##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-18A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 674322 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.29502225939536 40.0 40.0 40.0 40.0 40.0 2 39.154415842876254 40.0 40.0 40.0 40.0 40.0 3 39.1412025708786 40.0 40.0 40.0 40.0 40.0 4 39.04489546537114 40.0 40.0 40.0 40.0 40.0 5 39.1839151028737 40.0 40.0 40.0 40.0 40.0 6 39.26038895364529 40.0 40.0 40.0 40.0 40.0 7 39.27860576994374 40.0 40.0 40.0 40.0 40.0 8 39.30964731982643 40.0 40.0 40.0 40.0 40.0 9 39.32292287660791 40.0 40.0 40.0 40.0 40.0 10-11 39.31585800255664 40.0 40.0 40.0 40.0 40.0 12-13 39.31261029597136 40.0 40.0 40.0 40.0 40.0 14-15 39.323874944017845 40.0 40.0 40.0 40.0 40.0 16-17 39.46343438298024 40.0 40.0 40.0 40.0 40.0 18-19 39.47497486364081 40.0 40.0 40.0 40.0 40.0 20-21 39.48328246742654 40.0 40.0 40.0 40.0 40.0 22-23 39.487129294313405 40.0 40.0 40.0 40.0 40.0 24-25 39.49205572411993 40.0 40.0 40.0 40.0 40.0 26-27 39.48142282173798 40.0 40.0 40.0 40.0 40.0 28-29 39.47757896079321 40.0 40.0 40.0 40.0 40.0 30-31 39.4587253954433 40.0 40.0 40.0 40.0 40.0 32-33 39.4647651066914 40.0 40.0 40.0 40.0 40.0 34-35 39.45328898301098 40.0 40.0 40.0 40.0 40.0 36-37 39.45531134608285 40.0 40.0 40.0 40.0 40.0 38-39 39.4435353987822 40.0 40.0 40.0 40.0 40.0 40-41 39.426691047702136 40.0 40.0 40.0 40.0 40.0 42-43 39.42821317415974 40.0 40.0 40.0 40.0 40.0 44-45 39.41102522816409 40.0 40.0 40.0 40.0 40.0 46-47 39.40880065700022 40.0 40.0 40.0 40.0 40.0 48-49 39.39823990205137 40.0 40.0 40.0 40.0 40.0 50-51 39.393703267767506 40.0 40.0 40.0 40.0 40.0 52-53 39.38001068504745 40.0 40.0 40.0 40.0 40.0 54-55 39.35851512094759 40.0 40.0 40.0 40.0 40.0 56-57 39.36384702982437 40.0 40.0 40.0 40.0 40.0 58-59 39.34293545104427 40.0 40.0 40.0 40.0 40.0 60-61 39.31313356933397 40.0 40.0 40.0 40.0 40.0 62-63 39.38674681157407 40.0 40.0 40.0 40.0 40.0 64-65 39.380563275971475 40.0 40.0 40.0 40.0 40.0 66-67 39.38839250609046 40.0 40.0 40.0 40.0 40.0 68-69 39.37776126456897 40.0 40.0 40.0 40.0 40.0 70-71 39.36036933026149 40.0 40.0 40.0 40.0 40.0 72-73 39.36997892494584 40.0 40.0 40.0 40.0 40.0 74-75 39.35418273002739 40.0 40.0 40.0 40.0 40.0 76-77 39.37492179850536 40.0 40.0 40.0 40.0 40.0 78-79 39.3500227191015 40.0 40.0 40.0 40.0 40.0 80-81 39.340443867314804 40.0 40.0 40.0 40.0 40.0 82-83 39.32298552272627 40.0 40.0 40.0 40.0 40.0 84-85 39.33474625195597 40.0 40.0 40.0 40.0 40.0 86-87 39.35166539605339 40.0 40.0 40.0 40.0 40.0 88-89 39.357687575586866 40.0 40.0 40.0 40.0 40.0 90-91 39.41994143399484 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2023709237522482 1101 2 0.07658555517685528 1101 3 -0.0622169441594167 1101 4 0.06003617008087048 1101 5 0.023862920401832355 1101 6 -9.710671253415626E-4 1101 7 0.1583148764935629 1101 8 0.04791560657552196 1101 9 0.05634402136522709 1101 10-11 0.010361107923422708 1101 12-13 -0.03848940900189035 1101 14-15 0.04047959908892551 1101 16-17 -0.043533679228850986 1101 18-19 -0.02596374429722914 1101 20-21 0.0046575229247523 1101 22-23 0.024904622564910994 1101 24-25 0.04944827042818645 1101 26-27 -0.04946596295557981 1101 28-29 0.0025064149356381904 1101 30-31 -0.08304408990000667 1101 32-33 -0.03295359221863947 1101 34-35 -0.010413440068894886 1101 36-37 0.05681065527067375 1101 38-39 -0.021837669879843702 1101 40-41 -0.07213751372810862 1101 42-43 0.11939860660331902 1101 44-45 0.012742261151643675 1101 46-47 0.09053549396435301 1101 48-49 0.0364124354219868 1101 50-51 0.047502666694228424 1101 52-53 0.01113863388522418 1101 54-55 0.07599611498911685 1101 56-57 -0.03860843419135307 1101 58-59 0.09380407657658907 1101 60-61 0.13090855880739127 1101 62-63 -0.10665764084048845 1101 64-65 0.14871187357041293 1101 66-67 -0.10667853463345267 1101 68-69 -0.08008517403710869 1101 70-71 -0.14420001347563982 1101 72-73 -0.11470484373973022 1101 74-75 -0.22105904419723998 1101 76-77 -0.4082749655361937 1101 78-79 -0.32384214814941714 1101 80-81 0.030228612467624316 1101 82-83 0.35872177636152003 1101 84-85 -0.22118318090764433 1101 86-87 -0.15294604900078212 1101 88-89 -0.07646432200184705 1101 90-91 -1.4150562501660744 1102 1 0.28781409947007575 1102 2 0.12774145642633528 1102 3 0.1693313053363994 1102 4 0.015231229653281275 1102 5 -0.04714649326533049 1102 6 -0.01390249389540088 1102 7 -0.06496591247647387 1102 8 0.0175088153341747 1102 9 -0.08374643531040249 1102 10-11 -0.10377350606610491 1102 12-13 -0.12794101045964368 1102 14-15 -0.1277212785703341 1102 16-17 0.05473342516828694 1102 18-19 0.027367839169428976 1102 20-21 -0.03825663820104097 1102 22-23 -0.15276927703055065 1102 24-25 0.011559480170809877 1102 26-27 -0.054958258046411856 1102 28-29 0.013295134362977024 1102 30-31 -0.02503337174681519 1102 32-33 -0.013630107148458137 1102 34-35 0.040330375530921 1102 36-37 -0.03803057873656002 1102 38-39 0.05286522695285356 1102 40-41 0.043017731388808045 1102 42-43 -0.09973053564426237 1102 44-45 -0.05344661250890681 1102 46-47 -0.01528688980596371 1102 48-49 -0.08122752432707614 1102 50-51 -0.026697341102014605 1102 52-53 -0.09550380544824577 1102 54-55 0.04839387214796176 1102 56-57 9.925758375999294E-4 1102 58-59 0.08459765315359391 1102 60-61 -0.06396197885992905 1102 62-63 -0.3433883715005379 1102 64-65 -0.2267676081164538 1102 66-67 -0.23325373147201844 1102 68-69 -0.16296394029126304 1102 70-71 -0.15022916985305557 1102 72-73 -0.06629871809462173 1102 74-75 -0.12543295820427858 1102 76-77 0.05192756664516196 1102 78-79 0.11155906729837994 1102 80-81 -0.03333850214156797 1102 82-83 -0.1655443731531534 1102 84-85 -0.43305290247766237 1102 86-87 -0.3252346020608101 1102 88-89 0.3459155046333038 1102 90-91 0.09630738619756585 1103 1 0.38089180131770917 1103 2 0.2691508042707653 1103 3 0.10729532288470267 1103 4 0.018874821992767465 1103 5 -0.14233863871373842 1103 6 -0.028323948379501473 1103 7 0.12622513620318188 1103 8 0.015879480988438388 1103 9 0.0034624991365674873 1103 10-11 0.025798121369987825 1103 12-13 0.07998367105558657 1103 14-15 0.0929348844263842 1103 16-17 -3.7920454570894435E-4 1103 18-19 -0.009447734946832043 1103 20-21 -0.006624351016355945 1103 22-23 -0.0037510959793465304 1103 24-25 -0.006995948834500609 1103 26-27 -0.006315509375184547 1103 28-29 0.05604538746345611 1103 30-31 0.10134022565468825 1103 32-33 0.08553196984085787 1103 34-35 0.06024400041469136 1103 36-37 0.015438688362074515 1103 38-39 -0.10881519751890067 1103 40-41 -0.017556838110579065 1103 42-43 -0.06258387542288091 1103 44-45 0.1027117510280533 1103 46-47 0.05667855016776002 1103 48-49 0.12670602420205768 1103 50-51 -0.06014495660159014 1103 52-53 0.035918632636011694 1103 54-55 0.05065670132686506 1103 56-57 0.10787229284259325 1103 58-59 -0.018029544993801494 1103 60-61 -0.046310122877102344 1103 62-63 -0.031248519425659538 1103 64-65 0.018021409266246735 1103 66-67 -0.0107831320166909 1103 68-69 0.20480796287210978 1103 70-71 0.05186861471008086 1103 72-73 -0.10101092118408417 1103 74-75 0.12713293658223535 1103 76-77 -0.377436739934339 1103 78-79 0.3472728109918819 1103 80-81 0.12537068581735866 1103 82-83 -0.03954990484369603 1103 84-85 0.1879254544859279 1103 86-87 0.4059275946186247 1103 88-89 0.3210063193712358 1103 90-91 -0.6600562501660789 1104 1 0.2806502554239927 1104 2 0.21395137407133547 1104 3 0.15040971654257618 1104 4 0.1840627942459605 1104 5 0.11630901395565019 1104 6 0.10894689269569113 1104 7 0.0807107673192462 1104 8 0.07410597671493946 1104 9 0.06837795807376779 1104 10-11 0.06567641940046087 1104 12-13 -0.05613750446776322 1104 14-15 -0.045884327762401256 1104 16-17 0.11825699017744284 1104 18-19 -0.044828014892324575 1104 20-21 -0.07793458265268072 1104 22-23 0.029562108529511022 1104 24-25 -0.06252827882335055 1104 26-27 -0.009450960880201364 1104 28-29 0.04962351826985412 1104 30-31 0.002395440568598417 1104 32-33 -0.12451935232542155 1104 34-35 0.07425556203767059 1104 36-37 0.01125161655950535 1104 38-39 0.019766941568633456 1104 40-41 0.01456446866686889 1104 42-43 0.019094740594667314 1104 44-45 0.036915543489826064 1104 46-47 -0.1808516923091048 1104 48-49 0.035907478525125214 1104 50-51 0.06918396068803645 1104 52-53 -0.06975597484824902 1104 54-55 -0.02478416404925099 1104 56-57 -0.15429637900215454 1104 58-59 0.029352527572228837 1104 60-61 -0.0883692993339551 1104 62-63 -0.02637984741370758 1104 64-65 -0.09411831404728588 1104 66-67 -0.043504682088467916 1104 68-69 0.16888681571884945 1104 70-71 0.010670048270512211 1104 72-73 0.1218850287342832 1104 74-75 0.1676620940661877 1104 76-77 0.43102689868566557 1104 78-79 -0.06437309854860729 1104 80-81 0.14964714170260152 1104 82-83 0.03205092227496209 1104 84-85 -0.1695348292592911 1104 86-87 0.32872363009072103 1104 88-89 0.08835049281297103 1104 90-91 0.13994374983392532 1201 1 0.21611768654359764 1201 2 0.18977554989550782 1201 3 0.13190358499296195 1201 4 0.1354244760424237 1201 5 0.12427206539696556 1201 6 0.22995342239786964 1201 7 0.1072436547216995 1201 8 0.041138943747903056 1201 9 0.0023483355224058755 1201 10-11 0.08552034359220784 1201 12-13 -0.0917323444104241 1201 14-15 0.030465409456908787 1201 16-17 -0.020953075119578557 1201 18-19 0.01040370990462236 1201 20-21 -0.026684383576387916 1201 22-23 0.004526274798024588 1201 24-25 0.008342879004317183 1201 26-27 -0.06020745076713041 1201 28-29 0.07389489906296376 1201 30-31 -0.04936439379992663 1201 32-33 -0.029593612302683425 1201 34-35 0.030940846111001008 1201 36-37 0.001662688891329367 1201 38-39 0.030711694831140335 1201 40-41 0.05257232743687723 1201 42-43 0.047688758309405443 1201 44-45 -0.06157620266628783 1201 46-47 -0.1276755461479695 1201 48-49 -0.012859097649467799 1201 50-51 0.0986966259360571 1201 52-53 0.06139171953023492 1201 54-55 0.06009199485333028 1201 56-57 0.12315952765358418 1201 58-59 -0.04983821380523068 1201 60-61 0.08320327921926918 1201 62-63 -0.11055889234388872 1201 64-65 -0.1275654282790839 1201 66-67 -0.025044613454006992 1201 68-69 -0.02724137064719656 1201 70-71 -0.03910844887017362 1201 72-73 -0.22633074597369784 1201 74-75 -0.07906103034400758 1201 76-77 -0.1782221855634205 1201 78-79 -0.6169058695879315 1201 80-81 -0.007671819680744818 1201 82-83 0.14399745527412477 1201 84-85 -0.4904904869926625 1201 86-87 0.015810436644329684 1201 88-89 0.031279785742263755 1201 90-91 0.4599437498339256 1202 1 0.19448059787049488 1202 2 0.11133044928974556 1202 3 0.2499943584853952 1202 4 0.029071549190881285 1202 5 -0.047777491694148466 1202 6 0.05734984868626469 1202 7 0.13993063533111894 1202 8 0.13325790511894553 1202 9 -0.00547486620661175 1202 10-11 0.009620066583899245 1202 12-13 0.08243891369435374 1202 14-15 6.641857498621562E-4 1202 16-17 0.015487215891347716 1202 18-19 0.10483614727334611 1202 20-21 0.055285723878476745 1202 22-23 -0.0317892102709223 1202 24-25 0.05732768920796616 1202 26-27 0.12116602340412896 1202 28-29 0.013695818365278 1202 30-31 0.05105872878385043 1202 32-33 0.052619331767729705 1202 34-35 -0.05039763420548837 1202 36-37 0.02331602077710926 1202 38-39 0.07204773521372232 1202 40-41 0.11664784330515943 1202 42-43 0.13192226060206735 1202 44-45 0.039085416058213696 1202 46-47 -0.024691064071205915 1202 48-49 0.01617033185961958 1202 50-51 -0.09514675262165895 1202 52-53 0.0717532297601906 1202 54-55 0.07368705521441399 1202 56-57 -0.014060514336911467 1202 58-59 0.0899554527064339 1202 60-61 0.016968406255671198 1202 62-63 0.014355921155299711 1202 64-65 0.01667001400789303 1202 66-67 0.034752686661775556 1202 68-69 0.1141124418466859 1202 70-71 0.027235067244710365 1202 72-73 0.048152286033911196 1202 74-75 -0.09095806067853118 1202 76-77 -0.2011430080650456 1202 78-79 -0.1083662543714965 1202 80-81 -0.3481643283877105 1202 82-83 -0.6578601298651989 1202 84-85 -0.05286079448181624 1202 86-87 -0.5183399565145947 1202 88-89 0.5161282705907482 1202 90-91 0.4599437498339256 1203 1 0.2781048760829279 1203 2 0.060949600871971654 1203 3 0.13489269248261593 1203 4 0.14288638844396218 1203 5 0.030642314248005675 1203 6 0.1558626394157585 1203 7 0.022282074041129363 1203 8 0.04843680505235426 1203 9 0.011870536702126344 1203 10-11 0.057638894635019255 1203 12-13 0.12316856148937916 1203 14-15 0.042158875156957265 1203 16-17 -0.02583962654838956 1203 18-19 0.05925474909594186 1203 20-21 0.044156740173626474 1203 22-23 0.04898653462601743 1203 24-25 0.08573639657346632 1203 26-27 0.018359755901059316 1203 28-29 0.045842161460285524 1203 30-31 0.044578984446410175 1203 32-33 0.06903895814797067 1203 34-35 0.05204655265621483 1203 36-37 0.09019617782532663 1203 38-39 0.15183934243191288 1203 40-41 -0.028740737384808313 1203 42-43 0.027006598966963224 1203 44-45 -0.05183665566479334 1203 46-47 -0.022901627059930263 1203 48-49 0.043212703287380805 1203 50-51 -0.06755355306772515 1203 52-53 -0.13106344278565984 1203 54-55 0.018987541401159547 1203 56-57 -0.056773129266993294 1203 58-59 0.06734335588366491 1203 60-61 -0.03260688112808907 1203 62-63 0.10077676214292097 1203 64-65 0.035344063240479784 1203 66-67 -0.08734524285723921 1203 68-69 -0.13426612008139216 1203 70-71 -0.007278891536650178 1203 72-73 0.11257167890158826 1203 74-75 -0.18004077976125643 1203 76-77 -0.3964039892813389 1203 78-79 -0.5366948265697786 1203 80-81 -0.18538130728757096 1203 82-83 -0.2324310101895648 1203 84-85 -0.26580063242165153 1203 86-87 0.19342000506185997 1203 88-89 -0.055300300837821226 1203 90-91 0.4599437498339256 1204 1 0.15311402353993486 1204 2 0.11308254592977818 1204 3 -0.09267789413397054 1204 4 -0.03206258235372417 1204 5 -0.1509436280005474 1204 6 -0.12625400653683272 1204 7 -0.13022191001659422 1204 8 -0.11271326464975573 1204 9 -0.20667294622614918 1204 10-11 -0.09905265916261641 1204 12-13 -0.11480321293583984 1204 14-15 -0.03193885294736276 1204 16-17 -0.20097608241531617 1204 18-19 -0.0031039945121804635 1204 20-21 -0.05901568863496465 1204 22-23 0.01195520949968909 1204 24-25 -0.12417914442679034 1204 26-27 -0.12790641846609674 1204 28-29 -0.18187357488731948 1204 30-31 -0.07741513094158137 1204 32-33 -0.20342850521835487 1204 34-35 -0.17013092734867286 1204 36-37 -0.16684036686088177 1204 38-39 -0.08689686635493388 1204 40-41 -0.1763058228556531 1204 42-43 -0.1086372673503675 1204 44-45 -0.1916337193822244 1204 46-47 -0.0933308760495919 1204 48-49 -0.1386104023836694 1204 50-51 -0.11093144513493769 1204 52-53 -0.17174969672873885 1204 54-55 -0.2197236484943872 1204 56-57 -0.18115831302965546 1204 58-59 -0.16228417520902383 1204 60-61 -0.25633434147299994 1204 62-63 -0.11576145845717889 1204 64-65 0.06300553910116236 1204 66-67 -0.2479553442826088 1204 68-69 -0.24041231449348288 1204 70-71 -0.13419612445067486 1204 72-73 -0.1927661698324954 1204 74-75 -0.07635564948037654 1204 76-77 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54-55 0.06737470482105579 2401 56-57 0.16615404625783725 2401 58-59 0.09144624238957277 2401 60-61 0.1674597914138829 2401 62-63 0.1208149480648757 2401 64-65 -0.0361990608089755 2401 66-67 0.1552754156916265 2401 68-69 0.0073579436193966785 2401 70-71 0.13382287615963406 2401 72-73 0.17757714127546365 2401 74-75 0.06247485275437725 2401 76-77 0.2807103337035102 2401 78-79 0.14295970439414418 2401 80-81 0.14347547431580665 2401 82-83 0.23284383156989463 2401 84-85 0.3515153558620767 2401 86-87 0.2956599011826171 2401 88-89 0.3827949372574153 2401 90-91 0.4599437498339256 2402 1 0.39078838151151274 2402 2 0.29433403403944425 2402 3 0.252712224925574 2402 4 0.28246406140817015 2402 5 0.21435015312854944 2402 6 0.21545583551076675 2402 7 0.1816276985230303 2402 8 0.20298296597518828 2402 9 0.18743731771737515 2402 10-11 0.15051838817570484 2402 12-13 0.15268397450726212 2402 14-15 0.14117120737427058 2402 16-17 0.11910760725631775 2402 18-19 0.10384511402259733 2402 20-21 0.04106155839716763 2402 22-23 0.06980968126250531 2402 24-25 0.07425975446363253 2402 26-27 0.034267530645664124 2402 28-29 0.0900058120061118 2402 30-31 0.07783222655272937 2402 32-33 0.017128168729634297 2402 34-35 0.08079069991844534 2402 36-37 -0.0156639294159433 2402 38-39 1.0977843903958728E-4 2402 40-41 0.049463219941202397 2402 42-43 0.08766205807401661 2402 44-45 0.07834669303440478 2402 46-47 0.11245568273615447 2402 48-49 0.12059678878495816 2402 50-51 0.15378713985013093 2402 52-53 0.2349902127093202 2402 54-55 0.1917750763316306 2402 56-57 0.2771428657452617 2402 58-59 0.13963642515214048 2402 60-61 0.21444232127937113 2402 62-63 0.2548588802656724 2402 64-65 0.30379355381197826 2402 66-67 0.31204286559993477 2402 68-69 0.2087404401942976 2402 70-71 0.24857703683326804 2402 72-73 0.3614629120404089 2402 74-75 0.2989480030949352 2402 76-77 0.11303103824268135 2402 78-79 0.14364417973878574 2402 80-81 0.19384119252315912 2402 82-83 0.13125311459455702 2402 84-85 -0.03144073002896164 2402 86-87 -0.279141040450007 2402 88-89 0.3256520801145584 2402 90-91 -0.5400562501660744 2403 1 -0.1594694381960693 2403 2 -0.132143209311387 2403 3 0.09226864366310394 2403 4 0.04416658139469831 2403 5 0.007846118462538243 2403 6 -0.14502414241020034 2403 7 0.05855336196361094 2403 8 0.03155530704562182 2403 9 0.02684248544028378 2403 10-11 0.03760046685876972 2403 12-13 0.13907921690960734 2403 14-15 0.17936058208088923 2403 16-17 0.09134310327829809 2403 18-19 0.0630070387791335 2403 20-21 0.04270724174744345 2403 22-23 0.07793083633818298 2403 24-25 0.07392983442179002 2403 26-27 0.036089472648441756 2403 28-29 0.09643337753364989 2403 30-31 0.0469206770614079 2403 32-33 0.18699697760694534 2403 34-35 0.06851418599411829 2403 36-37 0.029006843794377346 2403 38-39 0.12298351956567899 2403 40-41 0.1401110938192005 2403 42-43 0.09728702066958306 2403 44-45 0.15025446164429468 2403 46-47 0.1565205734070929 2403 48-49 0.12281862157392709 2403 50-51 0.18694988687400382 2403 52-53 0.07084389373022759 2403 54-55 0.030389525018634345 2403 56-57 -0.008495513209240357 2403 58-59 0.0722613007769155 2403 60-61 0.09251321556211423 2403 62-63 0.04269931618473066 2403 64-65 -0.018854863007341294 2403 66-67 -0.11999152451760153 2403 68-69 0.13607945987563141 2403 70-71 -0.09158957267940337 2403 72-73 -0.017723557296989156 2403 74-75 -0.054259153979629104 2403 76-77 0.31492351638021177 2403 78-79 0.472694287152315 2403 80-81 0.1999915969018673 2403 82-83 0.41256672791810445 2403 84-85 0.4210133288109148 2403 86-87 0.4397900284810632 2403 88-89 0.12588436815172344 2403 90-91 -0.5349628715582639 2404 1 -0.4367662391097369 2404 2 -0.13860530061744214 2404 3 -0.33621300293822287 2404 4 -0.318221761844832 2404 5 -0.0947950783515239 2404 6 -0.16439375225057518 2404 7 -0.0745253680670217 2404 8 -0.1355383766142353 2404 9 0.0559843866092109 2404 10-11 0.009583688513025379 2404 12-13 0.07040119605451167 2404 14-15 0.004958510021843665 2404 16-17 -0.013350750831271796 2404 18-19 -0.033228903137036525 2404 20-21 -0.011857495516593985 2404 22-23 -0.032153846934242836 2404 24-25 0.0032155227761592187 2404 26-27 -0.05326896630836586 2404 28-29 0.024224060052617347 2404 30-31 0.03710880854812615 2404 32-33 0.0344865623006001 2404 34-35 -0.016839497734189024 2404 36-37 -0.01495559233129029 2404 38-39 0.03670133549648824 2404 40-41 0.13622542146408279 2404 42-43 0.02788938272467334 2404 44-45 0.04424517035023001 2404 46-47 -0.02364124863974837 2404 48-49 0.0102260124374709 2404 50-51 0.05514647202589629 2404 52-53 0.03336878200603621 2404 54-55 0.06857709743554352 2404 56-57 0.0684928574961603 2404 58-59 0.04379543667958785 2404 60-61 0.022740476116986486 2404 62-63 0.03223152432692444 2404 64-65 0.0845441974741945 2404 66-67 -0.047575040010130465 2404 68-69 0.010820173707259073 2404 70-71 0.10149260368728363 2404 72-73 0.3733577090611675 2404 74-75 0.20381110810133407 2404 76-77 -0.06662098399682037 2404 78-79 0.0013159343203241747 2404 80-81 0.19372382313667913 2404 82-83 0.11150731645929568 2404 84-85 0.34320235499860274 2404 86-87 0.11888041600519017 2404 88-89 0.10330775777023149 2404 90-91 0.4599437498339256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 16.0 31 345.0 32 2653.0 33 4848.0 34 6496.0 35 8030.0 36 11803.0 37 18400.0 38 49354.0 39 293569.0 40 278808.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.063267355071012 12.210056754059316 32.741026793195445 28.985649097674226 2 27.120509230696214 17.370734659010015 29.663844857861516 25.84491125243225 3 26.672321771511026 19.59493828570242 22.511074168380745 31.221665774405803 4 26.29218681876018 20.709690622580904 21.046621643665787 31.951500914993137 5 26.736040052081943 21.264173495748324 20.778648776103996 31.221137676065737 6 27.57080445247226 23.5236281776362 23.394461399746707 25.511105970144826 7 25.727916336705608 23.342409116119597 23.393571617120603 27.53610293005419 8 24.826121645148756 24.793644579295947 23.87939293097363 26.50084084458167 9 24.689836606250427 24.89700766102841 24.318352359851822 26.09480337286934 10-11 24.420825658958183 23.730502638205486 24.289656870159952 27.559014832676375 12-13 24.803580485287444 23.487888575487677 24.062391557742444 27.64613938148244 14-15 25.15689833640308 23.14287536221568 23.893926047200004 27.806300254181238 16-17 25.14577605357678 22.851916443479524 23.79212008506322 28.210187417880476 18-19 25.30096897327983 23.06286907441845 23.730576786757663 27.905585165544057 20-21 25.32402917300637 23.1729055258467 23.75526825463206 27.747797046514872 22-23 25.076743751501507 22.7117015313159 24.01968199168943 28.191872725493162 24-25 25.073258769549266 22.989981047630064 24.169684512740204 27.767075670080466 26-27 25.26648989651828 23.098089636701754 24.046004727711686 27.58941573906828 28-29 25.179291199160048 22.75315057198193 24.16123157779221 27.90632665106581 30-31 25.396937208548763 22.962186322655658 24.227387759066733 27.413488709728846 32-33 25.413904088275313 23.02462868447891 24.16827367526842 27.393193551977358 34-35 25.281421160244104 22.650132334601295 24.381702249101807 27.68674425605279 36-37 25.419430386736742 22.973903552936743 24.349131742556633 27.25753431776988 38-39 25.46014167068386 23.093033126811445 24.306663402078886 27.140161800425812 40-41 25.357820122208718 22.748816008577545 24.41951747737592 27.473846391837814 42-43 25.48155960754581 23.02205865793879 24.421790666076134 27.07459106843927 44-45 25.645818189060275 22.99752048833435 24.294932435796582 27.0617288868088 46-47 25.537070420679935 22.709025375070244 24.421659370462002 27.332244833787815 48-49 25.59450588663147 22.946747275573244 24.434622308207082 27.024124529588207 50-51 25.692156380624535 23.030735185640523 24.268379641013034 27.008728792721907 52-53 25.632209804734522 22.824343726961622 24.345726676327512 27.197719791976343 54-55 25.84691995412744 23.112444458010472 24.277281890949475 26.763353696912613 56-57 25.895106131793654 22.96455263413647 24.17284434481561 26.967496889254267 58-59 25.785785083372076 22.88333172524494 24.15465717017376 27.176226021209228 60-61 25.989244114291488 22.96169746301814 24.234172774431546 26.814885648258823 62-63 26.144665735295707 22.9983646858789 24.063376544613753 26.79359303421164 64-65 26.008735238614033 22.78311705997938 23.98688741464174 27.22126028676485 66-67 26.034093998808057 22.996558741702394 24.003669210762652 26.9656780487269 68-69 26.237883809642927 23.01419548496029 23.891876680632855 26.856044024763932 70-71 26.054103773754388 22.84696946287866 23.84151884362076 27.25740791974619 72-73 26.198221825030586 22.99475066712882 23.826208244417625 26.980819263422966 74-75 26.31666617674437 23.027327865800483 23.691662502327134 26.964343455128017 76-77 26.409732757614655 22.771618625277164 23.339362819465514 27.479285797642667 78-79 26.614826188168202 22.658157092216143 23.53906786196942 27.187948857646234 80-81 27.071179551864894 22.856992694591895 22.92531665527934 27.146511098263872 82-83 26.954141613569053 22.449071165754287 22.70708067845284 27.88970654222382 84-85 27.70735077653279 21.962209738952808 22.211541349995496 28.11889813451891 86-87 28.00513456475861 21.436807867197526 21.780562626460416 28.77749494158345 88-89 27.609525540810765 20.796218869296492 21.98509361934194 29.609161970550808 90-91 23.690707106330454 21.944983248104393 25.5863163463234 28.777993299241757 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 1.0 9 2.5 10 4.0 11 6.0 12 8.0 13 13.0 14 22.0 15 34.0 16 52.5 17 73.5 18 108.5 19 153.5 20 205.0 21 313.0 22 444.0 23 592.5 24 826.0 25 1086.5 26 1399.0 27 1834.5 28 2315.5 29 2853.5 30 3443.0 31 4118.0 32 4988.5 33 5939.0 34 7122.5 35 8511.0 36 10104.5 37 11888.0 38 13823.0 39 16169.0 40 18708.5 41 21525.5 42 24609.5 43 27796.0 44 31070.0 45 34315.5 46 37693.5 47 41189.5 48 44382.5 49 47315.5 50 50016.0 51 52131.5 52 53632.5 53 54427.0 54 54299.5 55 53551.0 56 52184.0 57 50223.0 58 47823.5 59 45172.5 60 42764.5 61 40074.5 62 37227.5 63 34033.5 64 30482.5 65 27439.5 66 24348.5 67 21416.0 68 18685.5 69 15906.0 70 13373.0 71 11131.0 72 9059.0 73 7082.5 74 5491.5 75 4307.5 76 3309.5 77 2419.0 78 1778.5 79 1276.0 80 861.0 81 595.5 82 417.5 83 280.0 84 193.5 85 134.0 86 91.0 87 61.0 88 36.5 89 22.0 90 12.5 91 13.0 92 10.5 93 6.0 94 4.0 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001334673939156664 2 0.007414855217537023 3 7.414855217537023E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.1659742768894875E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8980.0 32-33 20303.0 34-35 23175.0 36-37 24991.0 38-39 27258.0 40-41 29244.0 42-43 30411.0 44-45 31304.0 46-47 32173.0 48-49 31709.0 50-51 31902.0 52-53 31192.0 54-55 30326.0 56-57 29295.0 58-59 28114.0 60-61 26541.0 62-63 25800.0 64-65 23815.0 66-67 22560.0 68-69 21021.0 70-71 19865.0 72-73 18066.0 74-75 16678.0 76-77 15383.0 78-79 13865.0 80-81 12800.0 82-83 11596.0 84-85 10531.0 86-87 9490.0 88-89 8380.0 90-91 7554.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.85886778038666 #Duplication Level Percentage of deduplicated Percentage of total 1 99.86778024640009 99.72683463145977 2 0.12436139985727468 0.24837177170662778 3 0.006604776929019654 0.019786366382217653 4 0.0012535768136246296 0.005007230451372012 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0019278623565596258 0.0 0.0 0.0 0.0 2 0.0019278623565596258 0.0 0.0 0.0 1.4829710435074046E-4 3 0.0019278623565596258 0.0 0.0 0.0 1.4829710435074046E-4 4 0.0019278623565596258 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 5 0.0019278623565596258 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 6 0.0019278623565596258 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 7 0.0025210507739625875 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 8 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 9 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 10-11 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 12-13 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 2.965942087014809E-4 14-15 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 3.7074276087685113E-4 16-17 0.005190398652275915 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 18-19 0.005412844308802026 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 20-21 0.005709438517503508 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 22-23 0.005931884174029618 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 24-25 0.006080181278380358 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 26-27 0.006302626934906469 1.4829710435074046E-4 0.0 0.0 4.4489131305222135E-4 28-29 0.00667336969578332 4.4489131305222135E-4 0.0 0.0 4.4489131305222135E-4 30-31 0.007266558113186282 4.4489131305222135E-4 0.0 0.0 4.4489131305222135E-4 32-33 0.007711449426238503 4.4489131305222135E-4 0.0 0.0 4.4489131305222135E-4 34-35 0.008156340739290724 4.4489131305222135E-4 0.0 0.0 4.4489131305222135E-4 36-37 0.008897826261044427 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 38-39 0.009120271917570536 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 40-41 0.009268569021921278 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 42-43 0.009565163230622759 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 44-45 0.00963931178279813 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 46-47 0.00963931178279813 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 48-49 0.00963931178279813 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 50-51 0.00993590599149961 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 52-53 0.010306648752376461 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 54-55 0.010677391513253313 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 56-57 0.010751540065428684 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 58-59 0.011048134274130164 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 60-61 0.011270579930656274 4.4489131305222135E-4 0.0 0.0 5.931884174029618E-4 62-63 0.011567174139357756 5.931884174029618E-4 0.0 0.0 5.931884174029618E-4 64-65 0.011567174139357756 5.931884174029618E-4 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