##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-18C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 375172 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.210506114528805 40.0 40.0 40.0 40.0 40.0 2 39.09307197765292 40.0 40.0 40.0 40.0 40.0 3 39.09268282281194 40.0 40.0 40.0 40.0 40.0 4 39.012218395829116 40.0 40.0 40.0 40.0 40.0 5 39.15453178808653 40.0 40.0 40.0 40.0 40.0 6 39.202232575991815 40.0 40.0 40.0 40.0 40.0 7 39.251372703719895 40.0 40.0 40.0 40.0 40.0 8 39.27819773330632 40.0 40.0 40.0 40.0 40.0 9 39.30959666499632 40.0 40.0 40.0 40.0 40.0 10-11 39.296200142867804 40.0 40.0 40.0 40.0 40.0 12-13 39.30422312965786 40.0 40.0 40.0 40.0 40.0 14-15 39.3170332540808 40.0 40.0 40.0 40.0 40.0 16-17 39.44838634013199 40.0 40.0 40.0 40.0 40.0 18-19 39.46379793801243 40.0 40.0 40.0 40.0 40.0 20-21 39.471426439073284 40.0 40.0 40.0 40.0 40.0 22-23 39.47485953109507 40.0 40.0 40.0 40.0 40.0 24-25 39.475739127653455 40.0 40.0 40.0 40.0 40.0 26-27 39.46354205537727 40.0 40.0 40.0 40.0 40.0 28-29 39.462156024436794 40.0 40.0 40.0 40.0 40.0 30-31 39.45088813367961 40.0 40.0 40.0 40.0 40.0 32-33 39.45189900651312 40.0 40.0 40.0 40.0 40.0 34-35 39.449240852074155 40.0 40.0 40.0 40.0 40.0 36-37 39.44232083246041 40.0 40.0 40.0 40.0 40.0 38-39 39.44075899505363 40.0 40.0 40.0 40.0 40.0 40-41 39.421926808675885 40.0 40.0 40.0 40.0 40.0 42-43 39.4192178036334 40.0 40.0 40.0 40.0 40.0 44-45 39.40590426959267 40.0 40.0 40.0 40.0 40.0 46-47 39.406222506472645 40.0 40.0 40.0 40.0 40.0 48-49 39.400551354051366 40.0 40.0 40.0 40.0 40.0 50-51 39.39160873432097 40.0 40.0 40.0 40.0 40.0 52-53 39.38431284322945 40.0 40.0 40.0 40.0 40.0 54-55 39.35178413121255 40.0 40.0 40.0 40.0 40.0 56-57 39.35896281193462 40.0 40.0 40.0 40.0 40.0 58-59 39.35426112953729 40.0 40.0 40.0 40.0 40.0 60-61 39.31678777871004 40.0 40.0 40.0 40.0 40.0 62-63 39.29741864735681 40.0 40.0 40.0 40.0 40.0 64-65 39.29016706855133 40.0 40.0 40.0 40.0 40.0 66-67 39.284378356774305 40.0 40.0 40.0 40.0 40.0 68-69 39.27957128276155 40.0 40.0 40.0 40.0 40.0 70-71 39.27745970350763 40.0 40.0 40.0 40.0 40.0 72-73 39.260749279207516 40.0 40.0 40.0 40.0 40.0 74-75 39.25452055512705 40.0 40.0 40.0 40.0 40.0 76-77 39.25128092428345 40.0 40.0 40.0 40.0 40.0 78-79 39.247146208396806 40.0 40.0 40.0 40.0 40.0 80-81 39.26735716154802 40.0 40.0 40.0 40.0 40.0 82-83 39.22443172609667 40.0 40.0 40.0 40.0 40.0 84-85 39.22898774241347 40.0 40.0 40.0 40.0 40.0 86-87 39.21341707713763 40.0 40.0 40.0 40.0 40.0 88-89 39.23268754879584 40.0 40.0 40.0 40.0 40.0 90-91 39.2119841718485 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04936667213902268 1101 2 0.09171982198768802 1101 3 0.20128621598458096 1101 4 -0.252432099491287 1101 5 0.07813550003914571 1101 6 -0.2586735114044245 1101 7 -0.13221737562795965 1101 8 -0.1100831890105951 1101 9 -0.1035517820765719 1101 10-11 -0.15129178546800404 1101 12-13 -0.09892736399428514 1101 14-15 -0.10504545827312484 1101 16-17 -0.07625756947345508 1101 18-19 -0.1080599194428018 1101 20-21 0.04363718945553785 1101 22-23 -0.06637442211577849 1101 24-25 0.01022151672077598 1101 26-27 -0.0042042407240856505 1101 28-29 -0.2251356919609293 1101 30-31 -0.06640111712091823 1101 32-33 -0.12354571608714338 1101 34-35 -0.014490743887620283 1101 36-37 0.006941383997137507 1101 38-39 -0.05369678575549841 1101 40-41 -0.10347364424030303 1101 42-43 -0.01961035056441318 1101 44-45 -0.027435592417106136 1101 46-47 0.013447479526163875 1101 48-49 0.0921445073157372 1101 50-51 -0.05670782554507525 1101 52-53 -0.3648499966810803 1101 54-55 -6.39255940754424E-4 1101 56-57 -0.20759414008091426 1101 58-59 0.017101672534046486 1101 60-61 -0.08347838392764828 1101 62-63 -0.40679599259907917 1101 64-65 -0.05643255185518825 1101 66-67 0.269115599519111 1101 68-69 0.1543011411690145 1101 70-71 0.011831793317789163 1101 72-73 0.2706058987551714 1101 74-75 0.13031689729702123 1101 76-77 0.1570539797207644 1101 78-79 0.4881793501576084 1101 80-81 0.3965481573484695 1101 82-83 -0.4756008369034248 1101 84-85 0.4964601121114427 1101 86-87 -0.8846994443928224 1101 88-89 -0.18802024924292482 1101 90-91 0.864840367965364 1102 1 0.4808047992293183 1102 2 0.31872651095090276 1102 3 0.2035158703881521 1102 4 -0.12422697128615567 1102 5 0.04608421798786111 1102 6 -0.20153861731300537 1102 7 0.10245375034751447 1102 8 0.21265928590579364 1102 9 0.09098556463468555 1102 10-11 -0.2271000351892951 1102 12-13 -0.14867652787388153 1102 14-15 -0.23784924868560609 1102 16-17 -0.023721337589392988 1102 18-19 -0.09036203761449002 1102 20-21 -0.003464259819821791 1102 22-23 -0.22105669636327008 1102 24-25 -0.10265473745982945 1102 26-27 -0.176723750200118 1102 28-29 -0.03868084246260395 1102 30-31 -0.22164080496930438 1102 32-33 -0.026067740091356484 1102 34-35 0.0061673565917246265 1102 36-37 0.15288596523856057 1102 38-39 0.10976041453674412 1102 40-41 -0.09197214779553775 1102 42-43 0.09014213797780002 1102 44-45 -0.03306716729064618 1102 46-47 0.21028291215783668 1102 48-49 -0.008875936394055373 1102 50-51 0.16009875981928445 1102 52-53 0.13165222383054953 1102 54-55 0.03062168170009727 1102 56-57 0.14827041960675302 1102 58-59 0.17120650269034599 1102 60-61 0.19791403657524853 1102 62-63 0.10863573719594655 1102 64-65 -0.07572912770272211 1102 66-67 0.24098872322178266 1102 68-69 0.11920840840006264 1102 70-71 -0.03622334564644092 1102 72-73 -0.020977501944436483 1102 74-75 0.2608812447529232 1102 76-77 0.06454417475435292 1102 78-79 0.17845465219761536 1102 80-81 -0.4400478839797657 1102 82-83 0.18468624443627846 1102 84-85 -0.5405769249255883 1102 86-87 0.4597821609583477 1102 88-89 -0.07690913813181055 1102 90-91 -1.8018262987013074 1103 1 0.2784530108902743 1103 2 0.20426056288622618 1103 3 0.2650869956535402 1103 4 -0.14905140375390147 1103 5 0.019340791562903803 1103 6 0.07875698941572296 1103 7 -0.09736659915436263 1103 8 0.10119431775292753 1103 9 0.005814896661469504 1103 10-11 0.10696823199999272 1103 12-13 0.04785703410554021 1103 14-15 -0.11080776575240492 1103 16-17 0.011902539594991879 1103 18-19 -0.044898212692068284 1103 20-21 -0.12427921980675194 1103 22-23 -0.1557700721594486 1103 24-25 0.028775933779762397 1103 26-27 -0.031982610236049425 1103 28-29 -0.015775403952069667 1103 30-31 -0.02855734914699326 1103 32-33 -0.15371026044209657 1103 34-35 -0.11212301456845353 1103 36-37 -0.18589946833946414 1103 38-39 -0.2974593335284581 1103 40-41 -0.02043069693190347 1103 42-43 -0.1707779896399373 1103 44-45 0.08948186851993256 1103 46-47 -0.009393074078850816 1103 48-49 -0.004466882494043034 1103 50-51 -0.13831312755483083 1103 52-53 -0.044281500551441866 1103 54-55 -0.0897485593357743 1103 56-57 0.2151279558542285 1103 58-59 -0.020136471663356303 1103 60-61 0.11841989281010967 1103 62-63 -0.5640674277728053 1103 64-65 -0.37004259904725245 1103 66-67 -0.37585742324704796 1103 68-69 -0.48685324700502974 1103 70-71 -0.2239939084170004 1103 72-73 0.12276176909625036 1103 74-75 -0.7517458796754397 1103 76-77 -0.15361064440075012 1103 78-79 -0.38219852385961417 1103 80-81 -0.14138687766526914 1103 82-83 -0.4511680973004104 1103 84-85 -0.8178878492953459 1103 86-87 -0.6685822282756035 1103 88-89 -0.3748334360561145 1103 90-91 0.864840367965364 1104 1 0.12316392598666681 1104 2 -0.061788405039493455 1104 3 -0.10455743322749811 1104 4 -0.335184414041521 1104 5 -0.10954309179992094 1104 6 -0.050997603101855304 1104 7 -0.02417024477573193 1104 8 0.017265526619020477 1104 9 0.047571079169685504 1104 10-11 -0.24123498394533982 1104 12-13 0.07113430765017625 1104 14-15 -0.10065737913861028 1104 16-17 -0.26583948442522143 1104 18-19 -0.13010467486827082 1104 20-21 -0.1727502734594708 1104 22-23 0.002940331868529711 1104 24-25 -0.12777189370625308 1104 26-27 -0.1863915217549561 1104 28-29 -0.2686785564870604 1104 30-31 -0.3020404696928978 1104 32-33 -0.05283757585915083 1104 34-35 -0.19734078948462752 1104 36-37 -0.18080126720590073 1104 38-39 0.036512864471355044 1104 40-41 -0.2918334518265411 1104 42-43 -0.09758431579555094 1104 44-45 -0.2633512331395167 1104 46-47 -0.20271619131294472 1104 48-49 -0.318101531547903 1104 50-51 -0.1514066760174657 1104 52-53 -0.0058595135090016015 1104 54-55 -0.2347210481064792 1104 56-57 -0.23391119266494798 1104 58-59 -0.05871711754441833 1104 60-61 -0.5363481226817299 1104 62-63 -0.6337860286149848 1104 64-65 -0.8062466647740081 1104 66-67 -0.9525260487065594 1104 68-69 -1.1106861160406254 1104 70-71 -1.3003544526346928 1104 72-73 -1.1039566521800666 1104 74-75 -0.9046791997246615 1104 76-77 -0.6414390065148226 1104 78-79 -0.8613960744178257 1104 80-81 -0.9284037893057899 1104 82-83 -0.6316402861759656 1104 84-85 -1.5179524905364516 1104 86-87 -0.6472412504128897 1104 88-89 -0.7356392968619758 1104 90-91 -4.690715187590193 1201 1 0.2867952689366078 1201 2 0.2989000984253778 1201 3 0.038324129975173093 1201 4 0.32936957965552693 1201 5 0.0332069772345136 1201 6 -0.1744793933511275 1201 7 0.1642871971367157 1201 8 -0.048402660996863744 1201 9 -0.07136977913658171 1201 10-11 0.11394829700947895 1201 12-13 0.12892161144991832 1201 14-15 0.023297790116302508 1201 16-17 -0.026642826130355957 1201 18-19 -0.1870547991302587 1201 20-21 -0.00828613411071899 1201 22-23 -0.04922778780869663 1201 24-25 -0.025561476738246824 1201 26-27 -0.10428217997093014 1201 28-29 -0.10329395796067331 1201 30-31 -0.2216975329021409 1201 32-33 -0.047551184549419645 1201 34-35 -0.07707541817865149 1201 36-37 -0.06723247487393991 1201 38-39 -0.15021792046697868 1201 40-41 -0.08617121587878529 1201 42-43 -0.2684061670894877 1201 44-45 -0.10280519817433031 1201 46-47 -0.10286573320643555 1201 48-49 -0.11850233635990293 1201 50-51 -0.06300740977889774 1201 52-53 0.010717580611228072 1201 54-55 0.007722830448642526 1201 56-57 0.028221112901505307 1201 58-59 -0.4786503763379031 1201 60-61 -0.023791866697187913 1201 62-63 0.039695067719954125 1201 64-65 0.07052275404748087 1201 66-67 -0.12232101557182062 1201 68-69 -0.3601503939511872 1201 70-71 -0.6715016000182388 1201 72-73 -0.7880062077497172 1201 74-75 -0.5963501612910633 1201 76-77 -0.5005564913465932 1201 78-79 -1.177609702339197 1201 80-81 -0.34606984946258024 1201 82-83 -0.6985362241520363 1201 84-85 -0.5498361841848549 1201 86-87 -0.39751995721333344 1201 88-89 -1.121353582576262 1201 90-91 0.06484036796536685 1202 1 0.06802340586540367 1202 2 0.21789166618401623 1202 3 -0.05630844768859333 1202 4 0.24602090381608832 1202 5 0.3425119003378754 1202 6 0.08365111205147713 1202 7 -0.023848749741389952 1202 8 0.1578742651013556 1202 9 0.08118791891471488 1202 10-11 0.030910562627127547 1202 12-13 0.049672453242088466 1202 14-15 0.03082584644579356 1202 16-17 -0.12702782075024288 1202 18-19 -0.0628050476788573 1202 20-21 0.05424582944367273 1202 22-23 0.0712456614730641 1202 24-25 0.14361113259244718 1202 26-27 0.0039459037705071864 1202 28-29 0.008235833094850875 1202 30-31 0.10702486592937532 1202 32-33 0.15581696461381966 1202 34-35 0.08770301183322005 1202 36-37 0.07487348162704421 1202 38-39 0.09668062679443068 1202 40-41 0.057166251220330366 1202 42-43 -0.01546783028302201 1202 44-45 0.10762806624505572 1202 46-47 0.03881323180998919 1202 48-49 0.20021402866360205 1202 50-51 0.17605956712628057 1202 52-53 0.21912216036395904 1202 54-55 0.25668100963714835 1202 56-57 -0.1363038524682878 1202 58-59 0.22272370178036027 1202 60-61 -0.06291664319140722 1202 62-63 0.09764224127784615 1202 64-65 -0.05648013052413603 1202 66-67 0.07738552658003783 1202 68-69 0.43615692117382565 1202 70-71 0.2859608855844016 1202 72-73 -0.15896199185271342 1202 74-75 0.5475157053004267 1202 76-77 0.049967386763491106 1202 78-79 -0.021377759399506147 1202 80-81 0.4997061289449789 1202 82-83 0.3917255819104071 1202 84-85 0.1981618138131438 1202 86-87 0.21501565532227573 1202 88-89 0.8119797507570752 1202 90-91 -1.135159632034636 1203 1 0.04400279526313966 1203 2 0.13658216276177626 1203 3 0.25688538644423176 1203 4 0.126234010518111 1203 5 0.05496464104739118 1203 6 0.028752236112175922 1203 7 0.025095784929852982 1203 8 -0.07431493390424748 1203 9 0.0461920496919177 1203 10-11 0.01062741149338109 1203 12-13 0.017811834509998903 1203 14-15 0.045519745504279285 1203 16-17 0.15674434101060797 1203 18-19 0.1675398863322073 1203 20-21 0.02926544828302724 1203 22-23 0.11917396849425899 1203 24-25 0.10319538256939609 1203 26-27 -0.01952895494562057 1203 28-29 0.09614328863018784 1203 30-31 0.07414425574113181 1203 32-33 0.02886155769690646 1203 34-35 0.08196765017824248 1203 36-37 0.07818449906650216 1203 38-39 0.18025446788068678 1203 40-41 0.14890689801934087 1203 42-43 0.11819275314756084 1203 44-45 0.20275211125884596 1203 46-47 -0.010149529110954347 1203 48-49 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-0.3042254638398987 1204 16-17 -0.03843444262614071 1204 18-19 -0.06284535570546979 1204 20-21 0.005469452878756442 1204 22-23 -0.0073843053461004615 1204 24-25 -0.17873948494484182 1204 26-27 -0.15513888416346333 1204 28-29 -0.018484724181945467 1204 30-31 -0.08125026200281127 1204 32-33 -0.05595421461566019 1204 34-35 -0.07757735659291143 1204 36-37 -0.14635058261603007 1204 38-39 -0.0936925726164759 1204 40-41 -0.07172476002889283 1204 42-43 -0.13932660002252106 1204 44-45 -0.22110431706970957 1204 46-47 -0.16754655379352812 1204 48-49 -0.3483418084408356 1204 50-51 -0.03490462895522484 1204 52-53 0.007406675384913797 1204 54-55 -0.07787331938713749 1204 56-57 -0.2570937318480091 1204 58-59 -0.1053291103706897 1204 60-61 -0.1765188861369964 1204 62-63 0.08187957786696387 1204 64-65 -0.025761697654921534 1204 66-67 -0.08868268543904634 1204 68-69 0.3613902352810143 1204 70-71 -0.1843028164629814 1204 72-73 -0.4145961216430649 1204 74-75 0.0034037915290809906 1204 76-77 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0.004947425628948565 2401 64-65 0.17273415512816115 2401 66-67 0.1318726922806448 2401 68-69 -0.02776942182996578 2401 70-71 -0.007791226706601151 2401 72-73 0.12268572581273673 2401 74-75 0.31384567163039634 2401 76-77 0.35019706690895447 2401 78-79 0.2165041898748541 2401 80-81 -0.020004057280957 2401 82-83 -0.09345433557916749 2401 84-85 0.14262698649229577 2401 86-87 -0.45612801582139184 2401 88-89 -0.17849643971911178 2401 90-91 0.42039592352092114 2402 1 0.3294476817705103 2402 2 0.303145340708717 2402 3 0.2908296865155222 2402 4 0.19354000749777356 2402 5 0.15501906844813362 2402 6 0.143341079261603 2402 7 0.0814549751114555 2402 8 0.22781080105730922 2402 9 0.16860004055424582 2402 10-11 0.12172063566527669 2402 12-13 0.15444760787736556 2402 14-15 0.1539912554430316 2402 16-17 0.05767113998952311 2402 18-19 0.15916879878074752 2402 20-21 0.12591624667933132 2402 22-23 0.09442866573225928 2402 24-25 0.09436434515247782 2402 26-27 0.04840564018351046 2402 28-29 0.08289673250479979 2402 30-31 0.07679663329864184 2402 32-33 0.12748570898722988 2402 34-35 0.06500372841749424 2402 36-37 0.059921235550973506 2402 38-39 0.12056054793338689 2402 40-41 0.06015368896621709 2402 42-43 0.1372018344190593 2402 44-45 -0.04147043726482735 2402 46-47 0.04822986223314274 2402 48-49 0.04104240382025637 2402 50-51 0.1256653491083881 2402 52-53 -0.004218463736584965 2402 54-55 0.1453354446533197 2402 56-57 0.13349180392566495 2402 58-59 0.1459424337549109 2402 60-61 -0.04095594830489091 2402 62-63 0.015532208708727069 2402 64-65 0.03884847455055507 2402 66-67 0.22342270697748035 2402 68-69 0.08769586616732994 2402 70-71 -0.03678075186422802 2402 72-73 0.1479417057705703 2402 74-75 0.23277204353958325 2402 76-77 0.21735227756197872 2402 78-79 0.1179157091659846 2402 80-81 -0.241096546640307 2402 82-83 -0.1604117947794066 2402 84-85 -0.567299850382696 2402 86-87 0.08141777172439646 2402 88-89 0.3199014655753416 2402 90-91 -0.02404852092352172 2403 1 -0.259145572979115 2403 2 -0.17475984140641998 2403 3 -0.06480505599729014 2403 4 0.05128998487596448 2403 5 0.10096428415825187 2403 6 0.14184434381188993 2403 7 0.13314026813080915 2403 8 0.09131933553358351 2403 9 -0.03118151559690574 2403 10-11 0.13998019640706616 2403 12-13 0.13141032076135417 2403 14-15 0.11213007306790246 2403 16-17 0.06302390641391753 2403 18-19 0.07996467867996415 2403 20-21 0.15340091801309796 2403 22-23 0.0882485145548415 2403 24-25 0.02476461737887803 2403 26-27 0.10098510008938177 2403 28-29 0.09295687465898084 2403 30-31 0.09519128766923757 2403 32-33 0.14849352156441142 2403 34-35 6.548258384952987E-5 2403 36-37 0.09353272368598908 2403 38-39 0.07644859284123129 2403 40-41 0.11078848147430875 2403 42-43 0.09144065306650617 2403 44-45 0.0512347084190381 2403 46-47 0.0038641438776423342 2403 48-49 0.008070911854503038 2403 50-51 -0.04331388581962159 2403 52-53 0.04663588261418994 2403 54-55 0.06918774245767167 2403 56-57 -0.04678010529615051 2403 58-59 -0.012266516639698466 2403 60-61 0.023807280763627148 2403 62-63 0.2710012477653194 2403 64-65 0.179770720628035 2403 66-67 0.11737928855637136 2403 68-69 0.11944205999949986 2403 70-71 0.24562805220243433 2403 72-73 0.06399817942230612 2403 74-75 0.22270726843765942 2403 76-77 0.06711293687559561 2403 78-79 0.06389880889592803 2403 80-81 0.2897278719078997 2403 82-83 0.5197289794790194 2403 84-85 0.42912004477138055 2403 86-87 0.25497666896750104 2403 88-89 -0.738020249242922 2403 90-91 0.864840367965364 2404 1 -0.3105626398763306 2404 2 -0.20151299449072013 2404 3 -0.4483930163619334 2404 4 -0.10539722868355739 2404 5 -0.0034518250515489513 2404 6 0.0541497911665445 2404 7 -0.08551051509509477 2404 8 0.06588353999626406 2404 9 0.09166422508235428 2404 10-11 0.08157941497234589 2404 12-13 0.1209521831648388 2404 14-15 0.057037970287268536 2404 16-17 0.11686934378569447 2404 18-19 0.10183768598561471 2404 20-21 0.11569000586958111 2404 22-23 0.07947265878591026 2404 24-25 0.1248384142392922 2404 26-27 0.1154889296449042 2404 28-29 0.06757887472107882 2404 30-31 0.095830382809865 2404 32-33 0.06357181829693559 2404 34-35 0.06611859344505433 2404 36-37 0.0531336234413331 2404 38-39 0.07439836333317373 2404 40-41 0.07323378521798674 2404 42-43 0.12058689359620445 2404 44-45 0.18814191587098605 2404 46-47 0.1664696099430074 2404 48-49 0.15356586790140625 2404 50-51 0.12953995020687614 2404 52-53 0.15671085137291385 2404 54-55 0.09883059653978421 2404 56-57 0.24871656836691614 2404 58-59 0.11835596549773442 2404 60-61 0.06396577704159512 2404 62-63 0.423979469987124 2404 64-65 0.3304907267988213 2404 66-67 0.2748295250679007 2404 68-69 0.24685731577571346 2404 70-71 0.2794127223741114 2404 72-73 0.5197062719467311 2404 74-75 0.5640866835735352 2404 76-77 0.3106358070313675 2404 78-79 0.32867524835388195 2404 80-81 0.15524066726639774 2404 82-83 0.37214987115224574 2404 84-85 0.16678238243371624 2404 86-87 0.352015027575888 2404 88-89 0.10542704420436877 2404 90-91 0.864840367965364 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 8.0 31 269.0 32 1677.0 33 3120.0 34 3818.0 35 4829.0 36 7046.0 37 10605.0 38 27218.0 39 153062.0 40 163520.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.742471332690066 12.729565045873025 32.26358465319352 29.264378968243392 2 27.152046019784454 17.256352910756696 29.161679679482223 26.429921389976624 3 26.91380928010918 19.322276953258275 22.191658137154555 31.572255629477997 4 26.39029565106138 20.359461793524037 20.53724691608116 32.71299563933343 5 26.742667363236063 20.719829838047616 20.35226509441003 32.18523770430629 6 27.57935027134221 23.289584510571153 22.88950134871472 26.241563869371916 7 25.79750087959656 22.967065772499016 22.86924397343085 28.366189374473578 8 25.057840137323677 24.30778416299724 23.094207456846462 27.540168242832618 9 24.964283048841597 24.447986523514547 23.668610663908822 26.919119763735033 10-11 24.70826714147111 23.44564626358044 23.441514825200173 28.404571769748276 12-13 25.071966991140066 23.2078886484066 23.23254400648236 28.487600353970976 14-15 25.349039907029308 22.929483010459204 23.01717612188543 28.70430096062606 16-17 25.445528984039324 22.625222564583712 22.86244709093429 29.06680136044268 18-19 25.55307965413197 22.91668887870097 22.878039939014638 28.65219152815242 20-21 25.528291023850393 23.071950998475366 22.851918586674913 28.54783939099933 22-23 25.209237363129443 22.585640719456677 23.205089932084483 29.000031985329393 24-25 25.27054257780431 22.847920420500465 23.284786711161814 28.596750290533407 26-27 25.40714658876462 22.999717462923673 23.25333447058949 28.339801477722222 28-29 25.45659057712196 22.684262151759725 23.355554252449544 28.50359301866877 30-31 25.673328988630473 22.839276646955575 23.389161432365903 28.098232932048045 32-33 25.722444051736996 22.91603072243721 23.355294227154943 28.00623099867085 34-35 25.591087246944227 22.57359451479019 23.601857488911097 28.23346074935448 36-37 25.682784323621327 22.781277576781974 23.69061146357961 27.845326636017088 38-39 25.683524177529925 22.951629213394796 23.622375400835722 27.74247120823956 40-41 25.67670018578534 22.63381959660421 23.6855479001258 28.003932317484647 42-43 25.65990194980925 22.96592053989797 23.80391383381932 27.57026367647346 44-45 25.847616616923574 22.879791859909222 23.739550617011183 27.53304090615602 46-47 25.79973767988325 22.76211998990129 23.824536988573268 27.613605341642188 48-49 25.885458010090296 22.935524826621663 23.746884304007434 27.432132859280607 50-51 26.037514568941077 23.00572095964797 23.65051018553743 27.30625428587352 52-53 25.909950131365804 22.62646775771426 23.830101990768604 27.633480120151326 54-55 25.90359334941279 23.073823386847163 23.80469886061682 27.21788440312323 56-57 26.072055585552672 23.006314482571316 23.773558063817333 27.148071868058675 58-59 25.961503919300487 22.771099918811924 23.818641644453706 27.448754517433883 60-61 25.967848032216146 23.04264467583308 23.83802082497714 27.15148646697364 62-63 26.34993674106158 22.96952314916953 23.483248762961328 27.19729134680756 64-65 26.185388685388684 22.691290191290193 23.686406186406188 27.436914936914935 66-67 26.14947517041049 23.080929069888555 23.61733152796584 27.152264231735117 68-69 26.360386601350456 22.834635244273798 23.569442605587184 27.235535548788558 70-71 26.125297844791262 23.10279571869661 23.302763539966747 27.469142896545385 72-73 26.31188892361266 23.274253314880127 23.40104468949507 27.012813072012143 74-75 26.595711148701895 23.019216827594903 23.11798018428822 27.267091839414988 76-77 26.32858413844091 22.778592283498114 23.283585830106638 27.609237747954335 78-79 26.55068309343742 22.82111490607465 23.18705781898024 27.44114418150769 80-81 26.587181044957475 22.763517618469013 22.911603888213854 27.73769744835966 82-83 27.00578486172472 22.070345871268522 22.795284239302887 28.128585027703874 84-85 27.273620757436774 22.369283187000395 22.205477656925698 28.15161839863714 86-87 27.81137205268832 21.305020390943607 21.9940936577134 28.889513898654666 88-89 27.72444512710237 20.328699792642656 22.471392366177714 29.47546271407726 90-91 24.012667660208646 20.864381520119224 25.838301043219076 29.284649776453055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 2.5 12 6.0 13 8.5 14 11.0 15 14.0 16 19.5 17 34.0 18 59.5 19 90.5 20 121.0 21 164.5 22 225.0 23 305.5 24 416.0 25 536.5 26 689.5 27 893.0 28 1145.5 29 1424.5 30 1711.5 31 2001.5 32 2382.5 33 2864.5 34 3474.5 35 4150.0 36 4839.5 37 5672.0 38 6653.0 39 7829.0 40 9185.5 41 10602.5 42 12094.5 43 13839.0 44 15734.0 45 17690.5 46 19756.5 47 21796.0 48 23649.0 49 25576.5 50 27453.0 51 28991.0 52 30109.0 53 30845.0 54 31319.0 55 31304.5 56 31125.0 57 30693.5 58 29649.0 59 28096.0 60 26407.0 61 24776.0 62 23301.5 63 21731.5 64 19702.0 65 17747.5 66 15787.5 67 13919.0 68 12240.0 69 10546.0 70 8958.0 71 7510.5 72 6186.0 73 4935.0 74 3819.5 75 2970.5 76 2306.0 77 1684.5 78 1253.0 79 917.5 80 632.5 81 450.5 82 314.5 83 212.5 84 156.0 85 108.5 86 70.0 87 44.5 88 28.5 89 18.5 90 11.0 91 7.5 92 5.0 93 2.5 94 1.0 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015992664697791947 2 0.007729787937266108 3 0.0010661776465194631 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 7.661543647814161E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6419.0 32-33 14283.0 34-35 15577.0 36-37 16537.0 38-39 17680.0 40-41 18672.0 42-43 18737.0 44-45 18963.0 46-47 18824.0 48-49 18524.0 50-51 17974.0 52-53 17549.0 54-55 16686.0 56-57 15862.0 58-59 14664.0 60-61 13847.0 62-63 13004.0 64-65 12118.0 66-67 11053.0 68-69 10538.0 70-71 9416.0 72-73 8671.0 74-75 7850.0 76-77 7291.0 78-79 6543.0 80-81 6052.0 82-83 5365.0 84-85 4710.0 86-87 4250.0 88-89 3914.0 90-91 3599.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.59920912558887 #Duplication Level Percentage of deduplicated Percentage of total 1 99.62035269275134 99.2210834101026 2 0.358772809666251 0.7146697619704805 3 0.018992630808237477 0.05674953023144239 4 0.00188186677417021 0.007497297695483043 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015992664697791945 0.0 0.0 0.0 0.0 2 0.0015992664697791945 0.0 0.0 0.0 0.0 3 0.0015992664697791945 0.0 0.0 0.0 0.0 4 0.0015992664697791945 0.0 0.0 0.0 0.0 5 0.0015992664697791945 0.0 0.0 0.0 0.0 6 0.0015992664697791945 0.0 0.0 0.0 0.0 7 0.0021323552930389263 0.0 0.0 0.0 0.0 8 0.003198532939558389 0.0 0.0 0.0 0.0 9 0.003198532939558389 0.0 0.0 0.0 0.0 10-11 0.003198532939558389 0.0 0.0 0.0 0.0 12-13 0.003198532939558389 0.0 0.0 0.0 0.0 14-15 0.003331805145373322 0.0 0.0 0.0 0.0 16-17 0.003465077351188255 0.0 2.665444116298658E-4 0.0 0.0 18-19 0.003598349557003188 0.0 2.665444116298658E-4 0.0 0.0 20-21 0.003731621762818121 0.0 2.665444116298658E-4 0.0 0.0 22-23 0.0038648939686330534 0.0 2.665444116298658E-4 0.0 0.0 24-25 0.004264710586077853 0.0 2.665444116298658E-4 0.0 0.0 26-27 0.004531254997707718 0.0 2.665444116298658E-4 0.0 0.0 28-29 0.004531254997707718 0.0 2.665444116298658E-4 0.0 0.0 30-31 0.005330888232597315 0.0 2.665444116298658E-4 0.0 0.0 32-33 0.0057307048500421145 0.0 2.665444116298658E-4 0.0 0.0 34-35 0.006130521467486913 0.0 2.665444116298658E-4 0.0 0.0 36-37 0.006397065879116778 0.0 2.665444116298658E-4 0.0 1.332722058149329E-4 38-39 0.006663610290746644 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 40-41 0.007729787937266107 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 42-43 0.007729787937266107 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 44-45 0.007729787937266107 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 46-47 0.007729787937266107 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 48-49 0.008129604554710906 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 50-51 0.008262876760525839 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 52-53 0.008262876760525839 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 54-55 0.008262876760525839 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 56-57 0.008262876760525839 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 58-59 0.008529421172155705 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 60-61 0.008529421172155705 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 62-63 0.008795965583785571 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 64-65 0.009329054407045302 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 66-67 0.009995415436119967 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 68-69 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 70-71 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 72-73 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 74-75 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 76-77 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 78-79 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 80 0.010128687641934898 0.0 2.665444116298658E-4 0.0 2.665444116298658E-4 >>END_MODULE