##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-14B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 577404 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26503453387922 40.0 40.0 40.0 40.0 40.0 2 39.15765737681069 40.0 40.0 40.0 40.0 40.0 3 39.15789637757965 40.0 40.0 40.0 40.0 40.0 4 39.071679448012134 40.0 40.0 40.0 40.0 40.0 5 39.21579344791515 40.0 40.0 40.0 40.0 40.0 6 39.28167452944559 40.0 40.0 40.0 40.0 40.0 7 39.304951126074634 40.0 40.0 40.0 40.0 40.0 8 39.30427915289815 40.0 40.0 40.0 40.0 40.0 9 39.342249101149285 40.0 40.0 40.0 40.0 40.0 10-11 39.33017783042722 40.0 40.0 40.0 40.0 40.0 12-13 39.32768044558057 40.0 40.0 40.0 40.0 40.0 14-15 39.33062119417254 40.0 40.0 40.0 40.0 40.0 16-17 39.47393506106643 40.0 40.0 40.0 40.0 40.0 18-19 39.48530318459865 40.0 40.0 40.0 40.0 40.0 20-21 39.492348511614054 40.0 40.0 40.0 40.0 40.0 22-23 39.490017388171886 40.0 40.0 40.0 40.0 40.0 24-25 39.489168762253115 40.0 40.0 40.0 40.0 40.0 26-27 39.47570851604769 40.0 40.0 40.0 40.0 40.0 28-29 39.47253915802454 40.0 40.0 40.0 40.0 40.0 30-31 39.467454543951234 40.0 40.0 40.0 40.0 40.0 32-33 39.46367666327079 40.0 40.0 40.0 40.0 40.0 34-35 39.461062859959256 40.0 40.0 40.0 40.0 40.0 36-37 39.44496975398737 40.0 40.0 40.0 40.0 40.0 38-39 39.43581873162478 40.0 40.0 40.0 40.0 40.0 40-41 39.42870764282263 40.0 40.0 40.0 40.0 40.0 42-43 39.42723867677045 40.0 40.0 40.0 40.0 40.0 44-45 39.39645380538718 40.0 40.0 40.0 40.0 40.0 46-47 39.39845513538875 40.0 40.0 40.0 40.0 40.0 48-49 39.38905583535204 40.0 40.0 40.0 40.0 40.0 50-51 39.39008115403332 40.0 40.0 40.0 40.0 40.0 52-53 39.39042981119161 40.0 40.0 40.0 40.0 40.0 54-55 39.36265235409472 40.0 40.0 40.0 40.0 40.0 56-57 39.35512731341662 40.0 40.0 40.0 40.0 40.0 58-59 39.344899207542625 40.0 40.0 40.0 40.0 40.0 60-61 39.30658762738648 40.0 40.0 40.0 40.0 40.0 62-63 39.37944070518897 40.0 40.0 40.0 40.0 40.0 64-65 39.377091640240025 40.0 40.0 40.0 40.0 40.0 66-67 39.3728258103354 40.0 40.0 40.0 40.0 40.0 68-69 39.370578801276004 40.0 40.0 40.0 40.0 40.0 70-71 39.37540759959569 40.0 40.0 40.0 40.0 40.0 72-73 39.37839476860448 40.0 40.0 40.0 40.0 40.0 74-75 39.364583517734644 40.0 40.0 40.0 40.0 40.0 76-77 39.35955582585714 40.0 40.0 40.0 40.0 40.0 78-79 39.35272620128293 40.0 40.0 40.0 40.0 40.0 80-81 39.34842173289418 40.0 40.0 40.0 40.0 40.0 82-83 39.32894658279 40.0 40.0 40.0 40.0 40.0 84-85 39.33420015507073 40.0 40.0 40.0 40.0 40.0 86-87 39.33953910020149 40.0 40.0 40.0 40.0 40.0 88-89 39.31853193120239 40.0 40.0 40.0 40.0 40.0 90-91 39.38762089304022 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17192595801110855 1101 2 0.3044789546924278 1101 3 0.41575379895963493 1101 4 0.15098032153734664 1101 5 0.08747791274521433 1101 6 0.13297601304414286 1101 7 0.21308041743527895 1101 8 0.14496795421224817 1101 9 0.08891646319867164 1101 10-11 0.0174134695096555 1101 12-13 0.011127794776854216 1101 14-15 0.05765655250235824 1101 16-17 0.08734286458121687 1101 18-19 0.0947966832532785 1101 20-21 0.037737247045996014 1101 22-23 -0.004226723802148058 1101 24-25 0.04597830776842926 1101 26-27 0.0943891612562382 1101 28-29 0.026727360774302156 1101 30-31 0.11025414630238828 1101 32-33 0.02941640494019282 1101 34-35 0.06683879908587897 1101 36-37 0.08038719449911014 1101 38-39 0.06521843613853662 1101 40-41 0.08448295584216936 1101 42-43 0.0438182450137532 1101 44-45 0.05229606646708618 1101 46-47 0.13880561630829646 1101 48-49 0.12332405410406722 1101 50-51 0.07970951129135528 1101 52-53 0.13696490764425562 1101 54-55 0.1933306111807127 1101 56-57 -0.026297158640765872 1101 58-59 0.19983414750625172 1101 60-61 0.19074498802297057 1101 62-63 -0.004703357257234586 1101 64-65 0.06136779215774624 1101 66-67 0.0618524084465335 1101 68-69 0.014448563274250148 1101 70-71 -0.04381977206397636 1101 72-73 0.0185186206735537 1101 74-75 0.15390498632579153 1101 76-77 0.2695522315336518 1101 78-79 0.04548183124543215 1101 80-81 0.13374832179069074 1101 82-83 -0.008163314698983015 1101 84-85 0.04668868474996657 1101 86-87 0.17960263985527547 1101 88-89 0.7182917986335084 1101 90-91 0.7935349628348405 1102 1 0.3248774505684935 1102 2 0.14055317102582876 1102 3 0.035688275986252904 1102 4 -6.15523294790421E-4 1102 5 0.10946953139935545 1102 6 0.048280307542469814 1102 7 0.05939730547061117 1102 8 0.08131706577882625 1102 9 0.09500611885338373 1102 10-11 -0.0542314368591974 1102 12-13 0.03949956502683705 1102 14-15 -0.03126748280903513 1102 16-17 0.19003855932377434 1102 18-19 0.0085839433923951 1102 20-21 -0.04650657588058493 1102 22-23 0.06356642156955417 1102 24-25 0.022695596042609623 1102 26-27 -0.036452362448763154 1102 28-29 -0.02785359883062455 1102 30-31 -0.05929865133491319 1102 32-33 -0.04348768250785895 1102 34-35 -0.046795593877575925 1102 36-37 -0.0621011701684111 1102 38-39 -0.03214824840627273 1102 40-41 0.12270044722794893 1102 42-43 -0.05035766057346791 1102 44-45 -0.003784257868289842 1102 46-47 -0.059600001000639224 1102 48-49 0.08829653312344732 1102 50-51 -0.0819197602941486 1102 52-53 -0.07658993059886399 1102 54-55 -0.2237997935272773 1102 56-57 -0.133825271055926 1102 58-59 -0.07787087576316765 1102 60-61 -0.11370027684429118 1102 62-63 -0.05401009033369775 1102 64-65 0.13823569871477304 1102 66-67 -0.10183556787154657 1102 68-69 0.004992871612735428 1102 70-71 0.006590704260780456 1102 72-73 0.04758848892848988 1102 74-75 0.1739780140974716 1102 76-77 0.027914752073357363 1102 78-79 0.2593963806538966 1102 80-81 0.05433278769069716 1102 82-83 0.18744226026896627 1102 84-85 0.5732479363092153 1102 86-87 0.7462693065219383 1102 88-89 0.7182917986335084 1102 90-91 -0.8064650371651609 1103 1 0.2004719492484881 1103 2 0.09985387412594093 1103 3 -0.014545720274746543 1103 4 0.10602058597097397 1103 5 0.10599428561067725 1103 6 0.10388028877337518 1103 7 0.13143703667743978 1103 8 -0.005789308057053688 1103 9 0.051813496094872846 1103 10-11 0.08617323466932447 1103 12-13 -0.02265972036462216 1103 14-15 0.10547128845745846 1103 16-17 0.02445761884403197 1103 18-19 -0.057669967864363514 1103 20-21 0.010158304429396026 1103 22-23 -0.03514419111785827 1103 24-25 0.02701051179160885 1103 26-27 -0.045125659215294434 1103 28-29 0.04151598190241401 1103 30-31 0.046085855766477835 1103 32-33 0.008644581058405265 1103 34-35 -0.10854715285186245 1103 36-37 -0.07128179339274965 1103 38-39 -0.0707368561927737 1103 40-41 -0.014466657098957114 1103 42-43 0.039679432247254454 1103 44-45 -0.02061955616672151 1103 46-47 0.0766327558110973 1103 48-49 -0.029742025835382435 1103 50-51 -0.14743782593173904 1103 52-53 0.09020744078265608 1103 54-55 -0.08169699502067829 1103 56-57 -0.1902969964872412 1103 58-59 -0.11384123034429905 1103 60-61 -0.131667116525243 1103 62-63 -0.052418710835816285 1103 64-65 -0.058279094103113493 1103 66-67 -0.22395096031264927 1103 68-69 -0.12722793137107402 1103 70-71 0.05047291951192534 1103 72-73 0.06802338704579114 1103 74-75 -0.10576021206932751 1103 76-77 0.2717236289358098 1103 78-79 -0.39808738741794514 1103 80-81 -0.3202641005695597 1103 82-83 -0.6474977919179423 1103 84-85 -0.8139969616499627 1103 86-87 -0.289628129375501 1103 88-89 -0.28170820136649155 1103 90-91 -2.806465037165161 1104 1 0.14653549847115954 1104 2 0.29713476845972764 1104 3 0.22195441840716512 1104 4 0.016096414419095595 1104 5 0.08467205936641164 1104 6 0.22297185174201672 1104 7 0.12954344096106496 1104 8 -0.03137071433864236 1104 9 0.02495715645112284 1104 10-11 -0.06730580085675086 1104 12-13 -0.061348682694863044 1104 14-15 -0.02566444587501593 1104 16-17 -0.037416138307484914 1104 18-19 -0.006803323449489085 1104 20-21 -0.03637582307334242 1104 22-23 0.06610300586216056 1104 24-25 0.011053160004962592 1104 26-27 -0.09528150007427172 1104 28-29 -0.0835722809068642 1104 30-31 0.003104805826879442 1104 32-33 -0.01600405081090628 1104 34-35 0.014623228425939772 1104 36-37 0.017087194001128125 1104 38-39 -0.07340715346569482 1104 40-41 -0.011066609039538378 1104 42-43 0.05110085819853083 1104 44-45 -0.15997565534792102 1104 46-47 -0.149506009181124 1104 48-49 -0.056774934078113404 1104 50-51 0.158195879776045 1104 52-53 -0.053026539864994504 1104 54-55 -0.05589163706576272 1104 56-57 -0.09483469055478366 1104 58-59 0.07065964043970041 1104 60-61 0.06398514615499096 1104 62-63 -0.1380284262029292 1104 64-65 -0.03664004656793196 1104 66-67 -0.22373247363000104 1104 68-69 -0.43397504150878063 1104 70-71 -0.4107348545170737 1104 72-73 -0.508477001454601 1104 74-75 -0.3757569777557421 1104 76-77 -0.5684739787776465 1104 78-79 -0.673788566972334 1104 80-81 -0.016616761992644058 1104 82-83 -0.38629861375180496 1104 84-85 0.22117833923961427 1104 86-87 -0.3865150475696737 1104 88-89 -1.2243438602812162 1104 90-91 -1.984242814942938 1201 1 0.34126249827095734 1201 2 0.2682606467612558 1201 3 0.17298722319320348 1201 4 0.1757271977455943 1201 5 -0.004108848935274523 1201 6 0.11600112977095023 1201 7 0.0675393092173664 1201 8 -0.04077073724420188 1201 9 0.060738791261279346 1201 10-11 -0.01718458497358455 1201 12-13 0.14364997531898638 1201 14-15 0.07483851479998549 1201 16-17 0.06070709186630552 1201 18-19 0.03579315311559128 1201 20-21 0.16371125303169975 1201 22-23 0.11813272713995104 1201 24-25 0.028571543466796356 1201 26-27 0.037174719266587886 1201 28-29 0.02504554329207309 1201 30-31 0.0999673558453793 1201 32-33 0.08980452140278317 1201 34-35 0.18230399945333176 1201 36-37 0.0582703394733457 1201 38-39 0.0704954418352628 1201 40-41 0.10665516104224082 1201 42-43 0.0814372326075059 1201 44-45 -0.008378685414683673 1201 46-47 -0.08145819574712476 1201 48-49 -0.11785199280679137 1201 50-51 0.09454446470184763 1201 52-53 -0.1681299965002978 1201 54-55 0.1916089821440039 1201 56-57 0.12380404614668095 1201 58-59 -0.04197803040590031 1201 60-61 -0.07762173816952611 1201 62-63 -0.0546681756396552 1201 64-65 -0.03833540814953551 1201 66-67 -0.03364072747207558 1201 68-69 -0.2869318892561594 1201 70-71 0.011623761247122388 1201 72-73 0.06646331285895002 1201 74-75 -0.001191170000282682 1201 76-77 -0.18383803309177438 1201 78-79 0.10578900817668568 1201 80-81 0.00118387674049103 1201 82-83 -0.0916086346355911 1201 84-85 -0.595458357397078 1201 86-87 -0.5546202800819344 1201 88-89 -0.45342537308366104 1201 90-91 0.7935349628348405 1202 1 0.19886116071131 1202 2 0.019014765678662116 1202 3 0.019308934154921076 1202 4 0.1934620544107517 1202 5 -0.044080634786404005 1202 6 -0.08540609444445124 1202 7 -0.08665623921692145 1202 8 0.005739068043340012 1202 9 0.20644919566407793 1202 10-11 0.06857162388077853 1202 12-13 -0.06233943566711986 1202 14-15 -0.0689012532170139 1202 16-17 -0.03513219198196538 1202 18-19 0.013771313274425268 1202 20-21 -0.07496999675059612 1202 22-23 -0.03470871630983652 1202 24-25 -0.05272352079876441 1202 26-27 0.016374621736197525 1202 28-29 -0.04689008664975347 1202 30-31 -0.03289429032830071 1202 32-33 -0.042366352757582604 1202 34-35 0.02652937560151969 1202 36-37 0.02076847180445185 1202 38-39 0.01616823808031853 1202 40-41 0.06457617793290638 1202 42-43 0.02592983164950624 1202 44-45 -0.029427080546568618 1202 46-47 0.10141581357679996 1202 48-49 -0.08436891857932238 1202 50-51 -0.0682103506845877 1202 52-53 -0.06714433307227807 1202 54-55 -0.037458405794353666 1202 56-57 -0.13645071555729515 1202 58-59 -0.04197926784124917 1202 60-61 -0.07303364633759202 1202 62-63 -0.10494783181010803 1202 64-65 -0.005188404536710323 1202 66-67 -0.16663305483780277 1202 68-69 -0.11469009560146759 1202 70-71 0.033481711128828806 1202 72-73 -0.13598634984804647 1202 74-75 -0.17133420147776235 1202 76-77 -0.19841056774252763 1202 78-79 0.04559268871464894 1202 80-81 -0.17771583802129243 1202 82-83 -0.37740863102671796 1202 84-85 -0.1508466310952059 1202 86-87 -0.9394343142320736 1202 88-89 0.26114894149065293 1202 90-91 0.18483931066092651 1203 1 0.2035931742313437 1203 2 0.11660737104529062 1203 3 -0.04281771273753776 1203 4 -0.04734293972596504 1203 5 -0.011925706180484497 1203 6 0.0673565461018697 1203 7 0.029119438509503937 1203 8 0.008481014652979013 1203 9 0.061481271265932946 1203 10-11 0.07362956690347744 1203 12-13 0.007450865774558224 1203 14-15 -3.9570034400071563E-4 1203 16-17 -0.004032654146548964 1203 18-19 0.0617070930909307 1203 20-21 0.07546802490905691 1203 22-23 0.057932864706920384 1203 24-25 0.004577989858610465 1203 26-27 0.104872241933478 1203 28-29 0.06420876138776777 1203 30-31 0.1122547060157757 1203 32-33 0.017478493876431855 1203 34-35 0.08475777043445731 1203 36-37 0.044214963035202004 1203 38-39 0.012186623926858431 1203 40-41 -0.04089944634127818 1203 42-43 -0.03570163575087548 1203 44-45 0.04866365888518942 1203 46-47 -0.09448836487888457 1203 48-49 -0.10183829682869572 1203 50-51 -0.08736476887384725 1203 52-53 -0.06632836687458621 1203 54-55 -0.10898064259799867 1203 56-57 -0.22737860960747724 1203 58-59 -0.027754936926527307 1203 60-61 -0.025515017410427276 1203 62-63 0.06931926072032013 1203 64-65 0.023536556133592512 1203 66-67 0.07434424025928621 1203 68-69 0.23882002805228097 1203 70-71 0.15827089391603977 1203 72-73 -0.09517156115953185 1203 74-75 0.11811157987177268 1203 76-77 0.306031901744376 1203 78-79 0.12030554174046415 1203 80-81 0.038011955888315185 1203 82-83 0.0967621449116649 1203 84-85 -0.2739742859130132 1203 86-87 -0.36891575055173575 1203 88-89 -1.293785496052486 1203 90-91 -1.1559599866601076 1204 1 0.2970270816785501 1204 2 0.20411671588188085 1204 3 0.059660380584098505 1204 4 0.12847861918414338 1204 5 0.05049397500218333 1204 6 0.03523327165411416 1204 7 -0.021531064176791403 1204 8 -0.04965046028754472 1204 9 -0.007875064044242208 1204 10-11 -0.10727881500032055 1204 12-13 -0.11571386785401216 1204 14-15 -0.1027181527012786 1204 16-17 -0.04934203521431613 1204 18-19 -0.05564789503186773 1204 20-21 -0.13305599695551962 1204 22-23 -0.22562456043020518 1204 24-25 -0.09811783071371849 1204 26-27 -0.2557436200070313 1204 28-29 -0.09336708381496095 1204 30-31 -0.10022667872954116 1204 32-33 -0.05495025569472034 1204 34-35 -0.09160817011445488 1204 36-37 -0.04669175984824392 1204 38-39 -0.09619249954675269 1204 40-41 -0.20588577212389936 1204 42-43 -0.014763048993394534 1204 44-45 -0.2720017722099968 1204 46-47 -0.07054116791626797 1204 48-49 -0.11893933514548394 1204 50-51 -0.035871415513561544 1204 52-53 -0.11792885212408777 1204 54-55 0.07287005676882075 1204 56-57 -0.035089169499876505 1204 58-59 -0.19030702244157283 1204 60-61 -0.22434105387920056 1204 62-63 0.029957926081308983 1204 64-65 -0.2257244116149053 1204 66-67 -0.07010115719626242 1204 68-69 -0.15500303512326497 1204 70-71 -0.3255784594675788 1204 72-73 -0.49116755918608845 1204 74-75 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52-53 0.03276845173299847 2401 54-55 0.13813316295150457 2401 56-57 0.09910634014764952 2401 58-59 0.046980910758918526 2401 60-61 0.09778443406194981 2401 62-63 0.0689575986113411 2401 64-65 0.13558953531881457 2401 66-67 0.1817710558625123 2401 68-69 0.4232046595308887 2401 70-71 0.04756588204862311 2401 72-73 0.13671495527963629 2401 74-75 0.14305526226161192 2401 76-77 0.05114971464702478 2401 78-79 0.27118803638576594 2401 80-81 0.2589075490319246 2401 82-83 0.4830279447179677 2401 84-85 0.35458454502677483 2401 86-87 0.6212693065219383 2401 88-89 0.4602272825044764 2401 90-91 -0.09535392605404525 2402 1 0.3424052060097367 2402 2 0.27392983112005 2402 3 0.24841410448816248 2402 4 0.26794919819614194 2402 5 0.1339895406521947 2402 6 0.12172283900027026 2402 7 0.17024527382493915 2402 8 0.20398932331113429 2402 9 0.05937781350498028 2402 10-11 0.15127142894900913 2402 12-13 0.1691420458143611 2402 14-15 0.03776587757293015 2402 16-17 0.028837918479375446 2402 18-19 0.08815402932380323 2402 20-21 0.036521695442850444 2402 22-23 0.0015601967134557526 2402 24-25 0.059761395194925626 2402 26-27 0.0718884061973526 2402 28-29 0.05845661343378339 2402 30-31 0.0738099703233317 2402 32-33 0.12265100000313112 2402 34-35 0.08367162088543267 2402 36-37 0.06307926798901775 2402 38-39 0.15709396450189672 2402 40-41 0.10168255157083905 2402 42-43 0.00853303095977509 2402 44-45 0.16333497085372528 2402 46-47 0.17189805043245343 2402 48-49 0.019167417894578875 2402 50-51 0.08378204215070895 2402 52-53 0.08490391668335917 2402 54-55 0.021501914181442316 2402 56-57 0.04851175173163824 2402 58-59 0.2090700714839926 2402 60-61 0.023783744831042952 2402 62-63 0.28539930682541836 2402 64-65 0.11983326963991203 2402 66-67 -0.04218805780418222 2402 68-69 0.18112523555497972 2402 70-71 0.1027808055042243 2402 72-73 -0.05805465160533174 2402 74-75 0.041194630473754046 2402 76-77 0.19052646984742694 2402 78-79 0.4001328805298101 2402 80-81 0.47739157708611657 2402 82-83 0.4388250436083041 2402 84-85 0.15327747287631865 2402 86-87 0.19729285924040596 2402 88-89 -0.40380122462230617 2402 90-91 -0.8572586879588115 2403 1 -0.3090598804835949 2403 2 -0.1316840151717642 2403 3 -0.048408762956178464 2403 4 0.2533753644132659 2403 5 0.17657971883559043 2403 6 0.16922461531560629 2403 7 0.057784393002478396 2403 8 0.16073460774003934 2403 9 0.13244933290418004 2403 10-11 0.06944710136196619 2403 12-13 0.13138068601910646 2403 14-15 0.034048837132722554 2403 16-17 0.05270032934743085 2403 18-19 0.05202641974986477 2403 20-21 -0.004353102494093264 2403 22-23 0.1012316071193311 2403 24-25 0.017701237315556284 2403 26-27 0.015794782307118282 2403 28-29 0.1001672568393488 2403 30-31 0.05890961643992654 2403 32-33 0.14784088213734492 2403 34-35 0.034176176912048106 2403 36-37 0.08709348730524624 2403 38-39 0.021541419786046845 2403 40-41 0.037043015592409745 2403 42-43 -0.004685990144338348 2403 44-45 0.05832752971262778 2403 46-47 0.01446742285030922 2403 48-49 0.04421037252016191 2403 50-51 0.10443432061772029 2403 52-53 0.1310911922650888 2403 54-55 -0.05996325726719931 2403 56-57 -0.02435615445538275 2403 58-59 -0.0593666735698406 2403 60-61 0.013006445918477993 2403 62-63 -0.01997581784032576 2403 64-65 0.15789994106516048 2403 66-67 0.18500638772002986 2403 68-69 0.12169812923126955 2403 70-71 0.06949710742922832 2403 72-73 0.2668655032529941 2403 74-75 0.2661806248715095 2403 76-77 -0.24400281857432304 2403 78-79 0.05438210723794157 2403 80-81 0.026689563705460273 2403 82-83 0.0948485958415688 2403 84-85 -0.1923181014266362 2403 86-87 -0.019964459711829363 2403 88-89 -0.3181955235681002 2403 90-91 -0.6610104917106128 2404 1 -0.4406658586482166 2404 2 -0.3914979326259598 2404 3 -0.3033448866843713 2404 4 -0.2724908290916659 2404 5 -0.12215534938661676 2404 6 -0.017595559050342047 2404 7 0.012687851929442218 2404 8 -0.09033796152930051 2404 9 0.1435182590080828 2404 10-11 0.05441454619302988 2404 12-13 0.09257647636392363 2404 14-15 0.17538791407378795 2404 16-17 0.004745477612459581 2404 18-19 0.03338105885441678 2404 20-21 0.024725879421069408 2404 22-23 0.03899194867733513 2404 24-25 -0.017202311566109074 2404 26-27 0.02060398557269849 2404 28-29 0.0397806652623629 2404 30-31 0.08428246462506195 2404 32-33 0.04283988437344277 2404 34-35 -0.01581084148505596 2404 36-37 0.02040697586537732 2404 38-39 0.04446104968539544 2404 40-41 -0.013718996688993457 2404 42-43 0.05315864597899633 2404 44-45 0.06369508898924892 2404 46-47 0.08212771975411925 2404 48-49 0.06606994588440074 2404 50-51 0.02342376419502301 2404 52-53 -0.016674807177395223 2404 54-55 0.0628216668539352 2404 56-57 0.012979310399188648 2404 58-59 0.11662606596077296 2404 60-61 0.1695348232336329 2404 62-63 0.06921727757114127 2404 64-65 -0.18980822839763078 2404 66-67 0.08513684750626282 2404 68-69 -0.09216220349409099 2404 70-71 -0.08113386073193851 2404 72-73 0.23143761126272722 2404 74-75 0.1835633151739131 2404 76-77 0.04164602277571561 2404 78-79 -0.06203666133575325 2404 80-81 0.026278587511590956 2404 82-83 0.157237516395611 2404 84-85 0.1304218493526932 2404 86-87 -0.602337504623577 2404 88-89 -0.6786337039285755 2404 90-91 -0.3163551470552761 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 14.0 31 352.0 32 2249.0 33 4182.0 34 5687.0 35 6927.0 36 10244.0 37 15558.0 38 42070.0 39 242652.0 40 247469.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.86785590578455 12.526324904745412 29.99047454104607 29.61534464842397 2 26.680545644748964 18.586457258058932 30.43150689676673 24.301490200425377 3 26.70977954585997 19.33394411836529 22.19764841989681 31.75862791587793 4 26.456172801019733 21.61086518278363 21.220497260150605 30.712464756046025 5 26.730157740507515 21.74924316423163 21.753919266233 29.766679829027854 6 27.08554149261176 24.078461527803757 24.136653019376382 24.699343960208104 7 25.563730074609804 23.984073542961255 23.890724691896832 26.561471690532105 8 24.613269045590265 24.989608662219172 24.443197483910744 25.953924808279815 9 24.19830829020928 25.065465428019202 24.95947378265478 25.776752499116736 10-11 24.21086449002778 23.77477468115912 24.81330229787116 27.20105853094194 12-13 24.50528572715118 23.385272703341162 24.64608835408137 27.463353215426288 14-15 24.977485434808212 23.02036355827116 24.54936231823818 27.45278868868245 16-17 25.01524062874521 22.844403571849174 24.25840486037506 27.88195093903056 18-19 25.09274268969387 23.16393720860957 24.11604353277774 27.62727656891882 20-21 25.039054111159604 23.30170902868702 24.14280122756337 27.51643563259001 22-23 24.964842640508206 22.827864025881357 24.305512258314803 27.901781075295634 24-25 24.952632818615736 23.188703230320538 24.314258300946996 27.544405650116726 26-27 25.147816779932246 23.262741512008922 24.324996016653852 27.264445691404976 28-29 25.200119846762405 22.957928937104697 24.258491454856564 27.583459761276334 30-31 25.35120769725693 23.1385320934083 24.251104735794303 27.259155473540464 32-33 25.238620736188583 23.130963298883405 24.423523729790148 27.206892235137865 34-35 25.36758670176364 22.88850930703151 24.46446062381368 27.27944336739117 36-37 25.378919629824114 23.172027729469065 24.42765817022117 27.02139447048565 38-39 25.49534867242304 23.15976475113626 24.522669527983883 26.822217048456814 40-41 25.419444788962497 22.92501528947357 24.594240893675074 27.061299027888857 42-43 25.42324139138263 23.141818545554575 24.63586577217826 26.79907429088454 44-45 25.549508962363298 23.25937697815329 24.523451102176896 26.667662957306515 46-47 25.603240070824356 22.857295704740196 24.62359881159129 26.91586541284416 48-49 25.607679992784504 23.151311190782206 24.66416942884845 26.57683938758484 50-51 25.610157276826495 23.206403074846875 24.604895334941958 26.578544313384672 52-53 25.731126046882284 22.94712503111619 24.559152503579405 26.76259641842212 54-55 25.725544755080463 23.14952132033361 24.562617467402795 26.562316457183133 56-57 25.972171084308098 23.2260988132857 24.42980663799347 26.371923464412735 58-59 25.970384295286657 22.920204496867267 24.55936488423584 26.55004632361024 60-61 25.99115856785812 23.062527989112077 24.325696944527145 26.62061649850266 62-63 26.143686469645516 23.019421936814265 24.291149738313777 26.54574185522644 64-65 26.255071073900453 22.98939412102437 24.026953191543058 26.728581613532114 66-67 26.376742157164205 23.013636916211983 23.97866147982351 26.630959446800297 68-69 26.564438205914996 23.107494940262455 23.98111575373768 26.346951100084876 70-71 26.566972287652852 22.80379883444852 23.82295254272281 26.806276335175816 72-73 26.632322990295915 22.92059204722437 23.65248265571735 26.794602306762368 74-75 26.66760259592743 22.85922134939728 23.602008844531515 26.87116721014377 76-77 26.94040758214878 22.53061409952517 23.164479314183588 27.364499004142463 78-79 26.93929058039266 22.96423640004755 23.031904679170058 27.064568340389734 80-81 27.64116832062358 22.574540703110042 22.67817114793246 27.106119828333913 82-83 27.45683741138806 22.540304139034987 22.4717013491882 27.531157100388747 84-85 28.174723442945897 22.103727837551144 21.74003636914684 27.981512350356113 86-87 28.868999623716608 20.816534967478365 21.55835080363382 28.756114605171206 88-89 28.251917992284685 20.306878765549826 21.178102379610767 30.26310086255472 90-91 24.130121474161005 21.19621165328392 23.6154004529545 31.058266419600578 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.5 5 0.5 6 0.0 7 0.5 8 1.5 9 2.0 10 1.0 11 2.0 12 5.0 13 8.5 14 16.5 15 33.0 16 55.5 17 82.0 18 125.5 19 181.0 20 251.5 21 363.0 22 499.5 23 672.5 24 922.0 25 1200.5 26 1503.0 27 1875.0 28 2422.0 29 3063.0 30 3744.0 31 4611.5 32 5682.5 33 6772.0 34 8053.5 35 9599.5 36 11456.5 37 13412.5 38 15447.0 39 17739.5 40 20234.0 41 23023.0 42 25764.0 43 28279.5 44 30667.5 45 33051.5 46 35231.5 47 37015.0 48 38426.5 49 39788.5 50 40842.0 51 41272.0 52 41438.0 53 41549.0 54 41191.0 55 40302.5 56 39464.0 57 38629.5 58 37335.0 59 35571.0 60 33850.5 61 32015.0 62 30335.0 63 28693.0 64 26550.0 65 24348.0 66 22079.0 67 20002.5 68 17909.5 69 15462.0 70 13220.0 71 11248.5 72 9359.0 73 7576.5 74 5960.5 75 4674.0 76 3608.5 77 2631.5 78 1981.0 79 1507.5 80 1043.5 81 745.0 82 566.5 83 403.0 84 281.0 85 203.5 86 143.0 87 99.5 88 73.0 89 58.0 90 48.0 91 36.0 92 25.0 93 15.0 94 9.5 95 7.0 96 3.5 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.927558520550603E-4 2 0.005542046816440482 3 8.659448150688253E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.575476521633421E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011264051904751177 82-83 0.0014292043619317126 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 9210.0 32-33 20583.0 34-35 22681.0 36-37 24574.0 38-39 25829.0 40-41 27700.0 42-43 28112.0 44-45 28476.0 46-47 28395.0 48-49 28123.0 50-51 27608.0 52-53 27275.0 54-55 25543.0 56-57 24442.0 58-59 23478.0 60-61 21735.0 62-63 20315.0 64-65 19043.0 66-67 17578.0 68-69 16340.0 70-71 15007.0 72-73 13845.0 74-75 12553.0 76-77 11621.0 78-79 10518.0 80-81 9581.0 82-83 8831.0 84-85 7946.0 86-87 7230.0 88-89 6705.0 90-91 6527.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.84750780542677 #Duplication Level Percentage of deduplicated Percentage of total 1 99.85369099504533 99.70142191028461 2 0.14195978617569358 0.2834866171646856 3 0.00279206158331243 0.008363411721990602 4 0.0013023034542567517 0.00520127017255741 5 0.0 0.0 6 2.548537414189731E-4 0.0015267906561343882 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013855117041101205 0.0 0.0 0.0 0.0 2 0.0013855117041101205 0.0 0.0 0.0 0.0 3 0.0013855117041101205 0.0 0.0 0.0 0.0 4 0.0013855117041101205 0.0 0.0 0.0 0.0 5 0.0013855117041101205 0.0 1.7318896301376507E-4 0.0 0.0 6 0.0017318896301376505 0.0 1.7318896301376507E-4 0.0 0.0 7 0.0020782675561651807 0.0 1.7318896301376507E-4 0.0 0.0 8 0.007620314372605663 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 9 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 10-11 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 12-13 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 14-15 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 16-17 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 18-19 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 20-21 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 22-23 0.007966692298633192 0.0 1.7318896301376507E-4 0.0 1.7318896301376507E-4 24-25 0.008053286780140074 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 26-27 0.008313070224660723 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 28-29 0.008659448150688254 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 30-31 0.009092420558222666 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 32-33 0.009525392965757079 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 34-35 0.010477932262332786 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 36-37 0.010737715706853434 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 38-39 0.010824310188360317 0.0 1.7318896301376507E-4 0.0 3.4637792602753014E-4 40-41 0.01099749915137408 0.0 1.7318896301376507E-4 0.0 4.329724075344127E-4 42-43 0.011430471558908493 0.0 1.7318896301376507E-4 0.0 6.061613705481777E-4 44-45 0.011776849484936024 0.0 1.7318896301376507E-4 0.0 6.927558520550603E-4 46-47 0.012123227410963553 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 48-49 0.012296416373977319 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 50-51 0.012729388781511732 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 52-53 0.012815983263018615 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 54-55 0.012902577744525497 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 56-57 0.013248955670553026 0.0 1.7318896301376507E-4 0.0 8.659448150688253E-4 58-59 0.013595333596580557 0.0 1.7318896301376507E-4 0.0 0.0010391337780825904 60-61 0.01368192807808744 0.0 1.7318896301376507E-4 0.0 0.0010391337780825904 62-63 0.01368192807808744 0.0 1.7318896301376507E-4 0.0 0.0010391337780825904 64-65 0.01368192807808744 0.0 1.7318896301376507E-4 0.0 0.0010391337780825904 66-67 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