##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-13B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 349365 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.23081018419132 40.0 40.0 40.0 40.0 40.0 2 39.106252772887956 40.0 40.0 40.0 40.0 40.0 3 39.10645313640462 40.0 40.0 40.0 40.0 40.0 4 39.042331086399614 40.0 40.0 40.0 40.0 40.0 5 39.18811558112576 40.0 40.0 40.0 40.0 40.0 6 39.25076639045125 40.0 40.0 40.0 40.0 40.0 7 39.26696721194167 40.0 40.0 40.0 40.0 40.0 8 39.28497702975398 40.0 40.0 40.0 40.0 40.0 9 39.31818012680148 40.0 40.0 40.0 40.0 40.0 10-11 39.316147868275294 40.0 40.0 40.0 40.0 40.0 12-13 39.31571279321054 40.0 40.0 40.0 40.0 40.0 14-15 39.325364590041936 40.0 40.0 40.0 40.0 40.0 16-17 39.470582342249514 40.0 40.0 40.0 40.0 40.0 18-19 39.48540351781088 40.0 40.0 40.0 40.0 40.0 20-21 39.499772444291786 40.0 40.0 40.0 40.0 40.0 22-23 39.49822678287751 40.0 40.0 40.0 40.0 40.0 24-25 39.50588639388605 40.0 40.0 40.0 40.0 40.0 26-27 39.490910652183246 40.0 40.0 40.0 40.0 40.0 28-29 39.4868690338185 40.0 40.0 40.0 40.0 40.0 30-31 39.47807362487834 40.0 40.0 40.0 40.0 40.0 32-33 39.475091379447626 40.0 40.0 40.0 40.0 40.0 34-35 39.47047532828423 40.0 40.0 40.0 40.0 40.0 36-37 39.46410461385321 40.0 40.0 40.0 40.0 40.0 38-39 39.45380807769261 40.0 40.0 40.0 40.0 40.0 40-41 39.43509940617302 40.0 40.0 40.0 40.0 40.0 42-43 39.439910121945395 40.0 40.0 40.0 40.0 40.0 44-45 39.4211781180135 40.0 40.0 40.0 40.0 40.0 46-47 39.41938201479118 40.0 40.0 40.0 40.0 40.0 48-49 39.422751631967174 40.0 40.0 40.0 40.0 40.0 50-51 39.41236215956303 40.0 40.0 40.0 40.0 40.0 52-53 39.40047524075851 40.0 40.0 40.0 40.0 40.0 54-55 39.37401275193885 40.0 40.0 40.0 40.0 40.0 56-57 39.37766963969932 40.0 40.0 40.0 40.0 40.0 58-59 39.36354281052027 40.0 40.0 40.0 40.0 40.0 60-61 39.32709199271684 40.0 40.0 40.0 40.0 40.0 62-63 39.331068738590766 40.0 40.0 40.0 40.0 40.0 64-65 39.329311514088715 40.0 40.0 40.0 40.0 40.0 66-67 39.314258498357205 40.0 40.0 40.0 40.0 40.0 68-69 39.330975622305544 40.0 40.0 40.0 40.0 40.0 70-71 39.321511284322234 40.0 40.0 40.0 40.0 40.0 72-73 39.32237703458997 40.0 40.0 40.0 40.0 40.0 74-75 39.31236500419432 40.0 40.0 40.0 40.0 40.0 76-77 39.30766035010802 40.0 40.0 40.0 40.0 40.0 78-79 39.28821586509069 40.0 40.0 40.0 40.0 40.0 80-81 39.27914020939417 40.0 40.0 40.0 40.0 40.0 82-83 39.289949325104644 40.0 40.0 40.0 40.0 40.0 84-85 39.27253262791669 40.0 40.0 40.0 40.0 40.0 86-87 39.30796496129667 40.0 40.0 40.0 40.0 40.0 88-89 39.32753653584669 40.0 40.0 40.0 40.0 40.0 90-91 39.42477006978787 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3551212693133081 1101 2 0.28519117569425134 1101 3 0.021350828762628282 1101 4 0.2412368468505548 1101 5 0.04513676362059016 1101 6 -0.11840224918267239 1101 7 0.08346855273261156 1101 8 0.07684575580012165 1101 9 -0.11308594329190669 1101 10-11 -0.0754018291670846 1101 12-13 -0.10322009285438583 1101 14-15 9.015307141382323E-4 1101 16-17 0.04560288788097466 1101 18-19 -0.0751239361378353 1101 20-21 -0.0706470499278069 1101 22-23 -0.032660679119523195 1101 24-25 0.04699563127719841 1101 26-27 0.04407595551560206 1101 28-29 -0.03744387404229599 1101 30-31 -0.09121784602977101 1101 32-33 0.06908106102249434 1101 34-35 0.15439990792434344 1101 36-37 0.06154148202100629 1101 38-39 0.07334856371236498 1101 40-41 0.12621920415999455 1101 42-43 0.001673692907701252 1101 44-45 0.09632747318227075 1101 46-47 0.13220519516892182 1101 48-49 -0.14397637945479147 1101 50-51 0.19233296151384138 1101 52-53 0.008010008620843223 1101 54-55 -0.10935070761225063 1101 56-57 0.03301819844568854 1101 58-59 0.05118438116241464 1101 60-61 -0.20400336980319622 1101 62-63 -0.36501798095051896 1101 64-65 0.07991694487073886 1101 66-67 -0.17793252725971342 1101 68-69 -0.06314726916104263 1101 70-71 -0.24681872629729895 1101 72-73 0.012188822923626219 1101 74-75 -0.292710523920384 1101 76-77 -0.05878169747747819 1101 78-79 0.06373427375226015 1101 80-81 -0.41797946798994445 1101 82-83 -0.7447989460366813 1101 84-85 -0.1777867747961679 1101 86-87 -0.27548874086984654 1101 88-89 0.20693295213087026 1101 90-91 0.4045722194765915 1102 1 0.5038649879062689 1102 2 0.3048922451440603 1102 3 0.22459783301465563 1102 4 0.039690654349591625 1102 5 0.23093756248671582 1102 6 0.18975391625992444 1102 7 0.23203188220046655 1102 8 0.10009018306464412 1102 9 0.07928617370332347 1102 10-11 0.1682909939227244 1102 12-13 -0.0042766577300454856 1102 14-15 -2.581136615802393E-4 1102 16-17 0.1332333478732437 1102 18-19 0.13229778786680413 1102 20-21 0.11462546650048466 1102 22-23 0.05625827462355204 1102 24-25 0.01837818507181055 1102 26-27 0.08842591041831582 1102 28-29 -0.14958148517489178 1102 30-31 -0.05854164450934718 1102 32-33 0.09575935972390681 1102 34-35 0.18098975712202048 1102 36-37 0.03546081835300896 1102 38-39 0.02751360284251092 1102 40-41 0.041803182019179985 1102 42-43 0.08907993190972974 1102 44-45 0.06854657860426983 1102 46-47 -0.03317806826711234 1102 48-49 0.0022409322828664813 1102 50-51 0.05404889875137542 1102 52-53 0.11447012706860704 1102 54-55 0.026898481975564437 1102 56-57 0.06260263909164365 1102 58-59 0.14264229420641783 1102 60-61 0.20603706664368815 1102 62-63 -0.03388839616620487 1102 64-65 -0.02475760901391766 1102 66-67 0.09555481812786581 1102 68-69 0.05135198640427063 1102 70-71 0.11141512020336108 1102 72-73 0.0020341796747302965 1102 74-75 -0.02014549201225435 1102 76-77 -0.1621411368139789 1102 78-79 -0.07374569138004006 1102 80-81 0.25100156675161145 1102 82-83 0.11465025648652727 1102 84-85 -0.07436346999036658 1102 86-87 -0.4941648638012879 1102 88-89 -0.825726980529069 1102 90-91 -0.39542778052340566 1103 1 0.334868104756346 1103 2 0.27006621626549077 1103 3 0.13858549283273192 1103 4 0.08836440283887015 1103 5 -0.016336783928906584 1103 6 -0.026873524742555333 1103 7 0.18778533299875733 1103 8 0.11047120208379368 1103 9 0.0745154848158478 1103 10-11 0.10622751195592173 1103 12-13 -0.029834828329882157 1103 14-15 0.004601628085126208 1103 16-17 0.034015740850783516 1103 18-19 0.07385366853597475 1103 20-21 -0.08087100318973484 1103 22-23 -0.053562983566131095 1103 24-25 0.0020751509136829327 1103 26-27 -0.0767289779475604 1103 28-29 0.01503908603559978 1103 30-31 0.005309255560618453 1103 32-33 -0.0728708592138787 1103 34-35 0.020184401004776475 1103 36-37 0.044357400575925965 1103 38-39 -0.17829312362952265 1103 40-41 -0.03410408634675832 1103 42-43 -0.06391415920219146 1103 44-45 -0.11437445752855524 1103 46-47 -0.1065276755582687 1103 48-49 -0.011648410865483072 1103 50-51 -0.10930739837529302 1103 52-53 -0.010549042695863875 1103 54-55 0.09510634962948927 1103 56-57 -0.07867633158172538 1103 58-59 0.06988937110671856 1103 60-61 -0.39462492910041647 1103 62-63 -0.4022686404415907 1103 64-65 -0.18831976826801622 1103 66-67 -0.2925872964357552 1103 68-69 -0.29028966924162347 1103 70-71 -0.3081710432357099 1103 72-73 -0.10080259139733272 1103 74-75 -0.34771317290960013 1103 76-77 -0.4679973518721354 1103 78-79 -0.7614067307709647 1103 80-81 -0.5182817217516913 1103 82-83 -0.7286573278224182 1103 84-85 -0.04563505554767033 1103 86-87 -0.0741546934478734 1103 88-89 -0.3621072031161816 1103 90-91 0.4045722194765915 1104 1 0.4390718865972545 1104 2 0.14710950427924274 1104 3 0.17177817918997818 1104 4 0.011227350174394246 1104 5 -0.08040929525880358 1104 6 -0.10738610483319633 1104 7 0.05782752709158956 1104 8 0.02708317270420224 1104 9 0.14940218586288267 1104 10-11 -0.1242147446466646 1104 12-13 -0.20948789912219468 1104 14-15 -0.07222287574359854 1104 16-17 -0.05496691268882614 1104 18-19 -0.03922650024039598 1104 20-21 -0.11784553045961843 1104 22-23 -0.10122668101885068 1104 24-25 -0.12147540386050082 1104 26-27 0.004189915629559948 1104 28-29 -0.03943817603660449 1104 30-31 0.013435525290262262 1104 32-33 -0.10623732155266907 1104 34-35 -0.030769155386984437 1104 36-37 -0.06973988571546386 1104 38-39 0.02699749885029945 1104 40-41 -0.03910662853348157 1104 42-43 -0.023706260420034653 1104 44-45 -0.08228911295704222 1104 46-47 -0.034779281218838776 1104 48-49 0.06695501442656848 1104 50-51 0.004254386953498113 1104 52-53 -0.12314900550206431 1104 54-55 0.21708025234797645 1104 56-57 -0.0518661280029562 1104 58-59 0.009015676899693403 1104 60-61 0.07033775282609156 1104 62-63 0.31465144679253854 1104 64-65 0.06549872557958736 1104 66-67 0.09666299068040018 1104 68-69 0.09616927976710343 1104 70-71 -0.07461314239855454 1104 72-73 0.04305741104209915 1104 74-75 0.3062285446106685 1104 76-77 -0.25329908625847963 1104 78-79 -0.30308739584829425 1104 80-81 -0.08318137451878727 1104 82-83 -0.11432221628517425 1104 84-85 -0.11428058626216142 1104 86-87 -0.09474965912292532 1104 88-89 -0.8243886674260494 1104 90-91 -1.3954277805234057 1201 1 0.28652223549688216 1201 2 0.44587493637801145 1201 3 0.2741936381271728 1201 4 -0.04051714794691463 1201 5 0.12689075841806385 1201 6 0.0698549042918728 1201 7 0.23657148844424114 1201 8 0.3322898286354956 1201 9 0.021436539056658432 1201 10-11 -0.04407518479696648 1201 12-13 0.013056384291651568 1201 14-15 0.12799183171748751 1201 16-17 0.008479142061574407 1201 18-19 0.08106335483206095 1201 20-21 0.026491560254278568 1201 22-23 -0.03708282701992971 1201 24-25 -0.03312328362432737 1201 26-27 0.027056260235035268 1201 28-29 0.16329934409222346 1201 30-31 0.07307052260642166 1201 32-33 0.1273765614711735 1201 34-35 -0.09631659641625845 1201 36-37 0.08529854109640667 1201 38-39 0.06618318085860864 1201 40-41 -0.11009518000338403 1201 42-43 0.06187100132471102 1201 44-45 -0.013263271749337946 1201 46-47 0.10072190287586125 1201 48-49 -0.12149719383666735 1201 50-51 0.16581397987341973 1201 52-53 0.1593784144305701 1201 54-55 0.11387141026204972 1201 56-57 -0.07261213688571644 1201 58-59 -0.015564211759389934 1201 60-61 -0.018173121878106713 1201 62-63 -0.1650529369831304 1201 64-65 0.056524497121401396 1201 66-67 0.11227848274052121 1201 68-69 -0.13001237757205075 1201 70-71 0.10460766690574985 1201 72-73 -0.5170819590448872 1201 74-75 0.08922548795913343 1201 76-77 -0.5105441667593738 1201 78-79 -0.5389089918987793 1201 80-81 0.24373611677489748 1201 82-83 -0.5801522868256157 1201 84-85 -0.3132081604089123 1201 86-87 -0.6537608233972492 1201 88-89 0.57056931576723 1201 90-91 -1.5954277805234085 1202 1 0.05286741403453732 1202 2 0.13112602376433102 1202 3 -0.07084736450318019 1202 4 0.1576708280532202 1202 5 0.034278108633820636 1202 6 0.07654254505037272 1202 7 0.11239454963013884 1202 8 0.012022323005155044 1202 9 0.03838773131343487 1202 10-11 -0.026565256487103284 1202 12-13 0.15566301691840323 1202 14-15 0.01468016015112994 1202 16-17 0.048604986612602374 1202 18-19 0.0765504867109641 1202 20-21 0.1159666922019511 1202 22-23 0.09850092321645576 1202 24-25 -0.03078518814063358 1202 26-27 0.06632251085823526 1202 28-29 0.0365866031910258 1202 30-31 0.05755485220912249 1202 32-33 0.02261064943886737 1202 34-35 -0.05183307572525564 1202 36-37 -0.05812120514044494 1202 38-39 -0.06780411831861244 1202 40-41 0.053702896713360815 1202 42-43 -0.07331239791501787 1202 44-45 -0.1377767664564118 1202 46-47 -0.048293358503613604 1202 48-49 0.024901538299886283 1202 50-51 0.08870040935203605 1202 52-53 -0.09069058215877845 1202 54-55 0.02860757863999197 1202 56-57 0.013292481243318832 1202 58-59 0.15729613966038158 1202 60-61 0.08650332234513769 1202 62-63 -0.21031363223411859 1202 64-65 0.1245146643129118 1202 66-67 -0.021458327452982928 1202 68-69 -0.1814975791922322 1202 70-71 0.12294739520775266 1202 72-73 -0.16051153310746002 1202 74-75 -0.17541206264332487 1202 76-77 0.06190338167233023 1202 78-79 0.5583948421267948 1202 80-81 0.0663249419020957 1202 82-83 0.19415498115253627 1202 84-85 0.307955513129329 1202 86-87 0.15781571658047966 1202 88-89 -0.5881608429629281 1202 90-91 0.4045722194765915 1203 1 0.17287274860324686 1203 2 0.05481642224257399 1203 3 -0.08752491088234393 1203 4 0.07910666933576493 1203 5 -0.08701313776008135 1203 6 0.09698236620194223 1203 7 -0.01396734470328198 1203 8 0.103670213393805 1203 9 -0.031034661240624928 1203 10-11 0.017694817775719685 1203 12-13 -0.16968952086227773 1203 14-15 -0.18489728585391418 1203 16-17 -0.09700066241488514 1203 18-19 -0.09287541542777689 1203 20-21 -0.20496657655502304 1203 22-23 -0.2224042688631087 1203 24-25 -0.13604184406796804 1203 26-27 -0.03205816677237294 1203 28-29 -0.016733814870704578 1203 30-31 -0.10936380263726164 1203 32-33 -0.03132361920655313 1203 34-35 -0.15083784491783092 1203 36-37 -0.1407445183706173 1203 38-39 -0.154203757617573 1203 40-41 -0.14201048942898353 1203 42-43 -0.19032853990233178 1203 44-45 -0.0785939030066487 1203 46-47 0.016034246743998892 1203 48-49 0.052048414523781616 1203 50-51 -0.1232372343537449 1203 52-53 -0.23978485891327495 1203 54-55 -0.17427823911302198 1203 56-57 -0.377066468512659 1203 58-59 -0.18979534814084076 1203 60-61 -0.17496329182433357 1203 62-63 -0.39063561542234737 1203 64-65 -0.40136358660486593 1203 66-67 -0.4237393414838664 1203 68-69 -0.7140250916216999 1203 70-71 -0.5113117768727236 1203 72-73 0.006868903909115431 1203 74-75 -0.48409745619714073 1203 76-77 -0.6500010624472736 1203 78-79 -0.023791230191378077 1203 80-81 -0.19350803299219876 1203 82-83 0.16374306234493474 1203 84-85 0.11727870412065755 1203 86-87 0.3391684695320478 1203 88-89 -0.270456325258408 1203 90-91 0.4045722194765915 1204 1 0.1177078743941351 1204 2 -0.12122796328839769 1204 3 -0.04551717621161799 1204 4 0.05438305399214727 1204 5 -0.1119663334363139 1204 6 -0.08209029324023476 1204 7 -0.3748129655950976 1204 8 -0.185912579013646 1204 9 -0.2394553398691599 1204 10-11 -0.024143464744753373 1204 12-13 -0.10947934553396266 1204 14-15 -0.1866983982252819 1204 16-17 0.07063397688434492 1204 18-19 -0.002428963673807516 1204 20-21 -0.0395402814076391 1204 22-23 -0.259924254650997 1204 24-25 -0.051375897117424074 1204 26-27 -0.012215866596086755 1204 28-29 -0.10560873458591402 1204 30-31 -0.026777744176278873 1204 32-33 -0.08839649604609434 1204 34-35 -0.04459245354327379 1204 36-37 -0.03630987824080023 1204 38-39 -0.08065035552676392 1204 40-41 -0.03190509946252007 1204 42-43 -0.03150755682477069 1204 44-45 -0.22715711419424167 1204 46-47 -0.10282768008472942 1204 48-49 -0.027161555563736783 1204 50-51 -0.13414958918548336 1204 52-53 -0.18188993594105085 1204 54-55 -0.3348207030550867 1204 56-57 -0.15499393050030363 1204 58-59 -0.2170849453452135 1204 60-61 -0.039597279657670015 1204 62-63 -0.2516015871867694 1204 64-65 -0.21343496173815169 1204 66-67 -0.07710095525769844 1204 68-69 -0.0535455547956758 1204 70-71 0.041559914439467605 1204 72-73 -0.2781961269412321 1204 74-75 0.021387727633111808 1204 76-77 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0.22159544888707217 2401 60-61 0.13821422617812118 2401 62-63 0.1822000335048699 2401 64-65 0.10221716992465701 2401 66-67 0.09666463484475685 2401 68-69 0.25260620173522597 2401 70-71 0.38034282834973965 2401 72-73 0.054327727737792486 2401 74-75 0.23903949721088935 2401 76-77 -0.0251757898724847 2401 78-79 -0.017872024439924417 2401 80-81 0.2929314869648536 2401 82-83 0.1750600151549193 2401 84-85 -0.03909499366780267 2401 86-87 0.4524653729928829 2401 88-89 0.027822487306110588 2401 90-91 0.4045722194765915 2402 1 0.181405458772943 2402 2 0.40999916734680397 2402 3 0.2904943603477861 2402 4 0.1771223089534928 2402 5 0.132288882126943 2402 6 0.13598272541577217 2402 7 0.14277304632663146 2402 8 0.2796202550389282 2402 9 0.17373775113685497 2402 10-11 0.05823940173898734 2402 12-13 0.2325747105187972 2402 14-15 0.13817253189441914 2402 16-17 0.059237618727173924 2402 18-19 -0.003558276980449193 2402 20-21 0.06001954831717171 2402 22-23 0.06775840368782582 2402 24-25 0.07933171858326205 2402 26-27 0.01349488437414692 2402 28-29 0.003039525146903088 2402 30-31 0.04743185205954603 2402 32-33 0.13552325445673574 2402 34-35 0.12991876486032083 2402 36-37 0.07579481534371979 2402 38-39 0.14409027061928015 2402 40-41 0.01168759100563932 2402 42-43 0.004423612035829194 2402 44-45 0.08841048881217262 2402 46-47 0.059129480258334866 2402 48-49 0.011391809328280544 2402 50-51 0.10642234150923713 2402 52-53 0.06686031853787 2402 54-55 0.18377050660497218 2402 56-57 0.12689047503346984 2402 58-59 0.14414090124411416 2402 60-61 0.21723064882058907 2402 62-63 0.04057016659892554 2402 64-65 0.052816628384270814 2402 66-67 0.10892981244081312 2402 68-69 0.2961279566569601 2402 70-71 -0.06887668255000534 2402 72-73 0.27381749758431084 2402 74-75 0.2892782352755674 2402 76-77 0.29089822409661537 2402 78-79 0.28311797516549575 2402 80-81 0.21072834572103716 2402 82-83 0.48838297538053155 2402 84-85 0.34543931350835066 2402 86-87 -0.4877321152632774 2402 88-89 -0.2627640175661057 2402 90-91 -0.48431666941229423 2403 1 -0.22547381477465933 2403 2 -0.041116582955034175 2403 3 -0.03819738498998504 2403 4 0.08979889282527864 2403 5 0.2117536740926127 2403 6 0.04742519633428799 2403 7 0.20444244731582017 2403 8 -0.03776598002765752 2403 9 0.19772441926489392 2403 10-11 0.18301857750687844 2403 12-13 0.16769575283506555 2403 14-15 0.13693496687694307 2403 16-17 0.02698411984435012 2403 18-19 0.043912783933812705 2403 20-21 0.04171238882231876 2403 22-23 0.15771315575625522 2403 24-25 0.08430614310258022 2403 26-27 0.06710975655716567 2403 28-29 0.07403985568204519 2403 30-31 0.03765733226325807 2403 32-33 -0.030624569601165774 2403 34-35 0.06340361961939323 2403 36-37 0.08239622290793847 2403 38-39 0.028883087099359273 2403 40-41 -0.018212354104228723 2403 42-43 0.19724406260575478 2403 44-45 0.1355532275664686 2403 46-47 0.06363792351451991 2403 48-49 0.034225697228670526 2403 50-51 -0.039793797208119486 2403 52-53 -0.04286170085355678 2403 54-55 0.06952804076771457 2403 56-57 0.036066793755864524 2403 58-59 -0.011597924666290282 2403 60-61 0.16083700137319568 2403 62-63 0.44900740832545694 2403 64-65 0.15537323592462826 2403 66-67 0.12988334235933507 2403 68-69 0.30625920404180107 2403 70-71 0.2975717293223852 2403 72-73 0.34296136394556953 2403 74-75 0.2001197155200245 2403 76-77 0.35561358045023184 2403 78-79 0.3786974629499156 2403 80-81 0.3391935043610914 2403 82-83 0.5307093796264439 2403 84-85 0.25476824982290935 2403 86-87 0.5934582023027914 2403 88-89 0.4302184385742436 2403 90-91 -0.2620944471900799 2404 1 -0.3779150133092273 2404 2 -0.30637062554291816 2404 3 -0.2756084818185798 2404 4 -0.2934838112310203 2404 5 -0.07441798674256006 2404 6 -0.041293107528659334 2404 7 -0.06735812960265974 2404 8 0.15273405167568654 2404 9 0.13454336353535723 2404 10-11 0.036778882122476375 2404 12-13 0.1964436849125093 2404 14-15 0.1228718582859969 2404 16-17 0.02226155702850008 2404 18-19 0.015430830546783625 2404 20-21 0.10154606567714808 2404 22-23 0.13158877819167714 2404 24-25 0.05652083978394273 2404 26-27 0.10201390129568466 2404 28-29 0.031307044040254084 2404 30-31 -0.03464111064972997 2404 32-33 0.06714586696548253 2404 34-35 0.018190381971649572 2404 36-37 0.011532144602945493 2404 38-39 0.10617462636634656 2404 40-41 0.19957779542089327 2404 42-43 0.14827614933587796 2404 44-45 0.05669953354428969 2404 46-47 0.09348196595040292 2404 48-49 0.09528926071872235 2404 50-51 0.04293700081304763 2404 52-53 0.20387551819004557 2404 54-55 0.062156645647007736 2404 56-57 0.10179587655799338 2404 58-59 0.20460882983788764 2404 60-61 0.1002036550762142 2404 62-63 0.3615765230059367 2404 64-65 0.18723563699654733 2404 66-67 0.1521860659055534 2404 68-69 0.21862302124866773 2404 70-71 0.3720763017853628 2404 72-73 0.23460075559219007 2404 74-75 0.5212947660843881 2404 76-77 0.17063404090914247 2404 78-79 0.3406347699735335 2404 80-81 0.448584538682951 2404 82-83 0.356550677243888 2404 84-85 -0.21041789175907866 2404 86-87 0.22157768892756735 2404 88-89 0.57056931576723 2404 90-91 0.4045722194765915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 10.0 31 213.0 32 1472.0 33 2627.0 34 3535.0 35 4399.0 36 6067.0 37 9473.0 38 24538.0 39 149725.0 40 147306.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.944335515412426 13.806349306304941 28.779400104762697 30.469915073519942 2 25.59634734567106 18.167661413829133 29.23583379850287 27.000157441996937 3 26.734600412182274 19.974238607739867 22.133615754522555 31.1575452255553 4 26.676971076095203 21.317819472471484 20.93798749159189 31.06722195984143 5 27.050219684284343 21.166115666995836 20.76939590399725 31.014268744722568 6 27.43720750504487 23.1840625134172 23.53040516365406 25.848324817883878 7 26.059565211168835 23.304566857012006 22.644225952800078 27.99164197901908 8 25.10068266712464 24.467247720865 23.126815794369783 27.30525381764058 9 24.623245030269203 24.65473072574528 23.71416713179626 27.00785711218926 10-11 24.501595752293447 23.478883116511383 23.619566928570407 28.399954202624762 12-13 24.860532680720738 23.196227441214777 23.520959455011234 28.42228042305325 14-15 25.35571680048087 22.95736550598944 23.12853319594121 28.55838449758848 16-17 25.388776780730755 22.69217008000229 22.913714882715787 29.005338256551173 18-19 25.562377456242043 23.009030669929732 22.697465401514176 28.731126472314056 20-21 25.57425615044438 23.162738110572036 22.641936084038182 28.6210696549454 22-23 25.358436019635626 22.900691254132497 22.74884433185923 28.99202839437265 24-25 25.477509195254243 23.1431311092983 22.829848439311323 28.549511256136135 26-27 25.693329326063 23.140698123738783 22.820402730668498 28.34556981952972 28-29 25.64967870278935 22.786340932835287 22.975398222489375 28.588582141885993 30-31 25.72449611937137 23.04505193015298 23.03932723366923 28.19112471680642 32-33 25.87468358313164 23.14250700024393 22.908946435900845 28.073862980723586 34-35 25.686855213679078 22.99097958537743 23.183445240732787 28.1387199602107 36-37 25.717620551346332 23.24932773122374 23.37589082448824 27.657160892941686 38-39 25.81438389804873 23.298454012314558 23.269668001340094 27.617494088296617 40-41 25.79975779347131 22.8888571389694 23.39929379107646 27.912091276482837 42-43 25.905599814025017 23.180708671977683 23.50455549296782 27.40913602102948 44-45 26.003930207666738 23.335088901599455 23.400781133493034 27.260199757240777 46-47 25.92821633564687 23.153373840229914 23.414547259380228 27.503862564742988 48-49 25.97828148002957 23.389699788439373 23.36051166566177 27.271507065869283 50-51 26.102603700438927 23.326932844982906 23.43717613187284 27.133287322705335 52-53 26.00166271364648 23.12272293811282 23.43423723011468 27.441377118126027 54-55 26.143851116912952 23.45872862400645 23.393918956300897 27.003501302779704 56-57 26.332053589737747 23.432978650686962 23.31962450727973 26.915343252295564 58-59 26.295597873129715 23.22214665512551 23.41041177197972 27.071843699765054 60-61 26.347139959705405 23.48036143847662 23.352335132439844 26.820163469378127 62-63 26.439899756033647 23.45361871122282 23.19488888642831 26.911592646315224 64-65 26.522034560346334 23.406151204566818 23.11441858951146 26.95739564557539 66-67 26.496652047575214 23.745645857021678 23.128846535636292 26.62885555976682 68-69 26.74642664882297 23.714675266523447 22.71952023263887 26.819377852014707 70-71 26.797448454482737 23.288507866134342 23.014804845222073 26.899238834160847 72-73 26.577428024019856 23.628309480641388 23.11975208630688 26.674510409031875 74-75 26.704205369216787 23.662683721651998 22.670272746754158 26.962838162377057 76-77 26.97843890450042 23.57283422276075 22.6593450033085 26.789381869430333 78-79 27.00180920049601 23.63141097310593 22.62923586689163 26.737543959506432 80-81 27.12299176454705 23.750260809799084 22.234495624532077 26.89225180112179 82-83 27.64406883458877 23.364872313062648 21.945618225243432 27.04544062710515 84-85 28.37537759817553 23.110008602236583 21.289535279172586 27.22507852041531 86-87 29.235964542000843 22.07963979175461 20.697903475446743 27.986492190797808 88-89 27.786577363442998 21.85817079241526 20.762999502194866 29.592252341946875 90-91 25.717987475707186 24.28201252429281 22.263010148995896 27.7369898510041 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 2.0 11 7.0 12 11.5 13 10.0 14 10.0 15 17.5 16 29.0 17 39.5 18 51.5 19 70.5 20 91.5 21 118.0 22 150.5 23 208.0 24 295.5 25 386.5 26 504.0 27 671.5 28 895.5 29 1127.0 30 1384.5 31 1711.5 32 2162.0 33 2670.5 34 3246.0 35 3925.0 36 4731.0 37 5666.0 38 6715.5 39 7903.0 40 9195.0 41 10642.0 42 12138.0 43 13634.0 44 15058.0 45 16574.5 46 18325.5 47 19974.5 48 21240.0 49 22451.5 50 23498.0 51 24169.0 52 24734.0 53 25198.5 54 25493.5 55 25259.0 56 24619.5 57 24058.5 58 23389.5 59 22630.0 60 22081.0 61 21185.5 62 20073.5 63 18966.5 64 17592.0 65 16261.0 66 14657.5 67 13169.5 68 11845.0 69 10290.5 70 8886.5 71 7569.5 72 6285.5 73 5052.0 74 3926.0 75 3062.5 76 2399.0 77 1790.0 78 1330.5 79 981.5 80 667.5 81 439.0 82 290.5 83 191.0 84 130.0 85 89.0 86 58.5 87 41.0 88 30.0 89 19.0 90 11.0 91 6.5 92 3.5 93 1.5 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001144934380948292 2 0.00858700785711219 3 0.0014311679761853649 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 4571.0 32-33 10279.0 34-35 11408.0 36-37 12411.0 38-39 13287.0 40-41 14114.0 42-43 14670.0 44-45 15054.0 46-47 15119.0 48-49 15157.0 50-51 15094.0 52-53 14862.0 54-55 14240.0 56-57 13990.0 58-59 13431.0 60-61 13033.0 62-63 12648.0 64-65 12196.0 66-67 11609.0 68-69 11106.0 70-71 10703.0 72-73 10312.0 74-75 9604.0 76-77 9038.0 78-79 8561.0 80-81 8298.0 82-83 7716.0 84-85 7323.0 86-87 6828.0 88-89 6521.0 90-91 6182.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.64504576161869 #Duplication Level Percentage of deduplicated Percentage of total 1 99.66176886532125 99.308015192688 2 0.32179452963609984 0.6413046126285545 3 0.01488569318099028 0.044498567346395755 4 0.0015509118616470652 0.006181627337042362 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.724671904741459E-4 0.0 0.0 0.0 2.8623359523707295E-4 2 5.724671904741459E-4 0.0 0.0 0.0 2.8623359523707295E-4 3 5.724671904741459E-4 0.0 0.0 0.0 2.8623359523707295E-4 4 5.724671904741459E-4 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 5 5.724671904741459E-4 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 6 5.724671904741459E-4 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 7 0.0011449343809482918 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 8 0.006583372690452678 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 9 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 10-11 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 12-13 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 14-15 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 16-17 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 18-19 0.006869606285689752 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 20-21 0.007442073476163897 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 22-23 0.0077283070714009704 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 24-25 0.008014540666638044 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 26-27 0.008014540666638044 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 28-29 0.008443891059493652 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 30-31 0.008730124654730727 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 32-33 0.009016358249967799 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 34-35 0.009731942238060481 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 36-37 0.009731942238060481 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 38-39 0.009731942238060481 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 40-41 0.009875059035679017 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 42-43 0.010447526226153164 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 44-45 0.0105906430237717 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 46-47 0.010733759821390236 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 48-49 0.010876876619008774 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 50-51 0.010876876619008774 0.0 0.0 2.8623359523707295E-4 2.8623359523707295E-4 52-53 0.010876876619008774 0.0 0.0 2.8623359523707295E-4 5.724671904741459E-4 54-55 0.01101999341662731 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 56-57 0.011163110214245845 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 58-59 0.011163110214245845 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 60-61 0.011163110214245845 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 62-63 0.011735577404719992 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 64-65 0.011735577404719992 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 66-67 0.011735577404719992 0.0 0.0 2.8623359523707295E-4 8.58700785711219E-4 68-69 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