##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-12C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 470854 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 53 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.22651182744545 40.0 40.0 40.0 40.0 40.0 2 39.134712671019045 40.0 40.0 40.0 40.0 40.0 3 39.12621322108339 40.0 40.0 40.0 40.0 40.0 4 39.051731534615826 40.0 40.0 40.0 40.0 40.0 5 39.21355664388537 40.0 40.0 40.0 40.0 40.0 6 39.24567700391204 40.0 40.0 40.0 40.0 40.0 7 39.269455075246256 40.0 40.0 40.0 40.0 40.0 8 39.29748924295004 40.0 40.0 40.0 40.0 40.0 9 39.326925119039025 40.0 40.0 40.0 40.0 40.0 10-11 39.32413444507215 40.0 40.0 40.0 40.0 40.0 12-13 39.328704863928095 40.0 40.0 40.0 40.0 40.0 14-15 39.34211878841424 40.0 40.0 40.0 40.0 40.0 16-17 39.47274101950923 40.0 40.0 40.0 40.0 40.0 18-19 39.491281798604234 40.0 40.0 40.0 40.0 40.0 20-21 39.4979123040263 40.0 40.0 40.0 40.0 40.0 22-23 39.50121693773441 40.0 40.0 40.0 40.0 40.0 24-25 39.500906862849206 40.0 40.0 40.0 40.0 40.0 26-27 39.48977814779103 40.0 40.0 40.0 40.0 40.0 28-29 39.48969319576769 40.0 40.0 40.0 40.0 40.0 30-31 39.477858547447 40.0 40.0 40.0 40.0 40.0 32-33 39.482066724117615 40.0 40.0 40.0 40.0 40.0 34-35 39.47487842735126 40.0 40.0 40.0 40.0 40.0 36-37 39.478832705185695 40.0 40.0 40.0 40.0 40.0 38-39 39.46521284043581 40.0 40.0 40.0 40.0 40.0 40-41 39.446615039777775 40.0 40.0 40.0 40.0 40.0 42-43 39.44477485739699 40.0 40.0 40.0 40.0 40.0 44-45 39.4257523967232 40.0 40.0 40.0 40.0 40.0 46-47 39.420591447271335 40.0 40.0 40.0 40.0 40.0 48-49 39.42751805119627 40.0 40.0 40.0 40.0 40.0 50-51 39.43120027971983 40.0 40.0 40.0 40.0 40.0 52-53 39.410612671949515 40.0 40.0 40.0 40.0 40.0 54-55 39.40063602320694 40.0 40.0 40.0 40.0 40.0 56-57 39.39214266942983 40.0 40.0 40.0 40.0 40.0 58-59 39.37602325249611 40.0 40.0 40.0 40.0 40.0 60-61 39.355582140313345 40.0 40.0 40.0 40.0 40.0 62-63 39.34523955615117 40.0 40.0 40.0 40.0 40.0 64-65 39.3478591491441 40.0 40.0 40.0 40.0 40.0 66-67 39.345692473651525 40.0 40.0 40.0 40.0 40.0 68-69 39.336641219801876 40.0 40.0 40.0 40.0 40.0 70-71 39.34558501129056 40.0 40.0 40.0 40.0 40.0 72-73 39.335339025146965 40.0 40.0 40.0 40.0 40.0 74-75 39.322948554184876 40.0 40.0 40.0 40.0 40.0 76-77 39.33319868940317 40.0 40.0 40.0 40.0 40.0 78-79 39.30763540551904 40.0 40.0 40.0 40.0 40.0 80-81 39.31188230215528 40.0 40.0 40.0 40.0 40.0 82-83 39.29009581424317 40.0 40.0 40.0 40.0 40.0 84-85 39.287761288121985 40.0 40.0 40.0 40.0 40.0 86-87 39.28676863789947 40.0 40.0 40.0 40.0 40.0 88-89 39.36397105017356 40.0 40.0 40.0 40.0 40.0 90-91 39.51594499779813 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13956903770547058 1101 2 0.23304614112803534 1101 3 0.14347357776288305 1101 4 0.12913536772992273 1101 5 0.029003586873919573 1101 6 0.07887411114226239 1101 7 -0.048291487309185754 1101 8 -0.02477553850656733 1101 9 0.03235232002656829 1101 10-11 0.002719868354724042 1101 12-13 0.12315307504937323 1101 14-15 0.025271845724034847 1101 16-17 0.07890195115814436 1101 18-19 1.6726376180997704E-4 1101 20-21 -0.01088813237323194 1101 22-23 -0.025719402246117795 1101 24-25 0.07126770038011898 1101 26-27 0.04761841267472988 1101 28-29 0.0991002796624798 1101 30-31 0.1470614279935205 1101 32-33 0.020318745869239763 1101 34-35 0.008717767153150646 1101 36-37 -0.05838770386124281 1101 38-39 -0.0324029207991785 1101 40-41 0.088768317198614 1101 42-43 -0.04768763186245906 1101 44-45 0.07727144331233404 1101 46-47 0.07317381031988646 1101 48-49 0.17697383066184358 1101 50-51 0.09190366317742615 1101 52-53 0.020729650844629077 1101 54-55 0.09589447632952641 1101 56-57 0.006603555051015064 1101 58-59 0.19567020745699182 1101 60-61 0.09414002585990033 1101 62-63 -0.09708636139805549 1101 64-65 0.1731457397411731 1101 66-67 -0.030205202231591954 1101 68-69 0.1876558911017412 1101 70-71 -0.002781006766866767 1101 72-73 0.3283776540064878 1101 74-75 0.005188073050739206 1101 76-77 0.15750310789240984 1101 78-79 -0.1558259787606957 1101 80-81 0.07938719749050449 1101 82-83 -0.40716379430482874 1101 84-85 -0.7921155146509591 1101 86-87 -1.1808201646071197 1101 88-89 -1.7267651752958173 1101 90-91 0.4860349007717417 1102 1 0.24130557097029026 1102 2 0.12068578638892546 1102 3 0.27441506783230096 1102 4 0.3017041064608108 1102 5 -0.06125026355023522 1102 6 -0.012913944628664353 1102 7 0.16485294546151152 1102 8 0.062281439053847976 1102 9 0.04607942049774749 1102 10-11 0.01475310335332125 1102 12-13 -0.024259100411597956 1102 14-15 -0.11084483462997241 1102 16-17 0.0428517958565493 1102 18-19 -0.05923547890552072 1102 20-21 -0.01515307642336694 1102 22-23 0.03104252523698392 1102 24-25 -0.01270067957427301 1102 26-27 0.008261274077810299 1102 28-29 -0.12192622483598825 1102 30-31 -0.09534301679757107 1102 32-33 0.04200865304724033 1102 34-35 -0.08384028368580942 1102 36-37 -0.1592876404126855 1102 38-39 -0.05981856804474006 1102 40-41 -0.038108253938162306 1102 42-43 -0.06670976633094483 1102 44-45 0.08392661967712911 1102 46-47 -0.17884167959400799 1102 48-49 -0.20855562270369887 1102 50-51 -0.07660127006030137 1102 52-53 -0.08237258904141243 1102 54-55 -0.039175034706559586 1102 56-57 -0.03920235213110601 1102 58-59 0.020533592495411312 1102 60-61 0.010586482433325273 1102 62-63 -0.020502280537982642 1102 64-65 -0.29735169948461504 1102 66-67 0.23662840142764452 1102 68-69 -0.14403274731679971 1102 70-71 -0.2853452204155573 1102 72-73 -0.320215959263237 1102 74-75 -0.2868579823237525 1102 76-77 -0.263575813186506 1102 78-79 -0.06358604720392691 1102 80-81 -0.07406828429683543 1102 82-83 -0.10649774549909097 1102 84-85 0.11352510054515363 1102 86-87 0.31713819853028014 1102 88-89 -0.12579899172093434 1102 90-91 0.4860349007717417 1103 1 0.17035810721029776 1103 2 0.00852716882244664 1103 3 0.13932111059842356 1103 4 0.053167382207689684 1103 5 -0.05585181403515094 1103 6 0.07181839719541472 1103 7 -0.0422358823485709 1103 8 -0.09546089707585992 1103 9 0.0775733651770949 1103 10-11 0.09357225977833394 1103 12-13 -0.005877469487984399 1103 14-15 0.021209131930135072 1103 16-17 -0.0035264453429419973 1103 18-19 -0.0392271391080925 1103 20-21 0.08991222957097023 1103 22-23 0.024388622346300792 1103 24-25 0.029895059955016734 1103 26-27 -0.049419634511728816 1103 28-29 0.019269235803307083 1103 30-31 0.03638453188487745 1103 32-33 0.1035120595834158 1103 34-35 -4.972164444012606E-4 1103 36-37 0.1447523677617255 1103 38-39 0.012951432842505994 1103 40-41 -0.10431857555666824 1103 42-43 -0.035602288441936025 1103 44-45 -0.013452350775288835 1103 46-47 0.0216072624258814 1103 48-49 -0.04504289006442974 1103 50-51 0.06149273194587579 1103 52-53 -0.031192688357279508 1103 54-55 -0.07097209554500239 1103 56-57 0.10267783425378241 1103 58-59 -0.15372122088461282 1103 60-61 -0.013156007327602026 1103 62-63 -0.27705377465153447 1103 64-65 -0.3774483470231189 1103 66-67 -0.29181767419655813 1103 68-69 -0.10666144406331313 1103 70-71 0.20577286028808572 1103 72-73 0.13116136860123362 1103 74-75 0.09571104622101245 1103 76-77 -0.23444632386593867 1103 78-79 -0.03390060583370058 1103 80-81 -0.2760649408389497 1103 82-83 0.04556133929404638 1103 84-85 0.4301801809927426 1103 86-87 0.6250806625703049 1103 88-89 -0.16164128562774494 1103 90-91 -1.0853936706568277 1104 1 0.34064139634909907 1104 2 0.09837752926323162 1104 3 0.3561319091646524 1104 4 -0.30699691483282976 1104 5 0.06999931925464864 1104 6 0.10263575165328831 1104 7 0.17967505346498314 1104 8 -0.029895318171973884 1104 9 -0.1509201122437247 1104 10-11 -0.030026951382673417 1104 12-13 -0.13175025587518974 1104 14-15 -0.059760127334058666 1104 16-17 -0.0155838893446969 1104 18-19 0.03453830121597434 1104 20-21 -0.08144871756881855 1104 22-23 -0.00667191305151249 1104 24-25 0.07485188701006251 1104 26-27 -0.028915816904095948 1104 28-29 0.05269162443975972 1104 30-31 -0.04247438319500674 1104 32-33 -0.0835960948876675 1104 34-35 -0.10189495858039521 1104 36-37 -0.052990000182688846 1104 38-39 0.12735932839024855 1104 40-41 0.11970916268934673 1104 42-43 -0.09205294171213296 1104 44-45 0.037967181886138235 1104 46-47 -0.0833018364965099 1104 48-49 0.10106910060282814 1104 50-51 0.022541414293137052 1104 52-53 0.0025069790909526546 1104 54-55 0.16119472260177758 1104 56-57 -0.14080770581246327 1104 58-59 -0.21114541084496352 1104 60-61 0.009067578306598989 1104 62-63 -0.10898709202251666 1104 64-65 -0.11032053939556619 1104 66-67 0.047692200049318956 1104 68-69 0.10491204097157691 1104 70-71 -0.14038482927542617 1104 72-73 0.1894487984056923 1104 74-75 -0.21536527797576355 1104 76-77 -0.11055144170005349 1104 78-79 0.4263615052436265 1104 80-81 -0.32567658422371437 1104 82-83 0.4439238916872412 1104 84-85 0.05612864466939271 1104 86-87 0.3887975567219826 1104 88-89 0.7630898971679514 1104 90-91 0.4860349007717417 1201 1 0.26929523522839105 1201 2 0.0641939136933587 1201 3 0.0751100573735144 1201 4 0.10134345865248662 1201 5 -0.17862627495519234 1201 6 -0.05908704709083423 1201 7 0.11679598856529338 1201 8 0.09026431464732099 1201 9 0.10334180662850656 1201 10-11 0.03683662938573207 1201 12-13 0.02794403340570284 1201 14-15 -0.19767355237851092 1201 16-17 -0.024897379957131704 1201 18-19 -0.11866854055083564 1201 20-21 0.053490505954954415 1201 22-23 0.05250319128983705 1201 24-25 -0.10067328731251024 1201 26-27 0.0476253459188527 1201 28-29 0.018227453559511275 1201 30-31 0.06071480565339016 1201 32-33 -0.06261704983055694 1201 34-35 -0.015320245406286404 1201 36-37 -0.07649955176206191 1201 38-39 -0.06811884155529668 1201 40-41 0.06529352799731214 1201 42-43 0.05061595452476553 1201 44-45 -0.037325929744092434 1201 46-47 0.09322953373422393 1201 48-49 -0.040781253690973074 1201 50-51 -0.0047276612335807044 1201 52-53 0.055104943627085845 1201 54-55 0.1401996543823003 1201 56-57 0.1418684489663562 1201 58-59 0.10887697737682345 1201 60-61 0.22177938246748852 1201 62-63 0.0633242598058672 1201 64-65 -0.058062586382035875 1201 66-67 -0.0763189719347892 1201 68-69 -0.02399499937412486 1201 70-71 -0.10190955985690664 1201 72-73 -0.1760861678166208 1201 74-75 0.22653988109816225 1201 76-77 -0.0072959890038646336 1201 78-79 -0.49429311791099195 1201 80-81 -0.39587127448920967 1201 82-83 -0.49374896177508276 1201 84-85 0.05807091243445228 1201 86-87 0.22937726686690496 1201 88-89 -0.11966872352170554 1201 90-91 0.4860349007717417 1202 1 0.2315849598278774 1202 2 0.1980032556495246 1202 3 0.07655936902873606 1202 4 0.13885652749948463 1202 5 0.08553659887643761 1202 6 0.0478489891200482 1202 7 0.1065193359430694 1202 8 0.139512738560029 1202 9 -0.04363655073480999 1202 10-11 0.09200594529811212 1202 12-13 -0.11900771672419808 1202 14-15 -0.03361022090246735 1202 16-17 -0.032733992762409514 1202 18-19 0.016920355781124385 1202 20-21 -0.0615654318404637 1202 22-23 -0.046733963676949486 1202 24-25 -8.523383420495634E-4 1202 26-27 0.04837660897987206 1202 28-29 0.024350247506831124 1202 30-31 0.026162965371923974 1202 32-33 0.16578128057501829 1202 34-35 0.02364646286203964 1202 36-37 0.03946099871822639 1202 38-39 0.037788619395783485 1202 40-41 0.040130983901548234 1202 42-43 -0.002439359176861444 1202 44-45 0.028404491842003665 1202 46-47 -0.018172753278811626 1202 48-49 -0.07788236075332833 1202 50-51 0.00835983080366276 1202 52-53 -0.13550761065145878 1202 54-55 -0.07286183199857277 1202 56-57 -0.041520715057501434 1202 58-59 0.1416727484700715 1202 60-61 -0.0022579833726936727 1202 62-63 0.05847738828710192 1202 64-65 0.01578511523402426 1202 66-67 -0.3568456110197715 1202 68-69 -0.00644387270874347 1202 70-71 -0.10500228515366672 1202 72-73 0.033655061026280464 1202 74-75 -0.1840674434963958 1202 76-77 -0.15187610938153995 1202 78-79 0.05290118585907777 1202 80-81 -0.02007634009799375 1202 82-83 0.11550941252220781 1202 84-85 0.5893727321303501 1202 86-87 0.24321253070217352 1202 88-89 0.5853121193901742 1202 90-91 0.12239853713538196 1203 1 0.37655443769552477 1203 2 0.1868929560274779 1203 3 0.024343090963895975 1203 4 0.06231687915827422 1203 5 0.15475458715447843 1203 6 0.019651772933272582 1203 7 0.05696271327939684 1203 8 -0.025169837349700686 1203 9 0.02222187282595911 1203 10-11 0.1084492496004188 1203 12-13 -0.09633826259259592 1203 14-15 0.13659555243646793 1203 16-17 0.0689400077366571 1203 18-19 0.14789621195279778 1203 20-21 -0.019035290093611934 1203 22-23 0.02359322871290459 1203 24-25 -0.03348941406006389 1203 26-27 0.07655556666275487 1203 28-29 0.07515757768753417 1203 30-31 -0.01693318999577542 1203 32-33 0.16676103478113902 1203 34-35 0.11705783827058269 1203 36-37 0.05360142522431488 1203 38-39 0.03596145680858598 1203 40-41 0.0024750424791406545 1203 42-43 0.12127305351931739 1203 44-45 0.014196075961400823 1203 46-47 -0.11191512356916888 1203 48-49 -0.0962369659241844 1203 50-51 -0.12342672476486882 1203 52-53 -0.013761922518284564 1203 54-55 -0.032716405058792475 1203 56-57 0.09352654829521612 1203 58-59 0.04939260708206916 1203 60-61 -0.10702761578209419 1203 62-63 0.13306805602185534 1203 64-65 0.03200813995336205 1203 66-67 0.22082435103391873 1203 68-69 0.29539195585108047 1203 70-71 0.18567243452867643 1203 72-73 0.0673786413579549 1203 74-75 0.1383195823365071 1203 76-77 0.10901497182466358 1203 78-79 0.011943441228787322 1203 80-81 -0.06356531503098495 1203 82-83 0.5443981300925174 1203 84-85 0.16116811036459922 1203 86-87 -0.13300183464262716 1203 88-89 0.3186454527235085 1203 90-91 0.4860349007717417 1204 1 0.22617805644458144 1204 2 0.05564898260384865 1204 3 -0.04685600143553614 1204 4 -0.038150276826037555 1204 5 -0.004122477291915061 1204 6 -0.17622204945352138 1204 7 -0.026955115406593677 1204 8 -0.07014185642340465 1204 9 -0.10869628021275446 1204 10-11 -0.08143065183157461 1204 12-13 -0.04818435795817777 1204 14-15 -0.18541625635099734 1204 16-17 -0.11054446222549075 1204 18-19 -0.16105049293171447 1204 20-21 -0.0985941703869031 1204 22-23 -0.06901901420820167 1204 24-25 -0.16864323210258902 1204 26-27 -0.13545810605214115 1204 28-29 -0.01951819433799784 1204 30-31 -0.12320200355460287 1204 32-33 -0.25875796841887677 1204 34-35 -0.031745129024322694 1204 36-37 -0.29205010370127127 1204 38-39 -0.09493579634101224 1204 40-41 -0.24606102959188547 1204 42-43 -0.24259006458511578 1204 44-45 -0.12070673007702482 1204 46-47 -0.18299921132248897 1204 48-49 -0.2264586666687265 1204 50-51 0.01982907640051934 1204 52-53 0.028116435004676532 1204 54-55 -0.16561414618805514 1204 56-57 -0.13229741014808383 1204 58-59 -0.09937130221142354 1204 60-61 -0.05009619188272296 1204 62-63 -0.45389439390274333 1204 64-65 -0.31770991708391705 1204 66-67 -0.1949873415960255 1204 68-69 -0.445484017807118 1204 70-71 -0.6209580459435671 1204 72-73 -0.5412436572867705 1204 74-75 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0.13955863006558644 2401 50-51 0.2288129703987707 2401 52-53 0.02735962810976389 2401 54-55 0.189894550220437 2401 56-57 0.17258397307907813 2401 58-59 0.16138382061834022 2401 60-61 0.12702677569845378 2401 62-63 0.34806374256068295 2401 64-65 0.2736156153036404 2401 66-67 0.1764666463028206 2401 68-69 0.28631923419131766 2401 70-71 0.32806348549080866 2401 72-73 0.31056825453850934 2401 74-75 0.27426098080562156 2401 76-77 0.18812022291260888 2401 78-79 0.40303786386443363 2401 80-81 0.2110884108598583 2401 82-83 -0.028034403640610606 2401 84-85 0.35242494096569743 2401 86-87 -0.2633910062349969 2401 88-89 0.622739019974965 2401 90-91 0.4860349007717417 2402 1 0.3529451641477195 2402 2 0.27684444435618616 2402 3 0.3152257848251949 2402 4 0.18273093908690186 2402 5 0.25172068882734067 2402 6 0.18417255593688964 2402 7 0.23808856392485467 2402 8 0.2510123500596393 2402 9 0.04688229763156926 2402 10-11 0.15049790177213396 2402 12-13 0.10149412821711223 2402 14-15 0.2162983160015841 2402 16-17 0.01775102296923592 2402 18-19 0.07371719621025363 2402 20-21 0.03851784313070539 2402 22-23 0.021181304910967924 2402 24-25 0.0995462665408624 2402 26-27 0.038599639561645915 2402 28-29 0.06938144948870928 2402 30-31 0.14000577428199534 2402 32-33 0.05553347191654723 2402 34-35 0.07060729314927983 2402 36-37 0.03482143586732889 2402 38-39 0.15044441594812952 2402 40-41 0.1406294355210349 2402 42-43 0.12317022888660034 2402 44-45 0.16132765034039664 2402 46-47 0.13894892015256488 2402 48-49 0.12211791617857415 2402 50-51 0.14318948897148687 2402 52-53 0.13366565757341675 2402 54-55 0.1184148232308857 2402 56-57 0.11974874313669659 2402 58-59 0.14061587441381818 2402 60-61 -0.0516004484328505 2402 62-63 0.10559081016400995 2402 64-65 0.14871992281592838 2402 66-67 0.28530767285710823 2402 68-69 0.23193969552251303 2402 70-71 0.2438340713938416 2402 72-73 0.24093595138538149 2402 74-75 0.32504057982583134 2402 76-77 0.34825112324712393 2402 78-79 0.08159694339051526 2402 80-81 0.46615041593979356 2402 82-83 0.27679584220625486 2402 84-85 -0.06163478805466838 2402 86-87 0.31840050062698566 2402 88-89 0.5007948152007415 2402 90-91 -0.18063176589492969 2403 1 -0.1743509011861093 2403 2 -0.032096722497328756 2403 3 0.013977419728036011 2403 4 0.0789515886882981 2403 5 0.1472990551701443 2403 6 0.033753709399768184 2403 7 0.10317307283118282 2403 8 0.07240991950408215 2403 9 0.06758230853092329 2403 10-11 0.12868508646943866 2403 12-13 0.07324534271636196 2403 14-15 0.02537961738424599 2403 16-17 0.00460757192290373 2403 18-19 0.009312729032870948 2403 20-21 0.054829891586109625 2403 22-23 0.09355641001186399 2403 24-25 -0.013547650842049563 2403 26-27 0.07175264700435235 2403 28-29 -0.05676590196868858 2403 30-31 0.0861430243754171 2403 32-33 -5.737384543991197E-4 2403 34-35 0.012947749125018504 2403 36-37 0.04847437470426996 2403 38-39 0.035919163396783915 2403 40-41 0.06646348968999405 2403 42-43 0.023082266417958408 2403 44-45 0.02786759538157213 2403 46-47 -0.00547481804942862 2403 48-49 0.15177374919630182 2403 50-51 0.011476155231626706 2403 52-53 0.06024763079798845 2403 54-55 -0.037543615667530617 2403 56-57 0.05046165971510419 2403 58-59 0.0647119147386519 2403 60-61 0.19188632250712345 2403 62-63 0.2095029938801929 2403 64-65 0.16062632999614834 2403 66-67 0.14479668317566308 2403 68-69 0.13484159329605916 2403 70-71 0.4152141113257315 2403 72-73 0.3014690643526592 2403 74-75 0.4109508272426652 2403 76-77 0.4175824801625936 2403 78-79 0.3179377838840409 2403 80-81 0.36630293231200284 2403 82-83 0.3208860455301945 2403 84-85 0.013469986010740342 2403 86-87 0.5371685746582173 2403 88-89 0.2868994209774769 2403 90-91 0.4860349007717417 2404 1 -0.3921479092906992 2404 2 -0.14331207833026838 2404 3 -0.3172515053784508 2404 4 -0.3088283006869972 2404 5 -0.1111512376599535 2404 6 0.0014462673742130505 2404 7 -0.04827499419437231 2404 8 -0.008755226319394183 2404 9 0.137860608045834 2404 10-11 0.025149588214588903 2404 12-13 0.015562989427195362 2404 14-15 0.1269079168991567 2404 16-17 0.08235359357458094 2404 18-19 0.008783083682658344 2404 20-21 0.06604949937124616 2404 22-23 0.02604973629558316 2404 24-25 0.05522906250981663 2404 26-27 -0.02318836178054795 2404 28-29 -0.009353861918995676 2404 30-31 -0.04335341507677981 2404 32-33 0.0901383122241981 2404 34-35 0.022994234450443685 2404 36-37 -0.0023140403884127636 2404 38-39 0.02443181781078607 2404 40-41 -0.04285788798257073 2404 42-43 0.038434774987081255 2404 44-45 0.05548491765772923 2404 46-47 0.15230091429716452 2404 48-49 0.014135111179491844 2404 50-51 -0.062368813432733816 2404 52-53 0.03628383952499803 2404 54-55 0.05604342144651753 2404 56-57 -0.006555071933163958 2404 58-59 0.10732174728359212 2404 60-61 -0.010374614214143207 2404 62-63 0.16981390002625574 2404 64-65 0.09240454256220687 2404 66-67 0.15481070469697755 2404 68-69 0.11121503522527121 2404 70-71 0.008548185779716277 2404 72-73 -0.02389208956206801 2404 74-75 0.10468296607141525 2404 76-77 0.24427994306152812 2404 78-79 -0.30067454010637107 2404 80-81 0.06297476881816522 2404 82-83 0.10352796115014229 2404 84-85 0.47309399711861744 2404 86-87 -0.04452509399197879 2404 88-89 -0.7869101028320458 2404 90-91 0.20032061505745702 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 277.0 32 1862.0 33 3517.0 34 4650.0 35 5670.0 36 8261.0 37 12560.0 38 32907.0 39 197145.0 40 203993.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.17426430610303 14.095419328530651 29.7165964387658 30.01371992660052 2 24.94902510513572 18.5705789898475 29.863429760842784 26.616966144173993 3 26.134917988569583 20.03954558680171 23.275190135266296 30.550346289362405 4 26.743109329006444 21.35821294923692 21.78106164543574 30.117616076320896 5 26.88816490886772 21.129904386497724 21.630484184057053 30.3514465205775 6 27.095872605945793 23.186805251734082 24.378894519320212 25.33842762299991 7 25.918012802269917 23.352674077314838 23.329524650953374 27.39978846946187 8 24.99904428973737 24.50844635492106 23.778496094330727 26.714013261010845 9 24.557930908519413 24.639060090813715 24.379319279436938 26.423689721229938 10-11 24.32027762321229 23.5112157908821 24.332170906480567 27.836335679425044 12-13 24.647980053264916 23.3030833336873 24.11596800706801 27.932968605979774 14-15 25.198044404422603 23.014777404460833 23.85898813644994 27.92819005466663 16-17 25.237016145132035 22.81683919006741 23.52470192458809 28.421442740212466 18-19 25.354037557289523 23.21675083996313 23.3881415470613 28.041070055686053 20-21 25.351064236472453 23.17841623942878 23.266129203532305 28.204390320566457 22-23 25.24200707650354 22.823422971876635 23.42711328777073 28.50745666384909 24-25 25.254749880005267 23.230343163698304 23.514189111699167 28.00071784459726 26-27 25.53923296818122 23.20230899599451 23.327931800515657 27.930526235308612 28-29 25.50047360753015 23.00437078160109 23.49125206539607 28.003903545472696 30-31 25.636110901678776 23.22578658361111 23.469601220772244 27.66850129393787 32-33 25.696035812441476 23.127237054043853 23.627967376397155 27.548759757117512 34-35 25.595560932871624 23.00415063150263 23.664237477913478 27.736050957712273 36-37 25.66824444157339 23.351616729895113 23.666975957529274 27.313162871002223 38-39 25.823240621402782 23.299529717338476 23.649044184925376 27.228185476333373 40-41 25.635891568385848 23.20078797229154 23.74141876430206 27.421901695020555 42-43 25.772938784234846 23.38465248584567 23.725836242758408 27.11657248716108 44-45 25.834639429939816 23.52765526041333 23.666616170738386 26.97108913890847 46-47 25.67194695270115 23.317879848116686 23.764549787773518 27.245623411408648 48-49 25.792812191397346 23.522828894451024 23.785115131168098 26.899243782983532 50-51 26.015724923267904 23.531237274848912 23.545229335251562 26.90780846663163 52-53 25.909531753113242 23.355316755246925 23.700585793536874 27.03456569810296 54-55 26.07535824220247 23.621878145014925 23.68377835513877 26.618985257643835 56-57 26.287262872628723 23.47987638794393 23.653290632652734 26.579570106774607 58-59 26.194775249708652 23.41959556451411 23.626414848974406 26.759214336802827 60-61 26.24670546892159 23.649242257851967 23.522402811333187 26.58164946189326 62-63 26.372277047614716 23.671532736065 23.31802264768748 26.638167568632802 64-65 26.50277445619315 23.563060097542625 23.34439532977737 26.589770116486854 66-67 26.393825112258767 23.788373024938487 23.32323410119955 26.494567761603193 68-69 26.65901301400283 23.467523652300702 23.113891379584857 26.759571954111607 70-71 26.60550892540489 23.625960515427355 23.18193639910155 26.586594160066202 72-73 26.800127586188253 23.71373119320117 22.94010477204781 26.546036448562766 74-75 26.76200459219327 23.758360786662674 22.769416991115104 26.71021763002895 76-77 26.937933648183783 23.483324587487612 22.80406390297942 26.774677861349193 78-79 27.097308917609887 23.457397670664008 22.61130824419177 26.83398516753434 80-81 27.475387812506558 23.663385041667542 22.238082243539957 26.623144902285944 82-83 27.74726365525365 23.165292428232988 21.909287028336223 27.178156888177135 84-85 28.39491154287303 22.719057384171563 21.443467380348267 27.44256369260714 86-87 29.108706852138074 22.097102622604943 21.38073158165894 27.413458943598044 88-89 28.63090281907934 21.363149756155583 20.895285674636217 29.110661750128862 90-91 26.824534161490682 22.82608695652174 21.875 28.474378881987576 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 1.5 9 2.0 10 4.0 11 6.5 12 11.0 13 12.5 14 14.0 15 26.5 16 42.5 17 56.5 18 74.5 19 104.5 20 146.5 21 202.5 22 264.5 23 375.5 24 511.5 25 644.0 26 830.5 27 1107.5 28 1476.0 29 1843.0 30 2285.0 31 2890.5 32 3610.5 33 4312.5 34 5244.5 35 6467.5 36 7822.0 37 9253.5 38 10773.0 39 12654.0 40 14793.5 41 16844.5 42 18855.0 43 20968.5 44 22895.0 45 24938.5 46 27067.0 47 28723.0 48 30065.5 49 31314.5 50 32421.5 51 33227.5 52 33601.5 53 33752.0 54 33744.5 55 33246.5 56 32543.5 57 31942.5 58 31179.0 59 30107.5 60 28810.0 61 27507.0 62 26217.0 63 24714.0 64 22926.0 65 21040.0 66 19108.0 67 17383.0 68 15615.0 69 13487.5 70 11439.5 71 9584.5 72 7922.5 73 6391.0 74 4989.5 75 3907.0 76 3055.0 77 2267.0 78 1665.0 79 1205.0 80 818.5 81 564.0 82 379.0 83 233.0 84 152.5 85 111.0 86 70.0 87 47.0 88 35.0 89 21.0 90 10.5 91 6.0 92 5.0 93 4.5 94 3.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012742803501722402 2 0.007220921984309361 3 0.0010619002918102 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7293.0 32-33 15897.0 34-35 17372.0 36-37 18722.0 38-39 20125.0 40-41 21069.0 42-43 21686.0 44-45 21492.0 46-47 21486.0 48-49 21696.0 50-51 21117.0 52-53 20439.0 54-55 19485.0 56-57 18567.0 58-59 17896.0 60-61 17444.0 62-63 16352.0 64-65 15546.0 66-67 14382.0 68-69 13668.0 70-71 13418.0 72-73 12579.0 74-75 11761.0 76-77 10863.0 78-79 10326.0 80-81 10031.0 82-83 9198.0 84-85 8578.0 86-87 7912.0 88-89 7485.0 90-91 6969.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.71038978038015 #Duplication Level Percentage of deduplicated Percentage of total 1 99.72397459874071 99.43516377689166 2 0.2629839189898186 0.5244445813689347 3 0.01165696925096118 0.03486962843013736 4 0.0013845130185134562 0.005522013309279496 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0016990404668963204 0.0 0.0 0.0 0.0 2 0.0016990404668963204 0.0 0.0 0.0 2.1238005836204005E-4 3 0.0016990404668963204 0.0 0.0 0.0 2.1238005836204005E-4 4 0.0019114205252583603 0.0 0.0 0.0 2.1238005836204005E-4 5 0.0021238005836204 0.0 0.0 0.0 2.1238005836204005E-4 6 0.0021238005836204 0.0 0.0 0.0 2.1238005836204005E-4 7 0.0023361806419824405 0.0 0.0 0.0 6.371401750861201E-4 8 0.008282822276119562 0.0 0.0 0.0 6.371401750861201E-4 9 0.0084952023344816 0.0 0.0 0.0 6.371401750861201E-4 10-11 0.00860139236366262 0.0 0.0 0.0 6.371401750861201E-4 12-13 0.008707582392843641 0.0 0.0 0.0 6.371401750861201E-4 14-15 0.008707582392843641 0.0 0.0 0.0 6.371401750861201E-4 16-17 0.008707582392843641 0.0 0.0 0.0 6.371401750861201E-4 18-19 0.008707582392843641 0.0 0.0 0.0 6.371401750861201E-4 20-21 0.008707582392843641 0.0 0.0 0.0 8.495202334481602E-4 22-23 0.008707582392843641 0.0 0.0 0.0 8.495202334481602E-4 24-25 0.008813772422024663 0.0 0.0 0.0 8.495202334481602E-4 26-27 0.008919962451205682 0.0 0.0 0.0 8.495202334481602E-4 28-29 0.008919962451205682 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 30-31 0.00923853253874874 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 32-33 0.009557102626291801 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 34-35 0.009557102626291801 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 36-37 0.009769482684653842 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 38-39 0.009769482684653842 0.0 0.0 2.1238005836204005E-4 8.495202334481602E-4 40-41 0.009769482684653842 1.0619002918102003E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 42-43 0.009769482684653842 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 44-45 0.009981862743015883 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 46-47 0.010194242801377922 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 48-49 0.010406622859739962 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 50-51 0.010512812888920982 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 52-53 0.010619002918102001 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 54-55 0.011043763034826083 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 56-57 0.011149953064007102 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 58-59 0.011256143093188122 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 60-61 0.011680903209912202 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 62-63 0.011893283268274242 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 64-65 0.011893283268274242 2.1238005836204005E-4 0.0 2.1238005836204005E-4 8.495202334481602E-4 66-67 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