##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-14A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 487661 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26175355421081 40.0 40.0 40.0 40.0 40.0 2 39.152907450052396 40.0 40.0 40.0 40.0 40.0 3 39.13314782195008 40.0 40.0 40.0 40.0 40.0 4 39.0722325549921 40.0 40.0 40.0 40.0 40.0 5 39.186426636536446 40.0 40.0 40.0 40.0 40.0 6 39.24445875310923 40.0 40.0 40.0 40.0 40.0 7 39.29605197052871 40.0 40.0 40.0 40.0 40.0 8 39.28957205927888 40.0 40.0 40.0 40.0 40.0 9 39.3091266268986 40.0 40.0 40.0 40.0 40.0 10-11 39.30411904991377 40.0 40.0 40.0 40.0 40.0 12-13 39.30129331646369 40.0 40.0 40.0 40.0 40.0 14-15 39.31595924217848 40.0 40.0 40.0 40.0 40.0 16-17 39.45559312719287 40.0 40.0 40.0 40.0 40.0 18-19 39.470213939601486 40.0 40.0 40.0 40.0 40.0 20-21 39.48132821775783 40.0 40.0 40.0 40.0 40.0 22-23 39.48216896573644 40.0 40.0 40.0 40.0 40.0 24-25 39.473470300065 40.0 40.0 40.0 40.0 40.0 26-27 39.45149601875073 40.0 40.0 40.0 40.0 40.0 28-29 39.445487746610866 40.0 40.0 40.0 40.0 40.0 30-31 39.44080538213066 40.0 40.0 40.0 40.0 40.0 32-33 39.44614490257294 40.0 40.0 40.0 40.0 40.0 34-35 39.43533535550736 40.0 40.0 40.0 40.0 40.0 36-37 39.43095954717151 40.0 40.0 40.0 40.0 40.0 38-39 39.42654783217853 40.0 40.0 40.0 40.0 40.0 40-41 39.409679427056744 40.0 40.0 40.0 40.0 40.0 42-43 39.408727651338175 40.0 40.0 40.0 40.0 40.0 44-45 39.40147560800095 40.0 40.0 40.0 40.0 40.0 46-47 39.39051402590108 40.0 40.0 40.0 40.0 40.0 48-49 39.3832316685225 40.0 40.0 40.0 40.0 40.0 50-51 39.380438961209464 40.0 40.0 40.0 40.0 40.0 52-53 39.37145484013122 40.0 40.0 40.0 40.0 40.0 54-55 39.352722830691725 40.0 40.0 40.0 40.0 40.0 56-57 39.348154780001295 40.0 40.0 40.0 40.0 40.0 58-59 39.33985077508475 40.0 40.0 40.0 40.0 40.0 60-61 39.31159562452743 40.0 40.0 40.0 40.0 40.0 62-63 39.36686459943823 40.0 40.0 40.0 40.0 40.0 64-65 39.36415247357344 40.0 40.0 40.0 40.0 40.0 66-67 39.38255536336024 40.0 40.0 40.0 40.0 40.0 68-69 39.36408718881388 40.0 40.0 40.0 40.0 40.0 70-71 39.35696885358453 40.0 40.0 40.0 40.0 40.0 72-73 39.37050652089718 40.0 40.0 40.0 40.0 40.0 74-75 39.36039804339553 40.0 40.0 40.0 40.0 40.0 76-77 39.35113225907938 40.0 40.0 40.0 40.0 40.0 78-79 39.321661019084075 40.0 40.0 40.0 40.0 40.0 80-81 39.32125196673046 40.0 40.0 40.0 40.0 40.0 82-83 39.322280181285606 40.0 40.0 40.0 40.0 40.0 84-85 39.304875944188225 40.0 40.0 40.0 40.0 40.0 86-87 39.29198609371457 40.0 40.0 40.0 40.0 40.0 88-89 39.34735035757821 40.0 40.0 40.0 40.0 40.0 90-91 39.44623349982405 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20365122929923984 1101 2 0.10421712765493396 1101 3 0.07257459212915762 1101 4 -0.14507595706061238 1101 5 0.12748431298983576 1101 6 -0.03132059843782997 1101 7 0.02688315338535574 1101 8 -0.21026194831834033 1101 9 0.06715169440504098 1101 10-11 0.024520417643586256 1101 12-13 0.01333126543797647 1101 14-15 -0.013211619580360434 1101 16-17 0.004068132969607063 1101 18-19 0.04695873183442956 1101 20-21 0.06153616792455807 1101 22-23 -0.011446830334442382 1101 24-25 0.039541938454036085 1101 26-27 -0.01785741773256433 1101 28-29 0.059864366417237136 1101 30-31 0.09730075345220968 1101 32-33 0.04241471201206082 1101 34-35 0.11857532255115188 1101 36-37 0.11937398722261605 1101 38-39 0.02210276773925557 1101 40-41 0.10863280702285749 1101 42-43 0.13303543850293664 1101 44-45 -0.006571119309008111 1101 46-47 0.11379905263662948 1101 48-49 0.013686436969038596 1101 50-51 0.04347105279287433 1101 52-53 0.14732328882837464 1101 54-55 -0.04769940093868286 1101 56-57 0.16850853827718026 1101 58-59 0.15873689786237577 1101 60-61 0.05872612059745563 1101 62-63 -0.1299825327165749 1101 64-65 0.10970750633477877 1101 66-67 -0.2525359395661724 1101 68-69 -0.08440137724264929 1101 70-71 -0.12700591407864437 1101 72-73 0.03958051265259144 1101 74-75 -0.14684876106287703 1101 76-77 -0.11992801958275123 1101 78-79 -0.22691160563205415 1101 80-81 -0.11904444510867052 1101 82-83 0.09766395681830886 1101 84-85 0.14461133224006772 1101 86-87 -0.8158117633640813 1101 88-89 -0.6254919937957624 1101 90-91 0.8658920027662589 1102 1 0.35967491493701687 1102 2 0.15608306534250005 1102 3 0.13572689499429913 1102 4 0.09877244714142819 1102 5 -0.08927147283038295 1102 6 -5.301596481075421E-4 1102 7 -0.036395278988088364 1102 8 0.06860891064587094 1102 9 0.04715200601912528 1102 10-11 0.008975326715344067 1102 12-13 -0.020645056343617796 1102 14-15 -0.14315914228551918 1102 16-17 0.04763474901135112 1102 18-19 -0.059044958566595085 1102 20-21 -0.0516850981040875 1102 22-23 0.006470979226328666 1102 24-25 0.11302053835713366 1102 26-27 0.01755529656703203 1102 28-29 0.025594237075132753 1102 30-31 -0.06571792632797013 1102 32-33 0.02396879109171124 1102 34-35 0.019660885164540787 1102 36-37 -0.058012922016587254 1102 38-39 0.050144889590015396 1102 40-41 0.031472376971962035 1102 42-43 0.1289674367496687 1102 44-45 -0.02974402109187224 1102 46-47 -0.05740228504166822 1102 48-49 -0.10935742836896623 1102 50-51 -0.11402988394295477 1102 52-53 0.012438868649951473 1102 54-55 0.020009750789895975 1102 56-57 -0.04714432943676883 1102 58-59 -0.04219336789631001 1102 60-61 0.14535486472908588 1102 62-63 -0.13608386559543106 1102 64-65 -0.1405294604969498 1102 66-67 -0.13160466615758537 1102 68-69 -0.056797709071737756 1102 70-71 -0.20668675641056922 1102 72-73 -0.17594647062404078 1102 74-75 0.06919570246063955 1102 76-77 0.048756315313816856 1102 78-79 0.22225985782512225 1102 80-81 0.4372263296950365 1102 82-83 0.128002960106393 1102 84-85 0.021027669525970794 1102 86-87 -0.1259566909003098 1102 88-89 0.17906641076264407 1102 90-91 0.8658920027662589 1103 1 0.20732183940496185 1103 2 0.1373902664853972 1103 3 0.14101074941480363 1103 4 0.02594487217165664 1103 5 -0.018762858198243748 1103 6 0.06617850241070045 1103 7 -0.01742170262460263 1103 8 0.07367454420649011 1103 9 -0.009111150880222851 1103 10-11 -0.006257944109833602 1103 12-13 -0.03407436806048025 1103 14-15 0.013965696049190512 1103 16-17 0.05404171345735165 1103 18-19 0.0617373777641248 1103 20-21 0.09461162116350863 1103 22-23 0.023160510589754324 1103 24-25 0.1584090224059196 1103 26-27 0.11603400621253712 1103 28-29 0.12745391524300942 1103 30-31 0.16414137940964935 1103 32-33 0.1181339116448541 1103 34-35 0.051325402530295605 1103 36-37 -0.029413924075598175 1103 38-39 0.042096447257961245 1103 40-41 -0.002535706363794077 1103 42-43 -0.015376416489282008 1103 44-45 0.12608281489440998 1103 46-47 0.007670068731080448 1103 48-49 -0.007363293810364269 1103 50-51 0.010865247044172577 1103 52-53 0.04920002639784826 1103 54-55 4.5930768749258277E-4 1103 56-57 0.13238012780797703 1103 58-59 0.09405579624168325 1103 60-61 0.06437535202126554 1103 62-63 -0.03284899381040418 1103 64-65 -0.154161664039961 1103 66-67 -0.05162451918673838 1103 68-69 0.009440948950334871 1103 70-71 -0.10520780005553831 1103 72-73 -0.27937716915813837 1103 74-75 -0.019926568400215672 1103 76-77 -0.13055705390169692 1103 78-79 -0.16143408719104713 1103 80-81 0.24858674580121232 1103 82-83 0.25837045909885603 1103 84-85 -0.03511268135122236 1103 86-87 0.07544191049829152 1103 88-89 -0.03925717598199441 1103 90-91 -1.4198222829480258 1104 1 0.1510829213505147 1104 2 0.10464124864998325 1104 3 0.22361131263838985 1104 4 -0.021221278786171638 1104 5 -0.012546538621009518 1104 6 -0.03833632566703926 1104 7 0.15376767261441415 1104 8 -0.1509445845352957 1104 9 0.007190347572652911 1104 10-11 -0.054624248041974965 1104 12-13 -0.10700278303806243 1104 14-15 -0.13796560458008855 1104 16-17 0.001424114458643544 1104 18-19 -0.10282264726803447 1104 20-21 -0.07654854988957993 1104 22-23 -0.14480806014348246 1104 24-25 -0.09012277191317253 1104 26-27 -0.14974911794001855 1104 28-29 -0.17154966574646124 1104 30-31 -0.08744294767723915 1104 32-33 0.013552960287448457 1104 34-35 -0.05226592354573256 1104 36-37 0.03957652293476599 1104 38-39 -0.09370659871127884 1104 40-41 -0.04817854346553929 1104 42-43 -0.017705232607944765 1104 44-45 -0.11803123609243471 1104 46-47 -0.13495479913316188 1104 48-49 -0.0869957128083314 1104 50-51 -0.01751545348596295 1104 52-53 -0.04129760898359791 1104 54-55 -0.10031539041671067 1104 56-57 -0.20661233882532315 1104 58-59 0.06634696672129081 1104 60-61 -0.03891117204123162 1104 62-63 -0.05940794080374445 1104 64-65 -0.114007109270446 1104 66-67 -0.2165239410955735 1104 68-69 -0.40554410245808015 1104 70-71 -0.09002593735279873 1104 72-73 -0.02667861792150461 1104 74-75 -0.11429765016625737 1104 76-77 -0.3806212214126745 1104 78-79 -0.6014213050348829 1104 80-81 -0.5182425864826641 1104 82-83 -0.9803199123146271 1104 84-85 -0.29089658019317 1104 86-87 0.22605619154735024 1104 88-89 0.4309182626144974 1104 90-91 0.8658920027662589 1201 1 0.23309089695455043 1201 2 0.19400554609310205 1201 3 0.0721123682330358 1201 4 0.15720705806580781 1201 5 -0.00757351492877234 1201 6 0.14420179302872072 1201 7 -0.007133101488328464 1201 8 0.03782388837110773 1201 9 -0.013189666501943975 1201 10-11 -0.01635073173889623 1201 12-13 -0.03296102840517534 1201 14-15 -0.08190179974781131 1201 16-17 -0.005040816341264076 1201 18-19 -0.02770184032457479 1201 20-21 0.015189662636799994 1201 22-23 -0.10603724147118498 1201 24-25 -0.03628275314704865 1201 26-27 0.016969155576283868 1201 28-29 0.04431709475084489 1201 30-31 -0.012303374606631223 1201 32-33 0.05498307087025722 1201 34-35 0.06411024326412473 1201 36-37 0.04739399630101104 1201 38-39 -0.030690964336784532 1201 40-41 0.0012473391477669793 1201 42-43 0.028527334754116396 1201 44-45 0.054447437992735104 1201 46-47 -0.2059971804843883 1201 48-49 0.01945699388727462 1201 50-51 0.13101622266429302 1201 52-53 0.04688732629337977 1201 54-55 -0.08170534020639764 1201 56-57 -0.04593565123266785 1201 58-59 0.002412131303692888 1201 60-61 0.13636086742751274 1201 62-63 -0.00448590704724694 1201 64-65 -0.06880727568031375 1201 66-67 0.01188385983296314 1201 68-69 -0.11985721219217993 1201 70-71 0.2084385797780257 1201 72-73 0.0768626874791778 1201 74-75 -0.09353273325971401 1201 76-77 0.007590949065701125 1201 78-79 -0.18824004416413942 1201 80-81 -0.2108709402271316 1201 82-83 -0.22835292864704115 1201 84-85 -0.5561452892097591 1201 86-87 0.2212960563524362 1201 88-89 0.1195204131521308 1201 90-91 0.8658920027662589 1202 1 0.19221422917911468 1202 2 0.24289703971238907 1202 3 0.09843774481897327 1202 4 0.30202302858307206 1202 5 0.01328192781380011 1202 6 0.1540962486488624 1202 7 -0.011188033140555831 1202 8 -0.03478473152657102 1202 9 0.011282479602314766 1202 10-11 0.0696608100327083 1202 12-13 0.058422769819735265 1202 14-15 0.06742845014791499 1202 16-17 -0.04639267141969583 1202 18-19 -0.00939656944141376 1202 20-21 -0.04152394197568299 1202 22-23 0.037640919448357124 1202 24-25 0.01599049011189635 1202 26-27 -0.003928526037661584 1202 28-29 0.11122610694204127 1202 30-31 -0.11303780298270993 1202 32-33 0.03201105197646115 1202 34-35 7.0405202809809E-4 1202 36-37 -0.04139749335166698 1202 38-39 0.10917102380210508 1202 40-41 0.10905841975609576 1202 42-43 0.08602138315404062 1202 44-45 0.03377320912247228 1202 46-47 0.005574195736080867 1202 48-49 0.1035332621790701 1202 50-51 -0.016938854419706217 1202 52-53 -0.04418137579479264 1202 54-55 0.006516502385608192 1202 56-57 0.13558734460723088 1202 58-59 -0.03984992946637789 1202 60-61 0.06502038263087684 1202 62-63 0.02404982549943213 1202 64-65 -0.13809241501164848 1202 66-67 0.06852861652795639 1202 68-69 0.026363972695087057 1202 70-71 0.11921668585596734 1202 72-73 0.17518555684366532 1202 74-75 0.03152384616468851 1202 76-77 0.21632742315589581 1202 78-79 0.37228404747195754 1202 80-81 -0.29308382406608047 1202 82-83 0.35557361190841164 1202 84-85 0.4426726911709906 1202 86-87 -0.4298782595277615 1202 88-89 0.3291638766495879 1202 90-91 0.06589200276626173 1203 1 0.1655794165058211 1203 2 0.21967074163212175 1203 3 0.20080645650858742 1203 4 0.2340895821474689 1203 5 0.064615599252555 1203 6 0.07970618997813261 1203 7 0.04619034544656131 1203 8 0.10273493050530647 1203 9 -0.04362189405738803 1203 10-11 0.16831851433119027 1203 12-13 0.005042444981093297 1203 14-15 -0.1062667761295728 1203 16-17 0.1209463444875638 1203 18-19 -0.0055941706091644505 1203 20-21 0.037635205277808836 1203 22-23 0.09728863762289564 1203 24-25 -0.018734395906250256 1203 26-27 -0.022505445451010075 1203 28-29 -0.06794874419288277 1203 30-31 -0.0335118027613035 1203 32-33 -0.03258506803171457 1203 34-35 -0.002766817963973267 1203 36-37 0.0890771747862118 1203 38-39 -0.03305863857370639 1203 40-41 0.02378646361486858 1203 42-43 0.04811076279711557 1203 44-45 0.00991032649077539 1203 46-47 0.06462683625270671 1203 48-49 0.1417731623292866 1203 50-51 -0.2601715481398159 1203 52-53 -0.04043673329179853 1203 54-55 -0.004023497561675526 1203 56-57 0.13428206155231237 1203 58-59 -0.06926996615262482 1203 60-61 -0.13780516216980487 1203 62-63 0.08782105352621272 1203 64-65 -0.045395142162348634 1203 66-67 0.16247767310431271 1203 68-69 -0.13827592719591308 1203 70-71 -0.13357130397103134 1203 72-73 -0.05164529485227831 1203 74-75 -0.2045017317989064 1203 76-77 0.07672701092167955 1203 78-79 -0.08807425250898859 1203 80-81 0.028801799564654118 1203 82-83 0.2840263396092695 1203 84-85 0.6602433558004819 1203 86-87 0.06945491503761048 1203 88-89 0.20367188580290474 1203 90-91 -5.634107997233741 1204 1 0.2653560864197715 1204 2 0.1676395701922857 1204 3 -0.015330788414175345 1204 4 -0.008566648909628327 1204 5 -0.12897816892108693 1204 6 -0.16013149108139402 1204 7 -0.03133577998875836 1204 8 -0.035639873873165584 1204 9 -0.20467556404948795 1204 10-11 -0.05274688525980764 1204 12-13 -0.1845142734143792 1204 14-15 -0.1840526020231863 1204 16-17 -0.09230096358179907 1204 18-19 -0.022201461053349192 1204 20-21 -0.12244981892909834 1204 22-23 0.011891495927990547 1204 24-25 -0.09625682891798704 1204 26-27 -0.10086113538342545 1204 28-29 -0.0984580094067482 1204 30-31 -0.14229306297315958 1204 32-33 -0.1185324571899784 1204 34-35 -0.004006678239761641 1204 36-37 -0.1893198456438654 1204 38-39 -0.23383256218460247 1204 40-41 -0.09014762700410728 1204 42-43 -0.2708975769161768 1204 44-45 -0.13741585526458522 1204 46-47 -0.03238677441569138 1204 48-49 -0.06684424723083993 1204 50-51 -0.01590405299330655 1204 52-53 -0.06108241991400831 1204 54-55 0.031124983020106356 1204 56-57 -0.1228270731992751 1204 58-59 0.058544610113536066 1204 60-61 0.015986508679475264 1204 62-63 -0.07095887047526617 1204 64-65 0.08059519010959804 1204 66-67 -0.0352679341968809 1204 68-69 -0.0224258758841529 1204 70-71 -0.03370249915767687 1204 72-73 -0.09227627417010353 1204 74-75 0.293078125734354 1204 76-77 0.04076622718378786 1204 78-79 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0.13346912861045723 2401 58-59 0.006907585516181314 2401 60-61 -0.015836024989063446 2401 62-63 0.10641020488034769 2401 64-65 0.13624652185566077 2401 66-67 0.22738147395448038 2401 68-69 0.13389609807980918 2401 70-71 0.2697786433688165 2401 72-73 0.20323652803482162 2401 74-75 0.2870510905160515 2401 76-77 0.06323063038616539 2401 78-79 0.29377608612397665 2401 80-81 0.19824770387550927 2401 82-83 0.08720749112701043 2401 84-85 0.06105900520947927 2401 86-87 0.4914346134475167 2401 88-89 0.46901350070973535 2401 90-91 0.5180659158097356 2402 1 0.2795562833812113 2402 2 0.25826671704675164 2402 3 0.24899877870220166 2402 4 0.4689198338045344 2402 5 0.19505723126327723 2402 6 0.12806050317072248 2402 7 0.15339274874501996 2402 8 0.2178797524832774 2402 9 0.12358521507959352 2402 10-11 0.16503819660205465 2402 12-13 0.162560872469669 2402 14-15 0.1624276938621989 2402 16-17 0.03187883352380538 2402 18-19 0.013381455010197385 2402 20-21 0.1024866818055159 2402 22-23 0.036882194106929944 2402 24-25 0.059164645633515534 2402 26-27 0.017367828990501266 2402 28-29 0.008077681398781067 2402 30-31 0.1489151706839138 2402 32-33 0.0651989293277353 2402 34-35 0.14380587854510907 2402 36-37 0.0368659970051155 2402 38-39 0.04542870667224008 2402 40-41 0.08609952253537756 2402 42-43 0.09063403718548813 2402 44-45 -0.03656052398329024 2402 46-47 -0.05853816995290373 2402 48-49 0.0647579302165866 2402 50-51 0.09751495480178818 2402 52-53 0.008834654275574394 2402 54-55 0.13169434409689273 2402 56-57 0.14211099272377226 2402 58-59 0.012468752812942796 2402 60-61 0.055547471772165125 2402 62-63 0.16736057032956353 2402 64-65 0.13598882069082663 2402 66-67 0.20651103385327474 2402 68-69 0.18286273585208335 2402 70-71 0.17528800342383022 2402 72-73 0.25382271765798237 2402 74-75 0.23238646338715796 2402 76-77 0.10286282379944822 2402 78-79 0.18616817173343492 2402 80-81 -0.0037209465064762526 2402 82-83 0.06742542828587261 2402 84-85 0.06329400534070118 2402 86-87 -0.2996656110881091 2402 88-89 -0.8954886637924346 2402 90-91 -0.9383038014295408 2403 1 -0.3147334051800996 2403 2 -0.10546079216634041 2403 3 0.0010943058356644997 2403 4 -0.03997411325329381 2403 5 0.05667114845382315 2403 6 -0.01623881999810095 2403 7 -0.03402144303183974 2403 8 0.08667446308375304 2403 9 0.12672549496494412 2403 10-11 0.1697124866519033 2403 12-13 0.19326365687123825 2403 14-15 0.17836092603215548 2403 16-17 0.036044295864542164 2403 18-19 0.0017521826639352867 2403 20-21 0.06417707369318038 2403 22-23 0.058474252781692826 2403 24-25 0.0684996770664199 2403 26-27 0.09523954419150726 2403 28-29 0.09203876962769186 2403 30-31 -0.002998503232795713 2403 32-33 -0.001973045219415326 2403 34-35 -0.01960765978002854 2403 36-37 0.059233602870953916 2403 38-39 0.0231639292918544 2403 40-41 0.1314787251493641 2403 42-43 -0.032429533649924736 2403 44-45 0.0517832011562831 2403 46-47 0.1389257753476585 2403 48-49 0.034322229510848956 2403 50-51 0.09685087130669245 2403 52-53 -0.04596311681761733 2403 54-55 0.04745113215543739 2403 56-57 -0.02986663299867587 2403 58-59 0.1927682282047627 2403 60-61 0.10612082725718608 2403 62-63 0.12426704872182626 2403 64-65 0.06195127443850623 2403 66-67 0.20317364866150456 2403 68-69 0.21057424001353553 2403 70-71 0.19730865126499708 2403 72-73 0.20791699024084664 2403 74-75 0.3957904438663107 2403 76-77 0.15995913974658293 2403 78-79 -0.10127590465801006 2403 80-81 0.09196633778903873 2403 82-83 0.15842487094316482 2403 84-85 0.13027136700496555 2403 86-87 0.19487664243302305 2403 88-89 -0.14087660918037415 2403 90-91 0.5900299338007429 2404 1 -0.45937360104980485 2404 2 -0.23007047210388976 2404 3 -0.39563055400395086 2404 4 -0.3434597896897884 2404 5 -0.05576764226365327 2404 6 -0.2101598807224434 2404 7 0.07811573672829297 2404 8 0.038044131666538306 2404 9 0.06626432589721531 2404 10-11 0.18281038602029298 2404 12-13 0.09113138568158519 2404 14-15 0.1825834794350385 2404 16-17 0.13561878785706227 2404 18-19 0.1162002346119877 2404 20-21 0.018769839761361595 2404 22-23 0.0378893049426452 2404 24-25 0.02407859268632251 2404 26-27 0.061766641866135785 2404 28-29 0.029751671479935737 2404 30-31 0.06274085758852976 2404 32-33 -0.01134131288098672 2404 34-35 0.08627600185327822 2404 36-37 0.09175183068227 2404 38-39 0.0827235879730921 2404 40-41 0.021654306220824537 2404 42-43 0.11813705383287498 2404 44-45 0.041288857229588416 2404 46-47 0.0355721070319035 2404 48-49 0.017923886576490133 2404 50-51 0.003912928594260734 2404 52-53 0.09683940338102559 2404 54-55 0.014304943404347625 2404 56-57 0.05647561720870442 2404 58-59 0.08666193549198198 2404 60-61 -0.10433253107286333 2404 62-63 0.17224863482053365 2404 64-65 0.005899866190112846 2404 66-67 -0.04215571410924923 2404 68-69 0.14233417891318112 2404 70-71 0.1704359968873277 2404 72-73 0.22905412377816958 2404 74-75 0.05797642742343356 2404 76-77 0.07848972083656491 2404 78-79 0.08964201508255343 2404 80-81 0.15919390007961454 2404 82-83 -0.12105109769665034 2404 84-85 0.09231789133166046 2404 86-87 -0.10291747521403494 2404 88-89 0.06179304820626186 2404 90-91 0.8658920027662589 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 287.0 32 1977.0 33 3702.0 34 4956.0 35 5970.0 36 8747.0 37 13891.0 38 36570.0 39 209661.0 40 201887.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 28.027133881260564 12.385985202683859 29.14636547074167 30.44051544531391 2 26.79319962265699 18.480168984044955 29.662647143267296 25.063984250030764 3 26.526992277374717 19.178399616124413 21.718909563669623 32.57569854283125 4 26.518421608453412 21.26108095582792 20.70618728994117 31.514310145777497 5 26.89491265448744 21.42287367659091 21.174545432175222 30.507668236746426 6 26.990675899856665 24.01340275314204 23.871911020155395 25.1240103268459 7 25.56694096923888 23.632195316008456 23.55017112297272 27.250692591779945 8 24.431726137624292 24.875066900982446 24.241224949298797 26.451982012094465 9 24.26849799348318 24.77725305078733 24.793657889394478 26.160591066335016 10-11 24.256707015734293 23.54514714114928 24.534563969642846 27.663581873473582 12-13 24.542663858705126 23.166502960048067 24.517236358864047 27.773596822382764 14-15 24.9528873541251 22.79154986763346 24.44351711537318 27.81204566286826 16-17 25.03255335161106 22.646161985477615 24.165865221947215 28.155419440964113 18-19 25.130469732047466 22.93960353606296 23.973108368313234 27.956818363576335 20-21 25.12903430866934 22.928940390968318 23.995972612121946 27.946052688240396 22-23 25.016968754934265 22.59356397169345 24.20964563498004 28.179821638392244 24-25 25.06033904700191 22.83594546211405 24.221949263935397 27.88176622694864 26-27 25.260683138491697 23.01045193279758 24.100655988483805 27.628208940226923 28-29 25.19639667719994 22.64226583630842 24.238559163025133 27.922778323466506 30-31 25.355551066986425 22.761522663913375 24.195620500821786 27.687305768278414 32-33 25.35298546117212 22.797733511442537 24.390021410851382 27.45925961653396 34-35 25.46483866103903 22.571400368161115 24.306443687295186 27.657317283504668 36-37 25.375072421947735 22.84227683331793 24.46306311586835 27.31958762886598 38-39 25.36828384614207 22.95564344730871 24.48141183618537 27.19466087036385 40-41 25.461925745683665 22.66972309183618 24.496530611235578 27.37182055124458 42-43 25.503838959371993 22.928879462154356 24.4838993287365 27.083382249737152 44-45 25.59129200084613 22.96716803380115 24.588235131461975 26.85330483389074 46-47 25.54069426070168 22.76338657009196 24.497051695036223 27.19886747417014 48-49 25.52393488878882 23.008260142529185 24.347581003246745 27.120223965435258 50-51 25.650572720626062 23.038296460776085 24.454527607029704 26.856603211568142 52-53 25.73819761319761 22.729466479466478 24.449185386685386 27.083150520650523 54-55 25.763749880850252 22.909120515997838 24.61355765259111 26.713571950560798 56-57 25.894200442040137 23.099952592169267 24.365243782322292 26.640603183468304 58-59 25.914485989062435 22.883306586005894 24.363220014744613 26.83898741018706 60-61 25.91333500438651 23.10100472072524 24.37413836320341 26.61152191168484 62-63 26.127775512151953 22.94567993359306 24.29869239646331 26.627852157791683 64-65 26.204744512605938 22.828655334594842 24.16484263936482 26.801757513434403 66-67 26.237196278565623 22.837322735073613 24.13404389847349 26.79143708788727 68-69 26.347285847736607 22.89784246617253 24.08491439620463 26.66995728988623 70-71 26.58870481228827 22.66323263260476 23.67302427026776 27.075038284839202 72-73 26.506935015840057 22.927348720815697 23.823443281952986 26.742272981391263 74-75 26.473911639804303 22.814587627585865 23.719174510144782 26.992326222465046 76-77 27.082083766110276 22.64215808183507 23.13881533712714 27.13694281492751 78-79 26.92550505050505 22.869318181818183 22.971029741863074 27.234147025813694 80-81 27.216855948684337 22.856743776256835 23.135025947932224 26.791374327126604 82-83 27.741813158536253 22.16067607464991 22.442373845445434 27.6551369213684 84-85 27.959769602510832 22.030422454838885 21.723701339229986 28.286106603420297 86-87 28.748769964423587 21.38369540534403 21.23735271112457 28.630181919107816 88-89 28.55205293658296 20.089597632649706 20.66088529037031 30.697464140397024 90-91 24.143099830118917 21.24512841011292 24.053162786049764 30.5586089737184 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 0.5 8 0.0 9 0.5 10 1.5 11 6.0 12 10.0 13 11.5 14 16.0 15 27.5 16 46.0 17 68.0 18 94.0 19 123.0 20 174.0 21 259.0 22 344.5 23 473.5 24 659.0 25 847.0 26 1050.5 27 1328.5 28 1742.0 29 2245.0 30 2790.0 31 3399.5 32 4184.0 33 5033.5 34 6030.0 35 7247.0 36 8587.5 37 10051.5 38 11717.5 39 13733.5 40 15788.0 41 17791.5 42 19842.0 43 21881.0 44 23976.5 45 26088.0 46 28060.5 47 29733.0 48 31077.5 49 32342.5 50 33566.0 51 34411.0 52 34674.5 53 34530.5 54 34182.0 55 33730.0 56 33172.0 57 32325.0 58 31232.0 59 30042.0 60 28614.0 61 27219.0 62 25989.0 63 24376.5 64 22518.5 65 20776.0 66 18998.0 67 17409.5 68 15634.5 69 13620.0 70 11758.0 71 9899.0 72 8160.0 73 6596.0 74 5218.5 75 4134.5 76 3231.0 77 2405.0 78 1843.5 79 1402.5 80 982.0 81 691.5 82 509.0 83 363.0 84 254.5 85 179.5 86 125.5 87 93.0 88 66.5 89 42.5 90 30.0 91 25.0 92 19.5 93 12.0 94 5.5 95 3.0 96 4.0 97 5.0 98 4.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010253024129466986 2 0.008407479786162929 3 6.151814477680192E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 6977.0 32-33 15590.0 34-35 17025.0 36-37 18421.0 38-39 20055.0 40-41 21008.0 42-43 21535.0 44-45 21787.0 46-47 22124.0 48-49 22137.0 50-51 22021.0 52-53 21877.0 54-55 21034.0 56-57 20234.0 58-59 19615.0 60-61 18747.0 62-63 18059.0 64-65 16961.0 66-67 16034.0 68-69 15117.0 70-71 13985.0 72-73 13578.0 74-75 12410.0 76-77 11606.0 78-79 10649.0 80-81 9823.0 82-83 9120.0 84-85 8331.0 86-87 7845.0 88-89 7209.0 90-91 6747.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80104699573322 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80760341292046 99.60903318744376 2 0.18638755924829256 0.3720334711991771 3 0.005366023079061821 0.016066041644809133 4 3.4200809777662066E-4 0.0013653106495650335 5 3.00996654422304E-4 0.0015019890626779414 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0014354233781253781 0.0 0.0 0.0 0.0 2 0.0014354233781253781 0.0 0.0 0.0 0.0 3 0.0014354233781253781 0.0 0.0 0.0 0.0 4 0.0014354233781253781 0.0 0.0 0.0 0.0 5 0.0014354233781253781 0.0 0.0 0.0 0.0 6 0.0014354233781253781 0.0 0.0 0.0 0.0 7 0.0018455443433040575 0.0 0.0 0.0 0.0 8 0.009022661233930949 0.0 0.0 0.0 0.0 9 0.009227721716520287 0.0 0.0 0.0 0.0 10-11 0.009227721716520287 0.0 0.0 0.0 0.0 12-13 0.009227721716520287 0.0 1.0253024129466987E-4 0.0 0.0 14-15 0.009227721716520287 0.0 2.0506048258933974E-4 0.0 0.0 16-17 0.009432782199109627 1.0253024129466987E-4 2.0506048258933974E-4 0.0 0.0 18-19 0.009842903164288307 2.0506048258933974E-4 2.0506048258933974E-4 0.0 0.0 20-21 0.009842903164288307 2.0506048258933974E-4 2.0506048258933974E-4 0.0 0.0 22-23 0.009842903164288307 2.0506048258933974E-4 2.0506048258933974E-4 0.0 0.0 24-25 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2.0506048258933974E-4 2.0506048258933974E-4 0.0 2.0506048258933974E-4 48-49 0.013021340644423073 2.0506048258933974E-4 2.0506048258933974E-4 0.0 4.101209651786795E-4 50-51 0.013226401127012413 2.0506048258933974E-4 2.0506048258933974E-4 0.0 4.101209651786795E-4 52-53 0.013431461609601751 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 54-55 0.013739052333485761 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 56-57 0.013944112816075101 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 58-59 0.013944112816075101 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 60-61 0.013944112816075101 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 62-63 0.013944112816075101 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 64-65 0.013944112816075101 2.0506048258933974E-4 2.0506048258933974E-4 0.0 6.151814477680192E-4 66-67 0.013944112816075101 2.0506048258933974E-4 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