##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-1B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 678314 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25547165472038 40.0 40.0 40.0 40.0 40.0 2 39.12469151454931 40.0 40.0 40.0 40.0 40.0 3 39.12270423432216 40.0 40.0 40.0 40.0 40.0 4 39.063884867480255 40.0 40.0 40.0 40.0 40.0 5 39.209604991198766 40.0 40.0 40.0 40.0 40.0 6 39.24136609298938 40.0 40.0 40.0 40.0 40.0 7 39.2762584879569 40.0 40.0 40.0 40.0 40.0 8 39.30105526349154 40.0 40.0 40.0 40.0 40.0 9 39.33601842214667 40.0 40.0 40.0 40.0 40.0 10-11 39.323867707286006 40.0 40.0 40.0 40.0 40.0 12-13 39.331622228053675 40.0 40.0 40.0 40.0 40.0 14-15 39.34739663341756 40.0 40.0 40.0 40.0 40.0 16-17 39.47858366479241 40.0 40.0 40.0 40.0 40.0 18-19 39.49262141132278 40.0 40.0 40.0 40.0 40.0 20-21 39.50696285201249 40.0 40.0 40.0 40.0 40.0 22-23 39.50829261964223 40.0 40.0 40.0 40.0 40.0 24-25 39.51246767721144 40.0 40.0 40.0 40.0 40.0 26-27 39.49733899049703 40.0 40.0 40.0 40.0 40.0 28-29 39.500364138142515 40.0 40.0 40.0 40.0 40.0 30-31 39.49301908034634 40.0 40.0 40.0 40.0 40.0 32-33 39.49680454707635 40.0 40.0 40.0 40.0 40.0 34-35 39.49314212239186 40.0 40.0 40.0 40.0 40.0 36-37 39.4942309609414 40.0 40.0 40.0 40.0 40.0 38-39 39.478148891371525 40.0 40.0 40.0 40.0 40.0 40-41 39.46990828547196 40.0 40.0 40.0 40.0 40.0 42-43 39.46532676018714 40.0 40.0 40.0 40.0 40.0 44-45 39.44642477494857 40.0 40.0 40.0 40.0 40.0 46-47 39.45040769976828 40.0 40.0 40.0 40.0 40.0 48-49 39.450975976771936 40.0 40.0 40.0 40.0 40.0 50-51 39.450763257031966 40.0 40.0 40.0 40.0 40.0 52-53 39.43989908197529 40.0 40.0 40.0 40.0 40.0 54-55 39.423721801808284 40.0 40.0 40.0 40.0 40.0 56-57 39.41507146274992 40.0 40.0 40.0 40.0 40.0 58-59 39.40302341053318 40.0 40.0 40.0 40.0 40.0 60-61 39.374483078922474 40.0 40.0 40.0 40.0 40.0 62-63 39.33543652473904 40.0 40.0 40.0 40.0 40.0 64-65 39.32882196527567 40.0 40.0 40.0 40.0 40.0 66-67 39.333009481636054 40.0 40.0 40.0 40.0 40.0 68-69 39.3302087477874 40.0 40.0 40.0 40.0 40.0 70-71 39.31758502592618 40.0 40.0 40.0 40.0 40.0 72-73 39.31170460771609 40.0 40.0 40.0 40.0 40.0 74-75 39.326823003920296 40.0 40.0 40.0 40.0 40.0 76-77 39.31249061903241 40.0 40.0 40.0 40.0 40.0 78-79 39.31162786329431 40.0 40.0 40.0 40.0 40.0 80-81 39.31135182633524 40.0 40.0 40.0 40.0 40.0 82-83 39.283001525985874 40.0 40.0 40.0 40.0 40.0 84-85 39.30094422023814 40.0 40.0 40.0 40.0 40.0 86-87 39.313254048740106 40.0 40.0 40.0 40.0 40.0 88-89 39.34166641492784 40.0 40.0 40.0 40.0 40.0 90-91 39.40813986834617 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16402347365264802 1101 2 0.09709591895062175 1101 3 0.1684985056866921 1101 4 0.04078154334846573 1101 5 0.15796204860490803 1101 6 0.10212221559883972 1101 7 0.14841815763369937 1101 8 0.02062656316499556 1101 9 0.1274719629049983 1101 10-11 0.11472164648971983 1101 12-13 0.06182454124164849 1101 14-15 0.05954268273224983 1101 16-17 0.07520357765332619 1101 18-19 -0.0038263938605425096 1101 20-21 0.09626586298553974 1101 22-23 0.07241726960074146 1101 24-25 0.1345801561326141 1101 26-27 0.049997717459234536 1101 28-29 0.0590128485528254 1101 30-31 0.08688754809931964 1101 32-33 0.08182563017346212 1101 34-35 0.04684060232935394 1101 36-37 0.10191706341969109 1101 38-39 0.12378162513513047 1101 40-41 0.11037958152361682 1101 42-43 0.08968366829888907 1101 44-45 0.08512376846102399 1101 46-47 0.1535995135129795 1101 48-49 0.06847893851162468 1101 50-51 0.1384377205830134 1101 52-53 0.15014244227975126 1101 54-55 0.1325931530452351 1101 56-57 0.13356097296353653 1101 58-59 0.16326364410528527 1101 60-61 0.0880458186887978 1101 62-63 0.15716103902926193 1101 64-65 0.03090544527368877 1101 66-67 0.0604440772693593 1101 68-69 0.2554024244733313 1101 70-71 0.05804793969654298 1101 72-73 0.22948122973068052 1101 74-75 0.20757098203384317 1101 76-77 0.03919116405610623 1101 78-79 0.276697772297986 1101 80-81 0.2287707318748744 1101 82-83 0.42238758973419266 1101 84-85 0.36357614022031726 1101 86-87 0.2478452124352799 1101 88-89 0.7058033838833069 1101 90-91 0.07186457444338146 1102 1 0.2814420126375339 1102 2 0.19902131823008062 1102 3 0.12329667670869782 1102 4 -0.06445986293788764 1102 5 0.030442542250639804 1102 6 0.167863112118269 1102 7 0.191043979598291 1102 8 0.08431212109211828 1102 9 0.11991315910164246 1102 10-11 -0.07027314519897487 1102 12-13 0.023964787836725066 1102 14-15 0.004557494121748107 1102 16-17 0.11467284731499205 1102 18-19 0.07145249729796177 1102 20-21 -0.0317957893621994 1102 22-23 0.0662343321001515 1102 24-25 0.02217109819426355 1102 26-27 -0.013954032198185473 1102 28-29 0.15301172195652413 1102 30-31 -0.07959032202840177 1102 32-33 -0.009097935527613288 1102 34-35 -0.10158083463984013 1102 36-37 -0.07645787879290822 1102 38-39 -0.003987826160170016 1102 40-41 0.06769766112904563 1102 42-43 0.10926886278885206 1102 44-45 0.0838975201438501 1102 46-47 0.05668127762077546 1102 48-49 0.03978345041516462 1102 50-51 -0.0035611874686267697 1102 52-53 0.038610830547519015 1102 54-55 0.04932177451645714 1102 56-57 0.031124870775890656 1102 58-59 -0.0839383820687587 1102 60-61 -0.13551209170942258 1102 62-63 -0.1163279667839916 1102 64-65 -0.013931027952246211 1102 66-67 -0.09677944596995047 1102 68-69 -0.04512117076467348 1102 70-71 -0.032469244346280846 1102 72-73 0.10153826870731564 1102 74-75 -0.08350361248426452 1102 76-77 -0.08688151907986708 1102 78-79 -0.012262962278526857 1102 80-81 -0.0043600040644662386 1102 82-83 -0.22044759412784032 1102 84-85 -0.28393001208212354 1102 86-87 0.06355578475673695 1102 88-89 -0.4234722700402358 1102 90-91 -0.08429158171277606 1103 1 0.31782632190416393 1103 2 0.24336469514878445 1103 3 0.16650304396281967 1103 4 0.017404832912028212 1103 5 -0.021859902320137792 1103 6 0.029147933039247675 1103 7 -0.029535895330646156 1103 8 0.04565379249633139 1103 9 0.12673521708913427 1103 10-11 0.07526409186753824 1103 12-13 0.05125301978669938 1103 14-15 0.04776386137586286 1103 16-17 0.08283657670668987 1103 18-19 -0.009275188887379215 1103 20-21 0.03301376151898694 1103 22-23 0.10261864136678156 1103 24-25 0.018760069200510543 1103 26-27 0.12476114688083584 1103 28-29 -0.015265708401635436 1103 30-31 0.038658683088137025 1103 32-33 0.0583349671683564 1103 34-35 0.10028128945550208 1103 36-37 0.14501464377980255 1103 38-39 0.003033791252974538 1103 40-41 0.04135318059249471 1103 42-43 -0.009369399106361698 1103 44-45 -0.011485097597329741 1103 46-47 0.006786063942612941 1103 48-49 -0.06195832684868918 1103 50-51 0.054649925519406395 1103 52-53 0.027499221767143922 1103 54-55 0.07265627971123934 1103 56-57 -0.015835089351369902 1103 58-59 0.21634987189541732 1103 60-61 -0.023028442079102263 1103 62-63 -0.2631357883206107 1103 64-65 -0.11821421086112593 1103 66-67 -0.023643021481454696 1103 68-69 -0.0032778283349657045 1103 70-71 0.05356862658860706 1103 72-73 0.11790729829006352 1103 74-75 0.03918626511189416 1103 76-77 0.09571922235376462 1103 78-79 0.10483647907592797 1103 80-81 0.17701849861081342 1103 82-83 0.09981758623634818 1103 84-85 0.17994391015312772 1103 86-87 0.022611831954371553 1103 88-89 -0.11800613992621578 1103 90-91 0.008372510951311085 1104 1 0.30873894777793964 1104 2 0.21927177740108306 1104 3 0.1958439855423464 1104 4 0.19078108955170592 1104 5 0.03142848939553744 1104 6 0.13940740933719553 1104 7 0.03671154694155376 1104 8 0.06372285915064424 1104 9 -0.13178932481999084 1104 10-11 0.031102238635035917 1104 12-13 0.003103240365952331 1104 14-15 0.024416309723200413 1104 16-17 0.020026272999530192 1104 18-19 -0.012661973279200822 1104 20-21 -0.03474181932417508 1104 22-23 -0.054966297886501536 1104 24-25 -0.012388027707025628 1104 26-27 0.0016893309508247967 1104 28-29 0.06257624849830279 1104 30-31 -0.009724217504953003 1104 32-33 -0.05637075504817801 1104 34-35 0.05258523373138502 1104 36-37 0.009514842287558167 1104 38-39 0.01917921478993634 1104 40-41 0.031170676268551745 1104 42-43 0.01849665971697334 1104 44-45 -0.004015885444800915 1104 46-47 0.09512291330954525 1104 48-49 0.03198361351113732 1104 50-51 -0.03489895450108094 1104 52-53 0.017643874474465804 1104 54-55 0.018703958243463603 1104 56-57 -0.012862758524988749 1104 58-59 -0.012632727651386233 1104 60-61 0.16716682392636528 1104 62-63 0.03619676674715322 1104 64-65 0.16650833166761458 1104 66-67 -0.013029170765477716 1104 68-69 0.08971095620740499 1104 70-71 -0.052132216612342575 1104 72-73 0.11763948568244587 1104 74-75 -0.09559681792370611 1104 76-77 -0.04744274973383256 1104 78-79 0.03496455580931013 1104 80-81 0.0067450795086614335 1104 82-83 -0.1458578494390892 1104 84-85 -0.12660800999479704 1104 86-87 -0.2322964096205311 1104 88-89 -0.12143675948586719 1104 90-91 -0.21620750673215383 1201 1 0.25745079720535813 1201 2 0.13017211218991065 1201 3 0.2272299513744116 1201 4 0.23514997141693783 1201 5 0.06481450016570278 1201 6 0.2279570322316573 1201 7 0.10532902636365549 1201 8 0.11276290648544318 1201 9 0.06367821863889844 1201 10-11 0.16341318031286534 1201 12-13 -0.04878887824067135 1201 14-15 0.05479026959348943 1201 16-17 0.0693251637774921 1201 18-19 0.06066741548777799 1201 20-21 0.06711906730449613 1201 22-23 0.030393918415541066 1201 24-25 0.06212592931717609 1201 26-27 0.06742657789316553 1201 28-29 0.1788682562576156 1201 30-31 0.17103838201420984 1201 32-33 0.09847382252738868 1201 34-35 0.11644385865969298 1201 36-37 0.04026145633940814 1201 38-39 0.10399739264933316 1201 40-41 0.014122807843826024 1201 42-43 0.10298476163807635 1201 44-45 0.08094357478810821 1201 46-47 -0.00976153714659489 1201 48-49 -0.07291019584985037 1201 50-51 -0.11139907731539012 1201 52-53 0.06403859281522983 1201 54-55 0.034632227444106434 1201 56-57 0.03621044273405971 1201 58-59 0.15666893647946978 1201 60-61 0.005011668289377269 1201 62-63 0.026119599977981522 1201 64-65 -0.2223225859586364 1201 66-67 -0.12861835336439498 1201 68-69 0.04213677644382585 1201 70-71 -0.08277070524840724 1201 72-73 0.07007043875394459 1201 74-75 -0.08361778894216343 1201 76-77 0.040484311670581974 1201 78-79 -0.2101572661337059 1201 80-81 -0.06481076979735434 1201 82-83 -0.07073002535603479 1201 84-85 -0.1999235180161918 1201 86-87 -0.6377659327745349 1201 88-89 -0.8248908215096975 1201 90-91 -2.804492986124707 1202 1 0.15879583422268695 1202 2 0.08998000626566238 1202 3 0.11655802396089143 1202 4 0.1923546824452984 1202 5 0.11081943581022102 1202 6 0.22084261098770241 1202 7 0.028799937093509698 1202 8 -0.1457501936608523 1202 9 0.03889478112504463 1202 10-11 0.11515203447492439 1202 12-13 0.07262561245697441 1202 14-15 0.20135645008438985 1202 16-17 0.016080494716888438 1202 18-19 0.05184000049464288 1202 20-21 0.06835918493243298 1202 22-23 0.003210634586430672 1202 24-25 0.02244216522324649 1202 26-27 -0.009807687892767092 1202 28-29 -0.02423913095404373 1202 30-31 0.17106143472511803 1202 32-33 0.08652790381008657 1202 34-35 -0.050465082244635084 1202 36-37 0.15609085966288205 1202 38-39 0.09778193420299175 1202 40-41 0.061843844019790595 1202 42-43 0.0962302272567257 1202 44-45 0.02326862436831334 1202 46-47 0.038133155442167777 1202 48-49 -0.017598975284350615 1202 50-51 0.08864678850154206 1202 52-53 0.01158911520159478 1202 54-55 -0.012011956014063685 1202 56-57 -0.05391706033766752 1202 58-59 -0.00610039103293758 1202 60-61 0.06912564220532857 1202 62-63 -0.05579249783009743 1202 64-65 -0.06714205589085509 1202 66-67 -0.36308107760229547 1202 68-69 -0.12398253187745922 1202 70-71 -0.19298988441064324 1202 72-73 -0.07093451103818893 1202 74-75 -0.20443481809252262 1202 76-77 -0.14665794214569416 1202 78-79 -0.04417325612114098 1202 80-81 -0.2031313224139808 1202 82-83 0.12834043722704536 1202 84-85 0.03150618647359238 1202 86-87 -0.22203983516408954 1202 88-89 -0.4131324533623939 1202 90-91 -0.35829415571534895 1203 1 0.23452891964338107 1203 2 -0.01910390253723193 1203 3 0.0707740061093034 1203 4 0.03561802036830386 1203 5 -0.05404012144504122 1203 6 0.10534490171456667 1203 7 -0.05347372230598779 1203 8 -0.17478132712166428 1203 9 -0.12560793218018773 1203 10-11 0.08425686090672713 1203 12-13 -0.03384887496135036 1203 14-15 -0.0888715426801312 1203 16-17 -0.0036046139737919702 1203 18-19 0.08466729623963687 1203 20-21 0.038562715105108225 1203 22-23 -0.07183810865378604 1203 24-25 0.09018382428936889 1203 26-27 0.02158401525062459 1203 28-29 0.05494618491741221 1203 30-31 -0.031082446164127475 1203 32-33 0.014473784814036605 1203 34-35 0.026696187902139457 1203 36-37 0.05566143196483608 1203 38-39 0.01204900591170599 1203 40-41 -0.06853291697221664 1203 42-43 0.003565287049909216 1203 44-45 0.036849817123083994 1203 46-47 -0.13718018461739234 1203 48-49 -0.01308505277837213 1203 50-51 -0.13359215218881815 1203 52-53 0.015643568534080998 1203 54-55 -0.068572281781897 1203 56-57 -0.07909448093461435 1203 58-59 0.059462953761567405 1203 60-61 -0.1233822640738893 1203 62-63 -0.04658909111797982 1203 64-65 -0.14639380563357918 1203 66-67 0.069592279182217 1203 68-69 0.10321257609493273 1203 70-71 0.04223195914126876 1203 72-73 0.016974716147288404 1203 74-75 -0.07736075142120313 1203 76-77 -0.30514061159523465 1203 78-79 -0.30372428582003863 1203 80-81 0.046152347674848215 1203 82-83 -0.05223918052629983 1203 84-85 -0.005964377741257465 1203 86-87 -0.07720798786544236 1203 88-89 0.7058033838833069 1203 90-91 -0.3916274890486804 1204 1 0.2827109273603199 1204 2 0.08779193026060028 1204 3 -0.11927851588909988 1204 4 0.07398792489193085 1204 5 -0.04503341268080874 1204 6 0.014232514919612527 1204 7 -0.049016064061689235 1204 8 0.0025870894214747864 1204 9 -0.11385602793644267 1204 10-11 -0.137864639411724 1204 12-13 -0.19355859024584277 1204 14-15 -0.0014194165245200452 1204 16-17 -0.13505983604274263 1204 18-19 -0.09806698928696989 1204 20-21 -0.10929627803761122 1204 22-23 -0.06749411955231466 1204 24-25 -0.04376412209762748 1204 26-27 -0.03266186995496412 1204 28-29 -0.10142656990281296 1204 30-31 -0.16927442135112614 1204 32-33 -0.09811068328729533 1204 34-35 -0.04435098689892669 1204 36-37 -0.17080740289988228 1204 38-39 -0.0019304881768960058 1204 40-41 0.010182572263602196 1204 42-43 -0.08616845218803348 1204 44-45 -0.04348432376912115 1204 46-47 -0.24708074822274284 1204 48-49 0.0022365834874520374 1204 50-51 -0.0012944810174246868 1204 52-53 -0.14915896036880127 1204 54-55 0.015399493398795983 1204 56-57 -0.0856199992576876 1204 58-59 -0.1084483953922728 1204 60-61 -0.022648619180834828 1204 62-63 -0.025537364783431826 1204 64-65 0.06838650112415223 1204 66-67 -0.15494448790823157 1204 68-69 -0.2569321620194245 1204 70-71 -0.06493110063546226 1204 72-73 -0.12608193409606372 1204 74-75 -0.00999983549535699 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0.10398667719039878 2401 50-51 0.11627383146785064 2401 52-53 0.07004554449473943 2401 54-55 0.15178421857152813 2401 56-57 0.13187191132116993 2401 58-59 0.12491297174591409 2401 60-61 0.1477461285761521 2401 62-63 0.08893268283681266 2401 64-65 0.15481684957643438 2401 66-67 0.07045005493590395 2401 68-69 -0.10373343612034347 2401 70-71 0.0846375320182986 2401 72-73 0.12137915819855039 2401 74-75 0.07480742201637725 2401 76-77 0.26683876873855183 2401 78-79 0.2804732851968623 2401 80-81 0.2133270056216503 2401 82-83 -0.027429012567772304 2401 84-85 -0.01167063487540787 2401 86-87 0.21653376669252822 2401 88-89 0.29811107619099886 2401 90-91 0.5394459572789998 2402 1 0.3004139572000355 2402 2 0.24808140863890316 2402 3 0.32920095815722306 2402 4 0.26709038902566107 2402 5 0.14974068715865485 2402 6 0.1405837113113222 2402 7 0.06357879361993923 2402 8 0.15483233061098645 2402 9 0.2154643604130726 2402 10-11 0.09103121462594288 2402 12-13 0.150727014830224 2402 14-15 0.0798893519182613 2402 16-17 0.10332968565539602 2402 18-19 0.05546459596390463 2402 20-21 0.019772345665977298 2402 22-23 0.034192295859348576 2402 24-25 -0.038312958832847244 2402 26-27 -4.4414959718608316E-4 2402 28-29 -0.01681734023128456 2402 30-31 0.05442713051800041 2402 32-33 0.08541046209123238 2402 34-35 -4.769774541841798E-4 2402 36-37 0.027413106384273078 2402 38-39 0.06402308648686983 2402 40-41 0.12397310130081962 2402 42-43 0.015521290049896663 2402 44-45 0.05232796476330037 2402 46-47 0.0745674659391824 2402 48-49 0.13964464705856017 2402 50-51 0.079613842832849 2402 52-53 0.008219820390294785 2402 54-55 0.14954945573617096 2402 56-57 -0.03201671367880721 2402 58-59 0.2132062633960885 2402 60-61 0.1978700695186646 2402 62-63 0.09979077194401498 2402 64-65 0.1559103303282754 2402 66-67 0.11826965101922582 2402 68-69 0.10325257591583892 2402 70-71 0.15241374241330874 2402 72-73 0.2891462030872134 2402 74-75 0.06482972274064736 2402 76-77 -0.0633229267866966 2402 78-79 0.1584105003629972 2402 80-81 0.1831692619761398 2402 82-83 0.42221988842587876 2402 84-85 0.28919677345704997 2402 86-87 0.3157393333837675 2402 88-89 0.5153271934071171 2402 90-91 0.4889926659900752 2403 1 -0.22622753866475875 2403 2 -0.0749936243228575 2403 3 -0.03295354262186834 2403 4 -0.021657063928905984 2403 5 0.13393761871804344 2403 6 0.0688888773187557 2403 7 0.047887795178127135 2403 8 0.054942003390564764 2403 9 0.04879829305120609 2403 10-11 0.06161164133096264 2403 12-13 0.08243173735209552 2403 14-15 0.12077242755052708 2403 16-17 0.038981429632386266 2403 18-19 0.08404514252988804 2403 20-21 0.03906816251842571 2403 22-23 0.04650291554052188 2403 24-25 0.03151414173049716 2403 26-27 0.050737080176276095 2403 28-29 0.07379215275685169 2403 30-31 0.0644076531117932 2403 32-33 0.06818868674927359 2403 34-35 0.10310505567269246 2403 36-37 0.006329797820981753 2403 38-39 -0.03607573505342998 2403 40-41 0.027939281988878406 2403 42-43 0.10778188155686763 2403 44-45 0.017142022195002937 2403 46-47 0.07393279534816344 2403 48-49 0.03165063417258551 2403 50-51 0.054699115260604 2403 52-53 0.019478314342606495 2403 54-55 0.029455901270495133 2403 56-57 -0.03722950490747934 2403 58-59 0.04255334263756083 2403 60-61 0.017220629901878226 2403 62-63 0.14647501055769396 2403 64-65 0.049932117027267964 2403 66-67 0.08516646809989226 2403 68-69 0.17840365214108544 2403 70-71 0.15246902144441776 2403 72-73 0.2338126218219898 2403 74-75 0.2635283578367478 2403 76-77 0.27999946318573166 2403 78-79 -0.02196759723479147 2403 80-81 0.13478652335892605 2403 82-83 0.3445774671201889 2403 84-85 0.3197179998862012 2403 86-87 0.42348709704016585 2403 88-89 0.06544639043748646 2403 90-91 -0.03924653666772571 2404 1 -0.488903316046418 2404 2 -0.33832606167490553 2404 3 -0.4975705341996459 2404 4 -0.3970590765858262 2404 5 -0.12683655627181167 2404 6 -0.11697300952352663 2404 7 -0.07215247682292869 2404 8 -0.03959934850372804 2404 9 -0.016682613115975187 2404 10-11 0.08745893310963027 2404 12-13 0.12196868007531236 2404 14-15 0.10709161852658156 2404 16-17 0.03542874593264145 2404 18-19 0.0495209793292517 2404 20-21 0.05654569225085737 2404 22-23 0.028082837071416122 2404 24-25 0.04065727709912892 2404 26-27 -0.007354338209268008 2404 28-29 -0.026591940274300896 2404 30-31 -0.004432593424532172 2404 32-33 6.146352522620191E-4 2404 34-35 -0.0016281110061910908 2404 36-37 0.02873205962816172 2404 38-39 0.062131417845179726 2404 40-41 0.05604146307999969 2404 42-43 0.025602599454700226 2404 44-45 -0.021655412638530436 2404 46-47 0.0033548884304437365 2404 48-49 0.0490744375068104 2404 50-51 0.05677608202788775 2404 52-53 0.01695614244037813 2404 54-55 -0.010044101699641317 2404 56-57 0.05874599174698858 2404 58-59 -0.1173189602029936 2404 60-61 0.05397116013559611 2404 62-63 0.13508737866362708 2404 64-65 0.04474276953051515 2404 66-67 0.28550936141287764 2404 68-69 0.2409975712932706 2404 70-71 0.09739379902956813 2404 72-73 0.0031764184629992087 2404 74-75 0.05953100304412118 2404 76-77 0.24108003635932818 2404 78-79 0.12818750708413518 2404 80-81 0.1953961391135195 2404 82-83 0.3140280776001063 2404 84-85 0.28898871743577814 2404 86-87 0.0415565331450054 2404 88-89 -0.07793742311331187 2404 90-91 0.23059473317353962 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 397.0 32 2641.0 33 4859.0 34 6363.0 35 7966.0 36 11365.0 37 17581.0 38 46320.0 39 298288.0 40 282523.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.238671746001792 14.732627258574327 27.6693046185781 30.359396376845783 2 26.117373003177224 19.582758951147415 27.992303893021166 26.3075641526542 3 25.852310840435617 20.304937468948243 22.423164868797574 31.41958682181857 4 25.97454866035494 22.14726513089808 21.424443546793963 30.453742661953022 5 26.369351067499714 22.08976963471195 21.495796931804446 30.045082365983895 6 26.56232954059624 24.58728553442801 24.032969981454016 24.817414943521733 7 24.860757702185126 24.435143606058553 24.004811930757732 26.699286760998593 8 23.923728538700363 25.61439097527104 24.657901797692514 25.80397868833608 9 23.84736272581725 25.70830028570839 24.980024000684047 25.464312987790315 10-11 23.68040465035367 24.819257747886674 24.818004640918513 26.682332960841144 12-13 23.897560716718218 24.65399505243884 24.604239334585458 26.844204896257484 14-15 24.28712955946656 24.233983081581687 24.438165805217054 27.040721553734702 16-17 24.26553189230946 24.056041892103067 24.292805396910573 27.385620818676898 18-19 24.33880179385948 24.287203271641157 24.23324595983571 27.14074897466365 20-21 24.436986410423494 24.430794587757294 24.009898070804965 27.122320931014247 22-23 24.392537969141134 24.172138567094294 24.064297655658 27.37102580810657 24-25 24.503695928434325 24.27850523503864 24.05132431292882 27.166474523598218 26-27 24.5444587609868 24.335853306875578 24.008128978614625 27.111558953523 28-29 24.52345079122648 24.10483935168668 24.128722096256304 27.242987760830527 30-31 24.703621555519682 24.246089750535702 24.025174200425027 27.02511449351959 32-33 24.78419802415487 24.350537925444858 23.933252233910277 26.932011816489997 34-35 24.76101173927775 24.09775669583959 24.012784791635276 27.12844677324739 36-37 24.8006397124436 24.35895220380308 24.022679000087976 26.817729083665338 38-39 24.893525491095193 24.344808086634647 23.973271394348203 26.788395027921965 40-41 24.863770392485595 24.177713557845767 24.015010010589577 26.943506039079058 42-43 24.916553570773576 24.384347877727183 23.988293920069587 26.710804631429653 44-45 25.073130382067443 24.301655065625617 23.97279659345716 26.652417958849778 46-47 25.07407130405767 24.102850282957174 24.029977543690084 26.793100869295074 48-49 25.240580589693018 24.32215647600263 23.91624943104233 26.521013503262026 50-51 25.294579732914375 24.2497928966498 23.84180197597494 26.613825394460882 52-53 25.165453342157512 24.107693009881558 23.896026837673155 26.83082681028777 54-55 25.502569154760902 24.390306430761004 23.656366852593145 26.450757561884952 56-57 25.52406412202125 24.324891553716817 23.63860071173817 26.512443612523757 58-59 25.478995546268692 24.21394332180969 23.67471056409211 26.632350567829505 60-61 25.520169786122963 24.347127175154178 23.644653996228683 26.488049042494172 62-63 25.798329885336663 24.233916561272437 23.46519477691322 26.50255877647768 64-65 25.758486501192074 24.050248983935987 23.5322447524039 26.659019762468038 66-67 25.92326445769314 24.24166763088503 23.3862536970162 26.448814214405626 68-69 26.002018373064878 24.105786228554788 23.28060126746604 26.611594130914295 70-71 26.143812370194013 23.974469378450937 23.211336811711668 26.670381439643382 72-73 26.257502164016643 24.15473941213517 23.05044387504206 26.537314548806123 74-75 26.364713688234264 24.197940839574965 22.925077325798263 26.512268146392508 76-77 26.52774201977428 23.91858190802999 22.76309671568609 26.790579356509642 78-79 26.748105232473403 23.970175630374584 22.779113831802945 26.502605305349075 80-81 26.997597372539513 23.78490973615183 22.38466286066965 26.83283003063901 82-83 27.597669045894968 23.418502810247922 21.986828040412398 26.99700010344471 84-85 28.164562677213706 22.88472304997201 21.578082389698576 27.372631883115712 86-87 29.436084008599305 21.856992024016993 20.53020569640381 28.17671827097989 88-89 28.817617072214315 21.23913895940396 20.473448496481126 29.469795471900607 90-91 27.36769488518774 22.178969153444367 21.36592096186065 29.087414999507246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 2.5 13 6.0 14 12.0 15 20.5 16 24.5 17 29.0 18 41.0 19 67.5 20 109.5 21 164.5 22 227.0 23 311.5 24 450.5 25 644.0 26 926.0 27 1278.0 28 1717.0 29 2316.5 30 3032.5 31 3956.0 32 5113.5 33 6329.0 34 8037.5 35 10051.5 36 12273.5 37 14836.5 38 17590.0 39 20674.0 40 24028.0 41 27778.5 42 31324.0 43 34558.5 44 37676.0 45 40475.5 46 43137.5 47 45502.5 48 47256.5 49 48326.5 50 48708.5 51 48647.5 52 48352.0 53 47722.5 54 46517.5 55 44653.0 56 42463.5 57 40334.5 58 38154.5 59 35909.0 60 33851.0 61 31638.5 62 29586.5 63 27614.5 64 25147.0 65 22688.5 66 20209.0 67 17928.5 68 15820.5 69 13580.5 70 11531.0 71 9685.5 72 7914.5 73 6176.0 74 4684.5 75 3599.0 76 2727.0 77 1966.0 78 1419.0 79 1026.5 80 708.5 81 468.0 82 313.0 83 200.0 84 138.5 85 98.5 86 57.5 87 35.0 88 26.0 89 15.5 90 8.0 91 4.0 92 2.0 93 1.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014742434919521048 2 0.007223793110565313 3 0.0016216678411473152 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6103.0 32-33 14517.0 34-35 16535.0 36-37 18860.0 38-39 20969.0 40-41 22710.0 42-43 24540.0 44-45 25958.0 46-47 26895.0 48-49 27633.0 50-51 28300.0 52-53 28564.0 54-55 28442.0 56-57 28142.0 58-59 27890.0 60-61 27139.0 62-63 26510.0 64-65 25948.0 66-67 25127.0 68-69 24164.0 70-71 23716.0 72-73 22770.0 74-75 21528.0 76-77 20678.0 78-79 19212.0 80-81 18603.0 82-83 17288.0 84-85 16319.0 86-87 15397.0 88-89 14243.0 90-91 13614.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.72448194583569 #Duplication Level Percentage of deduplicated Percentage of total 1 99.73657035183223 99.46177809390863 2 0.25214689788661787 0.50290437531985 3 0.009715895342795761 0.02906737889100795 4 0.0015668549383683064 0.006250151880522147 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0011793947935616839 0.0 0.0 0.0 0.0 2 0.0011793947935616839 0.0 0.0 0.0 0.0 3 0.0011793947935616839 0.0 0.0 0.0 0.0 4 0.0011793947935616839 0.0 0.0 0.0 0.0 5 0.0011793947935616839 0.0 0.0 0.0 1.4742434919521048E-4 6 0.0011793947935616839 0.0 0.0 0.0 1.4742434919521048E-4 7 0.0019165165395377362 0.0 0.0 0.0 1.4742434919521048E-4 8 0.007518641808955734 0.0 0.0 0.0 1.4742434919521048E-4 9 0.007813490507346156 0.0 0.0 0.0 1.4742434919521048E-4 10-11 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 12-13 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 14-15 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 16-17 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 18-19 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 20-21 0.007960914856541365 0.0 0.0 0.0 1.4742434919521048E-4 22-23 0.007960914856541365 0.0 7.371217459760524E-5 0.0 2.9484869839042097E-4 24-25 0.008255763554931787 0.0 1.4742434919521048E-4 0.0 4.422730475856314E-4 26-27 0.008403187904126997 0.0 1.4742434919521048E-4 0.0 4.422730475856314E-4 28-29 0.008403187904126997 0.0 1.4742434919521048E-4 0.0 5.896973967808419E-4 30-31 0.008698036602517417 0.0 1.4742434919521048E-4 0.0 5.896973967808419E-4 32-33 0.008845460951712628 0.0 1.4742434919521048E-4 0.0 5.896973967808419E-4 34-35 0.009066597475505443 1.4742434919521048E-4 1.4742434919521048E-4 0.0 5.896973967808419E-4 36-37 0.00928773399929826 1.4742434919521048E-4 1.4742434919521048E-4 0.0 5.896973967808419E-4 38-39 0.009361446173895865 1.4742434919521048E-4 1.4742434919521048E-4 0.0 5.896973967808419E-4 40-41 0.009435158348493471 1.4742434919521048E-4 1.4742434919521048E-4 0.0 6.634095713784472E-4 42-43 0.009435158348493471 1.4742434919521048E-4 1.4742434919521048E-4 0.0 8.845460951712628E-4 44-45 0.009435158348493471 1.4742434919521048E-4 1.4742434919521048E-4 0.0 9.582582697688681E-4 46-47 0.009508870523091077 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0010319704443664734 48-49 0.00958258269768868 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 50-51 0.009656294872286286 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 52-53 0.009803719221481497 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 54-55 0.010319704443664734 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 56-57 0.010319704443664734 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 58-59 0.01039341661826234 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 60-61 0.010614553142055155 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 62-63 0.010614553142055155 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 64-65 0.010761977491250366 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 66-67 0.010761977491250366 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 68-69 0.010761977491250366 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 70-71 0.010909401840445575 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 72-73 0.01098311401504318 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 74-75 0.011204250538835996 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 76-77 0.011204250538835996 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 78-79 0.011204250538835996 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 80 0.011204250538835996 1.4742434919521048E-4 1.4742434919521048E-4 0.0 0.0011793947935616839 >>END_MODULE