##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-19C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 717030 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25596418559893 40.0 40.0 40.0 40.0 40.0 2 39.181746928301465 40.0 40.0 40.0 40.0 40.0 3 39.150961605511625 40.0 40.0 40.0 40.0 40.0 4 39.04752102422493 40.0 40.0 40.0 40.0 40.0 5 39.209634185459464 40.0 40.0 40.0 40.0 40.0 6 39.24783621326862 40.0 40.0 40.0 40.0 40.0 7 39.29517035549419 40.0 40.0 40.0 40.0 40.0 8 39.2907856017182 40.0 40.0 40.0 40.0 40.0 9 39.32899878666164 40.0 40.0 40.0 40.0 40.0 10-11 39.3204384753776 40.0 40.0 40.0 40.0 40.0 12-13 39.321983738476774 40.0 40.0 40.0 40.0 40.0 14-15 39.32342858736733 40.0 40.0 40.0 40.0 40.0 16-17 39.46535570338759 40.0 40.0 40.0 40.0 40.0 18-19 39.47919054990726 40.0 40.0 40.0 40.0 40.0 20-21 39.49196825795295 40.0 40.0 40.0 40.0 40.0 22-23 39.49142992622345 40.0 40.0 40.0 40.0 40.0 24-25 39.49601550841666 40.0 40.0 40.0 40.0 40.0 26-27 39.48455992078435 40.0 40.0 40.0 40.0 40.0 28-29 39.46053861065785 40.0 40.0 40.0 40.0 40.0 30-31 39.36558814707678 40.0 40.0 40.0 40.0 40.0 32-33 39.33704372237448 40.0 40.0 40.0 40.0 40.0 34-35 39.31251665159128 40.0 40.0 40.0 40.0 40.0 36-37 39.2966817069337 40.0 40.0 40.0 40.0 40.0 38-39 39.255484353250914 40.0 40.0 40.0 40.0 40.0 40-41 39.220578855629626 40.0 40.0 40.0 40.0 40.0 42-43 39.19694673638914 40.0 40.0 40.0 40.0 40.0 44-45 39.189630260739726 40.0 40.0 40.0 40.0 40.0 46-47 39.14251420469473 40.0 40.0 40.0 40.0 40.0 48-49 39.11180549497598 40.0 40.0 40.0 40.0 40.0 50-51 39.07792540375909 40.0 40.0 40.0 40.0 40.0 52-53 39.03784014386498 40.0 40.0 40.0 40.0 40.0 54-55 39.00811452979762 40.0 40.0 40.0 40.0 40.0 56-57 38.97598470551405 40.0 40.0 40.0 40.0 40.0 58-59 38.950882299273545 40.0 40.0 40.0 40.0 40.0 60-61 38.90855138489202 40.0 40.0 40.0 40.0 40.0 62-63 39.35942587369411 40.0 40.0 40.0 40.0 40.0 64-65 39.34972719812457 40.0 40.0 40.0 40.0 40.0 66-67 39.34810663697188 40.0 40.0 40.0 40.0 40.0 68-69 39.342963344127725 40.0 40.0 40.0 40.0 40.0 70-71 39.349482418038235 40.0 40.0 40.0 40.0 40.0 72-73 39.35021252687449 40.0 40.0 40.0 40.0 40.0 74-75 39.349149099971875 40.0 40.0 40.0 40.0 40.0 76-77 39.319919612016136 40.0 40.0 40.0 40.0 40.0 78-79 39.32717355398492 40.0 40.0 40.0 40.0 40.0 80-81 39.32222600779356 40.0 40.0 40.0 40.0 40.0 82-83 39.301401042711845 40.0 40.0 40.0 40.0 40.0 84-85 39.338313962377114 40.0 40.0 40.0 40.0 40.0 86-87 39.35449871140444 40.0 40.0 40.0 40.0 40.0 88-89 39.345403723191794 40.0 40.0 40.0 40.0 40.0 90-91 39.473647476814676 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1671323521192818 1101 2 0.16737935775837798 1101 3 0.19802758391199404 1101 4 0.21498236393239267 1101 5 0.13236832098934315 1101 6 0.03290953526180829 1101 7 0.1498392811184388 1101 8 0.07175631785649728 1101 9 0.11514965979355196 1101 10-11 0.08269277101217654 1101 12-13 0.1318825331975404 1101 14-15 0.19083257080315974 1101 16-17 0.09182578305869527 1101 18-19 0.009675568608642493 1101 20-21 0.19220350060347613 1101 22-23 0.15139715013023647 1101 24-25 0.12282895028979368 1101 26-27 0.18868517923720418 1101 28-29 0.10232577433544066 1101 30-31 5.156098955794164E-4 1101 32-33 0.15673334450052323 1101 34-35 0.16024000802493532 1101 36-37 0.034972365952882 1101 38-39 0.12386711646845328 1101 40-41 0.09122448310460385 1101 42-43 0.1591562200535961 1101 44-45 0.05383967700578296 1101 46-47 -0.04179620336601175 1101 48-49 0.18866967965350057 1101 50-51 0.022551852643857728 1101 52-53 0.12388196763314596 1101 54-55 0.08957679658917073 1101 56-57 0.07677920474044697 1101 58-59 0.041293912093259166 1101 60-61 0.16755266884366193 1101 62-63 0.2059222516294028 1101 64-65 0.1498875106025963 1101 66-67 0.18391595002130146 1101 68-69 0.006255466407033339 1101 70-71 0.23795420880676943 1101 72-73 0.2251970791365423 1101 74-75 0.2511557685006096 1101 76-77 0.19005295472149442 1101 78-79 0.35253599595844065 1101 80-81 0.0751981499270542 1101 82-83 0.10590537896201369 1101 84-85 -0.07187237575895011 1101 86-87 -0.26431243736696075 1101 88-89 -0.12141986782752667 1101 90-91 0.4227584101078463 1102 1 0.2553981920087409 1102 2 0.14508154254002648 1102 3 0.1376476782591709 1102 4 0.07122685812245066 1102 5 -0.015752426110132944 1102 6 0.14900664853308143 1102 7 -0.03376017430690581 1102 8 -0.11031771836457693 1102 9 0.022559256459999233 1102 10-11 -0.026361990266067892 1102 12-13 -0.026558946001465245 1102 14-15 -0.04701968980454296 1102 16-17 0.12636890093927633 1102 18-19 0.11942439562483287 1102 20-21 0.029842965232184326 1102 22-23 0.06923171587516919 1102 24-25 0.040057161901032146 1102 26-27 -0.03243069033436541 1102 28-29 -0.057517819348817056 1102 30-31 -0.03652919548 1102 32-33 -0.016372680836695963 1102 34-35 -0.007589296244098875 1102 36-37 0.010513937942533857 1102 38-39 0.15738068983835518 1102 40-41 0.09320876440865788 1102 42-43 -0.016887519413131713 1102 44-45 0.041802197617919035 1102 46-47 -0.01229824902028298 1102 48-49 0.005478200012106527 1102 50-51 -0.09591353266147706 1102 52-53 0.15656534485295737 1102 54-55 -0.15344558280109766 1102 56-57 0.023349264187004337 1102 58-59 -0.2351294047743835 1102 60-61 -0.1601378751770497 1102 62-63 -0.10789263418814699 1102 64-65 0.05385996255302672 1102 66-67 -0.024344825436500628 1102 68-69 -0.08668431344792538 1102 70-71 -0.022225769787027616 1102 72-73 -0.4849537549225147 1102 74-75 -0.057869819520036 1102 76-77 -0.3155570246955932 1102 78-79 -0.27649149820022245 1102 80-81 -0.08353510244540274 1102 82-83 0.10231114691432452 1102 84-85 0.2124233437042662 1102 86-87 -0.15375761376866848 1102 88-89 0.6593287952740781 1102 90-91 0.4227584101078463 1103 1 0.20488396873321335 1103 2 0.10319430989552814 1103 3 0.08060691692816846 1103 4 0.17726095263913066 1103 5 0.020940893047914244 1103 6 0.05774586018043948 1103 7 -0.06826075386938868 1103 8 0.052314159787023584 1103 9 -0.03951668468302927 1103 10-11 0.0182975444596849 1103 12-13 0.010219277215000488 1103 14-15 -0.053110633125548645 1103 16-17 0.05653715863417119 1103 18-19 0.03191046028480571 1103 20-21 0.06883238544821069 1103 22-23 0.027314951384262542 1103 24-25 -0.008249555855734059 1103 26-27 0.07130730312432831 1103 28-29 0.010161789649536956 1103 30-31 0.00991701595326333 1103 32-33 -0.0737820245475973 1103 34-35 -0.11380121161492696 1103 36-37 0.015688997283703543 1103 38-39 -0.026896208485723605 1103 40-41 -0.12297689491316532 1103 42-43 -0.0346155845539613 1103 44-45 -0.04913770670984974 1103 46-47 0.07959736009541984 1103 48-49 0.03225267836524637 1103 50-51 -0.11872852747149665 1103 52-53 -0.008710474354373332 1103 54-55 -0.020545778898288347 1103 56-57 0.02714963139337101 1103 58-59 -0.2165228144805269 1103 60-61 -0.0937660487318368 1103 62-63 -0.2123314933834024 1103 64-65 -0.0873686738868642 1103 66-67 -0.23400827420017833 1103 68-69 0.03282881634558521 1103 70-71 -0.052827284663813145 1103 72-73 0.19968720590944855 1103 74-75 0.08848331494814943 1103 76-77 0.0759981206666609 1103 78-79 -0.16966509060731028 1103 80-81 0.06942666757537808 1103 82-83 -0.6112810211728217 1103 84-85 -0.26206278490882795 1103 86-87 -0.4364079070732103 1103 88-89 -0.12554118108525358 1103 90-91 0.4227584101078463 1104 1 0.2571384100798184 1104 2 0.2648302985560207 1104 3 0.2206887093383756 1104 4 0.1976645773491441 1104 5 0.2101496322415528 1104 6 0.03572932846891064 1104 7 0.17457655788984994 1104 8 0.16066686439094724 1104 9 0.164060738364455 1104 10-11 0.055319495445104394 1104 12-13 0.12116925368855647 1104 14-15 0.02522655105251914 1104 16-17 -0.018830544366210233 1104 18-19 -0.047092721051356534 1104 20-21 0.02995172886162578 1104 22-23 0.0846761512559766 1104 24-25 0.09481964932523823 1104 26-27 -0.028732726933327513 1104 28-29 0.026447702580647103 1104 30-31 0.09186626907666096 1104 32-33 0.03035310985190165 1104 34-35 -0.028443143378432012 1104 36-37 -0.019747798275872697 1104 38-39 0.0045697733181597755 1104 40-41 -0.032752068959375435 1104 42-43 0.028753511023936085 1104 44-45 0.04465795987619714 1104 46-47 0.08772117363908905 1104 48-49 0.0162012821562314 1104 50-51 0.009478804603418212 1104 52-53 -0.15210573941091354 1104 54-55 -0.04287183397917005 1104 56-57 -0.08205465796491751 1104 58-59 0.08358300103355276 1104 60-61 -0.12292634764022381 1104 62-63 0.05128798365981879 1104 64-65 -0.05273317158086144 1104 66-67 0.1509377501941529 1104 68-69 0.004424809552958209 1104 70-71 -0.07712330830374015 1104 72-73 -0.17340928969719727 1104 74-75 -0.15031612101510916 1104 76-77 0.07525296418379668 1104 78-79 0.12014386970700741 1104 80-81 0.022796619970598897 1104 82-83 0.21598497161041763 1104 84-85 0.05249159335108544 1104 86-87 -0.21144391285664454 1104 88-89 -0.20647207052678596 1104 90-91 0.4227584101078463 1201 1 0.24473337256384298 1201 2 0.3383579439211175 1201 3 0.11075472692552779 1201 4 0.03766659070824119 1201 5 0.12464364421417429 1201 6 0.15734079363379294 1201 7 0.11993444962832456 1201 8 0.09300655925265033 1201 9 -0.05813402896101394 1201 10-11 0.1651573857193256 1201 12-13 -0.02108943778817718 1201 14-15 0.11652437834062113 1201 16-17 -0.08243210060128092 1201 18-19 0.011953610230868605 1201 20-21 -0.022568344671739737 1201 22-23 -0.03712063446198499 1201 24-25 -0.07524627674559525 1201 26-27 0.0869027223717751 1201 28-29 -0.014663717398050835 1201 30-31 0.05221682235001879 1201 32-33 -0.027949294539567404 1201 34-35 0.022720864359740744 1201 36-37 -0.075938757224705 1201 38-39 0.062394783413033394 1201 40-41 -0.08031161300561251 1201 42-43 -0.14551976230150387 1201 44-45 -0.04486567402995689 1201 46-47 -0.024308008061190378 1201 48-49 0.057132040806394 1201 50-51 0.09114502523903667 1201 52-53 -0.04041641923673467 1201 54-55 0.038826728348489326 1201 56-57 -0.20126684767345182 1201 58-59 -0.029461341548717712 1201 60-61 -0.2266378880637916 1201 62-63 -0.005068938444310334 1201 64-65 -0.06678690350110372 1201 66-67 -0.24333026074861408 1201 68-69 0.11302449174302609 1201 70-71 0.029124530485489686 1201 72-73 -0.06342694812526162 1201 74-75 0.0010924446309132918 1201 76-77 0.36367969601214867 1201 78-79 0.39122247039302493 1201 80-81 0.10078303075658113 1201 82-83 0.008862999782564884 1201 84-85 -0.31972007724450435 1201 86-87 0.07486211357229422 1201 88-89 0.2593287952740795 1201 90-91 -0.3391463517969129 1202 1 0.00861389185112671 1202 2 0.013078313385378237 1202 3 0.050598894958511664 1202 4 0.07417922809131738 1202 5 -0.06389758430027115 1202 6 -0.047402839030830535 1202 7 -0.012447753594187816 1202 8 0.020324652757551576 1202 9 0.02374327983292801 1202 10-11 -0.02034961183990447 1202 12-13 0.03387237679866928 1202 14-15 -0.09819410259814987 1202 16-17 0.007397617350299868 1202 18-19 0.12236138866677493 1202 20-21 0.02651386055375582 1202 22-23 0.020786707610071176 1202 24-25 0.007596469170472631 1202 26-27 -0.0024995020823439518 1202 28-29 0.010263674519627841 1202 30-31 -0.0590871472733312 1202 32-33 0.025824216797474264 1202 34-35 -0.05249806742236274 1202 36-37 0.019867580078056335 1202 38-39 0.008504999056789586 1202 40-41 -0.05952245780888887 1202 42-43 -0.008448321127488612 1202 44-45 0.052237373797929365 1202 46-47 0.02708026418628151 1202 48-49 0.09398895123680973 1202 50-51 0.036217566662344325 1202 52-53 -0.023332760761874738 1202 54-55 0.07194988520475931 1202 56-57 0.008201231690037503 1202 58-59 0.020833987418150457 1202 60-61 0.09679794946762854 1202 62-63 0.08123009657881397 1202 64-65 0.10469367360661153 1202 66-67 0.0918654082446082 1202 68-69 0.23858377709940015 1202 70-71 0.0496815133241455 1202 72-73 0.011276496578183526 1202 74-75 0.23537425546431479 1202 76-77 0.28110570759243103 1202 78-79 -0.10162362308236084 1202 80-81 0.10945172775332423 1202 82-83 -0.02565372285846479 1202 84-85 -0.2718341156200168 1202 86-87 -0.11377480295836051 1202 88-89 0.0775106134558996 1202 90-91 -0.8080108206613801 1203 1 0.35227425980956184 1203 2 0.23321501807423317 1203 3 0.03916322198766409 1203 4 0.028435441256462468 1203 5 0.11010224598225449 1203 6 0.04017466300167172 1203 7 0.16019214698422246 1203 8 0.09892464783026611 1203 9 0.06185087523741828 1203 10-11 -0.021519567466988576 1203 12-13 0.04833121073012592 1203 14-15 0.025067867274266575 1203 16-17 0.1551197077221218 1203 18-19 -0.02433308976679882 1203 20-21 0.0056895263526755 1203 22-23 0.02711333710313113 1203 24-25 0.01928617087832407 1203 26-27 -0.009837319241867704 1203 28-29 -0.06315063047362202 1203 30-31 0.025021001081640293 1203 32-33 -0.06879509212812707 1203 34-35 -0.056112146079328795 1203 36-37 0.0027482866995427457 1203 38-39 0.08842541611468135 1203 40-41 0.03819347111640781 1203 42-43 0.08420052978239312 1203 44-45 -0.058536711572045874 1203 46-47 0.06499000714135406 1203 48-49 0.02694757827369898 1203 50-51 -0.02465411163056075 1203 52-53 -0.07246981039166656 1203 54-55 0.050930547158543504 1203 56-57 0.16442029382174894 1203 58-59 0.06576423396325737 1203 60-61 0.013998152310286116 1203 62-63 -0.1128152652147918 1203 64-65 0.1263528609789688 1203 66-67 -0.19699744602458935 1203 68-69 0.011328475683633599 1203 70-71 -0.006141724180736219 1203 72-73 0.06137273412944211 1203 74-75 -0.06184782206348416 1203 76-77 0.15257868296150434 1203 78-79 0.07664592528985992 1203 80-81 0.24728352558282296 1203 82-83 0.3262242537246962 1203 84-85 0.2279475937743527 1203 86-87 0.3552351511947123 1203 88-89 0.29193973438656684 1203 90-91 0.4227584101078463 1204 1 0.2080887736995436 1204 2 0.1493020406470862 1204 3 -0.006711692231299082 1204 4 -0.04768049803936947 1204 5 -0.1022177447979189 1204 6 -0.005312599496164694 1204 7 -0.06023081247474238 1204 8 -0.01304593254722164 1204 9 -0.01773297540142238 1204 10-11 -0.10130667333258003 1204 12-13 0.007761886553751651 1204 14-15 -0.054760099178146504 1204 16-17 -0.06602197898195072 1204 18-19 -0.03763268692331678 1204 20-21 -0.09372742450461402 1204 22-23 -0.0766159772247903 1204 24-25 -0.049891850316797104 1204 26-27 -0.13622959135242496 1204 28-29 -0.09305534148973749 1204 30-31 -0.13556320053915982 1204 32-33 -0.18652627519247034 1204 34-35 -0.05693634290055627 1204 36-37 -0.09376508520547588 1204 38-39 -0.04439656593849861 1204 40-41 -0.040835673347011436 1204 42-43 -0.19020715421891055 1204 44-45 -0.24710196980932864 1204 46-47 -0.16287542213552086 1204 48-49 -0.16597739698012504 1204 50-51 -0.14686767082135788 1204 52-53 -0.09514012863315457 1204 54-55 -0.19920364462066686 1204 56-57 -0.10323687822742045 1204 58-59 -0.04121122793809917 1204 60-61 0.028607827482154846 1204 62-63 0.03667358210736893 1204 64-65 -0.02752206348423414 1204 66-67 -0.09647940534550514 1204 68-69 0.020315490055800467 1204 70-71 0.13515521195618163 1204 72-73 -0.1636196144353832 1204 74-75 0.07701601623051602 1204 76-77 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0.15170774273680365 2401 54-55 0.27285404843259187 2401 56-57 0.06709969484204947 2401 58-59 0.1242559682668869 2401 60-61 0.13946254821208726 2401 62-63 0.14231749044075315 2401 64-65 0.08372254806719326 2401 66-67 0.17088454654733454 2401 68-69 0.22434011749388816 2401 70-71 0.03980059091725252 2401 72-73 -0.08076729161390261 2401 74-75 0.162450425574292 2401 76-77 0.010876297015585124 2401 78-79 -0.09302971877192334 2401 80-81 0.08266280096210465 2401 82-83 0.02518757941174954 2401 84-85 0.027319323938364448 2401 86-87 -7.001312454235631E-5 2401 88-89 -0.3323378713925891 2401 90-91 -1.2579138587997178 2402 1 0.3811022960442685 2402 2 0.16387842166711408 2402 3 0.2240493995877486 2402 4 0.278041978122161 2402 5 0.22802767834511428 2402 6 0.20181962732483072 2402 7 0.15427857005915513 2402 8 0.09845126395055104 2402 9 0.14894681027664802 2402 10-11 0.11747926284311916 2402 12-13 0.06994014279916172 2402 14-15 0.08895385023617308 2402 16-17 0.06145966508034917 2402 18-19 0.08979001311567458 2402 20-21 0.01988992627273234 2402 22-23 0.04242239313771279 2402 24-25 -0.008057702689477253 2402 26-27 0.09105783032249803 2402 28-29 3.438431789604124E-4 2402 30-31 0.10533809384962467 2402 32-33 0.11548533133581884 2402 34-35 0.16822800634055568 2402 36-37 0.14229825846111055 2402 38-39 0.0706678610428142 2402 40-41 0.07990776061330251 2402 42-43 0.12472205884019871 2402 44-45 0.04623101057300261 2402 46-47 8.648038348653131E-4 2402 48-49 0.060799274520832114 2402 50-51 0.05376763932773798 2402 52-53 0.06240353039995483 2402 54-55 0.18305839947121427 2402 56-57 0.1182739823293062 2402 58-59 0.18462654943881773 2402 60-61 0.17927808451285188 2402 62-63 0.1113693867453378 2402 64-65 0.2825947465047065 2402 66-67 0.22146298073086967 2402 68-69 0.2163294617672804 2402 70-71 0.05935398046809581 2402 72-73 0.28819738809105644 2402 74-75 0.26062557104053496 2402 76-77 0.22816741124066908 2402 78-79 0.18805035445595308 2402 80-81 0.29456835329714437 2402 82-83 -0.10302525261307238 2402 84-85 -0.13520084544854427 2402 86-87 -0.7136496007228246 2402 88-89 -0.16959536698341537 2402 90-91 0.22763645888833395 2403 1 -0.15056518338398206 2403 2 -0.13722310932983817 2403 3 0.026068360837463445 2403 4 -0.0040429807343400626 2403 5 0.17125508630498842 2403 6 0.11912642911482152 2403 7 0.21114234165538903 2403 8 0.05374715697747234 2403 9 0.13548143392022638 2403 10-11 0.07581968222810076 2403 12-13 0.08771400930409357 2403 14-15 0.14901095527501695 2403 16-17 0.07162439273009369 2403 18-19 0.021967278466625828 2403 20-21 0.06431076192824037 2403 22-23 0.07735696924498825 2403 24-25 0.06094037741740266 2403 26-27 0.08555888349759755 2403 28-29 0.019846279414736046 2403 30-31 0.023289043346395033 2403 32-33 0.04219722990106334 2403 34-35 0.13834570854029238 2403 36-37 0.07486660734667794 2403 38-39 0.009851570236598661 2403 40-41 0.13604281152812092 2403 42-43 0.16337072041091716 2403 44-45 0.18121187736199573 2403 46-47 0.13857078170580905 2403 48-49 8.308044030229667E-4 2403 50-51 0.1619036209449476 2403 52-53 0.13108226094249886 2403 54-55 -0.06937974214474707 2403 56-57 0.18377729134787302 2403 58-59 0.07525044999200503 2403 60-61 0.03782627330644317 2403 62-63 0.22857480716145062 2403 64-65 0.26624902700604736 2403 66-67 0.29948948216010507 2403 68-69 0.2767445055776321 2403 70-71 0.13072899178476405 2403 72-73 0.2671936563414974 2403 74-75 0.2813643959881773 2403 76-77 0.25779320109386106 2403 78-79 0.2069312559391605 2403 80-81 0.20676522147241627 2403 82-83 0.03449940347581304 2403 84-85 -0.11352072642146993 2403 86-87 0.038364820650095055 2403 88-89 -0.03596989684328378 2403 90-91 0.4227584101078463 2404 1 -0.558267291512621 2404 2 -0.34005927038327144 2404 3 -0.3647465606616507 2404 4 -0.3876052749860506 2404 5 -0.1718499719376041 2404 6 -0.22531599301736804 2404 7 -0.06314782674166963 2404 8 -0.1466318946811782 2404 9 -0.04505082108104119 2404 10-11 0.017492020161753885 2404 12-13 0.10684068694401105 2404 14-15 0.041543083661949254 2404 16-17 -0.0027872462924420915 2404 18-19 0.07926422073049366 2404 20-21 0.03122485410347764 2404 22-23 -0.001828425791728705 2404 24-25 -0.08767517398801772 2404 26-27 -0.009698618401614567 2404 28-29 0.01732388450463418 2404 30-31 -0.016932769137781634 2404 32-33 0.07722636891138279 2404 34-35 0.05881447734416412 2404 36-37 -0.004015558325420443 2404 38-39 0.05158087334354633 2404 40-41 0.09738435707063076 2404 42-43 0.09177010940487662 2404 44-45 -0.06825536716527125 2404 46-47 0.004036616194476039 2404 48-49 0.11365334913337222 2404 50-51 0.10421732262278027 2404 52-53 0.1040663253152232 2404 54-55 0.023709925360122952 2404 56-57 0.05490881923270763 2404 58-59 0.06348327526588093 2404 60-61 0.20558552666012986 2404 62-63 0.03418395588077061 2404 64-65 0.1203671981922767 2404 66-67 0.12949526380343457 2404 68-69 -0.010170691777609875 2404 70-71 0.03831109354543116 2404 72-73 0.11206827968354105 2404 74-75 0.12052776633571938 2404 76-77 0.1116599743409381 2404 78-79 -0.08185632165297108 2404 80-81 -0.08460428998112235 2404 82-83 0.34981198543743375 2404 84-85 0.01914879538436054 2404 86-87 0.19963955783255471 2404 88-89 -0.06553892959364305 2404 90-91 0.17275841010784632 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 17.0 31 449.0 32 3018.0 33 5552.0 34 7461.0 35 9167.0 36 13654.0 37 21824.0 38 71534.0 39 367865.0 40 216489.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.304551565629236 12.030910926769945 32.80206630703403 28.862471200566787 2 27.387969148802632 17.3444538975439 29.779076416686422 25.48850053696704 3 26.867070468146593 19.549133286565628 22.563418821537503 31.02037742375028 4 26.592611187816413 20.974436216057907 21.07666345899056 31.35628913713513 5 26.620085631005676 21.55265470064014 21.190884621285022 30.63637504706916 6 27.378352370193713 23.536253713233755 23.983654798265068 25.101739118307464 7 25.71872864454765 23.563309763887148 23.818808139129466 26.89915345243574 8 24.732856365842434 24.827273614772047 24.42115392661395 26.01871609277157 9 24.5667545290992 24.998396161945806 24.791849713401113 25.642999595553885 10-11 24.362021114876644 23.79105476758295 24.73745868373708 27.109465433803326 12-13 24.72379119423176 23.506268914829224 24.56989247311828 27.200047417820734 14-15 25.036121222264065 23.109493326639054 24.53453830383666 27.31984714726023 16-17 25.089326806409773 22.901831164665357 24.314324365786646 27.694517663138225 18-19 25.232905178304954 23.10719216769173 24.20414766467233 27.455754989330988 20-21 25.145321674128002 23.187872195026706 24.277854483076023 27.38895164776927 22-23 24.96562207996876 22.796187049356373 24.51696581732982 27.72122505334505 24-25 25.02147748350836 23.051267032062814 24.520940546420654 27.406314938008176 26-27 25.16693862181499 23.137037501917632 24.555388198541205 27.140635677726177 28-29 25.12000892570743 22.85148459618147 24.57282122086942 27.45568525724168 30-31 25.316392142861627 23.03259489323661 24.5084058605678 27.142607103333965 32-33 25.356551089072326 23.07241294946479 24.539202966105623 27.03183299535726 34-35 25.134289427833355 22.784796120544033 24.804337980045055 27.276576471577556 36-37 25.299758628438894 22.971485635439205 24.75067092396911 26.97808481215279 38-39 25.386071455475363 22.977242019320002 24.736381514730418 26.900305010474217 40-41 25.26791732620402 22.803877364341478 24.715715207774252 27.212490101680253 42-43 25.43665309907775 22.934525575586157 24.791733660708417 26.837087664627678 44-45 25.378585695505635 23.034362699147675 24.742407067561683 26.844644537785012 46-47 25.284043177178756 22.8219499754012 24.815509014025842 27.0784978333942 48-49 25.515301520441586 22.93080976569749 24.73199142027284 26.821897293588076 50-51 25.518896555573384 22.995402314889105 24.65122011911656 26.83448101042095 52-53 25.52405900067946 22.79777984794757 24.696733431743432 26.981427719629536 54-55 25.69943160662837 22.981118128822093 24.66266670880041 26.656783555749126 56-57 25.698689660671914 22.959450045664024 24.64262447019952 26.69923582346454 58-59 25.710190317582626 22.80082523427208 24.625951795050252 26.863032653095043 60-61 25.70587976184358 23.024234103940152 24.55217196596507 26.717714168251195 62-63 25.92728840820514 22.824303141407267 24.473996685080614 26.774411765306976 64-65 25.778769966330273 22.700362367836586 24.479654913969153 27.04121275186398 66-67 25.99573615866658 22.919310032947866 24.266683894308418 26.818269914077135 68-69 26.153064610379122 22.77910080487683 24.16950277346285 26.898331811281196 70-71 25.924240892393758 22.686280456981095 24.102382637414486 27.287096013210665 72-73 26.135018690749153 22.83357225722533 24.042514130362605 26.988894921662908 74-75 26.202368078593818 22.673161164977365 23.96167451749482 27.162796238934 76-77 26.136610951510402 22.602180726038128 23.996691912596848 27.264516409854618 78-79 26.487045131391856 22.670361788403813 23.808520722523564 27.03407235768076 80-81 26.946361841353767 22.48331456732103 23.374292194970707 27.1960313963545 82-83 26.942708333333332 22.289583333333333 23.211458333333333 27.55625 84-85 27.534089958450686 21.72833997936477 23.022224700928582 27.71534536125596 86-87 27.887573131747722 20.967450089467018 22.372785942620478 28.77219083616478 88-89 27.445192530927663 20.317734071838416 22.155152399588737 30.081920997645184 90-91 23.74242175979027 21.19449451089628 25.094215959364245 29.968867769949203 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 4.5 10 7.5 11 10.0 12 15.0 13 25.5 14 39.5 15 57.0 16 80.0 17 113.0 18 160.5 19 222.0 20 313.5 21 447.0 22 611.0 23 835.0 24 1154.5 25 1473.5 26 1838.0 27 2350.0 28 2939.5 29 3540.5 30 4283.0 31 5209.5 32 6237.5 33 7389.5 34 8794.5 35 10385.5 36 12186.0 37 14116.5 38 16350.0 39 19008.5 40 21817.0 41 24844.5 42 27896.0 43 31122.0 44 34625.5 45 38127.0 46 41771.5 47 45517.0 48 48553.0 49 51082.5 50 53644.5 51 55882.0 52 57186.5 53 57302.0 54 56611.0 55 55414.0 56 53691.5 57 51443.0 58 48940.0 59 46239.5 60 43382.5 61 40370.5 62 37418.0 63 34291.0 64 30831.0 65 27564.0 66 24312.5 67 21434.5 68 18796.5 69 16038.5 70 13581.0 71 11349.0 72 9246.0 73 7278.5 74 5639.0 75 4469.0 76 3493.5 77 2556.0 78 1848.5 79 1348.5 80 958.5 81 683.5 82 484.5 83 323.5 84 212.5 85 151.0 86 113.0 87 80.5 88 59.5 89 39.0 90 21.5 91 14.0 92 13.5 93 11.0 94 7.5 95 4.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.7892835725144E-4 2 0.005578567145028799 3 0.0015341059648829199 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10449.0 32-33 22685.0 34-35 25270.0 36-37 27464.0 38-39 29319.0 40-41 31029.0 42-43 32217.0 44-45 33264.0 46-47 33613.0 48-49 33266.0 50-51 33454.0 52-53 33079.0 54-55 31680.0 56-57 31038.0 58-59 29641.0 60-61 28074.0 62-63 26916.0 64-65 25194.0 66-67 23427.0 68-69 22232.0 70-71 20810.0 72-73 19261.0 74-75 17568.0 76-77 16307.0 78-79 15018.0 80-81 13594.0 82-83 12455.0 84-85 11232.0 86-87 10065.0 88-89 9206.0 90-91 8203.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80858814793991 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81889569813353 99.6278305011718 2 0.17228327116891035 0.3439070011375524 3 0.007702503167337401 0.02306327899010944 4 6.187054271180186E-4 0.0024700846064047025 5 2.6456462005338836E-4 0.0013202910600712422 6 2.3525748305107397E-4 0.0014088430340739358 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012551776076314798 0.0 0.0 0.0 0.0 2 0.0012551776076314798 0.0 0.0 0.0 1.3946417862572E-4 3 0.0012551776076314798 0.0 0.0 0.0 1.3946417862572E-4 4 0.0012551776076314798 0.0 0.0 0.0 1.3946417862572E-4 5 0.0012551776076314798 0.0 0.0 0.0 1.3946417862572E-4 6 0.0012551776076314798 0.0 0.0 0.0 1.3946417862572E-4 7 0.0013946417862571998 0.0 0.0 0.0 1.3946417862572E-4 8 0.0046023178946487595 0.0 0.0 0.0 1.3946417862572E-4 9 0.004881246251900199 0.0 0.0 0.0 1.3946417862572E-4 10-11 0.004881246251900199 0.0 0.0 0.0 1.3946417862572E-4 12-13 0.004881246251900199 0.0 0.0 0.0 1.3946417862572E-4 14-15 0.005020710430525919 0.0 0.0 0.0 1.3946417862572E-4 16-17 0.005020710430525919 0.0 6.973208931286E-5 0.0 1.3946417862572E-4 18-19 0.005020710430525919 0.0 1.3946417862572E-4 0.0 1.3946417862572E-4 20-21 0.00536937087709022 0.0 1.3946417862572E-4 0.0 1.3946417862572E-4 22-23 0.005857495502280239 0.0 1.3946417862572E-4 0.0 1.3946417862572E-4 24-25 0.006206155948844539 0.0 1.3946417862572E-4 0.0 2.0919626793858E-4 26-27 0.006415352216783119 0.0 1.3946417862572E-4 0.0 4.1839253587715994E-4 28-29 0.007252137288537439 0.0 1.3946417862572E-4 0.0 4.1839253587715994E-4 30-31 0.0080191902709789 0.0 1.3946417862572E-4 0.0 4.1839253587715994E-4 32-33 0.008367850717543198 0.0 1.3946417862572E-4 1.3946417862572E-4 4.1839253587715994E-4 34-35 0.009065171610671798 0.0 1.3946417862572E-4 1.3946417862572E-4 4.1839253587715994E-4 36-37 0.00969276041448754 0.0 1.3946417862572E-4 1.3946417862572E-4 4.881246251900199E-4 38-39 0.010111152950364698 0.0 1.3946417862572E-4 1.3946417862572E-4 5.5785671450288E-4 40-41 0.010459813396928998 0.0 1.3946417862572E-4 1.3946417862572E-4 6.973208931285999E-4 42-43 0.01129659846868332 0.0 1.3946417862572E-4 1.3946417862572E-4 6.973208931285999E-4 44-45 0.01129659846868332 0.0 1.3946417862572E-4 1.3946417862572E-4 6.973208931285999E-4 46-47 0.011366330557996178 0.0 1.3946417862572E-4 1.3946417862572E-4 8.367850717543199E-4 48-49 0.01164525891524762 0.0 1.3946417862572E-4 1.3946417862572E-4 8.367850717543199E-4 50-51 0.011854455183186199 0.0 1.3946417862572E-4 1.3946417862572E-4 8.367850717543199E-4 52-53 0.01199391936181192 0.0 1.3946417862572E-4 1.3946417862572E-4 9.065171610671799E-4 54-55 0.01234257980837622 0.0 1.3946417862572E-4 1.3946417862572E-4 9.762492503800399E-4 56-57 0.012482043987001938 0.0 1.3946417862572E-4 1.3946417862572E-4 9.762492503800399E-4 58-59 0.012760972344253378 0.0 1.3946417862572E-4 1.3946417862572E-4 9.762492503800399E-4 60-61 0.013109632790817678 0.0 1.3946417862572E-4 1.3946417862572E-4 9.762492503800399E-4 62-63 0.013388561148069118 0.0 1.3946417862572E-4 1.3946417862572E-4 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