##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-19A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 714992 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.24962796786537 40.0 40.0 40.0 40.0 40.0 2 39.12390907870298 40.0 40.0 40.0 40.0 40.0 3 39.1165075972878 40.0 40.0 40.0 40.0 40.0 4 39.038014411350055 40.0 40.0 40.0 40.0 40.0 5 39.199515519054756 40.0 40.0 40.0 40.0 40.0 6 39.23238861413834 40.0 40.0 40.0 40.0 40.0 7 39.27883388905051 40.0 40.0 40.0 40.0 40.0 8 39.32124555239779 40.0 40.0 40.0 40.0 40.0 9 39.328221854230534 40.0 40.0 40.0 40.0 40.0 10-11 39.337889095262604 40.0 40.0 40.0 40.0 40.0 12-13 39.32514209949203 40.0 40.0 40.0 40.0 40.0 14-15 39.34436469219236 40.0 40.0 40.0 40.0 40.0 16-17 39.4752976257077 40.0 40.0 40.0 40.0 40.0 18-19 39.484503323114104 40.0 40.0 40.0 40.0 40.0 20-21 39.49977062680422 40.0 40.0 40.0 40.0 40.0 22-23 39.49805871953812 40.0 40.0 40.0 40.0 40.0 24-25 39.49504609841789 40.0 40.0 40.0 40.0 40.0 26-27 39.482343858392824 40.0 40.0 40.0 40.0 40.0 28-29 39.48127251773447 40.0 40.0 40.0 40.0 40.0 30-31 39.46830787873536 40.0 40.0 40.0 40.0 40.0 32-33 39.4716292066698 40.0 40.0 40.0 40.0 40.0 34-35 39.46885405624164 40.0 40.0 40.0 40.0 40.0 36-37 39.464341823971964 40.0 40.0 40.0 40.0 40.0 38-39 39.446006105550545 40.0 40.0 40.0 40.0 40.0 40-41 39.430554911434896 40.0 40.0 40.0 40.0 40.0 42-43 39.434782085006034 40.0 40.0 40.0 40.0 40.0 44-45 39.40720672136307 40.0 40.0 40.0 40.0 40.0 46-47 39.40940053064588 40.0 40.0 40.0 40.0 40.0 48-49 39.40927339600083 40.0 40.0 40.0 40.0 40.0 50-51 39.401897397051 40.0 40.0 40.0 40.0 40.0 52-53 39.39118667116766 40.0 40.0 40.0 40.0 40.0 54-55 39.36864244989941 40.0 40.0 40.0 40.0 40.0 56-57 39.36706693510135 40.0 40.0 40.0 40.0 40.0 58-59 39.353304966674365 40.0 40.0 40.0 40.0 40.0 60-61 39.31408016725375 40.0 40.0 40.0 40.0 40.0 62-63 39.372587101538215 40.0 40.0 40.0 40.0 40.0 64-65 39.36691533165548 40.0 40.0 40.0 40.0 40.0 66-67 39.357754074617866 40.0 40.0 40.0 40.0 40.0 68-69 39.3549057449386 40.0 40.0 40.0 40.0 40.0 70-71 39.35701165645743 40.0 40.0 40.0 40.0 40.0 72-73 39.349019403843414 40.0 40.0 40.0 40.0 40.0 74-75 39.350619048378434 40.0 40.0 40.0 40.0 40.0 76-77 39.353000808866824 40.0 40.0 40.0 40.0 40.0 78-79 39.32598398696098 40.0 40.0 40.0 40.0 40.0 80-81 39.32541920942667 40.0 40.0 40.0 40.0 40.0 82-83 39.29514368059606 40.0 40.0 40.0 40.0 40.0 84-85 39.327885982937644 40.0 40.0 40.0 40.0 40.0 86-87 39.32672690838632 40.0 40.0 40.0 40.0 40.0 88-89 39.37781564207954 40.0 40.0 40.0 40.0 40.0 90-91 39.428724065657526 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1510819385184874 1101 2 0.08644993209649954 1101 3 0.22458575280801085 1101 4 0.2915367152777293 1101 5 0.05861418381081762 1101 6 0.009367105503152118 1101 7 -0.011962763358219775 1101 8 0.033281251395514744 1101 9 0.10330147361040787 1101 10-11 0.11738507656521335 1101 12-13 0.08130868883721831 1101 14-15 0.03884814499342326 1101 16-17 -0.05886086902360432 1101 18-19 0.02426584290757461 1101 20-21 0.057923657849528354 1101 22-23 -0.006868242502633848 1101 24-25 0.05515990327818798 1101 26-27 0.11463145410196063 1101 28-29 0.024084717711353676 1101 30-31 0.016400009419804462 1101 32-33 0.050917491326202935 1101 34-35 -0.0054302357833151405 1101 36-37 0.10487396513473612 1101 38-39 0.06886446770248966 1101 40-41 0.06183154772224242 1101 42-43 -0.03845021316082864 1101 44-45 0.08428124461730135 1101 46-47 0.08724705129014154 1101 48-49 0.010924096561396368 1101 50-51 0.06429269775724578 1101 52-53 0.04295165576112936 1101 54-55 0.005102736485405046 1101 56-57 0.1027074060679567 1101 58-59 0.2585293774004356 1101 60-61 0.15614694194749035 1101 62-63 -0.04573135023504449 1101 64-65 -0.04690500403264508 1101 66-67 -0.06241775111570291 1101 68-69 -0.01879851876427807 1101 70-71 0.1021596719198925 1101 72-73 0.32697106919895447 1101 74-75 0.038007553724149545 1101 76-77 0.1655799805578937 1101 78-79 0.3334633735211625 1101 80-81 0.0802323550710966 1101 82-83 0.513882719356566 1101 84-85 0.19246833007117203 1101 86-87 0.45587071764582277 1101 88-89 -0.024581303437685165 1101 90-91 0.716033566958302 1102 1 0.3174905848345162 1102 2 0.192281889844665 1102 3 0.021687202083370494 1102 4 -0.08406415462942363 1102 5 0.027206530149150865 1102 6 0.05821756290920632 1102 7 0.059305981497516314 1102 8 0.07760283232929055 1102 9 0.06017875396775452 1102 10-11 -0.06936304009557404 1102 12-13 -0.14797109070628522 1102 14-15 -0.08238548187949846 1102 16-17 0.03506132990293054 1102 18-19 -0.1093667172718753 1102 20-21 0.020660564668759207 1102 22-23 0.032299211832651054 1102 24-25 -0.006219980485298038 1102 26-27 -0.08150797528053744 1102 28-29 0.007344622535164547 1102 30-31 -0.03170168304715304 1102 32-33 0.033881679817483246 1102 34-35 0.03468936524316035 1102 36-37 -0.04928738453677539 1102 38-39 0.02382849409740828 1102 40-41 0.0323169924785347 1102 42-43 0.005731273256884606 1102 44-45 -0.005403782417126024 1102 46-47 0.048463816630679446 1102 48-49 -0.03513495797342614 1102 50-51 0.12562221736600776 1102 52-53 -0.01788008737393909 1102 54-55 0.030255499682262155 1102 56-57 -0.14428715713215468 1102 58-59 -0.06612612733394485 1102 60-61 0.003165139320678634 1102 62-63 -0.16168181214260358 1102 64-65 -0.23589225723404894 1102 66-67 -0.30018851062293095 1102 68-69 -0.170421242002611 1102 70-71 -0.12163344364348205 1102 72-73 -0.06373112105974599 1102 74-75 0.010917534244875071 1102 76-77 -0.29852499320102055 1102 78-79 -0.17461377530197808 1102 80-81 -0.11433721038454792 1102 82-83 -0.0987545661002045 1102 84-85 -0.2706086434404966 1102 86-87 -0.08118229962198598 1102 88-89 -0.4317077576229593 1102 90-91 -1.5471243277785405 1103 1 0.3519161175823271 1103 2 0.2376294167372066 1103 3 0.17528997816012293 1103 4 0.05797070291768591 1103 5 0.12303822070942516 1103 6 0.2075155745246633 1103 7 0.10178180127621772 1103 8 0.05095495619018209 1103 9 0.09522784142479424 1103 10-11 0.0788486441452676 1103 12-13 0.04620468461269667 1103 14-15 0.09643001583180677 1103 16-17 0.1302547856543157 1103 18-19 0.06913700308481197 1103 20-21 0.038354279069523045 1103 22-23 0.08378581659124507 1103 24-25 0.10363470050914714 1103 26-27 -0.038662356800948317 1103 28-29 0.03258740342139532 1103 30-31 0.055377871908149245 1103 32-33 0.1468999890594347 1103 34-35 0.09556227238233106 1103 36-37 0.0794924605561107 1103 38-39 0.06455814498519175 1103 40-41 0.04931408858747233 1103 42-43 0.06093270334326917 1103 44-45 0.058598702400594505 1103 46-47 -0.016266157563329386 1103 48-49 -0.014649595579797392 1103 50-51 0.09611564583573795 1103 52-53 0.11479344791960244 1103 54-55 0.09448666855133325 1103 56-57 0.11803606954709522 1103 58-59 -0.021723607821016344 1103 60-61 -0.16720630552095628 1103 62-63 -0.29283073374723756 1103 64-65 -0.41261340481092645 1103 66-67 -0.30073041944501 1103 68-69 -0.5012761681533178 1103 70-71 -0.1241078010834471 1103 72-73 -0.12075899854288963 1103 74-75 -0.1125965261309716 1103 76-77 -0.248344024533381 1103 78-79 -0.10324075525844023 1103 80-81 -0.3816737830625314 1103 82-83 -0.424006902548804 1103 84-85 -0.49007082342738784 1103 86-87 -0.3877135806554506 1103 88-89 -0.2318214262788274 1103 90-91 0.716033566958302 1104 1 0.26897710289015464 1104 2 0.1324658900886675 1104 3 0.15518038796088973 1104 4 0.011618117323457966 1104 5 0.27011312936224385 1104 6 0.11907459286279476 1104 7 0.13820574731599322 1104 8 -0.016262831873781636 1104 9 -0.07395929414420266 1104 10-11 0.03796764250986939 1104 12-13 0.027511206920429743 1104 14-15 0.029775634469537238 1104 16-17 0.03385807864430035 1104 18-19 -0.042181725267290915 1104 20-21 -0.044827139209431266 1104 22-23 0.02216642853149864 1104 24-25 -0.006380860876426198 1104 26-27 0.061899464700182705 1104 28-29 0.036052387661861474 1104 30-31 -0.10126636417049184 1104 32-33 0.004601927040127407 1104 34-35 -0.0032938320872446525 1104 36-37 0.08883555845373792 1104 38-39 0.0028636468119174197 1104 40-41 -0.1334049057957074 1104 42-43 -0.0366540140794811 1104 44-45 -0.08986875433224384 1104 46-47 -0.05502991765570897 1104 48-49 -0.0035665099485342466 1104 50-51 0.04156414054268254 1104 52-53 0.059368889832271066 1104 54-55 -0.16041687470487176 1104 56-57 -0.069024859106122 1104 58-59 -0.04821527731489539 1104 60-61 0.016088686668105368 1104 62-63 0.0323267565628953 1104 64-65 0.07396233076485004 1104 66-67 -0.02336732167562161 1104 68-69 -0.061670946228694845 1104 70-71 0.04464951261581973 1104 72-73 0.07134558812433767 1104 74-75 0.13470185737003249 1104 76-77 -0.07829088045520649 1104 78-79 0.042666240971136915 1104 80-81 0.013460388299129988 1104 82-83 0.17083201581061758 1104 84-85 0.19283723040420142 1104 86-87 -0.04243039162226836 1104 88-89 0.37449205563568455 1104 90-91 0.4303192812440173 1201 1 0.30597869254548726 1201 2 0.38008117011730747 1201 3 0.2287032897722625 1201 4 0.11480826582084092 1201 5 0.11184020724966359 1201 6 0.07567267358543006 1201 7 0.17543483683940053 1201 8 0.05937455482259679 1201 9 0.033313213097088124 1201 10-11 0.09666450430717788 1201 12-13 -0.04512318151727612 1201 14-15 0.041310509725356326 1201 16-17 -0.06610915505525838 1201 18-19 -0.006068017041563678 1201 20-21 0.08106054473764601 1201 22-23 0.035595872133171724 1201 24-25 -0.048990954224045424 1201 26-27 0.04945016636349209 1201 28-29 0.038279990536828734 1201 30-31 0.032691877596782604 1201 32-33 0.037432691346531044 1201 34-35 -0.022752982878081696 1201 36-37 -0.03350653838841566 1201 38-39 -0.0023457522290826205 1201 40-41 0.030238739120214575 1201 42-43 -0.05715697958316923 1201 44-45 0.04482866140152453 1201 46-47 -0.08870650190338125 1201 48-49 0.021413888459576924 1201 50-51 0.0601672019123356 1201 52-53 0.0691250033684554 1201 54-55 -0.08755266720887533 1201 56-57 0.060005505769559875 1201 58-59 -0.004805017456320115 1201 60-61 -0.014490757041336622 1201 62-63 -0.1271343156597311 1201 64-65 -0.0771059396832996 1201 66-67 -0.06986289356346731 1201 68-69 -0.17010195811842976 1201 70-71 -0.2427892885817542 1201 72-73 -0.06921090292119203 1201 74-75 0.029925697331250944 1201 76-77 -0.1260574745073697 1201 78-79 -0.053607445389467046 1201 80-81 0.048444597982133075 1201 82-83 -0.388944393769485 1201 84-85 -0.06617152707638496 1201 86-87 0.0769575297783689 1201 88-89 -0.6756620059889684 1201 90-91 0.716033566958302 1202 1 0.04179556615159896 1202 2 0.18322417747570796 1202 3 0.06185033499853887 1202 4 0.20442614060873865 1202 5 0.12328545286814574 1202 6 0.20193997427846 1202 7 0.08033827450947939 1202 8 0.06007679271306898 1202 9 0.03525730325704046 1202 10-11 0.010101364342844477 1202 12-13 0.017823205391778174 1202 14-15 0.04362727876578276 1202 16-17 0.010007888289138123 1202 18-19 0.07557083371533224 1202 20-21 -0.03859093582930484 1202 22-23 0.03645888554068222 1202 24-25 0.06727869650192275 1202 26-27 0.0317402195641705 1202 28-29 -0.06453749259944885 1202 30-31 0.013745006513183 1202 32-33 -0.032420234179618035 1202 34-35 0.040618669565823495 1202 36-37 0.06429547683553238 1202 38-39 0.04144953790626715 1202 40-41 0.036931673830160605 1202 42-43 0.17269680845087976 1202 44-45 0.12288301445357774 1202 46-47 0.08247387902935799 1202 48-49 0.011560933235543303 1202 50-51 0.12971596202066138 1202 52-53 0.08835709472865005 1202 54-55 0.17999576367574832 1202 56-57 0.18255079515493122 1202 58-59 0.03854491270969618 1202 60-61 0.053193469545028904 1202 62-63 0.07774384713388827 1202 64-65 -0.14267914679711424 1202 66-67 0.029414998745565413 1202 68-69 -6.252037337119987E-4 1202 70-71 0.026579551103097288 1202 72-73 0.0016000850808737255 1202 74-75 0.04788127689369048 1202 76-77 0.06432579597868227 1202 78-79 0.1347938319486417 1202 80-81 0.09893475486386905 1202 82-83 0.1126991785322744 1202 84-85 0.1943532998255506 1202 86-87 0.1919068008264091 1202 88-89 0.07876216160791216 1202 90-91 -0.45107518635735033 1203 1 0.182799525746006 1203 2 0.3351343883426807 1203 3 0.19692927536986815 1203 4 0.050918198663630676 1203 5 -0.030599764406510133 1203 6 -0.022223851844323406 1203 7 -0.03881725155605409 1203 8 0.08268097460049972 1203 9 0.07558743713588711 1203 10-11 -0.057603987217873964 1203 12-13 0.06368730962037716 1203 14-15 -0.026460895868382295 1203 16-17 0.028905789201076004 1203 18-19 0.06801454782925731 1203 20-21 0.04050693298427177 1203 22-23 0.0736785939248179 1203 24-25 -0.003634094209679972 1203 26-27 -0.052111318401706797 1203 28-29 -0.07363581653883244 1203 30-31 -0.010944370132158099 1203 32-33 0.06882223686807265 1203 34-35 -0.013319689288145753 1203 36-37 0.0037942793887211224 1203 38-39 -0.0565443346517327 1203 40-41 -0.032111299910631885 1203 42-43 -0.0253174516563206 1203 44-45 -0.03989630962639268 1203 46-47 -0.10817484977864922 1203 48-49 -0.08502685832948487 1203 50-51 -0.12650547641082 1203 52-53 0.018473637574928148 1203 54-55 -0.06628341711111574 1203 56-57 -0.10359654864205226 1203 58-59 -0.06949957510515503 1203 60-61 -0.18899463639288427 1203 62-63 -0.13847065609779463 1203 64-65 -0.04184032431544438 1203 66-67 0.08152387356027191 1203 68-69 -0.002234507947811437 1203 70-71 -0.005177236171249433 1203 72-73 0.12995290079319943 1203 74-75 -0.07263485000541436 1203 76-77 0.22505108353239933 1203 78-79 -0.060615930928911155 1203 80-81 0.1273333745859091 1203 82-83 0.3962587521001453 1203 84-85 0.5462441274445311 1203 86-87 0.14352205927583128 1203 88-89 0.44368363924586873 1203 90-91 0.1006489515736888 1204 1 0.2765955448273303 1204 2 0.06532545666949119 1204 3 -0.051923363849809334 1204 4 0.015980222239313946 1204 5 -0.17169673175629896 1204 6 -0.1148174321897244 1204 7 0.05380995533065658 1204 8 -0.05034678237004897 1204 9 0.026352382973968247 1204 10-11 -0.06012771712022413 1204 12-13 0.006221289820139475 1204 14-15 -0.09374760503590096 1204 16-17 -0.05222983707587048 1204 18-19 -0.05841081168484408 1204 20-21 -0.01803049799434575 1204 22-23 -0.05174608610124665 1204 24-25 -0.07650686761084557 1204 26-27 0.0143353070009411 1204 28-29 -0.03610105945001152 1204 30-31 -0.05752756567397199 1204 32-33 -0.07535418057349119 1204 34-35 -0.09430469445774037 1204 36-37 -0.1056807524903789 1204 38-39 -0.10326085774940452 1204 40-41 -0.02273340728462614 1204 42-43 -0.047392962578939546 1204 44-45 -0.05568976596295272 1204 46-47 0.0798415013710212 1204 48-49 -0.0556322671305054 1204 50-51 -0.05605622162291013 1204 52-53 -0.022342466423495466 1204 54-55 -0.095919099907114 1204 56-57 0.018817711564786066 1204 58-59 -0.08538478188876297 1204 60-61 -0.17479848216550664 1204 62-63 -0.047731009100914434 1204 64-65 0.01943491077855697 1204 66-67 -0.11115792291403181 1204 68-69 -0.004731104403475683 1204 70-71 -0.012874191183506412 1204 72-73 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48-49 0.07078925450313989 2401 50-51 0.14281231706338104 2401 52-53 0.18891804145413005 2401 54-55 0.11905812318552478 2401 56-57 0.17667677591395403 2401 58-59 0.14567859299808106 2401 60-61 0.13238203796979775 2401 62-63 -0.03544064602220942 2401 64-65 0.20363624596832608 2401 66-67 0.2917336943031046 2401 68-69 0.13527035400885978 2401 70-71 0.1679560096581767 2401 72-73 -0.11857146647467687 2401 74-75 0.20167059972376222 2401 76-77 0.14615672153091452 2401 78-79 0.17328749636415353 2401 80-81 0.14832320813810895 2401 82-83 0.11952740098549697 2401 84-85 -0.12543788772340037 2401 86-87 0.12737991171076146 2401 88-89 -0.32265080150718006 2401 90-91 -0.4696807187559813 2402 1 0.30689015186781887 2402 2 0.3503909669612 2402 3 0.2904893254753702 2402 4 0.3687556344981502 2402 5 0.23301755561584514 2402 6 0.21580603878089022 2402 7 0.20942837443700313 2402 8 0.27141193322408697 2402 9 0.14247176280949958 2402 10-11 0.1413544455161002 2402 12-13 0.17161444459470943 2402 14-15 0.15995563712809258 2402 16-17 -0.0384673982992112 2402 18-19 0.06571602630494766 2402 20-21 0.06892567134966754 2402 22-23 0.021570694432689663 2402 24-25 0.0025711565677326575 2402 26-27 0.06376786797468981 2402 28-29 0.004826317307070838 2402 30-31 0.0032314868339184954 2402 32-33 0.04482475992338664 2402 34-35 0.01571841462959611 2402 36-37 -0.08758111139639624 2402 38-39 0.051525919919335195 2402 40-41 -0.052158890457924656 2402 42-43 0.0024070841860179826 2402 44-45 0.08956187662357706 2402 46-47 0.11610337201857135 2402 48-49 0.04426312162174639 2402 50-51 0.1518564483951721 2402 52-53 0.051117325268677405 2402 54-55 0.03993578876016102 2402 56-57 0.007898967641338572 2402 58-59 0.15038020423849474 2402 60-61 0.19510619424577413 2402 62-63 0.19222003288250278 2402 64-65 0.18100586516833062 2402 66-67 0.10491043661398436 2402 68-69 0.16733726376673275 2402 70-71 0.11977928539244687 2402 72-73 0.25492134565079994 2402 74-75 0.19112468710441988 2402 76-77 0.048572869417604636 2402 78-79 0.04869157341917685 2402 80-81 0.2919229285171525 2402 82-83 0.35385016501734157 2402 84-85 -0.19625145922283593 2402 86-87 -0.1633559565753515 2402 88-89 0.26902839648781196 2402 90-91 0.5109053618300976 2403 1 -0.3184393485477912 2403 2 -0.12823242095561227 2403 3 -0.12685623243679345 2403 4 0.05562328507730996 2403 5 0.19547282835529955 2403 6 0.20952764695927328 2403 7 0.10278783749352272 2403 8 0.19314140823578896 2403 9 0.14184085282654024 2403 10-11 0.12143751067779363 2403 12-13 0.16525230366030996 2403 14-15 0.19820734648237703 2403 16-17 0.08749824641890314 2403 18-19 0.07869434714920231 2403 20-21 0.06937372910305584 2403 22-23 0.11732035716803324 2403 24-25 0.08552604859767854 2403 26-27 0.13990907177048229 2403 28-29 0.029569294769480337 2403 30-31 0.05940201677736212 2403 32-33 0.08198605397734582 2403 34-35 0.10566936682783279 2403 36-37 0.09890533833191029 2403 38-39 0.13883703982811113 2403 40-41 0.12472871864282808 2403 42-43 0.15091570861582682 2403 44-45 0.1302007176980453 2403 46-47 0.051107715934001874 2403 48-49 0.1848427147480436 2403 50-51 0.1044941239687418 2403 52-53 0.06213382407490542 2403 54-55 0.12201172144282424 2403 56-57 0.07002455172544586 2403 58-59 -0.019101938405924557 2403 60-61 0.08493285151918428 2403 62-63 0.1518674860403948 2403 64-65 0.2605143940766226 2403 66-67 0.36875289714483017 2403 68-69 0.3071136367442264 2403 70-71 0.2718180907034835 2403 72-73 0.2715853906290491 2403 74-75 0.29765516620308574 2403 76-77 0.32942296524289816 2403 78-79 0.39205811254042544 2403 80-81 0.36887640808418354 2403 82-83 0.3130081651605181 2403 84-85 0.29700415312188966 2403 86-87 0.20502544555131408 2403 88-89 0.3022609330511159 2403 90-91 0.480739449311244 2404 1 -0.3416266004011561 2404 2 -0.19704458090281918 2404 3 -0.3373572061937722 2404 4 -0.2969789594056351 2404 5 -0.18753645730518542 2404 6 -0.14546953757395187 2404 7 -0.23875069853548325 2404 8 -0.11278057643515638 2404 9 -0.03012678185857709 2404 10-11 0.08049936845547734 2404 12-13 0.05402836466089411 2404 14-15 0.050037130892945925 2404 16-17 0.015425883542896202 2404 18-19 0.030093921063269136 2404 20-21 0.007332587422311576 2404 22-23 -0.005058299800516863 2404 24-25 0.004763846149479889 2404 26-27 -0.019252223989823847 2404 28-29 0.0024914982219215176 2404 30-31 -0.05863194456645715 2404 32-33 0.061228804697620376 2404 34-35 -0.0142187386000856 2404 36-37 0.023810586376747267 2404 38-39 0.07294196475016435 2404 40-41 0.09520187055487384 2404 42-43 0.022493728747107866 2404 44-45 0.05233469978742278 2404 46-47 -0.03634166979516351 2404 48-49 -0.07044183460442355 2404 50-51 0.06806828657870057 2404 52-53 0.04384619315230509 2404 54-55 0.01354518025328133 2404 56-57 -0.06744648828367161 2404 58-59 -0.02685903291144598 2404 60-61 0.061687904359509105 2404 62-63 0.07634140753771135 2404 64-65 0.08511541943788359 2404 66-67 0.10626529837146848 2404 68-69 0.11209306092654714 2404 70-71 0.0675563204127414 2404 72-73 0.14890527144395094 2404 74-75 0.17203159466102846 2404 76-77 0.15999755302723173 2404 78-79 -0.10941146764228904 2404 80-81 0.15663928147802153 2404 82-83 0.1412788309458861 2404 84-85 0.01738445407619338 2404 86-87 0.25732209645569526 2404 88-89 0.22546949924470283 2404 90-91 0.716033566958302 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 20.0 31 407.0 32 2882.0 33 5200.0 34 6913.0 35 8488.0 36 12479.0 37 19109.0 38 51568.0 39 307675.0 40 300251.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.376357545962502 11.965285985020664 32.251585697602046 29.406770771414788 2 27.29588232908078 17.35327151954771 29.22638587971349 26.124460271658016 3 26.787135394448836 19.50600362245362 21.967034273446295 31.739826709651254 4 26.46910734665563 20.91533891288294 20.46750173428514 32.14805200617629 5 26.679459350594133 21.42835164589254 20.553236959294647 31.338952044218676 6 27.40967731107481 23.589075122518853 23.460262492447466 25.540985073958872 7 25.576509946964443 23.46208069460917 23.22907109450176 27.732338263924632 8 24.61649920558552 24.81845950723924 23.954533757021057 26.61050753015418 9 24.675241121578985 24.799158591984245 24.330062434265 26.195537852171775 10-11 24.38300008951149 23.704810683196456 24.248383198693134 27.66380602859892 12-13 24.7463607984425 23.452500167834046 24.053346051424352 27.747792982299103 14-15 25.03447591021997 23.063195112672588 23.958939400720567 27.943389576386867 16-17 25.048532011546982 22.84703884798711 23.74641954035849 28.358009600107415 18-19 25.243429297111014 23.010956765949828 23.70984570456732 28.035768232371833 20-21 25.270912122093677 23.123405576565894 23.62774688388122 27.977935417459214 22-23 24.96496464296104 22.67689708416318 23.934603464094703 28.423534808781074 24-25 25.061679011793135 22.92193479087878 23.951736522926133 28.064649674401952 26-27 25.265877100722804 23.020537292724956 23.860616622283885 27.852968984268355 28-29 25.208114216662565 22.72472978718643 23.976072459551993 28.091083536599008 30-31 25.417034387941634 22.852519409389515 23.91295530420748 27.817490898461372 32-33 25.465793642563145 22.89914569036729 23.90786758737095 27.727193079698615 34-35 25.253324889170358 22.573289613087663 24.150207454144052 28.023178043597923 36-37 25.402994187410076 22.87587479132272 24.140434141026244 27.580696880240964 38-39 25.544276145654305 22.886342098077325 24.10106494048862 27.468316815779755 40-41 25.361040423546587 22.604801397211478 24.24780610993431 27.786352069307625 42-43 25.48474408573066 22.888498183099443 24.25927046371004 27.36748726745986 44-45 25.56391403088495 22.9128316801416 24.168930311198835 27.354323977774612 46-47 25.492340146004494 22.634820773801682 24.23107217836812 27.641766901825708 48-49 25.582215150368526 22.856533002163598 24.174851582704573 27.386400264763306 50-51 25.704917499834544 22.760548073718954 24.24683984483397 27.287694581612527 52-53 25.511429892637423 22.564613575133702 24.289275805219347 27.63468072700953 54-55 25.729789575118062 22.83619116611879 24.27929990500468 27.154719353758466 56-57 25.833139128408295 22.79547532402345 24.20659818702233 27.164787360545922 58-59 25.761759672637357 22.60298583867808 24.224704131575102 27.410550357109457 60-61 25.92838412743317 22.864598634126526 24.072885271705818 27.134131966734486 62-63 25.923492289365957 22.86221638149448 24.023800069739366 27.1904912594002 64-65 25.966929293612417 22.602373786900213 23.980207170955488 27.45048974853188 66-67 26.060633799414667 22.825780171328752 23.936442067844965 27.17714396141162 68-69 26.30435784573549 22.755110271241904 23.704860230912818 27.23567165210979 70-71 26.118989512565133 22.6485719939318 23.77151902908779 27.46091946441528 72-73 26.36210162076469 22.70061393179298 23.68801137164786 27.249273075794473 74-75 26.224506182752528 22.828899238085043 23.51364131113609 27.43295326802634 76-77 26.358322690732194 22.41464280604323 23.658899775842972 27.568134727381604 78-79 26.48423778572619 22.813866133353848 23.34397394971988 27.35792213120008 80-81 26.878114371589025 22.686071343826622 22.841097840702364 27.59471644388199 82-83 27.41515410528433 21.948815928402635 22.58233194227965 28.053698024033384 84-85 27.81006969672506 21.710933505556902 22.187239310029334 28.291757487688706 86-87 28.462803521331143 21.11637805939828 21.605881783883138 28.814936635387443 88-89 27.938834023574387 19.894870978018478 22.051608792609112 30.114686205798023 90-91 23.988755270966735 21.64610338903639 25.222551928783382 29.142589411213493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 1.0 8 2.0 9 3.5 10 4.0 11 9.5 12 16.0 13 17.5 14 21.0 15 34.5 16 56.0 17 77.5 18 108.5 19 157.0 20 229.0 21 341.0 22 476.0 23 643.5 24 891.5 25 1149.5 26 1473.0 27 1944.5 28 2501.0 29 3111.0 30 3751.0 31 4514.5 32 5403.5 33 6294.0 34 7352.5 35 8762.0 36 10543.5 37 12408.5 38 14348.5 39 16605.0 40 19172.0 41 22071.0 42 24900.0 43 27930.0 44 31414.0 45 35091.0 46 38814.0 47 42486.5 48 45866.5 49 48984.5 50 52123.0 51 54671.5 52 56237.0 53 57013.0 54 57095.5 55 56554.0 56 55328.0 57 53494.5 58 51418.0 59 49053.0 60 46464.0 61 43495.5 62 40377.5 63 36986.5 64 33384.5 65 30162.0 66 26759.5 67 23596.0 68 20673.5 69 17619.5 70 14936.5 71 12596.0 72 10317.0 73 8098.0 74 6267.5 75 4994.0 76 3883.5 77 2812.5 78 2057.5 79 1496.5 80 1025.0 81 707.5 82 516.0 83 367.0 84 257.0 85 183.0 86 111.5 87 66.0 88 44.5 89 26.5 90 15.5 91 11.5 92 8.5 93 7.0 94 6.0 95 3.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.790319332244276E-4 2 0.0068532235325709946 3 9.790319332244276E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.8806406544815567E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001005570862578686 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10339.0 32-33 22708.0 34-35 25099.0 36-37 26955.0 38-39 29150.0 40-41 30930.0 42-43 31644.0 44-45 32888.0 46-47 33284.0 48-49 33010.0 50-51 32986.0 52-53 32351.0 54-55 31274.0 56-57 30264.0 58-59 29345.0 60-61 27900.0 62-63 26640.0 64-65 25663.0 66-67 23310.0 68-69 22295.0 70-71 20755.0 72-73 19429.0 74-75 18155.0 76-77 16743.0 78-79 15089.0 80-81 13769.0 82-83 12926.0 84-85 11558.0 86-87 10445.0 88-89 9430.0 90-91 8658.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.7525160696976 #Duplication Level Percentage of deduplicated Percentage of total 1 99.7646593562345 99.5177578562069 2 0.22466157335635406 0.44821114412946517 3 0.008865747541038667 0.026531418721720247 4 0.0015484270933371416 0.006178379940434736 5 2.6489577477100746E-4 0.001321201001481996 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0018182021617025085 0.0 0.0 0.0 1.398617047463468E-4 2 0.0018182021617025085 0.0 0.0 0.0 1.398617047463468E-4 3 0.0018182021617025085 0.0 0.0 0.0 1.398617047463468E-4 4 0.0018182021617025085 0.0 0.0 0.0 1.398617047463468E-4 5 0.0018182021617025085 1.398617047463468E-4 0.0 0.0 1.398617047463468E-4 6 0.0018182021617025085 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 7 0.002377648980687896 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 8 0.005174883075614832 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 9 0.005174883075614832 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 10-11 0.005174883075614832 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 12-13 0.005174883075614832 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 14-15 0.005174883075614832 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 16-17 0.005314744780361179 1.398617047463468E-4 0.0 1.398617047463468E-4 1.398617047463468E-4 18-19 0.005524537337480699 1.398617047463468E-4 0.0 2.097925571195202E-4 1.398617047463468E-4 20-21 0.005944122451719739 1.398617047463468E-4 0.0 2.797234094926936E-4 2.797234094926936E-4 22-23 0.0064336384183319535 2.797234094926936E-4 0.0 2.797234094926936E-4 3.49654261865867E-4 24-25 0.006713361827824647 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 26-27 0.007272808646810034 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 28-29 0.007622462908675902 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 30-31 0.00839170228478081 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 32-33 0.008951149103766196 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 34-35 0.009580526775124757 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 36-37 0.00986025018461745 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 38-39 0.010349766151229664 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 40-41 0.010909212970215052 2.797234094926936E-4 0.0 2.797234094926936E-4 4.195851142390404E-4 42-43 0.01125886723208092 2.797234094926936E-4 0.0 2.797234094926936E-4 5.594468189853872E-4 44-45 0.011538590641573612 2.797234094926936E-4 0.0 2.797234094926936E-4 5.594468189853872E-4 46-47 0.011678452346319958 4.195851142390404E-4 0.0 2.797234094926936E-4 5.594468189853872E-4 48-49 0.012028106608185827 4.195851142390404E-4 0.0 2.797234094926936E-4 5.594468189853872E-4 50-51 0.012167968312932173 4.195851142390404E-4 0.0 2.797234094926936E-4 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