##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-17C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739386 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.258749827559626 40.0 40.0 40.0 40.0 40.0 2 39.15656233685788 40.0 40.0 40.0 40.0 40.0 3 39.15068989675217 40.0 40.0 40.0 40.0 40.0 4 39.05717446638157 40.0 40.0 40.0 40.0 40.0 5 39.213217453400524 40.0 40.0 40.0 40.0 40.0 6 39.247261917320586 40.0 40.0 40.0 40.0 40.0 7 39.29653523328816 40.0 40.0 40.0 40.0 40.0 8 39.32030630820708 40.0 40.0 40.0 40.0 40.0 9 39.348086114695164 40.0 40.0 40.0 40.0 40.0 10-11 39.33383915843686 40.0 40.0 40.0 40.0 40.0 12-13 39.344055743549376 40.0 40.0 40.0 40.0 40.0 14-15 39.355930461220524 40.0 40.0 40.0 40.0 40.0 16-17 39.48668760295705 40.0 40.0 40.0 40.0 40.0 18-19 39.49877871639441 40.0 40.0 40.0 40.0 40.0 20-21 39.513536907650405 40.0 40.0 40.0 40.0 40.0 22-23 39.509011531189394 40.0 40.0 40.0 40.0 40.0 24-25 39.50382073774726 40.0 40.0 40.0 40.0 40.0 26-27 39.49157003243232 40.0 40.0 40.0 40.0 40.0 28-29 39.494431866440536 40.0 40.0 40.0 40.0 40.0 30-31 39.48337298930391 40.0 40.0 40.0 40.0 40.0 32-33 39.48541002846525 40.0 40.0 40.0 40.0 40.0 34-35 39.479300099284046 40.0 40.0 40.0 40.0 40.0 36-37 39.47226027793985 40.0 40.0 40.0 40.0 40.0 38-39 39.45752250594983 40.0 40.0 40.0 40.0 40.0 40-41 39.44287784619485 40.0 40.0 40.0 40.0 40.0 42-43 39.44470429805044 40.0 40.0 40.0 40.0 40.0 44-45 39.42173310906073 40.0 40.0 40.0 40.0 40.0 46-47 39.41311260341301 40.0 40.0 40.0 40.0 40.0 48-49 39.40852997698523 40.0 40.0 40.0 40.0 40.0 50-51 39.41525711247583 40.0 40.0 40.0 40.0 40.0 52-53 39.408730991860985 40.0 40.0 40.0 40.0 40.0 54-55 39.382055374880466 40.0 40.0 40.0 40.0 40.0 56-57 39.373720420355454 40.0 40.0 40.0 40.0 40.0 58-59 39.36084317761756 40.0 40.0 40.0 40.0 40.0 60-61 39.335188473272495 40.0 40.0 40.0 40.0 40.0 62-63 39.355479986690945 40.0 40.0 40.0 40.0 40.0 64-65 39.36009558542479 40.0 40.0 40.0 40.0 40.0 66-67 39.35718361258665 40.0 40.0 40.0 40.0 40.0 68-69 39.35478694255096 40.0 40.0 40.0 40.0 40.0 70-71 39.35053692833242 40.0 40.0 40.0 40.0 40.0 72-73 39.357407608404955 40.0 40.0 40.0 40.0 40.0 74-75 39.34815216018255 40.0 40.0 40.0 40.0 40.0 76-77 39.34194860484442 40.0 40.0 40.0 40.0 40.0 78-79 39.33197632664285 40.0 40.0 40.0 40.0 40.0 80-81 39.34567857816904 40.0 40.0 40.0 40.0 40.0 82-83 39.322878031000904 40.0 40.0 40.0 40.0 40.0 84-85 39.35541983044411 40.0 40.0 40.0 40.0 40.0 86-87 39.35065235978499 40.0 40.0 40.0 40.0 40.0 88-89 39.38264795278066 40.0 40.0 40.0 40.0 40.0 90-91 39.428292000156944 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24780957649699786 1101 2 0.09281437158085737 1101 3 0.38005260704495214 1101 4 0.1372878186157962 1101 5 0.11187360317629924 1101 6 0.13909167202723438 1101 7 0.04588099165205506 1101 8 -0.006153504788137809 1101 9 0.03646691647124101 1101 10-11 0.04121467868558426 1101 12-13 0.07625748692102263 1101 14-15 0.02606761534541846 1101 16-17 0.0936447734507837 1101 18-19 0.11952041616076059 1101 20-21 0.04407271838626059 1101 22-23 0.03765795918000947 1101 24-25 0.08056066523045047 1101 26-27 0.04635005413270932 1101 28-29 0.03843939537445351 1101 30-31 0.05522991475120165 1101 32-33 -0.007660377267669105 1101 34-35 0.04481076644427162 1101 36-37 0.055977075736564075 1101 38-39 0.03498414172256048 1101 40-41 0.052456665531742885 1101 42-43 0.06954577003568119 1101 44-45 0.02775495017589691 1101 46-47 0.012814203955777259 1101 48-49 0.008135688436134103 1101 50-51 -0.017869387997521358 1101 52-53 -0.0689199556632829 1101 54-55 0.06482726357601365 1101 56-57 0.06411424445008862 1101 58-59 0.29737235645709603 1101 60-61 -0.0565759837000428 1101 62-63 0.14073667650843902 1101 64-65 0.006974407454620746 1101 66-67 0.23992311115637932 1101 68-69 0.21897127144487172 1101 70-71 0.21044778228297645 1101 72-73 0.16093303709853757 1101 74-75 0.03749433094693444 1101 76-77 0.033134787384113906 1101 78-79 0.2830124558943865 1101 80-81 -0.11655979763838076 1101 82-83 0.2199334338693859 1101 84-85 0.5978332027466706 1101 86-87 0.35452692959829335 1101 88-89 0.5360078907271344 1101 90-91 0.5821057557226936 1102 1 0.37362502339445314 1102 2 0.19446626198327976 1102 3 0.1611657260713386 1102 4 -0.023929638608535697 1102 5 0.022797658368276075 1102 6 -0.12057423813245549 1102 7 0.21735297179728263 1102 8 -0.036920848221861036 1102 9 0.0800316528655145 1102 10-11 -0.08257251165377255 1102 12-13 -0.06542667691857673 1102 14-15 -0.09435068111410061 1102 16-17 0.10955060799122407 1102 18-19 -0.008250310321862742 1102 20-21 0.025093672528726074 1102 22-23 0.08268789703949153 1102 24-25 0.06501594088366858 1102 26-27 -0.0164246121627869 1102 28-29 0.020505304275907577 1102 30-31 0.027249536749351932 1102 32-33 -0.007855675976465193 1102 34-35 8.701114899878348E-5 1102 36-37 -0.03430182185734765 1102 38-39 -0.038749139020325174 1102 40-41 0.06775646618142872 1102 42-43 -0.07273942272738765 1102 44-45 -0.025598092281164497 1102 46-47 -0.06921746332209011 1102 48-49 -0.05052049787643398 1102 50-51 -0.10586290025568701 1102 52-53 -0.03605726638201645 1102 54-55 -0.02453423742455385 1102 56-57 0.09252306151566358 1102 58-59 -0.10216370533891705 1102 60-61 0.03916796164033087 1102 62-63 0.13440161686177987 1102 64-65 -0.09899398592661868 1102 66-67 -0.12012276167133251 1102 68-69 0.01680977412038942 1102 70-71 -0.03540159669523746 1102 72-73 0.13745115325021828 1102 74-75 0.08588511664945742 1102 76-77 0.20118910064191198 1102 78-79 0.15439218176380365 1102 80-81 0.30227490850538885 1102 82-83 0.36278511083799003 1102 84-85 0.22992199748606623 1102 86-87 0.18329463739826224 1102 88-89 -0.14160102517965356 1102 90-91 0.5821057557226936 1103 1 0.30602492489316546 1103 2 0.04742968073954046 1103 3 0.09013119100772116 1103 4 0.15004436092143436 1103 5 0.023632859407975104 1103 6 0.04827869313918143 1103 7 0.059849536268345105 1103 8 -0.03628789317384218 1103 9 0.07228638044360025 1103 10-11 0.04156345632893732 1103 12-13 -0.008129263888250193 1103 14-15 -0.0336169588733739 1103 16-17 -0.015387477581924713 1103 18-19 -0.005294023233673784 1103 20-21 -0.018014061851125973 1103 22-23 0.040051445757519843 1103 24-25 0.00935334961938139 1103 26-27 0.016503407302771222 1103 28-29 -0.015594981022019283 1103 30-31 0.10104185820562606 1103 32-33 -0.041122967944566824 1103 34-35 -0.04347408226470151 1103 36-37 -0.023459367453078528 1103 38-39 -0.03538001087509457 1103 40-41 -0.02740483658350712 1103 42-43 -0.01576094306883391 1103 44-45 0.15284241370651586 1103 46-47 -0.07853984660188473 1103 48-49 0.0776179530459089 1103 50-51 -0.032285607353777834 1103 52-53 0.1303446434477067 1103 54-55 0.02493857844848435 1103 56-57 0.056541394739511475 1103 58-59 -0.07249994890347722 1103 60-61 -0.01446669216292662 1103 62-63 -0.22364473190034317 1103 64-65 0.05280922441317415 1103 66-67 -0.0455919585692115 1103 68-69 0.04315395945653222 1103 70-71 0.00653881435403747 1103 72-73 0.31697769994968183 1103 74-75 0.01904751850443631 1103 76-77 0.16408074952165919 1103 78-79 -0.2516004308388844 1103 80-81 -0.31651780177516997 1103 82-83 -0.4469464935465979 1103 84-85 -0.3279272053666773 1103 86-87 -0.8694264510371141 1103 88-89 -0.5963229363405418 1103 90-91 -0.08456091094397777 1104 1 0.36656706371071124 1104 2 0.2747089074067546 1104 3 0.15558575560158516 1104 4 0.1450313473792164 1104 5 0.08277495687926972 1104 6 0.08200834421951697 1104 7 0.01413122704342129 1104 8 0.11646749521627697 1104 9 0.02064625890784555 1104 10-11 0.050412255664816996 1104 12-13 0.049874203465606115 1104 14-15 0.11732726491802481 1104 16-17 0.019199508758902084 1104 18-19 0.0696678396751409 1104 20-21 -0.008400327644352501 1104 22-23 0.10196555491688031 1104 24-25 0.0683931048870079 1104 26-27 0.11697011699214244 1104 28-29 0.09118945910325493 1104 30-31 -0.024118563198634035 1104 32-33 -0.03891158350366197 1104 34-35 0.04317868534203484 1104 36-37 -0.18715977888936663 1104 38-39 -0.011108892245395907 1104 40-41 0.011174583070193478 1104 42-43 0.0012212474560229225 1104 44-45 0.03938890792273497 1104 46-47 -0.0892890606922947 1104 48-49 -0.03561837216184216 1104 50-51 -0.06110300870066965 1104 52-53 0.05968236786747383 1104 54-55 -0.013605998380775475 1104 56-57 0.03649658994811489 1104 58-59 0.017496017968525734 1104 60-61 0.047202338198545135 1104 62-63 -0.029119734757017568 1104 64-65 0.10531620625820892 1104 66-67 0.0991985428442419 1104 68-69 0.1901132671216743 1104 70-71 -0.1914812580975962 1104 72-73 -0.12579845484209073 1104 74-75 -0.018187822725948877 1104 76-77 -0.04134414408629539 1104 78-79 -0.2131050475582228 1104 80-81 -0.050598772292865135 1104 82-83 -0.365359466005458 1104 84-85 -0.04779425861212161 1104 86-87 -0.2913695872659616 1104 88-89 -1.0115466324371454 1104 90-91 -1.1678942442773064 1201 1 0.3361988872051995 1201 2 0.23638305307274265 1201 3 0.1534770893780788 1201 4 0.12836386318152648 1201 5 0.14700019400555675 1201 6 0.1272567129632236 1201 7 0.06561312887993154 1201 8 -0.027251075649282086 1201 9 0.13980394260001816 1201 10-11 0.042241455955981166 1201 12-13 0.10164590595422851 1201 14-15 0.05924873345182391 1201 16-17 -0.05434445439469471 1201 18-19 -0.011463938996094214 1201 20-21 0.0014596603012932974 1201 22-23 -0.00924234434976512 1201 24-25 -0.014763895461996412 1201 26-27 0.07018930044216631 1201 28-29 0.010195814984093943 1201 30-31 0.050470712034744736 1201 32-33 0.032008075071289 1201 34-35 -0.050426064926092806 1201 36-37 0.030478214186359764 1201 38-39 -0.012899569146689771 1201 40-41 -0.03809759071311447 1201 42-43 0.06771756955538422 1201 44-45 -0.0049685532691015055 1201 46-47 0.07320411848017727 1201 48-49 0.07372003021797724 1201 50-51 0.05742957517153968 1201 52-53 -0.04130138565427188 1201 54-55 -0.045852532276548175 1201 56-57 0.11352460418675747 1201 58-59 -0.020237041087703744 1201 60-61 0.06000187316398353 1201 62-63 -0.06760527085409507 1201 64-65 -0.16487136535857871 1201 66-67 0.03163643918503567 1201 68-69 -0.03942736890794407 1201 70-71 0.08749797391866565 1201 72-73 -0.20495076520122524 1201 74-75 0.043701965263373665 1201 76-77 0.09382533677147364 1201 78-79 -0.14686123560787934 1201 80-81 -0.06588876654181064 1201 82-83 0.07666276324022903 1201 84-85 -0.1697534305688606 1201 86-87 -0.3092612043092231 1201 88-89 -0.23932788460864174 1201 90-91 -0.41789424427730637 1202 1 0.1294277203388745 1202 2 0.1575860500893569 1202 3 0.05541113721476876 1202 4 0.09539848274481955 1202 5 0.03305363588302157 1202 6 0.02806544335394534 1202 7 -0.02298506238911102 1202 8 -0.002737403718178655 1202 9 0.1894519570455344 1202 10-11 0.017419138399056067 1202 12-13 0.06628120003212246 1202 14-15 0.06427943186393037 1202 16-17 0.024971839221919367 1202 18-19 0.008122667360765945 1202 20-21 -0.05621651974725239 1202 22-23 0.06453635110430866 1202 24-25 0.0320646299272056 1202 26-27 -0.02976682456832691 1202 28-29 -0.032370809947821044 1202 30-31 -0.011992854545695764 1202 32-33 -0.07044223930762428 1202 34-35 -0.10288985034127762 1202 36-37 0.06306813191476124 1202 38-39 0.09771048389254844 1202 40-41 0.11086974551823658 1202 42-43 0.08398390249711696 1202 44-45 8.82879536121095E-4 1202 46-47 -0.08814144493980791 1202 48-49 -0.04624692725317203 1202 50-51 0.15106706045948215 1202 52-53 0.008308385570664711 1202 54-55 0.08287081799880269 1202 56-57 0.015257881539433527 1202 58-59 0.17714887082572517 1202 60-61 -0.006815526113015835 1202 62-63 -0.04119015754508126 1202 64-65 -0.15490019291339507 1202 66-67 -0.04363512431802263 1202 68-69 -0.21505634739480683 1202 70-71 0.058938209830991184 1202 72-73 -0.21982152726602777 1202 74-75 -0.2863494898179795 1202 76-77 -0.5917926195463039 1202 78-79 -0.14637069517436174 1202 80-81 -0.3339881788665835 1202 82-83 -0.3949367678418696 1202 84-85 -0.3605739814669633 1202 86-87 -0.6379808673391523 1202 88-89 -0.31298960300720324 1202 90-91 0.13766131127825076 1203 1 0.28148445637061315 1203 2 0.10541721950853855 1203 3 0.09165568169517968 1203 4 0.18620137881794108 1203 5 0.117063355317363 1203 6 -0.017507782375936642 1203 7 -0.014289708481783236 1203 8 0.037131485448163914 1203 9 0.0752993685121055 1203 10-11 -0.0036354284590984776 1203 12-13 0.0814969717488907 1203 14-15 -0.06473549441023607 1203 16-17 0.03533173544184365 1203 18-19 0.02484108228621551 1203 20-21 0.045406724573595625 1203 22-23 0.030636873983524993 1203 24-25 0.031875290297179504 1203 26-27 -0.02542298928831599 1203 28-29 0.03273183820014225 1203 30-31 0.062318285347480185 1203 32-33 0.03748309418454454 1203 34-35 -0.1030372093440235 1203 36-37 0.028643644823006298 1203 38-39 -0.03994168419554711 1203 40-41 -0.03622869167129039 1203 42-43 -0.010362444608269072 1203 44-45 -0.017734176096148246 1203 46-47 -0.0506225425933593 1203 48-49 -0.09603327207664591 1203 50-51 -0.04807008883100394 1203 52-53 -0.12718369352246128 1203 54-55 -0.0247557213610321 1203 56-57 -0.2795168891260218 1203 58-59 -0.044713855426479654 1203 60-61 -0.13251105867007595 1203 62-63 -0.2432376487682575 1203 64-65 -0.019012256073423828 1203 66-67 -0.05592922756141405 1203 68-69 -0.17786516895993998 1203 70-71 -0.14023666840147797 1203 72-73 0.05198084884006704 1203 74-75 -0.051033329799409444 1203 76-77 0.07768637199627193 1203 78-79 0.21293793653435245 1203 80-81 0.2836926786101017 1203 82-83 0.128797894578895 1203 84-85 0.036799641713109565 1203 86-87 -0.014959330855710107 1203 88-89 0.01680666184848434 1203 90-91 -0.145166971550033 1204 1 0.16246291167261973 1204 2 0.06100972146968786 1204 3 -0.011585064466103745 1204 4 -0.005402680603324939 1204 5 -0.03822290777487325 1204 6 -0.12159257177492577 1204 7 0.04626284843063644 1204 8 -0.23574321810246346 1204 9 -0.03836026402726844 1204 10-11 -0.09689856362179938 1204 12-13 -0.13408530490423232 1204 14-15 -0.1130421391692451 1204 16-17 -0.07637025797284736 1204 18-19 -0.08847094181741255 1204 20-21 -0.08149116935119594 1204 22-23 -0.09986407504474215 1204 24-25 -0.12794653436090186 1204 26-27 -0.13800805383394987 1204 28-29 -0.16001391719122893 1204 30-31 -0.19602347150614463 1204 32-33 -0.1367289954583626 1204 34-35 -0.03182346586527984 1204 36-37 -0.19786372920529516 1204 38-39 -0.06617470184820462 1204 40-41 -0.009086494716122218 1204 42-43 -0.1894422339590207 1204 44-45 -0.041285793698527584 1204 46-47 -0.12214016301234665 1204 48-49 -0.17791780458171758 1204 50-51 -0.10348316985883343 1204 52-53 -0.0725814823515023 1204 54-55 -0.0793130200808676 1204 56-57 -0.15967010339030452 1204 58-59 -0.08690425781580302 1204 60-61 -0.12823929559327496 1204 62-63 -0.14821788458645102 1204 64-65 -0.23579604390884867 1204 66-67 -0.11163829754573129 1204 68-69 -0.3665609935305554 1204 70-71 -0.3624013517424558 1204 72-73 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0.12592635984277223 2401 50-51 0.08046010464379094 2401 52-53 0.16960769901557882 2401 54-55 0.14058559289389194 2401 56-57 0.055390479764511724 2401 58-59 0.04847896623881809 2401 60-61 0.1307414633179178 2401 62-63 0.02421089632763085 2401 64-65 0.05495030407313095 2401 66-67 0.032325380929833614 2401 68-69 0.1703916185339196 2401 70-71 0.264522421758727 2401 72-73 -0.024441829858453445 2401 74-75 0.27448678835801843 2401 76-77 0.18538409718100013 2401 78-79 0.37682280284750647 2401 80-81 0.384025763372712 2401 82-83 0.5116194731969372 2401 84-85 0.1045397102428609 2401 86-87 0.47786118196480487 2401 88-89 0.3136635683828075 2401 90-91 -0.6968911094810721 2402 1 0.3213044817054538 2402 2 0.3202328752141952 2402 3 0.27628936261674397 2402 4 0.20162427783716197 2402 5 0.30226860175543635 2402 6 0.171487238949652 2402 7 0.10722958906202962 2402 8 0.10501444065090482 2402 9 0.08302235023894866 2402 10-11 0.06118788535955133 2402 12-13 0.04277394857814443 2402 14-15 0.12540590138447527 2402 16-17 -0.04398361123642758 2402 18-19 1.1961236098301242E-4 2402 20-21 0.04797804863429889 2402 22-23 0.018788962305897883 2402 24-25 0.0021942461990676065 2402 26-27 0.01091791026066602 2402 28-29 0.09019111055327755 2402 30-31 0.06007707100501136 2402 32-33 0.1366517727179115 2402 34-35 0.06379499457086268 2402 36-37 0.053042759739611256 2402 38-39 0.005229953803507215 2402 40-41 0.043478528886808476 2402 42-43 0.03645390388307845 2402 44-45 0.013634893918840874 2402 46-47 0.033264690630772975 2402 48-49 0.007816889981924646 2402 50-51 0.06143374686671166 2402 52-53 0.013344945466378988 2402 54-55 0.015953105290442693 2402 56-57 -0.013264168202198334 2402 58-59 0.007280736588619163 2402 60-61 0.08281399211720952 2402 62-63 0.050066229776057014 2402 64-65 0.11106496959013867 2402 66-67 0.10256065285535243 2402 68-69 0.024980891113273174 2402 70-71 -0.11166233424945204 2402 72-73 0.07484281629820799 2402 74-75 -0.06249184608577707 2402 76-77 -0.04702897515193882 2402 78-79 -0.13289932272959248 2402 80-81 -0.06912681504302043 2402 82-83 -0.01681968024222158 2402 84-85 -0.06717093415566922 2402 86-87 0.36274577528313046 2402 88-89 0.20016829172963924 2402 90-91 -0.3512275776106435 2403 1 -0.34330115161826313 2403 2 -0.17066700509154487 2403 3 -0.07091759248044127 2403 4 0.01165307249107883 2403 5 0.08752618640608034 2403 6 0.11888431683904344 2403 7 0.1422425001985772 2403 8 0.0843392718792586 2403 9 0.11793161614535563 2403 10-11 0.10502981770844144 2403 12-13 0.10671529359645149 2403 14-15 0.10642261543344489 2403 16-17 0.025300029003219038 2403 18-19 0.03838113941134935 2403 20-21 0.011528943350612053 2403 22-23 0.0454714451433631 2403 24-25 0.03608669304151135 2403 26-27 0.004543956900562307 2403 28-29 0.008872980651105422 2403 30-31 0.04905392136343778 2403 32-33 0.04973748638784059 2403 34-35 0.06251280398558379 2403 36-37 0.011407909974849417 2403 38-39 0.06856427608712323 2403 40-41 0.06496071400538739 2403 42-43 0.05737262033542834 2403 44-45 0.027335998337292722 2403 46-47 -0.035114219045965456 2403 48-49 0.08078501825433193 2403 50-51 0.05758639352402639 2403 52-53 0.02268850735928396 2403 54-55 -0.012840351799560779 2403 56-57 0.013804424604778376 2403 58-59 -0.033090030748866184 2403 60-61 -0.05580031153775877 2403 62-63 0.06592504958410927 2403 64-65 0.10419273025367204 2403 66-67 0.1525048333289476 2403 68-69 0.011947768207434706 2403 70-71 0.0904777337091005 2403 72-73 0.14998655393524984 2403 74-75 0.11709052169527467 2403 76-77 0.1653562915635831 2403 78-79 0.3015390762837029 2403 80-81 0.2734920038067017 2403 82-83 0.1325465827366088 2403 84-85 0.43654742969619065 2403 86-87 0.5353217936422823 2403 88-89 0.7264840812033242 2403 90-91 0.5821057557226936 2404 1 -0.4597534487130872 2404 2 -0.29187283663576835 2404 3 -0.3854002407608377 2404 4 -0.2950184372198734 2404 5 -0.13032994490960448 2404 6 -0.16884483590927601 2404 7 0.0012498058443952687 2404 8 -0.0037258670663860016 2404 9 -0.06399993693678852 2404 10-11 0.06268993545495505 2404 12-13 0.07812490055313503 2404 14-15 0.08087620544812779 2404 16-17 0.031833382227631546 2404 18-19 0.024469062285881193 2404 20-21 0.033708572727995545 2404 22-23 0.04232649326030469 2404 24-25 0.07047663161700513 2404 26-27 0.051018588213004534 2404 28-29 0.024900646541588856 2404 30-31 0.04225139000796929 2404 32-33 0.05715669323028294 2404 34-35 0.008608848758250076 2404 36-37 0.06536584251859523 2404 38-39 0.11099830513013842 2404 40-41 0.07126430078889001 2404 42-43 0.05930259600621213 2404 44-45 0.05418507852211718 2404 46-47 0.18167656443590374 2404 48-49 0.12733174314345064 2404 50-51 0.05202986622681749 2404 52-53 0.12422290389444157 2404 54-55 0.03679021650324188 2404 56-57 0.14944250487413768 2404 58-59 0.10391286342407824 2404 60-61 0.18756265610123535 2404 62-63 0.265319555929068 2404 64-65 0.24280092640827888 2404 66-67 0.19757892628089024 2404 68-69 0.32544850707040496 2404 70-71 0.3096976642892386 2404 72-73 0.18464713270542887 2404 74-75 0.2102647223291001 2404 76-77 0.4160863515856761 2404 78-79 0.29616090175483833 2404 80-81 0.19796853557635785 2404 82-83 0.27295598618140815 2404 84-85 0.11762934862977659 2404 86-87 0.43938870138705965 2404 88-89 0.7264840812033242 2404 90-91 0.5821057557226936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 12.0 31 432.0 32 2879.0 33 5395.0 34 7131.0 35 8872.0 36 12713.0 37 19401.0 38 51230.0 39 309383.0 40 321937.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.577988165680473 12.81149619611158 31.030803043110733 29.579712595097213 2 27.325489389846876 17.563120571212934 29.438457865742233 25.67293217319795 3 27.39834781385586 19.111855274120078 22.003181026367898 31.486615885656168 4 26.744082252030736 20.425731620560843 20.77886246155594 32.05132366585248 5 27.014576959801783 20.92628207729116 20.505392311999415 31.553748650907643 6 27.953328843121184 23.271471193666095 23.154617479909007 25.620582483303718 7 26.22202746603263 23.141768981289882 23.020857846916225 27.615345705761268 8 25.261906500799313 24.23808403188592 23.39454628570192 27.105463181612855 9 25.17223750517321 24.252961240813324 23.954335083434092 26.62046617057937 10-11 24.928521773471502 23.287565628778474 23.74754187934313 28.036370718406893 12-13 25.241213655654825 23.09517626787632 23.555693507856517 28.107916568612335 14-15 25.63363385295367 22.653390786409265 23.509438912827672 28.203536447809398 16-17 25.659060355484144 22.47986843137414 23.346601098749503 28.51447011439221 18-19 25.691857838801386 22.732037122693693 23.321647961957627 28.254457076547297 20-21 25.76401230209931 22.81514662165635 23.237456484163886 28.183384592080458 22-23 25.539163576264627 22.414611583124376 23.499768727024854 28.54645611358614 24-25 25.56506344453371 22.67557135244649 23.57631872932406 28.183046473695743 26-27 25.71775770707046 22.78045567538471 23.49571130640829 28.006075311136534 28-29 25.753801126880955 22.4685752773247 23.601880479208422 28.17574311658592 30-31 25.907765174952953 22.782530604847683 23.52192938856576 27.78777483163361 32-33 25.96034271929114 22.669133049740804 23.602469427991508 27.768054802976543 34-35 25.890532588902765 22.39877485705113 23.72682522722403 27.983867326822075 36-37 25.893264190714245 22.68984685407301 23.77212781435953 27.644761140853213 38-39 26.04217424441196 22.758927121206444 23.66233741894374 27.53656121543786 40-41 26.034446346351857 22.461700031308883 23.731805306403857 27.7720483159354 42-43 26.112366236367006 22.719004374911375 23.84209258039737 27.32653680832425 44-45 26.154168175549703 22.76883664886262 23.704081717709364 27.37291345787831 46-47 26.188229136100116 22.529661117764157 23.74148432481303 27.540625421322694 48-49 26.261050663946722 22.869086307739252 23.67578744315963 27.194075585154394 50-51 26.36126071429396 22.789329221137486 23.66113166952535 27.188278395043202 52-53 26.287630709270438 22.61709842998202 23.777535584060754 27.31773527668679 54-55 26.338075205701788 22.87326955558518 23.732358846723134 27.056296391989896 56-57 26.51235435887666 22.85170838209457 23.583504942650737 27.052432316378027 58-59 26.46011777301927 22.70041488222698 23.60830433618844 27.23116300856531 60-61 26.59027345888434 22.89114898909963 23.570979022281147 26.947598529734883 62-63 26.770579314401814 22.865719465055687 23.404961022326894 26.9587401982156 64-65 26.616472359799065 22.738256296836582 23.425761443713753 27.2195098996506 66-67 26.65801441507842 22.861438463531027 23.333840718457335 27.14670640293322 68-69 26.871864439922515 22.88706789614231 23.222240554363964 27.01882710957121 70-71 26.843154200250446 22.589463658971056 23.35808391682393 27.209298223954566 72-73 26.72606340313684 22.914635701387432 23.184059956151717 27.175240939324013 74-75 26.956521739130434 22.88480201863354 23.07210791925466 27.086568322981368 76-77 26.983951558019275 22.746441596817068 22.876737030078466 27.392869815085184 78-79 27.139476890815832 22.701124996457818 22.86406528946697 27.295332823259372 80-81 27.470899470899475 22.365079365079367 22.616402116402114 27.547619047619047 82-83 27.567285672176496 22.410040133324262 22.456522232047707 27.566151962451535 84-85 28.29876788857427 22.08617127114104 21.743322874416467 27.871737965868213 86-87 28.672191853288666 20.924440134015164 21.773055898430613 28.63031211426556 88-89 28.28286544758821 20.066963464439457 21.94709150447036 29.703079583501967 90-91 24.90258269143634 20.317172632532852 25.147258722247397 29.632985953783415 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 2.0 9 4.0 10 4.5 11 5.5 12 8.5 13 14.5 14 24.0 15 32.5 16 51.5 17 92.5 18 149.0 19 191.0 20 231.0 21 315.5 22 444.5 23 618.0 24 795.5 25 969.5 26 1245.5 27 1637.5 28 2059.5 29 2547.0 30 3124.5 31 3762.5 32 4585.0 33 5492.0 34 6641.5 35 8128.5 36 9739.0 37 11357.0 38 13273.0 39 15798.5 40 18558.5 41 21511.0 42 24724.0 43 28207.0 44 31914.5 45 35536.5 46 39445.5 47 43405.0 48 46683.5 49 49761.5 50 52737.5 51 55487.0 52 57500.5 53 58481.5 54 58803.5 55 58474.5 56 57676.0 57 56180.0 58 54039.0 59 51620.0 60 49130.0 61 46552.0 62 44017.0 63 41151.0 64 37774.0 65 34408.5 66 30888.5 67 27726.5 68 24656.0 69 21217.5 70 18000.5 71 15134.5 72 12515.5 73 9922.5 74 7621.0 75 6022.0 76 4729.0 77 3493.0 78 2599.0 79 1935.5 80 1350.0 81 932.0 82 660.5 83 453.5 84 304.0 85 210.5 86 132.0 87 82.5 88 56.0 89 37.5 90 26.0 91 17.0 92 8.5 93 3.5 94 3.5 95 3.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014877208927407334 2 0.006356625632619498 3 2.7049470777104243E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 2.3528525984904097E-4 66-67 2.6704408897909045E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10936.0 32-33 24227.0 34-35 26985.0 36-37 29063.0 38-39 31523.0 40-41 33393.0 42-43 34576.0 44-45 35349.0 46-47 35882.0 48-49 35383.0 50-51 35315.0 52-53 34510.0 54-55 33357.0 56-57 32165.0 58-59 30892.0 60-61 29044.0 62-63 27758.0 64-65 25738.0 66-67 24001.0 68-69 22315.0 70-71 20645.0 72-73 18884.0 74-75 17374.0 76-77 15840.0 78-79 14217.0 80-81 12926.0 82-83 11787.0 84-85 10283.0 86-87 9266.0 88-89 8329.0 90-91 7423.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83295166520722 #Duplication Level Percentage of deduplicated Percentage of total 1 99.84111178142335 99.6743288667539 2 0.15198502803943265 0.30346227916191687 3 0.005366746465891671 0.01607334421586355 4 0.001536444071317665 0.0061355098683260265 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014877208927407334 0.0 0.0 0.0 1.3524735388552122E-4 2 0.0014877208927407334 0.0 0.0 0.0 1.3524735388552122E-4 3 0.0014877208927407334 0.0 0.0 0.0 1.3524735388552122E-4 4 0.0014877208927407334 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 5 0.0016229682466262548 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 6 0.0018934629543972971 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 7 0.0020287103082828185 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 8 0.005409894155420849 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 9 0.005680388863191892 1.3524735388552122E-4 0.0 0.0 1.3524735388552122E-4 10-11 0.005680388863191892 1.3524735388552122E-4 0.0 0.0 2.0287103082828182E-4 12-13 0.005680388863191892 1.3524735388552122E-4 0.0 0.0 2.7049470777104243E-4 14-15 0.005680388863191892 1.3524735388552122E-4 0.0 0.0 2.7049470777104243E-4 16-17 0.0057480125401346525 1.3524735388552122E-4 0.0 0.0 4.057420616565637E-4 18-19 0.005815636217077413 1.3524735388552122E-4 0.0 0.0 4.057420616565637E-4 20-21 0.005815636217077413 1.3524735388552122E-4 0.0 0.0 4.057420616565637E-4 22-23 0.005815636217077413 1.3524735388552122E-4 0.0 0.0 5.409894155420849E-4 24-25 0.005815636217077413 1.3524735388552122E-4 0.0 0.0 5.409894155420849E-4 26-27 0.005950883570962934 1.3524735388552122E-4 0.0 0.0 8.114841233131274E-4 28-29 0.0062213782787339765 1.3524735388552122E-4 0.0 0.0 8.114841233131274E-4 30-31 0.006424249309562259 1.3524735388552122E-4 0.0 0.0 8.114841233131274E-4 32-33 0.006627120340390541 1.3524735388552122E-4 0.0 0.0 8.114841233131274E-4 34-35 0.007168109755932625 1.3524735388552122E-4 0.0 0.0 8.79107800255888E-4 36-37 0.007641475494531949 1.3524735388552122E-4 0.0 0.0 9.467314771986486E-4 38-39 0.007776722848417471 1.3524735388552122E-4 0.0 0.0 0.001014355154141409 40-41 0.007911970202302991 1.3524735388552122E-4 0.0 0.0 0.0010819788310841697 42-43 0.007979593879245753 1.3524735388552122E-4 0.0 0.0 0.0010819788310841697 44-45 0.008114841233131274 1.3524735388552122E-4 0.0 0.0 0.0010819788310841697 46-47 0.008385335940902317 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 48-49 0.008520583294787838 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 50-51 0.00872345432561612 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 52-53 0.008993949033387162 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 54-55 0.009129196387272682 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 56-57 0.009196820064215443 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 58-59 0.009332067418100965 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 60-61 0.009467314771986486 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 62-63 0.00987305683364305 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 64-65 0.010143551541414093 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 66-67 0.010278798895299613 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 68-69 0.010278798895299613 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 70-71 0.010346422572242372 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 72-73 0.010549293603070655 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 74-75 0.010549293603070655 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 76-77 0.010684540956956177 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 78-79 0.010684540956956177 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 80 0.010684540956956177 1.3524735388552122E-4 0.0 0.0 0.0012172261849696911 >>END_MODULE