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"TTCTC":108283, "TTCTG":86538, "TTCCA":82644, "TTCCT":118856, "TTCCC":74117, "TTCCG":69421, "TTCGA":60613, "TTCGT":51756, "TTCGC":61469, "TTCGG":66991, "TTGAA":72082, "TTGAT":70898, "TTGAC":49931, "TTGAG":63569, "TTGTA":32527, "TTGTT":77951, "TTGTC":56464, "TTGTG":54841, "TTGCA":42795, "TTGCT":69058, "TTGCC":77897, "TTGCG":46532, "TTGGA":67627, "TTGGT":81477, "TTGGC":80135, "TTGGG":77116, "TCAAA":66870, "TCAAT":50763, "TCAAC":68704, "TCAAG":67322, "TCATA":35417, "TCATT":51789, "TCATC":86159, "TCATG":50062, "TCACA":68177, "TCACT":30432, "TCACC":74057, "TCACG":40586, "TCAGA":77833, "TCAGT":32620, "TCAGC":65966, "TCAGG":57120, "TCTAA":22164, "TCTAT":35594, "TCTAC":36406, "TCTAG":28130, "TCTTA":29257, "TCTTT":98686, "TCTTC":128780, "TCTTG":79286, "TCTCA":51996, "TCTCT":73255, "TCTCC":108661, "TCTCG":60507, "TCTGA":244653, "TCTGT":41757, "TCTGC":73712, "TCTGG":78926, "TCCAA":70632, "TCCAT":62172, "TCCAC":66799, "TCCAG":175443, "TCCTA":33307, "TCCTT":97284, "TCCTC":131158, "TCCTG":113075, 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"CACGA":48516, "CACGT":322345, "CACGC":41017, "CACGG":41849, "CAGAA":80624, "CAGAT":223203, "CAGAC":44183, "CAGAG":56978, "CAGTA":27063, "CAGTT":48265, "CAGTC":97302, "CAGTG":27790, "CAGCA":91145, "CAGCT":54712, "CAGCC":98165, "CAGCG":54873, "CAGGA":100020, "CAGGT":50932, "CAGGC":69210, "CAGGG":50860, "CTAAA":24686, "CTAAT":20189, "CTAAC":21747, "CTAAG":20629, "CTATA":18878, "CTATT":34077, "CTATC":39836, "CTATG":32003, "CTACA":29758, "CTACT":26373, "CTACC":40575, "CTACG":29361, "CTAGA":67700, "CTAGT":13696, "CTAGC":20816, "CTAGG":21209, "CTTAA":21511, "CTTAT":32845, "CTTAC":20466, "CTTAG":32340, "CTTTA":38626, "CTTTT":96341, "CTTTC":85743, "CTTTG":70270, "CTTCA":88713, "CTTCT":121484, "CTTCC":95770, "CTTCG":79838, "CTTGA":69115, "CTTGT":57261, "CTTGC":61470, "CTTGG":73542, "CTCAA":63580, "CTCAT":50831, "CTCAC":41556, "CTCAG":59795, "CTCTA":30515, "CTCTT":75867, "CTCTC":61881, "CTCTG":54951, "CTCCA":184873, "CTCCT":130212, "CTCCC":77662, "CTCCG":68452, "CTCGA":59990, "CTCGT":46878, "CTCGC":54844, "CTCGG":57300, "CTGAA":244603, "CTGAT":53948, "CTGAC":39930, "CTGAG":54929, "CTGTA":23847, "CTGTT":66727, "CTGTC":40783, "CTGTG":40939, "CTGCA":61083, "CTGCT":105164, "CTGCC":82399, "CTGCG":53878, "CTGGA":80531, "CTGGT":73197, "CTGGC":77386, "CTGGG":75141, "CCAAA":102689, "CCAAT":53835, "CCAAC":101512, "CCAAG":87828, "CCATA":44773, "CCATT":56079, "CCATC":84681, "CCATG":56029, "CCACA":84403, "CCACT":42107, "CCACC":136305, "CCACG":56433, "CCAGA":138911, "CCAGT":124426, "CCAGC":109809, "CCAGG":70932, "CCTAA":27758, "CCTAT":33022, "CCTAC":33816, "CCTAG":39633, "CCTTA":30123, "CCTTT":77154, "CCTTC":99742, "CCTTG":75281, "CCTCA":74198, "CCTCT":68761, "CCTCC":131916, "CCTCG":70026, "CCTGA":66346, "CCTGT":61048, "CCTGC":110845, "CCTGG":75240, "CCCAA":98744, "CCCAT":59631, "CCCAC":89511, "CCCAG":96134, "CCCTA":33738, "CCCTT":56171, "CCCTC":69742, "CCCTG":63160, "CCCCA":100264, "CCCCT":58762, "CCCCC":70688, "CCCCG":66563, "CCCGA":64041, "CCCGT":40905, "CCCGC":77769, "CCCGG":62333, 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"CGGAC":32474, "CGGAG":70130, "CGGTA":33246, "CGGTT":56322, "CGGTC":51663, "CGGTG":61273, "CGGCA":70473, "CGGCT":69667, "CGGCC":70316, "CGGCG":87687, "CGGGA":54157, "CGGGT":47034, "CGGGC":53292, "CGGGG":54050, "GAAAA":103773, "GAAAT":67383, "GAAAC":67644, "GAAAG":80974, "GAATA":45042, "GAATT":52877, "GAATC":55654, "GAATG":48423, "GAACA":66431, "GAACT":193816, "GAACC":72280, "GAACG":46068, "GAAGA":609631, "GAAGT":47481, "GAAGC":85510, "GAAGG":77109, "GATAA":43958, "GATAT":41987, "GATAC":28630, "GATAG":52099, "GATTA":36121, "GATTT":72072, "GATTC":59505, "GATTG":55568, "GATCA":65442, "GATCT":66888, "GATCC":57475, "GATCG":624155, "GATGA":86646, "GATGT":46289, "GATGC":53956, "GATGG":75819, "GACAA":57685, "GACAT":38332, "GACAC":31156, "GACAG":49936, "GACTA":22797, "GACTT":45192, "GACTC":31019, "GACTG":36001, "GACCA":64364, "GACCT":48990, "GACCC":53613, "GACCG":51703, "GACGA":66221, "GACGT":28164, "GACGC":40345, "GACGG":46899, "GAGAA":94159, "GAGAT":185202, "GAGAC":52501, "GAGAG":59496, "GAGTA":23067, "GAGTT":43697, "GAGTC":27722, "GAGTG":25620, "GAGCA":453714, "GAGCT":43698, "GAGCC":57131, "GAGCG":41932, "GAGGA":107644, "GAGGT":50842, "GAGGC":63646, "GAGGG":48584, "GTAAA":24582, "GTAAT":23543, "GTAAC":19921, "GTAAG":21159, "GTATA":15360, "GTATT":31558, "GTATC":28429, "GTATG":33068, "GTACA":19006, "GTACT":19349, "GTACC":21932, "GTACG":17952, "GTAGA":83250, "GTAGT":22771, "GTAGC":27551, "GTAGG":25805, "GTTAA":17723, "GTTAT":27839, "GTTAC":21497, "GTTAG":31472, "GTTTA":27693, "GTTTT":78875, "GTTTC":69828, "GTTTG":69925, "GTTCA":51863, "GTTCT":63142, "GTTCC":73895, "GTTCG":47272, "GTTGA":64605, "GTTGT":60005, "GTTGC":55583, "GTTGG":82319, "GTCAA":46913, "GTCAT":41134, "GTCAC":71335, "GTCAG":44582, "GTCTA":24681, "GTCTT":59729, "GTCTC":54800, "GTCTG":260961, "GTCCA":46142, "GTCCT":48707, "GTCCC":38553, "GTCCG":31343, "GTCGA":50533, "GTCGT":41045, "GTCGC":45114, "GTCGG":47152, "GTGAA":50250, "GTGAT":45381, "GTGAC":28813, "GTGAG":40254, "GTGTA":16951, "GTGTT":42627, "GTGTC":26491, "GTGTG":33849, "GTGCA":31494, "GTGCT":43803, "GTGCC":41094, "GTGCG":32467, "GTGGA":57358, "GTGGT":74546, "GTGGC":58645, "GTGGG":63944, "GCAAA":61142, "GCAAT":44299, "GCAAC":57186, "GCAAG":63101, "GCATA":31143, "GCATT":41375, "GCATC":54248, "GCATG":38590, "GCACA":384581, "GCACT":25717, "GCACC":64107, "GCACG":32408, "GCAGA":114785, "GCAGT":33222, "GCAGC":72073, "GCAGG":88100, "GCTAA":20565, "GCTAT":30027, "GCTAC":28501, "GCTAG":33987, "GCTTA":28089, "GCTTT":68576, "GCTTC":97369, "GCTTG":66418, "GCTCA":52163, "GCTCT":49162, "GCTCC":75090, "GCTCG":52591, "GCTGA":65323, "GCTGT":47363, "GCTGC":76398, "GCTGG":99012, "GCCAA":82613, "GCCAT":60315, "GCCAC":67592, "GCCAG":95964, "GCCTA":34941, "GCCTT":69066, "GCCTC":76652, "GCCTG":75618, "GCCCA":77967, "GCCCT":57729, "GCCCC":67397, "GCCCG":58832, "GCCGA":79972, "GCCGT":53836, "GCCGC":90008, "GCCGG":78321, "GCGAA":56592, "GCGAT":52744, "GCGAC":45547, "GCGAG":54171, "GCGTA":21721, "GCGTT":39158, "GCGTC":37293, "GCGTG":34121, "GCGCA":45532, "GCGCT":45392, "GCGCC":66192, "GCGCG":47371, "GCGGA":61461, "GCGGT":60270, "GCGGC":81676, "GCGGG":63653, "GGAAA":83289, "GGAAT":59418, "GGAAC":65807, "GGAAG":568083, "GGATA":46767, "GGATT":65383, "GGATC":58513, "GGATG":67257, "GGACA":44182, "GGACT":32492, "GGACC":40901, "GGACG":39978, "GGAGA":142287, "GGAGT":34601, "GGAGC":61564, "GGAGG":98591, "GGTAA":27251, "GGTAT":31356, "GGTAC":21318, "GGTAG":51095, "GGTTA":31822, "GGTTT":87810, "GGTTC":66376, "GGTTG":94986, "GGTCA":48793, "GGTCT":58244, "GGTCC":40004, "GGTCG":51920, "GGTGA":64805, "GGTGT":47765, "GGTGC":53882, "GGTGG":103705, "GGCAA":69547, "GGCAT":51546, "GGCAC":40051, "GGCAG":86066, "GGCTA":36335, "GGCTT":79692, "GGCTC":57259, "GGCTG":87898, "GGCCA":68349, "GGCCT":57375, "GGCCC":48318, "GGCCG":66393, "GGCGA":80984, "GGCGT":45803, "GGCGC":62010, "GGCGG":103030, "GGGAA":58625, "GGGAT":53975, "GGGAC":34126, "GGGAG":70052, "GGGTA":28950, "GGGTT":77741, "GGGTC":44906, "GGGTG":60512, "GGGCA":57842, "GGGCT":58747, "GGGCC":44506, "GGGCG":60271, "GGGGA":58934, "GGGGT":57292, "GGGGC":53859, "GGGGG":78710 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1111542, "total_bases": 67804062, "q20_bases": 66271261, "q30_bases": 63539348, "total_cycles": 61, "quality_curves": { "A":[39.3442,39.1247,39.1362,38.9598,39.1141,38.9627,39.0817,38.8928,39.1383,39.1519,39.1116,39.0955,39.1651,39.1901,39.1451,39.1588,39.1477,39.1342,39.1633,39.1694,38.9561,39.1779,39.1639,39.1867,38.9972,39.177,39.1487,38.9359,39.1,38.9718,39.1211,39.1392,39.0776,39.1107,39.1027,39.104,39.1157,39.1036,39.0558,39.0191,38.9935,38.9229,38.8917,38.8221,38.6835,38.5277,38.2389,38.0353,38.1574,37.8691,37.6652,37.3951,37.2665,36.8164,36.7138,36.3916,36.1833,35.987,35.7997,35.3723,35.0957], 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"GC":[0.548099,0.527688,0.576962,0.579519,0.576802,0.527132,0.530463,0.508998,0.502954,0.514045,0.519225,0.515433,0.525837,0.527353,0.524414,0.528631,0.531234,0.525217,0.530175,0.528565,0.524166,0.529705,0.529142,0.523517,0.526339,0.527987,0.521723,0.52558,0.527373,0.523266,0.525258,0.52712,0.521848,0.526854,0.526899,0.520587,0.524962,0.524591,0.519395,0.524324,0.524904,0.520118,0.524473,0.525158,0.52122,0.524148,0.525583,0.520897,0.525671,0.527045,0.520966,0.526104,0.526252,0.520735,0.525114,0.528714,0.52227,0.526047,0.527427,0.525181,0.52743,0.528676,0.525215,0.531386,0.53121,0.526277,0.529355,0.530758,0.527115,0.531004,0.531946,0.528337,0.533175,0.532709,0.528442,0.536404,0.537749,0.535177,0.540768,0.541207,0.539948,0.545227,0.550065,0.549991,0.564578,0.566305,0.568351,0.573763,0.560952,0.540581,0.490822] }, "kmer_count": { "AAAAA":100593, "AAAAT":64469, "AAAAC":75110, "AAAAG":72044, "AAATA":40927, "AAATT":46735, "AAATC":61861, "AAATG":46775, "AAACA":59165, "AAACT":43369, 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"CTTGA":62313, "CTTGT":53482, "CTTGC":57435, "CTTGG":68927, "CTCAA":57086, "CTCAT":46955, "CTCAC":37923, "CTCAG":46679, "CTCTA":25517, "CTCTT":68594, "CTCTC":56966, "CTCTG":50765, "CTCCA":79373, "CTCCT":121512, "CTCCC":71881, "CTCCG":63575, "CTCGA":53563, "CTCGT":43515, "CTCGC":50337, "CTCGG":52760, "CTGAA":57910, "CTGAT":49877, "CTGAC":36832, "CTGAG":43910, "CTGTA":19023, "CTGTT":61360, "CTGTC":37702, "CTGTG":37612, "CTGCA":52941, "CTGCT":98262, "CTGCC":76617, "CTGCG":49706, "CTGGA":71129, "CTGGT":67844, "CTGGC":71507, "CTGGG":66095, "CCAAA":91070, "CCAAT":50097, "CCAAC":93366, "CCAAG":68516, "CCATA":36713, "CCATT":52204, "CCATC":77423, "CCATG":51677, "CCACA":72470, "CCACT":38813, "CCACC":123530, "CCACG":51363, "CCAGA":68708, "CCAGT":50133, "CCAGC":100625, "CCAGG":64820, "CCTAA":24176, "CCTAT":30554, "CCTAC":30016, "CCTAG":21011, "CCTTA":22899, "CCTTT":71839, "CCTTC":91518, "CCTTG":69956, "CCTCA":65021, "CCTCT":62648, "CCTCC":120829, "CCTCG":64650, "CCTGA":58910, "CCTGT":56465, "CCTGC":102842, "CCTGG":69627, "CCCAA":87091, "CCCAT":54969, "CCCAC":80498, "CCCAG":72189, "CCCTA":26648, "CCCTT":51484, "CCCTC":62402, "CCCTG":58104, "CCCCA":85264, "CCCCT":52714, "CCCCC":59086, "CCCCG":60453, "CCCGA":55727, "CCCGT":36871, "CCCGC":70457, "CCCGG":57347, "CCGAA":62611, "CCGAT":51655, "CCGAC":50615, "CCGAG":53174, "CCGTA":24843, "CCGTT":41371, "CCGTC":50120, "CCGTG":38205, "CCGCA":56230, "CCGCT":54574, "CCGCC":114672, "CCGCG":52833, "CCGGA":53355, "CCGGT":51754, "CCGGC":77105, "CCGGG":52892, "CGAAA":58077, "CGAAT":39552, "CGAAC":46841, "CGAAG":62559, "CGATA":32120, "CGATT":45652, "CGATC":55598, "CGATG":62206, "CGACA":41882, "CGACT":33938, "CGACC":57011, "CGACG":50943, "CGAGA":52787, "CGAGT":28982, "CGAGC":46937, "CGAGG":54477, "CGTAA":20128, "CGTAT":20606, "CGTAC":16630, "CGTAG":23662, "CGTTA":15328, "CGTTT":41244, "CGTTC":44139, "CGTTG":42473, "CGTCA":38789, "CGTCT":41383, "CGTCC":40789, "CGTCG":49423, "CGTGA":33386, "CGTGT":21330, "CGTGC":28791, "CGTGG":44902, "CGCAA":42204, "CGCAT":32127, "CGCAC":34947, "CGCAG":44484, "CGCTA":18802, "CGCTT":48047, "CGCTC":46620, "CGCTG":53261, "CGCCA":72181, "CGCCT":73991, "CGCCC":69203, "CGCCG":85670, "CGCGA":40677, "CGCGT":26986, "CGCGC":45692, "CGCGG":51149, "CGGAA":53008, "CGGAT":44884, "CGGAC":29306, "CGGAG":52124, "CGGTA":25641, "CGGTT":52457, "CGGTC":47259, "CGGTG":55994, "CGGCA":58596, "CGGCT":64566, "CGGCC":64692, "CGGCG":78887, "CGGGA":46569, "CGGGT":43753, "CGGGC":48622, "CGGGG":49025, "GAAAA":94212, "GAAAT":62451, "GAAAC":62942, "GAAAG":64226, "GAATA":39142, "GAATT":49212, "GAATC":51708, "GAATG":44980, "GAACA":58008, "GAACT":46288, "GAACC":66181, "GAACG":42001, "GAAGA":99530, "GAAGT":43680, "GAAGC":78312, "GAAGG":70882, "GATAA":39750, "GATAT":39411, "GATAC":26584, "GATAG":31485, "GATTA":30603, "GATTT":66908, "GATTC":55292, "GATTG":51295, "GATCA":57394, "GATCT":57903, "GATCC":52776, "GATCG":50210, "GATGA":77957, "GATGT":42883, "GATGC":49767, "GATGG":70530, "GACAA":51835, "GACAT":35191, "GACAC":28421, "GACAG":34073, "GACTA":18240, "GACTT":42313, "GACTC":28095, "GACTG":33208, "GACCA":55904, "GACCT":44796, "GACCC":49117, "GACCG":47271, "GACGA":58756, "GACGT":25648, "GACGC":36437, "GACGG":43629, "GAGAA":84350, "GAGAT":56420, "GAGAC":48316, "GAGAG":45633, "GAGTA":18674, "GAGTT":40578, "GAGTC":25700, "GAGTG":23465, "GAGCA":55323, "GAGCT":40298, "GAGCC":51932, "GAGCG":38246, "GAGGA":94623, "GAGGT":46678, "GAGGC":58665, "GAGGG":44511, "GTAAA":22090, "GTAAT":21726, "GTAAC":17998, "GTAAG":15838, "GTATA":12911, "GTATT":29222, "GTATC":26082, "GTATG":23379, "GTACA":16255, "GTACT":17808, "GTACC":20186, "GTACG":16398, "GTAGA":27946, "GTAGT":21081, "GTAGC":25148, "GTAGG":23688, "GTTAA":15801, "GTTAT":25928, "GTTAC":20074, "GTTAG":16561, "GTTTA":22148, "GTTTT":73195, "GTTTC":64340, "GTTTG":64899, "GTTCA":46047, "GTTCT":58791, "GTTCC":68260, "GTTCG":43719, "GTTGA":58090, "GTTGT":55718, "GTTGC":51626, "GTTGG":76714, "GTCAA":41872, "GTCAT":38124, "GTCAC":28139, "GTCAG":32388, "GTCTA":19329, "GTCTT":54783, "GTCTC":52235, "GTCTG":40747, "GTCCA":40163, "GTCCT":45071, "GTCCC":36026, "GTCCG":28946, "GTCGA":44092, "GTCGT":37546, "GTCGC":41672, "GTCGG":43022, "GTGAA":44177, "GTGAT":41773, "GTGAC":26275, "GTGAG":31458, "GTGTA":13358, "GTGTT":39154, "GTGTC":24291, "GTGTG":31082, "GTGCA":26719, "GTGCT":40024, "GTGCC":37832, "GTGCG":29433, "GTGGA":50242, "GTGGT":68432, "GTGGC":54247, "GTGGG":58550, "GCAAA":53363, "GCAAT":41160, "GCAAC":52368, "GCAAG":48224, "GCATA":25006, "GCATT":38137, "GCATC":49764, "GCATG":35503, "GCACA":33454, "GCACT":23507, "GCACC":57875, "GCACG":29074, "GCAGA":54172, "GCAGT":30852, "GCAGC":66081, "GCAGG":79528, "GCTAA":17731, "GCTAT":27448, "GCTAC":26209, "GCTAG":16861, "GCTTA":22188, "GCTTT":63769, "GCTTC":90831, "GCTTG":62018, "GCTCA":45047, "GCTCT":44615, "GCTCC":69894, "GCTCG":47781, "GCTGA":57594, "GCTGT":43554, "GCTGC":70575, "GCTGG":91182, "GCCAA":72419, "GCCAT":55676, "GCCAC":61593, "GCCAG":68025, "GCCTA":26970, "GCCTT":64209, "GCCTC":70969, "GCCTG":69502, "GCCCA":66587, "GCCCT":53068, "GCCCC":61528, "GCCCG":53207, "GCCGA":69263, "GCCGT":49282, "GCCGC":81651, "GCCGG":71240, "GCGAA":48275, "GCGAT":48106, "GCGAC":41363, "GCGAG":41656, "GCGTA":16834, "GCGTT":35926, "GCGTC":34046, "GCGTG":30607, "GCGCA":37608, "GCGCT":41199, "GCGCC":60414, "GCGCG":42420, "GCGGA":51805, "GCGGT":55605, "GCGGC":74250, "GCGGG":57855, "GGAAA":73042, "GGAAT":55313, "GGAAC":60675, "GGAAG":68475, "GGATA":37952, "GGATT":60892, "GGATC":50214, "GGATG":61846, "GGACA":37552, "GGACT":30032, "GGACC":37497, "GGACG":36041, "GGAGA":84234, "GGAGT":32217, "GGAGC":56483, "GGAGG":89153, "GGTAA":23518, "GGTAT":29122, "GGTAC":19675, "GGTAG":29058, "GGTTA":24603, "GGTTT":81451, "GGTTC":61910, "GGTTG":88266, "GGTCA":41615, "GGTCT":53959, "GGTCC":37141, "GGTCG":47421, "GGTGA":56645, "GGTGT":44039, "GGTGC":49232, "GGTGG":95284, "GGCAA":60423, "GGCAT":47634, "GGCAC":36674, "GGCAG":60309, "GGCTA":28142, "GGCTT":74076, "GGCTC":52757, "GGCTG":80986, "GGCCA":56913, "GGCCT":52997, "GGCCC":44218, "GGCCG":59931, "GGCGA":70370, "GGCGT":41678, "GGCGC":56029, "GGCGG":93431, "GGGAA":50715, "GGGAT":49863, "GGGAC":31239, "GGGAG":52211, "GGGTA":22211, "GGGTT":72480, "GGGTC":41620, "GGGTG":55254, "GGGCA":48490, "GGGCT":54790, "GGGCC":40726, "GGGCG":54301, "GGGGA":50432, "GGGGT":52558, "GGGGC":49573, "GGGGG":41705 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/AM-AL-18A_S99_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/AM-AL-18A_S99_R2_001.fastq.gz -o AM-AL-18A.trim.R1.fastq.gz -O AM-AL-18A.trim.R2.fastq.gz -m --merged_out AM-AL-18A.trim.merged.fastq.gz " }