##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-15B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 607169 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.25127270990449 40.0 40.0 40.0 40.0 40.0 2 39.16891013869285 40.0 40.0 40.0 40.0 40.0 3 39.159957112434924 40.0 40.0 40.0 40.0 40.0 4 39.081553900149714 40.0 40.0 40.0 40.0 40.0 5 39.18914832608384 40.0 40.0 40.0 40.0 40.0 6 39.262096714423826 40.0 40.0 40.0 40.0 40.0 7 39.281669518700724 40.0 40.0 40.0 40.0 40.0 8 39.30819261194165 40.0 40.0 40.0 40.0 40.0 9 39.336395632846866 40.0 40.0 40.0 40.0 40.0 10-11 39.328971011365866 40.0 40.0 40.0 40.0 40.0 12-13 39.32695509816871 40.0 40.0 40.0 40.0 40.0 14-15 39.34103684476645 40.0 40.0 40.0 40.0 40.0 16-17 39.478794207214136 40.0 40.0 40.0 40.0 40.0 18-19 39.490003606903514 40.0 40.0 40.0 40.0 40.0 20-21 39.50310374870917 40.0 40.0 40.0 40.0 40.0 22-23 39.498409833176595 40.0 40.0 40.0 40.0 40.0 24-25 39.507804252193374 40.0 40.0 40.0 40.0 40.0 26-27 39.49350510319203 40.0 40.0 40.0 40.0 40.0 28-29 39.50007658493764 40.0 40.0 40.0 40.0 40.0 30-31 39.49355155602421 40.0 40.0 40.0 40.0 40.0 32-33 39.4894758718158 40.0 40.0 40.0 40.0 40.0 34-35 39.488632794285756 40.0 40.0 40.0 40.0 40.0 36-37 39.48550084353542 40.0 40.0 40.0 40.0 40.0 38-39 39.47519018963476 40.0 40.0 40.0 40.0 40.0 40-41 39.46507731822543 40.0 40.0 40.0 40.0 40.0 42-43 39.458279743740675 40.0 40.0 40.0 40.0 40.0 44-45 39.437309368194974 40.0 40.0 40.0 40.0 40.0 46-47 39.45007484067659 40.0 40.0 40.0 40.0 40.0 48-49 39.43791122625882 40.0 40.0 40.0 40.0 40.0 50-51 39.43804286290343 40.0 40.0 40.0 40.0 40.0 52-53 39.43088939917037 40.0 40.0 40.0 40.0 40.0 54-55 39.41368676236871 40.0 40.0 40.0 40.0 40.0 56-57 39.40919634168162 40.0 40.0 40.0 40.0 40.0 58-59 39.386631485934714 40.0 40.0 40.0 40.0 40.0 60-61 39.36344034748526 40.0 40.0 40.0 40.0 40.0 62-63 39.35302998574838 40.0 40.0 40.0 40.0 40.0 64-65 39.36228078012145 40.0 40.0 40.0 40.0 40.0 66-67 39.36278325913036 40.0 40.0 40.0 40.0 40.0 68-69 39.34671023253652 40.0 40.0 40.0 40.0 40.0 70-71 39.34634265127912 40.0 40.0 40.0 40.0 40.0 72-73 39.33091100325741 40.0 40.0 40.0 40.0 40.0 74-75 39.339198352387456 40.0 40.0 40.0 40.0 40.0 76-77 39.31752665960405 40.0 40.0 40.0 40.0 40.0 78-79 39.31718708108819 40.0 40.0 40.0 40.0 40.0 80-81 39.322737537014575 40.0 40.0 40.0 40.0 40.0 82-83 39.314121123454136 40.0 40.0 40.0 40.0 40.0 84-85 39.32536652608741 40.0 40.0 40.0 40.0 40.0 86-87 39.349084757899156 40.0 40.0 40.0 40.0 40.0 88-89 39.39054429430821 40.0 40.0 40.0 40.0 40.0 90-91 39.46365007541478 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17827178643632635 1101 2 0.14495985186931648 1101 3 0.1125983215425066 1101 4 -0.09056484935178588 1101 5 0.04587914194947729 1101 6 0.12399584990134827 1101 7 0.04829310745080306 1101 8 0.007861646723576143 1101 9 0.08359100368458883 1101 10-11 -0.01286010096804091 1101 12-13 0.09094118393404216 1101 14-15 0.05697570250313788 1101 16-17 0.11613436584342907 1101 18-19 0.03892161535631544 1101 20-21 0.051632067414637106 1101 22-23 0.03941235812200006 1101 24-25 0.03146193249938278 1101 26-27 0.026332716135392786 1101 28-29 0.04655153647851051 1101 30-31 0.05601263254203559 1101 32-33 0.031787446432332445 1101 34-35 0.023330080794920605 1101 36-37 0.07612187406855497 1101 38-39 0.21402877688438338 1101 40-41 0.06686975987030763 1101 42-43 0.009244901730177446 1101 44-45 0.051251047914568915 1101 46-47 0.09041021704246077 1101 48-49 -0.013728056549794587 1101 50-51 0.10567503320736193 1101 52-53 0.045055757110077366 1101 54-55 0.09540627913550281 1101 56-57 0.07455293760202863 1101 58-59 -0.023952193507852826 1101 60-61 0.10409946867569175 1101 62-63 -0.059680527714576215 1101 64-65 0.13123018698109945 1101 66-67 -0.011746732286880501 1101 68-69 0.15316387564649858 1101 70-71 0.04941779229488219 1101 72-73 0.06010152569164262 1101 74-75 -0.10877204101612392 1101 76-77 -0.13882397215991915 1101 78-79 0.08969126247892234 1101 80-81 0.24176511386008315 1101 82-83 0.3327408128821219 1101 84-85 -0.05555928845912206 1101 86-87 -0.24559810400842252 1101 88-89 0.012826196032925452 1101 90-91 0.10899969404041343 1102 1 0.3511994183847449 1102 2 0.0013119090179500859 1102 3 0.07336883967663965 1102 4 -0.27471643700103243 1102 5 -0.1252774214947152 1102 6 -0.09552319402716591 1102 7 0.08224622490427436 1102 8 -0.04066437230350317 1102 9 0.07811156393888297 1102 10-11 -0.13659677695729044 1102 12-13 -0.08686834308463887 1102 14-15 -0.04726574148828888 1102 16-17 -0.05522707006091565 1102 18-19 -0.0695781206906787 1102 20-21 -7.011648303105744E-4 1102 22-23 -0.20612099542901063 1102 24-25 -0.021555106509502764 1102 26-27 -0.19855614280137956 1102 28-29 0.01699419128414803 1102 30-31 -0.017251736048685018 1102 32-33 -0.06969703165507468 1102 34-35 -0.1003186645492562 1102 36-37 -0.12529494832207178 1102 38-39 -0.05805433419381245 1102 40-41 -0.013447455274047115 1102 42-43 -0.042135102550595604 1102 44-45 -0.11234673945097029 1102 46-47 -0.10564412572601611 1102 48-49 -0.09909183419568279 1102 50-51 -0.120923703796052 1102 52-53 -0.02175931021285038 1102 54-55 -0.11934321855641628 1102 56-57 -0.07059179865724019 1102 58-59 -0.11071113293282053 1102 60-61 -0.19686681972121534 1102 62-63 -0.15344429882316035 1102 64-65 -0.23686601177723787 1102 66-67 -0.15289502300495172 1102 68-69 -0.4728943033726267 1102 70-71 -0.36980920709098086 1102 72-73 -0.8020329618505428 1102 74-75 -0.6712505042544734 1102 76-77 -0.41024382267496406 1102 78-79 -0.30381712831450614 1102 80-81 0.15156126088353972 1102 82-83 0.3598075344133065 1102 84-85 0.2346493792776343 1102 86-87 0.4380382596279446 1102 88-89 0.6386882649984429 1102 90-91 -2.9132225281818123 1103 1 0.32056661546997134 1103 2 0.22058529148087302 1103 3 -0.07892755597887913 1103 4 0.31844747111652794 1103 5 0.012663340892139274 1103 6 0.139787831386613 1103 7 0.15872443728993346 1103 8 0.0190576170455401 1103 9 0.03206921029634913 1103 10-11 0.0012029308201348954 1103 12-13 -0.05832208382626902 1103 14-15 -0.008909533355549115 1103 16-17 0.0769756972708322 1103 18-19 -0.10626296477210673 1103 20-21 0.015754319133371553 1103 22-23 -0.06188866518024838 1103 24-25 0.00531712728984246 1103 26-27 -0.0156349284542614 1103 28-29 0.00947305305329138 1103 30-31 -0.010499744650346088 1103 32-33 -0.022734256076901715 1103 34-35 -0.030140780519992916 1103 36-37 0.055584203948242816 1103 38-39 0.05001582296606699 1103 40-41 0.020196446401840262 1103 42-43 -0.04996033833012348 1103 44-45 0.09194697480857883 1103 46-47 0.016641044108155256 1103 48-49 0.026990997833905794 1103 50-51 -0.08452574017282188 1103 52-53 0.14126963714248575 1103 54-55 0.009601435347470044 1103 56-57 5.754963791488876E-4 1103 58-59 -0.1349004028471228 1103 60-61 -0.119576241417235 1103 62-63 -0.11611431526129934 1103 64-65 0.1192253374225487 1103 66-67 -0.1117391164795265 1103 68-69 -0.20609630949957136 1103 70-71 -0.27832462975002414 1103 72-73 -0.05200644076538197 1103 74-75 0.18154850614158136 1103 76-77 -0.17636806345812062 1103 78-79 0.07829135146765509 1103 80-81 -0.31841829990541726 1103 82-83 0.25816227860983076 1103 84-85 -0.7628142767693831 1103 86-87 0.25761272771305244 1103 88-89 0.21763563341949776 1103 90-91 0.6423330273737449 1104 1 0.475481898622526 1104 2 0.2899881739252521 1104 3 0.22570493149159176 1104 4 0.05196672367944899 1104 5 0.14969347268937838 1104 6 0.19060227250609785 1104 7 0.0944917806120742 1104 8 0.009291635042103508 1104 9 0.008533367749741672 1104 10-11 0.02689216369441283 1104 12-13 -0.016681747013045367 1104 14-15 0.044202785882383466 1104 16-17 -0.040654772593022415 1104 18-19 -0.007791924129264771 1104 20-21 0.03852942475743504 1104 22-23 0.06067527937126016 1104 24-25 -0.01428446938728456 1104 26-27 0.030778727905023118 1104 28-29 0.11321702765128805 1104 30-31 0.13703795708855893 1104 32-33 0.026686300700518473 1104 34-35 0.006792523382323168 1104 36-37 -0.04857858531129722 1104 38-39 -0.04897185623932643 1104 40-41 0.051318755502023805 1104 42-43 0.05589800840382253 1104 44-45 -0.07487443239173075 1104 46-47 0.04820253425612009 1104 48-49 -0.03662495210520689 1104 50-51 -0.057545676373194965 1104 52-53 0.05211769126523791 1104 54-55 -0.039985682505800924 1104 56-57 -0.010970042280199266 1104 58-59 0.07276260673394574 1104 60-61 -0.1145744862999507 1104 62-63 -0.18810101088020303 1104 64-65 -0.0947192271097208 1104 66-67 -0.271377605480545 1104 68-69 -0.12852793172884702 1104 70-71 -0.22720804703342878 1104 72-73 -0.25637623092468687 1104 74-75 -0.014264187064441103 1104 76-77 -0.2933103792339793 1104 78-79 -0.5670742575271959 1104 80-81 -0.2219135665239591 1104 82-83 -0.7650103858224782 1104 84-85 -0.24584205780352875 1104 86-87 0.08291630840842856 1104 88-89 -0.9327403064301265 1104 90-91 -0.024333639292926534 1201 1 0.32953503663334516 1201 2 0.14203665028873047 1201 3 0.14529875331855635 1201 4 0.07719957185740611 1201 5 0.11617627935194008 1201 6 0.23645741739272097 1201 7 0.19206276769077846 1201 8 -0.008620876890908846 1201 9 0.009051139322210133 1201 10-11 0.06415809134435335 1201 12-13 0.022903933537229193 1201 14-15 -0.015284739484584975 1201 16-17 0.006453924521679255 1201 18-19 0.02902606298265198 1201 20-21 0.028701984056333174 1201 22-23 -0.010560891742024126 1201 24-25 -0.06908394786868399 1201 26-27 0.03514316755781266 1201 28-29 -0.01792139704573259 1201 30-31 -0.021511703421914774 1201 32-33 -0.056006333200599556 1201 34-35 -0.05663774518497888 1201 36-37 0.019706832317517353 1201 38-39 -0.07876131103609652 1201 40-41 -0.0669606574358852 1201 42-43 -0.1088988911700639 1201 44-45 -0.01498734960861725 1201 46-47 -0.12609490234828513 1201 48-49 -0.043740697514159876 1201 50-51 -0.11677427251483152 1201 52-53 -0.0517376977237376 1201 54-55 0.14204164290411114 1201 56-57 -4.7989913236534676E-4 1201 58-59 -0.29452356658832457 1201 60-61 -0.10465594098222653 1201 62-63 -0.24861858024412697 1201 64-65 -0.08331694943591827 1201 66-67 -0.10733706972033019 1201 68-69 0.04668422708385833 1201 70-71 0.1305792827270622 1201 72-73 0.03400570260473046 1201 74-75 0.3883502929073046 1201 76-77 0.13392662786235832 1201 78-79 -0.15940891119427647 1201 80-81 -0.3444508188480526 1201 82-83 -0.5316068451275981 1201 84-85 -0.043672299044047236 1201 86-87 -0.008196358911845891 1201 88-89 0.41646604277622146 1201 90-91 0.6423330273737449 1202 1 0.04529443530388022 1202 2 0.1684255357279838 1202 3 0.09114168008886026 1202 4 0.15176303425403148 1202 5 0.08441092964580577 1202 6 0.13239542992234732 1202 7 0.07428723631579004 1202 8 0.040524013605228504 1202 9 0.009062730098271743 1202 10-11 0.02081107261614079 1202 12-13 -0.04815414701369747 1202 14-15 0.014714672697479614 1202 16-17 0.021456528306735834 1202 18-19 0.06876669453092177 1202 20-21 0.04739542210404579 1202 22-23 0.03735703231686216 1202 24-25 -0.011303929207535646 1202 26-27 0.020249020320186162 1202 28-29 0.01649989620338488 1202 30-31 -0.011873973127677573 1202 32-33 0.04114268431322898 1202 34-35 -0.03806679411932379 1202 36-37 0.012482064094946566 1202 38-39 0.02441856471941861 1202 40-41 -0.010022299956084169 1202 42-43 0.003469040605779128 1202 44-45 -0.011839932065306868 1202 46-47 0.09464664273294687 1202 48-49 -0.033766598045772866 1202 50-51 -0.007116769371307896 1202 52-53 -0.02238727326974299 1202 54-55 0.05927282370762299 1202 56-57 0.008718901589510608 1202 58-59 -0.035644793573673894 1202 60-61 -0.0055880258817282424 1202 62-63 -0.09039946147485978 1202 64-65 -0.011758297039605736 1202 66-67 0.020963559762179784 1202 68-69 -0.03552576146049091 1202 70-71 -0.03141442191727606 1202 72-73 -0.11790134307879896 1202 74-75 0.07485715868713783 1202 76-77 0.21850898118088224 1202 78-79 -0.4682941639985927 1202 80-81 -0.21907768451969645 1202 82-83 -0.09010193444559178 1202 84-85 0.3561683666194 1202 86-87 -0.6325873239388216 1202 88-89 0.03868826499844147 1202 90-91 0.002333027373744301 1203 1 0.29782407297294355 1203 2 0.14155722954760108 1203 3 0.16536028721780127 1203 4 0.16062372329505337 1203 5 -0.002542135099879772 1203 6 0.07756156784186885 1203 7 0.027280422236003687 1203 8 0.023604104883688137 1203 9 0.017389106232293727 1203 10-11 0.1171686806117549 1203 12-13 0.07495568132237196 1203 14-15 -0.10402205892954441 1203 16-17 0.05033327853930558 1203 18-19 -0.01450978854519036 1203 20-21 0.08863451917883935 1203 22-23 0.0019238174546885034 1203 24-25 0.012662230975024613 1203 26-27 -0.011219783598107824 1203 28-29 0.0918857225993932 1203 30-31 0.028488914388326236 1203 32-33 0.041186957810531055 1203 34-35 -0.0038508656189790713 1203 36-37 -0.009728626451753541 1203 38-39 0.007323872734993131 1203 40-41 0.03116290146474654 1203 42-43 0.058742253051654814 1203 44-45 -0.003927652924147651 1203 46-47 0.11109318380940181 1203 48-49 0.12956189916524607 1203 50-51 0.12129144571439099 1203 52-53 0.11564189918489376 1203 54-55 -0.06459541747554454 1203 56-57 0.05529753554755956 1203 58-59 0.10125714395547902 1203 60-61 0.06584272942094316 1203 62-63 -0.08652451038993547 1203 64-65 0.0547389589109315 1203 66-67 0.20267544230112122 1203 68-69 0.058098932354326394 1203 70-71 -0.023730722248444636 1203 72-73 0.10375203930802002 1203 74-75 0.25483585722939495 1203 76-77 0.0972684863448805 1203 78-79 0.028781412095383985 1203 80-81 0.35617542058415097 1203 82-83 -0.02867157718954161 1203 84-85 0.08977851980003493 1203 86-87 0.27865841466670105 1203 88-89 -0.27503722519763585 1203 90-91 0.1717447920796289 1204 1 0.3081355860196595 1204 2 0.13656462987624707 1204 3 0.03912505388408505 1204 4 -0.2730922517030976 1204 5 0.016819144671387676 1204 6 -0.01691557271775679 1204 7 -0.2096388656231838 1204 8 -0.036328845419511424 1204 9 -0.14397612815029248 1204 10-11 -0.2373175501153426 1204 12-13 -0.10709861883184146 1204 14-15 -0.09517429592652604 1204 16-17 -0.06742401398928166 1204 18-19 -0.021038393437564196 1204 20-21 -0.0807626939467454 1204 22-23 -0.16960220412085647 1204 24-25 -0.006985738693337851 1204 26-27 -0.05860441810733619 1204 28-29 -0.1404682811532112 1204 30-31 -0.18771178407839528 1204 32-33 -0.013631344256033628 1204 34-35 -0.06210319898128347 1204 36-37 -0.05579473703344462 1204 38-39 -0.0897980243151082 1204 40-41 0.013788596092737748 1204 42-43 -0.14235067324985806 1204 44-45 -0.0968661482382629 1204 46-47 -0.08910972443432286 1204 48-49 0.020904476608031075 1204 50-51 -0.22357128455894326 1204 52-53 0.03413234096923645 1204 54-55 -0.08514149634162749 1204 56-57 -0.15574097562282674 1204 58-59 -0.022764417420368943 1204 60-61 -0.08624173323674 1204 62-63 -0.18847503834339108 1204 64-65 -0.31892314620672835 1204 66-67 -0.1997706524577083 1204 68-69 -0.21669763883086546 1204 70-71 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0.12519389271269432 2401 50-51 0.0740000273196415 2401 52-53 0.03968933269613473 2401 54-55 0.19692769376310792 2401 56-57 0.16086718784607257 2401 58-59 0.21184351092897202 2401 60-61 0.18827126237927416 2401 62-63 0.0673869740174382 2401 64-65 0.18758069081367523 2401 66-67 0.05052194492705553 2401 68-69 0.18706143105670492 2401 70-71 0.15791195053710538 2401 72-73 0.2710544189310298 2401 74-75 0.18475959900784744 2401 76-77 -0.015010768791519524 2401 78-79 0.27561355464814596 2401 80-81 0.2809555373745809 2401 82-83 0.27699726519887236 2401 84-85 0.2023222127732538 2401 86-87 -0.04076771052130823 2401 88-89 -0.32131173500155796 2401 90-91 -0.2769216310113549 2402 1 0.30084917822789237 2402 2 0.20520918651002518 2402 3 0.13465183043730633 2402 4 0.22687456793354954 2402 5 0.08182815441620761 2402 6 0.16061162241850724 2402 7 0.1796168222567971 2402 8 0.09091529569401757 2402 9 0.06062420161147486 2402 10-11 0.11651376091738541 2402 12-13 0.1112465488310832 2402 14-15 0.1426487659574036 2402 16-17 0.03137179405465673 2402 18-19 0.02169791455788328 2402 20-21 0.025897370678400478 2402 22-23 0.052030371849419055 2402 24-25 -0.031913890445004256 2402 26-27 0.01582424155912321 2402 28-29 -0.021228264614073566 2402 30-31 0.043307771917305615 2402 32-33 0.07050970361923703 2402 34-35 0.03735233331683929 2402 36-37 -0.004925799643068274 2402 38-39 0.029738120372520882 2402 40-41 0.10251445357616262 2402 42-43 0.04039229984285697 2402 44-45 0.004313996737678849 2402 46-47 0.0491532337925662 2402 48-49 0.050542352055948925 2402 50-51 0.08534037574310815 2402 52-53 0.08193616234029122 2402 54-55 -7.174493666539661E-4 2402 56-57 0.009178632356430683 2402 58-59 0.15752325961180702 2402 60-61 0.0660678165373696 2402 62-63 0.1631233984087075 2402 64-65 0.15225316356789165 2402 66-67 0.09777281919607361 2402 68-69 -0.1320309367055046 2402 70-71 0.003803412670698947 2402 72-73 0.16052378457073502 2402 74-75 0.12880749488856225 2402 76-77 0.13315143080841807 2402 78-79 0.23308753058914533 2402 80-81 -0.04272590730421655 2402 82-83 -0.01246420791825642 2402 84-85 -0.037667004903156 2402 86-87 -0.2821926206029417 2402 88-89 -0.43929655211680796 2402 90-91 0.6423330273737449 2403 1 -0.47750130246025435 2403 2 -0.4279768482344508 2403 3 -0.05586933670723937 2403 4 -0.03737910047596671 2403 5 0.033509719604268184 2403 6 0.05987962714105777 2403 7 0.07535640734703009 2403 8 0.13565133887161807 2403 9 0.1496442724497271 2403 10-11 0.10453872296832145 2403 12-13 0.07645484702639749 2403 14-15 0.09354436386598763 2403 16-17 0.02009839213190645 2403 18-19 0.08654527498966047 2403 20-21 0.08631902694421001 2403 22-23 0.09827088458343525 2403 24-25 0.10605203972871635 2403 26-27 0.05296709870197702 2403 28-29 0.06433085159439145 2403 30-31 0.14045400231028538 2403 32-33 -0.007466616162773221 2403 34-35 0.11211799039207904 2403 36-37 0.09804205758864981 2403 38-39 0.046579986506706916 2403 40-41 0.08422289473959665 2403 42-43 0.14225961487318983 2403 44-45 0.14739546786002933 2403 46-47 0.04254537092508315 2403 48-49 0.14224942364966608 2403 50-51 0.14744284249794504 2403 52-53 -0.004020940666357831 2403 54-55 0.09414914270869446 2403 56-57 0.051558865386766684 2403 58-59 0.09460505071082537 2403 60-61 0.09366896740254305 2403 62-63 0.17755327862552406 2403 64-65 0.17252369269038326 2403 66-67 0.009849205814795425 2403 68-69 0.1722573464152859 2403 70-71 0.11903401744873321 2403 72-73 0.23042025706274671 2403 74-75 0.2650777788403502 2403 76-77 0.023867812944210698 2403 78-79 0.045264928578902186 2403 80-81 0.07578108153518315 2403 82-83 -0.06089477649446451 2403 84-85 0.3913458078490635 2403 86-87 0.30715186761795366 2403 88-89 0.4684754990409985 2403 90-91 0.4137615988023171 2404 1 -0.36079635327409676 2404 2 -0.24209566175176178 2404 3 -0.4388818129527934 2404 4 -0.3989159095315671 2404 5 -0.16593174119057608 2404 6 -0.20186704723719373 2404 7 -0.04240613086663103 2404 8 -0.060769383263384213 2404 9 0.014537274184831972 2404 10-11 0.02897068198674191 2404 12-13 0.03753557081388692 2404 14-15 -0.0014172159076224489 2404 16-17 -0.005078836654341501 2404 18-19 -0.04842621968108318 2404 20-21 -0.03345954035315657 2404 22-23 -0.05560170898841221 2404 24-25 0.05257769452504846 2404 26-27 -0.020208147104845864 2404 28-29 0.021700098888452146 2404 30-31 0.08514870610273562 2404 32-33 -0.02087703985233702 2404 34-35 0.0919582419633116 2404 36-37 0.011605975218458298 2404 38-39 0.0035939972621648053 2404 40-41 0.07715090005274305 2404 42-43 -0.006027959359421686 2404 44-45 0.031385235960115665 2404 46-47 -0.004736272227361837 2404 48-49 0.06303162801480511 2404 50-51 0.05330815498595598 2404 52-53 0.0032043303370912213 2404 54-55 -0.08827600017582427 2404 56-57 0.025697371409926006 2404 58-59 0.017633996520999062 2404 60-61 0.06272257037328899 2404 62-63 0.18093916139187627 2404 64-65 -0.05370331687715435 2404 66-67 0.010096839191064078 2404 68-69 0.08587756486143405 2404 70-71 0.04362252828416047 2404 72-73 -0.16235192161535394 2404 74-75 -0.017029320899922595 2404 76-77 -0.07021277791057656 2404 78-79 0.2359116553184748 2404 80-81 -0.07812221306181755 2404 82-83 -0.06819274073740189 2404 84-85 -9.126645179833304E-4 2404 86-87 0.3264333213563333 2404 88-89 0.1711557974659783 2404 90-91 0.36647095840822885 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 321.0 32 2289.0 33 4227.0 34 5909.0 35 7112.0 36 10340.0 37 15863.0 38 41873.0 39 255870.0 40 263353.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.503446401660202 12.451021567803938 32.35697638988397 28.68855564065189 2 26.129960485766475 18.790096899639778 30.377699411816057 24.70224320277769 3 26.151540355587034 19.812571541508486 22.89229451631764 31.143593586586842 4 26.092570602254067 21.691818916973695 21.500933018648844 30.714677462123397 5 26.216424092797887 22.04542722042792 21.46947554964104 30.268673137133156 6 26.82613901566121 24.454476430779568 23.942592589542617 24.77679196401661 7 25.114259785990388 24.25469679776141 23.867984037393214 26.76305937885498 8 24.241356195721455 25.31535700933348 24.355327758828267 26.087959036116796 9 23.96235644441663 25.489937727387268 24.826036902411026 25.721668925785078 10-11 23.8217036772299 24.242509087255772 24.77465087973859 27.16113635577574 12-13 24.17975884803078 23.95000403512037 24.52504986255886 27.345187254289993 14-15 24.680113773924557 23.670592536839003 24.32354089223923 27.32575279699721 16-17 24.65178558193847 23.41580350775484 24.164030113526877 27.768380796779805 18-19 24.806355396932318 23.75755349828466 23.944157228053477 27.49193387672954 20-21 24.92460912859517 23.873419097483566 23.808363075189938 27.393608698731324 22-23 24.642974196640473 23.486212240743516 24.08011607970763 27.79069748290838 24-25 24.703830399773373 23.842455724847614 24.092468489003885 27.361245386375128 26-27 24.95639599518421 23.833973737130847 24.01497770801869 27.194652559666256 28-29 24.97121888633972 23.54640964871395 24.05681120083535 27.42556026411098 30-31 25.05404210219454 23.78041901260859 24.08840752644872 27.07713135874815 32-33 25.0605438111286 23.789987785870892 24.155147256541387 26.99432114645913 34-35 25.02448101128832 23.43098973106671 24.32827770630816 27.21625155133681 36-37 25.056572385411407 23.7306173745328 24.27627301488135 26.93653722517444 38-39 25.081205417680053 23.810956254881102 24.328981817388158 26.77885651005068 40-41 25.073916981553673 23.511485561749108 24.424425123394474 26.99017233330274 42-43 25.064870022721642 23.789822180750395 24.52165936516472 26.623648431363243 44-45 25.104973563457776 23.748659137682765 24.496518796706237 26.64984850215322 46-47 25.19814862162156 23.501011949849342 24.5454588899854 26.7553805385437 48-49 25.265304082498325 23.78964823901652 24.416177237608146 26.52887044087701 50-51 25.351205337270482 23.83247208190598 24.372447695910505 26.443874884913033 52-53 25.22587169019887 23.66293397251039 24.55728249878282 26.553911838507922 54-55 25.455070411736973 23.77311646768466 24.486287124594437 26.285525995983928 56-57 25.543887309474467 23.782473612681606 24.308079745169312 26.365559332674614 58-59 25.536556037027808 23.69360056024277 24.343490022146852 26.42635338058257 60-61 25.616212463114042 23.881270612740842 24.310449574726608 26.1920673494185 62-63 25.77821387830862 23.805127511069006 24.09877032539974 26.317888285222637 64-65 25.755798677606258 23.60047565002343 24.2570677097018 26.386657962668515 66-67 25.88020468524902 23.781660911295923 24.254529104639673 26.08360529881538 68-69 25.994216229399676 23.719382599909412 23.98557541549075 26.30082575520017 70-71 25.84101589841016 23.446455354464554 24.162783721627836 26.54974502549745 72-73 26.11537408144981 23.746060040571717 24.18566435027226 25.952901527706214 74-75 26.220223494140093 23.64186426819297 23.854456255110385 26.283455982556553 76-77 26.542525965232826 23.16299622264985 23.83877258797079 26.45570522414653 78-79 26.832565670539037 23.20516246679138 23.848898809991667 26.113373052677915 80-81 26.8100882969901 23.29967315478804 23.27820869310698 26.612029855114884 82-83 26.924854191904686 22.895929862247254 23.233130596595522 26.94608534925254 84-85 27.813738772642882 22.443006004649842 22.541689664977334 27.20156555772994 86-87 28.319030792529027 21.581211990096392 22.304752289608423 27.795004927766158 88-89 27.801281276441436 20.762319190942424 22.587533744308796 28.848865788307343 90-91 24.894390411631793 21.809608016504566 25.346301208370175 27.94970036349347 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 3.5 10 4.5 11 5.0 12 11.5 13 17.5 14 28.0 15 47.5 16 63.0 17 75.0 18 97.0 19 139.5 20 201.0 21 294.5 22 430.5 23 630.0 24 890.5 25 1139.0 26 1407.5 27 1782.0 28 2302.0 29 2912.5 30 3632.0 31 4494.5 32 5456.5 33 6479.5 34 7833.0 35 9460.5 36 11261.5 37 13199.0 38 15351.0 39 18086.0 40 20862.5 41 23745.0 42 26800.0 43 29688.0 44 32631.5 45 35437.0 46 38155.0 47 40793.0 48 42748.0 49 44253.0 50 45582.0 51 46272.0 52 46282.5 53 45909.0 54 45118.0 55 43840.5 56 42421.0 57 40768.0 58 38789.5 59 36700.0 60 34520.5 61 32252.0 62 30218.0 63 28084.0 64 25447.5 65 22860.0 66 20369.5 67 18196.5 68 16079.0 69 13741.0 70 11610.5 71 9747.5 72 8077.0 73 6415.5 74 4922.0 75 3855.0 76 2981.5 77 2179.5 78 1616.0 79 1182.0 80 816.5 81 562.0 82 396.5 83 265.5 84 158.0 85 103.5 86 70.0 87 48.5 88 32.5 89 22.0 90 17.5 91 13.5 92 12.0 93 8.0 94 4.5 95 3.5 96 2.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002305783068634927 2 0.007576144368371903 3 6.58795162467122E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9171.0 32-33 19692.0 34-35 21975.0 36-37 23257.0 38-39 25267.0 40-41 26784.0 42-43 27450.0 44-45 27968.0 46-47 28330.0 48-49 28503.0 50-51 28142.0 52-53 27527.0 54-55 26286.0 56-57 25711.0 58-59 24719.0 60-61 23728.0 62-63 22629.0 64-65 21359.0 66-67 20339.0 68-69 18823.0 70-71 17702.0 72-73 16261.0 74-75 14872.0 76-77 13802.0 78-79 12664.0 80-81 11529.0 82-83 10407.0 84-85 9316.0 86-87 8549.0 88-89 7543.0 90-91 6864.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.8264651412304 #Duplication Level Percentage of deduplicated Percentage of total 1 99.83450975260602 99.66126207710352 2 0.15769998880192926 0.3148526486981643 3 0.007512248715562713 0.02249763703609122 4 0.0 0.0 5 2.7800987649043775E-4 0.0013876371622195227 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0029645782311020492 0.0 0.0 0.0 0.0 2 0.0029645782311020492 0.0 0.0 0.0 0.0 3 0.0029645782311020492 1.646987906167805E-4 0.0 0.0 1.646987906167805E-4 4 0.0031292770217188296 1.646987906167805E-4 0.0 0.0 1.646987906167805E-4 5 0.0031292770217188296 1.646987906167805E-4 0.0 0.0 1.646987906167805E-4 6 0.0031292770217188296 1.646987906167805E-4 0.0 0.0 3.29397581233561E-4 7 0.0036233733935691712 1.646987906167805E-4 0.0 0.0 3.29397581233561E-4 8 0.009058433483922927 1.646987906167805E-4 0.0 0.0 3.29397581233561E-4 9 0.009223132274539709 1.646987906167805E-4 0.0 0.0 3.29397581233561E-4 10-11 0.009223132274539709 1.646987906167805E-4 0.0 0.0 4.940963718503415E-4 12-13 0.009387831065156489 1.646987906167805E-4 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1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 36-37 0.012352409296258537 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 38-39 0.012599457482183708 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 40-41 0.012681806877492098 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 42-43 0.01301120445872566 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 44-45 0.01325825264465083 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 46-47 0.013752349016501172 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 48-49 0.013917047807117952 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 50-51 0.014081746597734732 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 52-53 0.014328794783659903 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 54-55 0.014328794783659903 1.646987906167805E-4 3.29397581233561E-4 0.0 4.940963718503415E-4 56-57 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