812 26.91457538876076 48-49 25.387854849114934 24.386351154631114 23.486532701158108 26.739261295095844 50-51 25.5388424276296 24.367715227182494 23.490823936413683 26.602618408774227 52-53 25.508843337037586 24.197003574823604 23.586070237919905 26.708082850218908 54-55 25.695300160787717 24.44243657422559 23.399023890493602 26.463239374493092 56-57 25.74120183402666 24.327118695533358 23.40054049130052 26.531138979139463 58-59 25.710700708259555 24.278232736173315 23.39681413394438 26.614252421622748 60-61 25.815286959266338 24.34585912430518 23.327254554700243 26.511599361728244 62-63 26.004463015039903 24.336145488218172 23.139366752815 26.520024743926925 64-65 26.041670974652504 24.142986530134532 23.17172527595234 26.643617219260623 66-67 26.1708232678938 24.2421149331428 23.096412734056912 26.490649064906492 68-69 26.21294526433401 24.21130687696813 23.014332131682252 26.561415727015603 70-71 26.22670078669766 24.039536027176826 23.014817629179333 26.71894555694618 72-73 26.384693800498376 24.312529890004782 22.815512597850436 26.487263711646403 74-75 26.64886591812849 24.132660913636542 22.607547183317884 26.61092598491709 76-77 26.69743975095271 24.04181616247528 22.495179663340256 26.76556442323175 78-79 27.134806688729853 24.222660470885806 22.09790819713437 26.544624643249975 80-81 27.291179583813147 24.241313931442 21.874818252666596 26.59268823207826 82-83 27.72204285930966 23.50833856976736 21.792955010003396 26.97666356091959 84-85 28.58426797985283 23.22167506132155 20.693916527795984 27.500140431029642 86-87 29.471906582185685 22.080693302612975 19.997905430172278 28.449494685029066 88-89 29.014263074484948 21.4770206022187 20.05705229793978 29.45166402535658 90-91 26.972308633790604 22.703860472232137 21.872609516038555 28.4512213779387 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 0.5 9 0.0 10 0.0 11 1.0 12 3.5 13 4.0 14 3.0 15 6.0 16 11.0 17 20.5 18 35.0 19 56.5 20 83.0 21 113.5 22 162.5 23 241.5 24 328.0 25 431.0 26 591.5 27 839.0 28 1188.0 29 1645.5 30 2213.5 31 2872.5 32 3687.0 33 4593.5 34 5850.0 35 7445.0 36 9080.0 37 10835.0 38 12934.5 39 15478.0 40 18216.5 41 21060.5 42 23796.0 43 26386.5 44 28856.0 45 31365.5 46 34062.5 47 36180.5 48 37702.0 49 38868.5 50 39455.5 51 39567.5 52 39451.5 53 39065.0 54 38157.0 55 37110.5 56 35663.5 57 33881.0 58 32164.5 59 30254.0 60 28458.5 61 26972.0 62 25537.5 63 23790.5 64 21774.5 65 19848.0 66 17715.0 67 15713.0 68 13938.0 69 12024.5 70 10258.5 71 8512.0 72 6799.5 73 5353.5 74 4122.0 75 3178.0 76 2460.5 77 1796.5 78 1321.0 79 965.0 80 655.5 81 437.0 82 285.0 83 171.0 84 100.0 85 67.5 86 48.0 87 33.5 88 23.0 89 15.5 90 11.0 91 5.5 92 2.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014276612944961872 2 0.009279798414225217 3 0.001249203632684164 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 1.897536807470223E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5059.0 32-33 11598.0 34-35 13089.0 36-37 14686.0 38-39 16467.0 40-41 17688.0 42-43 18815.0 44-45 19855.0 46-47 20744.0 48-49 21530.0 50-51 21876.0 52-53 21985.0 54-55 22025.0 56-57 22197.0 58-59 22117.0 60-61 21688.0 62-63 21728.0 64-65 21498.0 66-67 20958.0 68-69 20706.0 70-71 20195.0 72-73 19529.0 74-75 18670.0 76-77 18008.0 78-79 17386.0 80-81 16925.0 82-83 16024.0 84-85 15403.0 86-87 14686.0 88-89 14045.0 90-91 13177.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.60006622526365 #Duplication Level Percentage of deduplicated Percentage of total 1 99.62009356991298 99.22167916930294 2 0.360246794937568 0.7176120926644148 3 0.017686032900367677 0.05284590144416437 4 0.0019736022490908322 0.007862836588471046 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.92288309060117E-4 0.0 0.0 0.0 0.0 2 8.92288309060117E-4 0.0 0.0 0.0 0.0 3 8.92288309060117E-4 0.0 0.0 0.0 0.0 4 8.92288309060117E-4 0.0 0.0 0.0 0.0 5 8.92288309060117E-4 0.0 0.0 0.0 0.0 6 0.0010707459708721404 0.0 0.0 0.0 0.0 7 0.0010707459708721404 0.0 0.0 0.0 0.0 8 0.005532187516172725 0.0 0.0 0.0 0.0 9 0.005532187516172725 0.0 0.0 0.0 1.784576618120234E-4 10-11 0.005532187516172725 0.0 0.0 0.0 1.784576618120234E-4 12-13 0.005532187516172725 0.0 0.0 0.0 1.784576618120234E-4 14-15 0.005532187516172725 0.0 0.0 0.0 1.784576618120234E-4 16-17 0.005710645177984749 0.0 0.0 0.0 1.784576618120234E-4 18-19 0.005710645177984749 0.0 0.0 0.0 1.784576618120234E-4 20-21 0.005978331670702784 0.0 0.0 0.0 1.784576618120234E-4 22-23 0.006067560501608796 0.0 0.0 0.0 1.784576618120234E-4 24-25 0.006067560501608796 0.0 0.0 0.0 2.676864927180351E-4 26-27 0.006067560501608796 0.0 0.0 0.0 3.569153236240468E-4 28-29 0.006067560501608796 0.0 0.0 0.0 3.569153236240468E-4 30-31 0.006424475825232843 0.0 0.0 0.0 3.569153236240468E-4 32-33 0.0065137046561388546 0.0 0.0 0.0 3.569153236240468E-4 34-35 0.00678139114885689 0.0 0.0 0.0 3.569153236240468E-4 36-37 0.007138306472480936 0.0 0.0 0.0 3.569153236240468E-4 38-39 0.007138306472480936 0.0 0.0 0.0 3.569153236240468E-4 40-41 0.007495221796104982 0.0 0.0 0.0 3.569153236240468E-4 42-43 0.008030594781541053 0.0 0.0 0.0 5.353729854360702E-4 44-45 0.008119823612447064 0.0 0.0 0.0 5.353729854360702E-4 46-47 0.008209052443353076 0.0 0.0 0.0 5.353729854360702E-4 48-49 0.008209052443353076 0.0 0.0 0.0 6.246018163420818E-4 50-51 0.008209052443353076 0.0 0.0 0.0 7.138306472480936E-4 52-53 0.008209052443353076 0.0 0.0 0.0 8.030594781541054E-4 54-55 0.0083875101051651 0.0 0.0 0.0 8.92288309060117E-4 56-57 0.008655196597883136 0.0 0.0 0.0 8.92288309060117E-4 58-59 0.00892288309060117 0.0 0.0 0.0 8.92288309060117E-4 60-61 0.009101340752413194 0.0 0.0 0.0 8.92288309060117E-4 62-63 0.009190569583319205 0.0 0.0 0.0 0.0010707459708721404 64-65 0.009725942568755277 0.0 0.0 0.0 0.0010707459708721404 66-67 0.010172086723285335 0.0 0.0 0.0 0.0010707459708721404 68-69 0.010261315554191346 0.0 0.0 0.0 0.0010707459708721404 70-71 0.010350544385097358 0.0 0.0 0.0 0.0010707459708721404 72-73 0.010350544385097358 0.0 0.0 0.0 0.0010707459708721404 74-75 0.01043977321600337 0.0 0.0 0.0 0.0010707459708721404 76-77 0.010529002046909382 0.0 0.0 0.0 0.0010707459708721404 78-79 0.010529002046909382 0.0 0.0 0.0 0.0010707459708721404 80 0.010529002046909382 0.0 0.0 0.0 0.0010707459708721404 >>END_MODULE