##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-15A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 555399 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.2676508240022 40.0 40.0 40.0 40.0 40.0 2 39.16154692392316 40.0 40.0 40.0 40.0 40.0 3 39.16287209735704 40.0 40.0 40.0 40.0 40.0 4 39.060884157155485 40.0 40.0 40.0 40.0 40.0 5 39.19323585386362 40.0 40.0 40.0 40.0 40.0 6 39.26397418792616 40.0 40.0 40.0 40.0 40.0 7 39.294424368787126 40.0 40.0 40.0 40.0 40.0 8 39.31515180978 40.0 40.0 40.0 40.0 40.0 9 39.338039859632445 40.0 40.0 40.0 40.0 40.0 10-11 39.32736645186614 40.0 40.0 40.0 40.0 40.0 12-13 39.32412553857677 40.0 40.0 40.0 40.0 40.0 14-15 39.342400688514026 40.0 40.0 40.0 40.0 40.0 16-17 39.48002787185429 40.0 40.0 40.0 40.0 40.0 18-19 39.49316797473528 40.0 40.0 40.0 40.0 40.0 20-21 39.49849027455937 40.0 40.0 40.0 40.0 40.0 22-23 39.503690139881414 40.0 40.0 40.0 40.0 40.0 24-25 39.50897642955785 40.0 40.0 40.0 40.0 40.0 26-27 39.497355954908095 40.0 40.0 40.0 40.0 40.0 28-29 39.4964773073052 40.0 40.0 40.0 40.0 40.0 30-31 39.48538963661286 40.0 40.0 40.0 40.0 40.0 32-33 39.49666770002275 40.0 40.0 40.0 40.0 40.0 34-35 39.48979535728462 40.0 40.0 40.0 40.0 40.0 36-37 39.48304435464546 40.0 40.0 40.0 40.0 40.0 38-39 39.47521343476333 40.0 40.0 40.0 40.0 40.0 40-41 39.45976276107679 40.0 40.0 40.0 40.0 40.0 42-43 39.4661058235511 40.0 40.0 40.0 40.0 40.0 44-45 39.441331664025306 40.0 40.0 40.0 40.0 40.0 46-47 39.44227349351053 40.0 40.0 40.0 40.0 40.0 48-49 39.440580675452885 40.0 40.0 40.0 40.0 40.0 50-51 39.43928318504124 40.0 40.0 40.0 40.0 40.0 52-53 39.44294988887401 40.0 40.0 40.0 40.0 40.0 54-55 39.40779126270142 40.0 40.0 40.0 40.0 40.0 56-57 39.41257160743406 40.0 40.0 40.0 40.0 40.0 58-59 39.39992004913215 40.0 40.0 40.0 40.0 40.0 60-61 39.379185836421726 40.0 40.0 40.0 40.0 40.0 62-63 39.356295669344846 40.0 40.0 40.0 40.0 40.0 64-65 39.350873720850856 40.0 40.0 40.0 40.0 40.0 66-67 39.34442824226928 40.0 40.0 40.0 40.0 40.0 68-69 39.35552812070711 40.0 40.0 40.0 40.0 40.0 70-71 39.342712297814295 40.0 40.0 40.0 40.0 40.0 72-73 39.351154600256564 40.0 40.0 40.0 40.0 40.0 74-75 39.34585516244353 40.0 40.0 40.0 40.0 40.0 76-77 39.34539575053395 40.0 40.0 40.0 40.0 40.0 78-79 39.31930684721871 40.0 40.0 40.0 40.0 40.0 80-81 39.31945785086089 40.0 40.0 40.0 40.0 40.0 82-83 39.31653779226465 40.0 40.0 40.0 40.0 40.0 84-85 39.328155097488434 40.0 40.0 40.0 40.0 40.0 86-87 39.353843722326 40.0 40.0 40.0 40.0 40.0 88-89 39.36217008797654 40.0 40.0 40.0 40.0 40.0 90-91 39.387198024335646 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22216304319027103 1101 2 0.24323919614069212 1101 3 0.16455827584549354 1101 4 0.122861193183752 1101 5 -0.011306559010058947 1101 6 0.012699270632545279 1101 7 0.19824325019305888 1101 8 -0.016362317331804377 1101 9 0.004704017326297105 1101 10-11 -0.03555139024255283 1101 12-13 -6.437874134945787E-5 1101 14-15 -0.08611383648977977 1101 16-17 -0.0019945786128445775 1101 18-19 -0.007468536718000962 1101 20-21 -0.1068851236724555 1101 22-23 0.014575539881214183 1101 24-25 0.11196864055076361 1101 26-27 0.018850834276506134 1101 28-29 0.016815011425251214 1101 30-31 -0.10655358147619864 1101 32-33 -0.05808126269679548 1101 34-35 0.07208104543517635 1101 36-37 0.013379877599085432 1101 38-39 0.08026886920414 1101 40-41 0.04823216698815003 1101 42-43 0.03005730100015569 1101 44-45 -0.07403580341131288 1101 46-47 0.05570285176515455 1101 48-49 0.06220039994838089 1101 50-51 -0.015836705419623343 1101 52-53 0.11193685053556379 1101 54-55 0.06444350369909557 1101 56-57 -0.030659048365897945 1101 58-59 0.2091735499828289 1101 60-61 0.009904250330571074 1101 62-63 -0.05869967066416848 1101 64-65 -0.04404906980497714 1101 66-67 -0.005522158085170759 1101 68-69 -0.1279838911312936 1101 70-71 -0.0030075626288450508 1101 72-73 0.060497832010540264 1101 74-75 0.06875394592421458 1101 76-77 0.48426039925590203 1101 78-79 0.27702008388865806 1101 80-81 0.1842086238845866 1101 82-83 0.317953631320087 1101 84-85 0.144908655052582 1101 86-87 0.4624228587301644 1101 88-89 0.028012147865872805 1101 90-91 0.8308446251460921 1102 1 0.2421991363682281 1102 2 0.08737763787773645 1102 3 0.04853457697157637 1102 4 0.19153946965203517 1102 5 -0.1016680424817622 1102 6 0.0023388336696257284 1102 7 -0.009363617407082359 1102 8 0.0331648483868463 1102 9 0.11967438235880223 1102 10-11 -0.07906340547152979 1102 12-13 0.008522680836286156 1102 14-15 -0.12028917334901479 1102 16-17 0.056108271587952174 1102 18-19 0.03855168706424905 1102 20-21 0.09019181804212195 1102 22-23 0.08736363834631078 1102 24-25 -0.024910158375654134 1102 26-27 0.023978727318755944 1102 28-29 0.05357818250611501 1102 30-31 0.03962208298579384 1102 32-33 0.010560403785625283 1102 34-35 -0.09772430659764098 1102 36-37 0.04505440258460425 1102 38-39 -0.12574730665356526 1102 40-41 0.01779396839862102 1102 42-43 0.04567370645874291 1102 44-45 0.018993121721386785 1102 46-47 -0.11008200682645963 1102 48-49 -0.06950197948272319 1102 50-51 -0.05698625021629056 1102 52-53 -0.06689659920399293 1102 54-55 -0.10267915395008487 1102 56-57 -0.13277652791254724 1102 58-59 -0.04517561861772634 1102 60-61 -0.297675247641358 1102 62-63 -0.23737824513014516 1102 64-65 -0.29227979641623847 1102 66-67 -0.20199609592224022 1102 68-69 -0.3217539138900847 1102 70-71 -0.1763890206951828 1102 72-73 0.030345221337896078 1102 74-75 -0.2658080486602685 1102 76-77 -0.24946356082449483 1102 78-79 -0.24719077567626613 1102 80-81 -0.3158157726497137 1102 82-83 -0.11706768076294338 1102 84-85 -1.011743581599653 1102 86-87 -0.6755275917202894 1102 88-89 -0.7685769313319 1102 90-91 -1.2961395018380344 1103 1 0.28928383987493334 1103 2 0.23156368438936425 1103 3 0.3024070676506341 1103 4 0.048480396613094 1103 5 0.09476377223883503 1103 6 0.1751098459715763 1103 7 0.031103642264980635 1103 8 0.09181259302977196 1103 9 0.049269563108872205 1103 10-11 0.09516737026751798 1103 12-13 0.13183207961454713 1103 14-15 0.043364522029726515 1103 16-17 0.02345988392712428 1103 18-19 -0.03170083847314942 1103 20-21 0.08321902221158695 1103 22-23 0.05180926103017924 1103 24-25 0.025016232996627252 1103 26-27 0.01837770300252828 1103 28-29 0.0014046127978986078 1103 30-31 0.06860075622552841 1103 32-33 0.12187523892340835 1103 34-35 0.06482835838890821 1103 36-37 0.07368854315232198 1103 38-39 -0.0382126213611258 1103 40-41 0.14268712173821996 1103 42-43 0.15932057660850063 1103 44-45 0.0012580884036097473 1103 46-47 -0.03027832560292154 1103 48-49 -0.045789994164437076 1103 50-51 0.027009293606987228 1103 52-53 -0.043550675139705675 1103 54-55 0.19032837325142538 1103 56-57 0.08640564570844589 1103 58-59 0.06241271063771592 1103 60-61 0.2461206441045718 1103 62-63 -0.12201042866053058 1103 64-65 0.06892068518934735 1103 66-67 -0.12248432748739901 1103 68-69 0.14713067215696896 1103 70-71 -0.07730290397448414 1103 72-73 0.08421104349736197 1103 74-75 0.09733071719553976 1103 76-77 0.24063155383521462 1103 78-79 -0.051762542448813065 1103 80-81 0.07787609365878723 1103 82-83 0.4827401618634255 1103 84-85 0.6069905956155353 1103 86-87 0.678639074946382 1103 88-89 0.6879448077985373 1103 90-91 0.43084462514609356 1104 1 0.16837101608779648 1104 2 0.008723274433286576 1104 3 0.2109397463459075 1104 4 -0.024453035062727224 1104 5 0.0405007354374618 1104 6 0.04066979269049398 1104 7 0.18680328669083224 1104 8 -0.10301121612708641 1104 9 0.045020286916930274 1104 10-11 -0.04026124519500485 1104 12-13 -0.05151868382406377 1104 14-15 -0.07069441741258942 1104 16-17 -0.03639106115152657 1104 18-19 0.03547295251970439 1104 20-21 -0.05035576394853791 1104 22-23 0.00548994884048426 1104 24-25 -0.08817743932473832 1104 26-27 0.03272176227222445 1104 28-29 -0.10798892962331053 1104 30-31 -0.12140133538710529 1104 32-33 -0.11098796186845306 1104 34-35 -0.013972519770064196 1104 36-37 -0.1384590966714896 1104 38-39 -0.06214230843605151 1104 40-41 -0.0051223418055883485 1104 42-43 0.11674521780216907 1104 44-45 0.031990850282412 1104 46-47 -0.1427492340329195 1104 48-49 -0.04910491580029941 1104 50-51 -0.034308225484402044 1104 52-53 0.02384277534286383 1104 54-55 -0.07191893957689643 1104 56-57 -0.023059577589933156 1104 58-59 0.014868063873556991 1104 60-61 -0.11772969056973892 1104 62-63 0.09504135736338526 1104 64-65 -0.16453146491455328 1104 66-67 -0.05798900863133838 1104 68-69 0.020526498361661538 1104 70-71 -0.2573333913148872 1104 72-73 -0.08557024965399052 1104 74-75 -0.3153350484113986 1104 76-77 0.03683651166808488 1104 78-79 -0.3548364475710528 1104 80-81 -0.36027758851403746 1104 82-83 0.1520791887893722 1104 84-85 -0.13726518328564197 1104 86-87 0.08604648235378676 1104 88-89 0.6879448077985373 1104 90-91 0.8308446251460921 1201 1 0.21559962934861687 1201 2 0.20299225581793934 1201 3 0.30030180449274013 1201 4 0.010585659770988798 1201 5 0.03927505043431978 1201 6 -0.14233376305097778 1201 7 0.07967507083640868 1201 8 0.019331390022252037 1201 9 0.08581091649233485 1201 10-11 0.055768874026917103 1201 12-13 0.03183207961455281 1201 14-15 0.024191589698894234 1201 16-17 -0.10548748449392775 1201 18-19 0.09859991340655228 1201 20-21 0.010512255294294448 1201 22-23 -0.0406719419773367 1201 24-25 -0.022277000086084797 1201 26-27 0.008528078942376283 1201 28-29 0.03433694362496453 1201 30-31 0.0314578990826746 1201 32-33 0.03102309301885242 1201 34-35 -0.06320921746600305 1201 36-37 0.10051124740039796 1201 38-39 -0.07886079260975976 1201 40-41 -0.0894905396729655 1201 42-43 -0.047774463326746286 1201 44-45 0.015315837673320232 1201 46-47 -0.05443336228326956 1201 48-49 -0.08458426594546609 1201 50-51 0.015046765689049835 1201 52-53 -0.03886190335834527 1201 54-55 0.031493356494230795 1201 56-57 0.09512870676636709 1201 58-59 0.09142696937432504 1201 60-61 0.0698646360662778 1201 62-63 -0.17397753023290363 1201 64-65 -0.18956039777085465 1201 66-67 -0.06764571310512224 1201 68-69 -0.1484473029919826 1201 70-71 -0.22469378212025504 1201 72-73 -0.10824351417507927 1201 74-75 0.26862913555278567 1201 76-77 0.1157480762939258 1201 78-79 -0.1287337336761354 1201 80-81 -0.12303674849116675 1201 82-83 0.2502469125384934 1201 84-85 -0.14981685161321678 1201 86-87 0.06842886473616971 1201 88-89 -0.11205519220145987 1201 90-91 -0.7691553748539093 1202 1 0.08014399862836541 1202 2 0.17139655347014582 1202 3 0.21891700544777137 1202 4 0.06193464504755042 1202 5 0.03640199034677494 1202 6 0.07757073356660982 1202 7 -0.10769460683967935 1202 8 0.03359322846236523 1202 9 0.04185548473746081 1202 10-11 0.07138367705119464 1202 12-13 0.0061655455914646495 1202 14-15 -0.0044395284865359486 1202 16-17 0.11518501849055696 1202 18-19 -0.023218630166866205 1202 20-21 0.030118303841845773 1202 22-23 0.01507815750535002 1202 24-25 7.59211613079458E-4 1202 26-27 0.0015284768731405052 1202 28-29 0.04922751266595071 1202 30-31 -0.03275220835863735 1202 32-33 0.0016201933038146876 1202 34-35 0.005887311574312548 1202 36-37 0.14104890021592809 1202 38-39 0.01362326179695117 1202 40-41 0.00513388214601207 1202 42-43 0.15846799537629863 1202 44-45 0.07148750014992089 1202 46-47 0.20926929925105497 1202 48-49 0.030710353718525596 1202 50-51 -0.006134709685575501 1202 52-53 0.13601898699742065 1202 54-55 -0.033072974416022305 1202 56-57 -0.16560687739504232 1202 58-59 0.1304422088584758 1202 60-61 -0.04049504820221017 1202 62-63 0.10175516370954796 1202 64-65 -0.023931927783131357 1202 66-67 0.12690626657406767 1202 68-69 0.03156086952952819 1202 70-71 -0.0016459807307427354 1202 72-73 0.25048195955811536 1202 74-75 0.04686275378639948 1202 76-77 -0.4669480006069975 1202 78-79 -0.30721927693456763 1202 80-81 0.18693141065803331 1202 82-83 -0.36780431514151957 1202 84-85 0.01113072127464676 1202 86-87 -0.5110075153970755 1202 88-89 -0.3550659448896383 1202 90-91 -0.9103318454421441 1203 1 0.2898373580300131 1203 2 0.16256438417942576 1203 3 0.21031679999408937 1203 4 0.18075860788655973 1203 5 0.08297993896776035 1203 6 0.004344233550042986 1203 7 0.3193371722059979 1203 8 -0.13481836505122402 1203 9 0.04999566106896225 1203 10-11 -0.08134599151342314 1203 12-13 0.002890762009833736 1203 14-15 0.0650843218792474 1203 16-17 0.10755360317977392 1203 18-19 0.08750024330758066 1203 20-21 -0.029638699419287207 1203 22-23 0.029417031330673638 1203 24-25 -0.04651072996711747 1203 26-27 0.017246463427035508 1203 28-29 0.02734104239533508 1203 30-31 -0.02289179600879976 1203 32-33 0.1060295771704105 1203 34-35 0.027272569137295477 1203 36-37 -0.10839489516799716 1203 38-39 0.0016837830544744747 1203 40-41 0.02966037007762168 1203 42-43 -0.06633688951411898 1203 44-45 0.06077300567360311 1203 46-47 -0.036338719251354235 1203 48-49 0.01913829298244707 1203 50-51 0.08515538517554688 1203 52-53 -0.007943822413423618 1203 54-55 -0.061208845485680285 1203 56-57 0.09675161240940611 1203 58-59 0.08124724564723351 1203 60-61 0.1809071911546951 1203 62-63 -0.06423770779802851 1203 64-65 -0.203323924701742 1203 66-67 -0.13646559943936154 1203 68-69 -0.38972908440685927 1203 70-71 -0.08582624841062625 1203 72-73 -0.1708626550550889 1203 74-75 -0.1649572458596822 1203 76-77 -0.3026506321520799 1203 78-79 0.08174592706628658 1203 80-81 -0.1083918068948293 1203 82-83 -0.5029202303740661 1203 84-85 0.12552104114441676 1203 86-87 -0.10881031776616368 1203 88-89 -0.22872185886813412 1203 90-91 -0.16915537485390786 1204 1 0.28099380371983074 1204 2 0.22474392099340434 1204 3 -0.014477000557462816 1204 4 0.2045134855986106 1204 5 -0.060482380721857965 1204 6 0.15597223158069085 1204 7 0.09051312630911923 1204 8 0.103645934211535 1204 9 0.028371807236936775 1204 10-11 -0.0492793415014674 1204 12-13 -0.1074958853919128 1204 14-15 -0.04294729090553773 1204 16-17 -0.07659296217560296 1204 18-19 0.0063849815047731795 1204 20-21 -0.0614024479565316 1204 22-23 -0.10148315581569278 1204 24-25 -0.1492011476156634 1204 26-27 -0.03152262192251243 1204 28-29 -0.07153242708783125 1204 30-31 -0.05463216951261529 1204 32-33 -0.06684044365137964 1204 34-35 -0.07195814386881239 1204 36-37 -0.05483730429985911 1204 38-39 -0.08307719399523705 1204 40-41 -0.016062518687149918 1204 42-43 -0.10903226153835988 1204 44-45 -0.022195120849396233 1204 46-47 -0.09631494689336506 1204 48-49 -0.19581955613655566 1204 50-51 -0.06263549793168721 1204 52-53 -0.16388305634253442 1204 54-55 -0.2180805287439469 1204 56-57 -0.19509643468779103 1204 58-59 -0.10668619150685998 1204 60-61 0.02344877728007333 1204 62-63 0.0020660820443296757 1204 64-65 0.016257763397341307 1204 66-67 0.16772766637693337 1204 68-69 -0.32959879194514485 1204 70-71 -0.05127361527260632 1204 72-73 0.16375225826324424 1204 74-75 -0.007029141120725058 1204 76-77 -0.17240387961651038 1204 78-79 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54-55 0.03589610672471366 2401 56-57 0.09544051893848149 2401 58-59 -0.09047376012490105 2401 60-61 0.06904397189992295 2401 62-63 -0.020958753461407298 2401 64-65 -0.001493957726204087 2401 66-67 0.1436679824183571 2401 68-69 -0.011537491309354664 2401 70-71 0.019604807974936023 2401 72-73 0.1193777622167218 2401 74-75 0.21669655138065025 2401 76-77 0.14147049035979364 2401 78-79 0.18304377042397135 2401 80-81 0.1359120807992511 2401 82-83 0.12454508835799771 2401 84-85 0.07090432374092615 2401 86-87 0.37151376782107803 2401 88-89 -0.17183947571456315 2401 90-91 -0.11787332357185676 2402 1 0.36486531693206103 2402 2 0.3099793497209191 2402 3 0.19265605460400081 2402 4 0.28017177459074816 2402 5 0.2919764300515908 2402 6 0.11212996636601957 2402 7 0.20769465250084806 2402 8 0.15994554222518786 2402 9 0.14244643051102202 2402 10-11 0.042221922536036516 2402 12-13 0.1691262496189978 2402 14-15 0.15879328084263733 2402 16-17 -0.010049122233134256 2402 18-19 -0.004412992690468798 2402 20-21 0.025256358543067847 2402 22-23 0.08923460007873985 2402 24-25 0.036872977662021356 2402 26-27 0.0878738733349067 2402 28-29 0.03922345897876767 2402 30-31 -0.01008193180295791 2402 32-33 0.06285322090728584 2402 34-35 0.08996265504621448 2402 36-37 0.06221425644167766 2402 38-39 0.11675749116248824 2402 40-41 0.03572570667746788 2402 42-43 -0.06936484026289236 2402 44-45 -0.004456954346231612 2402 46-47 0.07617591295509385 2402 48-49 0.10653589807807862 2402 50-51 0.18675183734324463 2402 52-53 0.05838037854091027 2402 54-55 0.1762846546611243 2402 56-57 0.008981075091206492 2402 58-59 0.08178130483081958 2402 60-61 0.19140902362608614 2402 62-63 0.03592960171341275 2402 64-65 0.16084090457498945 2402 66-67 0.2579668378773263 2402 68-69 0.10083095737899583 2402 70-71 0.12238893788845928 2402 72-73 0.3580048320041911 2402 74-75 0.190730638683263 2402 76-77 0.2932705356593601 2402 78-79 0.10248092964160804 2402 80-81 0.13599947127369205 2402 82-83 -0.04535013012806388 2402 84-85 0.20845991860384316 2402 86-87 0.2138123474095508 2402 88-89 0.20518618710888603 2402 90-91 0.4672082615097324 2403 1 -0.3407913480949887 2403 2 -0.11405035069835634 2403 3 0.010677744342359574 2403 4 0.11264079761560453 2403 5 0.2030344489305662 2403 6 0.20192688857809316 2403 7 0.06418634903189258 2403 8 0.20459454791699017 2403 9 0.10616179368531675 2403 10-11 0.09090671863844335 2403 12-13 0.02819799440151627 2403 14-15 0.073940963132479 2403 16-17 0.05040223981684733 2403 18-19 0.11804853496042966 2403 20-21 0.0735698994045677 2403 22-23 0.12085688007780249 2403 24-25 0.09193352622970252 2403 26-27 0.09394161277696611 2403 28-29 0.12245724437684657 2403 30-31 0.11784887652627418 2403 32-33 0.08746108750997905 2403 34-35 0.16635091750438846 2403 36-37 0.04359074184866785 2403 38-39 0.1425311937302638 2403 40-41 0.14898570077546935 2403 42-43 0.1156227580615905 2403 44-45 0.05312892463069119 2403 46-47 0.14424404779500577 2403 48-49 0.0015318215926001244 2403 50-51 0.21323801086848704 2403 52-53 0.10885537102827669 2403 54-55 0.13666920304788022 2403 56-57 0.05786386111855535 2403 58-59 0.17104360776865946 2403 60-61 -0.018385158940816382 2403 62-63 0.18403401583920953 2403 64-65 0.16844920551638864 2403 66-67 0.17059145761768946 2403 68-69 0.3464642001052596 2403 70-71 0.1705334637177387 2403 72-73 0.3524010521327412 2403 74-75 0.20007702657372306 2403 76-77 0.0991877459504309 2403 78-79 0.3796740513048249 2403 80-81 -0.024171408388717452 2403 82-83 0.12149788378946624 2403 84-85 0.14750875484894266 2403 86-87 0.44553096683826965 2403 88-89 0.3370014115721247 2403 90-91 0.49751129181276355 2404 1 -0.3111057851512271 2404 2 -0.24766543766630633 2404 3 -0.33657797525641797 2404 4 -0.07154343169428756 2404 5 -0.22969405326711723 2404 6 -0.1279991041338775 2404 7 -0.21166172940831984 2404 8 -0.0801118647957324 2404 9 -0.02797923340060038 2404 10-11 0.02337976420739807 2404 12-13 -0.024240113716743394 2404 14-15 0.08089088611981765 2404 16-17 0.04543022734456059 2404 18-19 0.08633622420496323 2404 20-21 0.018866492063949636 2404 22-23 -0.033422018453322266 2404 24-25 0.052048481712631656 2404 26-27 -0.03251505351402528 2404 28-29 -0.03269884713674287 2404 30-31 -0.010444823462457009 2404 32-33 -0.03874225400918618 2404 34-35 -0.08130235307214662 2404 36-37 0.031567286897363545 2404 38-39 0.11691889199701677 2404 40-41 0.068932253640547 2404 42-43 0.06033963151865862 2404 44-45 0.07267556224899607 2404 46-47 0.0937447400526068 2404 48-49 0.047762253293029744 2404 50-51 0.022955174583920268 2404 52-53 0.058276374830789734 2404 54-55 0.037186329969593146 2404 56-57 0.1024878966959406 2404 58-59 0.19061283734642842 2404 60-61 0.29317852952730306 2404 62-63 0.11399326901084095 2404 64-65 0.25588384384639795 2404 66-67 0.2472051004397784 2404 68-69 0.3187202596875025 2404 70-71 0.15540377670366468 2404 72-73 0.06919406961385732 2404 74-75 0.3678621155215822 2404 76-77 0.16062757848240494 2404 78-79 0.383058168295463 2404 80-81 0.022313282282020452 2404 82-83 0.15205188722482177 2404 84-85 0.00251507835479714 2404 86-87 0.04284719387148073 2404 88-89 -0.42173261155630115 2404 90-91 -0.05804426374279359 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 13.0 31 310.0 32 2150.0 33 4011.0 34 5342.0 35 6678.0 36 9450.0 37 14387.0 38 37869.0 39 228479.0 40 246709.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.132713470845818 12.068773046784974 33.132454194514864 28.666059287854345 2 26.058256552826847 18.45180969117941 30.790948753175805 24.698985002817935 3 26.247531045360343 19.437987601661515 23.3350198146551 30.97946153832304 4 26.125362127047403 21.231042907891442 21.936481700543215 30.707113264517943 5 26.371671537039138 21.430899227402282 21.90533292281765 30.29209631274093 6 27.012652165380203 23.828814960055745 24.3504219489052 24.80811092565885 7 25.31405350027638 23.556938345225685 24.204040698668887 26.92496745582905 8 24.365726261660537 24.632201354341653 24.679554698514043 26.32251768548377 9 24.369867428641392 24.66010921877785 25.034434703699503 25.935588648881254 10-11 24.030111685472967 23.519037664813943 25.033264373900565 27.41758627581252 12-13 24.535874209352198 23.280290385830725 24.640663739041663 27.543171665775418 14-15 24.96880620958986 22.962230756627218 24.49950396021599 27.569459073566932 16-17 24.9470200702558 22.76309464006957 24.34781121319988 27.94207407647475 18-19 25.05874155336974 23.006973365094282 24.144263853553934 27.79002122798205 20-21 25.222047573006073 23.134269237071006 23.992030954322928 27.651652235599993 22-23 24.907408907830224 22.71322058556101 24.28488348016471 28.094487026444053 24-25 25.029393283027158 23.067200337054985 24.337368270378594 27.56603810953927 26-27 25.180185776351777 23.13787025183697 24.237530135992323 27.44441383581893 28-29 25.162990930844316 22.81701983618984 24.354923217362654 27.665066015603195 30-31 25.316486464588266 23.01389101450319 24.31188613509304 27.3577363858155 32-33 25.430146053885537 23.003209934914786 24.344715242942947 27.22192876825674 34-35 25.206764987763425 22.786504177511375 24.557377583902788 27.449353250822405 36-37 25.25324512604621 23.098882373577908 24.59710319250238 27.0507693078735 38-39 25.465802544780004 23.042513365043067 24.554305554678237 26.93737853549869 40-41 25.257580643367838 22.81976582567022 24.669698553367507 27.252954977594428 42-43 25.446879492865165 23.05982059845407 24.656666835995136 26.836633072685622 44-45 25.551060364652912 23.109528332948575 24.61665777300085 26.722753529397664 46-47 25.35409346118237 22.83131290708566 24.813749005127317 27.000844626604653 48-49 25.65432326075171 23.12894453431297 24.602203897809986 26.614528307125333 50-51 25.61684151771556 23.259048066067056 24.5466452701417 26.577465146075685 52-53 25.597331590359694 22.94828832380577 24.816797562603533 26.637582523231 54-55 25.633332448834928 23.21467155415215 24.686034654647596 26.465961342365325 56-57 25.89173091862903 23.123397181545556 24.51737935528063 26.46749254454478 58-59 25.614822795550758 22.92016840722921 24.774636262117852 26.69037253510218 60-61 25.8971739695397 23.222184197593126 24.585667345106103 26.294974487761074 62-63 25.955332268167897 23.141103657516627 24.506399644688496 26.39716442962698 64-65 26.035260485945567 23.020459667688943 24.397771584120097 26.546508262245393 66-67 26.186215217944792 23.25347128693143 24.347161902372246 26.213151592751533 68-69 26.252855315214134 23.106414061873256 24.295829656433273 26.34490096647934 70-71 26.170457648940737 23.032655768405448 24.406968124536693 26.389918458117123 72-73 26.176270030685306 23.11683145812024 24.103875440390954 26.6030230708035 74-75 26.43196329881419 23.15286795085046 24.011375221333907 26.40379352900145 76-77 26.564864713371716 22.909443238871585 23.996060312452457 26.529631735304243 78-79 26.668419054294812 23.163643798008117 23.805212379645045 26.362724768052026 80-81 27.11280793533811 23.0267370633933 23.518467058923026 26.34198794234556 82-83 27.379438672662427 22.077841525817956 23.55559001395565 26.987129787563962 84-85 27.765966316491582 22.242371185592795 22.892696348174088 27.09896614974154 86-87 28.55979244599976 21.219983106069748 22.321105345722213 27.89911910220828 88-89 27.738939378807547 20.188754618995304 23.06501547987616 29.007290522320982 90-91 25.29346348696404 21.079945632027677 26.61559372297047 27.01099715803781 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 2.5 8 5.0 9 5.5 10 4.0 11 4.5 12 9.5 13 19.5 14 33.5 15 48.0 16 64.0 17 94.0 18 128.5 19 163.0 20 213.0 21 310.0 22 459.0 23 623.5 24 810.0 25 987.5 26 1215.5 27 1585.5 28 2018.0 29 2484.0 30 3095.0 31 3841.0 32 4704.0 33 5614.5 34 6802.5 35 8256.5 36 9789.0 37 11393.0 38 13285.0 39 15662.0 40 18231.0 41 20936.0 42 23519.5 43 25886.5 44 28645.0 45 31512.5 46 34089.5 47 36667.5 48 38820.0 49 40408.0 50 41685.0 51 42562.0 52 43037.0 53 42951.0 54 42455.5 55 41781.5 56 40690.0 57 39236.5 58 37461.0 59 35404.0 60 33387.5 61 31441.0 62 29476.5 63 27407.5 64 25093.5 65 22681.0 66 20226.0 67 18041.5 68 15951.5 69 13724.5 70 11639.5 71 9713.0 72 7946.0 73 6262.0 74 4872.5 75 3875.0 76 3015.0 77 2208.0 78 1618.0 79 1166.5 80 773.0 81 546.0 82 394.5 83 264.0 84 175.5 85 114.5 86 75.5 87 51.0 88 33.0 89 18.0 90 12.0 91 9.5 92 6.5 93 5.5 94 6.0 95 4.5 96 1.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012603551680863668 2 0.005041420672345467 3 3.601014765961048E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9090.0 32-33 19807.0 34-35 21988.0 36-37 23401.0 38-39 24924.0 40-41 26285.0 42-43 26822.0 44-45 26975.0 46-47 27250.0 48-49 26822.0 50-51 26397.0 52-53 25689.0 54-55 24509.0 56-57 23544.0 58-59 22165.0 60-61 21244.0 62-63 20047.0 64-65 18535.0 66-67 17364.0 68-69 16226.0 70-71 14813.0 72-73 13724.0 74-75 12446.0 76-77 11358.0 78-79 10230.0 80-81 9307.0 82-83 8535.0 84-85 7586.0 86-87 6722.0 88-89 6152.0 90-91 5442.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.83822689135943 #Duplication Level Percentage of deduplicated Percentage of total 1 99.84530792357201 99.68378506511229 2 0.14800698628233278 0.29553510155923723 3 0.006027018637023308 0.018051805624847546 4 6.580715086403119E-4 0.0026280277036148264 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016204566446824716 0.0 0.0 0.0 0.0 2 0.0016204566446824716 0.0 0.0 0.0 0.0 3 0.0016204566446824716 0.0 0.0 0.0 0.0 4 0.0016204566446824716 0.0 0.0 0.0 0.0 5 0.0016204566446824716 0.0 0.0 0.0 0.0 6 0.0019805581212785765 0.0 0.0 0.0 0.0 7 0.0027007610744707857 0.0 0.0 0.0 0.0 8 0.007742181746816253 0.0 0.0 0.0 0.0 9 0.008642435438306515 0.0 1.800507382980524E-4 0.0 0.0 10-11 0.00900253691490262 0.0 1.800507382980524E-4 0.0 9.00253691490262E-5 12-13 0.00900253691490262 0.0 1.800507382980524E-4 0.0 2.700761074470786E-4 14-15 0.00900253691490262 0.0 1.800507382980524E-4 0.0 3.601014765961048E-4 16-17 0.00900253691490262 0.0 1.800507382980524E-4 0.0 3.601014765961048E-4 18-19 0.00900253691490262 0.0 1.800507382980524E-4 0.0 3.601014765961048E-4 20-21 0.00900253691490262 0.0 3.601014765961048E-4 0.0 5.401522148941572E-4 22-23 0.009182587653200671 0.0 3.601014765961048E-4 0.0 5.401522148941572E-4 24-25 0.009542689129796778 0.0 3.601014765961048E-4 0.0 5.401522148941572E-4 26-27 0.009812765237243856 0.0 3.601014765961048E-4 0.0 5.401522148941572E-4 28-29 0.010262892082988986 0.0 3.601014765961048E-4 0.0 5.401522148941572E-4 30-31 0.010893069667032169 0.0 3.601014765961048E-4 0.0 7.202029531922096E-4 32-33 0.010983095036181195 0.0 3.601014765961048E-4 0.0 7.202029531922096E-4 34-35 0.011163145774479247 0.0 3.601014765961048E-4 0.0 7.202029531922096E-4 36-37 0.011523247251075354 0.0 3.601014765961048E-4 0.0 7.202029531922096E-4 38-39 0.01161327262022438 0.0 3.601014765961048E-4 0.0 7.202029531922096E-4 40-41 0.011793323358522432 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 42-43 0.012153424835118536 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 44-45 0.01251352631171464 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 46-47 0.012963653157459773 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 48-49 0.012963653157459773 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 50-51 0.013053678526608799 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 52-53 0.013143703895757825 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 54-55 0.01395393221809906 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 56-57 0.014043957587248086 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 58-59 0.014314033694695165 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 60-61 0.014584109802142243 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 62-63 0.014854185909589323 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 64-65 0.015034236647887375 1.800507382980524E-4 3.601014765961048E-4 0.0 7.202029531922096E-4 66-67 0.015214287386185427 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