##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-17B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 755576 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.28718487617394 40.0 40.0 40.0 40.0 40.0 2 39.17488115027476 40.0 40.0 40.0 40.0 40.0 3 39.15754867809459 40.0 40.0 40.0 40.0 40.0 4 39.069155187565514 40.0 40.0 40.0 40.0 40.0 5 39.190487786801064 40.0 40.0 40.0 40.0 40.0 6 39.24906031954429 40.0 40.0 40.0 40.0 40.0 7 39.29763782862346 40.0 40.0 40.0 40.0 40.0 8 39.30345061251284 40.0 40.0 40.0 40.0 40.0 9 39.32741643461412 40.0 40.0 40.0 40.0 40.0 10-11 39.322688915476405 40.0 40.0 40.0 40.0 40.0 12-13 39.32230245534532 40.0 40.0 40.0 40.0 40.0 14-15 39.32353330439294 40.0 40.0 40.0 40.0 40.0 16-17 39.466883543151184 40.0 40.0 40.0 40.0 40.0 18-19 39.475920357449155 40.0 40.0 40.0 40.0 40.0 20-21 39.485510392071745 40.0 40.0 40.0 40.0 40.0 22-23 39.48434571770411 40.0 40.0 40.0 40.0 40.0 24-25 39.48831884549006 40.0 40.0 40.0 40.0 40.0 26-27 39.47700562220081 40.0 40.0 40.0 40.0 40.0 28-29 39.47985642741432 40.0 40.0 40.0 40.0 40.0 30-31 39.47304524246401 40.0 40.0 40.0 40.0 40.0 32-33 39.47760170268857 40.0 40.0 40.0 40.0 40.0 34-35 39.4743468288268 40.0 40.0 40.0 40.0 40.0 36-37 39.468618246336035 40.0 40.0 40.0 40.0 40.0 38-39 39.45894238602302 40.0 40.0 40.0 40.0 40.0 40-41 39.44497058688455 40.0 40.0 40.0 40.0 40.0 42-43 39.45008729958873 40.0 40.0 40.0 40.0 40.0 44-45 39.434007136778426 40.0 40.0 40.0 40.0 40.0 46-47 39.429076691263845 40.0 40.0 40.0 40.0 40.0 48-49 39.4277611479686 40.0 40.0 40.0 40.0 40.0 50-51 39.42796949204468 40.0 40.0 40.0 40.0 40.0 52-53 39.42304491255699 40.0 40.0 40.0 40.0 40.0 54-55 39.405359941654446 40.0 40.0 40.0 40.0 40.0 56-57 39.396596603326714 40.0 40.0 40.0 40.0 40.0 58-59 39.39072214025513 40.0 40.0 40.0 40.0 40.0 60-61 39.36700042508428 40.0 40.0 40.0 40.0 40.0 62-63 39.38096088289414 40.0 40.0 40.0 40.0 40.0 64-65 39.370887068463816 40.0 40.0 40.0 40.0 40.0 66-67 39.36421476131679 40.0 40.0 40.0 40.0 40.0 68-69 39.36349289570318 40.0 40.0 40.0 40.0 40.0 70-71 39.361576773731194 40.0 40.0 40.0 40.0 40.0 72-73 39.353939733537516 40.0 40.0 40.0 40.0 40.0 74-75 39.35697207030279 40.0 40.0 40.0 40.0 40.0 76-77 39.34912403819684 40.0 40.0 40.0 40.0 40.0 78-79 39.34251700005778 40.0 40.0 40.0 40.0 40.0 80-81 39.33464707361175 40.0 40.0 40.0 40.0 40.0 82-83 39.33702367841509 40.0 40.0 40.0 40.0 40.0 84-85 39.34166816929802 40.0 40.0 40.0 40.0 40.0 86-87 39.330664627427936 40.0 40.0 40.0 40.0 40.0 88-89 39.344716325069555 40.0 40.0 40.0 40.0 40.0 90-91 39.442257468842016 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18141522764101836 1101 2 0.19884804523849908 1101 3 0.14702088076651165 1101 4 0.11947881909744495 1101 5 0.1761396125538539 1101 6 0.07274175416173279 1101 7 0.08355155564959205 1101 8 -0.01364193870539765 1101 9 0.06024663604360114 1101 10-11 -0.03131451748217273 1101 12-13 -0.024857022850149235 1101 14-15 0.04327698810404712 1101 16-17 0.044345602230322356 1101 18-19 -0.027678052514787055 1101 20-21 0.017231873354475624 1101 22-23 0.017723760883683326 1101 24-25 0.11497744685959077 1101 26-27 -0.023218959587403276 1101 28-29 0.017367475463416326 1101 30-31 0.11181866251501305 1101 32-33 0.12276164240296339 1101 34-35 0.03347637796654368 1101 36-37 0.08361171604415318 1101 38-39 -0.04066415349614516 1101 40-41 -0.011348666281570274 1101 42-43 0.037304156751112316 1101 44-45 -0.05456835826989703 1101 46-47 0.0019146348947316483 1101 48-49 -0.013076475996321335 1101 50-51 0.034034756850992665 1101 52-53 0.06627314660385508 1101 54-55 0.03691625543855537 1101 56-57 0.004583334446465415 1101 58-59 -0.05093135776365898 1101 60-61 0.025388565629768323 1101 62-63 -0.023839239162469994 1101 64-65 0.18874125251467433 1101 66-67 0.11560111720262256 1101 68-69 -0.1564914472755703 1101 70-71 -0.031163288649658227 1101 72-73 -0.3159677419488105 1101 74-75 -0.12213842281764897 1101 76-77 -0.015398665968120895 1101 78-79 -0.20884618505552055 1101 80-81 -0.017900829381446215 1101 82-83 -0.1458840299612234 1101 84-85 -0.09981328077606122 1101 86-87 -0.016776996461757676 1101 88-89 -0.367380938634831 1101 90-91 0.4475518251041919 1102 1 0.3773507848843991 1102 2 0.2969460274841964 1102 3 0.21746264880312793 1102 4 0.07034543673682236 1102 5 -0.022403232073834545 1102 6 0.1327833067997588 1102 7 0.1536332544050225 1102 8 0.0051009533421364495 1102 9 0.17582566040051972 1102 10-11 -0.061115072856892994 1102 12-13 -0.05367542496233568 1102 14-15 -0.02918695473494637 1102 16-17 0.08411879073221229 1102 18-19 -0.0373565218988503 1102 20-21 0.06841314975186208 1102 22-23 0.0657817577571862 1102 24-25 0.014936054966774748 1102 26-27 0.0064272803810894175 1102 28-29 0.0603025223407343 1102 30-31 0.0066639653389017894 1102 32-33 0.02586325709022219 1102 34-35 0.053091127070239 1102 36-37 0.013155157105359194 1102 38-39 -8.320588187871181E-4 1102 40-41 -0.030887572629232807 1102 42-43 0.09043437808507804 1102 44-45 0.0814966002427866 1102 46-47 -0.003366488033321957 1102 48-49 0.0662405972396698 1102 50-51 -0.03878245331691943 1102 52-53 0.0018396236501487806 1102 54-55 0.00909809289647967 1102 56-57 0.06443813013440547 1102 58-59 0.014054107605545596 1102 60-61 0.08616171315635057 1102 62-63 -0.21423343697086494 1102 64-65 -0.12089498851568692 1102 66-67 0.03632038884749278 1102 68-69 -0.039150574696051876 1102 70-71 0.04027958828346101 1102 72-73 4.5042756904223324E-4 1102 74-75 -0.20339036771766672 1102 76-77 0.03954851037789098 1102 78-79 0.013183247500229811 1102 80-81 -0.29962657932404113 1102 82-83 0.24185248073293764 1102 84-85 0.10573771569392676 1102 86-87 -0.0356338458270784 1102 88-89 -0.0582411536885985 1102 90-91 -0.3143529368005673 1103 1 0.30417079502959865 1103 2 0.2244785854912834 1103 3 0.11615688497963106 1103 4 0.059030494555180724 1103 5 -0.02375521879766751 1103 6 0.10930154365438227 1103 7 0.09648320745368011 1103 8 0.057935450194605664 1103 9 -0.20353270216978814 1103 10-11 -0.04776844216196707 1103 12-13 0.016933565060668343 1103 14-15 -0.05212622790964616 1103 16-17 0.03145858997384465 1103 18-19 -0.02972934601211108 1103 20-21 -0.06829803128960066 1103 22-23 0.04077424156924536 1103 24-25 -4.1312659651993044E-4 1103 26-27 -0.08762691828731306 1103 28-29 0.019054212655255753 1103 30-31 -0.0074736199714848794 1103 32-33 -0.016912403160034728 1103 34-35 -0.006470109048187567 1103 36-37 -0.021138452988225254 1103 38-39 -0.03496963285057575 1103 40-41 2.234084550778448E-4 1103 42-43 -0.11297720352335716 1103 44-45 -0.06157505618470793 1103 46-47 0.06803789727905496 1103 48-49 0.10022799759700973 1103 50-51 -0.05354015590064165 1103 52-53 0.04110598579814706 1103 54-55 0.020273329494237657 1103 56-57 -0.037277678025539274 1103 58-59 0.09061530398726347 1103 60-61 -0.006556030376103195 1103 62-63 -0.00863243262853075 1103 64-65 0.024905878206411103 1103 66-67 -0.2338220515987004 1103 68-69 -0.30537659611618295 1103 70-71 -0.02219271673873635 1103 72-73 -0.24434158812080398 1103 74-75 -0.06695950213474333 1103 76-77 -0.34283185663836946 1103 78-79 -0.14762495560999156 1103 80-81 -0.6078179537984454 1103 82-83 -0.0544822776045919 1103 84-85 -0.48437039033316864 1103 86-87 -0.3084787545415537 1103 88-89 -0.875626552669921 1103 90-91 0.4475518251041919 1104 1 0.2774877527628874 1104 2 0.10275404003979816 1104 3 0.1328084045615867 1104 4 0.12203442433659006 1104 5 0.022440756420337493 1104 6 0.037793145245558435 1104 7 -0.10741994893286488 1104 8 -0.10683927695714601 1104 9 0.06751214105723591 1104 10-11 -0.11310698274273534 1104 12-13 -0.13839756557834448 1104 14-15 0.019789833844875204 1104 16-17 0.028517405123899664 1104 18-19 0.020461419195818564 1104 20-21 -0.0013138154238276911 1104 22-23 -0.1061088820747429 1104 24-25 -0.20833438282121364 1104 26-27 -0.09494931861117095 1104 28-29 0.021238604432681996 1104 30-31 -0.042275741374872666 1104 32-33 -0.13924429953604545 1104 34-35 -0.021558424993727954 1104 36-37 -0.07433768056215939 1104 38-39 -0.025821345446701116 1104 40-41 -0.01650387258066388 1104 42-43 -0.06046144833561584 1104 44-45 0.01985535865858168 1104 46-47 -0.1301947753636341 1104 48-49 -0.041848355362780865 1104 50-51 -0.0513487011089353 1104 52-53 0.014856384867719896 1104 54-55 0.01663632721645314 1104 56-57 -0.023935658970998475 1104 58-59 -0.07535914791262854 1104 60-61 -0.3053531309835762 1104 62-63 -0.1526931412751793 1104 64-65 0.059627898909354826 1104 66-67 -0.051044171984024445 1104 68-69 0.07943357003852469 1104 70-71 -0.07273668681525436 1104 72-73 -0.012957533017790013 1104 74-75 -0.008642536133052658 1104 76-77 0.010268196058206058 1104 78-79 -0.23042245824469632 1104 80-81 -0.227530946100174 1104 82-83 0.399240798352686 1104 84-85 -0.5125586260456743 1104 86-87 -0.18774486360973697 1104 88-89 0.18261906136516615 1104 90-91 -0.6524481748958095 1201 1 0.35443214408036283 1201 2 0.17958084917439976 1201 3 0.027966772133915185 1201 4 0.20425264942610255 1201 5 0.07636186175239601 1201 6 0.19804724183114075 1201 7 0.029032933976367303 1201 8 0.07708704019276524 1201 9 0.02962190278463339 1201 10-11 0.09438339920835404 1201 12-13 0.13138011727373566 1201 14-15 0.07080809802922516 1201 16-17 0.042038363899933984 1201 18-19 0.0032001562986820886 1201 20-21 -0.03350751941886898 1201 22-23 0.01667321159757762 1201 24-25 -0.012878065966503982 1201 26-27 0.053648265794358 1201 28-29 0.046541595609305375 1201 30-31 0.06639004069672438 1201 32-33 -0.015662230272980082 1201 34-35 -0.011179860855186519 1201 36-37 -0.057806088741081396 1201 38-39 0.01453628151514863 1201 40-41 0.06737653292766055 1201 42-43 0.18027689490311616 1201 44-45 0.003404323827631117 1201 46-47 0.022627570776890593 1201 48-49 0.07851573303991444 1201 50-51 0.0016004077871301092 1201 52-53 0.05359593569257726 1201 54-55 0.04176592563069903 1201 56-57 -0.02491084486894124 1201 58-59 -0.08160337893522751 1201 60-61 0.0285383360646847 1201 62-63 0.12165239476326661 1201 64-65 -0.09872780968759542 1201 66-67 -0.2156523020749077 1201 68-69 -0.08467644350210435 1201 70-71 -0.029114055571575648 1201 72-73 -0.01124450318496173 1201 74-75 0.015918477339177173 1201 76-77 0.04168307068325561 1201 78-79 0.055492216269186656 1201 80-81 0.08643472615982262 1201 82-83 -0.10570280736460802 1201 84-85 -0.47170582717356524 1201 86-87 -0.5330816716331199 1201 88-89 -1.898681751642961 1201 90-91 -1.552448174895808 1202 1 0.19278987303350448 1202 2 0.12310523978484156 1202 3 0.09092450309080391 1202 4 0.17483764336476781 1202 5 0.17328310447629036 1202 6 0.028951031918602155 1202 7 -0.03424539650163183 1202 8 0.05232072718744263 1202 9 0.022528638380194366 1202 10-11 -0.05501597762632571 1202 12-13 0.04855523474340373 1202 14-15 -0.01341940740594083 1202 16-17 0.03640783481053944 1202 18-19 0.021083598335287945 1202 20-21 -0.03365780044438793 1202 22-23 0.010110940149964165 1202 24-25 0.02849930972603687 1202 26-27 0.09551655950359361 1202 28-29 -0.035982724869995764 1202 30-31 0.08864165120837697 1202 32-33 -0.021075376584519745 1202 34-35 -0.10566573118891398 1202 36-37 -3.9711226329330884E-4 1202 38-39 -6.662553100653668E-4 1202 40-41 0.04546855783400616 1202 42-43 0.07131864983799119 1202 44-45 -0.024212838220535104 1202 46-47 0.08305159028698483 1202 48-49 0.010595933433272364 1202 50-51 0.08152168922593006 1202 52-53 0.04154854421491194 1202 54-55 0.1605131701417548 1202 56-57 0.1324304120254567 1202 58-59 -0.0303226039139588 1202 60-61 0.19264778346185807 1202 62-63 -0.0438604045525679 1202 64-65 5.57350574922566E-4 1202 66-67 -0.05862946440901595 1202 68-69 -0.23383641250948983 1202 70-71 -0.3132215016060158 1202 72-73 -0.07499468392920505 1202 74-75 -0.02111103742723941 1202 76-77 0.07281987409002966 1202 78-79 -0.19633703628027632 1202 80-81 -0.13991269757036662 1202 82-83 -0.2306356536519445 1202 84-85 -0.4151096942410035 1202 86-87 0.3603618251685887 1202 88-89 0.3166654071762167 1202 90-91 0.4475518251041919 1203 1 0.1907434409165134 1203 2 0.14200951340137635 1203 3 -0.015038800098110983 1203 4 0.1531664031602773 1203 5 0.04094652635826179 1203 6 0.0388195938669611 1203 7 0.040433194677390816 1203 8 0.1902616738193501 1203 9 -0.12160681067840073 1203 10-11 -0.0011186666381348687 1203 12-13 -0.07001599394395441 1203 14-15 0.04975728344670216 1203 16-17 -0.010616319751250103 1203 18-19 0.01915138568529784 1203 20-21 0.046210161851760745 1203 22-23 -0.1270590250797241 1203 24-25 0.1275018882305048 1203 26-27 -0.022541633411179873 1203 28-29 -0.018874552371940467 1203 30-31 0.06851142197943005 1203 32-33 0.007675251003320227 1203 34-35 0.006586452782677554 1203 36-37 0.00809473654167192 1203 38-39 -0.0030958756724430714 1203 40-41 0.0372303661804807 1203 42-43 -0.025938091030994315 1203 44-45 0.04094756080474582 1203 46-47 0.07817751621898594 1203 48-49 0.06264787831528196 1203 50-51 -4.050496763952083E-4 1203 52-53 0.05535005651147884 1203 54-55 -0.05586482974280216 1203 56-57 -0.07639235496624508 1203 58-59 0.02562862388445808 1203 60-61 -0.056894206556975746 1203 62-63 -0.10648840636563506 1203 64-65 -0.05105011405682092 1203 66-67 0.008073360564942789 1203 68-69 -0.06303769335265486 1203 70-71 0.07280365744545492 1203 72-73 -0.13054153192986462 1203 74-75 -0.08717346043869156 1203 76-77 0.09515379747924158 1203 78-79 -0.1641850939342504 1203 80-81 -0.22468124873489614 1203 82-83 0.2982887784451549 1203 84-85 0.1978581338308345 1203 86-87 0.5020284918352544 1203 88-89 0.7159523946984976 1203 90-91 0.4475518251041919 1204 1 0.2480976827307515 1204 2 0.15649484614699105 1204 3 0.02065014144091748 1204 4 -0.11659631971748752 1204 5 -0.1494337611254366 1204 6 -0.020048920008960636 1204 7 -0.10850505741185401 1204 8 -0.04924030736909657 1204 9 -0.14692764350903076 1204 10-11 -0.09236543313529211 1204 12-13 0.008075415269544806 1204 14-15 -0.10035039706659887 1204 16-17 -0.006266350008189647 1204 18-19 -0.07591621031756546 1204 20-21 -0.0500363623998652 1204 22-23 -0.08008049183366239 1204 24-25 -0.1005214547388178 1204 26-27 -0.13058978992269488 1204 28-29 -0.14448598484101893 1204 30-31 -0.09769371153586093 1204 32-33 -0.176018125397718 1204 34-35 -0.11027726746188904 1204 36-37 -0.17752830834097466 1204 38-39 -0.050494038152884 1204 40-41 -0.09764580703273396 1204 42-43 -0.15374596579420796 1204 44-45 -0.018125367926010938 1204 46-47 -0.13130070070778288 1204 48-49 -0.14999814384817967 1204 50-51 -0.02597061062591166 1204 52-53 -0.07270289543323116 1204 54-55 -0.04460770334510755 1204 56-57 -0.11016194187969575 1204 58-59 -0.09607886759327755 1204 60-61 -0.028458152278226123 1204 62-63 0.0033784042984876805 1204 64-65 -0.11642444458712475 1204 66-67 -0.0881836691859732 1204 68-69 -0.005718282534154184 1204 70-71 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0.016863539260548066 2401 50-51 0.04509890828463625 2401 52-53 -0.02012022023367166 2401 54-55 0.19969246109847916 2401 56-57 0.07166209114664923 2401 58-59 -0.025952613185268092 2401 60-61 0.12203631336361553 2401 62-63 0.0711676395584604 2401 64-65 0.21107256949134978 2401 66-67 0.04128900089417442 2401 68-69 0.1627435458665758 2401 70-71 -0.005903004110045629 2401 72-73 0.08510399950276337 2401 74-75 0.13691576697053875 2401 76-77 0.12977381589976744 2401 78-79 0.14864965244031936 2401 80-81 0.05397208100016826 2401 82-83 0.3440442459883286 2401 84-85 0.5583175260389481 2401 86-87 0.13759907285676576 2401 88-89 0.5909523946984976 2401 90-91 0.4475518251041919 2402 1 0.33618146501670054 2402 2 0.3141891100027223 2402 3 0.315579907694989 2402 4 0.37789203187736575 2402 5 0.2472359486626985 2402 6 0.15834549870734094 2402 7 0.17885032027997028 2402 8 0.13901771401634733 2402 9 0.19356713920840463 2402 10-11 0.19704312955809655 2402 12-13 0.10187888013658153 2402 14-15 0.1392620592353282 2402 16-17 0.07467855764709697 2402 18-19 0.01578109710666098 2402 20-21 0.048254002348926406 2402 22-23 0.1320516723956544 2402 24-25 0.03436850842522432 2402 26-27 0.10976394528818645 2402 28-29 0.05979519421823909 2402 30-31 0.08679107837018307 2402 32-33 0.07235312066897137 2402 34-35 0.13829986774800318 2402 36-37 0.05521268191518658 2402 38-39 0.133224475089186 2402 40-41 0.138482921444826 2402 42-43 0.14040614241748983 2402 44-45 0.13865446592879493 2402 46-47 0.1151879208745612 2402 48-49 0.20490738092809835 2402 50-51 0.12033289358745236 2402 52-53 0.1556478371270984 2402 54-55 0.0433787825082419 2402 56-57 0.2053727153447369 2402 58-59 0.12963660754817852 2402 60-61 0.2564299695764376 2402 62-63 0.0821873130744919 2402 64-65 0.1927284973668293 2402 66-67 0.2088137939334942 2402 68-69 0.12386951447533079 2402 70-71 0.27648020530998707 2402 72-73 0.18485948434859978 2402 74-75 0.3524564182039782 2402 76-77 0.05999456011571169 2402 78-79 0.03566878966964282 2402 80-81 0.21208984374261775 2402 82-83 0.025921200417201362 2402 84-85 -0.23445695015604429 2402 86-87 0.03588130568806491 2402 88-89 -0.1631684844223784 2402 90-91 -0.05244817489580811 2403 1 -0.3173108237801756 2403 2 -0.20504684662085992 2403 3 0.02989944594567362 2403 4 -0.013385425056640088 2403 5 0.04540875638426911 2403 6 0.025481303994098425 2403 7 0.047974215531830566 2403 8 0.11421688480726999 2403 9 0.10776041713935314 2403 10-11 0.06265062033945412 2403 12-13 0.07860019713467636 2403 14-15 0.08340147606069337 2403 16-17 0.011441401526759876 2403 18-19 0.049597131408091855 2403 20-21 0.06608987389743959 2403 22-23 0.027066050461570512 2403 24-25 -0.010187956532554665 2403 26-27 0.06137222229033057 2403 28-29 0.05220652671062709 2403 30-31 0.040655087232849496 2403 32-33 0.050280282045996216 2403 34-35 0.03696085941141547 2403 36-37 0.03761317548325849 2403 38-39 0.1009830029142833 2403 40-41 0.06762286473321666 2403 42-43 0.08367912030321634 2403 44-45 0.07772983691170765 2403 46-47 0.0358849711498479 2403 48-49 0.10881109719539239 2403 50-51 0.09915903494485434 2403 52-53 0.0250944326736402 2403 54-55 -0.017694240439354303 2403 56-57 0.020665347507474507 2403 58-59 0.06704332732123675 2403 60-61 0.13620965598194346 2403 62-63 0.25253568003192584 2403 64-65 0.17141457332607501 2403 66-67 0.12004008733391203 2403 68-69 0.08436259314870398 2403 70-71 0.19275826011116948 2403 72-73 0.23670164261097426 2403 74-75 0.26161271281755916 2403 76-77 0.15180593633012052 2403 78-79 0.29537951831912324 2403 80-81 0.15941328629340035 2403 82-83 0.2357743993119641 2403 84-85 0.2750765184037931 2403 86-87 0.3464467308289656 2403 88-89 0.1442864204567016 2403 90-91 -0.567832790280427 2404 1 -0.4813716723657464 2404 2 -0.36016761714827084 2404 3 -0.31399471022658787 2404 4 -0.3132243851465333 2404 5 -0.22186200392678757 2404 6 -0.20894145292734123 2404 7 -0.21540892049293348 2404 8 -0.09444465933715662 2404 9 -0.03516514828143613 2404 10-11 0.09250448996461103 2404 12-13 0.06866975537180053 2404 14-15 -0.009455231807642406 2404 16-17 -0.03356301067051248 2404 18-19 -0.019488025997894454 2404 20-21 -0.02425424457188541 2404 22-23 0.02584878719012096 2404 24-25 0.06608556725063153 2404 26-27 0.05054029561674156 2404 28-29 0.07974743152966823 2404 30-31 -0.03524058411123576 2404 32-33 0.01026048615658226 2404 34-35 -0.07694952838376423 2404 36-37 0.024836030243797325 2404 38-39 0.027040660589683796 2404 40-41 -0.055499798439278436 2404 42-43 -0.031314660238756176 2404 44-45 -0.01700059747540905 2404 46-47 0.0037548325011798056 2404 48-49 -0.04410683779703817 2404 50-51 -0.05990634203951828 2404 52-53 -0.0414358055329771 2404 54-55 0.10985413343742323 2404 56-57 -0.026093038549717562 2404 58-59 -0.003792769342567226 2404 60-61 -0.03430140923573788 2404 62-63 0.11552340383911996 2404 64-65 0.05686274228622068 2404 66-67 0.09077087098201986 2404 68-69 0.05168288253750575 2404 70-71 -0.10792724355966499 2404 72-73 -0.09867009061547094 2404 74-75 0.049759713603435785 2404 76-77 0.17729150512276703 2404 78-79 -0.12087965742577467 2404 80-81 -0.07250591652562832 2404 82-83 -0.027472692964224166 2404 84-85 0.10309290116929049 2404 86-87 0.25800333460254876 2404 88-89 0.1392857280318296 2404 90-91 0.4475518251041919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 11.0 31 418.0 32 2950.0 33 5402.0 34 7203.0 35 9014.0 36 13227.0 37 20339.0 38 53224.0 39 317411.0 40 326376.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.769768319072483 13.624109110400825 30.518089112121395 29.0880334584053 2 27.576124965918147 17.993895708201087 29.011798277774396 25.41818104810637 3 27.340153791177524 19.64013923263232 21.923845573540508 31.095861402649653 4 26.667072538037207 21.157633381684963 20.748012112613424 31.427281967664406 5 26.89365464228615 21.47408070134573 20.74933560621301 30.882929050155113 6 27.539784217603525 23.84538418372209 23.23644477855305 25.37838682012134 7 26.0376189820746 23.788738657659852 23.201239848804093 26.972402511461453 8 25.03586667654875 24.88578250235582 23.69741759928849 26.38093322180694 9 24.846077694368272 24.930384236661833 24.190418965133883 26.033119103836018 10-11 24.680111596980318 24.015717809988672 23.99129935307633 27.312871239954685 12-13 24.971544887608925 23.702579224326872 23.931675966415025 27.394199921649182 14-15 25.308837231463148 23.310494245449828 23.720512562601247 27.660155960485778 16-17 25.37653392908192 23.154057301978888 23.622110813472105 27.847297955467088 18-19 25.408430124831916 23.41716782957638 23.581016337204993 27.593385708386712 20-21 25.387585100638454 23.54766429849545 23.564538841890162 27.500211758975933 22-23 25.12612894004045 23.05393501117029 23.913941152180588 27.905994896608682 24-25 25.199450485457454 23.313472106048895 23.98924793799697 27.497829470496683 26-27 25.3587329401675 23.38421283894671 23.906992810782768 27.35006141010302 28-29 25.395195188836066 23.146447213781272 23.876817156712228 27.581540440670427 30-31 25.487131264215236 23.355896827765473 23.833747037838247 27.32322487018105 32-33 25.56230832130278 23.29065112664902 23.87130166390828 27.275738888139923 34-35 25.483566085435445 22.988395255940773 24.034920182740162 27.493118475883616 36-37 25.584297244292838 23.26689515287012 23.990759305312377 27.15804829752467 38-39 25.713716227207016 23.3234742246548 23.931745337160898 27.03106421097729 40-41 25.612336470803548 23.0541450407047 24.024925309158277 27.308593179333474 42-43 25.691289108139685 23.311094310933946 24.03850804244845 26.95910853847792 44-45 25.816121924215707 23.33475785016968 23.890770212811997 26.95835001280262 46-47 25.657043630931298 23.13292187090912 24.037921023856093 27.17211347430349 48-49 25.862791787911522 23.370293651760694 23.85520421797845 26.911710342349338 50-51 25.89770145663504 23.39168782573834 23.878346474230476 26.832264243396143 52-53 25.72388475769139 23.225978514554928 24.014848175474928 27.035288552278757 54-55 25.933011963276503 23.395845332113076 23.947065986056433 26.72407671855399 56-57 26.06002831062196 23.390103378410487 23.696249778598265 26.853618532369296 58-59 25.98103768217913 23.270437261604236 23.773891078392687 26.97463397782395 60-61 26.082917260116155 23.41349218754116 23.84218077978824 26.661409772554446 62-63 26.108636393856795 23.36789246647399 23.594918986143757 26.92855215352546 64-65 26.18127232485263 23.283588631034558 23.562553415291564 26.97258562882125 66-67 26.295266965685375 23.32148137891473 23.59321916554988 26.790032489850013 68-69 26.39744736886533 23.515326479046028 23.35438015970827 26.732845992380373 70-71 26.360132247399402 23.22135311668414 23.419401661156357 26.9991129747601 72-73 26.42002835754449 23.397592975709035 23.405823397592975 26.7765552691535 74-75 26.606374155839024 23.33197302195367 23.000232569364908 27.061420252842396 76-77 26.60695331168593 23.11251941206921 23.171605306227026 27.10892197001783 78-79 26.717179722917923 23.030783375075295 23.094822940113495 27.15721396189329 80-81 26.969587369480436 23.21911561202667 22.67973958988552 27.131557428607373 82-83 27.170228815690216 22.68745285390998 22.494342469197885 27.647975861201914 84-85 27.74147188025381 22.293779489126308 22.007701068387657 27.957047562232223 86-87 28.479874951152794 21.34818288393904 21.725283313794453 28.446658851113714 88-89 27.513989332111656 21.253967734546286 21.84299224451062 29.389050688831443 90-91 24.95170637475853 20.396007726980038 25.402446877012235 29.249839021249198 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 1.5 8 3.5 9 3.0 10 3.5 11 6.5 12 8.5 13 11.0 14 18.0 15 36.5 16 64.5 17 101.0 18 144.5 19 194.0 20 261.0 21 373.0 22 516.5 23 671.0 24 890.0 25 1159.5 26 1497.0 27 1952.5 28 2480.5 29 3047.5 30 3701.5 31 4513.0 32 5475.0 33 6552.0 34 7923.0 35 9559.5 36 11497.5 37 13665.5 38 15956.0 39 18787.5 40 22037.0 41 25420.5 42 28769.0 43 32222.0 44 35837.0 45 39559.0 46 43673.0 47 47718.5 48 51000.5 49 53894.5 50 56536.5 51 58701.0 52 59858.0 53 60070.0 54 59856.5 55 58900.0 56 57186.5 57 54763.0 58 52112.5 59 49437.0 60 46518.0 61 43493.5 62 40664.5 63 37761.5 64 34525.5 65 31263.0 66 27751.5 67 24547.0 68 21659.0 69 18721.0 70 15913.0 71 13175.5 72 10700.5 73 8406.5 74 6475.0 75 5126.5 76 4014.0 77 2943.0 78 2176.5 79 1608.0 80 1105.5 81 766.5 82 539.5 83 349.0 84 224.5 85 161.5 86 112.5 87 73.5 88 48.0 89 30.0 90 19.0 91 14.5 92 11.5 93 7.0 94 4.0 95 2.5 96 1.5 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0014558429595434477 2 0.0055586731182568 3 7.940961597509715E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11442.0 32-33 24838.0 34-35 27379.0 36-37 29340.0 38-39 31403.0 40-41 33304.0 42-43 34498.0 44-45 35176.0 46-47 35201.0 48-49 35238.0 50-51 34861.0 52-53 34220.0 54-55 33403.0 56-57 32012.0 58-59 31136.0 60-61 29305.0 62-63 28105.0 64-65 26216.0 66-67 24934.0 68-69 23087.0 70-71 21690.0 72-73 20033.0 74-75 18764.0 76-77 17156.0 78-79 15602.0 80-81 14136.0 82-83 13271.0 84-85 11528.0 86-87 10570.0 88-89 9379.0 90-91 8349.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.8674180872528 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8749605623394 99.74254442927034 2 0.11793263087403205 0.23555254707253234 3 0.006495125489270503 0.01945954238298449 4 6.116812972987692E-4 0.0024434812741395747 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001588192319501943 0.0 0.0 0.0 0.0 2 0.001588192319501943 0.0 0.0 1.3234935995849525E-4 0.0 3 0.001588192319501943 0.0 0.0 1.3234935995849525E-4 0.0 4 0.001588192319501943 0.0 0.0 1.3234935995849525E-4 1.3234935995849525E-4 5 0.001588192319501943 0.0 0.0 1.3234935995849525E-4 1.3234935995849525E-4 6 0.001588192319501943 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 7 0.0017205416794604381 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 8 0.004632227598547333 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 9 0.004896926318464324 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 10-11 0.004896926318464324 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 12-13 0.004896926318464324 0.0 0.0 1.3234935995849525E-4 2.646987199169905E-4 14-15 0.004896926318464324 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 16-17 0.004896926318464324 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 18-19 0.004896926318464324 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 20-21 0.004896926318464324 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 22-23 0.005095450358402066 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 24-25 0.0055586731182568 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 26-27 0.006021895878111533 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 28-29 0.006352769278007771 1.3234935995849525E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 30-31 0.006815992037862505 2.646987199169905E-4 0.0 1.3234935995849525E-4 2.646987199169905E-4 32-33 0.007213040117737991 2.646987199169905E-4 0.0 1.985240399377429E-4 2.646987199169905E-4 34-35 0.008007136277488962 2.646987199169905E-4 0.0 2.646987199169905E-4 2.646987199169905E-4 36-37 0.0083380096773852 2.646987199169905E-4 0.0 2.646987199169905E-4 2.646987199169905E-4 38-39 0.008801232437239934 2.646987199169905E-4 0.0 2.646987199169905E-4 2.646987199169905E-4 40-41 0.009330629877073914 2.646987199169905E-4 0.0 2.646987199169905E-4 2.646987199169905E-4 42-43 0.009529153917011658 2.646987199169905E-4 0.0 2.646987199169905E-4 2.646987199169905E-4 44-45 0.009595328596990905 2.646987199169905E-4 1.3234935995849525E-4 2.646987199169905E-4 2.646987199169905E-4 46-47 0.00999237667686639 2.646987199169905E-4 1.3234935995849525E-4 2.646987199169905E-4 2.646987199169905E-4 48-49 0.010124726036824886 2.646987199169905E-4 1.3234935995849525E-4 2.646987199169905E-4 2.646987199169905E-4 50-51 0.010323250076762628 2.646987199169905E-4 1.3234935995849525E-4 2.646987199169905E-4 3.970480798754857E-4 52-53 0.010389424756741875 2.646987199169905E-4 1.3234935995849525E-4 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