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"GCGTT":49747, "GCGTC":49274, "GCGTG":43487, "GCGCA":53540, "GCGCT":54049, "GCGCC":77110, "GCGCG":57898, "GCGGA":76289, "GCGGT":63638, "GCGGC":94192, "GCGGG":75522, "GGAAA":108020, "GGAAT":76449, "GGAAC":76024, "GGAAG":513693, "GGATA":59956, "GGATT":70094, "GGATC":55225, "GGATG":76634, "GGACA":58107, "GGACT":41971, "GGACC":47592, "GGACG":53102, "GGAGA":168618, "GGAGT":46020, "GGAGC":72754, "GGAGG":126117, "GGTAA":34317, "GGTAT":35939, "GGTAC":27616, "GGTAG":57612, "GGTTA":40669, "GGTTT":100364, "GGTTC":78843, "GGTTG":96572, "GGTCA":59298, "GGTCT":70568, "GGTCC":44722, "GGTCG":58119, "GGTGA":81383, "GGTGT":49162, "GGTGC":58961, "GGTGG":98465, "GGCAA":90750, "GGCAT":66879, "GGCAC":52158, "GGCAG":98622, "GGCTA":51749, "GGCTT":102342, "GGCTC":67630, "GGCTG":105164, "GGCCA":78537, "GGCCT":68512, "GGCCC":60021, "GGCCG":77618, "GGCGA":101895, "GGCGT":61427, "GGCGC":73874, "GGCGG":117306, "GGGAA":75295, "GGGAT":61210, "GGGAC":44977, "GGGAG":93285, "GGGTA":36839, "GGGTT":86349, "GGGTC":55477, "GGGTG":68800, "GGGCA":72257, "GGGCT":72249, "GGGCC":54864, "GGGCG":74820, "GGGGA":78233, "GGGGT":69298, "GGGGC":64369, "GGGGG":129287 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1307128, "total_bases": 79734808, "q20_bases": 77921598, "q30_bases": 74635892, "total_cycles": 61, "quality_curves": { "A":[39.2523,39.1149,38.9026,38.9691,38.8773,39.0683,39.0012,38.7964,39.0456,39.031,39.0393,39.0447,39.0773,39.0687,39.0584,39.0608,39.0738,39.0547,39.0693,39.0444,38.6507,39.028,39.0697,39.0818,38.676,39.0212,39.0549,38.6069,38.8957,38.7797,39.021,39.0561,38.9816,39.0456,39.0168,38.99,38.5955,38.9925,38.9588,38.943,38.9004,38.8413,38.7939,38.7374,38.6458,38.4969,38.1643,38.0361,38.2959,38.0335,37.8854,37.7154,37.6149,37.1926,37.1321,36.8588,36.6804,36.4162,36.2799,35.8085,35.5628], 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"GC":[0.576535,0.524428,0.573579,0.564392,0.56522,0.513938,0.515902,0.496937,0.493167,0.502617,0.507936,0.50544,0.513071,0.515909,0.513856,0.518225,0.518797,0.514793,0.517355,0.519072,0.517014,0.51891,0.519937,0.517017,0.521192,0.520896,0.515979,0.516714,0.520218,0.518663,0.519042,0.520275,0.516405,0.51889,0.5216,0.516911,0.518527,0.518896,0.515403,0.518934,0.520468,0.516965,0.518075,0.519554,0.517431,0.519238,0.520973,0.516731,0.519305,0.52119,0.519999,0.519384,0.521879,0.519335,0.521417,0.52277,0.519504,0.519589,0.522826,0.521259,0.519674,0.524588,0.522731,0.524096,0.52535,0.524069,0.525506,0.528222,0.526521,0.528564,0.528721,0.527622,0.529202,0.532479,0.525487,0.533329,0.534124,0.532737,0.533342,0.536065,0.540998,0.540322,0.55324,0.552472,0.561658,0.573364,0.583282,0.586308,0.578219,0.578872,0.543] }, "kmer_count": { "AAAAA":125838, "AAAAT":81150, "AAAAC":101252, "AAAAG":95958, "AAATA":49662, "AAATT":54228, "AAATC":69705, "AAATG":54469, "AAACA":79952, "AAACT":58632, "AAACC":108154, 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"CTTGT":64278, "CTTGC":61697, "CTTGG":77893, "CTCAA":66177, "CTCAT":50184, "CTCAC":42863, "CTCAG":52907, "CTCTA":36109, "CTCTT":79172, "CTCTC":62156, "CTCTG":57098, "CTCCA":89283, "CTCCT":124719, "CTCCC":83375, "CTCCG":66240, "CTCGA":55842, "CTCGT":45432, "CTCGC":53782, "CTCGG":52868, "CTGAA":69821, "CTGAT":49903, "CTGAC":40909, "CTGAG":49319, "CTGTA":23022, "CTGTT":66518, "CTGTC":42815, "CTGTG":42621, "CTGCA":62321, "CTGCT":89311, "CTGCC":79938, "CTGCG":52079, "CTGGA":80502, "CTGGT":68586, "CTGGC":74587, "CTGGG":70444, "CCAAA":107074, "CCAAT":55010, "CCAAC":100786, "CCAAG":83364, "CCATA":45105, "CCATT":55943, "CCATC":77990, "CCATG":56523, "CCACA":82473, "CCACT":43858, "CCACC":117110, "CCACG":53082, "CCAGA":80268, "CCAGT":53781, "CCAGC":112139, "CCAGG":70683, "CCTAA":32438, "CCTAT":37593, "CCTAC":42897, "CCTAG":29391, "CCTTA":32970, "CCTTT":82074, "CCTTC":107451, "CCTTG":74907, "CCTCA":76972, "CCTCT":76409, "CCTCC":132609, "CCTCG":69359, "CCTGA":63600, "CCTGT":56821, "CCTGC":98825, "CCTGG":72270, "CCCAA":99765, "CCCAT":59457, "CCCAC":85701, "CCCAG":80028, "CCCTA":38115, "CCCTT":63726, "CCCTC":77263, "CCCTG":66131, "CCCCA":92589, "CCCCT":66544, "CCCCC":64693, "CCCCG":66408, "CCCGA":53900, "CCCGT":37809, "CCCGC":75359, "CCCGG":56684, "CCGAA":61577, "CCGAT":47248, "CCGAC":50592, "CCGAG":53691, "CCGTA":27056, "CCGTT":43684, "CCGTC":52721, "CCGTG":39782, "CCGCA":61766, "CCGCT":58489, "CCGCC":114720, "CCGCG":50597, "CCGGA":53736, "CCGGT":46745, "CCGGC":73343, "CCGGG":53226, "CGAAA":67459, "CGAAT":38790, "CGAAC":50002, "CGAAG":68572, "CGATA":34165, "CGATT":47789, "CGATC":46883, "CGATG":60753, "CGACA":49201, "CGACT":40301, "CGACC":57275, "CGACG":53847, "CGAGA":54582, "CGAGT":29906, "CGAGC":47781, "CGAGG":61117, "CGTAA":25801, "CGTAT":22453, "CGTAC":19098, "CGTAG":28658, "CGTTA":19615, "CGTTT":48137, "CGTTC":44902, "CGTTG":46554, "CGTCA":44030, "CGTCT":46670, "CGTCC":45282, "CGTCG":50234, "CGTGA":35251, "CGTGT":24495, "CGTGC":32162, "CGTGG":45116, "CGCAA":47751, "CGCAT":34611, "CGCAC":38007, "CGCAG":50875, "CGCTA":24077, "CGCTT":56186, "CGCTC":47879, "CGCTG":57823, "CGCCA":80223, "CGCCT":81224, "CGCCC":75048, "CGCCG":86261, "CGCGA":40641, "CGCGT":28152, "CGCGC":48371, "CGCGG":48995, "CGGAA":56513, "CGGAT":42183, "CGGAC":32357, "CGGAG":57018, "CGGTA":26814, "CGGTT":50809, "CGGTC":46757, "CGGTG":52144, "CGGCA":62664, "CGGCT":66524, "CGGCC":66855, "CGGCG":83155, "CGGGA":46485, "CGGGT":41514, "CGGGC":51481, "CGGGG":55531, "GAAAA":120978, "GAAAT":70940, "GAAAC":74526, "GAAAG":81022, "GAATA":44830, "GAATT":51139, "GAATC":52762, "GAATG":46154, "GAACA":66721, "GAACT":52962, "GAACC":73337, "GAACG":45225, "GAAGA":113276, "GAAGT":52253, "GAAGC":91882, "GAAGG":88263, "GATAA":47935, "GATAT":38788, "GATAC":30196, "GATAG":39894, "GATTA":34278, "GATTT":67555, "GATTC":54262, "GATTG":53186, "GATCA":51634, "GATCT":47728, "GATCC":49264, "GATCG":42887, "GATGA":75906, "GATGT":45963, "GATGC":52263, "GATGG":67956, "GACAA":64916, "GACAT":42560, "GACAC":33487, "GACAG":41886, "GACTA":26086, "GACTT":48701, "GACTC":33053, "GACTG":37658, "GACCA":60882, "GACCT":49228, "GACCC":56596, "GACCG":46383, "GACGA":63136, "GACGT":30642, "GACGC":43082, "GACGG":49560, "GAGAA":100208, "GAGAT":58461, "GAGAC":56023, "GAGAG":51633, "GAGTA":23087, "GAGTT":45534, "GAGTC":29083, "GAGTG":25837, "GAGCA":58298, "GAGCT":44070, "GAGCC":54802, "GAGCG":41466, "GAGGA":105138, "GAGGT":54041, "GAGGC":71567, "GAGGG":55786, "GTAAA":27806, "GTAAT":22201, "GTAAC":24196, "GTAAG":19497, "GTATA":16005, "GTATT":30461, "GTATC":27914, "GTATG":25769, "GTACA":20525, "GTACT":19595, "GTACC":21677, "GTACG":19271, "GTAGA":36251, "GTAGT":23882, "GTAGC":29679, "GTAGG":32855, "GTTAA":24826, "GTTAT":30775, "GTTAC":26530, "GTTAG":19605, "GTTTA":29742, "GTTTT":83612, "GTTTC":69496, "GTTTG":70456, "GTTCA":53423, "GTTCT":63330, "GTTCC":64272, "GTTCG":46268, "GTTGA":64151, "GTTGT":57064, "GTTGC":54498, "GTTGG":73348, "GTCAA":51231, "GTCAT":40398, "GTCAC":32018, "GTCAG":36018, "GTCTA":27644, "GTCTT":61011, "GTCTC":55626, "GTCTG":46457, "GTCCA":45426, "GTCCT":46692, "GTCCC":40287, "GTCCG":30540, "GTCGA":45293, "GTCGT":40717, "GTCGC":45045, "GTCGG":43344, "GTGAA":51881, "GTGAT":42404, "GTGAC":30021, "GTGAG":34447, "GTGTA":17086, "GTGTT":40280, "GTGTC":25784, "GTGTG":29677, "GTGCA":30695, "GTGCT":41188, "GTGCC":40052, "GTGCG":31150, "GTGGA":54628, "GTGGT":55457, "GTGGC":55515, "GTGGG":58532, "GCAAA":69588, "GCAAT":44685, "GCAAC":61214, "GCAAG":56652, "GCATA":33939, "GCATT":43337, "GCATC":52259, "GCATG":39344, "GCACA":38865, "GCACT":29319, "GCACC":60909, "GCACG":35739, "GCAGA":64911, "GCAGT":36706, "GCAGC":68946, "GCAGG":83683, "GCTAA":24968, "GCTAT":37313, "GCTAC":32985, "GCTAG":21625, "GCTTA":30643, "GCTTT":75368, "GCTTC":96979, "GCTTG":71420, "GCTCA":50182, "GCTCT":48819, "GCTCC":67519, "GCTCG":47975, "GCTGA":65314, "GCTGT":48627, "GCTGC":68577, "GCTGG":96928, "GCCAA":84652, "GCCAT":59695, "GCCAC":66089, "GCCAG":72671, "GCCTA":40065, "GCCTT":72059, "GCCTC":81878, "GCCTG":75372, "GCCCA":75914, "GCCCT":62021, "GCCCC":65710, "GCCCG":54471, "GCCGA":70002, "GCCGT":53331, "GCCGC":82594, "GCCGG":67597, "GCGAA":56589, "GCGAT":47844, "GCGAC":47876, "GCGAG":45943, "GCGTA":21802, "GCGTT":40281, "GCGTC":39263, "GCGTG":34192, "GCGCA":40598, "GCGCT":43207, "GCGCC":62753, "GCGCG":46213, "GCGGA":57963, "GCGGT":52073, "GCGGC":76921, "GCGGG":61800, "GGAAA":87070, "GGAAT":55459, "GGAAC":62755, "GGAAG":81043, "GGATA":45655, "GGATT":58183, "GGATC":45103, "GGATG":62284, "GGACA":45451, "GGACT":33325, "GGACC":38425, "GGACG":42251, "GGAGA":98799, "GGAGT":36941, "GGAGC":59303, "GGAGG":103935, "GGTAA":25579, "GGTAT":29007, "GGTAC":21768, "GGTAG":37721, "GGTTA":30781, "GGTTT":84674, "GGTTC":65419, "GGTTG":80739, "GGTCA":46147, "GGTCT":58347, "GGTCC":36520, "GGTCG":46742, "GGTGA":62555, "GGTGT":40852, "GGTGC":48540, "GGTGG":81838, "GGCAA":71164, "GGCAT":55103, "GGCAC":42904, "GGCAG":67309, "GGCTA":38338, "GGCTT":84536, "GGCTC":55604, "GGCTG":87734, "GGCCA":59907, "GGCCT":56615, "GGCCC":48297, "GGCCG":61753, "GGCGA":78998, "GGCGT":49272, "GGCGC":59066, "GGCGG":96480, "GGGAA":58942, "GGGAT":49496, "GGGAC":36142, "GGGAG":64971, "GGGTA":27073, "GGGTT":72343, "GGGTC":45947, "GGGTG":56254, "GGGCA":56442, "GGGCT":59943, "GGGCC":44302, "GGGCG":60791, "GGGGA":59567, "GGGGT":56391, "GGGGC":53326, "GGGGG":44527 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/AM-AL-1B_S48_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/AM-AL-1B_S48_R2_001.fastq.gz -o AM-AL-1B.trim.R1.fastq.gz -O AM-AL-1B.trim.R2.fastq.gz -m --merged_out AM-AL-1B.trim.merged.fastq.gz " }