##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-1A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 591352 Sequences flagged as poor quality 0 Sequence length 31-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26378197757004 40.0 40.0 40.0 40.0 40.0 2 39.15258593866259 40.0 40.0 40.0 40.0 40.0 3 39.149958062203225 40.0 40.0 40.0 40.0 40.0 4 39.08344945142656 40.0 40.0 40.0 40.0 40.0 5 39.179189382973256 40.0 40.0 40.0 40.0 40.0 6 39.254001001095794 40.0 40.0 40.0 40.0 40.0 7 39.27393498288667 40.0 40.0 40.0 40.0 40.0 8 39.30510423571747 40.0 40.0 40.0 40.0 40.0 9 39.33003693231781 40.0 40.0 40.0 40.0 40.0 10-11 39.32269781788173 40.0 40.0 40.0 40.0 40.0 12-13 39.31729663550643 40.0 40.0 40.0 40.0 40.0 14-15 39.323428347244956 40.0 40.0 40.0 40.0 40.0 16-17 39.46813403860983 40.0 40.0 40.0 40.0 40.0 18-19 39.474302953232595 40.0 40.0 40.0 40.0 40.0 20-21 39.491984469486866 40.0 40.0 40.0 40.0 40.0 22-23 39.491439954544845 40.0 40.0 40.0 40.0 40.0 24-25 39.49147039326831 40.0 40.0 40.0 40.0 40.0 26-27 39.47739417470474 40.0 40.0 40.0 40.0 40.0 28-29 39.47810779366604 40.0 40.0 40.0 40.0 40.0 30-31 39.449360110391105 40.0 40.0 40.0 40.0 40.0 32-33 39.43418240353857 40.0 40.0 40.0 40.0 40.0 34-35 39.42675500822687 40.0 40.0 40.0 40.0 40.0 36-37 39.41566419817354 40.0 40.0 40.0 40.0 40.0 38-39 39.393299088784666 40.0 40.0 40.0 40.0 40.0 40-41 39.37383001387697 40.0 40.0 40.0 40.0 40.0 42-43 39.369200103142944 40.0 40.0 40.0 40.0 40.0 44-45 39.35147670315007 40.0 40.0 40.0 40.0 40.0 46-47 39.325017296198766 40.0 40.0 40.0 40.0 40.0 48-49 39.3181440342241 40.0 40.0 40.0 40.0 40.0 50-51 39.296090313513666 40.0 40.0 40.0 40.0 40.0 52-53 39.278935273543965 40.0 40.0 40.0 40.0 40.0 54-55 39.24813831278212 40.0 40.0 40.0 40.0 40.0 56-57 39.232377520161606 40.0 40.0 40.0 40.0 40.0 58-59 39.20571556698707 40.0 40.0 40.0 40.0 40.0 60-61 39.18060111343732 40.0 40.0 40.0 40.0 40.0 62-63 39.38977910521817 40.0 40.0 40.0 40.0 40.0 64-65 39.38147861299527 40.0 40.0 40.0 40.0 40.0 66-67 39.37592660610846 40.0 40.0 40.0 40.0 40.0 68-69 39.369984808118645 40.0 40.0 40.0 40.0 40.0 70-71 39.36452445386254 40.0 40.0 40.0 40.0 40.0 72-73 39.36910835389335 40.0 40.0 40.0 40.0 40.0 74-75 39.354970005772444 40.0 40.0 40.0 40.0 40.0 76-77 39.34572134300139 40.0 40.0 40.0 40.0 40.0 78-79 39.340982947857285 40.0 40.0 40.0 40.0 40.0 80-81 39.32714344657688 40.0 40.0 40.0 40.0 40.0 82-83 39.31750937248525 40.0 40.0 40.0 40.0 40.0 84-85 39.328971372187176 40.0 40.0 40.0 40.0 40.0 86-87 39.326571186792236 40.0 40.0 40.0 40.0 40.0 88-89 39.333923988828296 40.0 40.0 40.0 40.0 40.0 90-91 39.475363779073675 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2530038335244811 1101 2 0.1493559016219308 1101 3 0.32143583840237966 1101 4 0.2845627605828227 1101 5 0.001492922232408489 1101 6 0.23053599644153167 1101 7 0.25195533252647095 1101 8 -0.2416774477665129 1101 9 0.04838884511624286 1101 10-11 0.10679620284795988 1101 12-13 0.0811525880325874 1101 14-15 -0.05495853575972376 1101 16-17 0.014588181196572236 1101 18-19 0.006442896307014223 1101 20-21 0.058064398196393086 1101 22-23 -0.023630184943726817 1101 24-25 0.08336587051400812 1101 26-27 0.014533138803010104 1101 28-29 0.14270998486728104 1101 30-31 -0.023150891278042707 1101 32-33 -0.03812213516302876 1101 34-35 0.03446808915869326 1101 36-37 0.07767219734783026 1101 38-39 0.12077035839616457 1101 40-41 0.034136643425874524 1101 42-43 -0.043702101571852836 1101 44-45 0.11453601221856502 1101 46-47 0.05563444759491887 1101 48-49 -0.03363535939384832 1101 50-51 0.051721512891980126 1101 52-53 0.16820581766424425 1101 54-55 0.22557298163341244 1101 56-57 0.03484196456909672 1101 58-59 0.05894036969274197 1101 60-61 0.09735637856708479 1101 62-63 -0.006078401736957062 1101 64-65 0.1905515571656835 1101 66-67 -0.026488455779109188 1101 68-69 0.04693759508956674 1101 70-71 0.08685424847255518 1101 72-73 0.2403677975289824 1101 74-75 0.1441083806350889 1101 76-77 0.06944159809026473 1101 78-79 0.014101592892750148 1101 80-81 0.021159185081302212 1101 82-83 0.06002901589493348 1101 84-85 -0.04724041812309565 1101 86-87 0.2984047277212696 1101 88-89 0.12199201413151428 1101 90-91 0.08229310092423958 1102 1 0.3179057217140411 1102 2 0.1141096965127133 1102 3 0.19103968882814826 1102 4 -0.07478562890255347 1102 5 -0.2891771999445609 1102 6 -0.1409190202189663 1102 7 -0.24763741090188773 1102 8 -0.18899325672615674 1102 9 0.0014062460788508702 1102 10-11 -0.022896503557078063 1102 12-13 -0.08195366890928568 1102 14-15 -0.051256203290265034 1102 16-17 -0.057014928762697537 1102 18-19 -0.011809602767407057 1102 20-21 -0.11922179210349526 1102 22-23 0.03753234745168044 1102 24-25 -0.1306659695452339 1102 26-27 -0.040279893407280554 1102 28-29 -0.04053696070843671 1102 30-31 -0.05017791830506724 1102 32-33 -0.05570283007423171 1102 34-35 0.02677608889557348 1102 36-37 0.01156660121647235 1102 38-39 -0.05288346348982742 1102 40-41 -0.03769398952984204 1102 42-43 -0.08512338551678766 1102 44-45 -0.1403609433358639 1102 46-47 -0.12806897500387748 1102 48-49 -0.06879455678203072 1102 50-51 -0.06880203226301518 1102 52-53 -0.09537340215767642 1102 54-55 -0.005583871495346671 1102 56-57 0.0652947461897142 1102 58-59 0.03846768487918695 1102 60-61 -0.1428967930280649 1102 62-63 0.07334956206221221 1102 64-65 -0.17368372767401752 1102 66-67 -0.06097278758749525 1102 68-69 0.05013023529055971 1102 70-71 -0.12918129489348473 1102 72-73 -0.044325739774059514 1102 74-75 -0.08455728128341633 1102 76-77 -0.16570415142313522 1102 78-79 -0.039386126061508264 1102 80-81 0.01965122358873117 1102 82-83 0.27626386194468466 1102 84-85 0.2090174834818015 1102 86-87 -0.5526591020659595 1102 88-89 -0.0046975529971078345 1102 90-91 -0.07326245463131187 1103 1 0.1923613666873294 1103 2 0.2164745519264173 1103 3 0.16234250408781037 1103 4 -0.11227642032715579 1103 5 -0.06454391106106527 1103 6 0.08780054485652045 1103 7 -0.09465663001469693 1103 8 -0.036432107122941204 1103 9 -0.15510130262168786 1103 10-11 -0.0036484828179652595 1103 12-13 -0.1303343584101171 1103 14-15 0.022626878108873427 1103 16-17 0.031063812716453754 1103 18-19 -0.035665175004602645 1103 20-21 -0.003787753478768252 1103 22-23 -0.01538408997196683 1103 24-25 -0.001499730280798417 1103 26-27 0.01894779852740669 1103 28-29 -0.023489672925293803 1103 30-31 -0.12410576037449772 1103 32-33 -0.09056522593937899 1103 34-35 -0.011428772302984669 1103 36-37 -0.014155845222184382 1103 38-39 -0.004207439588427064 1103 40-41 -0.10725316330248091 1103 42-43 -0.07139699776597297 1103 44-45 -0.0013079887434699344 1103 46-47 -0.06655568718151272 1103 48-49 0.10657183962649697 1103 50-51 -0.02497403927942088 1103 52-53 0.016002369139371808 1103 54-55 -0.10264247610252397 1103 56-57 0.04198465205028867 1103 58-59 0.04572895424021084 1103 60-61 -0.0336711087832029 1103 62-63 -0.20878444935365081 1103 64-65 0.047448783926142823 1103 66-67 -0.10722370685027727 1103 68-69 -0.02121807070987103 1103 70-71 -0.13790059559872958 1103 72-73 0.08180613682132076 1103 74-75 -0.08437298681946004 1103 76-77 0.07882622715025178 1103 78-79 -0.1917675689049858 1103 80-81 -0.09672260535900534 1103 82-83 -0.14648864265123507 1103 84-85 -0.39523657082975205 1103 86-87 -0.02950380809464548 1103 88-89 0.0126008892605185 1103 90-91 0.5267375453686824 1104 1 0.2848547642928736 1104 2 0.06996080964226081 1104 3 0.1664711307989748 1104 4 0.13485716865801578 1104 5 -0.026678821906862993 1104 6 0.12970214499874544 1104 7 0.021278376815686784 1104 8 0.01954846014410805 1104 9 -0.10672580945713861 1104 10-11 0.07219868245882566 1104 12-13 0.020791618131696055 1104 14-15 0.019832087068785142 1104 16-17 0.04499815815594843 1104 18-19 -0.03893251970439593 1104 20-21 0.012811499831180129 1104 22-23 -0.001350259551486488 1104 24-25 0.0029540649330570545 1104 26-27 0.026033339951382573 1104 28-29 -0.07160263627889663 1104 30-31 -0.02933894672184323 1104 32-33 -0.01393051864240391 1104 34-35 -0.03901680238305971 1104 36-37 0.009689155797445892 1104 38-39 -0.01752449524220623 1104 40-41 0.027545179862364932 1104 42-43 -0.007734941100267179 1104 44-45 0.032747409365569524 1104 46-47 0.06059322899671571 1104 48-49 -0.04934639014755504 1104 50-51 0.03414883586577133 1104 52-53 0.05668405405289434 1104 54-55 0.11416905773515396 1104 56-57 -0.02193252715872518 1104 58-59 0.029214534719706364 1104 60-61 0.07905076233521413 1104 62-63 -0.021206971447469414 1104 64-65 0.1111343137898757 1104 66-67 0.016188568713310758 1104 68-69 0.12190167489988113 1104 70-71 0.1238425644206842 1104 72-73 0.0892601734574825 1104 74-75 -0.13341826904012777 1104 76-77 -0.14908642294827246 1104 78-79 0.020748264019189833 1104 80-81 0.12937676435306855 1104 82-83 0.12943530871356757 1104 84-85 0.318391765784952 1104 86-87 0.18416724052006828 1104 88-89 0.17506587525491568 1104 90-91 0.1631011817323227 1201 1 0.3203272860092241 1201 2 0.36297403588522315 1201 3 0.23906443474065497 1201 4 0.3002209994668661 1201 5 0.1468634540545537 1201 6 0.14030059975451792 1201 7 0.0306126995709306 1201 8 0.24527983209399196 1201 9 0.01747341355564913 1201 10-11 0.1246863510606886 1201 12-13 0.07629644156353521 1201 14-15 0.030900226228069982 1201 16-17 0.0061313372558657875 1201 18-19 -0.06628596158314082 1201 20-21 0.09608532234634737 1201 22-23 0.031504950191404646 1201 24-25 -0.03553561161328389 1201 26-27 0.09394900628339542 1201 28-29 0.06941443124914315 1201 30-31 0.05562853330783213 1201 32-33 0.023598864142236664 1201 34-35 0.06328442960092673 1201 36-37 0.025719183159154113 1201 38-39 0.016916797642643644 1201 40-41 -0.10090535879586326 1201 42-43 0.02278881560637558 1201 44-45 -0.12336843992682844 1201 46-47 -0.0608502957997743 1201 48-49 -0.0031812560906132603 1201 50-51 0.0713812240238596 1201 52-53 -0.12605096341481214 1201 54-55 0.13573889543688722 1201 56-57 0.058837454784502086 1201 58-59 -0.1101480408256279 1201 60-61 0.033259041836792846 1201 62-63 -0.32531450471833523 1201 64-65 -0.30400364936940605 1201 66-67 -0.25667568993214473 1201 68-69 -0.08971023418339286 1201 70-71 0.09936042568446624 1201 72-73 -0.3009895987449127 1201 74-75 0.024618322315951957 1201 76-77 -0.21641028179237054 1201 78-79 -0.12091888830212127 1201 80-81 -0.14329664445165236 1201 82-83 -0.6518783917290847 1201 84-85 -0.42686312918591796 1201 86-87 -2.04896699599999E-4 1201 88-89 0.5345751962246794 1201 90-91 -0.15411351846110932 1202 1 0.07475282589968657 1202 2 -0.010466477244996497 1202 3 -0.01955486178780319 1202 4 0.16296033181448166 1202 5 0.12577013682595606 1202 6 0.05727513066936751 1202 7 0.20318105792908625 1202 8 0.04872263598412019 1202 9 0.11517474011388629 1202 10-11 0.06493833465051324 1202 12-13 -0.07387244991692654 1202 14-15 0.02144646834056374 1202 16-17 0.003203329592231796 1202 18-19 0.01595731711904591 1202 20-21 -0.09655758983424079 1202 22-23 0.032486228435438136 1202 24-25 0.10097618580024204 1202 26-27 -0.019945077308520354 1202 28-29 0.003274778195148542 1202 30-31 0.024321758906040714 1202 32-33 0.09809958650556183 1202 34-35 0.046877396156631335 1202 36-37 0.051814466453905084 1202 38-39 -0.02650279009146317 1202 40-41 0.03249007858298825 1202 42-43 0.07234847391568167 1202 44-45 -0.022316353472255912 1202 46-47 0.01701218157226947 1202 48-49 0.0019271387362564951 1202 50-51 -0.06926059055386702 1202 52-53 0.07137622845147007 1202 54-55 0.023768887276297335 1202 56-57 0.02099341736681737 1202 58-59 0.09014610784762311 1202 60-61 -0.13238207602094576 1202 62-63 0.07201137150624248 1202 64-65 -0.08225603254595626 1202 66-67 -0.06528373691581635 1202 68-69 -0.22284084345411515 1202 70-71 -0.022258035716234303 1202 72-73 -0.18293007154963448 1202 74-75 -0.14803371225984563 1202 76-77 -0.1379727812604088 1202 78-79 -0.18861058689432753 1202 80-81 0.10708410101611321 1202 82-83 0.21105514795760172 1202 84-85 -0.11791025127374155 1202 86-87 -0.1024848448741622 1202 88-89 -0.0024758317958273324 1202 90-91 0.5267375453686824 1203 1 0.23712981997605453 1203 2 0.14274231128740666 1203 3 0.03415320693370205 1203 4 0.11854558061214959 1203 5 -0.06330908908122979 1203 6 -0.16539249018462954 1203 7 0.045223018817409866 1203 8 0.12322931946583537 1203 9 0.10705914332142896 1203 10-11 -0.037522534494030424 1203 12-13 -0.10666429862151006 1203 14-15 -0.0926706664951169 1203 16-17 -0.09662768583324066 1203 18-19 -0.0011763508471176465 1203 20-21 -0.01296360655253892 1203 22-23 0.03217678481672692 1203 24-25 -0.002645722474326817 1203 26-27 0.024928417802939862 1203 28-29 0.12438817468500218 1203 30-31 -0.00185750486837577 1203 32-33 -0.08713362242634304 1203 34-35 -0.06392573645338473 1203 36-37 -0.011908250224117012 1203 38-39 -0.05247957913972101 1203 40-41 -0.11104348713647028 1203 42-43 -0.0835882596060813 1203 44-45 -0.037010037438513166 1203 46-47 -0.00977890833623718 1203 48-49 -0.08020243715633768 1203 50-51 -0.09005997811490118 1203 52-53 -0.18573825856201154 1203 54-55 0.0017336110922627768 1203 56-57 -0.0023748185201242222 1203 58-59 -0.023051784622339255 1203 60-61 -0.16547956506477846 1203 62-63 -0.026643825552298495 1203 64-65 -0.10881648209358019 1203 66-67 -0.03652153905852629 1203 68-69 -0.02230682745374679 1203 70-71 0.006324893340618587 1203 72-73 -0.1367825307181505 1203 74-75 0.005122487975562251 1203 76-77 -0.035446227416343845 1203 78-79 0.019511702613066007 1203 80-81 -0.013000755519939844 1203 82-83 -0.029490492185821893 1203 84-85 0.15521523080315092 1203 86-87 0.16334158761893747 1203 88-89 0.3697178101029266 1203 90-91 0.3267375453686796 1204 1 0.1808050451407226 1204 2 0.023356491807490443 1204 3 -0.13588159463759553 1204 4 -0.10805297654158608 1204 5 -0.14811738171315625 1204 6 -0.1703961495773214 1204 7 -0.2437345770362711 1204 8 -0.2304621862622369 1204 9 -0.06947294731202192 1204 10-11 -0.1567216502652684 1204 12-13 -0.09179521526204582 1204 14-15 -0.13319283942755078 1204 16-17 -0.15716449633644913 1204 18-19 -0.07127239123486362 1204 20-21 -0.16028776899970154 1204 22-23 -0.1448855443493784 1204 24-25 -0.11787178332480153 1204 26-27 0.014921099017563222 1204 28-29 -0.07114288534397417 1204 30-31 -0.09115415465702625 1204 32-33 -0.18171906280468164 1204 34-35 0.002474255566511374 1204 36-37 -0.21825796385103047 1204 38-39 -0.16317801838557244 1204 40-41 -0.058529281635074426 1204 42-43 -0.15521035294408136 1204 44-45 -0.1664408854326922 1204 46-47 -0.19521688058052433 1204 48-49 -0.24415854850297336 1204 50-51 -0.04352395530423081 1204 52-53 -0.2199553569241317 1204 54-55 -0.35828876642759155 1204 56-57 -0.16292983808969552 1204 58-59 -0.09328718965104343 1204 60-61 -0.34355847114633775 1204 62-63 -0.18530678295807945 1204 64-65 -0.22599715586197533 1204 66-67 -0.09956979484725537 1204 68-69 0.04247682322311164 1204 70-71 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0.09774959632249391 2401 48-49 0.1598153156674087 2401 50-51 0.1146598087418127 2401 52-53 0.1240495682796734 2401 54-55 0.10899349816315862 2401 56-57 0.14128970903765037 2401 58-59 0.15974079013684417 2401 60-61 0.12147418345779215 2401 62-63 0.23478619735762152 2401 64-65 0.19846747203039428 2401 66-67 0.06805399239710397 2401 68-69 0.11484902142916553 2401 70-71 -0.002614278037142981 2401 72-73 0.13335915471814275 2401 74-75 0.2739841863933279 2401 76-77 0.12253107929534224 2401 78-79 -0.007482418900657706 2401 80-81 0.15373532818698266 2401 82-83 0.28808349393035826 2401 84-85 -0.06739901018416106 2401 86-87 0.06576195056562284 2401 88-89 0.2357246215120412 2401 90-91 0.386386668175696 2402 1 0.30095974836101647 2402 2 0.23182823274638054 2402 3 0.23958453909280308 2402 4 0.21524080705034265 2402 5 0.1854097016562477 2402 6 0.20931104877242035 2402 7 0.17280326646811517 2402 8 0.18691661484552213 2402 9 0.1680629479739153 2402 10-11 0.17971250811342543 2402 12-13 0.09141811671916145 2402 14-15 0.11764667535639717 2402 16-17 0.0461081478898393 2402 18-19 0.04336826840567909 2402 20-21 0.007872421881181424 2402 22-23 0.03568124458593047 2402 24-25 -0.007500220223711551 2402 26-27 -0.0027291170343346494 2402 28-29 0.024229951560542418 2402 30-31 0.06116140561449157 2402 32-33 0.022890342429739974 2402 34-35 0.03648109201542127 2402 36-37 0.04217669924835121 2402 38-39 0.06471660381667022 2402 40-41 0.08338793361293284 2402 42-43 0.07995113891580274 2402 44-45 0.10852329641226532 2402 46-47 0.1122834302068938 2402 48-49 0.04791311654852137 2402 50-51 0.24645001221260543 2402 52-53 0.15321597851909274 2402 54-55 0.04539901722952777 2402 56-57 0.1444425399442224 2402 58-59 0.21383895666023278 2402 60-61 0.21335752769113725 2402 62-63 0.07649152839336182 2402 64-65 0.09010622311990346 2402 66-67 0.10426117198134222 2402 68-69 0.09270846000226385 2402 70-71 0.13191784985177435 2402 72-73 0.002235009086007267 2402 74-75 0.10856184017503523 2402 76-77 -0.18517219681104535 2402 78-79 -0.027827739076528246 2402 80-81 -0.13106201567413223 2402 82-83 -0.035923201124077764 2402 84-85 -0.11303873745082171 2402 86-87 -0.17435692808123093 2402 88-89 -0.23320020810854913 2402 90-91 -0.055557536598534796 2403 1 -0.3219976096102357 2403 2 -0.20116535863876095 2403 3 -0.15750940329382246 2403 4 -0.08770365363147903 2403 5 0.07713973521369155 2403 6 -0.023858637406327432 2403 7 0.06780441527003944 2403 8 0.037451360666011624 2403 9 0.10742229120650393 2403 10-11 0.08401776443517406 2403 12-13 0.1283194259512399 2403 14-15 0.09296854552916045 2403 16-17 -0.00430533855127635 2403 18-19 0.05190394898719575 2403 20-21 0.10525317176278293 2403 22-23 0.051872314565606814 2403 24-25 0.04293154468882676 2403 26-27 0.03745646794163804 2403 28-29 0.052415354482945986 2403 30-31 0.10898483265605563 2403 32-33 0.06656579184349454 2403 34-35 0.04911491168724069 2403 36-37 0.07575704706535902 2403 38-39 0.0291604754861865 2403 40-41 -0.024208985084975154 2403 42-43 0.125831444217269 2403 44-45 0.08514407118411071 2403 46-47 -0.07560452639901172 2403 48-49 0.23158766337490277 2403 50-51 0.10506759610348126 2403 52-53 0.08897953089343247 2403 54-55 0.06515576244349575 2403 56-57 0.020701766521312948 2403 58-59 0.10457383494181016 2403 60-61 0.025282304741679695 2403 62-63 0.02979900083030884 2403 64-65 0.19695215867378835 2403 66-67 0.09928104174374397 2403 68-69 -0.006188355786129307 2403 70-71 0.16808142386537384 2403 72-73 0.22609840749700538 2403 74-75 0.11622679985827489 2403 76-77 0.12636455306068228 2403 78-79 0.20502809782323794 2403 80-81 0.22078119085332304 2403 82-83 0.302733432064592 2403 84-85 0.3497173953053121 2403 86-87 0.4652423777832908 2403 88-89 0.3704402314490025 2403 90-91 0.3934042120353496 2404 1 -0.49318509080674744 2404 2 -0.27183388637654815 2404 3 -0.39526518302808 2404 4 -0.29817310694213717 2404 5 -0.3282886275320962 2404 6 -0.17186730161579078 2404 7 -0.12651747347083386 2404 8 0.04428968681587264 2404 9 0.016848868838302167 2404 10-11 0.06337101370483111 2404 12-13 0.03856976234366272 2404 14-15 0.06803919567430228 2404 16-17 0.06611456910984259 2404 18-19 0.03354938072069302 2404 20-21 0.030420712755521606 2404 22-23 0.019813429100651092 2404 24-25 0.04923258938455888 2404 26-27 -0.006484491524375358 2404 28-29 0.10303483890751863 2404 30-31 0.11352041999915485 2404 32-33 0.07839796600982396 2404 34-35 0.01930892461133027 2404 36-37 -0.0473077412523466 2404 38-39 0.016366312760695223 2404 40-41 -0.03126460645882645 2404 42-43 0.028847824864783433 2404 44-45 0.14050928503674953 2404 46-47 0.11803671663572146 2404 48-49 0.10139682443161036 2404 50-51 0.10335107481082417 2404 52-53 0.1191754303870809 2404 54-55 0.14018559138406772 2404 56-57 0.07022946657328077 2404 58-59 0.11852911343557793 2404 60-61 0.1441615153979896 2404 62-63 0.03850126883070715 2404 64-65 0.014999296426687181 2404 66-67 0.044108833776398626 2404 68-69 0.1241670320007131 2404 70-71 0.06258541252476846 2404 72-73 0.0776588417024513 2404 74-75 0.07831691494323678 2404 76-77 0.19562691189496917 2404 78-79 0.19701098822726948 2404 80-81 0.12698375319102695 2404 82-83 0.299017659249202 2404 84-85 0.11880784733693872 2404 86-87 0.13672067340678495 2404 88-89 0.2575206753567727 2404 90-91 0.10067238246141841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 353.0 32 2444.0 33 4514.0 34 5704.0 35 7167.0 36 10453.0 37 16500.0 38 47606.0 39 288195.0 40 208405.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.20011566988761 14.605014391092741 26.05109091585523 31.14377902316442 2 26.46788230558741 19.333529513237067 26.90810324474796 27.29048493642756 3 26.13263751371882 20.092636904933027 21.482359586093303 32.292365995254855 4 26.292631123256538 21.94817976433664 20.48170970927637 31.277479403130453 5 26.911551833763987 21.717183674021566 20.585032265046877 30.786232227167577 6 26.912397353860307 24.354191750429525 23.43240574141966 25.301005154290507 7 25.208843463791446 24.193712036147673 23.453543743827705 27.143900756233176 8 24.147208430849982 25.501393417118738 24.03932006655934 26.312078085471935 9 24.006006574764267 25.66812998011337 24.55525642933481 25.77060701578755 10-11 23.962124081765175 24.671853650617567 24.39190194672547 26.974120320891785 12-13 24.269470636778095 24.489474965840987 24.034331497991044 27.206722899389874 14-15 24.734168481716473 24.009219551130293 23.92255374125732 27.334058225895912 16-17 24.66103099338465 23.878163936200437 23.726562182929964 27.73424288748495 18-19 24.780672087014164 24.066292157632002 23.731719855517525 27.42131589983631 20-21 24.87232646545543 24.230153952299137 23.515266710859184 27.38225287138625 22-23 24.763254373029937 23.860069806139155 23.639558165018464 27.737117655812444 24-25 24.825315548099947 24.135455701511113 23.6322866921901 27.40694205819884 26-27 24.934979504592867 24.088698440184526 23.553230563184023 27.42309149203858 28-29 24.8722419134458 23.825826242238126 23.746516457203153 27.55541538711292 30-31 25.141286408095347 24.067222229737954 23.653255590578876 27.13823577158782 32-33 25.08536106918487 24.171188133569302 23.54971843267997 27.193732364565864 34-35 25.07777582555138 23.857663464723995 23.735634746016803 27.328925963707817 36-37 25.138591452452747 24.1299536596022 23.727787765275497 27.00366712266955 38-39 25.30089435428504 24.086549345937858 23.58286535725395 27.02969094252315 40-41 25.193382128784663 23.94187092283044 23.644520673487392 27.220226274897502 42-43 25.31178892834366 24.169547039973548 23.68838035099875 26.830283680684047 44-45 25.444573469567477 24.078955183225915 23.564697907130583 26.91177344007602 46-47 25.343607609894015 23.89207732227202 23.55116981107864 27.21314525675533 48-49 25.491271026444778 24.155803096958202 23.519796585543045 26.833129291053982 50-51 25.604349072260042 24.10095150699506 23.4588287747416 26.835870646003308 52-53 25.58722907669137 23.91992240098146 23.53803648550255 26.954812036824627 54-55 25.618396536026594 24.19289525503398 23.469822566643376 26.718885642296048 56-57 25.779631935054994 24.138383170467957 23.25283351920336 26.829151375273696 58-59 25.785496666767216 23.996561189707702 23.31392718168381 26.90401496184127 60-61 25.874486514363003 24.153812970407984 23.26655519859103 26.70514531663799 62-63 26.13404863339119 24.054243587720684 22.965865460474415 26.845842318413716 64-65 25.990773567166258 23.921977713911925 23.13073195477179 26.956516764150024 66-67 26.239031770045386 23.997704627262767 22.951640669831498 26.811622932860345 68-69 26.502490926403823 23.98327422270676 22.74977114757416 26.764463703315254 70-71 26.50816308780116 23.837955928023614 22.663248965224376 26.99063201895085 72-73 26.43854644388449 23.969268917065108 22.651115537162568 26.941069101887834 74-75 26.724814852223894 23.936438841771555 22.542250836545385 26.79649546945917 76-77 26.734699241204012 23.72091626211314 22.438735014624 27.105649482058848 78-79 27.010079570924688 23.981804811244732 22.02842007459499 26.979695543235593 80-81 27.570485966200547 23.744670959834753 21.590867068512836 27.093976005451864 82-83 27.830007111308287 23.210918794078694 21.197570411738226 27.761503682874793 84-85 28.888191966366893 22.7828681375499 20.56721958992702 27.761720306156178 86-87 29.57669635761982 22.083190018146666 19.54502864326972 28.795084980963793 88-89 29.38326243299441 20.93817363270445 19.749718624215486 29.92884531008565 90-91 27.746034648260732 22.86419527357499 20.36842345842666 29.021346619737624 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 0.0 9 1.0 10 2.0 11 1.5 12 0.5 13 1.5 14 5.0 15 8.0 16 11.5 17 17.5 18 26.5 19 45.5 20 73.0 21 97.5 22 136.5 23 196.5 24 274.0 25 387.0 26 561.0 27 810.5 28 1155.5 29 1552.5 30 2029.0 31 2658.0 32 3464.5 33 4378.0 34 5587.0 35 7133.5 36 8946.5 37 10832.0 38 12898.0 39 15562.5 40 18353.0 41 21036.0 42 23889.5 43 26858.0 44 29832.5 45 32825.0 46 35398.5 47 37593.0 48 39418.0 49 40825.0 50 41850.0 51 42229.0 52 42041.5 53 41616.5 54 40932.5 55 39637.0 56 38036.0 57 36447.0 58 34771.5 59 32765.0 60 30539.5 61 28597.5 62 27012.5 63 25036.5 64 22696.5 65 20631.0 66 18492.5 67 16509.0 68 14538.0 69 12426.5 70 10568.0 71 8788.0 72 7093.0 73 5618.0 74 4331.0 75 3260.0 76 2476.0 77 1788.0 78 1264.0 79 892.0 80 606.0 81 412.0 82 267.5 83 175.5 84 119.0 85 76.5 86 44.0 87 30.0 88 22.5 89 13.0 90 7.0 91 3.5 92 1.0 93 0.5 94 1.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002367456269700618 2 0.009808033117331133 3 0.0018601442119076288 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 3.754542997026402E-4 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4918.0 32-33 11313.0 34-35 12969.0 36-37 14851.0 38-39 16512.0 40-41 17873.0 42-43 19533.0 44-45 20549.0 46-47 21406.0 48-49 21887.0 50-51 22777.0 52-53 23112.0 54-55 23133.0 56-57 23226.0 58-59 23114.0 60-61 23224.0 62-63 23121.0 64-65 22535.0 66-67 22395.0 68-69 22032.0 70-71 21329.0 72-73 20911.0 74-75 20544.0 76-77 19637.0 78-79 19213.0 80-81 18145.0 82-83 17794.0 84-85 17094.0 86-87 16110.0 88-89 15481.0 90-91 14614.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.65792829674966 #Duplication Level Percentage of deduplicated Percentage of total 1 99.67368092082424 99.33272546280605 2 0.31168498523671745 0.6212375981978853 3 0.013128019429185582 0.03924933656856339 4 9.723333973539044E-4 0.0038760292797612155 5 2.808796733170949E-4 0.0013995943171724757 6 2.5286143919312935E-4 0.0015119788305673083 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 8.455200963216493E-4 0.0 0.0 0.0 0.0 2 8.455200963216493E-4 0.0 0.0 0.0 0.0 3 8.455200963216493E-4 0.0 0.0 0.0 0.0 4 8.455200963216493E-4 0.0 0.0 0.0 0.0 5 8.455200963216493E-4 0.0 0.0 0.0 0.0 6 0.0010146241155859792 0.0 0.0 0.0 0.0 7 0.001521936173378969 0.0 0.0 0.0 0.0 8 0.005918640674251545 0.0 0.0 0.0 0.0 9 0.006256848712780205 0.0 0.0 0.0 0.0 10-11 0.006256848712780205 0.0 0.0 8.455200963216494E-5 0.0 12-13 0.006256848712780205 0.0 0.0 1.6910401926432988E-4 0.0 14-15 0.006256848712780205 0.0 0.0 1.6910401926432988E-4 0.0 16-17 0.006256848712780205 0.0 0.0 1.6910401926432988E-4 0.0 18-19 0.006256848712780205 0.0 0.0 1.6910401926432988E-4 8.455200963216494E-5 20-21 0.00634140072241237 0.0 0.0 1.6910401926432988E-4 1.6910401926432988E-4 22-23 0.006425952732044536 0.0 0.0 1.6910401926432988E-4 1.6910401926432988E-4 24-25 0.0065105047416767 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 26-27 0.006595056751308865 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 28-29 0.00667960876094103 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 30-31 0.0071023688091018545 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 32-33 0.007440576847630514 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 34-35 0.007440576847630514 0.0 0.0 1.6910401926432988E-4 3.3820803852865975E-4 36-37 0.007440576847630514 0.0 0.0 1.6910401926432988E-4 5.918640674251545E-4 38-39 0.007863336895791339 0.0 0.0 1.6910401926432988E-4 6.764160770573195E-4 40-41 0.008116992924687833 0.0 0.0 1.6910401926432988E-4 6.764160770573195E-4 42-43 0.008286096943952164 0.0 0.0 1.6910401926432988E-4 7.609680866894845E-4 44-45 0.008455200963216494 0.0 0.0 1.6910401926432988E-4 0.0010146241155859792 46-47 0.008455200963216494 0.0 0.0 1.6910401926432988E-4 0.0010146241155859792 48-49 0.008455200963216494 0.0 0.0 1.6910401926432988E-4 0.001268280144482474 50-51 0.008539752972848659 0.0 0.0 3.3820803852865975E-4 0.001437384163746804 52-53 0.008624304982480823 0.0 0.0 3.3820803852865975E-4 0.001521936173378969 54-55 0.008793409001745153 0.0 0.0 3.3820803852865975E-4 0.001521936173378969 56-57 0.009047065030641649 0.0 0.0 3.3820803852865975E-4 0.001521936173378969 58-59 0.009216169049905978 0.0 0.0 3.3820803852865975E-4 0.001521936173378969 60-61 0.009300721059538143 0.0 0.0 3.3820803852865975E-4 0.001521936173378969 62-63 0.009300721059538143 0.0 1.6910401926432988E-4 3.3820803852865975E-4 0.0016910401926432987 64-65 0.009723481107698967 0.0 1.6910401926432988E-4 3.3820803852865975E-4 0.0016910401926432987 66-67 0.009892585126963298 0.0 1.6910401926432988E-4 3.3820803852865975E-4 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