##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-18B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 795128 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.282943123622864 40.0 40.0 40.0 40.0 40.0 2 39.150033705265066 40.0 40.0 40.0 40.0 40.0 3 39.120596935336195 40.0 40.0 40.0 40.0 40.0 4 39.01803986276423 40.0 40.0 40.0 40.0 40.0 5 39.17516173496594 40.0 40.0 40.0 40.0 40.0 6 39.23890996166655 40.0 40.0 40.0 40.0 40.0 7 39.25416285176726 40.0 40.0 40.0 40.0 40.0 8 39.29387469690415 40.0 40.0 40.0 40.0 40.0 9 39.30583755068366 40.0 40.0 40.0 40.0 40.0 10-11 39.29742381100905 40.0 40.0 40.0 40.0 40.0 12-13 39.29764012838184 40.0 40.0 40.0 40.0 40.0 14-15 39.31044058315139 40.0 40.0 40.0 40.0 40.0 16-17 39.45020424384502 40.0 40.0 40.0 40.0 40.0 18-19 39.46722791802075 40.0 40.0 40.0 40.0 40.0 20-21 39.473478483967355 40.0 40.0 40.0 40.0 40.0 22-23 39.470246299966796 40.0 40.0 40.0 40.0 40.0 24-25 39.47801863347788 40.0 40.0 40.0 40.0 40.0 26-27 39.46812840196799 40.0 40.0 40.0 40.0 40.0 28-29 39.46348512440764 40.0 40.0 40.0 40.0 40.0 30-31 39.449232328734254 40.0 40.0 40.0 40.0 40.0 32-33 39.45337508101892 40.0 40.0 40.0 40.0 40.0 34-35 39.44621783168404 40.0 40.0 40.0 40.0 40.0 36-37 39.44861910449741 40.0 40.0 40.0 40.0 40.0 38-39 39.432768751230455 40.0 40.0 40.0 40.0 40.0 40-41 39.41905289104789 40.0 40.0 40.0 40.0 40.0 42-43 39.41505114195388 40.0 40.0 40.0 40.0 40.0 44-45 39.39433476804861 40.0 40.0 40.0 40.0 40.0 46-47 39.39556023861677 40.0 40.0 40.0 40.0 40.0 48-49 39.388982637146675 40.0 40.0 40.0 40.0 40.0 50-51 39.38944938650968 40.0 40.0 40.0 40.0 40.0 52-53 39.37635747067547 40.0 40.0 40.0 40.0 40.0 54-55 39.34919256384798 40.0 40.0 40.0 40.0 40.0 56-57 39.35492392394116 40.0 40.0 40.0 40.0 40.0 58-59 39.34172436292337 40.0 40.0 40.0 40.0 40.0 60-61 39.301527251665604 40.0 40.0 40.0 40.0 40.0 62-63 39.38583810597187 40.0 40.0 40.0 40.0 40.0 64-65 39.37520958157991 40.0 40.0 40.0 40.0 40.0 66-67 39.372244762188714 40.0 40.0 40.0 40.0 40.0 68-69 39.38004092697506 40.0 40.0 40.0 40.0 40.0 70-71 39.36321353074921 40.0 40.0 40.0 40.0 40.0 72-73 39.3662902208213 40.0 40.0 40.0 40.0 40.0 74-75 39.35580342053653 40.0 40.0 40.0 40.0 40.0 76-77 39.35609451825718 40.0 40.0 40.0 40.0 40.0 78-79 39.352118576157686 40.0 40.0 40.0 40.0 40.0 80-81 39.349477457423966 40.0 40.0 40.0 40.0 40.0 82-83 39.32293536097366 40.0 40.0 40.0 40.0 40.0 84-85 39.352414942943426 40.0 40.0 40.0 40.0 40.0 86-87 39.33553496658289 40.0 40.0 40.0 40.0 40.0 88-89 39.37548045065323 40.0 40.0 40.0 40.0 40.0 90-91 39.414125859807655 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17750281988951855 1101 2 0.11423855386065895 1101 3 0.2574382585520141 1101 4 0.1569952460002355 1101 5 0.023947418062547854 1101 6 -0.022862086195317488 1101 7 -0.021223508454902174 1101 8 0.04496698325090165 1101 9 0.08319313985280274 1101 10-11 0.016616188167951407 1101 12-13 0.11369586020153122 1101 14-15 0.00404166449911969 1101 16-17 -0.024308545179458463 1101 18-19 0.07600993254738597 1101 20-21 -0.02354631407689567 1101 22-23 -0.05775285861358981 1101 24-25 0.02896174955321129 1101 26-27 0.06067137127237032 1101 28-29 0.0349906707146701 1101 30-31 0.028468007205340484 1101 32-33 -0.02049802156419389 1101 34-35 -0.05287580974823669 1101 36-37 0.06181019208946026 1101 38-39 0.07666746171872063 1101 40-41 0.09493309385651116 1101 42-43 0.03394077450469268 1101 44-45 0.06410923493359633 1101 46-47 0.09464165598686236 1101 48-49 0.024558049173840857 1101 50-51 0.03135449001549517 1101 52-53 0.11840302482253406 1101 54-55 -0.02359632032280956 1101 56-57 0.14632518344915013 1101 58-59 0.14315447807996406 1101 60-61 0.10945882653498273 1101 62-63 -0.06128164189418328 1101 64-65 -0.04534524531883477 1101 66-67 0.045243256898238826 1101 68-69 -0.03646700714588036 1101 70-71 -0.15093741962325424 1101 72-73 0.0016046865091396967 1101 74-75 -0.010144641343458716 1101 76-77 0.06469264231381544 1101 78-79 -0.16663981896854096 1101 80-81 -0.081432916026273 1101 82-83 0.11074138324095628 1101 84-85 0.17634923147970483 1101 86-87 0.05097703784833385 1101 88-89 -0.5434412500392583 1101 90-91 0.3643941001306956 1102 1 0.31312482267267683 1102 2 0.1917447548342821 1102 3 0.2694495707324762 1102 4 0.07398386027723092 1102 5 0.030005604541607056 1102 6 0.13082368465948946 1102 7 0.19075953282538904 1102 8 0.10418974361777344 1102 9 0.1417139032053143 1102 10-11 0.035183292874393146 1102 12-13 -0.023193557599398673 1102 14-15 -0.008484445491880876 1102 16-17 0.09051476646996548 1102 18-19 0.03985708630765572 1102 20-21 -0.010162048552018632 1102 22-23 0.041664245089990004 1102 24-25 0.062232587642135684 1102 26-27 -0.025809885407348077 1102 28-29 0.019787421089304758 1102 30-31 -0.05743159480532967 1102 32-33 0.027986997277707815 1102 34-35 -0.03246904118490335 1102 36-37 -0.07332265536670945 1102 38-39 0.069925624359513 1102 40-41 -0.011806579858500754 1102 42-43 -0.0115082567461684 1102 44-45 0.005795660312315931 1102 46-47 -0.08330105857706371 1102 48-49 -0.06032624328150149 1102 50-51 -0.05181969078150672 1102 52-53 -0.127964250009299 1102 54-55 -0.1380666346375392 1102 56-57 0.04069420421286196 1102 58-59 -9.791422606966194E-4 1102 60-61 0.004863182619196493 1102 62-63 -0.14345025906398945 1102 64-65 -0.09221180389481276 1102 66-67 0.033686134598816864 1102 68-69 -0.12316182050047786 1102 70-71 0.15431056516550967 1102 72-73 -0.02266124794564206 1102 74-75 0.16799590794151698 1102 76-77 -0.22300345190369342 1102 78-79 -0.253786153669175 1102 80-81 0.02650644999692986 1102 82-83 -0.19590808748176158 1102 84-85 -0.48082311114155374 1102 86-87 -0.05623849093345967 1102 88-89 -0.32160752820553284 1102 90-91 0.3643941001306956 1103 1 0.35834856730973996 1103 2 0.2800805818233485 1103 3 0.10526476016377728 1103 4 0.13937836920010938 1103 5 0.23113836928997245 1103 6 0.15696128725729608 1103 7 0.022631756961771998 1103 8 -0.05998657121530471 1103 9 -0.007027547960724689 1103 10-11 0.009926886853747874 1103 12-13 0.04097378951276198 1103 14-15 0.1498024366203552 1103 16-17 0.04167844625624895 1103 18-19 -0.005053260082362954 1103 20-21 -0.023944963627698712 1103 22-23 -0.021691287285840133 1103 24-25 0.025043920953443433 1103 26-27 -0.03469170500511609 1103 28-29 -0.027990236070003505 1103 30-31 -0.0613769200268095 1103 32-33 0.07385046287575392 1103 34-35 0.03523337810255356 1103 36-37 -0.015473836669947616 1103 38-39 -0.05638471808889278 1103 40-41 -0.05235964437105167 1103 42-43 -0.03865110957400475 1103 44-45 -0.006287003337703823 1103 46-47 0.02879871842304027 1103 48-49 -0.013881201027679424 1103 50-51 0.023576361019884473 1103 52-53 -0.06609245301629585 1103 54-55 -0.03298421881392244 1103 56-57 0.08252542472530422 1103 58-59 -0.09725864606683388 1103 60-61 -0.04848573170274051 1103 62-63 0.09112167205367427 1103 64-65 0.07286249140250334 1103 66-67 0.03859128768004183 1103 68-69 -0.04180651323313356 1103 70-71 -0.1149209398414115 1103 72-73 0.02972744995577159 1103 74-75 -0.14317401864498436 1103 76-77 -0.13453788012279944 1103 78-79 0.010838504812419103 1103 80-81 -0.29898797439908265 1103 82-83 -0.12116800787288895 1103 84-85 0.20584985983509796 1103 86-87 0.35807730885104405 1103 88-89 0.39090218430418133 1103 90-91 0.3643941001306956 1104 1 0.24589053264863736 1104 2 0.04901262065830281 1104 3 0.013948182720717739 1104 4 0.026811451497543715 1104 5 0.05269721150029483 1104 6 0.04811454013815819 1104 7 0.053300480308109854 1104 8 -0.08005734519225882 1104 9 0.07632242028171987 1104 10-11 0.09992779421241949 1104 12-13 -0.05569620413572807 1104 14-15 0.07716837437553892 1104 16-17 -0.024132278723335787 1104 18-19 0.0768086399267105 1104 20-21 0.028373337796857356 1104 22-23 0.046877808077553595 1104 24-25 0.055921336795925924 1104 26-27 -0.05145061980413601 1104 28-29 0.05514196608950783 1104 30-31 -0.008922514800424608 1104 32-33 0.08043288489837153 1104 34-35 0.06483546436340504 1104 36-37 -0.06459467424186016 1104 38-39 -0.020520878868438786 1104 40-41 0.07052068558803626 1104 42-43 0.07853047525402701 1104 44-45 0.03773459469536533 1104 46-47 -0.024104188494085577 1104 48-49 -0.057783854073598206 1104 50-51 -0.1283347155911727 1104 52-53 -0.014128192734418121 1104 54-55 0.01269018861184179 1104 56-57 -0.06020697409132936 1104 58-59 0.10431118349118407 1104 60-61 0.11392548035360761 1104 62-63 -0.08470926726334937 1104 64-65 -0.08300347886731174 1104 66-67 -0.08503589387392907 1104 68-69 -0.0318904041434962 1104 70-71 0.08608034558984201 1104 72-73 -0.12911079848034035 1104 74-75 -0.10950919571916984 1104 76-77 0.007418107978303112 1104 78-79 0.20609568820652413 1104 80-81 -0.005657142997428366 1104 82-83 -0.21204722760603545 1104 84-85 0.2577515485040749 1104 86-87 0.43931037118166216 1104 88-89 0.3778302888793448 1104 90-91 -0.33125807378235095 1201 1 0.24321968324881738 1201 2 0.2028587745044561 1201 3 0.1296791806947013 1201 4 0.1465116889547886 1201 5 0.11413110470989096 1201 6 0.08890054541014791 1201 7 0.04931381277004476 1201 8 0.11943724121821475 1201 9 -0.03044620050559388 1201 10-11 0.058418524665768246 1201 12-13 0.015074769438982116 1201 14-15 -0.025851036120506876 1201 16-17 -0.08711509030427322 1201 18-19 -0.017228786821000597 1201 20-21 -0.043194969285465845 1201 22-23 0.011657887810905265 1201 24-25 -0.03342576470070924 1201 26-27 -0.02955444744068103 1201 28-29 -0.04907562317480796 1201 30-31 0.048363501364100614 1201 32-33 -0.04869492066520564 1201 34-35 0.057332391248742454 1201 36-37 -0.011494327394515835 1201 38-39 -0.005605652560049634 1201 40-41 -0.04180674180898336 1201 42-43 -0.029642028236779083 1201 44-45 -0.057810629759842413 1201 46-47 -0.050580569400167974 1201 48-49 0.1350482208098498 1201 50-51 0.02417478501525494 1201 52-53 0.10869455292294106 1201 54-55 0.00858289696472525 1201 56-57 -0.0967305802765992 1201 58-59 0.0718067183487534 1201 60-61 -0.008170784953492216 1201 62-63 -0.20979962828751297 1201 64-65 -0.11401710137329246 1201 66-67 -0.01787668977252821 1201 68-69 -0.04745036328895935 1201 70-71 0.09917540210381759 1201 72-73 9.852408679051905E-4 1201 74-75 -0.07542470002505297 1201 76-77 -0.32325125224581797 1201 78-79 0.331274819586028 1201 80-81 -0.12359075818410759 1201 82-83 0.029053513577174783 1201 84-85 -0.18395974059397702 1201 86-87 0.1415830984543902 1201 88-89 0.32741012081211807 1201 90-91 0.3643941001306956 1202 1 0.11256727176499481 1202 2 0.22637982620102548 1202 3 0.06993661379278393 1202 4 0.08952971788505693 1202 5 -0.0697607540087617 1202 6 0.004433203667979058 1202 7 -0.07360806904329564 1202 8 0.008432003192190507 1202 9 0.022237682201385667 1202 10-11 -0.04288371428081206 1202 12-13 -0.08109172765130523 1202 14-15 0.07306529004755902 1202 16-17 0.0435394745304265 1202 18-19 0.021126699109544234 1202 20-21 0.004553433197344248 1202 22-23 0.0026590344702484003 1202 24-25 -0.04396211057192545 1202 26-27 0.058866446522941374 1202 28-29 0.002609104722388622 1202 30-31 0.05684770384367965 1202 32-33 0.05637770061441927 1202 34-35 -0.0973045892292177 1202 36-37 0.01869870382879668 1202 38-39 0.012381635201883512 1202 40-41 0.11356289636310635 1202 42-43 -2.586525330485756E-4 1202 44-45 0.07739286175018378 1202 46-47 -0.010166785371389153 1202 48-49 -0.03810142134965844 1202 50-51 -0.032984319238842374 1202 52-53 0.08968568779653197 1202 54-55 -0.10895670675989777 1202 56-57 0.0719892531482742 1202 58-59 -0.05838574598710977 1202 60-61 -0.14082273214677343 1202 62-63 -0.11572196167333004 1202 64-65 -0.23068738029465408 1202 66-67 -0.22388151579405502 1202 68-69 0.043419379690405435 1202 70-71 -0.131826474683038 1202 72-73 -0.25965210141380624 1202 74-75 -0.29870358570235567 1202 76-77 -0.14673314945027727 1202 78-79 -0.36308041354085674 1202 80-81 0.05376823104599282 1202 82-83 -0.2439959447430482 1202 84-85 0.11483590820167677 1202 86-87 -0.4871234708441605 1202 88-89 0.32741012081211807 1202 90-91 -0.16893923320263582 1203 1 0.22703677939468037 1203 2 0.2681907028500774 1203 3 0.19953261493169805 1203 4 0.0850626357249169 1203 5 0.01514333448924532 1203 6 0.16083591597565317 1203 7 -0.007664825030509803 1203 8 0.07846269065462508 1203 9 0.05340602033292896 1203 10-11 0.11619545093584804 1203 12-13 -0.08946908852953328 1203 14-15 0.007868668832081482 1203 16-17 0.034780365837754346 1203 18-19 0.002332473836155202 1203 20-21 -0.008676975863366465 1203 22-23 0.0955228812649409 1203 24-25 0.02281844770789121 1203 26-27 0.0360712510617347 1203 28-29 0.03712037261022516 1203 30-31 0.05536289466486721 1203 32-33 0.06969511960936359 1203 34-35 0.032906336888622434 1203 36-37 0.006306349489001661 1203 38-39 0.007643168063637518 1203 40-41 -0.08827222831992998 1203 42-43 -0.0759687762517558 1203 44-45 -0.06270737056603082 1203 46-47 0.039038221044741306 1203 48-49 -0.03313809174730409 1203 50-51 0.0535873030630043 1203 52-53 0.005781084867351183 1203 54-55 0.014930337914435654 1203 56-57 -0.043898943376376565 1203 58-59 -0.04138774931706024 1203 60-61 0.0642191231289857 1203 62-63 -0.0163040123360787 1203 64-65 -0.15517356446950714 1203 66-67 -0.03232659338885213 1203 68-69 0.21594748665665264 1203 70-71 -0.26757961043050926 1203 72-73 0.12792951768619787 1203 74-75 0.02631192868095411 1203 76-77 0.40795563459563766 1203 78-79 0.25119256527923284 1203 80-81 0.07876213339579863 1203 82-83 -0.173747545565071 1203 84-85 -0.42158670329024517 1203 86-87 0.3397953800000053 1203 88-89 -0.7157894129534554 1203 90-91 0.044394100130695335 1204 1 0.26120229534382844 1204 2 0.12109112199639327 1204 3 0.16138663470367476 1204 4 0.09685738845303149 1204 5 -0.06816616982645485 1204 6 -0.028997851168000466 1204 7 -0.013447333309571263 1204 8 -0.03729012300245671 1204 9 -0.10529279310419071 1204 10-11 -0.043498885904625695 1204 12-13 -0.04695567706961157 1204 14-15 -0.05143259105084752 1204 16-17 -0.05125175649268243 1204 18-19 -0.08255341164606733 1204 20-21 -0.08549195270644816 1204 22-23 -0.0931548730232663 1204 24-25 -0.05917699830207823 1204 26-27 -0.14487938417506996 1204 28-29 -0.17498884168391982 1204 30-31 -0.14508598700064113 1204 32-33 -0.1530277997188918 1204 34-35 -0.005933286297711504 1204 36-37 -0.11733104848060805 1204 38-39 0.021393233653583366 1204 40-41 -0.001751111547591222 1204 42-43 -0.026896669515238614 1204 44-45 -0.034370159883657436 1204 46-47 -0.12841037462434457 1204 48-49 -0.10210296709521316 1204 50-51 -0.055214701441272496 1204 52-53 -0.1508614806291746 1204 54-55 -0.03970432890491793 1204 56-57 -0.061317293357909364 1204 58-59 -0.025059700400397844 1204 60-61 -0.26145793794627536 1204 62-63 7.677051255541301E-4 1204 64-65 -0.06837537952272044 1204 66-67 0.055052101351890315 1204 68-69 -0.04117069095796211 1204 70-71 -0.0357225695626866 1204 72-73 -0.03200477604227103 1204 74-75 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0.06138052354818058 2401 46-47 0.015960666728368267 2401 48-49 0.12536574113651255 2401 50-51 0.09691391803441718 2401 52-53 0.09866208330488746 2401 54-55 0.07280487577156691 2401 56-57 0.1371373527060058 2401 58-59 0.09590961304561318 2401 60-61 0.05583297234339568 2401 62-63 0.14369677809662562 2401 64-65 0.07883030407494118 2401 66-67 0.0040654615133419725 2401 68-69 0.015987122784913765 2401 70-71 0.055154361462165014 2401 72-73 0.15172406725260856 2401 74-75 0.0019673471858254743 2401 76-77 -0.05443813748242832 2401 78-79 -0.018429407960169897 2401 80-81 0.13239490361068107 2401 82-83 -0.051134918602258495 2401 84-85 -0.06725422388845459 2401 86-87 -0.2851443466941035 2401 88-89 -1.0150807216787214 2401 90-91 -0.13560589986930438 2402 1 0.3347439325081538 2402 2 0.162123507075556 2402 3 0.2841843317620416 2402 4 0.2946946148930252 2402 5 0.2038603171893527 2402 6 0.18043045129798685 2402 7 0.13634669899403207 2402 8 0.16655130006129326 2402 9 0.17106741900600042 2402 10-11 0.06892571211936627 2402 12-13 0.06062948435668858 2402 14-15 0.10482745289974815 2402 16-17 0.053376706405920515 2402 18-19 0.07637710172927825 2402 20-21 0.06786821761188833 2402 22-23 0.06432675584883185 2402 24-25 2.3117492185065203E-4 2402 26-27 -6.28334793638885E-4 2402 28-29 0.050346222253104145 2402 30-31 -0.04596172177810587 2402 32-33 0.00854463405401873 2402 34-35 0.03162565369681403 2402 36-37 0.07802565948435358 2402 38-39 0.01573366801198972 2402 40-41 0.04332276773197208 2402 42-43 0.049314600663848296 2402 44-45 0.09098590393061556 2402 46-47 0.04792711025106655 2402 48-49 0.02757810799480609 2402 50-51 0.12247199376753315 2402 52-53 0.06580060447756608 2402 54-55 0.03813382595970438 2402 56-57 0.030696534896577532 2402 58-59 0.10329013750083504 2402 60-61 0.04000142446409427 2402 62-63 0.07958872700116615 2402 64-65 0.2004887430806832 2402 66-67 0.10744764402830498 2402 68-69 0.09039486596072521 2402 70-71 0.1925138551485972 2402 72-73 0.2143743297549605 2402 74-75 0.13308354486147778 2402 76-77 0.0820036972414826 2402 78-79 0.24085241745802222 2402 80-81 0.2866598220135046 2402 82-83 0.16484991666536075 2402 84-85 -0.11103249286357197 2402 86-87 0.12104311212493002 2402 88-89 0.47026726366926397 2402 90-91 -0.362878627142031 2403 1 -0.264781262127876 2403 2 -0.2112922169825211 2403 3 -0.11558489916182424 2403 4 0.05044658772070676 2403 5 0.15442454222002766 2403 6 0.05346189525976541 2403 7 0.1333448248328608 2403 8 0.12790485048105182 2403 9 0.1729154477864725 2403 10-11 0.1709137166838275 2403 12-13 0.11525689347796231 2403 14-15 0.048971821604943955 2403 16-17 0.10693737810206727 2403 18-19 0.057615388221982755 2403 20-21 0.04975780358778081 2403 22-23 0.06503367041133856 2403 24-25 -0.002149552509983721 2403 26-27 0.09005238707668894 2403 28-29 0.11173387878777419 2403 30-31 0.0240348810116231 2403 32-33 0.022906314833988972 2403 34-35 0.05500550145048777 2403 36-37 0.0017654677004088626 2403 38-39 0.09866274625711924 2403 40-41 0.0067980427817815325 2403 42-43 0.0074259239456040405 2403 44-45 0.04425592194852612 2403 46-47 0.005872302565002485 2403 48-49 0.07161753362874634 2403 50-51 0.03627347446005302 2403 52-53 0.05611978249407912 2403 54-55 0.0724883344286269 2403 56-57 0.018436360589376477 2403 58-59 0.07884753154928603 2403 60-61 0.16358749907071513 2403 62-63 0.25841299751624547 2403 64-65 0.07312707785084172 2403 66-67 0.14355323428103617 2403 68-69 0.11023295868211846 2403 70-71 0.1990882211246614 2403 72-73 0.2316243336485968 2403 74-75 0.2265468681169267 2403 76-77 0.36017265433263645 2403 78-79 0.13893341255998592 2403 80-81 0.2688550722142793 2403 82-83 0.1691690443903866 2403 84-85 0.3442779565059979 2403 86-87 0.096153508436565 2403 88-89 0.6131244065264028 2403 90-91 -0.03560589986930296 2404 1 -0.5183940494324233 2404 2 -0.2045291152911517 2404 3 -0.45438085637497494 2404 4 -0.21899319746795243 2404 5 -0.20978814198953444 2404 6 -0.13397087426872645 2404 7 -0.12295927496695924 2404 8 -0.08038492973797418 2404 9 -0.10959082264988496 2404 10-11 0.006052754973765673 2404 12-13 0.09115056151077994 2404 14-15 0.07193106206066346 2404 16-17 0.04676366811721522 2404 18-19 -0.03924144880705427 2404 20-21 -0.003196612692505596 2404 22-23 -0.015843443581353256 2404 24-25 -0.0067421058254950594 2404 26-27 0.05314799588697383 2404 28-29 0.033657827832669795 2404 30-31 -0.005220249991516823 2404 32-33 0.10728207314947724 2404 34-35 0.07161505595396278 2404 36-37 0.10661131074112973 2404 38-39 0.07857351000745894 2404 40-41 0.07161431907081806 2404 42-43 0.05805156247070897 2404 44-45 0.0644805648803839 2404 46-47 0.1282564639930186 2404 48-49 0.17340538382030246 2404 50-51 0.1791849119859492 2404 52-53 0.14093975742505904 2404 54-55 0.055309373578197096 2404 56-57 0.0822283351140527 2404 58-59 0.12369488623396308 2404 60-61 0.02657003726075402 2404 62-63 0.13064972169044609 2404 64-65 0.20390040299337642 2404 66-67 0.23809769409513848 2404 68-69 0.13456460998528286 2404 70-71 0.06055762077908611 2404 72-73 0.1488226180055321 2404 74-75 0.10045262060548765 2404 76-77 0.11965989635029217 2404 78-79 -0.001851341616962543 2404 80-81 -0.011445730351546501 2404 82-83 -0.25055231839992587 2404 84-85 0.12650586217932158 2404 86-87 0.17285737972867565 2404 88-89 0.18079357945872943 2404 90-91 -0.025849802308329117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 26.0 31 470.0 32 3174.0 33 5733.0 34 7733.0 35 9701.0 36 13899.0 37 22108.0 38 60677.0 39 352298.0 40 319309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.1956685783278 11.809663950095583 32.56338665861757 29.43128081295905 2 27.075378199033057 17.308359871409813 29.410351610647428 26.205910318909698 3 26.673810269432046 19.377399804053994 22.46461841464841 31.48417151186555 4 26.251119316638327 20.457083639363724 21.066922558380536 32.22487448561741 5 26.741983680614943 20.893868660140257 20.633910515036572 31.73023714420823 6 27.513809097403186 23.318761256049342 23.388184040808525 25.77924560573895 7 25.82565322815949 22.91517843667938 23.296626455111628 27.962541880049503 8 24.92089323983057 24.360857622923604 23.864459558712557 26.853789578533267 9 24.846691350323468 24.516178527230835 24.290680242677908 26.346449879767786 10-11 24.646094716825466 23.35271805294242 24.19849382740892 27.80269340282319 12-13 24.98824088700184 23.14507852823696 24.067634393456146 27.79904619130505 14-15 25.404085883027637 22.73828867804932 23.95859534565504 27.899030093268003 16-17 25.318627944180054 22.50574750228894 23.827547262830638 28.348077290700363 18-19 25.374845307925263 22.728416053767443 23.846789447736715 28.049949190570572 20-21 25.45476954653842 22.775829803503335 23.751333118692838 28.018067531265405 22-23 25.163307039872823 22.36690193277057 23.96079624915737 28.50899477819923 24-25 25.21122385326639 22.59447535491141 24.136616997514864 28.05768379430733 26-27 25.491304544677085 22.685844291736675 24.015190007143502 27.807661156442737 28-29 25.34340382932056 22.38608123471944 24.09058667283758 28.179928263122417 30-31 25.536049139567083 22.615368942918266 24.08872589523885 27.759856022275798 32-33 25.63828020662961 22.67495202599989 24.041433635592508 27.645334131777993 34-35 25.50325372444092 22.36012288177584 24.195157400984378 27.941465992798864 36-37 25.601448278834727 22.63359872796674 24.25696002386431 27.50799296933423 38-39 25.75513592090632 22.59741128799172 24.145484418892387 27.501968372209575 40-41 25.618833356046917 22.374514916119864 24.288934613932437 27.717717113900786 42-43 25.645654355051107 22.63176459522167 24.33647394494262 27.386107104784603 44-45 25.91594447821245 22.67938481235815 24.152839981392123 27.251830728037273 46-47 25.72352170609195 22.35790060138472 24.355199571220275 27.56337812130305 48-49 25.86316436414093 22.657758204633204 24.21931557673745 27.259761854488417 50-51 26.1355250460211 22.55003527608606 24.080186192858825 27.23425348503402 52-53 25.888874889756835 22.427814692880347 24.253605841655208 27.429704575707607 54-55 25.985198944993183 22.76373681985699 24.152154369169033 27.09890986598079 56-57 26.154256218226262 22.634603632770343 24.063657994305107 27.147482154698288 58-59 26.165838980111257 22.335831857105948 24.11672592988051 27.38160323290229 60-61 26.217595485617984 22.67605900509383 23.98091865654213 27.12542685274606 62-63 26.550043781838344 22.620733464240153 23.780294136743226 27.048928617178277 64-65 26.33403518144607 22.50948673428502 23.84823582096588 27.308242263303033 66-67 26.51616092866312 22.670748929736153 23.577507942455167 27.235582199145565 68-69 26.5714653222125 22.649075771829516 23.52298779549702 27.256471110460968 70-71 26.530257210207225 22.42898522150348 23.595144001182973 27.44561356710632 72-73 26.600176617703564 22.615155416838004 23.555543571317823 27.22912439414061 74-75 26.80615369986648 22.516154848831818 23.486040071156612 27.19165138014509 76-77 26.853342270600955 22.429394799076693 23.1615269510543 27.555735979268057 78-79 26.968350661842166 22.322859890990703 23.122108734484495 27.586680712682636 80-81 27.253403476344506 22.462588419294065 22.716382895531936 27.567625208829487 82-83 27.691831119371706 21.87357641356439 22.40018950782631 28.034402959237596 84-85 28.070906683665964 21.737851223459028 22.10317265728436 28.088069435590647 86-87 28.207222241948653 21.064872350246777 21.812306927529026 28.91559848027554 88-89 27.862223665181073 20.539535432838463 21.678817036096927 29.91942386588353 90-91 25.97724620770128 21.317094515752625 24.102975495915985 28.602683780630105 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 3.0 11 6.0 12 8.5 13 11.0 14 18.0 15 24.0 16 36.5 17 63.5 18 97.0 19 147.5 20 234.0 21 347.0 22 467.5 23 606.0 24 807.0 25 1042.5 26 1356.0 27 1766.0 28 2280.5 29 2916.0 30 3622.0 31 4328.0 32 5208.5 33 6277.5 34 7600.0 35 9268.5 36 11121.0 37 12988.0 38 15067.0 39 17639.5 40 20565.0 41 23910.5 42 27203.5 43 30609.5 44 34355.5 45 38324.0 46 42643.0 47 46577.0 48 50190.5 49 53744.5 50 56887.5 51 59722.0 52 61623.0 53 62481.5 54 62774.5 55 62338.5 56 61414.5 57 59914.5 58 57557.0 59 54674.0 60 51678.5 61 48321.0 62 44911.0 63 41383.5 64 37571.0 65 34079.5 66 30308.5 67 26654.5 68 23382.0 69 20055.5 70 16896.0 71 13993.5 72 11421.0 73 9092.5 74 7053.5 75 5507.0 76 4247.0 77 3080.5 78 2229.0 79 1630.0 80 1144.0 81 765.5 82 513.0 83 326.5 84 209.0 85 148.5 86 105.5 87 73.5 88 50.0 89 29.5 90 18.5 91 17.5 92 13.0 93 6.5 94 3.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010061273153504843 2 0.005533700234427665 3 0.0013834250586069162 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0017753475242778772 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9872.0 32-33 22353.0 34-35 25322.0 36-37 27765.0 38-39 30305.0 40-41 33071.0 42-43 34593.0 44-45 35583.0 46-47 36718.0 48-49 36897.0 50-51 37234.0 52-53 36606.0 54-55 35418.0 56-57 34656.0 58-59 33718.0 60-61 32220.0 62-63 30849.0 64-65 29466.0 66-67 27469.0 68-69 26300.0 70-71 24687.0 72-73 22496.0 74-75 20802.0 76-77 18956.0 78-79 17358.0 80-81 15841.0 82-83 14428.0 84-85 12997.0 86-87 11557.0 88-89 10371.0 90-91 9220.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.84280480832757 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8512061151058 99.69424482026592 2 0.14105364678797871 0.2816638344750988 3 0.006831677105338045 0.020462814112253598 4 9.085610008895734E-4 0.0036285311467310556 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0010061273153504845 0.0 0.0 0.0 0.0 2 0.0010061273153504845 0.0 0.0 0.0 0.0 3 0.0010061273153504845 0.0 0.0 0.0 1.2576591441881057E-4 4 0.0010061273153504845 0.0 0.0 0.0 1.2576591441881057E-4 5 0.001131893229769295 0.0 0.0 0.0 1.2576591441881057E-4 6 0.001131893229769295 0.0 0.0 0.0 1.2576591441881057E-4 7 0.0013834250586069162 0.0 0.0 0.0 1.2576591441881057E-4 8 0.004024509261401938 0.0 0.0 0.0 1.2576591441881057E-4 9 0.004024509261401938 0.0 0.0 0.0 1.2576591441881057E-4 10-11 0.004024509261401938 0.0 0.0 0.0 1.2576591441881057E-4 12-13 0.004024509261401938 0.0 0.0 0.0 1.2576591441881057E-4 14-15 0.004024509261401938 0.0 0.0 0.0 2.5153182883762114E-4 16-17 0.004087392218611343 0.0 0.0 0.0 2.5153182883762114E-4 18-19 0.004150275175820749 6.288295720940528E-5 0.0 0.0 2.5153182883762114E-4 20-21 0.004150275175820749 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 22-23 0.0042131581330301544 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 24-25 0.00452757291907718 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 26-27 0.0047791047479148014 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 28-29 0.004967753619543018 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 30-31 0.0055965831916370695 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 32-33 0.005973880934893502 1.2576591441881057E-4 0.0 0.0 2.5153182883762114E-4 34-35 0.006288295720940528 1.2576591441881057E-4 0.0 1.2576591441881057E-4 2.5153182883762114E-4 36-37 0.006602710506987554 1.2576591441881057E-4 0.0 1.2576591441881057E-4 2.5153182883762114E-4 38-39 0.00679135937861577 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 40-41 0.007294423036291012 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 42-43 0.007545954865128633 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 44-45 0.007797486693966254 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 46-47 0.00798613556559447 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 48-49 0.008237667394432092 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 50-51 0.008489199223269713 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 52-53 0.008740731052107334 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 54-55 0.008992262880944955 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 56-57 0.009369560624201387 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 58-59 0.009558209495829601 1.2576591441881057E-4 0.0 1.2576591441881057E-4 5.030636576752423E-4 60-61 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1.2576591441881057E-4 5.030636576752423E-4 >>END_MODULE