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"CACGA":42556, "CACGT":306721, "CACGC":40822, "CACGG":40973, "CAGAA":74286, "CAGAT":190145, "CAGAC":44939, "CAGAG":70817, "CAGTA":25294, "CAGTT":44623, "CAGTC":110513, "CAGTG":28212, "CAGCA":88178, "CAGCT":53587, "CAGCC":96023, "CAGCG":52691, "CAGGA":87861, "CAGGT":48179, "CAGGC":69438, "CAGGG":53190, "CTAAA":28836, "CTAAT":19035, "CTAAC":21623, "CTAAG":23226, "CTATA":18656, "CTATT":32999, "CTATC":36661, "CTATG":29881, "CTACA":61498, "CTACT":25818, "CTACC":46292, "CTACG":28945, "CTAGA":60275, "CTAGT":13564, "CTAGC":20961, "CTAGG":21393, "CTTAA":23043, "CTTAT":30106, "CTTAC":19673, "CTTAG":31032, "CTTTA":38945, "CTTTT":84256, "CTTTC":72716, "CTTTG":61243, "CTTCA":80508, "CTTCT":101385, "CTTCC":85719, "CTTCG":65330, "CTTGA":55940, "CTTGT":54862, "CTTGC":52426, "CTTGG":68373, "CTCAA":57360, "CTCAT":42843, "CTCAC":39137, "CTCAG":51146, "CTCTA":30895, "CTCTT":63550, "CTCTC":50497, "CTCTG":47902, "CTCCA":198788, "CTCCT":112497, "CTCCC":74607, "CTCCG":62607, "CTCGA":51257, "CTCGT":41233, "CTCGC":48362, "CTCGG":51165, "CTGAA":246629, "CTGAT":44235, "CTGAC":35372, "CTGAG":49395, "CTGTA":22286, "CTGTT":64121, "CTGTC":36394, "CTGTG":38546, "CTGCA":56143, "CTGCT":91245, "CTGCC":74769, "CTGCG":49221, "CTGGA":77510, "CTGGT":69451, "CTGGC":72438, "CTGGG":75542, "CCAAA":98196, "CCAAT":47940, "CCAAC":101062, "CCAAG":90959, "CCATA":41743, "CCATT":47329, "CCATC":72485, "CCATG":49269, "CCACA":80592, "CCACT":38351, "CCACC":130202, "CCACG":53050, "CCAGA":126507, "CCAGT":137937, "CCAGC":111092, "CCAGG":72463, "CCTAA":29764, "CCTAT":29643, "CCTAC":36399, "CCTAG":37477, "CCTTA":29504, "CCTTT":68178, "CCTTC":89823, "CCTTG":64986, "CCTCA":69572, "CCTCT":60656, "CCTCC":125772, "CCTCG":62381, "CCTGA":56967, "CCTGT":54016, "CCTGC":98365, "CCTGG":73414, "CCCAA":101895, "CCCAT":54888, "CCCAC":89423, "CCCAG":92042, "CCCTA":36517, "CCCTT":54037, "CCCTC":69849, "CCCTG":60968, "CCCCA":100185, "CCCCT":61703, "CCCCC":71977, "CCCCG":68169, "CCCGA":55807, "CCCGT":38828, "CCCGC":80382, "CCCGG":61813, 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"CGGAC":31802, "CGGAG":69517, "CGGTA":31109, "CGGTT":49281, "CGGTC":47166, "CGGTG":58654, "CGGCA":64190, "CGGCT":67352, "CGGCC":67906, "CGGCG":87308, "CGGGA":50394, "CGGGT":44100, "CGGGC":54328, "CGGGG":59082, "GAAAA":98290, "GAAAT":60640, "GAAAC":61468, "GAAAG":73866, "GAATA":39006, "GAATT":44232, "GAATC":44116, "GAATG":38917, "GAACA":58988, "GAACT":202649, "GAACC":66262, "GAACG":39677, "GAAGA":561554, "GAAGT":44378, "GAAGC":80817, "GAAGG":76756, "GATAA":43409, "GATAT":34684, "GATAC":26553, "GATAG":47101, "GATTA":32423, "GATTT":61920, "GATTC":47386, "GATTG":48456, "GATCA":48869, "GATCT":46202, "GATCC":46042, "GATCG":560409, "GATGA":74746, "GATGT":42340, "GATGC":46544, "GATGG":66428, "GACAA":55004, "GACAT":34414, "GACAC":29517, "GACAG":45149, "GACTA":22574, "GACTT":40954, "GACTC":27935, "GACTG":31669, "GACCA":58920, "GACCT":44057, "GACCC":53352, "GACCG":47104, "GACGA":59091, "GACGT":28313, "GACGC":38415, "GACGG":45693, "GAGAA":82866, "GAGAT":171962, "GAGAC":49624, "GAGAG":53498, "GAGTA":22432, "GAGTT":41160, "GAGTC":25985, "GAGTG":24224, "GAGCA":443267, "GAGCT":39439, "GAGCC":52877, "GAGCG":39728, "GAGGA":101988, "GAGGT":52284, "GAGGC":65930, "GAGGG":53629, "GTAAA":25989, "GTAAT":20924, "GTAAC":22380, "GTAAG":22518, "GTATA":15354, "GTATT":28640, "GTATC":24334, "GTATG":22429, "GTACA":19846, "GTACT":18189, "GTACC":22157, "GTACG":18717, "GTAGA":74773, "GTAGT":22894, "GTAGC":28009, "GTAGG":29006, "GTTAA":21196, "GTTAT":26764, "GTTAC":23689, "GTTAG":29049, "GTTTA":30249, "GTTTT":76436, "GTTTC":60104, "GTTTG":65895, "GTTCA":45782, "GTTCT":54846, "GTTCC":64337, "GTTCG":43119, "GTTGA":58491, "GTTGT":58390, "GTTGC":51494, "GTTGG":82016, "GTCAA":44321, "GTCAT":35305, "GTCAC":86142, "GTCAG":40158, "GTCTA":27023, "GTCTT":50665, "GTCTC":48431, "GTCTG":266220, "GTCCA":44251, "GTCCT":43193, "GTCCC":37247, "GTCCG":30904, "GTCGA":43909, "GTCGT":39141, "GTCGC":40780, "GTCGG":44452, "GTGAA":50757, "GTGAT":43191, "GTGAC":26515, "GTGAG":38571, "GTGTA":17960, "GTGTT":42630, "GTGTC":24486, "GTGTG":31844, "GTGCA":30356, "GTGCT":41941, "GTGCC":39474, "GTGCG":31412, "GTGGA":57439, "GTGGT":66463, "GTGGC":55364, "GTGGG":65511, "GCAAA":58543, "GCAAT":38115, "GCAAC":56033, "GCAAG":59409, "GCATA":29938, "GCATT":38598, "GCATC":54841, "GCATG":35049, "GCACA":372162, "GCACT":25817, "GCACC":62815, "GCACG":33029, "GCAGA":104503, "GCAGT":32415, "GCAGC":67440, "GCAGG":84602, "GCTAA":23138, "GCTAT":31729, "GCTAC":30335, "GCTAG":31525, "GCTTA":29080, "GCTTT":63657, "GCTTC":86757, "GCTTG":62763, "GCTCA":46500, "GCTCT":42980, "GCTCC":66101, "GCTCG":48077, "GCTGA":59175, "GCTGT":48500, "GCTGC":70127, "GCTGG":98584, "GCCAA":81412, "GCCAT":52030, "GCCAC":63461, "GCCAG":88970, "GCCTA":36152, "GCCTT":61988, "GCCTC":70095, "GCCTG":72414, "GCCCA":77945, "GCCCT":56221, "GCCCC":65178, "GCCCG":57580, "GCCGA":71665, "GCCGT":52140, "GCCGC":86856, "GCCGG":76263, "GCGAA":52627, "GCGAT":46311, "GCGAC":42944, "GCGAG":51162, "GCGTA":22334, "GCGTT":37069, "GCGTC":35802, "GCGTG":34058, "GCGCA":42592, "GCGCT":41915, "GCGCC":63038, "GCGCG":44520, "GCGGA":60805, "GCGGT":57545, "GCGGC":80572, "GCGGG":65865, "GGAAA":75809, "GGAAT":50125, "GGAAC":58862, "GGAAG":532054, "GGATA":46017, "GGATT":54505, "GGATC":42734, "GGATG":61778, "GGACA":43244, "GGACT":31384, "GGACC":37561, "GGACG":41560, "GGAGA":137895, "GGAGT":35555, "GGAGC":57485, "GGAGG":104619, "GGTAA":27679, "GGTAT":28486, "GGTAC":22564, "GGTAG":51232, "GGTTA":30394, "GGTTT":81412, "GGTTC":59553, "GGTTG":88703, "GGTCA":45560, "GGTCT":53341, "GGTCC":35897, "GGTCG":47301, "GGTGA":68475, "GGTGT":47485, "GGTGC":53252, "GGTGG":99077, "GGCAA":64038, "GGCAT":49095, "GGCAC":40252, "GGCAG":81207, "GGCTA":38771, "GGCTT":75862, "GGCTC":53251, "GGCTG":86999, "GGCCA":63597, "GGCCT":53913, "GGCCC":49239, "GGCCG":65627, "GGCGA":78268, "GGCGT":47909, "GGCGC":61200, "GGCGG":104316, "GGGAA":57357, "GGGAT":50081, "GGGAC":35177, "GGGAG":73483, "GGGTA":31286, "GGGTT":77106, "GGGTC":43741, "GGGTG":66523, "GGGCA":59097, "GGGCT":58645, "GGGCC":46178, "GGGCG":64254, "GGGGA":62605, "GGGGT":66465, "GGGGC":55635, "GGGGG":121230 }, "overrepresented_sequences": { } }, "read2_before_filtering": { "total_reads": 1043246, "total_bases": 63638006, "q20_bases": 61918713, "q30_bases": 59144222, "total_cycles": 61, "quality_curves": { "A":[39.2869,39.0816,38.9783,39.0191,38.8962,39.1542,39.1033,38.9502,39.1286,39.1195,39.1445,39.1758,39.1629,39.1525,39.1383,39.1205,39.1526,39.1473,39.1654,39.1343,38.6016,39.098,39.1205,39.1622,38.6725,39.116,39.156,38.5904,39.039,38.7821,39.0998,39.1313,39.0638,39.0912,39.0793,39.043,39.0317,39.0251,38.7311,38.3956,38.8143,38.4786,38.6316,38.5039,38.3531,38.0859,37.6576,37.4538,37.6775,37.3951,37.177,36.908,36.717,36.318,36.1502,35.8463,35.6159,35.3078,35.0798,34.5633,34.2944], 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"CTTGA":44232, "CTTGT":45733, "CTTGC":42964, "CTTGG":57143, "CTCAA":44685, "CTCAT":35239, "CTCAC":31842, "CTCAG":35229, "CTCTA":23059, "CTCTT":52152, "CTCTC":41678, "CTCTG":39019, "CTCCA":67959, "CTCCT":95456, "CTCCC":62379, "CTCCG":51549, "CTCGA":39710, "CTCGT":33647, "CTCGC":38415, "CTCGG":41210, "CTGAA":43767, "CTGAT":36005, "CTGAC":28267, "CTGAG":33361, "CTGTA":15515, "CTGTT":52937, "CTGTC":29371, "CTGTG":30711, "CTGCA":42243, "CTGCT":75933, "CTGCC":61880, "CTGCG":39564, "CTGGA":59217, "CTGGT":57652, "CTGGC":57926, "CTGGG":56387, "CCAAA":78386, "CCAAT":39985, "CCAAC":84174, "CCAAG":60280, "CCATA":30980, "CCATT":39550, "CCATC":58473, "CCATG":39269, "CCACA":63141, "CCACT":31289, "CCACC":107898, "CCACG":42243, "CCAGA":56463, "CCAGT":40118, "CCAGC":90197, "CCAGG":56389, "CCTAA":22115, "CCTAT":24490, "CCTAC":28558, "CCTAG":18623, "CCTTA":20347, "CCTTT":57116, "CCTTC":73741, "CCTTG":53975, "CCTCA":54891, "CCTCT":50355, "CCTCC":104863, "CCTCG":50549, "CCTGA":43187, "CCTGT":44537, "CCTGC":81449, "CCTGG":59317, "CCCAA":80617, "CCCAT":44630, "CCCAC":73457, "CCCAG":61402, "CCCTA":26173, "CCCTT":44072, "CCCTC":56802, "CCCTG":49429, "CCCCA":78687, "CCCCT":50909, "CCCCC":56876, "CCCCG":55317, "CCCGA":41659, "CCCGT":30277, "CCCGC":64431, "CCCGG":49713, "CCGAA":45375, "CCGAT":36033, "CCGAC":39579, "CCGAG":41457, "CCGTA":20994, "CCGTT":32855, "CCGTC":40568, "CCGTG":30459, "CCGCA":47532, "CCGCT":47199, "CCGCC":99786, "CCGCG":43490, "CCGGA":45093, "CCGGT":42514, "CCGGC":63488, "CCGGG":46716, "CGAAA":45926, "CGAAT":26725, "CGAAC":35828, "CGAAG":47410, "CGATA":23799, "CGATT":35039, "CGATC":36171, "CGATG":45589, "CGACA":32469, "CGACT":27196, "CGACC":44673, "CGACG":40057, "CGAGA":37783, "CGAGT":22242, "CGAGC":36442, "CGAGG":44252, "CGTAA":18750, "CGTAT":16257, "CGTAC":14597, "CGTAG":19667, "CGTTA":14038, "CGTTT":33109, "CGTTC":33655, "CGTTG":34219, "CGTCA":29781, "CGTCT":33369, "CGTCC":35035, "CGTCG":38609, "CGTGA":25979, "CGTGT":17509, "CGTGC":24102, "CGTGG":36286, "CGCAA":33029, "CGCAT":24352, "CGCAC":29176, "CGCAG":37711, "CGCTA":16696, "CGCTT":39531, "CGCTC":35704, "CGCTG":44412, "CGCCA":61362, "CGCCT":60449, "CGCCC":61770, "CGCCG":70745, "CGCGA":29600, "CGCGT":21032, "CGCGC":35746, "CGCGG":42249, "CGGAA":42923, "CGGAT":33243, "CGGAC":24409, "CGGAG":44321, "CGGTA":21388, "CGGTT":40505, "CGGTC":37561, "CGGTG":46856, "CGGCA":46296, "CGGCT":54930, "CGGCC":53640, "CGGCG":67211, "CGGGA":37651, "CGGGT":35866, "CGGGC":42747, "CGGGG":46025, "GAAAA":79791, "GAAAT":49529, "GAAAC":49852, "GAAAG":52394, "GAATA":29653, "GAATT":36506, "GAATC":36114, "GAATG":31225, "GAACA":45404, "GAACT":35605, "GAACC":52685, "GAACG":31265, "GAAGA":73741, "GAAGT":34938, "GAAGC":63967, "GAAGG":60928, "GATAA":33291, "GATAT":28200, "GATAC":21146, "GATAG":27035, "GATTA":24364, "GATTT":51627, "GATTC":38944, "GATTG":38959, "GATCA":37684, "GATCT":37808, "GATCC":36896, "GATCG":32911, "GATGA":58315, "GATGT":34168, "GATGC":37338, "GATGG":53785, "GACAA":43360, "GACAT":26825, "GACAC":23033, "GACAG":28034, "GACTA":15992, "GACTT":33803, "GACTC":21976, "GACTG":24816, "GACCA":45212, "GACCT":34286, "GACCC":43214, "GACCG":37479, "GACGA":44751, "GACGT":22252, "GACGC":29684, "GACGG":36379, "GAGAA":63886, "GAGAT":42971, "GAGAC":39633, "GAGAG":35648, "GAGTA":16074, "GAGTT":33174, "GAGTC":20633, "GAGTG":19098, "GAGCA":43548, "GAGCT":30463, "GAGCC":41774, "GAGCG":30349, "GAGGA":77990, "GAGGT":40871, "GAGGC":51053, "GAGGG":42357, "GTAAA":20139, "GTAAT":16777, "GTAAC":17319, "GTAAG":14041, "GTATA":11171, "GTATT":23282, "GTATC":19806, "GTATG":18137, "GTACA":14650, "GTACT":14496, "GTACC":17478, "GTACG":14523, "GTAGA":25431, "GTAGT":18137, "GTAGC":22099, "GTAGG":22900, "GTTAA":16500, "GTTAT":21993, "GTTAC":19384, "GTTAG":14683, "GTTTA":21662, "GTTTT":64439, "GTTTC":49651, "GTTTG":55218, "GTTCA":35678, "GTTCT":45586, "GTTCC":53044, "GTTCG":34769, "GTTGA":45862, "GTTGT":48923, "GTTGC":42351, "GTTGG":68759, "GTCAA":33033, "GTCAT":28406, "GTCAC":22566, "GTCAG":25538, "GTCTA":18986, "GTCTT":41376, "GTCTC":40906, "GTCTG":33376, "GTCCA":33457, "GTCCT":34872, "GTCCC":30509, "GTCCG":24424, "GTCGA":32675, "GTCGT":30694, "GTCGC":31935, "GTCGG":34425, "GTGAA":37219, "GTGAT":33932, "GTGAC":20729, "GTGAG":26079, "GTGTA":12385, "GTGTT":34997, "GTGTC":19327, "GTGTG":25336, "GTGCA":22702, "GTGCT":33628, "GTGCC":31163, "GTGCG":24957, "GTGGA":42655, "GTGGT":54986, "GTGGC":44641, "GTGGG":52935, "GCAAA":43771, "GCAAT":30513, "GCAAC":45464, "GCAAG":37998, "GCATA":21424, "GCATT":31419, "GCATC":36294, "GCATG":27678, "GCACA":26938, "GCACT":20211, "GCACC":50351, "GCACG":25313, "GCAGA":44849, "GCAGT":25779, "GCAGC":54282, "GCAGG":67094, "GCTAA":17268, "GCTAT":24410, "GCTAC":23977, "GCTAG":15261, "GCTTA":20322, "GCTTT":52559, "GCTTC":71142, "GCTTG":51764, "GCTCA":35705, "GCTCT":34427, "GCTCC":54365, "GCTCG":37858, "GCTGA":45178, "GCTGT":38623, "GCTGC":56669, "GCTGG":80335, "GCCAA":60962, "GCCAT":42009, "GCCAC":51204, "GCCAG":56039, "GCCTA":25401, "GCCTT":50880, "GCCTC":58151, "GCCTG":58736, "GCCCA":59171, "GCCCT":44897, "GCCCC":53006, "GCCCG":44752, "GCCGA":52635, "GCCGT":41116, "GCCGC":68118, "GCCGG":59631, "GCGAA":38382, "GCGAT":36401, "GCGAC":33193, "GCGAG":32704, "GCGTA":14940, "GCGTT":29116, "GCGTC":27786, "GCGTG":26130, "GCGCA":30557, "GCGCT":32683, "GCGCC":49989, "GCGCG":34243, "GCGGA":44325, "GCGGT":46180, "GCGGC":63064, "GCGGG":52301, "GGAAA":59215, "GGAAT":40967, "GGAAC":47391, "GGAAG":58150, "GGATA":33058, "GGATT":45266, "GGATC":34398, "GGATG":49143, "GGACA":32161, "GGACT":24986, "GGACC":29613, "GGACG":31899, "GGAGA":71022, "GGAGT":28322, "GGAGC":45334, "GGAGG":81707, "GGTAA":20050, "GGTAT":23017, "GGTAC":17562, "GGTAG":28770, "GGTTA":21466, "GGTTT":69215, "GGTTC":49470, "GGTTG":74348, "GGTCA":34072, "GGTCT":43926, "GGTCC":28715, "GGTCG":37288, "GGTGA":50454, "GGTGT":39107, "GGTGC":42941, "GGTGG":81726, "GGCAA":48064, "GGCAT":39238, "GGCAC":31643, "GGCAG":50887, "GGCTA":27084, "GGCTT":62416, "GGCTC":43169, "GGCTG":70263, "GGCCA":46695, "GGCCT":43384, "GGCCC":38691, "GGCCG":50502, "GGCGA":57700, "GGCGT":37167, "GGCGC":47147, "GGCGG":82868, "GGGAA":43095, "GGGAT":40322, "GGGAC":27690, "GGGAG":47945, "GGGTA":21720, "GGGTT":65553, "GGGTC":36022, "GGGTG":52837, "GGGCA":43063, "GGGCT":48050, "GGGCC":36419, "GGGCG":50037, "GGGGA":46275, "GGGGT":53355, "GGGGC":44254, "GGGGG":41161 }, "overrepresented_sequences": { } }, "command": "/redser4/software/fastp -l 30 -q 25 -y -Y 30 --detect_adapter_for_pe -i /scratch3/cwanket/andy-lake/raw/AM-AL-13A_S83_R1_001.fastq.gz -I /scratch3/cwanket/andy-lake/raw/AM-AL-13A_S83_R2_001.fastq.gz -o AM-AL-13A.trim.R1.fastq.gz -O AM-AL-13A.trim.R2.fastq.gz -m --merged_out AM-AL-13A.trim.merged.fastq.gz " }