##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-16B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 663029 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25112174580599 40.0 40.0 40.0 40.0 40.0 2 39.147560664767305 40.0 40.0 40.0 40.0 40.0 3 39.14093048720343 40.0 40.0 40.0 40.0 40.0 4 39.064776955457454 40.0 40.0 40.0 40.0 40.0 5 39.214212349686065 40.0 40.0 40.0 40.0 40.0 6 39.264249376724095 40.0 40.0 40.0 40.0 40.0 7 39.30123720078609 40.0 40.0 40.0 40.0 40.0 8 39.31637982652342 40.0 40.0 40.0 40.0 40.0 9 39.33878005336116 40.0 40.0 40.0 40.0 40.0 10-11 39.34720502421463 40.0 40.0 40.0 40.0 40.0 12-13 39.34162759095002 40.0 40.0 40.0 40.0 40.0 14-15 39.35300265900888 40.0 40.0 40.0 40.0 40.0 16-17 39.480345505249396 40.0 40.0 40.0 40.0 40.0 18-19 39.496758060356335 40.0 40.0 40.0 40.0 40.0 20-21 39.49967799296863 40.0 40.0 40.0 40.0 40.0 22-23 39.50319820098367 40.0 40.0 40.0 40.0 40.0 24-25 39.50039289382516 40.0 40.0 40.0 40.0 40.0 26-27 39.489078154952495 40.0 40.0 40.0 40.0 40.0 28-29 39.48089751730316 40.0 40.0 40.0 40.0 40.0 30-31 39.47015149779388 40.0 40.0 40.0 40.0 40.0 32-33 39.46640893608986 40.0 40.0 40.0 40.0 40.0 34-35 39.469581466627034 40.0 40.0 40.0 40.0 40.0 36-37 39.45945588139206 40.0 40.0 40.0 40.0 40.0 38-39 39.44193075954395 40.0 40.0 40.0 40.0 40.0 40-41 39.43301782908465 40.0 40.0 40.0 40.0 40.0 42-43 39.437748653566466 40.0 40.0 40.0 40.0 40.0 44-45 39.415455719775025 40.0 40.0 40.0 40.0 40.0 46-47 39.409648772144614 40.0 40.0 40.0 40.0 40.0 48-49 39.4017742632069 40.0 40.0 40.0 40.0 40.0 50-51 39.405950880260455 40.0 40.0 40.0 40.0 40.0 52-53 39.40374194973772 40.0 40.0 40.0 40.0 40.0 54-55 39.372805652438444 40.0 40.0 40.0 40.0 40.0 56-57 39.38150723428342 40.0 40.0 40.0 40.0 40.0 58-59 39.35643741631185 40.0 40.0 40.0 40.0 40.0 60-61 39.32005237842739 40.0 40.0 40.0 40.0 40.0 62-63 39.35738627292981 40.0 40.0 40.0 40.0 40.0 64-65 39.337236143662 40.0 40.0 40.0 40.0 40.0 66-67 39.33586075166495 40.0 40.0 40.0 40.0 40.0 68-69 39.347648682585884 40.0 40.0 40.0 40.0 40.0 70-71 39.336315095280185 40.0 40.0 40.0 40.0 40.0 72-73 39.327880144910026 40.0 40.0 40.0 40.0 40.0 74-75 39.33052363118975 40.0 40.0 40.0 40.0 40.0 76-77 39.316048480761154 40.0 40.0 40.0 40.0 40.0 78-79 39.32921753794881 40.0 40.0 40.0 40.0 40.0 80-81 39.30100904944865 40.0 40.0 40.0 40.0 40.0 82-83 39.3324659671171 40.0 40.0 40.0 40.0 40.0 84-85 39.30144024273857 40.0 40.0 40.0 40.0 40.0 86-87 39.31089268954652 40.0 40.0 40.0 40.0 40.0 88-89 39.33402047937313 40.0 40.0 40.0 40.0 40.0 90-91 39.39740072593315 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19259853556953743 1101 2 0.19060000049605463 1101 3 0.035963116204698053 1101 4 0.022704520376869652 1101 5 0.018628555463827468 1101 6 -0.11475718870926244 1101 7 -0.017618311626627303 1101 8 -0.06521296838512569 1101 9 -0.02894149389120315 1101 10-11 -0.056360850119098416 1101 12-13 -0.08846943293346499 1101 14-15 -0.10309894874055914 1101 16-17 -0.09387677268193784 1101 18-19 -0.06401609179054901 1101 20-21 -0.0209976447455702 1101 22-23 0.08751472294316187 1101 24-25 -0.03434804028016458 1101 26-27 -0.08020412396984256 1101 28-29 -0.012617810297641086 1101 30-31 -0.07409658192004542 1101 32-33 -3.766626936965167E-4 1101 34-35 -0.011532849281955748 1101 36-37 0.07922261648354834 1101 38-39 0.017508690329293586 1101 40-41 -0.009713796429153376 1101 42-43 -0.014173965072245664 1101 44-45 0.13071019616754853 1101 46-47 -0.04493502131729343 1101 48-49 0.08122297182283944 1101 50-51 -0.10025291518441293 1101 52-53 -0.10107458924889556 1101 54-55 0.14378716302655192 1101 56-57 0.09170396412154247 1101 58-59 0.015453296766786195 1101 60-61 -0.1247001227238016 1101 62-63 0.18805025691516875 1101 64-65 -0.05222519832925343 1101 66-67 -0.019009835769146832 1101 68-69 0.12700072206337865 1101 70-71 -0.07505334230884841 1101 72-73 -0.12250690177731371 1101 74-75 -0.008306744180053727 1101 76-77 -0.025964539958678756 1101 78-79 0.0523040448600085 1101 80-81 0.24668840652821444 1101 82-83 0.1701364803642278 1101 84-85 0.4627530242795359 1101 86-87 0.10890358056555272 1101 88-89 -0.5371222579975878 1101 90-91 -1.2072215215984414 1102 1 0.34803987488647437 1102 2 -0.062270380667250436 1102 3 -0.02035172235457594 1102 4 0.05874456000853456 1102 5 0.1413555632735921 1102 6 0.001128986835674084 1102 7 0.13387078966723465 1102 8 -0.03385733253013257 1102 9 0.031249087761672456 1102 10-11 -0.11136070441447288 1102 12-13 -0.004525354369533829 1102 14-15 -0.014066136058431766 1102 16-17 0.005471927632790141 1102 18-19 -0.028161825559053 1102 20-21 0.008930085759153883 1102 22-23 0.0072970402295595704 1102 24-25 -0.05941652145512677 1102 26-27 0.012077901907296962 1102 28-29 0.012002629147509936 1102 30-31 0.011847292657407138 1102 32-33 -0.06898608859450661 1102 34-35 -0.028878999641257508 1102 36-37 0.13017677139893635 1102 38-39 -0.012118509900361119 1102 40-41 -0.018257222968074416 1102 42-43 -0.005385757186104456 1102 44-45 0.030973667153702422 1102 46-47 -0.16138868632133097 1102 48-49 -0.08627001191634065 1102 50-51 -0.015289809379005703 1102 52-53 -0.14624491113901428 1102 54-55 -0.14481519649170593 1102 56-57 -0.21597955735038 1102 58-59 -0.2509031120509988 1102 60-61 -0.0973488490583918 1102 62-63 -0.3423724085781643 1102 64-65 0.047616587305839175 1102 66-67 0.22585818302047755 1102 68-69 0.028262224065571218 1102 70-71 0.1540407627984024 1102 72-73 -0.04796444840074088 1102 74-75 0.07708205248693645 1102 76-77 -0.3720570439459152 1102 78-79 -0.3700346261422993 1102 80-81 -0.2905671260238947 1102 82-83 0.047874813142080086 1102 84-85 -0.9020443911082197 1102 86-87 -0.38528150416717466 1102 88-89 0.3423243823186155 1102 90-91 0.57055625617933 1103 1 0.3400075971278653 1103 2 0.22580949970684117 1103 3 0.07448012007899507 1103 4 -0.05768542079115946 1103 5 0.01366729846856174 1103 6 0.05180365215743166 1103 7 0.17125742377299957 1103 8 0.048174892140060876 1103 9 -0.01199142931952224 1103 10-11 0.017547709215101293 1103 12-13 0.07969942199549962 1103 14-15 0.04072907536619397 1103 16-17 0.002684785647815602 1103 18-19 -0.018660768214715517 1103 20-21 -0.11281139133762252 1103 22-23 0.04915017996712123 1103 24-25 -0.06815310556929433 1103 26-27 0.018226425606854946 1103 28-29 -0.03962939732591053 1103 30-31 -0.05146241632957782 1103 32-33 -0.07903542165838218 1103 34-35 -0.051398133887239794 1103 36-37 -0.08629284742970356 1103 38-39 -0.07387230406675371 1103 40-41 -0.05084978496978465 1103 42-43 -0.027719612817037387 1103 44-45 0.0475185111304981 1103 46-47 -0.04501641288030811 1103 48-49 0.21300557903464323 1103 50-51 -0.07382992342071049 1103 52-53 0.04101004702411615 1103 54-55 0.04373055294382766 1103 56-57 0.07901672982374919 1103 58-59 0.16951421270977818 1103 60-61 0.04472816149295511 1103 62-63 -0.06149693785250321 1103 64-65 -0.23574949443144533 1103 66-67 -0.24644834384394443 1103 68-69 -1.0034453935669063 1103 70-71 -0.714141709490832 1103 72-73 -0.5598964668079134 1103 74-75 -0.28634776901910897 1103 76-77 -0.17453534009253246 1103 78-79 -0.15832214109825316 1103 80-81 -0.10157749947936878 1103 82-83 0.29368472462362405 1103 84-85 0.35068405876229747 1103 86-87 0.5044739017172688 1103 88-89 -0.5730074254617037 1103 90-91 -0.044828359205283164 1104 1 0.24102244861301614 1104 2 0.3239695657134476 1104 3 0.28987615968295444 1104 4 0.251291476898615 1104 5 0.22656333807252338 1104 6 0.25035150694291275 1104 7 0.19846864489511518 1104 8 0.23641746639748362 1104 9 0.16127589741314807 1104 10-11 -0.013099980553882062 1104 12-13 0.06500882793609719 1104 14-15 0.0012488773463914526 1104 16-17 0.032536270796327926 1104 18-19 0.14196216907901515 1104 20-21 0.17911105090660584 1104 22-23 0.05202559250837879 1104 24-25 -0.058206735932337494 1104 26-27 0.015285006464942796 1104 28-29 3.1697231105454193E-4 1104 30-31 0.018566461558215508 1104 32-33 0.033473567316555375 1104 34-35 0.026077680553548532 1104 36-37 0.09987308610948276 1104 38-39 0.06197257618740082 1104 40-41 0.021316880671335525 1104 42-43 -0.0018927322364561405 1104 44-45 0.09616565104166597 1104 46-47 -0.04586039471313086 1104 48-49 0.15361125070097614 1104 50-51 0.10495472001544925 1104 52-53 0.04476926155810901 1104 54-55 0.029848165635065982 1104 56-57 0.16126599045594503 1104 58-59 0.13266937193565553 1104 60-61 -0.03168733623335385 1104 62-63 -0.06559444676928905 1104 64-65 -0.12597282345935668 1104 66-67 -0.18720476907535044 1104 68-69 -0.12131714514859482 1104 70-71 -0.11759785546341561 1104 72-73 0.16841713342959963 1104 74-75 0.2213671478486603 1104 76-77 0.08028443373969196 1104 78-79 -0.05753465413310721 1104 80-81 0.35357894108729226 1104 82-83 0.5240652893325475 1104 84-85 0.5873340379028917 1104 86-87 -0.4853454743090069 1104 88-89 0.6901504692751388 1104 90-91 -0.3627770771540071 1201 1 0.3547708802315839 1201 2 0.16649465981632972 1201 3 0.25771179330905625 1201 4 0.0882049022436533 1201 5 0.17180486881279933 1201 6 0.2661106035552052 1201 7 0.15649248429285478 1201 8 0.08671545886925003 1201 9 -0.05641544512135965 1201 10-11 0.040900960474978376 1201 12-13 0.06397212781062933 1201 14-15 -0.026244849127884606 1201 16-17 -0.02826046697029483 1201 18-19 -0.0012075297917562011 1201 20-21 -0.04422332801434692 1201 22-23 -0.00989881150668026 1201 24-25 -0.030178191390305642 1201 26-27 -0.06434642389892531 1201 28-29 -0.040550342619937396 1201 30-31 0.10569789192207679 1201 32-33 0.008451295828805883 1201 34-35 -0.07184773662726229 1201 36-37 0.04511416002871016 1201 38-39 0.03772521961944619 1201 40-41 -0.1701047268196163 1201 42-43 0.03635864301477909 1201 44-45 -0.1768261149274295 1201 46-47 -0.00850769707064103 1201 48-49 -0.05798483743220828 1201 50-51 -0.12759250710720238 1201 52-53 0.04417384525878987 1201 54-55 -0.10127945306314956 1201 56-57 -0.055913916462365876 1201 58-59 0.14039043133097806 1201 60-61 0.14042552515594053 1201 62-63 0.09969272871350654 1201 64-65 -0.05005667355891319 1201 66-67 -0.23581526959912225 1201 68-69 -0.2059821242014266 1201 70-71 -0.19186369545855086 1201 72-73 -0.09670717142431329 1201 74-75 -0.02301048159268504 1201 76-77 -0.3927906962585155 1201 78-79 -0.0693841079047317 1201 80-81 -0.37682006395564116 1201 82-83 -0.11980397195593895 1201 84-85 0.16432042239866007 1201 86-87 -0.715902620984842 1201 88-89 -1.0241352450105765 1201 90-91 -1.42944374382067 1202 1 0.18331092194225818 1202 2 0.2648509268415893 1202 3 0.11384611676449907 1202 4 0.17309405200590788 1202 5 0.12170282305106639 1202 6 0.15892293551434022 1202 7 0.06789537696133152 1202 8 0.14908607461330092 1202 9 0.08739484284539856 1202 10-11 0.00423064482932034 1202 12-13 0.05328059372395444 1202 14-15 0.11269124399262154 1202 16-17 -0.07719089045443894 1202 18-19 -0.025155396831465282 1202 20-21 -0.004636649890457534 1202 22-23 -0.004388417301548486 1202 24-25 -0.012120745742265626 1202 26-27 0.028253277464322935 1202 28-29 0.0016880636660516757 1202 30-31 -0.031659626731176616 1202 32-33 -0.00698299787904233 1202 34-35 0.03313786303530719 1202 36-37 0.05456663662953076 1202 38-39 0.2215797077273436 1202 40-41 0.04943767689266565 1202 42-43 0.0019047887326948398 1202 44-45 0.0639583854648933 1202 46-47 0.0741367315583048 1202 48-49 0.03610839540959887 1202 50-51 0.10400264776172463 1202 52-53 0.048859834314527006 1202 54-55 0.0031597365560358526 1202 56-57 0.09115138112993293 1202 58-59 0.10905961052299773 1202 60-61 0.04474443181484844 1202 62-63 0.13876498660297187 1202 64-65 0.007528051565131477 1202 66-67 0.24935585776736957 1202 68-69 0.043727806040166683 1202 70-71 -0.03986805915127434 1202 72-73 0.09422299177276727 1202 74-75 -0.025951443884189018 1202 76-77 0.021558826755949667 1202 78-79 0.027377710689975743 1202 80-81 0.32664615165450783 1202 82-83 0.11841380817623559 1202 84-85 0.39575818597415946 1202 86-87 -0.19393879669542713 1202 88-89 0.4142884003096228 1202 90-91 -0.044828359205283164 1203 1 0.209406969497536 1203 2 0.21221895146283032 1203 3 0.20096952577632266 1203 4 0.2528148184219532 1203 5 0.13032869433787653 1203 6 0.2266660032574066 1203 7 0.10115906258553053 1203 8 -0.01158621910619928 1203 9 0.1649614010986511 1203 10-11 0.07308245187855533 1203 12-13 -0.03385787968061038 1203 14-15 0.02572676432428267 1203 16-17 0.040877794137848866 1203 18-19 0.07336573173257932 1203 20-21 0.05706559636114861 1203 22-23 0.07575716498995178 1203 24-25 0.10365137217577569 1203 26-27 0.07897358140350974 1203 28-29 0.0363519845960667 1203 30-31 -0.004452580210831059 1203 32-33 0.09258709615860283 1203 34-35 0.03580788119304401 1203 36-37 0.009252122183269762 1203 38-39 0.01657094159749306 1203 40-41 0.05978786132931191 1203 42-43 -0.01376202465149845 1203 44-45 -0.026690150385142886 1203 46-47 0.11622417147852104 1203 48-49 0.02783602952362685 1203 50-51 0.0988124842478939 1203 52-53 0.09128112307418235 1203 54-55 0.08355000500035459 1203 56-57 0.20246304680999572 1203 58-59 0.02385593851068535 1203 60-61 0.12375682589311054 1203 62-63 0.03083598136439747 1203 64-65 -0.00228096880276496 1203 66-67 -0.007412546450474622 1203 68-69 0.0681492282183811 1203 70-71 -0.12980040166745255 1203 72-73 -0.10064206988634794 1203 74-75 -0.16731929765505527 1203 76-77 -0.1449586524158306 1203 78-79 0.037192780412873105 1203 80-81 -0.15331159347177703 1203 82-83 0.1743324719380368 1203 84-85 0.27093966100319733 1203 86-87 0.11078063845878461 1203 88-89 0.2901504692751402 1203 90-91 0.57055625617933 1204 1 0.2091935091282906 1204 2 0.054749973143046304 1204 3 -0.09741862761482878 1204 4 -0.3661201468281732 1204 5 -0.04798579314377349 1204 6 -0.18756341217033423 1204 7 -0.023541540844846054 1204 8 -0.0978021261729154 1204 9 -0.10325376106498396 1204 10-11 -0.08193835682710215 1204 12-13 -0.12432385630596343 1204 14-15 -0.17611031977762792 1204 16-17 -0.09840141644153988 1204 18-19 -0.018946309051599997 1204 20-21 -0.12230147156193993 1204 22-23 -0.1270419629140278 1204 24-25 0.023404258675213896 1204 26-27 -0.026821794014388445 1204 28-29 -0.07749084674227191 1204 30-31 -0.07023296923866695 1204 32-33 -0.09592362993367942 1204 34-35 -0.06764134409326772 1204 36-37 -0.20593900616597693 1204 38-39 -0.1568461302239399 1204 40-41 -0.13786232329415782 1204 42-43 -0.09872033074980635 1204 44-45 -0.06110301500431348 1204 46-47 -0.07657001575748978 1204 48-49 -0.04784908685334699 1204 50-51 0.04733693492676849 1204 52-53 -0.11413224177812253 1204 54-55 -0.1950500187651656 1204 56-57 -0.1064801104911055 1204 58-59 -0.14036828511675736 1204 60-61 -0.21302631535893113 1204 62-63 -0.30401319352665723 1204 64-65 -0.06740414783514836 1204 66-67 -0.338338057287892 1204 68-69 -0.15744023202120871 1204 70-71 -0.2626436203520086 1204 72-73 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0.19374267758888664 2401 54-55 0.18288872752410867 2401 56-57 0.1339838577242034 2401 58-59 0.14761776232008827 2401 60-61 0.14339878061117872 2401 62-63 0.25587802769207 2401 64-65 0.19759312152076802 2401 66-67 0.20489913406047577 2401 68-69 0.10489017266613132 2401 70-71 0.3445594514805066 2401 72-73 0.18824973133499157 2401 74-75 0.22832209460919728 2401 76-77 0.2235563854568312 2401 78-79 0.24167720344986776 2401 80-81 0.14495280694745816 2401 82-83 0.2367758677147691 2401 84-85 0.1266377129512506 2401 86-87 -0.06653138928802349 2401 88-89 0.4950285180556264 2401 90-91 -8.723152492393638E-4 2402 1 0.3034649773486464 2402 2 0.2057799105410325 2402 3 0.21849684933813052 2402 4 0.37393515505953445 2402 5 0.2022242576127553 2402 6 0.22460438050612908 2402 7 0.1274341621364954 2402 8 0.2127105698457612 2402 9 0.20294256407981237 2402 10-11 0.23404082404382365 2402 12-13 0.18094273598207167 2402 14-15 0.18831784286363273 2402 16-17 0.08865696045150173 2402 18-19 0.06890182425142655 2402 20-21 0.07339266010200873 2402 22-23 0.03632156951987753 2402 24-25 0.0961179767113407 2402 26-27 0.060787880028151164 2402 28-29 0.13541754702369957 2402 30-31 0.09047738109844516 2402 32-33 0.05784966763242494 2402 34-35 0.0920886993460428 2402 36-37 0.07219416343876617 2402 38-39 0.010204850251923858 2402 40-41 0.08455545866736713 2402 42-43 0.12705525975134435 2402 44-45 0.08441117212743166 2402 46-47 0.07674719685504527 2402 48-49 0.03726555926289166 2402 50-51 0.12267392375063224 2402 52-53 0.12178257290128158 2402 54-55 0.12453837816218538 2402 56-57 0.12682925583114013 2402 58-59 0.08132339523725562 2402 60-61 0.1642734129728538 2402 62-63 0.09500512761390212 2402 64-65 0.060329385188381934 2402 66-67 0.03200012773377381 2402 68-69 0.10757841644840482 2402 70-71 0.08851515637162777 2402 72-73 0.07663977732586602 2402 74-75 0.0648437607459087 2402 76-77 0.26898726643477744 2402 78-79 0.2143560250399048 2402 80-81 -0.10020151048727399 2402 82-83 -0.003954406005192368 2402 84-85 0.5321946911842801 2402 86-87 0.09495009219346429 2402 88-89 -0.03365905453438245 2402 90-91 0.57055625617933 2403 1 -0.19833630050855788 2403 2 -0.1362851777862062 2403 3 0.01548051667522543 2403 4 -0.033192008695202446 2403 5 0.11208160933043132 2403 6 0.11053421952196629 2403 7 0.1142486870595576 2403 8 0.11313039682615766 2403 9 0.0021051314258002662 2403 10-11 0.06932183954450721 2403 12-13 0.11086353629870871 2403 14-15 0.10958513876592946 2403 16-17 0.09167541345268404 2403 18-19 0.1059335323959516 2403 20-21 0.04218027086443499 2403 22-23 0.02611466489067027 2403 24-25 0.020490558516016222 2403 26-27 0.0254685974698603 2403 28-29 0.04128411918385666 2403 30-31 0.11075286352588876 2403 32-33 0.0033793709957947726 2403 34-35 -0.04451611283251822 2403 36-37 0.02267339641851862 2403 38-39 0.0770235773580481 2403 40-41 -0.026261448232489215 2403 42-43 0.0992585145039584 2403 44-45 0.027449747373538003 2403 46-47 0.09352987875237062 2403 48-49 0.06203913015735907 2403 50-51 0.07316476641600644 2403 52-53 0.07394972847208692 2403 54-55 0.06458519753515901 2403 56-57 0.09278580308986761 2403 58-59 -0.05693116510484941 2403 60-61 0.26518221958376387 2403 62-63 0.16029039910807086 2403 64-65 0.2699330749439923 2403 66-67 0.1505455049860629 2403 68-69 0.1118870898478832 2403 70-71 0.05172666729274056 2403 72-73 0.19964548246117886 2403 74-75 0.11096354986226942 2403 76-77 0.2712878540753323 2403 78-79 0.2548968112021015 2403 80-81 0.15070488226158574 2403 82-83 0.12265837564452653 2403 84-85 -0.42982433016027954 2403 86-87 0.40591783412894245 2403 88-89 -0.018144032122805243 2403 90-91 0.57055625617933 2404 1 -0.38427974918918295 2404 2 -0.2614425221986423 2404 3 -0.33682713702034306 2404 4 -0.25629094068380454 2404 5 -0.07758501357582759 2404 6 -0.12816497657357218 2404 7 -0.0692786078521408 2404 8 -0.07470890722888868 2404 9 -0.009053772916523428 2404 10-11 0.08295771175381361 2404 12-13 0.17925333343060856 2404 14-15 0.11745766855518269 2404 16-17 0.09858022684028356 2404 18-19 0.08282755369440054 2404 20-21 0.13523742453297416 2404 22-23 0.0348689991017892 2404 24-25 0.04842787945227656 2404 26-27 -0.024124334194404184 2404 28-29 0.06252851077258725 2404 30-31 0.07028349452524907 2404 32-33 0.12105752890219179 2404 34-35 0.08724642210381717 2404 36-37 0.05487022567712074 2404 38-39 0.029223715073911194 2404 40-41 0.0842515295261137 2404 42-43 0.12702290594636167 2404 44-45 0.06715160669892839 2404 46-47 0.052150423506738264 2404 48-49 0.02141622344547045 2404 50-51 0.004371592153866288 2404 52-53 0.12369419661492742 2404 54-55 0.1335239263477419 2404 56-57 0.16367125229831458 2404 58-59 0.09735593243414087 2404 60-61 0.010508439674040915 2404 62-63 0.1763118763820728 2404 64-65 0.14492369449918385 2404 66-67 0.08254687290394713 2404 68-69 0.09537488004549743 2404 70-71 0.21102331871661306 2404 72-73 0.09207030883324308 2404 74-75 0.4144469079298929 2404 76-77 0.13496449428737378 2404 78-79 0.26462083072028264 2404 80-81 0.3413588634770406 2404 82-83 0.3672102534683077 2404 84-85 0.29890886619200785 2404 86-87 0.5882834255267895 2404 88-89 0.6901504692751388 2404 90-91 0.17055625617933146 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 19.0 31 371.0 32 2663.0 33 4863.0 34 6512.0 35 8122.0 36 11610.0 37 17810.0 38 47001.0 39 266353.0 40 297705.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.846951472418084 13.701594962482561 29.7708231213001 29.680630443799256 2 27.885898903605028 17.807934044353544 28.059053591979378 26.247113460062053 3 27.62610761283511 19.321292560612342 20.849289242487085 32.203310584065456 4 26.794453937912216 20.56410805560541 19.705925381846043 32.935512624636324 5 27.025213075144528 21.171773783650487 19.933366413837103 31.869646727367883 6 27.39126041244048 23.550101126798374 22.868532145652754 26.19010631510839 7 25.908067369602232 23.34302119515134 22.75526409855376 27.99364733669266 8 24.937823232467963 24.595756746688306 23.3277880756347 27.138631945209035 9 24.873572649160142 24.747635472958194 23.73727242699791 26.64151945088375 10-11 24.59025170844714 23.695041996654748 23.661785532759502 28.052920762138612 12-13 24.847781922057706 23.406593075114362 23.513300323213613 28.23232467961432 14-15 25.124919121184746 22.95140936520122 23.43472155818222 28.488949955431814 16-17 25.219409709077585 22.89816885837573 23.185034138778242 28.697387293768443 18-19 25.344215713038192 23.05072628799042 23.127495177435677 28.477562821535706 20-21 25.317142990728914 23.147705454814194 23.14054136395241 28.394610190504487 22-23 25.10365308304765 22.806242260896582 23.390756663735672 28.699347992320096 24-25 25.084498566427715 23.152607201193312 23.395960056045816 28.366934176333164 26-27 25.307037852039656 23.233448310707374 23.278393554429748 28.181120282823223 28-29 25.4343324349312 22.768008639139463 23.270927817636935 28.526731108292395 30-31 25.65286147474411 23.014493453097817 23.21893567875045 28.113709393407625 32-33 25.71747480026763 23.04518543155199 23.193100714028116 28.04423905415226 34-35 25.602593031799124 22.702186276905525 23.399807287916595 28.295413403378756 36-37 25.694519968581957 22.956057898961653 23.379769888742157 27.969652243714233 38-39 25.80064844088112 23.010390458236447 23.257315131998794 27.93164596888364 40-41 25.808328680165747 22.78092887021091 23.419147539698866 27.991594909924473 42-43 25.906299701920137 22.974268229742684 23.38020454808776 27.739227520249415 44-45 26.010403432107065 23.01015844438494 23.335593297591714 27.64384482591628 46-47 25.94583596453247 22.775246174784407 23.43427626412318 27.844641596559942 48-49 26.031229544485395 23.07314145098689 23.317996507114785 27.577632497412928 50-51 26.139789940249603 22.963804790485337 23.344618357603352 27.551786911661708 52-53 26.135512697806583 22.731412834512778 23.44321072755134 27.6898637401293 54-55 26.21949086544038 22.926520324694334 23.291976353061823 27.562012456803465 56-57 26.381658938691395 22.90757341576507 23.12457496136012 27.58619268418341 58-59 26.278382462872234 22.67704107255681 23.317881269830984 27.72669519473997 60-61 26.415414894757067 22.82751031780674 23.283872333640883 27.473202453795302 62-63 26.66065185519341 22.749639408095064 23.072836596962123 27.5168721397494 64-65 26.610820812069214 22.757863489417073 23.027604389050417 27.603711309463296 66-67 26.597532211847724 22.932106586616246 22.7547221301729 27.715639071363128 68-69 26.898333851050516 22.691769197178218 22.85473756240743 27.555159389363837 70-71 26.940373608403046 22.760865463236684 22.759293037297944 27.53946789106233 72-73 26.713444662494908 22.99157008676763 22.793473297046447 27.501511953691015 74-75 27.2309114384647 22.780826339573917 22.43676272081695 27.551499501144434 76-77 27.304048993259023 22.521038022646163 22.280477596158082 27.894435387936735 78-79 27.504067055236536 22.50940001508312 22.091382152361046 27.8951507773193 80-81 27.303685358171588 22.620938433790077 21.898134438851052 28.177241769187283 82-83 27.808231097189594 21.995997563734445 21.75585138780127 28.43991995127469 84-85 28.808831801987623 21.327114194637165 21.08803675229702 28.77601725107819 86-87 28.577248192280276 21.02386529517602 20.02580031910921 30.373086193434496 88-89 29.113851448236304 19.650129050759965 20.619443647834814 30.616575853168914 90-91 25.519875512802376 21.191116140896874 22.80379120101853 30.485217145282217 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.5 9 4.5 10 6.5 11 8.0 12 8.0 13 13.0 14 21.0 15 26.0 16 37.5 17 61.5 18 87.0 19 123.0 20 185.5 21 257.5 22 338.5 23 473.5 24 679.5 25 873.0 26 1094.0 27 1442.0 28 1841.0 29 2283.0 30 2857.5 31 3531.5 32 4249.5 33 5014.5 34 6040.5 35 7264.0 36 8690.5 37 10404.0 38 12379.5 39 14725.0 40 17277.0 41 20069.5 42 22993.0 43 26048.5 44 29491.5 45 33062.0 46 36782.5 47 40736.0 48 44307.0 49 47420.0 50 50386.0 51 52990.0 52 54817.5 53 56075.0 54 56713.0 55 56363.5 56 55506.0 57 54334.5 58 52661.5 59 50212.0 60 47455.0 61 44701.0 62 42076.0 63 39204.5 64 35575.5 65 32038.5 66 28721.5 67 25709.0 68 22629.5 69 19328.5 70 16482.0 71 13806.5 72 11232.5 73 8830.5 74 6735.5 75 5303.5 76 4087.0 77 2941.5 78 2190.5 79 1636.5 80 1146.0 81 799.0 82 550.0 83 355.0 84 241.5 85 177.0 86 111.0 87 63.5 88 41.5 89 26.5 90 15.0 91 10.5 92 6.0 93 2.5 94 1.0 95 1.0 96 2.0 97 3.0 98 2.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.032918620452499E-4 2 0.005731272689429874 3 6.032918620452499E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 13864.0 32-33 29485.0 34-35 32138.0 36-37 33558.0 38-39 34688.0 40-41 35691.0 42-43 35774.0 44-45 35883.0 46-47 35308.0 48-49 33620.0 50-51 32603.0 52-53 31417.0 54-55 29373.0 56-57 27118.0 58-59 25318.0 60-61 23252.0 62-63 21374.0 64-65 19807.0 66-67 17561.0 68-69 16038.0 70-71 14703.0 72-73 13249.0 74-75 11809.0 76-77 10524.0 78-79 9541.0 80-81 8600.0 82-83 7673.0 84-85 6731.0 86-87 6224.0 88-89 5380.0 90-91 4725.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.85370802572658 #Duplication Level Percentage of deduplicated Percentage of total 1 99.85840824023 99.71232340333735 2 0.13699184553742524 0.2735828749239899 3 0.0042852870012531 0.012837053910887053 4 3.1462723132833024E-4 0.0012566678277600729 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 2 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 3 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 4 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 5 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 6 0.001960698551647062 0.0 1.5082296551131248E-4 0.0 0.0 7 0.0021115215171583746 0.0 1.5082296551131248E-4 0.0 0.0 8 0.005429626758407249 0.0 1.5082296551131248E-4 0.0 0.0 9 0.005429626758407249 0.0 1.5082296551131248E-4 0.0 0.0 10-11 0.005429626758407249 7.541148275565624E-5 1.5082296551131248E-4 0.0 0.0 12-13 0.005429626758407249 1.5082296551131248E-4 1.5082296551131248E-4 0.0 0.0 14-15 0.005429626758407249 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 16-17 0.005429626758407249 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 18-19 0.005429626758407249 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 20-21 0.005505038241162906 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 22-23 0.006032918620452499 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 24-25 0.006334564551475124 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 26-27 0.006937856413520374 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 28-29 0.007239502344542999 1.5082296551131248E-4 1.5082296551131248E-4 0.0 1.5082296551131248E-4 30-31 0.007767382723832593 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 32-33 0.007918205689343906 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 34-35 0.008370674585877843 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 36-37 0.009124789413434406 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 38-39 0.00942643534445703 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 40-41 0.009652669792723998 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 42-43 0.010105138689257935 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 44-45 0.01040678462028056 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 46-47 0.011010076482325812 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 48-49 0.011688779827126718 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 50-51 0.012065837240904997 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 52-53 0.01221666020641631 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 54-55 0.012292071689171968 1.5082296551131248E-4 1.5082296551131248E-4 0.0 3.0164593102262495E-4 56-57 0.012593717620194591 1.5082296551131248E-4 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