##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-12B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 423184 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.27063877651329 40.0 40.0 40.0 40.0 40.0 2 39.16451945253129 40.0 40.0 40.0 40.0 40.0 3 39.15770445007373 40.0 40.0 40.0 40.0 40.0 4 39.097498960263145 40.0 40.0 40.0 40.0 40.0 5 39.21094370297554 40.0 40.0 40.0 40.0 40.0 6 39.27470792846611 40.0 40.0 40.0 40.0 40.0 7 39.30332904835721 40.0 40.0 40.0 40.0 40.0 8 39.313924912094976 40.0 40.0 40.0 40.0 40.0 9 39.336997618057396 40.0 40.0 40.0 40.0 40.0 10-11 39.322951718401455 40.0 40.0 40.0 40.0 40.0 12-13 39.32817875912133 40.0 40.0 40.0 40.0 40.0 14-15 39.3371583046618 40.0 40.0 40.0 40.0 40.0 16-17 39.47694619834398 40.0 40.0 40.0 40.0 40.0 18-19 39.48764603576695 40.0 40.0 40.0 40.0 40.0 20-21 39.50426292109343 40.0 40.0 40.0 40.0 40.0 22-23 39.503672161518395 40.0 40.0 40.0 40.0 40.0 24-25 39.49906423683315 40.0 40.0 40.0 40.0 40.0 26-27 39.491389088434346 40.0 40.0 40.0 40.0 40.0 28-29 39.49318972361904 40.0 40.0 40.0 40.0 40.0 30-31 39.490208049199225 40.0 40.0 40.0 40.0 40.0 32-33 39.48711483894145 40.0 40.0 40.0 40.0 40.0 34-35 39.474841545233204 40.0 40.0 40.0 40.0 40.0 36-37 39.47786904564479 40.0 40.0 40.0 40.0 40.0 38-39 39.46362958355371 40.0 40.0 40.0 40.0 40.0 40-41 39.449093240856406 40.0 40.0 40.0 40.0 40.0 42-43 39.44181621214755 40.0 40.0 40.0 40.0 40.0 44-45 39.43434741631296 40.0 40.0 40.0 40.0 40.0 46-47 39.423676525808446 40.0 40.0 40.0 40.0 40.0 48-49 39.421939296087686 40.0 40.0 40.0 40.0 40.0 50-51 39.424083350706894 40.0 40.0 40.0 40.0 40.0 52-53 39.42401847282917 40.0 40.0 40.0 40.0 40.0 54-55 39.40416984842973 40.0 40.0 40.0 40.0 40.0 56-57 39.39747970511409 40.0 40.0 40.0 40.0 40.0 58-59 39.38929882895567 40.0 40.0 40.0 40.0 40.0 60-61 39.35120818113695 40.0 40.0 40.0 40.0 40.0 62-63 39.360545338298095 40.0 40.0 40.0 40.0 40.0 64-65 39.34943236633647 40.0 40.0 40.0 40.0 40.0 66-67 39.3504139393826 40.0 40.0 40.0 40.0 40.0 68-69 39.34148247048883 40.0 40.0 40.0 40.0 40.0 70-71 39.349892502723016 40.0 40.0 40.0 40.0 40.0 72-73 39.33983559808281 40.0 40.0 40.0 40.0 40.0 74-75 39.33118490660232 40.0 40.0 40.0 40.0 40.0 76-77 39.3223031723075 40.0 40.0 40.0 40.0 40.0 78-79 39.31804957078393 40.0 40.0 40.0 40.0 40.0 80-81 39.32390357170378 40.0 40.0 40.0 40.0 40.0 82-83 39.28901528662904 40.0 40.0 40.0 40.0 40.0 84-85 39.31368238131219 40.0 40.0 40.0 40.0 40.0 86-87 39.33329672850293 40.0 40.0 40.0 40.0 40.0 88-89 39.31535208059785 40.0 40.0 40.0 40.0 40.0 90-91 39.40858553289059 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.222180426527828 1101 2 0.13676576300346 1101 3 0.1830296249052097 1101 4 0.18252444605343499 1101 5 0.05628315398584505 1101 6 0.06277385483733156 1101 7 0.03629599223846469 1101 8 0.10141843725283906 1101 9 0.008754219919161699 1101 10-11 0.001705224516626913 1101 12-13 0.04409248299987922 1101 14-15 -0.012343039760267516 1101 16-17 0.018903029808328142 1101 18-19 0.043735135728432795 1101 20-21 0.03153747210539137 1101 22-23 0.1155889378254571 1101 24-25 0.12760618331349605 1101 26-27 0.05389275235280877 1101 28-29 0.03627593631598103 1101 30-31 0.10909369956124237 1101 32-33 -0.03933734239753761 1101 34-35 0.046082344846809065 1101 36-37 -0.009339652728655778 1101 38-39 0.10917032552789863 1101 40-41 0.02136312813961183 1101 42-43 0.0183307156465915 1101 44-45 0.15678514555502687 1101 46-47 0.1337436725718817 1101 48-49 0.04890858426306721 1101 50-51 6.705697134989919E-4 1101 52-53 0.0823867682429622 1101 54-55 0.12492665654010437 1101 56-57 0.05612789721642031 1101 58-59 0.04642436185717713 1101 60-61 0.07885564949497592 1101 62-63 0.06590406972748042 1101 64-65 -0.040086823610955946 1101 66-67 0.08055384068818938 1101 68-69 -0.2064510972982987 1101 70-71 0.1773164596454535 1101 72-73 0.159588363136848 1101 74-75 0.3957070606432609 1101 76-77 0.17492874664719693 1101 78-79 0.14680878502753103 1101 80-81 0.39259948454449045 1101 82-83 0.35511608231858816 1101 84-85 -0.056094455242401864 1101 86-87 -0.0390438091979064 1101 88-89 0.056834070210570076 1101 90-91 -0.030851987667993797 1102 1 0.30480494703679994 1102 2 0.06956016967725276 1102 3 0.08145110610328032 1102 4 -0.23804764029255665 1102 5 0.06685239526998288 1102 6 0.10804631038732992 1102 7 0.2577726120433681 1102 8 -0.037165506787737 1102 9 0.24792254620524545 1102 10-11 0.02249706736456858 1102 12-13 0.05533211242239844 1102 14-15 0.0262974784988117 1102 16-17 0.022559097802030692 1102 18-19 -0.0016321552167539721 1102 20-21 0.07026301520154021 1102 22-23 0.1353931844890468 1102 24-25 -0.06795094193136464 1102 26-27 -0.1030467459352451 1102 28-29 -0.017911046381456686 1102 30-31 0.053886418819040216 1102 32-33 0.03651846841992068 1102 34-35 0.016781375542819887 1102 36-37 0.038434524898121936 1102 38-39 -0.04686883231337191 1102 40-41 0.054077647935173445 1102 42-43 0.07111262303600796 1102 44-45 0.023459295259335988 1102 46-47 0.04495601340749289 1102 48-49 -0.004814321621360307 1102 50-51 0.1152950093226579 1102 52-53 0.0797733969463792 1102 54-55 -0.051383582490551305 1102 56-57 -0.026177702203796116 1102 58-59 0.05413243172410631 1102 60-61 0.054878213219517136 1102 62-63 0.09950890059673156 1102 64-65 0.16941748370533105 1102 66-67 -0.12803726843964824 1102 68-69 0.1748861740444596 1102 70-71 0.01217594909923747 1102 72-73 0.2713316297739965 1102 74-75 0.15105631487165283 1102 76-77 0.059183711067838374 1102 78-79 0.2078546506179464 1102 80-81 0.25645443965802883 1102 82-83 0.07153786278531271 1102 84-85 0.5352657200086668 1102 86-87 0.1453889922627596 1102 88-89 0.6401674035438987 1102 90-91 0.4691480123320062 1103 1 0.2739502471666384 1103 2 0.1872967676298174 1103 3 0.09802213691354922 1103 4 0.3172415134987858 1103 5 0.0442289065331849 1103 6 0.07325537360573264 1103 7 0.19698144817117935 1103 8 0.03523360657160879 1103 9 0.06323207232642147 1103 10-11 0.10538677867976531 1103 12-13 0.1077011774180292 1103 14-15 -0.10843141976624793 1103 16-17 -0.04299603173011235 1103 18-19 -0.07108073647651025 1103 20-21 0.08000322685655448 1103 22-23 0.03433128913832206 1103 24-25 0.002317140156954167 1103 26-27 0.0528509448167398 1103 28-29 0.04237985995683857 1103 30-31 -0.01633558691746373 1103 32-33 -0.02764700435567846 1103 34-35 0.042250354859262984 1103 36-37 -0.09339291830366392 1103 38-39 0.0824836144637473 1103 40-41 -0.11608593103937181 1103 42-43 -0.007749382511960334 1103 44-45 0.08145777048887481 1103 46-47 -0.08547050510111376 1103 48-49 -0.01310958219072944 1103 50-51 -0.0438190656043389 1103 52-53 -0.04968991469119999 1103 54-55 0.015379780671558763 1103 56-57 0.06723437019547873 1103 58-59 -0.13224907772581673 1103 60-61 -0.08764139369048252 1103 62-63 -0.2122887581607671 1103 64-65 -0.11167339981249569 1103 66-67 -0.34055678201305994 1103 68-69 -0.18070207271917837 1103 70-71 -0.4556257243449551 1103 72-73 -0.1013941199328201 1103 74-75 -0.28642176424588683 1103 76-77 -0.3287808785802184 1103 78-79 -0.122532337618253 1103 80-81 -0.6527307830140359 1103 82-83 -0.344511633936456 1103 84-85 -0.3819808603210788 1103 86-87 -0.21898402484859503 1103 88-89 0.15127851465501152 1103 90-91 0.4691480123320062 1104 1 0.293257990705996 1104 2 0.18689287442906988 1104 3 0.14479791519102037 1104 4 0.11961739464538113 1104 5 0.0043967942212006506 1104 6 0.11774627034754559 1104 7 0.09869197708992772 1104 8 0.0694178082478416 1104 9 0.13207195917038206 1104 10-11 0.07343800222888319 1104 12-13 0.039781177945563684 1104 14-15 0.09706779222987194 1104 16-17 -0.01611158145348668 1104 18-19 -0.011545230142807839 1104 20-21 0.08751564039043558 1104 22-23 0.05201356861262951 1104 24-25 -0.052806863298393125 1104 26-27 0.014570112104351551 1104 28-29 0.02070151061064962 1104 30-31 0.0026968498279131836 1104 32-33 0.07789333095713147 1104 34-35 -0.03545323044042448 1104 36-37 0.04946257194637127 1104 38-39 -0.03219304435578607 1104 40-41 -0.013828902511647811 1104 42-43 0.0039438469800217035 1104 44-45 0.004994965766762505 1104 46-47 -0.053162071061620964 1104 48-49 0.0053961369833430695 1104 50-51 -0.018284488041537372 1104 52-53 -0.023378593426087946 1104 54-55 -0.07199015759798755 1104 56-57 0.05065166460666859 1104 58-59 -0.15923413410710197 1104 60-61 0.03665801916309874 1104 62-63 -0.17838603004632603 1104 64-65 0.027421582256309307 1104 66-67 -0.04475536718928197 1104 68-69 -0.12038901952416836 1104 70-71 0.02051300161087255 1104 72-73 -0.08177916982926092 1104 74-75 -0.2895094061768191 1104 76-77 -0.27919212203988764 1104 78-79 -0.31993187364277986 1104 80-81 0.045117651231450395 1104 82-83 0.27205058728231535 1104 84-85 -0.3131731973957699 1104 86-87 0.04792360934845874 1104 88-89 0.045852158065862625 1104 90-91 -0.06418532100132524 1201 1 0.26737367216429675 1201 2 0.3819697418779171 1201 3 0.24710705574685932 1201 4 0.11336451133677627 1201 5 0.06426363212003139 1201 6 0.2330661461626704 1201 7 0.06882401703855834 1201 8 0.016202841226657938 1201 9 0.1201210346413788 1201 10-11 0.07542047171256172 1201 12-13 0.06387005242656585 1201 14-15 -0.07936264054695386 1201 16-17 0.022590212743736515 1201 18-19 0.0507654066164136 1201 20-21 -0.07159000403682825 1201 22-23 -0.022004859625717188 1201 24-25 -0.02185415579369021 1201 26-27 -0.007551878344884244 1201 28-29 0.09409652077235364 1201 30-31 0.09934223715755763 1201 32-33 0.03538990184643609 1201 34-35 0.05426350875230668 1201 36-37 -3.4531805933113446E-4 1201 38-39 -0.05476109306286503 1201 40-41 -0.0274026327962531 1201 42-43 -0.13824943258154576 1201 44-45 -0.023058455687461787 1201 46-47 -0.03895826454581197 1201 48-49 -0.02230484055792914 1201 50-51 0.04731044783846272 1201 52-53 -0.10648693889606875 1201 54-55 0.0717570521827895 1201 56-57 0.14255259491726235 1201 58-59 -0.1853183737068278 1201 60-61 -0.33723003578287347 1201 62-63 0.1521026165149948 1201 64-65 0.16135503561998377 1201 66-67 -0.022931533354217493 1201 68-69 -0.022748779650115125 1201 70-71 -0.07864206499632331 1201 72-73 -0.22314899209445116 1201 74-75 0.014284249583354836 1201 76-77 -0.1900301132936164 1201 78-79 -0.25342251602041443 1201 80-81 -0.16770732874553573 1201 82-83 -0.41797802673057305 1201 84-85 -1.0173788278309104 1201 86-87 -0.40837007326101826 1201 88-89 -1.7114454996819077 1201 90-91 0.4691480123320062 1202 1 0.21352912931175183 1202 2 0.2616481315193724 1202 3 0.07603681198951762 1202 4 -0.03851109380728701 1202 5 0.042604406765136105 1202 6 0.06688258301370098 1202 7 0.10765643424574733 1202 8 -0.13111388548648506 1202 9 0.030376082946673932 1202 10-11 -0.01870124609224888 1202 12-13 -0.053164430541379204 1202 14-15 -0.10308277033232827 1202 16-17 -0.014196476059687768 1202 18-19 0.061701251480997144 1202 20-21 0.020710590559900766 1202 22-23 0.060133867469865265 1202 24-25 0.0688590264066491 1202 26-27 0.055395245342772625 1202 28-29 0.11070516443014355 1202 30-31 0.058193958270749135 1202 32-33 -0.03889508065537228 1202 34-35 0.00550820968080501 1202 36-37 0.022790257110713696 1202 38-39 0.013242057698022336 1202 40-41 0.026224642966724332 1202 42-43 -0.10211779838131463 1202 44-45 0.1654275403649521 1202 46-47 0.027261413041543392 1202 48-49 -0.06722035539014115 1202 50-51 0.17472724667441497 1202 52-53 0.059308421390724675 1202 54-55 0.05609925232894142 1202 56-57 0.05529454945786938 1202 58-59 0.31799880309548456 1202 60-61 -0.18705032310936787 1202 62-63 0.06932162199118608 1202 64-65 -0.009596761973654111 1202 66-67 -0.040036401293839674 1202 68-69 -0.20437354868984414 1202 70-71 0.17312745613283909 1202 72-73 0.002042278862077751 1202 74-75 0.04073411481921596 1202 76-77 -0.042041591207279794 1202 78-79 -0.31198499919720746 1202 80-81 0.02455026516521741 1202 82-83 -0.09817478949614866 1202 84-85 0.002893089258748205 1202 86-87 -0.008517493408433552 1202 88-89 -0.3772541992435521 1202 90-91 0.4691480123320062 1203 1 0.25865499825333416 1203 2 0.296204723810213 1203 3 0.15247450835995124 1203 4 0.14997382816640226 1203 5 0.08752153019052855 1203 6 0.10838303350494272 1203 7 0.01948908173749686 1203 8 0.25027645174496627 1203 9 0.07890327588739154 1203 10-11 0.08584035028908943 1203 12-13 -0.06960160504802104 1203 14-15 -0.04241763958243183 1203 16-17 0.023894975964907417 1203 18-19 0.1051295282100213 1203 20-21 0.07990682822894968 1203 22-23 0.028201943066434865 1203 24-25 0.09125772727279724 1203 26-27 0.11635354075144733 1203 28-29 0.05776668756636383 1203 30-31 0.07882753161026557 1203 32-33 0.06772301688156546 1203 34-35 0.07449328476028683 1203 36-37 0.03323307084500726 1203 38-39 0.07551763135080591 1203 40-41 -0.06973193080222728 1203 42-43 0.10409081344952398 1203 44-45 0.11286445593710681 1203 46-47 0.07460899412739508 1203 48-49 0.04981738484314491 1203 50-51 0.14533868874553235 1203 52-53 -0.014442619741117824 1203 54-55 0.0988416986972851 1203 56-57 0.0334350842367499 1203 58-59 0.05271620684894884 1203 60-61 -0.0465359930450191 1203 62-63 0.04196486201331595 1203 64-65 -0.22897457663556509 1203 66-67 -0.357021329885157 1203 68-69 -0.1638878798363521 1203 70-71 -0.21080371569333778 1203 72-73 0.1773744824409107 1203 74-75 -0.1568711702702501 1203 76-77 0.18788572349858157 1203 78-79 -0.11019690749127165 1203 80-81 -0.12572842420178176 1203 82-83 -0.0720875360306863 1203 84-85 -0.39287127709385317 1203 86-87 -0.20124476613570863 1203 88-89 0.4350391984156943 1203 90-91 -1.1002299780986178 1204 1 0.2874126669649897 1204 2 0.09993743877759442 1204 3 0.03423636443162792 1204 4 0.11894293421372737 1204 5 -0.1175556438283607 1204 6 0.03084079472804291 1204 7 0.019105646154677913 1204 8 0.03584466676103659 1204 9 -0.07873439574568408 1204 10-11 -0.1218432727511356 1204 12-13 0.028040570431571155 1204 14-15 -0.12234417819378507 1204 16-17 0.03560954164803576 1204 18-19 -0.12364305188541636 1204 20-21 -0.05691881204761273 1204 22-23 -0.08192927711078823 1204 24-25 -0.06271143963732584 1204 26-27 0.01934129515791483 1204 28-29 -0.01947884510478559 1204 30-31 -0.1374909998923073 1204 32-33 -0.08193906894600644 1204 34-35 -0.20514520886607102 1204 36-37 -0.017901067023331052 1204 38-39 -0.03225197972048477 1204 40-41 -0.10995071922512523 1204 42-43 -0.14469313518027604 1204 44-45 -0.0709071218810493 1204 46-47 -0.03607939209229016 1204 48-49 -0.15301629272881456 1204 50-51 0.0015212925546137512 1204 52-53 -0.09254278851673092 1204 54-55 -0.24764299437517678 1204 56-57 -0.021050509415353247 1204 58-59 -0.051766151486880574 1204 60-61 -0.07594229399966679 1204 62-63 0.020556434465362372 1204 64-65 -0.2207630928492108 1204 66-67 0.0901425171203698 1204 68-69 -0.18407043183810856 1204 70-71 -0.05831217822633761 1204 72-73 -0.31574218321705416 1204 74-75 0.07837509051135783 1204 76-77 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0.09576927277342406 2401 54-55 0.15771552264832422 2401 56-57 0.19949820705013366 2401 58-59 0.0486450504171998 2401 60-61 0.10483498741728425 2401 62-63 0.20014018621149887 2401 64-65 0.18939732783447738 2401 66-67 0.24515559471409176 2401 68-69 0.1937841944485328 2401 70-71 0.26773428527568655 2401 72-73 0.3141262830448994 2401 74-75 0.19630283092610767 2401 76-77 0.2521392962600828 2401 78-79 0.2525819683233479 2401 80-81 0.23280075822952995 2401 82-83 0.12371776308551574 2401 84-85 0.1967793482451583 2401 86-87 -0.08272624761718816 2401 88-89 0.23107649445299216 2401 90-91 -0.1022805590965632 2402 1 0.25227585104459394 2402 2 0.3002380192087699 2402 3 0.25117251700210375 2402 4 0.03949641680079452 2402 5 0.10559941421475827 2402 6 0.08054021429686742 2402 7 0.14690415361736342 2402 8 0.03335710457197649 2402 9 0.026520579378697562 2402 10-11 0.09361588976959467 2402 12-13 0.021303422836211894 2402 14-15 0.17054035746127028 2402 16-17 0.07445178874587555 2402 18-19 0.0784454638691443 2402 20-21 0.04869893256266522 2402 22-23 0.08898697496218233 2402 24-25 0.12512541774244568 2402 26-27 0.05795033330053201 2402 28-29 0.07320875854364317 2402 30-31 0.11603304654217794 2402 32-33 0.10928835439996476 2402 34-35 0.09713468364732591 2402 36-37 0.1681246833894221 2402 38-39 0.012666188399784062 2402 40-41 0.074899654845062 2402 42-43 0.13095462026394955 2402 44-45 0.03918600104319836 2402 46-47 0.156326713996485 2402 48-49 0.09844715552092964 2402 50-51 -0.0027138220290865434 2402 52-53 0.1084468842859252 2402 54-55 0.004610519251606604 2402 56-57 -0.14710377119299523 2402 58-59 0.1567812836684439 2402 60-61 0.1681147865859458 2402 62-63 0.04913677017887608 2402 64-65 0.10299370464463209 2402 66-67 0.005545754941962855 2402 68-69 0.11078784723196833 2402 70-71 0.14495575485918977 2402 72-73 0.07405604815095046 2402 74-75 0.24921719707064938 2402 76-77 -0.10685244621184609 2402 78-79 -0.05441955685716948 2402 80-81 0.34427731509700266 2402 82-83 -0.07917438792693332 2402 84-85 0.21997992399263921 2402 86-87 0.015205450314510927 2402 88-89 0.2448732858968441 2402 90-91 0.4691480123320062 2403 1 -0.392625794573469 2403 2 -0.289885210795056 2403 3 -0.2002978236025612 2403 4 -0.16565321963242496 2403 5 0.10539734762463127 2403 6 0.04286857250582443 2403 7 -0.06877896922993898 2403 8 -0.028565589649218737 2403 9 0.15623355297226738 2403 10-11 0.09749112895826784 2403 12-13 0.034574758465765854 2403 14-15 0.07492612095150974 2403 16-17 0.018841379254872948 2403 18-19 -0.04750095786831565 2403 20-21 0.0494957178669182 2403 22-23 0.024305765624248465 2403 24-25 0.10086441506353339 2403 26-27 0.04905710712372269 2403 28-29 0.053199573698634595 2403 30-31 0.09436370096238988 2403 32-33 0.08010198521308354 2403 34-35 0.07580689941313778 2403 36-37 -0.016678251264238497 2403 38-39 9.386506557049756E-4 2403 40-41 0.16471282759805916 2403 42-43 0.061511915279403695 2403 44-45 -8.937519587775E-4 2403 46-47 -0.0023547584385283926 2403 48-49 0.014931199940377837 2403 50-51 0.09294982164379206 2403 52-53 -0.12443778567087804 2403 54-55 0.02742210632595743 2403 56-57 0.11144594851808876 2403 58-59 -0.12638246581632018 2403 60-61 0.03996491987926021 2403 62-63 0.022735441932006495 2403 64-65 0.07481264084167094 2403 66-67 0.09840643514038305 2403 68-69 -0.048923813959206086 2403 70-71 0.011357910390749737 2403 72-73 -0.06860919153572809 2403 74-75 -0.06933756481404885 2403 76-77 0.1006046371247038 2403 78-79 0.06842572813290815 2403 80-81 0.2460028480046219 2403 82-83 0.2705325132188179 2403 84-85 -0.43381215887824 2403 86-87 -0.04795543251849921 2403 88-89 0.20016740354390095 2403 90-91 0.14914801233200592 2404 1 -0.1571512150988923 2404 2 -0.13056863547848252 2404 3 -0.22045582776056705 2404 4 -0.28899625497545856 2404 5 -0.11201032578304648 2404 6 -0.2237063660691163 2404 7 -0.08454914500011057 2404 8 -0.05178842087205027 2404 9 0.034313311052024176 2404 10-11 -0.05004846658133033 2404 12-13 0.08003005992105727 2404 14-15 0.08463695066234322 2404 16-17 0.10453784495133789 2404 18-19 0.0186370122696502 2404 20-21 0.06011299248419277 2404 22-23 0.05957017688866273 2404 24-25 0.07582340202251459 2404 26-27 0.0953462774128937 2404 28-29 0.044219796718863336 2404 30-31 -0.014197251598567107 2404 32-33 0.0644196388164957 2404 34-35 0.10276889269216127 2404 36-37 0.07052351297167547 2404 38-39 0.08192417326435475 2404 40-41 0.10517530900935412 2404 42-43 0.12268742521169429 2404 44-45 0.05425339554013675 2404 46-47 0.10561000149618138 2404 48-49 0.1374421504366481 2404 50-51 0.12213868302662689 2404 52-53 0.2197919852191319 2404 54-55 0.07422930461593324 2404 56-57 0.11946393239166042 2404 58-59 0.0671038361655576 2404 60-61 0.08629012213881282 2404 62-63 0.1749973994322147 2404 64-65 0.2692224245847683 2404 66-67 0.2768877412014277 2404 68-69 0.1926219811753498 2404 70-71 0.26197073971492557 2404 72-73 0.24358640149360866 2404 74-75 0.262022293463545 2404 76-77 0.27628403241192956 2404 78-79 0.1876675717775811 2404 80-81 0.3111534349143028 2404 82-83 0.031236433934260788 2404 84-85 0.28137506026320125 2404 86-87 0.16459844862055206 2404 88-89 0.4920192553957463 2404 90-91 -0.40430111918164613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 29 1.0 30 13.0 31 233.0 32 1708.0 33 3008.0 34 3963.0 35 4991.0 36 7289.0 37 11342.0 38 29408.0 39 180481.0 40 180747.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.4832445997774 15.210892841529667 28.502021612705796 29.803840945987144 2 24.650421951398194 19.291603746162735 29.696114644112175 26.361859658326892 3 25.85950626094395 20.78836615238469 23.15450435867564 30.197623227995717 4 26.354966161291543 22.55071080192068 21.72364739687701 29.370675639910772 5 26.69075390373927 21.989489205641043 21.675205111724452 29.644551778895234 6 26.692171726719348 23.651414042118795 24.469734205452003 25.186680025709858 7 25.47118983704488 24.264858784831183 23.586430488865364 26.67752088925857 8 24.639872963060984 26.047298574615297 23.786107225225905 25.52672123709781 9 23.95931793262505 26.111336912548676 24.562601610646905 25.366743544179364 10-11 23.772874210745208 24.765822904457636 24.750581307421832 26.710721577375324 12-13 24.16383889750085 24.392344701122916 24.514868236984384 26.928948164391848 14-15 24.6060815153692 24.162657378350787 24.205546523498054 27.02571458278196 16-17 24.57158115618738 23.97290540285077 23.963571401565275 27.491942039396573 18-19 24.737939052516165 24.259541948655905 23.884882226171122 27.117636772656812 20-21 24.768776702332794 24.31613671594389 23.73731048432833 27.177776097394986 22-23 24.58445971492306 24.00019849521721 23.95730935006995 27.458032439789786 24-25 24.751053915081854 24.293806004007713 23.94431263941926 27.01082744149117 26-27 24.887046769254038 24.338940035540098 23.75491511966426 27.01909807554161 28-29 24.93997882717683 24.094129267647173 23.82781485122311 27.13807705395289 30-31 25.039197221989202 24.20751901375418 23.8899240395056 26.863359724751025 32-33 25.03257028117299 24.22045643068356 23.962550515630713 26.784422772512734 34-35 24.960987347551278 23.969174079382483 24.057615232335785 27.012223340730458 36-37 25.056827301958794 24.322214685521075 24.032123606501354 26.588834406018773 38-39 25.11348976298048 24.28667433161142 24.043096421766283 26.556739483641824 40-41 25.19948218015721 23.9935442111303 24.09628848670543 26.71068512200706 42-43 25.122281334173106 24.334618311006288 24.127800931199346 26.415299423621253 44-45 25.304572809732456 24.365351778244822 24.008867179924604 26.32120823209812 46-47 25.244428046755772 24.150497241275218 24.085260527595633 26.519814184373374 48-49 25.423073688279196 24.398964259064076 24.024071850501137 26.153890202155587 50-51 25.47736485773539 24.359085040297593 23.9314566798466 26.232093422120418 52-53 25.473862626346143 24.181152979598586 23.91595526984849 26.42902912420678 54-55 25.434553241713537 24.5180572072354 24.049973622797058 25.997415928254007 56-57 25.60915498942553 24.474493797363543 23.804575066591234 26.111776146619693 58-59 25.769959332900612 24.258785815527677 23.760978129843295 26.21027672172842 60-61 25.791430918713043 24.396248160222488 23.801423051577636 26.010897869486836 62-63 25.838986362267875 24.28592475866531 23.67065741683802 26.2044314622288 64-65 25.75817852449117 24.287997726707633 23.680957624409476 26.27286612439172 66-67 25.9989241442596 24.618901836074393 23.36901060825403 26.013163411411984 68-69 26.118374707031684 24.549083546367637 23.342209147257282 25.990332599343397 70-71 26.339144692308846 24.29037741241595 23.40538616204616 25.96509173322904 72-73 26.190462538774245 24.4399594052991 23.294363184963906 26.07521487096275 74-75 26.412628925811692 24.334560408582895 23.12207740523331 26.130733260372104 76-77 26.727368863039047 24.233706047929335 22.857409452125097 26.18151563690652 78-79 26.878842877250165 24.36291577153863 22.835950811171198 25.922290540040006 80-81 27.112930140260676 24.171289406034365 22.493573264781492 26.222207188923463 82-83 27.508109030899675 23.886075229044557 22.18602401411256 26.41979172594321 84-85 28.16599631620494 23.538698643010186 21.548321617862648 26.746983422922227 86-87 28.369415260532737 23.049984081502707 20.643637907248223 27.936962750716333 88-89 28.300907911802852 22.06658019887592 20.722006052745353 28.91050583657588 90-91 26.82439537329127 23.83806519453207 21.577287066246058 27.760252365930597 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 2.5 11 3.5 12 7.0 13 11.0 14 19.5 15 29.0 16 34.0 17 43.0 18 59.0 19 92.0 20 146.5 21 213.5 22 298.5 23 424.0 24 564.0 25 703.5 26 915.5 27 1218.0 28 1578.0 29 2008.0 30 2490.0 31 3067.0 32 3820.0 33 4664.5 34 5728.5 35 6938.5 36 8388.0 37 10001.5 38 11643.5 39 13618.5 40 15682.0 41 17707.0 42 19816.0 43 21850.0 44 23742.0 45 25457.5 46 27088.5 47 28639.5 48 29589.0 49 30074.5 50 30501.5 51 30710.0 52 30371.5 53 29860.0 54 29385.0 55 28605.0 56 27421.5 57 26062.0 58 24943.0 59 23835.5 60 22541.5 61 21287.5 62 20145.0 63 18794.5 64 17328.0 65 15943.5 66 14366.0 67 12808.5 68 11272.0 69 9642.0 70 8133.5 71 6845.0 72 5702.5 73 4578.5 74 3534.0 75 2749.5 76 2144.5 77 1586.5 78 1190.5 79 862.5 80 582.5 81 395.5 82 262.0 83 179.0 84 129.0 85 90.5 86 52.5 87 28.0 88 22.0 89 17.5 90 9.0 91 5.0 92 6.0 93 6.5 94 3.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001654126810087338 2 0.0073254187303867825 3 0.0011815191500623842 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 5958.0 32-33 12933.0 34-35 14242.0 36-37 15439.0 38-39 16482.0 40-41 17689.0 42-43 18573.0 44-45 19062.0 46-47 18715.0 48-49 18695.0 50-51 18893.0 52-53 18297.0 54-55 17924.0 56-57 17322.0 58-59 16610.0 60-61 16277.0 62-63 15615.0 64-65 14568.0 66-67 13674.0 68-69 13303.0 70-71 12622.0 72-73 12046.0 74-75 11239.0 76-77 10530.0 78-79 9835.0 80-81 9276.0 82-83 8697.0 84-85 7979.0 86-87 7419.0 88-89 6865.0 90-91 6405.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.80631632643244 #Duplication Level Percentage of deduplicated Percentage of total 1 99.81406795364336 99.62074440009353 2 0.17888077390823892 0.35706862210805473 3 0.005975056049227456 0.017890450023520778 4 0.0010762163991847814 0.004296527774909215 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0025993421301372453 0.0 0.0 0.0 0.0 2 0.0025993421301372453 0.0 0.0 0.0 0.0 3 0.0028356459601497222 0.0 0.0 0.0 0.0 4 0.0028356459601497222 0.0 0.0 0.0 0.0 5 0.003071949790162199 0.0 0.0 0.0 0.0 6 0.003071949790162199 0.0 0.0 0.0 0.0 7 0.0033082536201746757 0.0 0.0 0.0 0.0 8 0.009924760860524027 0.0 0.0 0.0 0.0 9 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 10-11 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 12-13 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 14-15 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 16-17 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 18-19 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 20-21 0.010161064690536504 2.3630383001247684E-4 0.0 0.0 0.0 22-23 0.010279216605542742 2.3630383001247684E-4 0.0 0.0 0.0 24-25 0.010633672350561459 2.3630383001247684E-4 0.0 0.0 0.0 26-27 0.010633672350561459 2.3630383001247684E-4 0.0 0.0 0.0 28-29 0.010633672350561459 2.3630383001247684E-4 0.0 0.0 0.0 30-31 0.010633672350561459 2.3630383001247684E-4 0.0 0.0 1.1815191500623842E-4 32-33 0.010869976180573934 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 34-35 0.011697039585617602 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 36-37 0.012169647245642557 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 38-39 0.01276040682067375 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 40-41 0.01346931831071118 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 42-43 0.013705622140723657 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 44-45 0.014060077885742372 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 46-47 0.014414533630761088 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 48-49 0.014768989375779803 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 50-51 0.015005293205792278 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 52-53 0.015359748950810995 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 54-55 0.015596052780823471 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 56-57 0.015596052780823471 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 58-59 0.015596052780823471 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 60-61 0.015596052780823471 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 62-63 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 64-65 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 66-67 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 68-69 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 70-71 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 72-73 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 74-75 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 76-77 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 78-79 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 80 0.0163049642708609 2.3630383001247684E-4 0.0 0.0 2.3630383001247684E-4 >>END_MODULE