##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-16A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 634309 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.2260049912582 40.0 40.0 40.0 40.0 40.0 2 39.13420746040179 40.0 40.0 40.0 40.0 40.0 3 39.11814588788745 40.0 40.0 40.0 40.0 40.0 4 39.049163735655654 40.0 40.0 40.0 40.0 40.0 5 39.18879914994112 40.0 40.0 40.0 40.0 40.0 6 39.241580996012985 40.0 40.0 40.0 40.0 40.0 7 39.27253751720376 40.0 40.0 40.0 40.0 40.0 8 39.30948480945407 40.0 40.0 40.0 40.0 40.0 9 39.332527206771466 40.0 40.0 40.0 40.0 40.0 10-11 39.33701397899131 40.0 40.0 40.0 40.0 40.0 12-13 39.34374256080238 40.0 40.0 40.0 40.0 40.0 14-15 39.34532538557706 40.0 40.0 40.0 40.0 40.0 16-17 39.48365228934163 40.0 40.0 40.0 40.0 40.0 18-19 39.49087905106187 40.0 40.0 40.0 40.0 40.0 20-21 39.50163721466982 40.0 40.0 40.0 40.0 40.0 22-23 39.504683048797986 40.0 40.0 40.0 40.0 40.0 24-25 39.50594741679528 40.0 40.0 40.0 40.0 40.0 26-27 39.49187541088018 40.0 40.0 40.0 40.0 40.0 28-29 39.48973765152316 40.0 40.0 40.0 40.0 40.0 30-31 39.48052995507894 40.0 40.0 40.0 40.0 40.0 32-33 39.47972683010751 40.0 40.0 40.0 40.0 40.0 34-35 39.47606321998265 40.0 40.0 40.0 40.0 40.0 36-37 39.47193439474394 40.0 40.0 40.0 40.0 40.0 38-39 39.46357007071737 40.0 40.0 40.0 40.0 40.0 40-41 39.44898886633791 40.0 40.0 40.0 40.0 40.0 42-43 39.44476011771003 40.0 40.0 40.0 40.0 40.0 44-45 39.42615835529511 40.0 40.0 40.0 40.0 40.0 46-47 39.42398147816979 40.0 40.0 40.0 40.0 40.0 48-49 39.42218181441697 40.0 40.0 40.0 40.0 40.0 50-51 39.41981670787618 40.0 40.0 40.0 40.0 40.0 52-53 39.40594353134749 40.0 40.0 40.0 40.0 40.0 54-55 39.38912012912182 40.0 40.0 40.0 40.0 40.0 56-57 39.374542174651225 40.0 40.0 40.0 40.0 40.0 58-59 39.3709013599167 40.0 40.0 40.0 40.0 40.0 60-61 39.342685166471156 40.0 40.0 40.0 40.0 40.0 62-63 39.31574366433468 40.0 40.0 40.0 40.0 40.0 64-65 39.32024357797586 40.0 40.0 40.0 40.0 40.0 66-67 39.31598294688981 40.0 40.0 40.0 40.0 40.0 68-69 39.315678608480084 40.0 40.0 40.0 40.0 40.0 70-71 39.30644668353162 40.0 40.0 40.0 40.0 40.0 72-73 39.30204408360258 40.0 40.0 40.0 40.0 40.0 74-75 39.29897392995777 40.0 40.0 40.0 40.0 40.0 76-77 39.2854640355244 40.0 40.0 40.0 40.0 40.0 78-79 39.27129592065077 40.0 40.0 40.0 40.0 40.0 80-81 39.259235522967145 40.0 40.0 40.0 40.0 40.0 82-83 39.24526453718407 40.0 40.0 40.0 40.0 40.0 84-85 39.25031263837616 40.0 40.0 40.0 40.0 40.0 86-87 39.2681567626401 40.0 40.0 40.0 40.0 40.0 88-89 39.32167168885339 40.0 40.0 40.0 40.0 40.0 90-91 39.437516526679964 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13281199842804625 1101 2 0.19658087522037704 1101 3 0.17266866966376426 1101 4 0.008881629958708004 1101 5 6.931223280517429E-4 1101 6 -0.04768050279584202 1101 7 0.1420206198325431 1101 8 -0.0038852545798420124 1101 9 0.1479198317223478 1101 10-11 0.10824132102036543 1101 12-13 -5.954136448664826E-4 1101 14-15 0.067637180747262 1101 16-17 0.10372544987455257 1101 18-19 0.10892848883970174 1101 20-21 0.059999758663416 1101 22-23 0.039576109329594544 1101 24-25 0.10156407590376659 1101 26-27 0.093111702391802 1101 28-29 0.12735275260377676 1101 30-31 0.12705645204501081 1101 32-33 0.12971544239310617 1101 34-35 0.18138675832152984 1101 36-37 0.10747761204279982 1101 38-39 0.10584500462002921 1101 40-41 0.11674126560050979 1101 42-43 0.038247728014255244 1101 44-45 0.10551303570836268 1101 46-47 0.06780120119851318 1101 48-49 -0.025708553694812508 1101 50-51 0.03700514722097381 1101 52-53 0.11755418047250288 1101 54-55 0.21696907518925457 1101 56-57 -0.010755381609413917 1101 58-59 0.017016067843094618 1101 60-61 -0.058458838736491714 1101 62-63 -0.03268103651658549 1101 64-65 -0.25365086442015183 1101 66-67 0.012046217557752925 1101 68-69 0.040446630567771535 1101 70-71 -0.2241297898113359 1101 72-73 0.04060728935895952 1101 74-75 -0.02839009663104264 1101 76-77 -0.08055411506169463 1101 78-79 -0.36318173181945923 1101 80-81 -0.14845446372408588 1101 82-83 0.12159442952241051 1101 84-85 0.4967455937303171 1101 86-87 0.007199404545971788 1101 88-89 -0.24655609773317622 1101 90-91 0.5610310511820984 1102 1 0.3799259248240219 1102 2 -0.0513652845039303 1102 3 0.19682698030176482 1102 4 -0.04591064695695479 1102 5 -0.01623407363423013 1102 6 0.13425409676736422 1102 7 -0.09371330913281639 1102 8 -0.15702232893676893 1102 9 -0.1037114940314865 1102 10-11 -0.0986940590814811 1102 12-13 -0.006975869162090476 1102 14-15 -0.052130715212307166 1102 16-17 0.047475485062768996 1102 18-19 0.005045705781846266 1102 20-21 0.09549200215666076 1102 22-23 -0.003638604309976756 1102 24-25 0.05167614077906535 1102 26-27 0.10930903074879694 1102 28-29 0.011304827019564812 1102 30-31 0.046810938094864696 1102 32-33 0.012079015146923666 1102 34-35 -0.05115448586499838 1102 36-37 0.015249936947796527 1102 38-39 -0.024430168899385762 1102 40-41 -0.1338016217554241 1102 42-43 0.128253994989997 1102 44-45 0.07232165150097813 1102 46-47 0.07550556651602847 1102 48-49 -0.11364118886888264 1102 50-51 0.04519422628144554 1102 52-53 -0.0025656994530720567 1102 54-55 0.013386444284890331 1102 56-57 -0.08822023014371183 1102 58-59 -9.912777867171485E-4 1102 60-61 -0.05869391973012483 1102 62-63 -0.15609376385968687 1102 64-65 -0.1899305036148533 1102 66-67 -0.21435577770179748 1102 68-69 -0.2002953675245891 1102 70-71 -0.0775593212933714 1102 72-73 0.039898937498328735 1102 74-75 -0.2298135446183096 1102 76-77 -0.09608900905610795 1102 78-79 0.026537568294621394 1102 80-81 -0.11894995198714042 1102 82-83 -0.08943005650208136 1102 84-85 -0.04115207846316338 1102 86-87 -0.010582226826599594 1102 88-89 -0.4516128296191795 1102 90-91 -0.659802282151233 1103 1 0.3099037343384765 1103 2 0.1662697286063377 1103 3 -0.002868246854752954 1103 4 -0.1801839904157987 1103 5 0.15665121843134244 1103 6 0.05921045883749798 1103 7 0.021993574955274653 1103 8 0.164571960476529 1103 9 -0.056088344393053546 1103 10-11 -0.10386361101502217 1103 12-13 -0.04899321350755059 1103 14-15 -0.10798222786794298 1103 16-17 -0.1208044626472784 1103 18-19 -0.042414226664377 1103 20-21 -0.07913830334663174 1103 22-23 0.07534844158478649 1103 24-25 -0.060182041616151594 1103 26-27 -0.05938903507709625 1103 28-29 -0.054510994469190166 1103 30-31 0.07022253004180357 1103 32-33 -0.0331531350514922 1103 34-35 0.013902153671402573 1103 36-37 0.029319438889459093 1103 38-39 -0.0028871758290662797 1103 40-41 -0.1818170537388113 1103 42-43 -0.09644929376857903 1103 44-45 -0.06480945052214082 1103 46-47 0.07134520138697553 1103 48-49 -0.09575034266566718 1103 50-51 0.034986214700573726 1103 52-53 -0.09561420899718343 1103 54-55 -0.011730601280362407 1103 56-57 -0.07277911492176514 1103 58-59 -0.08415921613838151 1103 60-61 -0.07018039769217665 1103 62-63 -0.12905294968832237 1103 64-65 -0.105246728579516 1103 66-67 -0.3165157080782848 1103 68-69 -0.4453487783905601 1103 70-71 -0.3949716964962917 1103 72-73 -0.12387080031567166 1103 74-75 -0.3509394143210187 1103 76-77 -0.34674701928469887 1103 78-79 -0.3453564385249521 1103 80-81 -0.03592401611053475 1103 82-83 -0.11369107700353709 1103 84-85 -0.055631851563624934 1103 86-87 -0.33885544150887625 1103 88-89 0.13134456386453053 1103 90-91 0.5610310511820984 1104 1 0.30563316314957945 1104 2 0.24762410975481686 1104 3 0.17936312659144704 1104 4 0.32551264650938805 1104 5 0.04671451439718055 1104 6 -0.046760714806978854 1104 7 0.01570973449161528 1104 8 0.049144522056643325 1104 9 0.13938179973872167 1104 10-11 0.021935214577901263 1104 12-13 -0.08068695254514324 1104 14-15 -0.008081366127676404 1104 16-17 0.0031946222303247396 1104 18-19 -9.591820521634986E-4 1104 20-21 0.018688280959018755 1104 22-23 0.08748906539322121 1104 24-25 0.10701082042915289 1104 26-27 0.011685471138100922 1104 28-29 0.05576125444410707 1104 30-31 0.019494245133699906 1104 32-33 0.03258597379931416 1104 34-35 -0.011617140402044868 1104 36-37 0.018750790221673697 1104 38-39 0.12881823169460205 1104 40-41 0.12241387184676 1104 42-43 -0.07854255460602388 1104 44-45 0.015603789319889927 1104 46-47 0.0032899373868673365 1104 48-49 0.048934910363499284 1104 50-51 -0.05259101232571339 1104 52-53 0.06937658162895133 1104 54-55 -0.12972135245566108 1104 56-57 -0.05700653069732908 1104 58-59 0.03142997353497634 1104 60-61 0.06462929658665217 1104 62-63 -0.09974692518475337 1104 64-65 0.0373160206036971 1104 66-67 -0.05777890252291229 1104 68-69 -0.1141267179819323 1104 70-71 -0.10156802914460883 1104 72-73 -0.24100733041509415 1104 74-75 -0.235622874079624 1104 76-77 -0.6722537599446099 1104 78-79 -0.3274909158184087 1104 80-81 -0.1213563690994448 1104 82-83 -0.18432100007723307 1104 84-85 -0.2373055633613177 1104 86-87 0.2739951781910577 1104 88-89 -0.1505402054475553 1104 90-91 -0.10563561548457301 1201 1 0.33346480705572645 1201 2 0.11351873024560177 1201 3 0.3175681192325115 1201 4 0.25521538998672355 1201 5 0.3718128216819423 1201 6 0.14992299593752278 1201 7 0.1757596636679608 1201 8 0.12444207127711593 1201 9 0.05668706235319121 1201 10-11 0.10009613199886047 1201 12-13 0.06144341736496273 1201 14-15 0.008942122986958623 1201 16-17 -0.058599186680872606 1201 18-19 0.12424449644487368 1201 20-21 -0.05537117956433946 1201 22-23 -0.1053448624777431 1201 24-25 -0.009851661841885573 1201 26-27 -0.11586532115108383 1201 28-29 -0.04607638077882825 1201 30-31 0.04912540440602697 1201 32-33 -0.003360424239012616 1201 34-35 -0.02359461963595777 1201 36-37 0.020697898551176763 1201 38-39 -0.05693809772260039 1201 40-41 0.03601457554079701 1201 42-43 -0.005588383341823544 1201 44-45 0.09572052861870617 1201 46-47 0.023658539002255452 1201 48-49 -0.01796961729273505 1201 50-51 0.08626253500543157 1201 52-53 0.011396036609831128 1201 54-55 0.11107977103737454 1201 56-57 -0.020740541668672563 1201 58-59 0.10679258582026563 1201 60-61 0.09288412052752193 1201 62-63 -0.06846549637008792 1201 64-65 0.021301755777791698 1201 66-67 0.007601629265494125 1201 68-69 -0.06926100971925564 1201 70-71 -0.21405664760609255 1201 72-73 -0.04695278031662298 1201 74-75 -0.15645164555240854 1201 76-77 -0.07394495529353406 1201 78-79 -0.09763143467243651 1201 80-81 -0.2051944063320832 1201 82-83 -0.33611663336106545 1201 84-85 -0.34412477851496703 1201 86-87 -0.05158437923780923 1201 88-89 0.12287934487299879 1201 90-91 -0.054353564202514804 1202 1 0.1451574450491222 1202 2 0.3110868473989612 1202 3 0.2001744289898184 1202 4 0.18462928049635963 1202 5 0.12300079124536012 1202 6 0.1258044433177119 1202 7 0.09010390936458634 1202 8 0.12117356946061619 1202 9 -0.035959654168813415 1202 10-11 0.08762117204290121 1202 12-13 0.04549077859716988 1202 14-15 -0.10967814555180411 1202 16-17 -0.02067406225393853 1202 18-19 0.014081209321858523 1202 20-21 -0.06643174698587018 1202 22-23 -0.08818077736673757 1202 24-25 -0.06165278703610255 1202 26-27 0.02237129897592638 1202 28-29 0.03146775667983803 1202 30-31 0.13625785928498857 1202 32-33 0.0028746069791907303 1202 34-35 -0.007860876967171748 1202 36-37 0.01857327298149869 1202 38-39 0.012489453407887652 1202 40-41 0.13282884066273937 1202 42-43 0.03439748109780538 1202 44-45 0.034559555126939756 1202 46-47 0.1077879414286258 1202 48-49 0.015050537525091556 1202 50-51 0.029912465412650135 1202 52-53 -0.09117228262144295 1202 54-55 -0.0178596123982544 1202 56-57 0.06693025456851842 1202 58-59 0.023930072416540327 1202 60-61 -0.11003688023824765 1202 62-63 -0.13129118563369246 1202 64-65 -0.12467133549981213 1202 66-67 -0.10209962948506757 1202 68-69 -0.10217971682723004 1202 70-71 -0.043134636277834204 1202 72-73 -0.21454221696054532 1202 74-75 -0.1312683698025907 1202 76-77 -0.03974578171489185 1202 78-79 -0.03354527585703693 1202 80-81 -0.07359174255263667 1202 82-83 -0.25062535808815767 1202 84-85 -0.3747133414255117 1202 86-87 -0.4400676530497236 1202 88-89 0.4749978146385274 1202 90-91 -0.010397520246471004 1203 1 0.2720126456665497 1203 2 0.07473180848145944 1203 3 0.1058395954763185 1203 4 0.21659647495329892 1203 5 -0.10837827074047368 1203 6 0.08727476590583905 1203 7 0.05976015637163812 1203 8 0.10233134091355822 1203 9 0.11950591061917493 1203 10-11 0.005587558214962485 1203 12-13 0.09916484566591777 1203 14-15 0.05372853898990115 1203 16-17 0.12047944873886962 1203 18-19 0.02376602546281248 1203 20-21 0.06163201561030007 1203 22-23 0.06972424443591052 1203 24-25 -0.012089720415296767 1203 26-27 -0.03289872271719929 1203 28-29 0.03152444828651113 1203 30-31 -0.06649108236432255 1203 32-33 0.04714818431362744 1203 34-35 0.09020480240958051 1203 36-37 0.04792181353518998 1203 38-39 0.017710401102156936 1203 40-41 0.02772275488544551 1203 42-43 -0.030497782778653004 1203 44-45 -0.07988839114386082 1203 46-47 0.08408259686341069 1203 48-49 -0.045673203992691924 1203 50-51 0.010303609794043211 1203 52-53 0.02908836422052019 1203 54-55 -0.03035745826602465 1203 56-57 0.093325610997951 1203 58-59 0.06342456698367727 1203 60-61 0.03541099711490858 1203 62-63 0.09253125511240512 1203 64-65 0.10201590992284792 1203 66-67 0.032881821186180105 1203 68-69 0.13408570649681906 1203 70-71 0.10850563649027833 1203 72-73 0.06878868170478114 1203 74-75 0.1167242214967672 1203 76-77 0.047583626339580576 1203 78-79 0.11964463532867597 1203 80-81 -0.024945810806450197 1203 82-83 -0.44886695211328487 1203 84-85 -0.1037117077394285 1203 86-87 0.3750949273710731 1203 88-89 0.3659695879632423 1203 90-91 0.2851689822165824 1204 1 0.16956461797168743 1204 2 0.2090606008880016 1204 3 0.004852594411254074 1204 4 0.19882011223081264 1204 5 0.028371559156596504 1204 6 -0.03790159972577101 1204 7 -0.020134080815694233 1204 8 0.07434233740159613 1204 9 -0.05669971918189276 1204 10-11 -0.12542887348608645 1204 12-13 -0.06412831482236214 1204 14-15 -0.06040850983327317 1204 16-17 -0.034555032459302026 1204 18-19 -0.10852314790965067 1204 20-21 -0.02106306134410829 1204 22-23 0.015869897800875776 1204 24-25 0.015419277666289588 1204 26-27 -0.13553826773023303 1204 28-29 -0.13003455116884055 1204 30-31 -0.12012239617311593 1204 32-33 -0.047597988242060296 1204 34-35 -0.011530161027216934 1204 36-37 -0.07336798656365318 1204 38-39 -0.08649593371305286 1204 40-41 -0.08031596425669818 1204 42-43 -0.09066520761280117 1204 44-45 -0.08835190760071043 1204 46-47 -0.21276998619960352 1204 48-49 -0.032671090098077116 1204 50-51 -0.05411225685406862 1204 52-53 -0.15628705522060216 1204 54-55 -0.11322240989979804 1204 56-57 -0.04343159732316337 1204 58-59 -0.034689546879185684 1204 60-61 -0.04338465226457089 1204 62-63 -0.302976189106964 1204 64-65 -0.41999567571946983 1204 66-67 -0.18345463218111746 1204 68-69 -0.10439742726900647 1204 70-71 -0.35385558940503614 1204 72-73 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0.002443531414115796 2401 48-49 0.0779515368467969 2401 50-51 0.05233682218154456 2401 52-53 0.1692503729998336 2401 54-55 0.15361890440679815 2401 56-57 0.11426977851931497 2401 58-59 0.19178259375095053 2401 60-61 0.015206861010490513 2401 62-63 0.23160893818258188 2401 64-65 0.21515083645679312 2401 66-67 0.1345596278063823 2401 68-69 0.2539772205365196 2401 70-71 0.25923856375363385 2401 72-73 0.17029646869138304 2401 74-75 0.2930022649147759 2401 76-77 0.19245213412177264 2401 78-79 0.37576424038926604 2401 80-81 0.24411630990100974 2401 82-83 0.2896653001546241 2401 84-85 0.5253686791739014 2401 86-87 0.26347139027866007 2401 88-89 0.12466370509109481 2401 90-91 0.33880882895987696 2402 1 0.3267048920564406 2402 2 0.2340927940524935 2402 3 0.31107911497036866 2402 4 0.4112748054572606 2402 5 0.29986525774046413 2402 6 0.16190436934125785 2402 7 0.14917580604242886 2402 8 0.25089212964898877 2402 9 0.21893985321578668 2402 10-11 0.15610000582465489 2402 12-13 0.13812598685444044 2402 14-15 0.15481025224202938 2402 16-17 0.027326975806737153 2402 18-19 0.0023030255231688557 2402 20-21 0.04636222894507824 2402 22-23 0.047285653589902665 2402 24-25 0.006228966730340346 2402 26-27 0.09965998984628754 2402 28-29 0.08431226489736332 2402 30-31 0.06614729948100262 2402 32-33 0.08171227623604693 2402 34-35 0.09927361694239778 2402 36-37 0.11754610117435504 2402 38-39 0.14270838078101633 2402 40-41 0.09365743952510286 2402 42-43 0.07832256848528374 2402 44-45 0.05644098761803917 2402 46-47 0.06862486809822599 2402 48-49 0.09729071417103796 2402 50-51 0.09376116900477172 2402 52-53 0.08007417244191828 2402 54-55 0.08919535914138521 2402 56-57 0.20719947219301815 2402 58-59 -0.0033210948483457514 2402 60-61 0.14796084668436293 2402 62-63 0.19495026050810083 2402 64-65 0.28236078190803937 2402 66-67 0.2546064289219956 2402 68-69 0.17899706580669772 2402 70-71 0.2926933628941839 2402 72-73 0.22790254587357595 2402 74-75 0.2601113865429596 2402 76-77 0.26138343838136535 2402 78-79 0.31748905570935193 2402 80-81 0.25307285945834934 2402 82-83 0.27213833006631205 2402 84-85 0.3518480846187231 2402 86-87 0.3091965065430742 2402 88-89 -0.3556421336484803 2402 90-91 0.355902846053894 2403 1 -0.27866719035120724 2403 2 -0.09024179435399304 2403 3 -0.1658350136308684 2403 4 -0.05748196903491021 2403 5 -0.012921734382352668 2403 6 0.10789165952841273 2403 7 0.10811259626873237 2403 8 0.06857167000352149 2403 9 0.06591342956707535 2403 10-11 0.16464911533347504 2403 12-13 0.11223071524357664 2403 14-15 0.10405009762781248 2403 16-17 0.05639703743830893 2403 18-19 0.03702650906224392 2403 20-21 0.07884996361881491 2403 22-23 -0.0269571878857775 2403 24-25 0.053388753398088795 2403 26-27 -0.04597021968825743 2403 28-29 0.08205281363189698 2403 30-31 0.07247317712229062 2403 32-33 0.04538377255744308 2403 34-35 0.061976961716844414 2403 36-37 0.12733083247951527 2403 38-39 0.07100153034787837 2403 40-41 0.026134694303500794 2403 42-43 0.08894487546113794 2403 44-45 0.02576980597175549 2403 46-47 -0.02328959825847221 2403 48-49 0.10589632671641169 2403 50-51 -0.13056667369136932 2403 52-53 0.06108074700462396 2403 54-55 -0.04453722477973088 2403 56-57 0.00195976116556551 2403 58-59 0.01588621122060374 2403 60-61 -0.05157303957526693 2403 62-63 0.09292666030528807 2403 64-65 -0.010591069871423997 2403 66-67 0.10075093601830076 2403 68-69 0.1476504045123832 2403 70-71 0.23594188180106812 2403 72-73 7.599887543321415E-4 2403 74-75 0.09297136800097405 2403 76-77 0.10778873441072534 2403 78-79 0.1942206913107256 2403 80-81 -0.029693374470049605 2403 82-83 -0.03441580367658048 2403 84-85 0.3014046026364028 2403 86-87 -0.08119976385319205 2403 88-89 0.19850742050107328 2403 90-91 0.3705548607059086 2404 1 -0.39954736616870434 2404 2 -0.20444359534366185 2404 3 -0.2976432058185665 2404 4 -0.30782476900349565 2404 5 -0.09640783680189458 2404 6 -0.20064833345901434 2404 7 -0.002114213846013513 2404 8 0.02653882383715711 2404 9 -0.07561482941977715 2404 10-11 0.09695901985257649 2404 12-13 0.07746918132729519 2404 14-15 0.09501306393715225 2404 16-17 -0.03421843160599991 2404 18-19 0.0725521878729225 2404 20-21 0.02483054093964654 2404 22-23 0.027819449250380046 2404 24-25 0.01775911802636898 2404 26-27 0.050093343203407414 2404 28-29 0.04489129491124544 2404 30-31 -0.060625873202432956 2404 32-33 -0.07827580880340435 2404 34-35 0.004268922804953945 2404 36-37 0.030893919639829903 2404 38-39 0.03108614063291526 2404 40-41 0.012545657627782703 2404 42-43 -0.036336017483215244 2404 44-45 -0.04216190475267467 2404 46-47 -0.012776564428854442 2404 48-49 0.026309646709030687 2404 50-51 -0.061418681265728026 2404 52-53 -0.028933370379256473 2404 54-55 0.03710201009559455 2404 56-57 0.12124408224751448 2404 58-59 0.1730054868331692 2404 60-61 0.030131228654632025 2404 62-63 0.16127236048456695 2404 64-65 0.13031842121117165 2404 66-67 0.13530067761274722 2404 68-69 0.1800251437778968 2404 70-71 0.23408575167773193 2404 72-73 0.1267168629639528 2404 74-75 0.057071728963350665 2404 76-77 0.15575388538339752 2404 78-79 0.42255007134617983 2404 80-81 0.4202893904473868 2404 82-83 0.33031863214905144 2404 84-85 0.15543779398878144 2404 86-87 0.0647705960621181 2404 88-89 -0.13866912935226594 2404 90-91 0.2499199400709884 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 19.0 31 355.0 32 2589.0 33 4719.0 34 6179.0 35 7886.0 36 10952.0 37 17325.0 38 43834.0 39 268627.0 40 271824.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.02645606080432 13.286362697600651 30.10135630469638 29.585824936898646 2 27.344665191979935 18.010465756102793 28.47164959425093 26.173219457666345 3 27.175340491846956 19.38710679281038 21.42556475375333 32.011987961589334 4 26.58057823552874 20.6475077604133 20.214438073557208 32.55747593050075 5 26.98400937082715 21.150890181283884 20.030931296891577 31.834169150997383 6 27.592230285239527 23.51582588296871 22.882538321228296 26.009405510563465 7 26.11976181955482 23.224958182841483 22.734030259699924 27.921249737903764 8 25.15540533084033 24.461106495414693 23.33499918809287 27.048488985652103 9 25.006897269311963 24.59857892604393 23.780838676417957 26.61368512822615 10-11 24.882431117956706 23.633355352044507 23.587084528203132 27.897129001795655 12-13 25.112051066593725 23.400424714137745 23.39120207974347 28.09632213952506 14-15 25.39401143606665 22.972399887121263 23.36243061347072 28.271158063341367 16-17 25.540785327025155 22.763274681582637 23.174430758510443 28.521509232881765 18-19 25.593835181276003 22.96286194898701 23.137776698738314 28.30552617099868 20-21 25.616615876489217 23.035775938856297 23.067858094398787 28.279750090255696 22-23 25.38084750492268 22.638020270877444 23.39309390218332 28.58803832201656 24-25 25.47772457903009 22.866773134229533 23.387576086733752 28.26792620000662 26-27 25.6053437677851 22.96459611955687 23.2899107532764 28.140149359381628 28-29 25.639002442027465 22.66348104788045 23.3729144628249 28.324602047267184 30-31 25.833664402782247 22.88320500450885 23.269846825201636 28.013283767507268 32-33 25.877300524720216 22.876659798488785 23.26109553734582 27.984944139445183 34-35 25.785655326046957 22.525102847917683 23.5021166283784 28.18712519765696 36-37 25.775589142224025 22.891562129090783 23.380829738133265 27.952018990551924 38-39 25.949676157997402 22.88212686750376 23.3184989443331 27.849698030165737 40-41 25.838307391670096 22.693936923991906 23.44926553862213 28.01849014571587 42-43 26.00730444134369 22.909295009547584 23.44976871885751 27.633631830251222 44-45 26.146197715479367 22.90385284901938 23.359555032411013 27.590394403090237 46-47 26.053347550552658 22.678700003847442 23.45236590285755 27.81558654274235 48-49 26.165278934709228 22.87666466327144 23.447690881974246 27.51036552004508 50-51 26.19324226669611 22.96153923359496 23.38847682518016 27.45674167452877 52-53 26.25506127517141 22.747395130378447 23.32559520596016 27.671948388489987 54-55 26.297847387238367 22.967621098143212 23.333172875649673 27.401358638968752 56-57 26.571167877430373 22.916699668294044 23.109323566963734 27.402808887311853 58-59 26.298441179923888 22.9725669767731 23.203420149320735 27.525571693982275 60-61 26.393052338344614 22.92906239118257 23.285226567349472 27.392658703123345 62-63 26.750527081895285 22.944067800144726 23.00913191540831 27.29627320255168 64-65 26.66031743096362 22.71046098225142 23.1186921807313 27.510529406053656 66-67 26.725685631152107 22.823933634618957 22.99959759796942 27.450783136259517 68-69 26.78092001878773 22.99959989098479 22.705026876888194 27.514453213339284 70-71 26.950438466844023 22.648865241238873 22.69189082668243 27.708805465234672 72-73 27.082542319996694 22.986816235540903 22.630813243160947 27.29982820130146 74-75 27.087242945282124 22.733586160953372 22.630829585185637 27.548341308578866 76-77 27.132897915728265 22.55640637156546 22.553335244256992 27.75736046844929 78-79 27.375712621835348 22.58137681603629 22.35946790902961 27.683442653098755 80-81 27.418606044268728 22.734148021685087 22.11339743013748 27.73384850390871 82-83 27.958369110861586 22.161937742382925 21.826965719623697 28.052727427131792 84-85 28.748712475645622 21.929487099936708 20.960275995582084 28.361524428835583 86-87 29.089495263678778 21.108440548564968 20.364413968613036 29.43765021914322 88-89 28.67266363925607 20.43791397911665 20.489977728285076 30.399444653342204 90-91 25.770940653447184 20.492555218403783 23.089407945804812 30.647096182344225 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.5 8 3.0 9 3.5 10 4.0 11 3.5 12 5.5 13 13.0 14 21.5 15 31.0 16 47.5 17 66.5 18 92.5 19 125.5 20 171.0 21 242.5 22 334.5 23 478.0 24 658.0 25 825.5 26 1077.5 27 1394.5 28 1711.0 29 2097.0 30 2531.0 31 2986.0 32 3624.0 33 4382.5 34 5285.0 35 6337.5 36 7526.0 37 8885.5 38 10516.5 39 12478.0 40 14701.0 41 17324.0 42 19989.5 43 22627.5 44 25649.0 45 28940.0 46 32336.0 47 35733.0 48 38726.0 49 41651.5 50 44546.5 51 47026.0 52 48772.0 53 49946.5 54 50576.5 55 50356.0 56 49526.0 57 48038.5 58 46245.0 59 44266.0 60 42233.0 61 39747.0 62 36989.0 63 34462.5 64 31695.5 65 28762.0 66 25575.5 67 22601.5 68 19834.0 69 16915.5 70 14141.5 71 11703.0 72 9563.5 73 7504.0 74 5657.5 75 4318.0 76 3369.0 77 2502.0 78 1815.0 79 1298.0 80 894.5 81 628.0 82 430.5 83 277.0 84 183.5 85 132.5 86 84.0 87 52.5 88 37.5 89 26.0 90 16.0 91 9.0 92 5.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0018918224398518702 2 0.007882593499382792 3 9.459112199259351E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.08538396089488E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7985.0 32-33 18160.0 34-35 20267.0 36-37 21907.0 38-39 24119.0 40-41 25826.0 42-43 26604.0 44-45 27626.0 46-47 27974.0 48-49 28127.0 50-51 28352.0 52-53 27705.0 54-55 27259.0 56-57 26412.0 58-59 25626.0 60-61 24561.0 62-63 23850.0 64-65 22679.0 66-67 21611.0 68-69 20516.0 70-71 19436.0 72-73 18436.0 74-75 17489.0 76-77 16281.0 78-79 15133.0 80-81 14093.0 82-83 12893.0 84-85 12214.0 86-87 11217.0 88-89 10400.0 90-91 9551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.62919516060086 #Duplication Level Percentage of deduplicated Percentage of total 1 99.64647751499116 99.27698355407479 2 0.33814698775753166 0.6737862447252886 3 0.012580818690020814 0.037602505216446556 4 0.0025485494267059607 0.010156397128389023 5 0.0 0.0 6 2.461291345899631E-4 0.0014712988550863934 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014188668298889028 0.0 0.0 0.0 0.0 2 0.0014188668298889028 0.0 0.0 0.0 0.0 3 0.0014188668298889028 0.0 0.0 0.0 1.5765186998765586E-4 4 0.0014188668298889028 0.0 0.0 0.0 1.5765186998765586E-4 5 0.0014188668298889028 0.0 0.0 0.0 1.5765186998765586E-4 6 0.0014188668298889028 0.0 0.0 0.0 1.5765186998765586E-4 7 0.0015765186998765587 0.0 0.0 0.0 1.5765186998765586E-4 8 0.004729556099629675 0.0 0.0 0.0 1.5765186998765586E-4 9 0.004887207969617332 0.0 0.0 0.0 1.5765186998765586E-4 10-11 0.004887207969617332 0.0 1.5765186998765586E-4 0.0 2.3647780498148378E-4 12-13 0.004887207969617332 0.0 2.3647780498148378E-4 0.0 3.153037399753117E-4 14-15 0.004887207969617332 0.0 3.153037399753117E-4 0.0 3.153037399753117E-4 16-17 0.004887207969617332 0.0 3.153037399753117E-4 0.0 3.153037399753117E-4 18-19 0.005202511709592643 0.0 3.153037399753117E-4 0.0 3.153037399753117E-4 20-21 0.005202511709592643 7.882593499382793E-5 3.153037399753117E-4 0.0 3.153037399753117E-4 22-23 0.0053601635795803 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 24-25 0.005754293254549439 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 26-27 0.005911945124537094 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 28-29 0.00646372666949389 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 30-31 0.006621378539481546 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 32-33 0.006779030409469202 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 34-35 0.006936682279456858 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 36-37 0.007330811954425998 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 38-39 0.007488463824413653 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 40-41 0.007803767564388965 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 42-43 0.008197897239358105 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 44-45 0.008986156589296385 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 46-47 0.009380286264265524 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 48-49 0.009537938134253179 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 50-51 0.00985324187422849 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 52-53 0.009932067809222319 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 54-55 0.01024737154919763 1.5765186998765586E-4 3.153037399753117E-4 0.0 3.153037399753117E-4 56-57 0.010405023419185287 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