##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-17A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 673330 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.285809335689784 40.0 40.0 40.0 40.0 40.0 2 39.177502858925045 40.0 40.0 40.0 40.0 40.0 3 39.16314734231358 40.0 40.0 40.0 40.0 40.0 4 39.07655978494943 40.0 40.0 40.0 40.0 40.0 5 39.19926336268991 40.0 40.0 40.0 40.0 40.0 6 39.238073455809186 40.0 40.0 40.0 40.0 40.0 7 39.27857662661696 40.0 40.0 40.0 40.0 40.0 8 39.31896098495537 40.0 40.0 40.0 40.0 40.0 9 39.329968960242375 40.0 40.0 40.0 40.0 40.0 10-11 39.32090059851781 40.0 40.0 40.0 40.0 40.0 12-13 39.32415606017851 40.0 40.0 40.0 40.0 40.0 14-15 39.34232545705672 40.0 40.0 40.0 40.0 40.0 16-17 39.47706770825599 40.0 40.0 40.0 40.0 40.0 18-19 39.487131124411505 40.0 40.0 40.0 40.0 40.0 20-21 39.50110050049753 40.0 40.0 40.0 40.0 40.0 22-23 39.49753612641647 40.0 40.0 40.0 40.0 40.0 24-25 39.5007856474537 40.0 40.0 40.0 40.0 40.0 26-27 39.490885598443555 40.0 40.0 40.0 40.0 40.0 28-29 39.48603804969332 40.0 40.0 40.0 40.0 40.0 30-31 39.482257110368685 40.0 40.0 40.0 40.0 40.0 32-33 39.47660386285696 40.0 40.0 40.0 40.0 40.0 34-35 39.482802301710464 40.0 40.0 40.0 40.0 40.0 36-37 39.47695814041202 40.0 40.0 40.0 40.0 40.0 38-39 39.46278965417197 40.0 40.0 40.0 40.0 40.0 40-41 39.45283446441262 40.0 40.0 40.0 40.0 40.0 42-43 39.44807453437619 40.0 40.0 40.0 40.0 40.0 44-45 39.43165248345958 40.0 40.0 40.0 40.0 40.0 46-47 39.42914912290254 40.0 40.0 40.0 40.0 40.0 48-49 39.429617391604125 40.0 40.0 40.0 40.0 40.0 50-51 39.42531563362569 40.0 40.0 40.0 40.0 40.0 52-53 39.420501113821715 40.0 40.0 40.0 40.0 40.0 54-55 39.39318336969207 40.0 40.0 40.0 40.0 40.0 56-57 39.392594968710306 40.0 40.0 40.0 40.0 40.0 58-59 39.38197440616826 40.0 40.0 40.0 40.0 40.0 60-61 39.337480514348556 40.0 40.0 40.0 40.0 40.0 62-63 39.3673875547098 40.0 40.0 40.0 40.0 40.0 64-65 39.347306817186855 40.0 40.0 40.0 40.0 40.0 66-67 39.35529373443748 40.0 40.0 40.0 40.0 40.0 68-69 39.3660242904803 40.0 40.0 40.0 40.0 40.0 70-71 39.363709526234494 40.0 40.0 40.0 40.0 40.0 72-73 39.34940311150351 40.0 40.0 40.0 40.0 40.0 74-75 39.35079322830774 40.0 40.0 40.0 40.0 40.0 76-77 39.31936881505443 40.0 40.0 40.0 40.0 40.0 78-79 39.32734671979005 40.0 40.0 40.0 40.0 40.0 80-81 39.34843220884545 40.0 40.0 40.0 40.0 40.0 82-83 39.31638710238137 40.0 40.0 40.0 40.0 40.0 84-85 39.31707414452849 40.0 40.0 40.0 40.0 40.0 86-87 39.334012758314664 40.0 40.0 40.0 40.0 40.0 88-89 39.34709665700405 40.0 40.0 40.0 40.0 40.0 90-91 39.48668551629868 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29658364495980294 1101 2 0.23056412359170508 1101 3 0.1665660395462396 1101 4 0.21084344578199676 1101 5 0.037741094372272244 1101 6 -0.02313276470692216 1101 7 0.19426431073425476 1101 8 0.1063297948413009 1101 9 -0.06161206086947857 1101 10-11 0.07788320009591132 1101 12-13 0.0779320452064951 1101 14-15 0.012373499507347674 1101 16-17 -0.057405738223550884 1101 18-19 0.06761347714584076 1101 20-21 -0.07172864125119816 1101 22-23 0.05394855156714584 1101 24-25 -0.059448327561661074 1101 26-27 0.009915439660851177 1101 28-29 0.14757000579718493 1101 30-31 0.026257405870396155 1101 32-33 0.03840806262902419 1101 34-35 -0.0010435786605853536 1101 36-37 0.050799363820551946 1101 38-39 0.044103541898998344 1101 40-41 0.00957723180757597 1101 42-43 0.03223263287493694 1101 44-45 -0.027367628239112207 1101 46-47 0.01705838419800898 1101 48-49 -0.13797460661269412 1101 50-51 -0.07047458898695425 1101 52-53 0.009862694140352346 1101 54-55 -0.05894077335513259 1101 56-57 0.12433651843343796 1101 58-59 0.06424727916651563 1101 60-61 -0.07963815998895996 1101 62-63 0.014296848053291455 1101 64-65 -0.0548233180938098 1101 66-67 0.09326380548503721 1101 68-69 0.14545047606737427 1101 70-71 0.16220367715085615 1101 72-73 -0.1672840220887295 1101 74-75 -0.12128233125008592 1101 76-77 -0.07663294235642581 1101 78-79 0.2536038920243655 1101 80-81 0.25365808262876754 1101 82-83 0.17958681766284457 1101 84-85 0.3467833496313091 1101 86-87 0.5411116068966564 1101 88-89 -0.28253168787443883 1101 90-91 -0.4213325374066983 1102 1 0.37158932445455406 1102 2 0.22241500976763717 1102 3 0.1464693658078744 1102 4 0.09119588901326381 1102 5 0.12396456239409304 1102 6 -0.09313070261344336 1102 7 0.10578442313582315 1102 8 0.04848073309383949 1102 9 0.10748939063462615 1102 10-11 -0.06402098595384587 1102 12-13 -0.043862185824863786 1102 14-15 -0.0894156287164165 1102 16-17 0.06700001187169136 1102 18-19 -0.01720287420693012 1102 20-21 -0.07511676568980619 1102 22-23 0.08037270672046049 1102 24-25 -0.09713920227375183 1102 26-27 -0.05604568812952948 1102 28-29 -0.08385350276748937 1102 30-31 -0.12493388999808275 1102 32-33 -0.011066797895594505 1102 34-35 -0.01260297512656905 1102 36-37 -0.010723244336809046 1102 38-39 -0.06421694901294472 1102 40-41 -0.07990412892696241 1102 42-43 -0.06534430601693941 1102 44-45 -0.009730204103320261 1102 46-47 -0.07765000231731278 1102 48-49 -0.14987032994148564 1102 50-51 -0.08941238020729969 1102 52-53 -0.11633553145109232 1102 54-55 -0.24770511393479921 1102 56-57 -0.2349878603370783 1102 58-59 -0.08329615682989555 1102 60-61 -0.07656065765372944 1102 62-63 -0.28627575667206173 1102 64-65 -0.06742111601360534 1102 66-67 -0.25296651597642494 1102 68-69 -0.1038255684174274 1102 70-71 -0.40067322220016877 1102 72-73 -0.35780984097765156 1102 74-75 -0.335458189236725 1102 76-77 -0.23575071884856413 1102 78-79 -0.31004088482796277 1102 80-81 -0.15946205077808173 1102 82-83 -0.4343041457426864 1102 84-85 -0.6120882665573788 1102 86-87 -0.42517021893100804 1102 88-89 -0.6812950618700029 1102 90-91 0.5198439331815337 1103 1 0.16622259815759577 1103 2 0.20107653905528622 1103 3 -0.054347110500550855 1103 4 0.1108096681977031 1103 5 0.090112904213278 1103 6 0.05543128995570612 1103 7 -0.07059280726645056 1103 8 0.0029231898618746754 1103 9 -0.06628245469357097 1103 10-11 -0.026885074757110772 1103 12-13 -0.024496101700286488 1103 14-15 0.07868675190832164 1103 16-17 0.05985355070018983 1103 18-19 -0.034440615594938606 1103 20-21 0.034887362561200064 1103 22-23 -0.0946284400159243 1103 24-25 0.009094456732711365 1103 26-27 -0.049633138246491626 1103 28-29 0.06534148828796305 1103 30-31 -0.062037692575941605 1103 32-33 -0.03109638049251373 1103 34-35 0.034606458893783554 1103 36-37 0.11685398413870729 1103 38-39 0.06553312012638912 1103 40-41 -0.07011656000054955 1103 42-43 -0.004771043659928864 1103 44-45 0.018069558815369646 1103 46-47 0.14648321133267928 1103 48-49 0.0112487735919089 1103 50-51 0.1234101449034739 1103 52-53 -0.05105085890743766 1103 54-55 0.05924160279685253 1103 56-57 -0.04910623768984834 1103 58-59 0.030580384040000297 1103 60-61 -0.044408129196341406 1103 62-63 -0.03715530369635189 1103 64-65 -0.08482056702207785 1103 66-67 -0.20535967314344816 1103 68-69 0.10222772747731312 1103 70-71 0.02507659264114892 1103 72-73 -0.05302235918301079 1103 74-75 -0.3093803686413352 1103 76-77 -0.16496105730703903 1103 78-79 -0.3357916355787083 1103 80-81 -0.33173527325639895 1103 82-83 0.48912500454402164 1103 84-85 -0.14366365505981804 1103 86-87 -0.31944906299464293 1103 88-89 -0.4336588426313597 1103 90-91 0.5198439331815337 1104 1 0.3581779911794314 1104 2 0.08343362094777973 1104 3 0.21035588529554872 1104 4 -0.15311766000647964 1104 5 0.13109739289198075 1104 6 0.1929965465453236 1104 7 0.12798351104695627 1104 8 0.09985591863474497 1104 9 -0.03719288945322319 1104 10-11 -0.03572876826147109 1104 12-13 -0.02414414083833094 1104 14-15 0.02857293994882326 1104 16-17 -0.020885594183830847 1104 18-19 -0.013651235066362233 1104 20-21 0.05522731614297527 1104 22-23 -0.06472733424965327 1104 24-25 -0.037289246937902476 1104 26-27 0.054131692335111836 1104 28-29 -0.11949481945307383 1104 30-31 0.05168627670328618 1104 32-33 0.04613706232203185 1104 34-35 0.06738151101916401 1104 36-37 0.006426819051277732 1104 38-39 0.02512590095130207 1104 40-41 -0.016409297654377042 1104 42-43 0.04431029971515699 1104 44-45 -0.019160510338217307 1104 46-47 -0.07291056138654994 1104 48-49 0.0446060572431648 1104 50-51 -0.0033043247859083635 1104 52-53 -0.07857217103236991 1104 54-55 -0.0453053117758202 1104 56-57 -0.07513043178235534 1104 58-59 -0.06079225960480272 1104 60-61 -0.2673156560822889 1104 62-63 -0.015499435273937934 1104 64-65 0.037978216947351484 1104 66-67 0.01450189058008533 1104 68-69 0.011072836669640651 1104 70-71 -0.0746105927517533 1104 72-73 0.08888705159024113 1104 74-75 0.21731416138811 1104 76-77 0.09339412086782772 1104 78-79 0.03111125427576411 1104 80-81 0.03226267005189243 1104 82-83 0.36321389620502487 1104 84-85 0.519417162394582 1104 86-87 0.5540426413794179 1104 88-89 0.23201496498310803 1104 90-91 0.5198439331815337 1201 1 0.2204851550762683 1201 2 0.2662400380518619 1201 3 0.12390205936708298 1201 4 0.17180409160506116 1201 5 0.06078811889746305 1201 6 0.14451551597771584 1201 7 -0.012827096803299298 1201 8 0.03812019684497869 1201 9 -0.050804812017247514 1201 10-11 0.06574669961236168 1201 12-13 0.0716489159416085 1201 14-15 0.09469915016602926 1201 16-17 -0.05019713000331194 1201 18-19 -0.09610701488381324 1201 20-21 -0.07961087022295033 1201 22-23 -0.014963984008652176 1201 24-25 -0.022635719726537218 1201 26-27 0.024193401168517426 1201 28-29 0.08975055814621413 1201 30-31 -0.013023812137205937 1201 32-33 0.1214766678245951 1201 34-35 -0.0022529277325418207 1201 36-37 -0.06552035271582923 1201 38-39 0.045776154766492994 1201 40-41 -0.06226470072388679 1201 42-43 -0.0902904770668087 1201 44-45 -0.032466218411386194 1201 46-47 0.08542931969427059 1201 48-49 -0.09029713616266832 1201 50-51 0.05025764229998231 1201 52-53 0.007529925751562416 1201 54-55 0.046346276018525145 1201 56-57 0.03174216193960433 1201 58-59 0.0483695427639006 1201 60-61 0.13922965224325168 1201 62-63 -0.13004365627435277 1201 64-65 -0.12032663827547196 1201 66-67 -0.1158687886930636 1201 68-69 -0.15714082164203091 1201 70-71 0.04384766199712686 1201 72-73 0.08814774513011514 1201 74-75 -0.01311195651155117 1201 76-77 -0.0019459726118427056 1201 78-79 -0.09273608002315115 1201 80-81 0.09905580610998754 1201 82-83 -0.08527306929225631 1201 84-85 -0.1388694480731374 1201 86-87 -0.4503691233264604 1201 88-89 -0.06678700823746908 1201 90-91 -0.013489400151797781 1202 1 0.11083342300552346 1202 2 0.1862399623873614 1202 3 0.08541909475631115 1202 4 0.218970818645694 1202 5 0.042791317614195634 1202 6 0.06489880711232843 1202 7 0.04725051605739594 1202 8 0.03281233218228152 1202 9 0.07421437996786295 1202 10-11 0.10928531388640295 1202 12-13 -0.05139606313558431 1202 14-15 -0.020742177216298785 1202 16-17 0.008629496460279995 1202 18-19 0.13182157170017916 1202 20-21 0.020630089682107666 1202 22-23 0.044435801041117884 1202 24-25 0.05364418469658716 1202 26-27 -0.025200384316946156 1202 28-29 0.07791284714866009 1202 30-31 0.008608037825140968 1202 32-33 0.0780706131213833 1202 34-35 0.019645378416015546 1202 36-37 0.0675154007780705 1202 38-39 0.06691979625674804 1202 40-41 -0.012510971047099417 1202 42-43 0.12120510353719283 1202 44-45 -0.04709966766576201 1202 46-47 -0.06839077926633763 1202 48-49 0.10443593150728958 1202 50-51 0.01036112837646641 1202 52-53 -0.04947859491105788 1202 54-55 -0.019237571303818868 1202 56-57 -0.008001321738291267 1202 58-59 0.07392925691035401 1202 60-61 0.03193682313489887 1202 62-63 -0.017800212405362004 1202 64-65 -0.3095299346547691 1202 66-67 -0.03781019651334816 1202 68-69 -0.01605182626650503 1202 70-71 -0.03564554483357085 1202 72-73 -0.14452878570999417 1202 74-75 -0.02563440750436996 1202 76-77 -0.16542043399593354 1202 78-79 0.08721900979720232 1202 80-81 -0.20123094254984153 1202 82-83 0.1825840735648967 1202 84-85 -0.6518651563342743 1202 86-87 0.1141680894080892 1202 88-89 0.36881387059186466 1202 90-91 0.5198439331815337 1203 1 0.21403266041324542 1203 2 0.1807310089867613 1203 3 -0.04586861912659401 1203 4 0.04916049376179643 1203 5 0.0431169073799893 1203 6 0.005170890778721571 1203 7 -0.04625225113765907 1203 8 0.02796791258101905 1203 9 0.10680006508313511 1203 10-11 -0.02257663350923167 1203 12-13 -0.006425884487285316 1203 14-15 -0.008192345130311196 1203 16-17 0.05750293679309948 1203 18-19 0.013589244324755612 1203 20-21 0.04851888805874438 1203 22-23 -0.027335757039459452 1203 24-25 -0.03966399173999946 1203 26-27 -0.025384164477713966 1203 28-29 -0.14255657443985115 1203 30-31 -0.021075955685958547 1203 32-33 -0.03217536582600644 1203 34-35 -0.09205772189960726 1203 36-37 -0.025277250516460015 1203 38-39 -0.0788280677472315 1203 40-41 -0.026158095198177023 1203 42-43 -0.09478812713605578 1203 44-45 0.015573297745916648 1203 46-47 -0.019930542548031838 1203 48-49 -0.0011080526304425575 1203 50-51 0.017861062527096294 1203 52-53 -0.05409288567521031 1203 54-55 -0.03303642529756701 1203 56-57 -0.0677590826989416 1203 58-59 0.025288774160820537 1203 60-61 -0.0782969628735799 1203 62-63 -0.1475548625937222 1203 64-65 -0.12825651106020786 1203 66-67 -0.16715258454073734 1203 68-69 -0.11899978258819033 1203 70-71 -0.20953703193924866 1203 72-73 -0.1355326459782873 1203 74-75 -0.056439011485331037 1203 76-77 0.12913288339347417 1203 78-79 0.32624186523612764 1203 80-81 -0.11450920154116062 1203 82-83 0.052312612352835686 1203 84-85 0.056462144779921175 1203 86-87 -0.39990472704163693 1203 88-89 -0.21114127862324494 1203 90-91 0.5198439331815337 1204 1 0.20700377150335214 1204 2 0.08771357227480792 1204 3 -0.09420987823808247 1204 4 -0.031651559723812284 1204 5 -0.25189473705352583 1204 6 0.04652738062017647 1204 7 -0.18182560161461936 1204 8 -0.07212442352239634 1204 9 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2401 50-51 0.14814014439311762 2401 52-53 0.08817500807898426 2401 54-55 0.14385332820575059 2401 56-57 0.21457705668139226 2401 58-59 0.10860145303545465 2401 60-61 0.14343432523483557 2401 62-63 0.10394652619039846 2401 64-65 0.3356301281271641 2401 66-67 0.152084658087972 2401 68-69 0.25510627072317504 2401 70-71 0.041658162082953254 2401 72-73 0.024399921471868424 2401 74-75 0.09844201599440794 2401 76-77 -0.0712397058248726 2401 78-79 -0.21470404264398013 2401 80-81 0.43125845959027487 2401 82-83 0.4338234305113957 2401 84-85 0.14303993857082986 2401 86-87 0.13254436353785337 2401 88-89 0.14358167244945008 2401 90-91 -0.48015606681846634 2402 1 0.2749363590992999 2402 2 0.30978119613558874 2402 3 0.3401095482912737 2402 4 0.2937253618891802 2402 5 0.1328719204500146 2402 6 0.21870813382903265 2402 7 0.12292160187018908 2402 8 0.18318419756829485 2402 9 0.21530817197073304 2402 10-11 0.11596907683128421 2402 12-13 0.12226997913289495 2402 14-15 0.13882412917688924 2402 16-17 0.10445427809870722 2402 18-19 0.017211507653136948 2402 20-21 0.06236008511647384 2402 22-23 0.08869738263175009 2402 24-25 0.035418727172015 2402 26-27 0.047915647070794876 2402 28-29 0.09629916898823154 2402 30-31 0.012366801090038848 2402 32-33 0.04686125483469539 2402 34-35 0.11040663263771933 2402 36-37 0.05140158959004992 2402 38-39 0.08257151370494142 2402 40-41 0.06785311568713581 2402 42-43 0.10542092458450725 2402 44-45 0.10945985563768801 2402 46-47 0.11380487453715915 2402 48-49 0.16914884656458895 2402 50-51 0.17350568335877625 2402 52-53 0.06602386454857623 2402 54-55 0.2079038033807592 2402 56-57 0.10813802432042507 2402 58-59 0.1312140160305546 2402 60-61 0.23320006931773207 2402 62-63 0.3317023664192078 2402 64-65 0.27896205772587024 2402 66-67 0.2795107973735469 2402 68-69 0.2723103269436322 2402 70-71 0.28052882326623774 2402 72-73 0.3487308223193111 2402 74-75 0.35205451481785843 2402 76-77 0.2526834703483374 2402 78-79 0.18330320261210176 2402 80-81 0.3202989601169506 2402 82-83 0.1882121670962107 2402 84-85 0.28368695719949955 2402 86-87 0.37524517302498595 2402 88-89 0.2785265928900884 2402 90-91 0.22354763688523605 2403 1 -0.14645137923637463 2403 2 -0.27293882577246364 2403 3 -0.08330924927602013 2403 4 -0.07080869258428635 2403 5 -0.002675039236883947 2403 6 -0.005011899489467453 2403 7 0.06334538580199478 2403 8 0.12363308447937271 2403 9 0.047111364613272144 2403 10-11 0.11066436089431875 2403 12-13 0.028741983605797827 2403 14-15 0.11591423746201457 2403 16-17 0.07369285909575041 2403 18-19 0.06049589017690238 2403 20-21 0.06113003913237236 2403 22-23 -0.011031048774356123 2403 24-25 0.014632319294250351 2403 26-27 -0.024060299710882305 2403 28-29 0.0049357349006626805 2403 30-31 0.03218218008797891 2403 32-33 -0.04034924089310721 2403 34-35 0.04797431215322234 2403 36-37 0.04529440940759599 2403 38-39 0.05060948627477302 2403 40-41 0.13378199287823378 2403 42-43 0.060264347985636846 2403 44-45 0.002665676846525855 2403 46-47 -0.03333337845626261 2403 48-49 0.03493999043203644 2403 50-51 0.04900227563582149 2403 52-53 0.05807243599090128 2403 54-55 -0.050474226878492345 2403 56-57 0.0344835456043171 2403 58-59 -0.11446562453741649 2403 60-61 0.08480976190553235 2403 62-63 0.15524678523376423 2403 64-65 0.2597248205303657 2403 66-67 -0.020184816250079507 2403 68-69 0.12787032011652855 2403 70-71 0.056067118275649364 2403 72-73 0.055522573187332114 2403 74-75 0.29208874087019154 2403 76-77 0.1876994400902987 2403 78-79 0.06055808332885704 2403 80-81 0.04184312660989775 2403 82-83 0.27049481909010353 2403 84-85 0.3951766907075793 2403 86-87 -0.007674150600529117 2403 88-89 0.6041079882389226 2403 90-91 0.5198439331815337 2404 1 -0.4686788268918818 2404 2 -0.2644405049028933 2404 3 -0.28532597213449407 2404 4 -0.2818403918289505 2404 5 -0.1409179247358665 2404 6 -0.016859649498847773 2404 7 -0.0782645922732641 2404 8 0.028460280872501187 2404 9 0.044225600680690036 2404 10-11 0.05244567430329283 2404 12-13 0.00921395062815833 2404 14-15 0.08573404833077092 2404 16-17 0.015538202461918615 2404 18-19 -0.02129244444807199 2404 20-21 -0.06362681262502434 2404 22-23 -0.041900111927773764 2404 24-25 0.04112305813372075 2404 26-27 -0.030406993225980727 2404 28-29 0.04347293740975289 2404 30-31 0.021086230093160907 2404 32-33 -0.02449208345444731 2404 34-35 0.013208507585552809 2404 36-37 -0.013062922627320006 2404 38-39 0.023147760234934367 2404 40-41 -0.025587851908206005 2404 42-43 -0.019485203844723742 2404 44-45 0.07651765231238983 2404 46-47 0.04011268526343059 2404 48-49 -0.01579288485915953 2404 50-51 -0.00411966837305755 2404 52-53 0.08243972727863991 2404 54-55 -0.011490862745105801 2404 56-57 0.02136140825117394 2404 58-59 0.04159717773904248 2404 60-61 -0.03100560562822352 2404 62-63 0.2446626040408333 2404 64-65 0.050516613292515444 2404 66-67 0.11468598102992189 2404 68-69 0.0130268341762374 2404 70-71 0.19222146734736612 2404 72-73 0.28208859703075007 2404 74-75 0.04975985455387644 2404 76-77 0.0823371296630313 2404 78-79 -0.06264348743056303 2404 80-81 -0.003161705157751271 2404 82-83 0.011534188642770005 2404 84-85 -0.6139736360898098 2404 86-87 0.047113594816224236 2404 88-89 0.06839370252463794 2404 90-91 0.19984393318153337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 20.0 31 362.0 32 2683.0 33 4797.0 34 6622.0 35 8098.0 36 11474.0 37 17741.0 38 47096.0 39 279081.0 40 295356.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.582408198121264 13.039616826940929 31.08097872498422 29.296996249953587 2 27.059525966676865 18.01463271180449 29.332705566430906 25.593135755087733 3 27.13221698288345 19.509597891063006 22.101956707384993 31.256228418668545 4 26.639983366254288 20.922281793474227 20.598072267684493 31.83966257258699 5 26.824439724949134 21.361145352204712 20.513715414432745 31.30069950841341 6 27.648107168847368 23.735761068124102 22.984717746127455 25.631414016901072 7 26.000029703117345 23.69937474937995 22.85595473244917 27.44464081505354 8 24.968291922237242 24.79541977930584 23.317392660359705 26.91889563809722 9 24.996509883712296 24.868043901207432 23.649770543418533 26.485675671661742 10-11 24.676236020970403 23.8406873301353 23.59563661206244 27.88744003683187 12-13 25.03237639790296 23.646800231684313 23.351699760889904 27.969123609522818 14-15 25.44339328412517 23.272837984346456 23.21536245228937 28.068406279239007 16-17 25.460918123357047 23.09974306803499 23.02593082143971 28.413407987168256 18-19 25.47406175278095 23.361130500646045 23.06573299867821 28.099074747894793 20-21 25.513641156639387 23.517740186832608 22.93095510373814 28.037663552789866 22-23 25.27750137376918 23.075906316367902 23.3090757875039 28.337516522359024 24-25 25.32517487710335 23.349249253709175 23.379100886638053 27.946474982549418 26-27 25.488616280278613 23.407838652666594 23.393581156342357 27.709963910712432 28-29 25.488096475725126 23.068777568205785 23.496131168966183 27.94699478710291 30-31 25.70357768482304 23.341499257789497 23.355162762790414 27.599760294597047 32-33 25.804703224293803 23.37316123295972 23.383514050905415 27.438621491841065 34-35 25.631579820737294 23.016759158509924 23.63895546247107 27.712705558281712 36-37 25.738427960210092 23.361595557260916 23.568714336992983 27.331262145536012 38-39 25.749239449803685 23.3293464035293 23.55018764571795 27.371226500949064 40-41 25.711738723748546 23.054147606731064 23.70323794670723 27.530875722813164 42-43 25.768836307252602 23.280562671034787 23.74417327312585 27.20642774858676 44-45 25.934844869230755 23.335244040980523 23.576886252666167 27.153024837122558 46-47 25.850252108339657 23.09691669269555 23.764780688054973 27.288050510909823 48-49 25.946013107690302 23.312894536532287 23.595652000834765 27.145440354942647 50-51 26.086147255942105 23.368239474743184 23.529334659845354 27.016278609469357 52-53 26.031579128322086 23.229654927398375 23.617875929724836 27.120890014554703 54-55 26.06693869849282 23.349922557413656 23.59915402819382 26.983984715899712 56-57 26.172904677726876 23.282535537040832 23.530331077068407 27.014228708163884 58-59 26.176541530386793 23.166710083263382 23.55900315493936 27.097745231410457 60-61 26.308568174120524 23.33191670208245 23.507089987724928 26.852425136072107 62-63 26.497984252426903 23.353321010833128 23.190799846696457 26.957894890043516 64-65 26.428310675241335 23.1469358738942 23.359106741434253 27.06564670943021 66-67 26.53521179592819 23.429309142969675 23.219248093977313 26.81623096712482 68-69 26.649031058531314 23.394579383567297 23.11245751122565 26.843932046675732 70-71 26.449610986263018 23.24071497110955 23.256499831766618 27.053174210860814 72-73 26.615187991116766 23.162002322857795 23.18629999854214 27.036509687483296 74-75 26.803530866327378 23.12257396704908 22.807441272031973 27.266453894591564 76-77 26.92918496423344 22.909934543044162 22.856768028281824 27.30411246444058 78-79 26.814669529783746 23.39076475660687 22.52675718174594 27.26780853186345 80-81 27.37764977446475 23.085068084115118 22.067512335613394 27.469769805806738 82-83 27.433544088653207 22.516826432796247 22.073560405194097 27.976069073356445 84-85 28.027263233111636 22.34742300152073 21.68599644551934 27.939317319848296 86-87 28.385166185743294 21.76979801218339 21.3797157208507 28.46532008122261 88-89 28.200098087297693 20.290695082259575 21.11997859913505 30.38922823130768 90-91 25.380434782608695 20.641304347826086 24.782608695652176 29.195652173913047 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 0.5 9 2.0 10 3.5 11 6.0 12 10.0 13 16.5 14 24.5 15 31.5 16 42.0 17 67.0 18 104.0 19 146.5 20 212.0 21 300.0 22 409.0 23 566.0 24 778.0 25 984.5 26 1220.0 27 1587.0 28 2015.0 29 2486.5 30 3097.5 31 3798.0 32 4587.0 33 5477.5 34 6532.5 35 7819.5 36 9475.0 37 11216.5 38 13207.0 39 15828.5 40 18571.0 41 21431.5 42 24436.5 43 27543.5 44 30885.0 45 34429.5 46 38111.5 47 41740.5 48 44918.5 49 47706.5 50 50113.5 51 52174.5 52 53707.0 53 54378.5 54 54607.0 55 54085.5 56 52690.0 57 51005.0 58 48929.0 59 46486.0 60 43991.5 61 41162.5 62 38643.0 63 36189.0 64 33012.5 65 29866.0 66 26603.5 67 23611.5 68 20959.0 69 18210.5 70 15558.0 71 12978.5 72 10589.5 73 8336.5 74 6402.0 75 5042.0 76 3943.0 77 2940.0 78 2217.5 79 1653.5 80 1168.0 81 811.5 82 550.5 83 345.5 84 244.0 85 177.0 86 110.5 87 66.5 88 48.0 89 34.5 90 20.5 91 12.0 92 8.0 93 6.0 94 4.0 95 2.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.425779335541265E-4 2 0.006534685815276314 3 7.425779335541265E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.750563555228412E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 10754.0 32-33 23506.0 34-35 26252.0 36-37 28435.0 38-39 30303.0 40-41 31693.0 42-43 32586.0 44-45 33228.0 46-47 33845.0 48-49 33143.0 50-51 32478.0 52-53 31552.0 54-55 30133.0 56-57 29251.0 58-59 27769.0 60-61 26055.0 62-63 24469.0 64-65 22486.0 66-67 21130.0 68-69 19390.0 70-71 17818.0 72-73 16290.0 74-75 14889.0 76-77 13574.0 78-79 12240.0 80-81 10836.0 82-83 9690.0 84-85 8829.0 86-87 7735.0 88-89 6764.0 90-91 6207.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.84720491928749 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8556979366195 99.7031233423612 2 0.13682939969473165 0.2732406622060593 3 0.006218489406237527 0.018626963580990506 4 0.001254174279538895 0.0050090318517447925 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016336714538190784 0.0 0.0 0.0 0.0 2 0.0016336714538190784 0.0 0.0 0.0 0.0 3 0.0016336714538190784 0.0 0.0 0.0 0.0 4 0.0016336714538190784 0.0 0.0 0.0 0.0 5 0.0016336714538190784 0.0 0.0 0.0 0.0 6 0.0019307026272407288 0.0 0.0 0.0 0.0 7 0.0020792182139515543 0.0 0.0 0.0 0.0 8 0.004901014361457235 0.0 0.0 0.0 1.485155867108253E-4 9 0.00504952994816806 0.0 0.0 0.0 1.485155867108253E-4 10-11 0.00504952994816806 0.0 0.0 0.0 1.485155867108253E-4 12-13 0.005272303328234298 0.0 1.485155867108253E-4 0.0 1.485155867108253E-4 14-15 0.005346561121589711 0.0 2.970311734216506E-4 1.485155867108253E-4 1.485155867108253E-4 16-17 0.005346561121589711 0.0 2.970311734216506E-4 1.485155867108253E-4 1.485155867108253E-4 18-19 0.005346561121589711 0.0 2.970311734216506E-4 2.2277338006623792E-4 2.2277338006623792E-4 20-21 0.005346561121589711 0.0 2.970311734216506E-4 2.970311734216506E-4 3.7128896677706324E-4 22-23 0.005643592295011362 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 24-25 0.006014881261788425 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 26-27 0.006460428021920901 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 28-29 0.0069802325754087895 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 30-31 0.007203005955475027 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 32-33 0.007425779335541265 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 34-35 0.007871326095673741 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 36-37 0.008316872855806217 0.0 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 38-39 0.008465388442517041 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 40-41 0.009059450789360343 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 42-43 0.009282224169426583 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 44-45 0.00957925534284823 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 46-47 0.01009905989633612 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 4.455467601324759E-4 48-49 0.010470348863113182 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 5.940623468433012E-4 50-51 0.010544606656468596 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 5.940623468433012E-4 52-53 0.010544606656468596 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 5.940623468433012E-4 54-55 0.010841637829890246 1.485155867108253E-4 2.970311734216506E-4 2.970311734216506E-4 5.940623468433012E-4 56-57 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