##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-16C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 578264 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.24669701036205 40.0 40.0 40.0 40.0 40.0 2 39.1515051948591 40.0 40.0 40.0 40.0 40.0 3 39.13956946999986 40.0 40.0 40.0 40.0 40.0 4 39.035271087254266 40.0 40.0 40.0 40.0 40.0 5 39.18226968996859 40.0 40.0 40.0 40.0 40.0 6 39.24574934631933 40.0 40.0 40.0 40.0 40.0 7 39.27584632624545 40.0 40.0 40.0 40.0 40.0 8 39.28425079202579 40.0 40.0 40.0 40.0 40.0 9 39.31372521893115 40.0 40.0 40.0 40.0 40.0 10-11 39.28860520454325 40.0 40.0 40.0 40.0 40.0 12-13 39.29312908982749 40.0 40.0 40.0 40.0 40.0 14-15 39.29790891357581 40.0 40.0 40.0 40.0 40.0 16-17 39.44708299323493 40.0 40.0 40.0 40.0 40.0 18-19 39.456120387919704 40.0 40.0 40.0 40.0 40.0 20-21 39.46925971528575 40.0 40.0 40.0 40.0 40.0 22-23 39.47467938519431 40.0 40.0 40.0 40.0 40.0 24-25 39.477408242601996 40.0 40.0 40.0 40.0 40.0 26-27 39.45920894262828 40.0 40.0 40.0 40.0 40.0 28-29 39.42522446495026 40.0 40.0 40.0 40.0 40.0 30-31 39.30541659334182 40.0 40.0 40.0 40.0 40.0 32-33 39.25878222116974 40.0 40.0 40.0 40.0 40.0 34-35 39.22082661652614 40.0 40.0 40.0 40.0 40.0 36-37 39.19516976122509 40.0 40.0 40.0 40.0 40.0 38-39 39.15110528465006 40.0 40.0 40.0 40.0 40.0 40-41 39.09845579519222 40.0 40.0 40.0 40.0 40.0 42-43 39.06264891224189 40.0 40.0 40.0 40.0 40.0 44-45 39.04880409025636 40.0 40.0 40.0 40.0 40.0 46-47 38.98543940224927 40.0 40.0 40.0 40.0 40.0 48-49 38.94468696993503 40.0 40.0 40.0 40.0 40.0 50-51 38.90320114011847 40.0 40.0 40.0 40.0 40.0 52-53 38.84610268681199 40.0 40.0 40.0 40.0 40.0 54-55 38.81543952887907 40.0 40.0 40.0 40.0 40.0 56-57 38.773677046459085 40.0 40.0 40.0 40.0 40.0 58-59 38.73023598289606 40.0 40.0 40.0 40.0 40.0 60-61 38.671146934473825 40.0 40.0 40.0 40.0 40.0 62-63 39.352446953943875 40.0 40.0 40.0 40.0 40.0 64-65 39.35035536943416 40.0 40.0 40.0 40.0 40.0 66-67 39.33919974348433 40.0 40.0 40.0 40.0 40.0 68-69 39.3345058883716 40.0 40.0 40.0 40.0 40.0 70-71 39.33770973810029 40.0 40.0 40.0 40.0 40.0 72-73 39.313610174944415 40.0 40.0 40.0 40.0 40.0 74-75 39.33227758492983 40.0 40.0 40.0 40.0 40.0 76-77 39.30439902107818 40.0 40.0 40.0 40.0 40.0 78-79 39.30832011749165 40.0 40.0 40.0 40.0 40.0 80-81 39.300849741857306 40.0 40.0 40.0 40.0 40.0 82-83 39.27935978712842 40.0 40.0 40.0 40.0 40.0 84-85 39.30665548586521 40.0 40.0 40.0 40.0 40.0 86-87 39.30279315869409 40.0 40.0 40.0 40.0 40.0 88-89 39.3310368421908 40.0 40.0 40.0 40.0 40.0 90-91 39.427446773598405 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16998482332802922 1101 2 0.2673041242010257 1101 3 0.16303839394848296 1101 4 0.1807055611758699 1101 5 0.3334119062416292 1101 6 -0.04344810219482298 1101 7 0.15935660491071957 1101 8 0.20430777249342214 1101 9 0.15559035793769027 1101 10-11 0.15432366541141107 1101 12-13 0.1997408034769279 1101 14-15 0.14068813285368975 1101 16-17 0.09361196658183957 1101 18-19 0.01897067510700623 1101 20-21 -0.007001455717812632 1101 22-23 0.08849475041824917 1101 24-25 0.07492839988185551 1101 26-27 0.09775339721709742 1101 28-29 0.05865490891500258 1101 30-31 0.050873652956163085 1101 32-33 0.10549546629576412 1101 34-35 0.042803148976652494 1101 36-37 0.010056190057781578 1101 38-39 0.1509308543793395 1101 40-41 0.0990462943936663 1101 42-43 0.08133327163491089 1101 44-45 -0.05758501282798534 1101 46-47 -0.02172490269411753 1101 48-49 0.05565196958327334 1101 50-51 -0.0448613641753397 1101 52-53 -0.030851450886750342 1101 54-55 -0.19297970044625856 1101 56-57 -0.37452655662951173 1101 58-59 -0.09795704185577847 1101 60-61 -0.14553038012685704 1101 62-63 0.14895646551970998 1101 64-65 0.17920133250281367 1101 66-67 0.12295173334591425 1101 68-69 0.23497035694532542 1101 70-71 0.28175135318817723 1101 72-73 0.3873889654245204 1101 74-75 0.3919518728845972 1101 76-77 0.16166207075217898 1101 78-79 0.22869092944539915 1101 80-81 0.17375264158526704 1101 82-83 -0.5275070695633488 1101 84-85 -0.39140301623564966 1101 86-87 -0.11544584797191959 1101 88-89 -0.7740238889443845 1101 90-91 0.4001432191162735 1102 1 0.3541071302483658 1102 2 0.2608495790237839 1102 3 0.09494538261032659 1102 4 0.07196660726364001 1102 5 0.18030863046843848 1102 6 0.012757686763514187 1102 7 0.054821036279321333 1102 8 -0.10238683539905935 1102 9 -0.0629002668049381 1102 10-11 -0.025531198832666746 1102 12-13 -0.061338838167699805 1102 14-15 -0.16546656270590887 1102 16-17 0.12502449315802977 1102 18-19 -0.017471882186235632 1102 20-21 0.0789117129575061 1102 22-23 0.0657086197594623 1102 24-25 0.02369468494946858 1102 26-27 0.023840313951630776 1102 28-29 0.0747333151670091 1102 30-31 0.05243735474537203 1102 32-33 -0.12452798328467907 1102 34-35 -0.02454531465001253 1102 36-37 -0.03452793594112791 1102 38-39 -0.03152847959512428 1102 40-41 0.04298692164177709 1102 42-43 0.0526480089998671 1102 44-45 -0.07163529690739523 1102 46-47 -0.04449795808121593 1102 48-49 -0.10240982307352198 1102 50-51 -0.06622877543154715 1102 52-53 -0.09591215018957655 1102 54-55 0.1055921413379437 1102 56-57 0.051425673000920824 1102 58-59 -0.04687780782612094 1102 60-61 -0.10298894312821716 1102 62-63 -0.19434344446251828 1102 64-65 -0.2484686138271499 1102 66-67 -0.4412257731324445 1102 68-69 -0.16176718709030524 1102 70-71 -0.1221551315923719 1102 72-73 -0.26113622370552037 1102 74-75 -0.6412449603122425 1102 76-77 -0.1528118937217826 1102 78-79 -0.36193666770410715 1102 80-81 0.03353123048097473 1102 82-83 -0.07795568206385894 1102 84-85 -0.3748253909623642 1102 86-87 -0.3227259288617077 1102 88-89 0.6377408169379635 1102 90-91 -0.6665234475503894 1103 1 0.3308031554228492 1103 2 0.286590636482579 1103 3 0.20098760963773543 1103 4 -0.05305392360046568 1103 5 0.08511373166614078 1103 6 -0.011380079729612191 1103 7 0.007618382209940933 1103 8 0.012409199569958673 1103 9 0.17301656498764117 1103 10-11 -0.02564873332075024 1103 12-13 0.03352455310410818 1103 14-15 -0.019100965151032767 1103 16-17 0.02854374696472206 1103 18-19 0.07050820727057072 1103 20-21 -0.09801385606763091 1103 22-23 -0.008012627612963286 1103 24-25 -0.014546215545536256 1103 26-27 0.06634962504382003 1103 28-29 -0.005846119813568862 1103 30-31 -0.025892851869208755 1103 32-33 0.08977511920829784 1103 34-35 -0.032037391356155354 1103 36-37 -0.014247544966700332 1103 38-39 0.08028577364188294 1103 40-41 -0.032858692082520236 1103 42-43 0.06468916997940966 1103 44-45 0.19925370214363625 1103 46-47 0.08954810539125901 1103 48-49 0.03993654220595744 1103 50-51 -0.03876303873326492 1103 52-53 -0.04115448120857934 1103 54-55 0.0710232005785727 1103 56-57 -0.23526994713403582 1103 58-59 -0.056886120729181755 1103 60-61 0.15776165320475144 1103 62-63 -0.016381235366957014 1103 64-65 0.04070836259383981 1103 66-67 0.04447484744451202 1103 68-69 -0.07035089199964517 1103 70-71 0.133843391162479 1103 72-73 0.3551598938436058 1103 74-75 0.08805484996798185 1103 76-77 -0.20160279325894948 1103 78-79 0.13163475106392042 1103 80-81 -0.0072154582728387595 1103 82-83 0.14693236848208358 1103 84-85 0.19853301174421745 1103 86-87 -0.4010760896147829 1103 88-89 -0.4822591830620411 1103 90-91 0.4001432191162735 1104 1 0.27110547187357525 1104 2 0.1249102281590595 1104 3 0.12338651030518832 1104 4 0.19507132178626563 1104 5 0.18437757147768252 1104 6 0.15450134940270033 1104 7 0.016962708868753396 1104 8 0.007717405302194891 1104 9 -0.11011064349292354 1104 10-11 -0.0421534447459635 1104 12-13 -0.004598729188785455 1104 14-15 0.03405961592475393 1104 16-17 -0.04139637867329071 1104 18-19 -0.040715781736103906 1104 20-21 0.03103502496888666 1104 22-23 0.10820743520604026 1104 24-25 0.001502076562829302 1104 26-27 -0.05593639772806824 1104 28-29 -0.06942091368871672 1104 30-31 0.0588931709026852 1104 32-33 0.10763044856060588 1104 34-35 0.09836878360510326 1104 36-37 -0.03837256728428429 1104 38-39 -0.04969430540653974 1104 40-41 -0.10993913238741726 1104 42-43 -0.013255601207163181 1104 44-45 -0.0310568569517784 1104 46-47 -0.05757661314950724 1104 48-49 -0.009047410276807 1104 50-51 0.110735777289797 1104 52-53 -0.1788814608450977 1104 54-55 -0.022441276556271816 1104 56-57 -0.2726515369241085 1104 58-59 -0.16527550621357534 1104 60-61 -0.05373362171140883 1104 62-63 0.0996131015912809 1104 64-65 0.08026050875975699 1104 66-67 0.15176174737355552 1104 68-69 -0.22046251075762768 1104 70-71 -0.0411044190291463 1104 72-73 -0.11693956088190305 1104 74-75 -0.010859542849047443 1104 76-77 -0.043814957427990464 1104 78-79 0.04906946688573299 1104 80-81 -0.019534465014473312 1104 82-83 -0.21845303517803671 1104 84-85 0.4324844163474424 1104 86-87 -0.03297193341405347 1104 88-89 0.1932963724935206 1104 90-91 0.4001432191162735 1201 1 0.2816291607317467 1201 2 0.18629393391042015 1201 3 0.09373512429581865 1201 4 0.27340388724736897 1201 5 0.1018677811067974 1201 6 0.03848353476705313 1201 7 0.07834641462011405 1201 8 0.04424892645707246 1201 9 0.11755003377037809 1201 10-11 0.002919695083576812 1201 12-13 0.00995244949508134 1201 14-15 -0.05521320907717353 1201 16-17 0.0707043277691568 1201 18-19 -0.02130109234041555 1201 20-21 0.0763212493411487 1201 22-23 -0.06392360218934101 1201 24-25 0.07859836773997841 1201 26-27 -0.03783668951951569 1201 28-29 -0.037291220069732844 1201 30-31 0.0785698966955124 1201 32-33 0.08369142817100084 1201 34-35 0.0038654270549685066 1201 36-37 -0.05457898424566565 1201 38-39 -0.0861259071645506 1201 40-41 0.1229668517923912 1201 42-43 0.012462559534625939 1201 44-45 -0.09054702267538062 1201 46-47 -0.09447931154897304 1201 48-49 0.018781062313884433 1201 50-51 -0.09124044666191367 1201 52-53 -0.06987988583416183 1201 54-55 0.01085764985597848 1201 56-57 0.039823914980487984 1201 58-59 -0.06366827349697957 1201 60-61 -0.039424134520125165 1201 62-63 -0.29671353595738026 1201 64-65 -0.31408239032355567 1201 66-67 -0.14024151171260968 1201 68-69 -0.19538027692683357 1201 70-71 -0.6432124497169056 1201 72-73 -0.2380884541648527 1201 74-75 -0.121124987362073 1201 76-77 -0.055243480194143046 1201 78-79 -0.36012925013404384 1201 80-81 -0.02033039568146222 1201 82-83 -0.7452015567565482 1201 84-85 0.22910674995607394 1201 86-87 0.1262586593741517 1201 88-89 0.024104453301603712 1201 90-91 0.4001432191162735 1202 1 0.16312196079271502 1202 2 -0.07115663516444215 1202 3 -0.033791464129429016 1202 4 0.011376820613882899 1202 5 0.017659849671460393 1202 6 0.059932103506021406 1202 7 -0.09469299039756152 1202 8 0.05682488582560552 1202 9 -0.002231133622267123 1202 10-11 -0.02470676900282598 1202 12-13 -0.12240373637432356 1202 14-15 -0.08848784644572305 1202 16-17 -0.056753575557031866 1202 18-19 -0.08499287877868511 1202 20-21 -0.1237499761656764 1202 22-23 0.015711595254444433 1202 24-25 -0.08240420889212885 1202 26-27 -0.12036136558223376 1202 28-29 -0.03497004007854798 1202 30-31 -0.05374959439495086 1202 32-33 -0.048652273978476046 1202 34-35 0.0031386029810676064 1202 36-37 -0.11875310093239477 1202 38-39 -0.08379717680822552 1202 40-41 -0.0855981370614316 1202 42-43 -0.23939778580468385 1202 44-45 0.10572730689095522 1202 46-47 -0.05000843220953044 1202 48-49 -0.2634508707356886 1202 50-51 -0.13395859118907083 1202 52-53 0.09925997697442313 1202 54-55 -0.20844920674431222 1202 56-57 -0.09102832776542868 1202 58-59 -0.2239218461305441 1202 60-61 -0.1505545361086007 1202 62-63 -0.17916108742107184 1202 64-65 -0.05807036654503861 1202 66-67 -0.45241082388191955 1202 68-69 -0.287621037742511 1202 70-71 -0.34733986033904785 1202 72-73 -0.1527943289317193 1202 74-75 -0.34167840580592923 1202 76-77 -0.24885813051957228 1202 78-79 -0.19509904290840296 1202 80-81 -0.1356421537549437 1202 82-83 -0.3110247783219293 1202 84-85 -0.0994825742886789 1202 86-87 -0.1501237471175827 1202 88-89 0.002820182017330808 1202 90-91 -0.9476828678402498 1203 1 0.17576497008145964 1203 2 0.18745503102644534 1203 3 0.08234708378189026 1203 4 0.276164511750423 1203 5 0.06851735642391787 1203 6 0.18425045334534929 1203 7 0.19778708162861136 1203 8 -0.1499886878877703 1203 9 0.17564204467911537 1203 10-11 -6.211866814425093E-4 1203 12-13 0.0832148908828998 1203 14-15 0.07375495642653362 1203 16-17 -0.11742684462310393 1203 18-19 -0.004380656288070384 1203 20-21 -0.03872303954724998 1203 22-23 0.054488797213210205 1203 24-25 0.011179495917666316 1203 26-27 -0.05544356618684532 1203 28-29 -0.04603381691452313 1203 30-31 -0.0186157896707968 1203 32-33 0.021117153540437528 1203 34-35 0.033483850616569555 1203 36-37 -0.029583933856685007 1203 38-39 -0.023605552868794177 1203 40-41 -0.02984233503152467 1203 42-43 -0.026904846683393657 1203 44-45 -0.06322732889837823 1203 46-47 -0.023336992110095878 1203 48-49 -0.045737914656555745 1203 50-51 -0.0372907235598916 1203 52-53 0.11889410123001909 1203 54-55 0.06041494467454811 1203 56-57 -0.06976002147033 1203 58-59 0.007423886237475585 1203 60-61 -0.09137894151565007 1203 62-63 0.1981696326645377 1203 64-65 0.020774748567816914 1203 66-67 0.21534656253817985 1203 68-69 0.332553424898137 1203 70-71 0.006411631869205792 1203 72-73 0.04819583915915615 1203 74-75 -0.1459094994595347 1203 76-77 -0.19085866966548792 1203 78-79 -0.17155154303231512 1203 80-81 -0.03043084527180895 1203 82-83 -0.21224200834485885 1203 84-85 -0.6686858818671979 1203 86-87 0.3500913364874876 1203 88-89 -0.2731077330083309 1203 90-91 0.4001432191162735 1204 1 0.3671050211185616 1204 2 0.0862805234035946 1204 3 0.03980526171379495 1204 4 0.11514682325121584 1204 5 -0.24451019052366973 1204 6 -0.08149143851706242 1204 7 -0.0784621277325499 1204 8 -0.0686292253040719 1204 9 -0.11131077871942807 1204 10-11 -0.11984952317732933 1204 12-13 -0.09373553333573881 1204 14-15 -0.25203459187331134 1204 16-17 -0.10126509627527014 1204 18-19 0.04772516935697979 1204 20-21 -0.07872663831712146 1204 22-23 -0.07549495379554116 1204 24-25 -0.12708142580363102 1204 26-27 -0.06584455639383435 1204 28-29 0.011055759739278415 1204 30-31 -0.16361077320369333 1204 32-33 -0.08124321436343962 1204 34-35 -0.13177884217370917 1204 36-37 -0.12472685176956588 1204 38-39 -0.12910133012241687 1204 40-41 8.998492383156531E-4 1204 42-43 -0.05980092262417003 1204 44-45 -0.273595685283091 1204 46-47 -0.018382864893744966 1204 48-49 -0.053291472108192295 1204 50-51 0.027471407682121196 1204 52-53 0.07602172001182339 1204 54-55 -0.06309203253730544 1204 56-57 -0.119264529640013 1204 58-59 -0.12197946084845768 1204 60-61 -0.23266082628307316 1204 62-63 -0.2068256768984469 1204 64-65 -0.37138491876276447 1204 66-67 0.042301835952500255 1204 68-69 -0.20803312914609506 1204 70-71 -0.13177089198003245 1204 72-73 -0.22106760592370733 1204 74-75 -0.040303220454923405 1204 76-77 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0.17988228291368813 2401 54-55 0.33188577642172135 2401 56-57 -0.04152928908003162 2401 58-59 0.11709235502026871 2401 60-61 0.1698984550911362 2401 62-63 0.17101849012410497 2401 64-65 0.34060853409267366 2401 66-67 0.20366991427991366 2401 68-69 0.1531046472217099 2401 70-71 0.31417963629668577 2401 72-73 0.23589566940108853 2401 74-75 0.4289834427829007 2401 76-77 0.28062149860892305 2401 78-79 0.22049217362178553 2401 80-81 0.3160570617566023 2401 82-83 0.3699152144334832 2401 84-85 0.12390470182713642 2401 86-87 -0.19500881439790874 2401 88-89 0.47107415027129207 2401 90-91 0.06680988578294489 2402 1 0.40192540523587894 2402 2 0.3479147189601619 2402 3 0.267070415853496 2402 4 0.2827324835999647 2402 5 0.263832156440742 2402 6 0.15755799144239546 2402 7 0.11654260166898922 2402 8 0.25539957247588774 2402 9 0.21986545385605183 2402 10-11 0.14913838489332676 2402 12-13 0.22044835179050182 2402 14-15 0.1571075660913479 2402 16-17 0.12192210604350606 2402 18-19 0.1520029637368836 2402 20-21 0.10060803670290852 2402 22-23 0.0638553419132677 2402 24-25 0.08393362806217652 2402 26-27 0.017685578224416076 2402 28-29 0.1350445361157142 2402 30-31 0.04589157739261651 2402 32-33 0.09941482082290065 2402 34-35 0.15503976716971124 2402 36-37 0.08503493498371029 2402 38-39 0.16901967862985856 2402 40-41 0.1781752313534426 2402 42-43 0.0580955315544287 2402 44-45 0.16041588744310786 2402 46-47 -0.08541237727290252 2402 48-49 0.14255366119694202 2402 50-51 0.18509051684531386 2402 52-53 0.15543331387053172 2402 54-55 0.07895410570679928 2402 56-57 0.08594095900735965 2402 58-59 0.0764531488759772 2402 60-61 0.2288499424488606 2402 62-63 0.22995947851424603 2402 64-65 0.22928141711213357 2402 66-67 0.26387585240475175 2402 68-69 0.19198083155869483 2402 70-71 0.17182378677457422 2402 72-73 0.06051066500609181 2402 74-75 0.18094577376410825 2402 76-77 0.19674401403461417 2402 78-79 0.2155327371358169 2402 80-81 0.34392004004482857 2402 82-83 0.280840486033064 2402 84-85 0.43688472146717316 2402 86-87 0.21515350436656178 2402 88-89 0.016066579429846684 2402 90-91 0.4001432191162735 2403 1 -0.3221908400435751 2403 2 -0.13432403773906287 2403 3 0.11392261790160774 2403 4 -0.08026374546150095 2403 5 0.03292600392531142 2403 6 0.1124328363514806 2403 7 0.08332945520175628 2403 8 0.1623052491561694 2403 9 0.10310108151762165 2403 10-11 0.16382328799690526 2403 12-13 0.07485672795541376 2403 14-15 0.18393543016158986 2403 16-17 0.1432906966348355 2403 18-19 0.14417514567380607 2403 20-21 0.06367069205731468 2403 22-23 0.08509892496657301 2403 24-25 0.11843937446807473 2403 26-27 0.08632718069641498 2403 28-29 0.09059244213864304 2403 30-31 0.07863402098937655 2403 32-33 0.08466331733599475 2403 34-35 0.1475393632810622 2403 36-37 0.07838347779426158 2403 38-39 0.11484720523571923 2403 40-41 0.054146026742301956 2403 42-43 0.015744379879663484 2403 44-45 0.14186246440025485 2403 46-47 0.123562048086157 2403 48-49 0.14997050587658123 2403 50-51 0.10571160826115289 2403 52-53 0.26360690551606325 2403 54-55 0.09006877178990891 2403 56-57 -0.008211430214672077 2403 58-59 0.02932396765437062 2403 60-61 0.08542885161345737 2403 62-63 0.21579987629868214 2403 64-65 0.16370925979354922 2403 66-67 0.17031125022215576 2403 68-69 0.21358258538414532 2403 70-71 0.16238650442087277 2403 72-73 0.2670319838573647 2403 74-75 0.2797468183870464 2403 76-77 0.061876997153056834 2403 78-79 0.05121609711584796 2403 80-81 0.2619662462257253 2403 82-83 0.07963672552855172 2403 84-85 0.23666634048040436 2403 86-87 -0.04244875644494783 2403 88-89 0.32345510265224675 2403 90-91 -0.2152413962683397 2404 1 -0.33208121735372487 2404 2 -0.2691112044410531 2404 3 -0.42294738704394064 2404 4 -0.24577752198135983 2404 5 -0.0980674369825536 2404 6 -0.15508691906881467 2404 7 -0.06233791154861734 2404 8 0.11502140981431808 2404 9 -0.006853452291572637 2404 10-11 0.0818524774198579 2404 12-13 0.050110802678105415 2404 14-15 0.09350688044815314 2404 16-17 -7.517167949089298E-5 2404 18-19 0.04146836942013721 2404 20-21 -0.0350182745235017 2404 22-23 0.053335748798822635 2404 24-25 0.033524073178732294 2404 26-27 -0.031775371219325166 2404 28-29 0.04348094756339549 2404 30-31 0.03957563001153375 2404 32-33 -0.05302971345862062 2404 34-35 0.09572101506055475 2404 36-37 0.1608649485564797 2404 38-39 0.024452177134413944 2404 40-41 0.03161177649562319 2404 42-43 0.1444572568893605 2404 44-45 -0.03278977758581192 2404 46-47 0.09939592870078684 2404 48-49 0.027081194122779095 2404 50-51 -0.04057109944063342 2404 52-53 0.12716458810542264 2404 54-55 0.08436108577784296 2404 56-57 0.23011790683848687 2404 58-59 0.17163056100064722 2404 60-61 0.04828729778429164 2404 62-63 0.1882990042251862 2404 64-65 0.14965341007339106 2404 66-67 0.057637075653452996 2404 68-69 0.1991487782792305 2404 70-71 0.3561215422017838 2404 72-73 0.4060572621779386 2404 74-75 0.013671171714314312 2404 76-77 0.2957619853632778 2404 78-79 0.3630864942504317 2404 80-81 0.29057099147215126 2404 82-83 0.4695238168544833 2404 84-85 0.14417412803715024 2404 86-87 0.2730616526637277 2404 88-89 0.19785811899075156 2404 90-91 -0.09985678088372651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 9.0 31 377.0 32 2765.0 33 4710.0 34 6313.0 35 7931.0 36 11837.0 37 19582.0 38 71269.0 39 312929.0 40 140542.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.746631249827068 12.841544229545393 30.883207437536313 29.528617083091223 2 27.436066390299835 17.323945854238115 28.954181561593657 26.285806193868392 3 27.63040027115924 18.790055649900218 22.097921688934697 31.481622390005846 4 26.945132327103195 20.0066059792759 20.665474592919498 32.38278710070141 5 27.323506218612952 20.339325982596186 20.57485854211917 31.762309256671696 6 27.941563023117467 22.52085552619565 23.402978570341574 26.13460288034531 7 26.3531881631919 22.323022010707913 23.23143062684172 28.09235919925847 8 25.61719214753123 23.30198663586182 23.596834663752198 27.48398655285475 9 25.460170441182573 23.441369339955454 24.151598577812212 26.946861641049765 10-11 25.346468049195526 22.41951772892658 23.964486808793215 28.26952741308468 12-13 25.660597927590167 22.20750383907696 23.822423668082397 28.309474565250476 14-15 25.98164506177109 21.750619094392874 23.76302173401768 28.50471410981835 16-17 26.02340799358079 21.64236404133752 23.57980092137847 28.754427043703224 18-19 26.10978722521201 21.82134803480763 23.634620173484773 28.434244566495583 20-21 26.16469294301565 21.91559564489576 23.617932293900363 28.30177911818823 22-23 25.830935351327422 21.48914682567132 24.026569179475118 28.65334864352614 24-25 25.94420541482783 21.676172128992985 24.108452194845263 28.27117026133392 26-27 26.03240042610296 21.86466734916924 23.978407786063112 28.124524438664693 28-29 25.972306766459607 21.53471424816347 24.15868876499315 28.334290220383767 30-31 26.21967089227087 21.8355741054421 24.06553793662199 27.879217065665042 32-33 26.283314682490342 21.830657400328217 24.132868076370574 27.753159840810866 34-35 26.203201266475034 21.5353465221465 24.235966400606337 28.02548581077213 36-37 26.292113467273108 21.800449561733785 24.212890448507483 27.69454652248562 38-39 26.41465808959681 21.895433160968977 24.147011970146153 27.54289677928806 40-41 26.377715009252945 21.665222762760607 24.21278188165456 27.744280346331884 42-43 26.353460419223808 22.016152865472602 24.219590958019378 27.410795757284212 44-45 26.647126915247277 21.899423501302966 24.16116873563743 27.29228084781233 46-47 26.484349721822653 21.825924359513472 24.0492830162156 27.640442902448275 48-49 26.69120828162928 21.969840237713434 24.14218765786467 27.19676382279261 50-51 26.900099545432397 21.948862884868397 23.949517239458974 27.201520330240232 52-53 26.58580784876945 21.82270714119213 24.14289903676244 27.448585973275986 54-55 26.923743245937757 21.908004197079695 23.99575715775332 27.17249539922923 56-57 27.01962321991908 22.031653804338138 23.819487764450066 27.129235211292706 58-59 26.790970389914982 21.88136421381804 23.946643213133978 27.381022183133002 60-61 27.075482471165923 22.141208865791466 23.71385910139813 27.069449561644486 62-63 27.248517244429358 22.068375291949252 23.625597345502968 27.057510118118422 64-65 27.130163163702075 21.850396848664467 23.588641972676346 27.43079801495712 66-67 27.244736124144424 22.0841440636552 23.54960470338513 27.12151510881525 68-69 27.465996772575025 22.0441262221499 23.342554547550275 27.147322457724798 70-71 27.405383227924045 21.88745848783471 23.374542301767313 27.33261598247393 72-73 27.600884343107264 21.997811474128497 23.21043346211394 27.190870720650306 74-75 27.84051013455527 21.75301540077914 23.09492726109548 27.311547203570107 76-77 27.860855854746124 21.853366509817448 22.96383434751546 27.32194328792097 78-79 28.304429601765325 21.943634021000253 22.651540956414852 27.100395420819574 80-81 28.52749787210675 21.83996412326222 22.351573725780913 27.28096427885011 82-83 28.790802566725564 21.234771691620942 22.296103413001024 27.67832232865247 84-85 29.756906248547484 21.018545775684427 21.48335218381544 27.74119579195265 86-87 30.44065843986087 20.09216385670293 20.894166648204358 28.573011055231852 88-89 30.562043795620436 19.01824817518248 20.587591240875913 29.832116788321166 90-91 31.160714285714285 19.294642857142858 21.821428571428573 27.72321428571429 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 3.5 12 6.0 13 10.5 14 16.5 15 26.0 16 40.5 17 60.5 18 89.5 19 131.5 20 188.5 21 257.5 22 332.5 23 440.0 24 592.5 25 752.5 26 985.0 27 1291.0 28 1603.5 29 1964.5 30 2402.5 31 2897.5 32 3411.0 33 3927.5 34 4727.0 35 5787.0 36 6922.0 37 8083.0 38 9404.5 39 11123.0 40 13043.5 41 14990.0 42 17199.0 43 19577.5 44 22029.0 45 24879.5 46 27835.5 47 30705.5 48 33734.5 49 36704.0 50 39239.5 51 41579.0 52 43427.0 53 44701.5 54 45570.0 55 45893.5 56 45629.5 57 44753.0 58 43288.0 59 41363.0 60 39540.0 61 37513.0 62 35306.0 63 33098.0 64 30526.0 65 27736.0 66 24731.5 67 22059.0 68 19641.0 69 16986.5 70 14354.5 71 11787.0 72 9488.5 73 7522.5 74 5837.5 75 4537.0 76 3464.0 77 2536.5 78 1895.5 79 1399.0 80 959.0 81 644.5 82 433.5 83 292.0 84 205.5 85 149.5 86 98.0 87 61.0 88 41.5 89 22.5 90 9.5 91 7.0 92 5.0 93 2.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001383451157256893 2 0.008127775548884247 3 0.0010375883679426697 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.4663593811412444E-4 74-75 5.201181708484168E-4 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7300.0 32-33 16317.0 34-35 18447.0 36-37 20289.0 38-39 22402.0 40-41 23847.0 42-43 24834.0 44-45 25606.0 46-47 26521.0 48-49 26937.0 50-51 26583.0 52-53 26790.0 54-55 25634.0 56-57 24826.0 58-59 24072.0 60-61 23050.0 62-63 21954.0 64-65 21114.0 66-67 19576.0 68-69 18512.0 70-71 17635.0 72-73 16066.0 74-75 15171.0 76-77 13936.0 78-79 13184.0 80-81 11841.0 82-83 11008.0 84-85 9979.0 86-87 9012.0 88-89 8298.0 90-91 7523.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.75309997621534 #Duplication Level Percentage of deduplicated Percentage of total 1 99.76700855832755 99.52068379046779 2 0.22267475441382803 0.44424994078443564 3 0.007981530598762948 0.02388547259344866 4 9.771386601569731E-4 0.0038989044182905458 5 8.481927698027732E-4 0.0042304929082619515 6 5.098252299095097E-4 0.003051398827773617 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001383451157256893 0.0 0.0 0.0 0.0 2 0.001383451157256893 0.0 0.0 0.0 0.0 3 0.001383451157256893 0.0 0.0 0.0 0.0 4 0.001383451157256893 0.0 0.0 0.0 0.0 5 0.001383451157256893 0.0 0.0 0.0 0.0 6 0.0015563825519140048 0.0 0.0 0.0 0.0 7 0.0017293139465711163 0.0 0.0 0.0 1.7293139465711163E-4 8 0.003977422077113568 0.0 0.0 0.0 1.7293139465711163E-4 9 0.004150353471770679 0.0 0.0 0.0 3.4586278931422325E-4 10-11 0.004150353471770679 0.0 0.0 0.0 3.4586278931422325E-4 12-13 0.004150353471770679 0.0 0.0 0.0 6.917255786284465E-4 14-15 0.004150353471770679 0.0 0.0 0.0 8.646569732855582E-4 16-17 0.004323284866427791 0.0 0.0 0.0 8.646569732855582E-4 18-19 0.004323284866427791 0.0 0.0 0.0 8.646569732855582E-4 20-21 0.004669147655742014 0.0 0.0 0.0 8.646569732855582E-4 22-23 0.004669147655742014 0.0 8.646569732855581E-5 0.0 8.646569732855582E-4 24-25 0.00475561335307057 0.0 1.7293139465711163E-4 0.0 8.646569732855582E-4 26-27 0.005015010445056237 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 28-29 0.005187941839713349 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 30-31 0.005533804629027572 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 32-33 0.005879667418341795 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 34-35 0.006484927299641686 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 36-37 0.006917255786284465 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 38-39 0.007176652878270132 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 40-41 0.007608981364912911 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 42-43 0.008300706943541358 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 44-45 0.00847363833819847 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 46-47 0.008905966824841249 0.0 1.7293139465711163E-4 0.0 0.0010375883679426697 48-49 0.008992432522169806 0.0 1.7293139465711163E-4 0.0 0.0012105197625997815 50-51 0.009165363916826916 0.0 1.7293139465711163E-4 0.0 0.0012105197625997815 52-53 0.009424761008812583 0.0 1.7293139465711163E-4 0.0 0.0012105197625997815 54-55 0.010030020890112474 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 56-57 0.010202952284769586 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 58-59 0.010635280771412365 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 60-61 0.0111540749553837 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 62-63 0.011932266231340702 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 64-65 0.012105197625997814 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 66-67 0.012451060415312038 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 68-69 0.01279692320462626 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 70-71 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 72-73 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 74-75 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 76-77 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 78-79 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 80 0.012969854599283372 0.0 3.4586278931422325E-4 0.0 0.0012105197625997815 >>END_MODULE