##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-15C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 575688 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26380956351357 40.0 40.0 40.0 40.0 40.0 2 39.15142577229332 40.0 40.0 40.0 40.0 40.0 3 39.1432755242423 40.0 40.0 40.0 40.0 40.0 4 39.02528452911994 40.0 40.0 40.0 40.0 40.0 5 39.183293728547405 40.0 40.0 40.0 40.0 40.0 6 39.2254137657898 40.0 40.0 40.0 40.0 40.0 7 39.26715859979711 40.0 40.0 40.0 40.0 40.0 8 39.29546559942191 40.0 40.0 40.0 40.0 40.0 9 39.31629632717722 40.0 40.0 40.0 40.0 40.0 10-11 39.30022164783702 40.0 40.0 40.0 40.0 40.0 12-13 39.30673559289059 40.0 40.0 40.0 40.0 40.0 14-15 39.308868692764136 40.0 40.0 40.0 40.0 40.0 16-17 39.45544461583357 40.0 40.0 40.0 40.0 40.0 18-19 39.469205541890744 40.0 40.0 40.0 40.0 40.0 20-21 39.47339878545323 40.0 40.0 40.0 40.0 40.0 22-23 39.48149692194383 40.0 40.0 40.0 40.0 40.0 24-25 39.47812704103612 40.0 40.0 40.0 40.0 40.0 26-27 39.468243215074835 40.0 40.0 40.0 40.0 40.0 28-29 39.46147218632315 40.0 40.0 40.0 40.0 40.0 30-31 39.45172541558949 40.0 40.0 40.0 40.0 40.0 32-33 39.45288228492999 40.0 40.0 40.0 40.0 40.0 34-35 39.44800260706799 40.0 40.0 40.0 40.0 40.0 36-37 39.43872339704406 40.0 40.0 40.0 40.0 40.0 38-39 39.4226994160945 40.0 40.0 40.0 40.0 40.0 40-41 39.414526697218996 40.0 40.0 40.0 40.0 40.0 42-43 39.404824249361674 40.0 40.0 40.0 40.0 40.0 44-45 39.392007264517005 40.0 40.0 40.0 40.0 40.0 46-47 39.38571851868457 40.0 40.0 40.0 40.0 40.0 48-49 39.39722764661008 40.0 40.0 40.0 40.0 40.0 50-51 39.39426051600173 40.0 40.0 40.0 40.0 40.0 52-53 39.37599170766274 40.0 40.0 40.0 40.0 40.0 54-55 39.35076962472442 40.0 40.0 40.0 40.0 40.0 56-57 39.35024996285193 40.0 40.0 40.0 40.0 40.0 58-59 39.325308014320974 40.0 40.0 40.0 40.0 40.0 60-61 39.30805349132272 40.0 40.0 40.0 40.0 40.0 62-63 39.35631649864696 40.0 40.0 40.0 40.0 40.0 64-65 39.35292194507374 40.0 40.0 40.0 40.0 40.0 66-67 39.36516659746847 40.0 40.0 40.0 40.0 40.0 68-69 39.350674713117996 40.0 40.0 40.0 40.0 40.0 70-71 39.34151652967965 40.0 40.0 40.0 40.0 40.0 72-73 39.32729684981224 40.0 40.0 40.0 40.0 40.0 74-75 39.347837552873465 40.0 40.0 40.0 40.0 40.0 76-77 39.315466471954565 40.0 40.0 40.0 40.0 40.0 78-79 39.3255190615412 40.0 40.0 40.0 40.0 40.0 80-81 39.285752354359886 40.0 40.0 40.0 40.0 40.0 82-83 39.273237176774636 40.0 40.0 40.0 40.0 40.0 84-85 39.30122196637886 40.0 40.0 40.0 40.0 40.0 86-87 39.33341262497741 40.0 40.0 40.0 40.0 40.0 88-89 39.34444281968311 40.0 40.0 40.0 40.0 40.0 90-91 39.4094435496888 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31435474768546356 1101 2 0.14674691297756937 1101 3 0.24099662940082567 1101 4 0.09723459531083023 1101 5 0.20598973718927027 1101 6 -0.04115917332970298 1101 7 -0.06960121953616749 1101 8 -0.02193969621082914 1101 9 -0.13766291745726988 1101 10-11 0.0211560990851396 1101 12-13 -0.09804980014127551 1101 14-15 0.02881320946045207 1101 16-17 -0.03891991160660524 1101 18-19 -0.018145476166473884 1101 20-21 -0.043593015907845256 1101 22-23 0.06648426302720623 1101 24-25 0.03577170677117891 1101 26-27 0.09586248402633402 1101 28-29 0.06753443080130239 1101 30-31 0.04046460833937715 1101 32-33 0.005271133328740518 1101 34-35 -0.038457210870674885 1101 36-37 -0.007019474662001812 1101 38-39 0.1308535527253767 1101 40-41 0.18512562067626703 1101 42-43 0.13493623854433423 1101 44-45 0.06167700549464428 1101 46-47 0.09517812732388364 1101 48-49 0.19989012398342254 1101 50-51 0.11676456381339761 1101 52-53 0.10788309464178525 1101 54-55 0.1495559609454915 1101 56-57 -0.06618552233198471 1101 58-59 0.14027993695322039 1101 60-61 0.16940923472951397 1101 62-63 -0.009487302060946945 1101 64-65 0.12369566493454442 1101 66-67 0.08270277320855257 1101 68-69 0.3783795391657776 1101 70-71 0.16339290256455996 1101 72-73 -0.11093677720867845 1101 74-75 -0.21356640418143513 1101 76-77 -0.035365714048943175 1101 78-79 0.22862796245794925 1101 80-81 0.31825806036584936 1101 82-83 0.009442363931952968 1101 84-85 0.2086433528302578 1101 86-87 -0.817446105081153 1101 88-89 -0.2813351704539855 1101 90-91 -0.20164116948277666 1102 1 0.3249236325947109 1102 2 0.10524823154758423 1102 3 0.23296608351679282 1102 4 0.17385901090287348 1102 5 -0.15383788610451177 1102 6 -0.11705867181578355 1102 7 0.17977252322101833 1102 8 0.0095953270525726 1102 9 0.026208998045845533 1102 10-11 -6.219012615105157E-4 1102 12-13 -0.11196549860510885 1102 14-15 -0.06173255036728165 1102 16-17 -0.06931110149254494 1102 18-19 -0.03139610270600457 1102 20-21 -0.04622168728270992 1102 22-23 -0.07374203080021147 1102 24-25 0.07101826045089865 1102 26-27 0.008561913859836068 1102 28-29 -0.020882873037770366 1102 30-31 0.024146069374801016 1102 32-33 -0.01687732644351314 1102 34-35 0.03293642323863821 1102 36-37 -0.06957923378782027 1102 38-39 0.09461026826549102 1102 40-41 -0.05437082899424439 1102 42-43 0.017486771607487128 1102 44-45 0.06591949094566729 1102 46-47 -0.04149353499395403 1102 48-49 0.009659744793424352 1102 50-51 0.06884320141127631 1102 52-53 -0.011424692594694363 1102 54-55 -0.15997538440615244 1102 56-57 0.021999387577771756 1102 58-59 -0.09374635117985974 1102 60-61 0.32092986811037605 1102 62-63 -0.06693256190580854 1102 64-65 -0.02104925652072609 1102 66-67 -0.2121364128114891 1102 68-69 0.015945580361318434 1102 70-71 -0.3696437914285582 1102 72-73 -0.3108531621160111 1102 74-75 -0.1453868012753361 1102 76-77 0.13696173871753103 1102 78-79 -0.10348873037303008 1102 80-81 0.2545069998375027 1102 82-83 0.075173892677185 1102 84-85 0.03053518648679443 1102 86-87 -0.026403601123043074 1102 88-89 -0.2932399323587447 1102 90-91 -0.15768512552673286 1103 1 0.274304126081951 1103 2 0.24700402949315503 1103 3 0.1161811634701948 1103 4 0.036190896956036056 1103 5 0.1267932692486653 1103 6 0.017474845075035716 1103 7 0.04574731936630627 1103 8 0.19801002577120386 1103 9 0.014009485179172998 1103 10-11 0.22237926362253546 1103 12-13 0.11683890677453945 1103 14-15 0.028260036734273797 1103 16-17 0.021168618935284655 1103 18-19 0.04463905561232906 1103 20-21 0.08396998909132947 1103 22-23 0.05291263403087498 1103 24-25 0.060047170754828016 1103 26-27 -0.002133839608340793 1103 28-29 0.014826117749052514 1103 30-31 0.04233944012561608 1103 32-33 0.06288045316598101 1103 34-35 0.017452043893946723 1103 36-37 0.01160001259258081 1103 38-39 0.04767306060693244 1103 40-41 0.0663986621449979 1103 42-43 0.07365755207468538 1103 44-45 0.053570334035896394 1103 46-47 0.09884756895786495 1103 48-49 -0.12046262762996918 1103 50-51 0.0033985661437654358 1103 52-53 -0.06917827108073027 1103 54-55 0.0109694254421413 1103 56-57 0.06426107488346844 1103 58-59 0.079708451268381 1103 60-61 0.020778605683737794 1103 62-63 -0.06404912440824972 1103 64-65 0.15237234612935424 1103 66-67 -0.12691264772499977 1103 68-69 0.07095275272178014 1103 70-71 0.07652363465258105 1103 72-73 -0.055445110079496374 1103 74-75 0.4762199214833345 1103 76-77 0.2843478876942598 1103 78-79 0.14110030859366418 1103 80-81 -0.2385217313270971 1103 82-83 0.5156468525531324 1103 84-85 -0.386055635888674 1103 86-87 0.23612772719776132 1103 88-89 0.6900934009745896 1103 90-91 -0.20164116948277666 1104 1 0.3623201719026383 1104 2 0.14059749961639767 1104 3 0.08431261907067977 1104 4 0.004185062112149751 1104 5 -0.022359927580787087 1104 6 0.07667705888344756 1104 7 0.13169971819505122 1104 8 -0.006522704056223461 1104 9 -8.221731392339393E-4 1104 10-11 0.09595621598918314 1104 12-13 0.020536095538169263 1104 14-15 0.09797998567938748 1104 16-17 0.03235135466190542 1104 18-19 0.028754122166013474 1104 20-21 -0.03320311485255445 1104 22-23 -0.019379365582992136 1104 24-25 -0.06880634677652608 1104 26-27 -0.011036266860493527 1104 28-29 0.008581547693395919 1104 30-31 0.07931347292516477 1104 32-33 -0.11467055103797463 1104 34-35 -0.04190523299541837 1104 36-37 0.019388086136387983 1104 38-39 -0.05476658076744201 1104 40-41 0.056063801090651566 1104 42-43 -0.1606770848174861 1104 44-45 -0.0052042272852048654 1104 46-47 -0.05100809823284891 1104 48-49 0.12804112707246418 1104 50-51 0.040282487646031484 1104 52-53 0.028324619224370906 1104 54-55 -0.1378783051317498 1104 56-57 -0.06085690154134227 1104 58-59 -0.17199711925367467 1104 60-61 -0.15504628986950308 1104 62-63 -0.004821636885964153 1104 64-65 -0.09056200862700337 1104 66-67 -0.10217777682849771 1104 68-69 0.0268045118767688 1104 70-71 -0.05686757030701273 1104 72-73 0.18662469561092365 1104 74-75 -0.0990380931631023 1104 76-77 0.1384536800481868 1104 78-79 0.41584738892971984 1104 80-81 0.3347955280661097 1104 82-83 0.286365440855036 1104 84-85 0.7207312649181645 1104 86-87 -0.08116550588494675 1104 88-89 0.6900934009745896 1104 90-91 0.41374344590183654 1201 1 0.2631546591231455 1201 2 0.2492812109163154 1201 3 0.3342042085041683 1201 4 0.30604792947443826 1201 5 0.22419610547969882 1201 6 0.10213933649225737 1201 7 0.2554203489003086 1201 8 0.13668690665264904 1201 9 0.06867024609613992 1201 10-11 0.1711936475908331 1201 12-13 0.009428872254268583 1201 14-15 0.16870654083519554 1201 16-17 0.12379205383280123 1201 18-19 0.07686847612377079 1201 20-21 0.008848222410470896 1201 22-23 0.12041892596616321 1201 24-25 0.06338675661675097 1201 26-27 -0.010913650771470884 1201 28-29 0.03316756886111705 1201 30-31 0.09983222870083353 1201 32-33 0.012962784846720865 1201 34-35 0.04905052348311045 1201 36-37 -0.0329728459424814 1201 38-39 0.008881590492713087 1201 40-41 0.03316601917852324 1201 42-43 0.05803235406801832 1201 44-45 0.14991763931207203 1201 46-47 0.01830161014820675 1201 48-49 0.003604449276522814 1201 50-51 -0.06850480710429707 1201 52-53 -0.10337369875042413 1201 54-55 0.10761949414050775 1201 56-57 0.07575647447077216 1201 58-59 0.08476877970228003 1201 60-61 -0.11633262958377344 1201 62-63 -0.05493969428468404 1201 64-65 -0.023682443249754215 1201 66-67 -0.0068902511350046325 1201 68-69 -0.017647734855017916 1201 70-71 0.03446337230130325 1201 72-73 0.014222985981326985 1201 74-75 0.3411679131920238 1201 76-77 -0.036363357756819426 1201 78-79 0.12745027444238133 1201 80-81 -0.04486018398397107 1201 82-83 -0.32636964575159766 1201 84-85 -0.12100786551661713 1201 86-87 -0.6997232341200927 1201 88-89 0.382401093282283 1201 90-91 -1.1418121096537206 1202 1 0.028892265414981466 1202 2 0.04340671911010219 1202 3 0.09460984992904287 1202 4 0.08395983250770911 1202 5 0.08808035344036824 1202 6 0.13326222729971704 1202 7 0.05461929067148219 1202 8 -0.04408478008907224 1202 9 0.1355734786119953 1202 10-11 0.09729743526861512 1202 12-13 0.08100802219173175 1202 14-15 -0.0665792868877162 1202 16-17 -0.014632985214170446 1202 18-19 0.07356394084349205 1202 20-21 -0.04983740840101092 1202 22-23 0.040374334178324034 1202 24-25 0.0197280140313012 1202 26-27 -0.06383189559671365 1202 28-29 0.042550505212844314 1202 30-31 -0.0023362267885360666 1202 32-33 -0.09504253539298446 1202 34-35 0.007842987246675648 1202 36-37 -0.04321019822765493 1202 38-39 0.10208478002892463 1202 40-41 -0.11218395697788708 1202 42-43 -0.048948077383698774 1202 44-45 0.010254225083201618 1202 46-47 0.11069753085292433 1202 48-49 0.03333455016900899 1202 50-51 0.06740373840737846 1202 52-53 0.019791270244617465 1202 54-55 0.029721736279128663 1202 56-57 0.020321406921240737 1202 58-59 0.08817750476259789 1202 60-61 -0.07487702008995001 1202 62-63 0.13260402909229185 1202 64-65 0.10466157406818866 1202 66-67 -0.1284805342512385 1202 68-69 -0.18309566584892423 1202 70-71 0.3076941978324115 1202 72-73 -0.185018813108222 1202 74-75 -0.028643359013400982 1202 76-77 0.16485503780823052 1202 78-79 0.03678740792021529 1202 80-81 0.17463572028246688 1202 82-83 0.0030959705992614772 1202 84-85 0.1791404938191974 1202 86-87 0.43433601387189213 1202 88-89 0.6900934009745896 1202 90-91 0.41374344590183654 1203 1 0.15552892721521516 1203 2 0.2342111777986986 1203 3 0.17747933643885005 1203 4 0.27839059910996866 1203 5 -0.1602286333338796 1203 6 -0.068856926382594 1203 7 -0.05903637901006675 1203 8 0.11626944242878778 1203 9 -0.10605880147949875 1203 10-11 -0.08994502536079807 1203 12-13 -0.010806293181879312 1203 14-15 0.020610478054969406 1203 16-17 -0.03247273081590407 1203 18-19 0.049506296177504794 1203 20-21 0.09530993315438252 1203 22-23 0.015137035614543493 1203 24-25 0.031831704553084705 1203 26-27 0.022238685908348543 1203 28-29 -0.004802664351117869 1203 30-31 0.07217489833636392 1203 32-33 0.07054599876165923 1203 34-35 -0.025014527784421148 1203 36-37 0.10270428703727674 1203 38-39 0.08557361068899638 1203 40-41 -0.05359510120739941 1203 42-43 0.0035594906924387715 1203 44-45 -0.08325787298591081 1203 46-47 -0.09198473091264958 1203 48-49 -0.0014177308867147076 1203 50-51 0.014620901101935146 1203 52-53 0.0035369361398451815 1203 54-55 -0.33015098566635004 1203 56-57 -0.10862510619846688 1203 58-59 -0.06802032105765221 1203 60-61 -0.010420975448440117 1203 62-63 -0.21885035825884813 1203 64-65 -0.363302737506892 1203 66-67 -0.16888188866843734 1203 68-69 -0.17952864604880858 1203 70-71 -0.3404769503907161 1203 72-73 -0.2753856354763258 1203 74-75 -0.3874602962127085 1203 76-77 -0.044003047348354585 1203 78-79 -0.0386740570564541 1203 80-81 -0.46318426991368966 1203 82-83 -0.05105666688670851 1203 84-85 -0.12732907990942266 1203 86-87 0.3994205747011321 1203 88-89 -0.505558772938457 1203 90-91 -0.1195898874314949 1204 1 0.28383499550903935 1204 2 0.13249945820577835 1204 3 -0.005376251193226267 1204 4 0.0617477468271872 1204 5 -0.027606815470178958 1204 6 0.09191446525223768 1204 7 -0.21417007976532432 1204 8 -0.0036772911125595442 1204 9 -0.03323538756606581 1204 10-11 -0.03803938864192702 1204 12-13 -0.07758376963479208 1204 14-15 -0.14023867483337682 1204 16-17 -0.05646207752417354 1204 18-19 -0.02920753268941212 1204 20-21 -0.10990201486221451 1204 22-23 -0.10974753752476829 1204 24-25 -0.0670028486599108 1204 26-27 -0.1695594458775318 1204 28-29 -0.017847114627656424 1204 30-31 0.033041547506755364 1204 32-33 -0.013324832133534414 1204 34-35 -0.06110196203361795 1204 36-37 -0.14539229980516666 1204 38-39 -0.13272210269702356 1204 40-41 -0.034555598378567254 1204 42-43 -0.12958234611484443 1204 44-45 -0.09663279871377028 1204 46-47 -0.09110959379794537 1204 48-49 -0.15765783421317536 1204 50-51 -0.19526814470813036 1204 52-53 -0.18730104541149473 1204 54-55 0.002154654288389679 1204 56-57 0.07960318125437027 1204 58-59 -0.12663574570020586 1204 60-61 -0.24451503478517367 1204 62-63 -0.3039823084769111 1204 64-65 -0.057662885795423335 1204 66-67 -0.19809025409273318 1204 68-69 -0.15319308984878433 1204 70-71 -0.2865271131602256 1204 72-73 0.16487712709746916 1204 74-75 -0.008974247357642184 1204 76-77 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0.22033436837627107 2401 56-57 0.17317655134424115 2401 58-59 0.15902078619510007 2401 60-61 0.26155295962201563 2401 62-63 0.1998584201319602 2401 64-65 0.09205641314638058 2401 66-67 -0.044234606126416054 2401 68-69 0.11116085936632203 2401 70-71 0.19308018029794738 2401 72-73 0.14476012508543334 2401 74-75 -0.03694261230441498 2401 76-77 0.18224703331625847 2401 78-79 0.29869664377662986 2401 80-81 -0.4833578365661282 2401 82-83 -0.2145534970502041 2401 84-85 0.021138952745765494 2401 86-87 -0.060149084777656014 2401 88-89 0.154852277498172 2401 90-91 0.41374344590183654 2402 1 0.3359191207474552 2402 2 0.2530481069411863 2402 3 0.20091945756816187 2402 4 0.23132609010262684 2402 5 0.1920824367518037 2402 6 0.22554918710138594 2402 7 0.13336939723099306 2402 8 0.15443004306885655 2402 9 0.14571911189079145 2402 10-11 0.13329761744158475 2402 12-13 0.09586329393891901 2402 14-15 0.13894102131158803 2402 16-17 0.018028526891576746 2402 18-19 0.04549762521012468 2402 20-21 0.03338761375752597 2402 22-23 0.03873360964371386 2402 24-25 0.036219339211228885 2402 26-27 0.08494153143892191 2402 28-29 0.014906924299438629 2402 30-31 0.040503616309152335 2402 32-33 0.07764382692293736 2402 34-35 0.054172531630840126 2402 36-37 0.08884864939879122 2402 38-39 -0.005891782502821741 2402 40-41 0.0375452873527351 2402 42-43 0.11914489644794202 2402 44-45 -0.021663069224779008 2402 46-47 0.12076018773190356 2402 48-49 0.049726731239573496 2402 50-51 -0.054787517181850376 2402 52-53 0.021063602578131224 2402 54-55 0.05466739184183922 2402 56-57 0.09417522418912228 2402 58-59 0.1924648516114047 2402 60-61 0.1727836637424005 2402 62-63 0.026944409919195778 2402 64-65 0.2038572618845791 2402 66-67 0.18939377396294077 2402 68-69 0.1244380646148926 2402 70-71 0.27961838299405173 2402 72-73 0.08840668137339236 2402 74-75 0.3386622552689573 2402 76-77 0.3710081016271971 2402 78-79 0.27454967044862144 2402 80-81 0.1482353151585869 2402 82-83 0.5207465018861583 2402 84-85 0.235667864961151 2402 86-87 0.3943117478815523 2402 88-89 0.32810245074834654 2402 90-91 0.16374344590183654 2403 1 -0.2083685349520792 2403 2 -0.17411991438716257 2403 3 -0.14588224246733006 2403 4 -0.1085916780381524 2403 5 0.0995395679851967 2403 6 0.28324028961421277 2403 7 0.1685036948410783 2403 8 0.06375686710133266 2403 9 0.19459703653178195 2403 10-11 0.16405510270477208 2403 12-13 0.14970767663734108 2403 14-15 0.06626329229585082 2403 16-17 0.10076554940579285 2403 18-19 0.0014982138171220072 2403 20-21 0.0280766474898968 2403 22-23 0.06813626010637108 2403 24-25 0.11413290054321124 2403 26-27 0.12529504187371998 2403 28-29 0.08510904155340882 2403 30-31 0.10461988350210305 2403 32-33 0.11495618750215186 2403 34-35 0.1585499854460437 2403 36-37 0.02349861009068377 2403 38-39 0.10937791467699753 2403 40-41 0.17177473137558508 2403 42-43 0.10596359187454851 2403 44-45 0.06933970181492555 2403 46-47 0.04568577420194231 2403 48-49 0.08650078596188848 2403 50-51 0.03396298099183781 2403 52-53 0.03344653643667783 2403 54-55 -0.012514845036058375 2403 56-57 0.06166010893796425 2403 58-59 0.014427138837206144 2403 60-61 -0.01401771806218477 2403 62-63 0.22932424790916173 2403 64-65 0.11476778543659094 2403 66-67 0.2565037893026556 2403 68-69 0.28577485591996776 2403 70-71 0.2290609117251492 2403 72-73 0.22994958814123123 2403 74-75 0.4239386743224216 2403 76-77 0.12905817472661596 2403 78-79 0.05896382377972742 2403 80-81 0.14070332685489007 2403 82-83 0.24120785871114947 2403 84-85 0.30945041885471625 2403 86-87 -0.028784553503996335 2403 88-89 -0.15435104346985185 2403 90-91 -1.2370502048918155 2404 1 -0.26975955681910335 2404 2 -0.14841160558481903 2404 3 -0.29213854223392133 2404 4 -0.23682457002247048 2404 5 -0.07692989041233744 2404 6 -0.01083895278535607 2404 7 -0.04039026030583415 2404 8 -0.09798147180627836 2404 9 -0.06263169249161393 2404 10-11 0.08297181425792388 2404 12-13 0.13788971118531634 2404 14-15 0.10575572004947986 2404 16-17 -0.01688398523614154 2404 18-19 -0.0010401484732227573 2404 20-21 0.02315391488393459 2404 22-23 -0.06045705994890227 2404 24-25 -0.001703804496180794 2404 26-27 0.05180111929559672 2404 28-29 0.020981238235570743 2404 30-31 -0.013986672848190551 2404 32-33 0.10519135088312481 2404 34-35 0.09876725563289313 2404 36-37 -0.03163626876177261 2404 38-39 0.023181149479803764 2404 40-41 0.047003516487627905 2404 42-43 0.015065551787380116 2404 44-45 0.025959035667007413 2404 46-47 -0.002919181193128395 2404 48-49 0.07365993595550435 2404 50-51 0.061173363957223614 2404 52-53 0.018844903823584502 2404 54-55 0.011175673066333047 2404 56-57 0.037331387995912735 2404 58-59 0.10430473407357965 2404 60-61 0.10744359419244631 2404 62-63 0.1772974402057912 2404 64-65 0.2852550448506861 2404 66-67 0.18496164396680115 2404 68-69 -0.032049699653029506 2404 70-71 0.17160912083168256 2404 72-73 0.19567813382827381 2404 74-75 0.07545348340346436 2404 76-77 0.27440163113772087 2404 78-79 0.04986898976154919 2404 80-81 0.26214907571171864 2404 82-83 0.0562209705992629 2404 84-85 -0.3159697660096725 2404 86-87 -0.11420122017066348 2404 88-89 -0.37757577195773706 2404 90-91 0.41374344590183654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 15.0 31 360.0 32 2345.0 33 4247.0 34 5812.0 35 7243.0 36 10268.0 37 16285.0 38 43362.0 39 239735.0 40 246016.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.287082104356223 11.893371687841551 32.89281235126337 28.926733856538856 2 26.06664709489472 18.154324000840777 30.278168151631434 25.50086075263307 3 26.320909250403435 19.31705469850595 22.66299334877007 31.699042702320547 4 26.106328427898447 21.140444129459013 21.188213059851865 31.56501438279068 5 26.416565917649837 21.327177220994706 21.110393129611875 31.145863731743585 6 26.99934686844263 23.89315045649727 23.734210197190144 25.373292477869956 7 25.293561790414255 23.649789469295868 23.682793457567293 27.373855282722587 8 24.23378635649866 24.829074081794307 24.236739344922945 26.700400216784093 9 24.22892261085866 24.94962549158572 24.579633412542908 26.241818485012715 10-11 23.893324161698697 23.60167312850016 24.61628521004433 27.888717499756815 12-13 24.392466058003638 23.33859660093662 24.281468434290794 27.987468906768942 14-15 24.91141034727144 23.05198301857951 24.0083170050444 28.028289629104652 16-17 24.882922694237156 22.790730395630966 23.903485915982266 28.422860994149605 18-19 25.081728297272132 23.126328844790926 23.720834896680145 28.071107961256793 20-21 25.115079695946413 23.217263517738775 23.566324120009448 28.10133266630536 22-23 24.908544211447868 22.830508886758103 23.812464390433707 28.448482511360318 24-25 24.984540237072856 23.29239101735662 23.846944872917277 27.876123872653242 26-27 25.22060560581426 23.33868345353733 23.74654326649157 27.694167674156834 28-29 25.09136893595142 22.938987785050237 23.925199066160836 28.04444421283751 30-31 25.375899361804873 23.199452479302956 23.906000840736098 27.51864731815608 32-33 25.45511512156694 23.203530633437175 23.93472266981774 27.406631575178142 34-35 25.22894940058929 22.872260666811613 24.190289471513935 27.708500461085162 36-37 25.371855213180535 23.180859120066902 24.203442443773714 27.243843222978853 38-39 25.430572447755605 23.1806882675348 24.184006517280938 27.204732767428652 40-41 25.393874341406438 22.850006186070306 24.383705779350116 27.372413693173144 42-43 25.494198281964454 23.139552124586274 24.37857525493122 26.987674338518055 44-45 25.594117571109475 23.205381466992034 24.362112819718273 26.838388142180218 46-47 25.489669224869992 22.92922677324914 24.505992465335506 27.07511153654536 48-49 25.668460621745055 23.241204304881826 24.33771929155305 26.752615781820065 50-51 25.736953296321886 23.23508404520995 24.36315827329706 26.66480438517111 52-53 25.654121318984213 23.077249051590876 24.370291349071675 26.89833828035324 54-55 25.91532632308995 23.35818536008241 24.2143053841525 26.512182932675145 56-57 25.854656788870027 23.374933995680543 24.227494510026993 26.542914705422437 58-59 25.91157899000947 23.117977385853248 24.232987487784005 26.737456136353277 60-61 25.971781174751456 23.262478287197478 24.207749231700834 26.55799130635023 62-63 26.172054407348526 23.28017857429622 23.928634516869813 26.619132501485442 64-65 26.237820604017788 22.982807489849744 23.946580284608455 26.832791621524017 66-67 26.298984155832244 23.312310597376396 23.86254215315612 26.526163093635237 68-69 26.372862838304073 23.30320861764931 23.807789618952697 26.51613892509392 70-71 26.424120803583307 22.947248214998446 23.83919464277795 26.789436338640293 72-73 26.479264948600985 23.14840084733648 23.759860683327485 26.61247352073505 74-75 26.93966014156925 23.148340288825867 23.48112794263448 26.430871626970404 76-77 27.074557127352328 22.779198322326668 23.409245598866836 26.736998951454165 78-79 26.93489115015582 23.092474895841477 23.115931506696306 26.856702447306397 80-81 27.328218858815532 22.869469916623892 22.956869164712902 26.845442059847674 82-83 27.97603219344431 22.26240493538265 22.336861139179916 27.424701731993125 84-85 28.21144956738177 21.74943700367429 22.13820078226858 27.90091264667536 86-87 28.83041928365303 21.422508911899506 21.83670005092514 27.910371753522323 88-89 28.948247078464107 20.328324986087924 21.419031719532555 29.304396215915414 90-91 25.807770961145195 22.11315610088616 24.3626448534424 27.716428084526246 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.5 8 2.5 9 2.0 10 4.0 11 7.5 12 9.5 13 14.5 14 29.0 15 47.5 16 61.5 17 81.0 18 115.5 19 158.5 20 211.0 21 293.5 22 404.0 23 541.0 24 739.5 25 953.0 26 1190.5 27 1512.0 28 1873.5 29 2347.0 30 2960.5 31 3693.0 32 4540.5 33 5439.5 34 6636.0 35 8030.5 36 9575.0 37 11234.5 38 13228.5 39 15722.5 40 18273.0 41 21128.0 42 24099.0 43 26895.5 44 29799.5 45 32561.5 46 35470.0 47 38511.0 48 40929.0 49 42764.0 50 44353.0 51 45619.5 52 46332.5 53 46445.0 54 46024.5 55 45292.0 56 44135.5 57 42618.5 58 40890.5 59 38932.0 60 36975.5 61 34781.5 62 32573.5 63 30324.0 64 27529.0 65 24792.0 66 22157.5 67 19800.0 68 17658.0 69 15419.5 70 13162.5 71 11023.0 72 9165.5 73 7299.5 74 5557.0 75 4348.5 76 3397.0 77 2461.5 78 1811.5 79 1338.0 80 939.5 81 638.5 82 442.5 83 303.5 84 206.0 85 144.0 86 91.0 87 56.0 88 32.5 89 20.5 90 17.5 91 13.5 92 8.0 93 3.5 94 4.0 95 5.5 96 5.5 97 3.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010422312085713095 2 0.005037450841427996 3 8.685260071427578E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 5.543452351255314E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11241.0 32-33 24393.0 34-35 26835.0 36-37 27835.0 38-39 29323.0 40-41 30189.0 42-43 30950.0 44-45 30215.0 46-47 29765.0 48-49 28949.0 50-51 27912.0 52-53 26642.0 54-55 24859.0 56-57 23467.0 58-59 22001.0 60-61 20665.0 62-63 18904.0 64-65 17587.0 66-67 15881.0 68-69 14530.0 70-71 13443.0 72-73 12214.0 74-75 11013.0 76-77 9846.0 78-79 8907.0 80-81 8119.0 82-83 7252.0 84-85 6510.0 86-87 5882.0 88-89 5410.0 90-91 4949.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83311604086215 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8412949024 99.67467579661236 2 0.15133874474992895 0.3021723693219615 3 0.0067852769439588866 0.02032186021546902 4 3.2587545967129484E-4 0.0013013265032093468 5 0.0 0.0 6 2.5520044643948284E-4 0.0015286473469843633 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 2 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 3 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 4 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 5 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 6 0.0015633468128569642 0.0 0.0 0.0 1.7370520142855156E-4 7 0.0019107572157140674 0.0 0.0 0.0 3.4741040285710313E-4 8 0.004690040438570893 0.0 0.0 0.0 3.4741040285710313E-4 9 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 3.4741040285710313E-4 10-11 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 4.342630035713789E-4 12-13 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 14-15 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 16-17 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 18-19 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 20-21 0.004690040438570893 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 22-23 0.004776893039285168 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 24-25 0.005037450841427996 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 26-27 0.005471713844999374 1.7370520142855156E-4 0.0 0.0 6.948208057142063E-4 28-29 0.006079682049999305 1.7370520142855156E-4 0.0 0.0 7.816734064284821E-4 30-31 0.006079682049999305 1.7370520142855156E-4 0.0 0.0 8.685260071427578E-4 32-33 0.006079682049999305 1.7370520142855156E-4 0.0 0.0 8.685260071427578E-4 34-35 0.00660079765428496 1.7370520142855156E-4 0.0 0.0 8.685260071427578E-4 36-37 0.007035060657856338 1.7370520142855156E-4 0.0 0.0 0.0010422312085713095 38-39 0.007121913258570614 1.7370520142855156E-4 0.0 0.0 0.0010422312085713095 40-41 0.007295618459999167 1.7370520142855156E-4 0.0 0.0 0.0010422312085713095 42-43 0.007295618459999167 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 44-45 0.007295618459999167 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 46-47 0.007295618459999167 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 48-49 0.00764302886285627 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 50-51 0.00764302886285627 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 52-53 0.007990439265713372 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 54-55 0.0082509970678562 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 56-57 0.008337849668570476 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 58-59 0.008337849668570476 1.7370520142855156E-4 0.0 1.7370520142855156E-4 0.0010422312085713095 60-61 0.008511554869999027 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1.7370520142855156E-4 0.0010422312085713095 >>END_MODULE