##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-14A_S87_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1110053 Sequences flagged as poor quality 0 Sequence length 61 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.27500398629615 40.0 40.0 40.0 40.0 40.0 2 39.13851680955774 40.0 40.0 40.0 40.0 40.0 3 39.05790083896895 40.0 40.0 40.0 40.0 40.0 4 39.0180180585972 40.0 40.0 40.0 40.0 40.0 5 39.069752525329875 40.0 40.0 40.0 40.0 40.0 6 39.09046144643544 40.0 40.0 40.0 40.0 40.0 7 39.141932862665115 40.0 40.0 40.0 40.0 40.0 8 38.86147778529494 40.0 40.0 40.0 40.0 40.0 9 39.16809737913415 40.0 40.0 40.0 40.0 40.0 10 39.175271811345944 40.0 40.0 40.0 40.0 40.0 11 39.177347387917514 40.0 40.0 40.0 40.0 40.0 12 39.17415114413456 40.0 40.0 40.0 40.0 40.0 13 39.15605471090119 40.0 40.0 40.0 40.0 40.0 14 39.19025487972196 40.0 40.0 40.0 40.0 40.0 15 39.18322098134053 40.0 40.0 40.0 40.0 40.0 16 39.17994546206352 40.0 40.0 40.0 40.0 40.0 17 39.17799960902768 40.0 40.0 40.0 40.0 40.0 18 39.173549371066066 40.0 40.0 40.0 40.0 40.0 19 39.18946572821298 40.0 40.0 40.0 40.0 40.0 20 38.986578118342095 40.0 40.0 40.0 40.0 40.0 21 39.047295939923586 40.0 40.0 40.0 40.0 40.0 22 39.0586647664571 40.0 40.0 40.0 40.0 40.0 23 39.159081593401396 40.0 40.0 40.0 40.0 40.0 24 39.17749873204252 40.0 40.0 40.0 40.0 40.0 25 39.046986044810474 40.0 40.0 40.0 40.0 40.0 26 39.16840367081572 40.0 40.0 40.0 40.0 40.0 27 39.18047156306951 40.0 40.0 40.0 40.0 40.0 28 39.01052292097765 40.0 40.0 40.0 40.0 40.0 29 39.16502365202382 40.0 40.0 40.0 40.0 40.0 30 38.989630224863134 40.0 40.0 40.0 40.0 40.0 31 39.00078284550377 40.0 40.0 40.0 40.0 40.0 32 38.9772127997492 40.0 40.0 40.0 40.0 40.0 33 39.06832916986846 40.0 40.0 40.0 40.0 40.0 34 38.94950961800923 40.0 40.0 40.0 40.0 40.0 35 39.08103126607468 40.0 40.0 40.0 40.0 40.0 36 38.90532434036933 40.0 40.0 40.0 40.0 40.0 37 38.89386903147868 40.0 40.0 40.0 40.0 40.0 38 38.889804360692686 40.0 40.0 40.0 40.0 40.0 39 38.82624703505148 40.0 40.0 40.0 40.0 40.0 40 38.83090627204287 40.0 40.0 40.0 40.0 40.0 41 38.83508625263839 40.0 40.0 40.0 40.0 40.0 42 38.74807419105214 40.0 40.0 40.0 40.0 40.0 43 38.638962283782845 40.0 40.0 40.0 40.0 40.0 44 38.55185292954481 40.0 40.0 40.0 40.0 40.0 45 38.563960459545626 40.0 40.0 40.0 40.0 40.0 46 38.39553606899851 40.0 40.0 40.0 40.0 40.0 47 38.30503228224238 40.0 40.0 40.0 40.0 40.0 48 38.248804336369524 40.0 40.0 40.0 40.0 40.0 49 38.07316407414781 40.0 40.0 40.0 40.0 40.0 50 38.00773116238594 40.0 40.0 40.0 40.0 40.0 51 37.791618958734404 40.0 40.0 40.0 24.0 40.0 52 37.71513792584678 40.0 40.0 40.0 24.0 40.0 53 37.46539669727481 40.0 40.0 40.0 24.0 40.0 54 37.29141581528089 40.0 40.0 40.0 24.0 40.0 55 37.16969189759408 40.0 40.0 40.0 24.0 40.0 56 36.892070919136295 40.0 40.0 40.0 24.0 40.0 57 36.72329519401326 40.0 40.0 40.0 24.0 40.0 58 36.51431057796339 40.0 40.0 40.0 24.0 40.0 59 36.25135736762119 40.0 40.0 40.0 24.0 40.0 60 35.97279048838209 40.0 40.0 40.0 24.0 40.0 61 35.667170846797404 40.0 40.0 40.0 12.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0373515083243916 1101 2 -0.0011535358327989798 1101 3 0.05963623744494129 1101 4 0.1523186013622393 1101 5 0.08867308669930907 1101 6 0.0694271336554948 1101 7 0.12043492255460819 1101 8 0.3351547690309644 1101 9 0.10816256396400803 1101 10 0.18195659765171257 1101 11 0.07408505309933844 1101 12 0.07756482672543541 1101 13 0.14096147379532908 1101 14 0.10050870805616086 1101 15 0.06707597338334637 1101 16 0.214367226972783 1101 17 0.05908333401387722 1101 18 0.049664551360642406 1101 19 0.03912248815817776 1101 20 0.12228476448768788 1101 21 -0.03602717879270756 1101 22 0.029731869315568815 1101 23 0.05072426612994008 1101 24 -0.02527479881276662 1101 25 0.11003959956509846 1101 26 0.12274595380503683 1101 27 0.13469788831213947 1101 28 0.05033815813555975 1101 29 0.026430900215686393 1101 30 0.09043829057743835 1101 31 0.06650554636967598 1101 32 0.11498818502629149 1101 33 0.1961915325100989 1101 34 0.13720572278861454 1101 35 0.06809570777911489 1101 36 0.05255485845729879 1101 37 0.03954748375408457 1101 38 0.07549067380031005 1101 39 0.08760445948981044 1101 40 0.07442713267533207 1101 41 0.10869064873607925 1101 42 0.11712822470049389 1101 43 0.11436168904380395 1101 44 0.16744692805944794 1101 45 0.07750933161650408 1101 46 0.027820900375424173 1101 47 0.1495326808990214 1101 48 0.14345536204699272 1101 49 0.057079507442026056 1101 50 0.12243225869816143 1101 51 0.06474460410705518 1101 52 0.30252647895390083 1101 53 0.2431961546072472 1101 54 0.11334035007919141 1101 55 0.2577259020626599 1101 56 0.06321550804468501 1101 57 0.03657919058733938 1101 58 0.2224955362288128 1101 59 0.036917447747491394 1101 60 0.10590266379021784 1101 61 0.2206637310685693 1102 1 -0.03588375089146467 1102 2 -0.013328541003751582 1102 3 0.08640645469635189 1102 4 0.10012952934080488 1102 5 0.12583493333551843 1102 6 -0.03990797455839612 1102 7 0.11259932008952234 1102 8 0.3745466124022059 1102 9 0.01755694191690793 1102 10 -0.014359882863317353 1102 11 -0.04454238157923385 1102 12 -0.010861148338690896 1102 13 0.04614760703848475 1102 14 -0.0324584603312843 1102 15 0.050228076099323005 1102 16 -0.034156973620007136 1102 17 0.057828630624420896 1102 18 -0.07157050319347036 1102 19 0.013119638424370805 1102 20 -0.03459356331623553 1102 21 0.010049371167689003 1102 22 0.008099813182433024 1102 23 -0.08165238047400436 1102 24 0.018602212904582416 1102 25 0.015044225060066196 1102 26 -0.009110412027560244 1102 27 -0.018204984685119996 1102 28 0.07105925671741886 1102 29 -0.05565753290780151 1102 30 0.021858820253726208 1102 31 -0.03118975588967743 1102 32 0.04083791867765996 1102 33 0.06353221245242224 1102 34 -0.001882980887081942 1102 35 0.029362140795996083 1102 36 -0.0037439343763168154 1102 37 0.06392297977296124 1102 38 0.022969044774491465 1102 39 0.026669449053891015 1102 40 0.06597676595353619 1102 41 0.11642088968295639 1102 42 -0.039332697442780784 1102 43 0.1586508228058463 1102 44 -0.08398295050907478 1102 45 -0.049216953922226025 1102 46 0.029158898416440593 1102 47 0.1287385440965707 1102 48 0.019384870529293607 1102 49 0.02258433285970085 1102 50 0.004115003356382374 1102 51 0.08887332418061789 1102 52 0.08049650437897071 1102 53 0.055724758006135744 1102 54 0.12620253612556098 1102 55 0.07705141118955794 1102 56 -0.06945857505758113 1102 57 0.07573544684706235 1102 58 0.12744079874450165 1102 59 -0.037089850169351735 1102 60 0.02703568239616061 1102 61 0.013817314751022991 1103 1 -0.1701978170865175 1103 2 -0.15009484101623372 1103 3 -0.11673516160577435 1103 4 -0.1492711490843135 1103 5 -0.22408050050646722 1103 6 -0.069385871919323 1103 7 -0.11597817703818691 1103 8 0.1486914273889326 1103 9 0.02012340873206142 1103 10 -0.11809276337769603 1103 11 -0.07960307529004496 1103 12 0.030605911829319155 1103 13 -0.16407613379174535 1103 14 -0.19234770412972324 1103 15 -0.04212424160143513 1103 16 -0.048311904091313806 1103 17 -0.08720550351275591 1103 18 -0.07352322872691985 1103 19 -0.15596512069261337 1103 20 -0.19349889308087143 1103 21 -0.18400566086012304 1103 22 -0.11917052748093226 1103 23 -0.10209703442594531 1103 24 -0.16116935644633656 1103 25 -0.1973857710791478 1103 26 -0.15177312697156253 1103 27 -0.1055381197575258 1103 28 -0.025854668959794935 1103 29 -0.0199246157617452 1103 30 -0.19716134339669367 1103 31 -6.697836719098405E-4 1103 32 -0.05420971937436292 1103 33 -0.061043932516483324 1103 34 -0.035550385489280245 1103 35 -0.04044402139140857 1103 36 -0.09664604858649994 1103 37 -0.07823171162844034 1103 38 -0.05804737505410884 1103 39 -0.12547540802713542 1103 40 -0.07831252405223665 1103 41 -0.031417495849716204 1103 42 -0.11954064969820877 1103 43 -0.09331214513773745 1103 44 0.006878393606825739 1103 45 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19 -0.027524418090692393 1104 20 -0.0738250416027526 1104 21 -0.07376328961046141 1104 22 0.06351641617523285 1104 23 0.031157555507647317 1104 24 -0.027696554144242214 1104 25 -0.047438096116621864 1104 26 -0.09142725392104012 1104 27 -0.055591661562637285 1104 28 0.009779056261358221 1104 29 0.013493464228879759 1104 30 0.01877671716755458 1104 31 -0.01752886481573057 1104 32 0.04111685340458138 1104 33 -0.0486367704771169 1104 34 -0.08152292892606994 1104 35 -0.02608713022788578 1104 36 0.0814313602367065 1104 37 0.05395985852477736 1104 38 -0.007305424797991122 1104 39 0.0163400689187867 1104 40 -0.13284369824348374 1104 41 0.002058279877928726 1104 42 0.07000531788951747 1104 43 0.004497922913415664 1104 44 0.008953828526720997 1104 45 -0.013414362436428462 1104 46 0.012994819460949714 1104 47 0.04188210234563172 1104 48 0.008422861407687776 1104 49 -0.15851969267413324 1104 50 0.08100958032187577 1104 51 0.05652192170905579 1104 52 0.2027943045797258 1104 53 0.23890445997631815 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0.1803184865496945 2401 4 0.07152123650207898 2401 5 0.07610479262368841 2401 6 0.11621397085323792 2401 7 0.08692363199548936 2401 8 0.40897529537247834 2401 9 0.14404523076380116 2401 10 0.06299671655335004 2401 11 0.07633994068258687 2401 12 0.18114820504211337 2401 13 0.07906855391352252 2401 14 0.0692315035343185 2401 15 0.09896058304753552 2401 16 0.05587608791132226 2401 17 0.052564129506592394 2401 18 0.09388031747638337 2401 19 0.05387494456073938 2401 20 0.01544386407196896 2401 21 0.08395197805823074 2401 22 0.11446904698261307 2401 23 0.16548639525137787 2401 24 0.12003648072152373 2401 25 0.1845965006339867 2401 26 0.07298865584265002 2401 27 0.11483241190992999 2401 28 0.14342091604612506 2401 29 0.08780794175741136 2401 30 0.14665654871838285 2401 31 0.1479935225560638 2401 32 0.1701788467962757 2401 33 0.22820095145011265 2401 34 0.19803360351671273 2401 35 0.06809154746991908 2401 36 0.14686490758014514 2401 37 0.22420280733773978 2401 38 0.0750788031900882 2401 39 0.16844758775110336 2401 40 0.0878482901357529 2401 41 0.026695114828001465 2401 42 0.12802407448005226 2401 43 0.14250618074242993 2401 44 -0.0015656329540902902 2401 45 0.14384130140849294 2401 46 0.08080659827248837 2401 47 -0.03247668603712839 2401 48 0.12470068819963132 2401 49 0.028577229152702444 2401 50 0.016148839714460905 2401 51 0.06989506688945113 2401 52 0.005850670007781389 2401 53 0.07073053696254306 2401 54 0.1049323651977545 2401 55 0.1321885721282925 2401 56 0.09788336455978452 2401 57 -0.060214563124439735 2401 58 -0.006367232852184657 2401 59 -0.06792234394583119 2401 60 -0.06861398628721815 2401 61 0.04442055267554679 2402 1 0.12806764711825025 2402 2 0.21047545008553215 2402 3 0.24527288777399292 2402 4 0.11478039174529187 2402 5 0.09842314739922386 2402 6 0.16531119137820127 2402 7 0.09584465394328845 2402 8 0.3403029787581673 2402 9 0.047201681680284935 2402 10 0.030766883220138652 2402 11 0.008395407240236352 2402 12 0.09750152747844965 2402 13 0.1358573661975555 2402 14 0.18642631909140306 2402 15 0.04683373053941153 2402 16 0.07380898126665159 2402 17 0.1253878714329062 2402 18 0.17223896398730432 2402 19 0.06039240580221161 2402 20 0.1441587861204141 2402 21 0.24240923573272966 2402 22 0.15948001680799706 2402 23 0.17691663977714 2402 24 0.05416963492342575 2402 25 0.10001762861396202 2402 26 0.18578122257935803 2402 27 0.08201995704150278 2402 28 0.07087959744028183 2402 29 0.13634229062970604 2402 30 0.17813489902057 2402 31 0.20212498814300517 2402 32 0.11823270571348843 2402 33 0.19889508822841862 2402 34 0.16092061172303573 2402 35 0.17346902080326032 2402 36 0.20674161400086177 2402 37 0.18265794189985485 2402 38 0.18670513936221766 2402 39 0.1577836386682705 2402 40 0.1841506924374059 2402 41 0.08096087961627774 2402 42 0.16982075092044369 2402 43 0.16210398919671576 2402 44 0.15025566418363212 2402 45 0.08529573707164673 2402 46 0.21403436848359547 2402 47 0.22587251509246897 2402 48 0.17634761856179182 2402 49 0.07594633495455128 2402 50 0.20572499982873182 2402 51 0.0401971684991409 2402 52 0.14534902792848214 2402 53 -0.039498495019181235 2402 54 -0.04757721534683412 2402 55 0.03542896004561413 2402 56 -0.035144931837564286 2402 57 -0.06457237470907984 2402 58 0.20395519138665463 2402 59 0.09969095927881 2402 60 0.11958066954269242 2402 61 0.19206968712460082 2403 1 0.10212339612953514 2403 2 0.10610037168548558 2403 3 0.13083314650490507 2403 4 0.13948612885798184 2403 5 0.09911952185009199 2403 6 0.13500599468386554 2403 7 -0.0030861928301675334 2403 8 0.4023319372116845 2403 9 0.018532832554903678 2403 10 0.14333561461719313 2403 11 0.005073065634803697 2403 12 0.1046330770473034 2403 13 0.09573971068909515 2403 14 0.09295029519093134 2403 15 0.011585400563831172 2403 16 0.09456981007139831 2403 17 -0.029283712219900337 2403 18 0.06892532706251586 2403 19 0.09256232381603269 2403 20 5.54062027347868E-4 2403 21 0.1844886879534613 2403 22 0.05299661842124692 2403 23 0.10155892870435679 2403 24 0.07091902915377801 2403 25 -0.05848864818891997 2403 26 0.2124744732744901 2403 27 0.05417821806475587 2403 28 -0.007357608747788902 2403 29 0.03627784951488877 2403 30 -0.013423679011928868 2403 31 0.10275625952615286 2403 32 0.052985001283396116 2403 33 0.14779728899012667 2403 34 0.013645027822654754 2403 35 0.042660839197800726 2403 36 0.10319033598494798 2403 37 0.04320167663149732 2403 38 0.09839598882320644 2403 39 0.13153522032198595 2403 40 0.1628778430644573 2403 41 0.16066861417184697 2403 42 0.17386925485723737 2403 43 0.14042403879022203 2403 44 -0.022297584135088755 2403 45 0.026599049517059825 2403 46 -0.054015514963047906 2403 47 0.058705908498026815 2403 48 0.11110951244875622 2403 49 0.1709203582678711 2403 50 0.10600576715129506 2403 51 0.019909305041487357 2403 52 0.15437045302702757 2403 53 0.15331382180603725 2403 54 0.06164137242299006 2403 55 -0.09708573613620786 2403 56 0.13925946776618758 2403 57 0.026748330548784338 2403 58 0.17147713429784517 2403 59 0.0894601462318505 2403 60 0.0374644193602407 2403 61 0.14938894998709173 2404 1 -0.029958800703582256 2404 2 0.07992944388846013 2404 3 -0.08700882881733207 2404 4 -0.16073708849958024 2404 5 0.015019423590416636 2404 6 -0.05065662507871593 2404 7 -0.08759088975274665 2404 8 0.3362461127524554 2404 9 -0.09346393065578695 2404 10 -0.004558958531212909 2404 11 -0.09110856888874252 2404 12 -0.048590088015188826 2404 13 -0.020201201391891743 2404 14 0.04531224309953785 2404 15 -0.04410058683581042 2404 16 -0.05642255760144366 2404 17 -0.012404791810240567 2404 18 -0.0417131406370288 2404 19 -0.03470597464016123 2404 20 -0.0252824525891171 2404 21 -0.07396728648250672 2404 22 -0.06838022807622224 2404 23 -0.08532299179062619 2404 24 -0.11911848567462613 2404 25 -0.06659713567801617 2404 26 0.008090220144353566 2404 27 -0.044468165456244435 2404 28 -0.0458247578134916 2404 29 -0.039049063452360144 2404 30 -0.02732590925884182 2404 31 0.02222185900650686 2404 32 0.037834570399581935 2404 33 -0.08933881304579927 2404 34 -0.17176987847284408 2404 35 -0.10339927810828442 2404 36 -0.07454958138885104 2404 37 -0.04395630676068407 2404 38 -0.06558619782613562 2404 39 0.0421753394446398 2404 40 -0.10271916015306459 2404 41 -0.07683217713506707 2404 42 -0.04830220139518815 2404 43 -0.09196559797926085 2404 44 -0.18489119918285724 2404 45 -0.13822811529221468 2404 46 -0.05322283186839627 2404 47 0.032789575097716295 2404 48 -0.11627355991385002 2404 49 -0.0446357722275863 2404 50 -0.07321905483905766 2404 51 -0.24359314319328007 2404 52 -0.08048668511176515 2404 53 -0.10812021897860546 2404 54 -0.09578604065022489 2404 55 -0.07414746908457914 2404 56 -0.10221161994364536 2404 57 -0.16291210933528788 2404 58 -0.12698181269972508 2404 59 -0.24909673015638134 2404 60 -0.10716859313828309 2404 61 -0.10006401610643678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 20.0 14 75.0 15 206.0 16 591.0 17 939.0 18 1168.0 19 1137.0 20 1183.0 21 1186.0 22 1338.0 23 1660.0 24 2278.0 25 3699.0 26 5728.0 27 4256.0 28 3866.0 29 4148.0 30 5269.0 31 6632.0 32 9761.0 33 12940.0 34 17567.0 35 24170.0 36 40934.0 37 54805.0 38 116014.0 39 404289.0 40 384193.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.524328205331475 28.094345384753282 21.72859035952467 19.65273605039057 2 31.576690482346336 17.568170168451417 19.291060877273427 31.56407847192882 3 30.532596191353022 19.13241980337876 18.76162669710365 31.573357308164564 4 29.885960400089008 20.582891087182322 20.630816726768902 28.90033178595977 5 26.79574759043037 21.046922984758385 22.002192688096876 30.155136736714372 6 26.176227621564014 21.08962364860056 23.643375586571093 29.090773143264332 7 27.355360509813497 23.466807440725805 23.755172050343543 25.42265999911716 8 26.11830245943212 25.07853228629624 24.544683902480333 24.25848135179131 9 25.927680930550164 24.82647224952322 24.429914607680896 24.815932212245723 10 27.762638360510717 24.54180115724204 23.327354639823504 24.36820584242374 11 26.519634648075364 24.913495121404114 23.62319636990306 24.943673860617466 12 27.574989662655746 23.520588656577658 23.128445218381465 25.77597646238513 13 27.712640747784118 24.441175331267967 22.38712926319734 25.459054657750574 14 28.06055206373029 24.180286887202683 22.53090618195708 25.22825486710995 15 27.686335697484715 23.588333169677483 23.05412444270679 25.671206690131008 16 27.968664559259782 24.35054902783921 22.037416231477238 25.64337018142377 17 28.24865119052874 24.168845992038218 22.38190428745294 25.200598529980102 18 27.708316629926678 23.519417541324604 22.929265539573336 25.843000289175382 19 28.95195094288291 24.145784030131896 21.785446280492913 25.116818746492285 20 27.718226066683304 24.080832176481664 22.835306061962807 25.36563569487223 21 27.755701754781075 24.228032355211866 22.273621169439657 25.742644720567398 22 28.987805086784146 24.230554757295373 21.872919581317287 24.908720574603194 23 28.393238881386747 23.993088618291196 22.29722364607816 25.316448854243895 24 28.54602437901614 23.466537183359716 22.009759894347386 25.97767854327676 25 28.377293696787454 24.86196605026967 21.78499585154943 24.975744401393445 26 27.84957114660291 24.86358759446621 22.181823750757847 25.105017508173034 27 28.018121657254202 24.460723947415122 21.969221289433925 25.551933105896747 28 28.496116852078234 25.06763190586395 21.766708436444027 24.669542805613787 29 28.056678374816336 25.162131898206663 22.089305645766462 24.691884081210535 30 28.480441924845028 24.56549371966924 21.805355239794856 25.14870911569087 31 28.916276970559064 24.807193890742155 22.079486294798535 24.197042843900245 32 29.219775992677828 24.847191980923434 21.62869700816087 24.304335018237868 33 28.671887457220357 24.43342591182912 22.197488579332987 24.69719805161754 34 29.155634911125865 25.21005753779324 21.17079094421618 24.46351660686472 35 28.74817688885125 25.188617120083457 21.35753878418418 24.705667206881113 36 29.044649219451685 24.644228698990048 21.54852065622092 24.76260142533735 37 29.757317893830294 25.33897030141804 21.38663649393317 23.51707531081849 38 29.15887799951894 25.2296061539404 21.22673421899675 24.384781627543912 39 30.11946276439053 25.03430016404622 20.903686580730827 23.94255049083242 40 30.079374588420553 26.184785771490187 20.588836749236297 23.147002890852956 41 29.796595297701998 26.221450687489696 20.778737591808678 23.203216422999624 42 30.139551895269868 25.65490116237693 20.677481165313726 23.528065777039476 43 30.724118578121946 25.491395455892647 20.941522611983395 22.842963354002016 44 30.908524187583836 25.584003646672727 20.692075063082573 22.815397102660864 45 30.93005469108232 25.10717956710175 20.92837008683369 23.03439565498224 46 31.315531780914966 25.854441184339848 20.074536981567547 22.755490053177642 47 31.161845425398603 26.281627994339008 20.26236585099991 22.294160729262476 48 31.462551788067778 26.175867278409232 20.08390590359199 22.277675029931004 49 31.853282548925637 26.11120920461384 19.878167869613314 22.157340376847213 50 31.581825372302042 26.115509799982522 20.038863009243702 22.26380181847173 51 31.965050317417276 25.870026025784355 19.950128507377578 22.214795149420794 52 32.16281759773416 26.43822091217348 19.68196084507753 21.717000645014828 53 32.00865183914642 26.43495400670058 19.796982666593397 21.759411487559603 54 32.71005978993796 26.66233053737074 19.738426903940624 20.88918276875068 55 32.31638489333392 26.918714692001195 20.335245254055437 20.429655160609446 56 31.85144813296698 27.090221161208955 20.555020044142154 20.503310661681905 57 32.771557342860824 26.99785865694459 19.615585229867815 20.61499877032677 58 31.923881111982944 27.52598299360481 20.435060307931245 20.11507558648101 59 31.5775913402333 27.809573056421634 19.90868904457715 20.704146558767913 60 31.48626772331672 27.75715306261255 20.368199962344004 20.388379251726725 61 31.46221978790183 28.089765164154475 19.680429385290058 20.767585662653644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 1.0 2 2.5 3 5.0 4 4.0 5 3.0 6 3.0 7 4.0 8 5.0 9 6.5 10 8.0 11 9.5 12 11.5 13 12.0 14 35.0 15 58.0 16 49.0 17 50.0 18 60.0 19 88.0 20 153.0 21 190.0 22 221.0 23 252.0 24 430.5 25 794.5 26 980.0 27 1232.5 28 1485.0 29 2002.0 30 3125.5 31 3732.0 32 4769.5 33 5807.0 34 7005.5 35 9949.5 36 11695.0 37 13788.5 38 15882.0 39 18951.0 40 25441.5 41 28863.0 42 32574.5 43 40333.0 44 44380.0 45 49270.0 46 54160.0 47 58483.5 48 66761.5 49 70716.0 50 74567.5 51 78419.0 52 80416.0 53 83518.0 54 84623.0 55 83294.0 56 81965.0 57 78974.5 58 71709.5 59 67435.0 60 62956.0 61 54657.5 62 50838.0 63 46451.0 64 42064.0 65 37907.0 66 29825.0 67 25900.0 68 22315.0 69 18730.0 70 16166.5 71 11479.5 72 9356.0 73 7572.5 74 5789.0 75 4652.5 76 2886.5 77 2257.0 78 1715.0 79 1173.0 80 893.5 81 499.0 82 384.0 83 283.5 84 136.5 85 90.0 86 70.0 87 50.0 88 44.0 89 33.0 90 28.0 91 30.0 92 32.0 93 32.5 94 39.0 95 45.0 96 54.5 97 64.0 98 99.5 99 645.0 100 1155.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.6034315478630297E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 3.6034315478630297E-4 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 9.008578869657574E-5 50 0.0 51 0.0 52 9.008578869657574E-5 53 0.0 54 0.0 55 0.0 56 2.7025736608972726E-4 57 1.8017157739315149E-4 58 0.0 59 0.0 60 3.6034315478630297E-4 61 9.008578869657574E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 61 1110053.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.63264472728679 #Duplication Level Percentage of deduplicated Percentage of total 1 76.79161064274429 55.775777738505816 2 16.217263266257778 23.55805442533959 3 4.463991799355175 9.726945912842583 4 1.3880985623785869 4.032850789108058 5 0.4740969017119288 1.7217455914174964 6 0.2021744637064707 0.8810679597193092 7 0.10543004664258451 0.536036418496048 8 0.07172688129453905 0.4167770469170022 9 0.05514620897217658 0.36048735038995344 >10 0.22708994228127738 2.68045316359541 >50 0.002697027724163484 0.11949427647037357 >100 5.394055448326967E-4 0.07718154906447079 >500 0.0 0.0 >1k 1.3485138620817418E-4 0.11312777813383727 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1155 0.104049085944545 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.7082544707324785 0.0 0.0 0.0 0.0 2 0.7104165296611964 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 3 0.711767816491645 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 4 0.7132091891107902 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 5 0.7142001327864526 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 6 0.7171729638134395 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 7 0.720686309572606 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 8 0.7326677194692506 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 9 0.7377125236362588 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 10 0.7432077567467499 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 11 0.7523064214051041 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 12 0.7642878313017487 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 13 0.78608859216632 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 14 0.8167177603231558 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 15 0.8568960220818286 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 16 0.9112177526658637 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 17 0.9677916279673132 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 18 1.0646338508161322 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 19 1.1924655849765733 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 20 1.366241071372268 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 21 1.5758707016691995 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 22 1.8317143415674748 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 23 2.1508882909194424 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 24 2.5460045601426238 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 25 2.9885960400089004 9.008578869657574E-5 0.0 0.0 9.008578869657574E-5 26 3.504066922930707 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 27 4.100344758313342 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 28 4.750403809547833 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 29 5.491719764731954 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 30 6.313302157644725 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 31 7.199205803686851 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 32 8.149700960224422 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 33 9.192714221753375 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 34 10.272032056127049 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 35 11.40062681691775 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 36 12.58768725457253 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 37 13.836906886427945 1.8017157739315149E-4 0.0 0.0 9.008578869657574E-5 38 15.132700871039491 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 39 16.511553952829278 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 40 17.91752285701674 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 41 19.35051749781317 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 42 20.8176546525256 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 43 22.28929609667286 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 44 23.80652094990059 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 45 25.303566586460285 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 46 26.84583528894566 2.7025736608972726E-4 0.0 0.0 1.8017157739315149E-4 47 28.38359970199621 2.7025736608972726E-4 2.7025736608972726E-4 0.0 1.8017157739315149E-4 48 29.94109290277131 2.7025736608972726E-4 2.7025736608972726E-4 0.0 1.8017157739315149E-4 49 31.508675711880425 2.7025736608972726E-4 2.7025736608972726E-4 0.0 1.8017157739315149E-4 50 33.022387219348985 2.7025736608972726E-4 2.7025736608972726E-4 0.0 1.8017157739315149E-4 >>END_MODULE