##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-13A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 475795 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.254922813396526 40.0 40.0 40.0 40.0 40.0 2 39.129318298847195 40.0 40.0 40.0 40.0 40.0 3 39.11657751762839 40.0 40.0 40.0 40.0 40.0 4 39.070162570014396 40.0 40.0 40.0 40.0 40.0 5 39.200151325675975 40.0 40.0 40.0 40.0 40.0 6 39.23657877867569 40.0 40.0 40.0 40.0 40.0 7 39.26966865982198 40.0 40.0 40.0 40.0 40.0 8 39.312846919366535 40.0 40.0 40.0 40.0 40.0 9 39.33324225769501 40.0 40.0 40.0 40.0 40.0 10-11 39.31336815225045 40.0 40.0 40.0 40.0 40.0 12-13 39.32632331151021 40.0 40.0 40.0 40.0 40.0 14-15 39.33089250622642 40.0 40.0 40.0 40.0 40.0 16-17 39.467009951764936 40.0 40.0 40.0 40.0 40.0 18-19 39.47838880189998 40.0 40.0 40.0 40.0 40.0 20-21 39.493155665780435 40.0 40.0 40.0 40.0 40.0 22-23 39.486997551466494 40.0 40.0 40.0 40.0 40.0 24-25 39.47917905820784 40.0 40.0 40.0 40.0 40.0 26-27 39.472730903014956 40.0 40.0 40.0 40.0 40.0 28-29 39.47371451990878 40.0 40.0 40.0 40.0 40.0 30-31 39.45969967185635 40.0 40.0 40.0 40.0 40.0 32-33 39.463930549934354 40.0 40.0 40.0 40.0 40.0 34-35 39.458572787053214 40.0 40.0 40.0 40.0 40.0 36-37 39.447783266826015 40.0 40.0 40.0 40.0 40.0 38-39 39.43245967256914 40.0 40.0 40.0 40.0 40.0 40-41 39.420523245493484 40.0 40.0 40.0 40.0 40.0 42-43 39.42880423821419 40.0 40.0 40.0 40.0 40.0 44-45 39.40781743205738 40.0 40.0 40.0 40.0 40.0 46-47 39.396279726915 40.0 40.0 40.0 40.0 40.0 48-49 39.400146675596716 40.0 40.0 40.0 40.0 40.0 50-51 39.392835455913 40.0 40.0 40.0 40.0 40.0 52-53 39.38274608610443 40.0 40.0 40.0 40.0 40.0 54-55 39.36819618140951 40.0 40.0 40.0 40.0 40.0 56-57 39.359498801305236 40.0 40.0 40.0 40.0 40.0 58-59 39.340974012730115 40.0 40.0 40.0 40.0 40.0 60-61 39.3103058575478 40.0 40.0 40.0 40.0 40.0 62-63 39.347412429143425 40.0 40.0 40.0 40.0 40.0 64-65 39.35395752735184 40.0 40.0 40.0 40.0 40.0 66-67 39.35454005698632 40.0 40.0 40.0 40.0 40.0 68-69 39.35140953500493 40.0 40.0 40.0 40.0 40.0 70-71 39.33688258214573 40.0 40.0 40.0 40.0 40.0 72-73 39.33888644120317 40.0 40.0 40.0 40.0 40.0 74-75 39.32032516792158 40.0 40.0 40.0 40.0 40.0 76-77 39.319542815776586 40.0 40.0 40.0 40.0 40.0 78-79 39.321502176190734 40.0 40.0 40.0 40.0 40.0 80-81 39.3219313003461 40.0 40.0 40.0 40.0 40.0 82-83 39.305351970379085 40.0 40.0 40.0 40.0 40.0 84-85 39.30089198548961 40.0 40.0 40.0 40.0 40.0 86-87 39.30648967521693 40.0 40.0 40.0 40.0 40.0 88-89 39.346230779087314 40.0 40.0 40.0 40.0 40.0 90-91 39.42393643860008 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33713730706985956 1101 2 0.17965662052903753 1101 3 0.1196892095525044 1101 4 0.22714811316161132 1101 5 0.13897057076830777 1101 6 -0.1041384977258204 1101 7 0.14487359815324652 1101 8 0.047103101310590034 1101 9 0.05218427471708509 1101 10-11 -0.05549773204896269 1101 12-13 -0.029318056757759337 1101 14-15 -0.07368867556046155 1101 16-17 -0.0694267051527575 1101 18-19 0.005158985169337882 1101 20-21 -0.19188088422593097 1101 22-23 0.009038780325113294 1101 24-25 -0.0865692971501204 1101 26-27 -0.07751950186298728 1101 28-29 -0.0647110558004016 1101 30-31 0.07155212892430285 1101 32-33 -0.06223099384813224 1101 34-35 -0.052592727478312895 1101 36-37 0.029106063730736764 1101 38-39 -0.07795264183194206 1101 40-41 -0.03418911637776034 1101 42-43 -0.16083217016962692 1101 44-45 -0.07369453377488355 1101 46-47 -0.08139408663436143 1101 48-49 -0.15842668828196338 1101 50-51 -0.086432937905073 1101 52-53 0.027652332481345354 1101 54-55 -0.16160757863777775 1101 56-57 -0.19904612071009353 1101 58-59 -0.16017697170253342 1101 60-61 -0.15541327381893666 1101 62-63 -0.004706803535832194 1101 64-65 -0.047286414435760094 1101 66-67 -0.33198022196363297 1101 68-69 -0.03623155403642642 1101 70-71 -0.1138997108637767 1101 72-73 -0.047395391299723144 1101 74-75 -0.26444107513401605 1101 76-77 0.23006734797922235 1101 78-79 0.25783368744721713 1101 80-81 -0.0757172217296187 1101 82-83 -0.10724685138458057 1101 84-85 -0.2530489000532157 1101 86-87 -0.33428392806783336 1101 88-89 -0.5341016357484705 1101 90-91 -0.4687062648000193 1102 1 0.4367394770517734 1102 2 0.06032076650964058 1102 3 0.2250385437625937 1102 4 0.03557816412330794 1102 5 0.17880617819885458 1102 6 0.03439183270324264 1102 7 0.1696113920049669 1102 8 0.05413252758052778 1102 9 0.09174370263094289 1102 10-11 0.06429842116491358 1102 12-13 0.08297850743754509 1102 14-15 -0.09324152777153927 1102 16-17 -0.05350364087526316 1102 18-19 0.020608598846798998 1102 20-21 0.16630750965866525 1102 22-23 0.05524953159915924 1102 24-25 0.0649875004826228 1102 26-27 0.01624015620046748 1102 28-29 0.01804087581229652 1102 30-31 -0.019935623829276494 1102 32-33 0.07031431225175311 1102 34-35 0.030128933204601083 1102 36-37 0.07447184443420696 1102 38-39 0.021820773835990792 1102 40-41 -0.011804678156039472 1102 42-43 0.09598567432143312 1102 44-45 0.03155904960218692 1102 46-47 0.08986554451931994 1102 48-49 0.09910965020695528 1102 50-51 0.0432200473654305 1102 52-53 -0.01590062548540061 1102 54-55 -0.19887853049785775 1102 56-57 0.20995858764671027 1102 58-59 0.04600624549922827 1102 60-61 -0.21765744072261128 1102 62-63 0.27094422432316634 1102 64-65 0.30018718718559256 1102 66-67 0.22178131955509883 1102 68-69 0.33021321249221103 1102 70-71 0.45436528497858575 1102 72-73 0.2190764802913705 1102 74-75 0.12585396379803626 1102 76-77 -0.03229052410210187 1102 78-79 0.235508230204033 1102 80-81 0.37835736329734715 1102 82-83 0.17585661129269425 1102 84-85 0.6685312886260277 1102 86-87 -0.14598605572740553 1102 88-89 0.5262069196512513 1102 90-91 0.4062937351999807 1103 1 0.31168276161531594 1103 2 0.3614748023472245 1103 3 0.14514375500704801 1103 4 -0.016488250474751 1103 5 0.17897057076831402 1103 6 0.044952411365088096 1103 7 0.06487359815324822 1103 8 0.15255764676513905 1103 9 0.1794570019898103 1103 10-11 0.07177499522376252 1103 12-13 0.027045579605882608 1103 14-15 0.03358405171226764 1103 16-17 0.11784602211996997 1103 18-19 0.04515898516933703 1103 20-21 0.08630093395589 1103 22-23 -5.2128765801739974E-5 1103 24-25 0.026157975577149273 1103 26-27 0.004298679955198281 1103 28-29 -0.004711055800399322 1103 30-31 6.430380152053772E-4 1103 32-33 0.05794627851802403 1103 34-35 0.05029865898497121 1103 36-37 0.01574236471317647 1103 38-39 -0.1836763320957573 1103 40-41 0.13747018484391305 1103 42-43 -0.003250267495111814 1103 44-45 0.02885977226512182 1103 46-47 0.018281360088522547 1103 48-49 0.1055258655064506 1103 50-51 0.05536115490404114 1103 52-53 0.00963628935605243 1103 54-55 0.18876005409808982 1103 56-57 0.17442194131660216 1103 58-59 -0.037326876419157884 1103 60-61 0.12378482274596081 1103 62-63 -0.16776144113184444 1103 64-65 -0.2550730729713635 1103 66-67 -0.007951467141062096 1103 68-69 -0.36362380184503706 1103 70-71 0.011027570824410304 1103 72-73 0.025115298218260307 1103 74-75 0.03343109817328127 1103 76-77 -0.42199474823255656 1103 78-79 0.011842250930008902 1103 80-81 0.11234979992642025 1103 82-83 -0.3040008157630041 1103 84-85 -1.2083684074226042 1103 86-87 -0.34759895895321336 1103 88-89 -0.2281790452610295 1103 90-91 0.4062937351999807 1104 1 0.23840576927663903 1104 2 0.1467492672394073 1104 3 0.3129030157289492 1104 4 0.03518539299732737 1104 5 0.16546452859154925 1104 6 0.09075606036595474 1104 7 -0.045778009144754606 1104 8 -0.001477579520305028 1104 9 0.142452026079539 1104 10-11 0.06283731257626357 1104 12-13 -0.02610346075428538 1104 14-15 -0.0014179228552961831 1104 16-17 0.026744213416080242 1104 18-19 0.021007654310800206 1104 20-21 0.01952579771072749 1104 22-23 -0.060094779425305944 1104 24-25 -0.1015254617500787 1104 26-27 0.08358783563010519 1104 28-29 -0.10645657353201443 1104 30-31 0.0680105445312833 1104 32-33 -0.027668253295793477 1104 34-35 -0.0024472663205017398 1104 36-37 -0.008502380456654635 1104 38-39 0.08666562454617122 1104 40-41 -0.04999750796436331 1104 42-43 -0.03391960039264319 1104 44-45 -0.10001923156318782 1104 46-47 0.028556578542762168 1104 48-49 -0.09428244693690147 1104 50-51 -0.16333135916952557 1104 52-53 0.009679476744381077 1104 54-55 -0.1272566228004024 1104 56-57 0.0903624561625449 1104 58-59 -0.022965089560578633 1104 60-61 0.14702092574688663 1104 62-63 -0.07224287531240492 1104 64-65 0.16864466875720296 1104 66-67 -0.07460154063471691 1104 68-69 -7.343287505108265E-4 1104 70-71 -0.112143546593785 1104 72-73 -0.09454010252618872 1104 74-75 -0.2041878837645612 1104 76-77 -0.0013854138866520316 1104 78-79 -0.20655763317724052 1104 80-81 0.23330411407742702 1104 82-83 0.09488408312189023 1104 84-85 0.09657443192340764 1104 86-87 0.18342570897847565 1104 88-89 -0.7527899455525144 1104 90-91 0.4062937351999807 1201 1 0.4143786863802035 1201 2 0.1763337365165043 1201 3 0.16896820641770915 1201 4 0.2868346335377865 1201 5 0.09464455195953292 1201 6 0.12357310102026275 1201 7 0.04280463263600609 1201 8 0.13136642419460287 1201 9 0.12936295810266785 1201 10-11 0.019455246007467508 1201 12-13 -0.010352539516375714 1201 14-15 -0.05321845612472487 1201 16-17 0.02549492494128458 1201 18-19 0.021428577645828284 1201 20-21 -8.777494296907662E-4 1201 22-23 0.013427495058650152 1201 24-25 -0.010519140410309546 1201 26-27 -0.1628800034303879 1201 28-29 0.00532029216198282 1201 30-31 0.02684993456693263 1201 32-33 -0.05361038590925915 1201 34-35 0.001708106926145092 1201 36-37 -0.02093746242316996 1201 38-39 -0.0051863011063844056 1201 40-41 0.04178043874468074 1201 42-43 -0.15476739423134944 1201 44-45 -0.014945326284326654 1201 46-47 -0.09191259840426369 1201 48-49 0.0840032648704252 1201 50-51 9.744419775259416E-4 1201 52-53 -0.017832315220985606 1201 54-55 0.023140608682105324 1201 56-57 -0.06753797553730578 1201 58-59 0.19805420946735808 1201 60-61 0.024129415732474513 1201 62-63 0.06287752501844324 1201 64-65 0.19363983856703726 1201 66-67 -0.010283786852454568 1201 68-69 -0.0011044658849854727 1201 70-71 0.2025401003711309 1201 72-73 0.20107590927241148 1201 74-75 -0.050807159428970294 1201 76-77 -0.26564945907394844 1201 78-79 -0.03754758057427665 1201 80-81 0.17410280197364614 1201 82-83 -0.08219515976634995 1201 84-85 -0.20534258524784832 1201 86-87 -0.07082771652966358 1201 88-89 0.5262069196512513 1201 90-91 0.4062937351999807 1202 1 0.10920187801293224 1202 2 -0.038253319997721746 1202 3 -0.05196754490798838 1202 4 -0.10172189533456333 1202 5 0.12544465742931266 1202 6 0.2687756908892993 1202 7 0.12033663978451159 1202 8 -0.027289422053890178 1202 9 -0.015649200219193915 1202 10-11 0.058079158350359705 1202 12-13 -0.027703783180868413 1202 14-15 -0.17382461438798202 1202 16-17 -0.03345389291825995 1202 18-19 -0.17528281602012186 1202 20-21 -0.03271350954453567 1202 22-23 0.017449995278383312 1202 24-25 -0.057274479817927215 1202 26-27 0.034171237304391866 1202 28-29 -0.0245921093263064 1202 30-31 0.05636096494809095 1202 32-33 -0.08478058913566144 1202 34-35 0.043313036356586565 1202 36-37 0.05227200049078107 1202 38-39 -0.054960598582098896 1202 40-41 -0.009017250589756998 1202 42-43 0.06555328843406727 1202 44-45 -0.008649080258649633 1202 46-47 -0.06964096154366217 1202 48-49 0.001957680345476831 1202 50-51 -0.13140593113347165 1202 52-53 -0.0926622786668645 1202 54-55 0.06463004787068627 1202 56-57 -0.01848077240963164 1202 58-59 0.029908692095183653 1202 60-61 -0.009560477434902737 1202 62-63 0.1400822537948514 1202 64-65 -0.040282616747397526 1202 66-67 -0.32991999328024235 1202 68-69 0.04471209685438993 1202 70-71 0.15875863889726816 1202 72-73 -0.018316642223702218 1202 74-75 0.13767658265948057 1202 76-77 -0.3388656531312222 1202 78-79 -0.2751423159473845 1202 80-81 -0.3199850175582384 1202 82-83 0.15914714738496372 1202 84-85 0.03521156629037847 1202 86-87 -0.6100886198299733 1202 88-89 -0.10236450892018212 1202 90-91 0.4062937351999807 1203 1 0.23351632568238045 1203 2 0.14147172589744628 1203 3 0.13468382576539284 1203 4 0.21729732097579557 1203 5 -0.013769007758071439 1203 6 0.22349725062058923 1203 7 0.07204787903311427 1203 8 -0.06492274086753014 1203 9 -0.03640517306018154 1203 10-11 -0.08813271128292399 1203 12-13 -0.11442803983727856 1203 14-15 0.014116277523683607 1203 16-17 0.018009073958147326 1203 18-19 0.02224251078077799 1203 20-21 0.060914070396435704 1203 22-23 0.02468252744094457 1203 24-25 0.05852222723074618 1203 26-27 0.0904515795091072 1203 28-29 -0.057810578950679314 1203 30-31 0.0279526826852603 1203 32-33 0.008971978948643766 1203 34-35 -0.0997529331178626 1203 36-37 0.010065534838396673 1203 38-39 -0.07843556094668713 1203 40-41 -0.0568717448713727 1203 42-43 -0.08166251297020466 1203 44-45 -0.032401690988557164 1203 46-47 -0.16082298837064002 1203 48-49 -0.12026325909260294 1203 50-51 -0.0923546614579962 1203 52-53 -0.21650209760136363 1203 54-55 -0.009796720998174635 1203 56-57 0.08648411783941867 1203 58-59 -0.022852637072034554 1203 60-61 -0.14490545919892384 1203 62-63 -0.1890102131136473 1203 64-65 -0.26113824724080814 1203 66-67 -0.17801099874442627 1203 68-69 0.023613614258302107 1203 70-71 -0.416308969591185 1203 72-73 -0.5016007455468809 1203 74-75 -0.2421417831450725 1203 76-77 -0.46493924945692555 1203 78-79 -0.8661982361859017 1203 80-81 -0.49893272802783173 1203 82-83 -0.6165708813563313 1203 84-85 -0.7545456344508921 1203 86-87 -0.6888044839116887 1203 88-89 -0.5639891587801245 1203 90-91 -1.4603729314666865 1204 1 0.2392468737092699 1204 2 0.0310860414760441 1204 3 0.012236596752380535 1204 4 0.014993203476038275 1204 5 -0.09390536768407998 1204 6 0.012703842272692611 1204 7 -0.10951724403808072 1204 8 0.044959341554402954 1204 9 -0.3329907791742386 1204 10-11 0.011663495929923329 1204 12-13 0.003470168916820171 1204 14-15 -0.114834888301111 1204 16-17 -0.12337303682599554 1204 18-19 -0.09190635341685294 1204 20-21 -0.1434708641560576 1204 22-23 -0.1220026230793394 1204 24-25 -0.0062362859258584535 1204 26-27 -0.14145022363507564 1204 28-29 -0.08577847570673924 1204 30-31 -0.07914436999787 1204 32-33 -0.13084743489179118 1204 34-35 -0.0918611926438615 1204 36-37 -0.11121962043423395 1204 38-39 -0.08508931659146413 1204 40-41 -0.1125185840617462 1204 42-43 -0.1417332583757016 1204 44-45 -0.04023744783692962 1204 46-47 -0.3103842989593275 1204 48-49 -0.15397465514467257 1204 50-51 -0.10297244894727697 1204 52-53 -0.09140535106399739 1204 54-55 0.03237514028440813 1204 56-57 -0.03364212115120324 1204 58-59 -0.1365918754325719 1204 60-61 -0.06314523858296184 1204 62-63 -0.3238480168348232 1204 64-65 -0.2546260157599889 1204 66-67 -0.2780791800904723 1204 68-69 -0.06712507253808297 1204 70-71 -0.33511182012286156 1204 72-73 -0.21781001372627884 1204 74-75 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56-57 0.19319624073546038 2401 58-59 0.25224589584137647 2401 60-61 0.24145309972016804 2401 62-63 0.18123401498336733 2401 64-65 0.07083056732221138 2401 66-67 0.14531818673698638 2401 68-69 0.11554659600381711 2401 70-71 0.2635198852732401 2401 72-73 0.17810065429769395 2401 74-75 0.06013789613562892 2401 76-77 -0.09608739200731264 2401 78-79 -0.14729333929832222 2401 80-81 0.12331052077352922 2401 82-83 0.15370814194594828 2401 84-85 0.3855424174018651 2401 86-87 0.552748121487781 2401 88-89 0.5262069196512513 2401 90-91 0.04265737156362093 2402 1 0.31120765882864987 2402 2 0.20352139896668575 2402 3 0.23925065313404303 2402 4 0.17874244847453724 2402 5 0.09573621718219982 2402 6 0.17801773799825327 2402 7 0.05160726649495473 2402 8 0.16765129683366098 2402 9 0.17311620710630393 2402 10-11 0.07751277503189868 2402 12-13 0.16515430505128847 2402 14-15 0.180025349108341 2402 16-17 0.03992916510763678 2402 18-19 0.08754363569469348 2402 20-21 0.06265543372747118 2402 22-23 0.08400451810491916 2402 24-25 0.06380073482795012 2402 26-27 0.03130644605843713 2402 28-29 0.12055162122107532 2402 30-31 0.06727620384435795 2402 32-33 0.05864233376573935 2402 34-35 0.0067365498120253164 2402 36-37 0.07638812955061525 2402 38-39 0.13945032087746512 2402 40-41 0.10973011239784825 2402 42-43 0.0919954878474627 2402 44-45 0.10228790462916493 2402 46-47 0.04026450731847575 2402 48-49 0.0854184561377167 2402 50-51 0.1566538669567592 2402 52-53 0.10179048672597446 2402 54-55 0.17617893616967706 2402 56-57 0.04477664826208638 2402 58-59 0.0481886413930539 2402 60-61 0.25769527924859403 2402 62-63 0.2605999680676092 2402 64-65 0.1809470785687992 2402 66-67 0.12241281408624616 2402 68-69 0.09164532520232882 2402 70-71 0.179746864753902 2402 72-73 0.10150350196735758 2402 74-75 0.306868328695046 2402 76-77 0.11746293756625192 2402 78-79 0.06084822725048156 2402 80-81 0.13107338060112284 2402 82-83 -0.014190625952814173 2402 84-85 -0.10136678120697695 2402 86-87 0.23760368786233244 2402 88-89 0.5262069196512513 2402 90-91 -0.7270395981333522 2403 1 -0.28309984708033653 2403 2 -0.10184535575554321 2403 3 -0.04062699598109276 2403 4 -0.07372145205577851 2403 5 0.04260693440467378 2403 6 0.025821976582477646 2403 7 0.030960554674990703 2403 8 0.03390152028292448 2403 9 0.025464907128153413 2403 10-11 0.1303520703225729 2403 12-13 0.0975594135979776 2403 14-15 0.09907812285851492 2403 16-17 0.1015614371397362 2403 18-19 0.08590997331163663 2403 20-21 0.0887910525329687 2403 22-23 0.07563957083894479 2403 24-25 0.06449789652577209 2403 26-27 0.1564330673069776 2403 28-29 0.09173163194664369 2403 30-31 0.0663347376199539 2403 32-33 0.06767643315878757 2403 34-35 0.17515009847064533 2403 36-37 0.09716117336331109 2403 38-39 0.1560960001976568 2403 40-41 0.09526110845250457 2403 42-43 0.07910336686138209 2403 44-45 0.1337864655032206 2403 46-47 0.02022219751725629 2403 48-49 0.013604000530463622 2403 50-51 0.16661200637800988 2403 52-53 0.057140327643224964 2403 54-55 0.02163348711258095 2403 56-57 0.18869023799913975 2403 58-59 0.0036255374075437885 2403 60-61 0.06694579631191289 2403 62-63 0.032539797621730315 2403 64-65 0.14813305095481866 2403 66-67 0.19702172562945464 2403 68-69 0.09305985316387222 2403 70-71 0.11203931495136032 2403 72-73 -0.07563774339616458 2403 74-75 0.34846953939517533 2403 76-77 0.3444892835761806 2403 78-79 0.3142936768483864 2403 80-81 0.22518726276776135 2403 82-83 0.271241588891165 2403 84-85 -0.0015101287692829146 2403 86-87 0.20978693840174856 2403 88-89 0.026206919651251326 2403 90-91 0.4062937351999807 2404 1 -0.404425192930141 2404 2 -0.31726809538005085 2404 3 -0.24867726772022536 2404 4 -0.2008135345656612 2404 5 -0.32919704286805285 2404 6 -0.20965696363491304 2404 7 -0.12325140184675121 2404 8 -0.05036848959849749 2404 9 -0.09384555482836987 2404 10-11 -0.04468807295805277 2404 12-13 -0.021700869257756494 2404 14-15 0.07044129603045235 2404 16-17 -0.03206164833457592 2404 18-19 0.02165756471479341 2404 20-21 0.02526045100134411 2404 22-23 -0.02942002649307085 2404 24-25 -0.026878246013758655 2404 26-27 0.02810194700064983 2404 28-29 -0.12001645352766843 2404 30-31 -0.06482571198478837 2404 32-33 0.008680141896626026 2404 34-35 -0.0068224172188138255 2404 36-37 -0.04975631421460491 2404 38-39 0.07575394996121076 2404 40-41 -0.018670419261766824 2404 42-43 -0.059054337514503175 2404 44-45 0.055309562069346896 2404 46-47 0.041133670255156574 2404 48-49 0.027559374120798452 2404 50-51 0.016127859508770825 2404 52-53 -0.030955502394803602 2404 54-55 0.10531794019949103 2404 56-57 0.10853480418425931 2404 58-59 0.1739216755194164 2404 60-61 0.0210030626240183 2404 62-63 0.018010683860261167 2404 64-65 0.1501889950329982 2404 66-67 0.12485688277860874 2404 68-69 -0.023683309607676506 2404 70-71 -0.0021078811227965843 2404 72-73 0.03687211350209907 2404 74-75 0.29804718227045157 2404 76-77 0.3638022719906857 2404 78-79 0.041011823566527994 2404 80-81 0.24419917580582506 2404 82-83 0.08709169828032515 2404 84-85 0.24158654452918427 2404 86-87 0.2990355892942347 2404 88-89 0.14525453869887173 2404 90-91 -0.7019313730251255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 11.0 31 299.0 32 1901.0 33 3565.0 34 4648.0 35 5773.0 36 8456.0 37 13137.0 38 35031.0 39 202021.0 40 200953.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.48326681252903 13.72472367068734 30.239327772067988 29.552681744715642 2 25.60251220158148 18.191811803479922 30.146839359506306 26.058836635432296 3 26.84234530562661 20.070703015807297 22.68276330252862 30.404188376037478 4 26.513939827026345 21.525026534536934 21.886736935024537 30.074296703412184 5 27.016677350539624 21.273867947330256 21.661639992013367 30.04781471011675 6 27.38805578032556 23.091037106316797 24.09314936054393 25.42775775281371 7 26.093800901648816 23.349761977322167 23.181201988251242 27.37523513277777 8 25.168612532708412 24.437835622484474 23.58221502958207 26.811336815225044 9 24.8052207358211 24.502149034773378 24.221776185121744 26.470854044283776 10-11 24.357128595298395 23.30888302735422 24.285353986485777 28.04863439086161 12-13 24.932586513099128 23.150831765781483 23.97734318351391 27.939238537605483 14-15 25.356718754925968 22.785548397944495 23.73648314925546 28.121249697874084 16-17 25.453399047909286 22.65292825691737 23.400729305688376 28.492943389484964 18-19 25.533265376895514 22.991309282358998 23.286079088683152 28.189346252062336 20-21 25.54997425361763 23.02630334492796 23.19044966844965 28.23327273300476 22-23 25.35861032587564 22.607740728675164 23.468615685326665 28.56503326012253 24-25 25.470423186456355 23.039649428850662 23.3258020786263 28.16412530606669 26-27 25.620908164230393 23.109007030338695 23.31581878750302 27.954266017927885 28-29 25.708656038840257 22.85721791948213 23.30457444907996 28.12955159259765 30-31 25.738296857233532 22.985302370158326 23.436761098885857 27.83963967372228 32-33 25.85119540111008 23.048208191126278 23.412559899334642 27.688036508428997 34-35 25.718570608977476 22.80872450571535 23.602308730656667 27.870396154650507 36-37 25.832578847643674 23.085395334944977 23.61695772702241 27.46506809038894 38-39 25.88687196982861 23.089997772877567 23.660361381207494 27.362768876086335 40-41 25.785664947355507 22.966554126024004 23.726364851168764 27.52141607545172 42-43 25.8268087489917 23.276503142345728 23.775005580762187 27.121682527900386 44-45 25.882406169545746 23.20001401108043 23.766149229682473 27.15143058969135 46-47 25.975708957146402 22.94440289593947 23.86058646365321 27.219301683260916 48-49 25.991011937637552 23.386085458778975 23.736441942724184 26.886460660859292 50-51 26.03881734083076 23.26482858697624 23.74297115907854 26.953382913114453 52-53 26.031288287968863 23.121895104261466 23.92281539437347 26.92400121339621 54-55 26.16440503621687 23.416303925337203 23.71072032160321 26.708570716842722 56-57 26.186321908273445 23.368777982617857 23.619680951126828 26.825219157981866 58-59 26.24106695328204 23.15202844106385 23.804405677878083 26.802498927776025 60-61 26.2408860070089 23.511464312497683 23.64833460223498 26.59931507825844 62-63 26.495431330553686 23.261877906903063 23.526429847795505 26.716260914747743 64-65 26.440461854501663 23.23687752355316 23.53722462279521 26.78543599914996 66-67 26.4520999721858 23.50419199745699 23.466841498788096 26.57686653156912 68-69 26.612895982467645 23.317046444578175 23.328722706737203 26.74133486621698 70-71 26.659404056832482 23.198412454864414 23.33496997780301 26.807213510500098 72-73 26.669022587389936 23.405483405483405 23.424330771269545 26.50116323585711 74-75 26.830101298506136 23.401116127842403 23.314366901413262 26.454415672238195 76-77 26.90002976022907 23.27008614379821 23.2017180499811 26.62816604599162 78-79 27.076260182932106 23.826378001981283 22.480836370980928 26.616525444105683 80-81 27.30352621213542 23.43735346525368 22.27797055237738 26.98114977023352 82-83 27.592449694307593 22.971448922656855 22.121149166023624 27.31495221701193 84-85 28.45264771789126 23.26335652789244 21.486417423438297 26.797578330778 86-87 28.527014536593565 22.065372657759852 21.099067305268466 28.30854550037812 88-89 27.866593164277838 21.628077912532156 21.79805218669607 28.707276736493938 90-91 26.07420773655126 23.333709259050412 23.09687605729108 27.495206947107253 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 3.5 11 5.0 12 5.0 13 7.5 14 12.5 15 18.5 16 29.0 17 49.0 18 78.0 19 109.5 20 147.0 21 206.0 22 284.5 23 406.5 24 557.5 25 699.0 26 914.0 27 1211.5 28 1540.0 29 1901.5 30 2369.0 31 2944.5 32 3596.5 33 4369.5 34 5348.5 35 6510.0 36 7816.5 37 9117.5 38 10584.5 39 12519.5 40 14546.0 41 16473.0 42 18454.5 43 20527.5 44 22594.5 45 24653.5 46 26597.0 47 28454.5 48 30080.5 49 31418.5 50 32679.0 51 33718.5 52 34290.5 53 34357.5 54 34385.0 55 34344.0 56 33921.5 57 33303.0 58 32511.0 59 31307.5 60 30055.0 61 28721.5 62 27323.5 63 25886.0 64 24005.0 65 21939.5 66 19814.0 67 17981.5 68 16120.5 69 13931.5 70 11986.5 71 10228.5 72 8513.5 73 6808.0 74 5271.5 75 4174.0 76 3261.5 77 2383.5 78 1774.0 79 1351.0 80 937.0 81 621.0 82 428.5 83 285.0 84 181.0 85 121.5 86 82.0 87 57.5 88 41.5 89 23.0 90 9.5 91 6.5 92 5.5 93 4.5 94 3.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.406981998549795E-4 2 0.007776458348658561 3 4.2034909992748977E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7668.0 32-33 16619.0 34-35 18216.0 36-37 19441.0 38-39 21223.0 40-41 21941.0 42-43 22425.0 44-45 22464.0 46-47 22424.0 48-49 22144.0 50-51 22097.0 52-53 21158.0 54-55 20358.0 56-57 19455.0 58-59 18438.0 60-61 17557.0 62-63 17040.0 64-65 15523.0 66-67 14746.0 68-69 13797.0 70-71 13111.0 72-73 11925.0 74-75 11207.0 76-77 10404.0 78-79 9493.0 80-81 9107.0 82-83 8318.0 84-85 7833.0 86-87 7105.0 88-89 6557.0 90-91 6001.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.76124936116064 #Duplication Level Percentage of deduplicated Percentage of total 1 99.7696704736858 99.53146974806198 2 0.2216818189567984 0.4423051043956967 3 0.008302919333891453 0.02484928818281841 4 3.4478802348586533E-4 0.001375859359508605 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.001891570949673704 0.0 0.0 0.0 0.0 2 0.002101745499637449 0.0 0.0 0.0 0.0 3 0.0023119200496011937 0.0 0.0 0.0 0.0 4 0.0023119200496011937 0.0 0.0 0.0 0.0 5 0.0023119200496011937 0.0 0.0 0.0 2.1017454996374488E-4 6 0.0023119200496011937 0.0 0.0 0.0 2.1017454996374488E-4 7 0.0027322691495286835 0.0 0.0 0.0 2.1017454996374488E-4 8 0.007145934698767327 0.0 0.0 0.0 2.1017454996374488E-4 9 0.007145934698767327 0.0 0.0 0.0 2.1017454996374488E-4 10-11 0.007145934698767327 0.0 0.0 0.0 3.152618249456173E-4 12-13 0.007145934698767327 0.0 0.0 0.0 6.305236498912347E-4 14-15 0.007145934698767327 0.0 0.0 0.0 6.305236498912347E-4 16-17 0.007145934698767327 1.0508727498187244E-4 0.0 0.0 6.305236498912347E-4 18-19 0.007356109248731071 2.1017454996374488E-4 0.0 0.0 7.356109248731072E-4 20-21 0.0074611965237129435 2.1017454996374488E-4 0.0 0.0 8.406981998549795E-4 22-23 0.007566283798694816 2.1017454996374488E-4 0.0 0.0 8.406981998549795E-4 24-25 0.007566283798694816 2.1017454996374488E-4 0.0 0.0 9.45785474836852E-4 26-27 0.007776458348658561 2.1017454996374488E-4 0.0 0.0 0.0010508727498187245 28-29 0.008301894723567923 2.1017454996374488E-4 0.0 0.0 0.001155960024800597 30-31 0.009247680198404775 2.1017454996374488E-4 0.0 0.0 0.0012610472997824694 32-33 0.009562942023350392 2.1017454996374488E-4 0.0 0.0 0.0012610472997824694 34-35 0.009668029298332265 2.1017454996374488E-4 0.0 0.0 0.0012610472997824694 36-37 0.009668029298332265 2.1017454996374488E-4 0.0 0.0 0.0012610472997824694 38-39 0.009983291123277883 2.1017454996374488E-4 0.0 0.0 0.0013661345747643418 40-41 0.010613814773169116 2.1017454996374488E-4 0.0 0.0 0.0014712218497462141 42-43 0.010929076598114734 2.1017454996374488E-4 0.0 0.0 0.0014712218497462141 44-45 0.01113925114807848 2.1017454996374488E-4 0.0 0.0 0.001681396399709959 46-47 0.01113925114807848 2.1017454996374488E-4 0.0 0.0 0.001681396399709959 48-49 0.011349425698042225 2.1017454996374488E-4 0.0 0.0 0.001681396399709959 50-51 0.011349425698042225 2.1017454996374488E-4 0.0 0.0 0.001681396399709959 52-53 0.011454512973024097 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 54-55 0.01155960024800597 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 56-57 0.01155960024800597 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 58-59 0.011769774797969713 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 60-61 0.012190123897897203 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 62-63 0.012400298447860949 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 64-65 0.012400298447860949 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 66-67 0.013030822097752184 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 68-69 0.013030822097752184 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 70-71 0.013135909372734057 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 72-73 0.0133460839226978 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 74-75 0.013451171197679673 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 76-77 0.013451171197679673 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 78-79 0.013451171197679673 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 80 0.013451171197679673 2.1017454996374488E-4 0.0 0.0 0.001891570949673704 >>END_MODULE