##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-12A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 514444 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 52 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.24672073150819 40.0 40.0 40.0 40.0 40.0 2 39.16142087379773 40.0 40.0 40.0 40.0 40.0 3 39.14308651670542 40.0 40.0 40.0 40.0 40.0 4 39.055640652821296 40.0 40.0 40.0 40.0 40.0 5 39.19615740488761 40.0 40.0 40.0 40.0 40.0 6 39.26321232242965 40.0 40.0 40.0 40.0 40.0 7 39.278102184105556 40.0 40.0 40.0 40.0 40.0 8 39.3072598766824 40.0 40.0 40.0 40.0 40.0 9 39.33333074153844 40.0 40.0 40.0 40.0 40.0 10-11 39.316827487539946 40.0 40.0 40.0 40.0 40.0 12-13 39.316563124460586 40.0 40.0 40.0 40.0 40.0 14-15 39.3282067630296 40.0 40.0 40.0 40.0 40.0 16-17 39.46821811509125 40.0 40.0 40.0 40.0 40.0 18-19 39.488251393737706 40.0 40.0 40.0 40.0 40.0 20-21 39.49622505073438 40.0 40.0 40.0 40.0 40.0 22-23 39.49772569997901 40.0 40.0 40.0 40.0 40.0 24-25 39.4897675937517 40.0 40.0 40.0 40.0 40.0 26-27 39.48110192751786 40.0 40.0 40.0 40.0 40.0 28-29 39.48063151674429 40.0 40.0 40.0 40.0 40.0 30-31 39.474741976330655 40.0 40.0 40.0 40.0 40.0 32-33 39.4733321822347 40.0 40.0 40.0 40.0 40.0 34-35 39.46759918801666 40.0 40.0 40.0 40.0 40.0 36-37 39.46433334985819 40.0 40.0 40.0 40.0 40.0 38-39 39.44686711699687 40.0 40.0 40.0 40.0 40.0 40-41 39.436878893877214 40.0 40.0 40.0 40.0 40.0 42-43 39.435119258505175 40.0 40.0 40.0 40.0 40.0 44-45 39.414552360519025 40.0 40.0 40.0 40.0 40.0 46-47 39.415513200221476 40.0 40.0 40.0 40.0 40.0 48-49 39.406536626734436 40.0 40.0 40.0 40.0 40.0 50-51 39.40809633825872 40.0 40.0 40.0 40.0 40.0 52-53 39.39349947855214 40.0 40.0 40.0 40.0 40.0 54-55 39.39150051322913 40.0 40.0 40.0 40.0 40.0 56-57 39.37590494114793 40.0 40.0 40.0 40.0 40.0 58-59 39.35811884789973 40.0 40.0 40.0 40.0 40.0 60-61 39.328209716687276 40.0 40.0 40.0 40.0 40.0 62-63 39.38048394245645 40.0 40.0 40.0 40.0 40.0 64-65 39.3737351549037 40.0 40.0 40.0 40.0 40.0 66-67 39.36834836378533 40.0 40.0 40.0 40.0 40.0 68-69 39.364876867361126 40.0 40.0 40.0 40.0 40.0 70-71 39.35212494273027 40.0 40.0 40.0 40.0 40.0 72-73 39.359266724130094 40.0 40.0 40.0 40.0 40.0 74-75 39.35998827187335 40.0 40.0 40.0 40.0 40.0 76-77 39.3516236059684 40.0 40.0 40.0 40.0 40.0 78-79 39.35520242680971 40.0 40.0 40.0 40.0 40.0 80-81 39.35187793955269 40.0 40.0 40.0 40.0 40.0 82-83 39.33285269388446 40.0 40.0 40.0 40.0 40.0 84-85 39.334944913541264 40.0 40.0 40.0 40.0 40.0 86-87 39.333261472295774 40.0 40.0 40.0 40.0 40.0 88-89 39.34173312136113 40.0 40.0 40.0 40.0 40.0 90-91 39.40671788513256 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2948318204652338 1101 2 0.2331263352554913 1101 3 0.23389302162706826 1101 4 0.09721828335269578 1101 5 0.06284596078191385 1101 6 0.1158125988803036 1101 7 -0.044108900163543296 1101 8 0.07843905007404572 1101 9 0.11478434971828477 1101 10-11 0.057649380772282655 1101 12-13 -0.10995266758601474 1101 14-15 0.020168993641462407 1101 16-17 0.04417036431278376 1101 18-19 0.054023391017146594 1101 20-21 -0.02352763862602103 1101 22-23 -0.009417143797051608 1101 24-25 -0.02910799076376236 1101 26-27 -0.027062073125492248 1101 28-29 0.09095064490025351 1101 30-31 0.04910855370166445 1101 32-33 0.033762769590445885 1101 34-35 0.09849588008486165 1101 36-37 0.05045095452274495 1101 38-39 0.12889127148753232 1101 40-41 0.11133108862244256 1101 42-43 0.05555357562203511 1101 44-45 0.059707751707058776 1101 46-47 0.0861670298781334 1101 48-49 0.10572101623009189 1101 50-51 0.02811938007376824 1101 52-53 0.04479671726497969 1101 54-55 0.01611864227781723 1101 56-57 0.14022017768830608 1101 58-59 0.08218007906751978 1101 60-61 -0.039212841699061585 1101 62-63 -0.015310428223983763 1101 64-65 0.24970391602239062 1101 66-67 -0.027047827222524745 1101 68-69 -0.059668994959167776 1101 70-71 -0.005212835475184363 1101 72-73 0.012964477028603483 1101 74-75 -0.12699110834675054 1101 76-77 -0.2708920112306501 1101 78-79 -0.3654807142603289 1101 80-81 -0.5780316223482131 1101 82-83 -0.13101141143121708 1101 84-85 -0.3248652201807616 1101 86-87 -0.0342297993406433 1101 88-89 -0.09673402376222384 1101 90-91 -0.6581483775055972 1102 1 0.3511962303238505 1102 2 0.06340087233709824 1102 3 0.20055233915042692 1102 4 -0.021805543397981353 1102 5 0.07572662829114307 1102 6 0.014160904799261687 1102 7 0.04075693656747603 1102 8 0.01101241988397561 1102 9 -0.013547839839922915 1102 10-11 -0.10659962476441365 1102 12-13 -0.06301462086409515 1102 14-15 0.07343434222376288 1102 16-17 0.025299006408474156 1102 18-19 -0.023290424647711916 1102 20-21 -0.03532891365063051 1102 22-23 0.01705716163127846 1102 24-25 -0.018841962126948886 1102 26-27 3.5126650119110536E-4 1102 28-29 -0.0011231487792784378 1102 30-31 -0.026055171755039908 1102 32-33 0.15539757217987216 1102 34-35 -0.11216664090211026 1102 36-37 -0.0365554260187011 1102 38-39 0.04321878841096094 1102 40-41 0.0042389785878285124 1102 42-43 0.00532468314646195 1102 44-45 0.023242980885100906 1102 46-47 0.010277188843829776 1102 48-49 -0.12614263006026505 1102 50-51 -0.05766343853876066 1102 52-53 -0.27528569447861884 1102 54-55 -0.1867741705292616 1102 56-57 -0.08959520582396863 1102 58-59 0.10864591563876758 1102 60-61 -0.22470971282167795 1102 62-63 -0.04603906644446454 1102 64-65 0.08465686242572446 1102 66-67 0.1974196817128302 1102 68-69 0.18642818741885492 1102 70-71 0.12337220376955571 1102 72-73 0.06592730537013125 1102 74-75 -0.407865015275263 1102 76-77 0.12106951065329241 1102 78-79 0.03497448923887703 1102 80-81 -0.17601385064622832 1102 82-83 -0.188371901986784 1102 84-85 -0.40428670002923894 1102 86-87 -0.2687838424260889 1102 88-89 0.3342424072142052 1102 90-91 0.602457683100468 1103 1 0.3387572968785122 1103 2 0.14863413907556122 1103 3 0.21431133267196856 1103 4 0.21267288286509256 1103 5 0.07635359560006094 1103 6 -0.01984962261862222 1103 7 0.08898198844228489 1103 8 0.10612531992003227 1103 9 0.06911877947764822 1103 10-11 0.055372236403961494 1103 12-13 0.023814228693751716 1103 14-15 -0.05069538857827638 1103 16-17 0.03856450808333989 1103 18-19 0.01649995764928036 1103 20-21 0.04053535527425822 1103 22-23 -0.03064378475873042 1103 24-25 0.05867911689358607 1103 26-27 0.032445380172077876 1103 28-29 0.0011079731677057225 1103 30-31 0.07324077579522026 1103 32-33 0.07581407225481485 1103 34-35 0.09675559614763785 1103 36-37 0.1389265562910964 1103 38-39 0.0017908801500610139 1103 40-41 -0.023570486066311958 1103 42-43 -0.04333918956601934 1103 44-45 0.09250932387558919 1103 46-47 0.06738449068046748 1103 48-49 0.05354182403976182 1103 50-51 0.0609866210093557 1103 52-53 0.10411984656227702 1103 54-55 0.17151175843281408 1103 56-57 0.17157099099986794 1103 58-59 0.06957622671754393 1103 60-61 -0.08501096635433214 1103 62-63 0.12846964220767632 1103 64-65 0.1320308130479333 1103 66-67 0.04030327467297212 1103 68-69 0.02244930163483616 1103 70-71 -0.08591643711331898 1103 72-73 -0.11681153895896301 1103 74-75 0.21696280757431907 1103 76-77 -0.023732329070675462 1103 78-79 0.28385164064246027 1103 80-81 0.12396783673113987 1103 82-83 0.12073492580374534 1103 84-85 -0.06457307028355075 1103 86-87 0.12560584226712734 1103 88-89 0.4200123877210302 1103 90-91 -0.397542316899532 1104 1 0.4531116315956112 1104 2 0.19193593236818884 1104 3 0.1022841853577603 1104 4 0.21906783998461776 1104 5 0.2909404687485164 1104 6 0.2356673802140321 1104 7 0.003926654633332305 1104 8 0.05432616725185113 1104 9 0.1610787436803136 1104 10-11 -0.006566289065581543 1104 12-13 0.14809517166143848 1104 14-15 0.13498074891570866 1104 16-17 0.08212474419188709 1104 18-19 0.0110113534748848 1104 20-21 0.1383661786069581 1104 22-23 0.07622772889276064 1104 24-25 -0.07794096795979755 1104 26-27 0.05111336589882143 1104 28-29 0.141480888120185 1104 30-31 -0.0922900447126338 1104 32-33 0.09065328291151076 1104 34-35 0.09573371053657098 1104 36-37 0.12396506156173359 1104 38-39 0.06350533629348121 1104 40-41 0.06919283404760534 1104 42-43 -0.030327944085044578 1104 44-45 -0.024636433026699933 1104 46-47 -8.634085481702414E-4 1104 48-49 -0.12410994534927511 1104 50-51 -0.0534619992105263 1104 52-53 -0.044321571389460246 1104 54-55 0.046893930956827035 1104 56-57 -0.33792940857057374 1104 58-59 -0.11071226273325863 1104 60-61 -0.13252492728233278 1104 62-63 -0.10984357554323054 1104 64-65 -0.18681819548687173 1104 66-67 -0.04753475103376559 1104 68-69 -0.19947726409363042 1104 70-71 -0.199908513792856 1104 72-73 -0.05608214816194845 1104 74-75 0.19577099712297752 1104 76-77 0.24421801202127824 1104 78-79 0.23734257647704737 1104 80-81 0.11640223326985222 1104 82-83 -0.05492442753327964 1104 84-85 -0.12093656462430857 1104 86-87 -0.5024590561013014 1104 88-89 0.2749831479549485 1104 90-91 0.602457683100468 1201 1 0.32957118259660234 1201 2 0.1560417254576265 1201 3 0.32390118103744214 1201 4 0.3169377550532886 1201 5 0.06373064410414031 1201 6 0.16155550871134494 1201 7 0.12556379689517883 1201 8 0.07598345609586943 1201 9 0.12885798764968115 1201 10-11 0.042724534022745786 1201 12-13 0.035510789911825213 1201 14-15 0.10050541240751443 1201 16-17 0.011469360376537452 1201 18-19 0.03127387893793099 1201 20-21 -0.11550601170534947 1201 22-23 -0.044745896303901134 1201 24-25 0.040106524020309564 1201 26-27 0.17418544991064522 1201 28-29 0.054239873581920506 1201 30-31 0.09546117771424178 1201 32-33 -0.022307451184609306 1201 34-35 -0.007314709117608231 1201 36-37 -0.009717131714246818 1201 38-39 0.003455739601527341 1201 40-41 0.02990755542571577 1201 42-43 -0.032875709327562674 1201 44-45 -0.11932668199365537 1201 46-47 0.035563396850179174 1201 48-49 -0.13219205531722622 1201 50-51 0.0036283586202259244 1201 52-53 -0.024352191319962913 1201 54-55 0.05913837393131871 1201 56-57 0.05822350093917805 1201 58-59 0.07045448659589226 1201 60-61 -0.05583570281937256 1201 62-63 -0.16443775491930523 1201 64-65 0.0538815956335057 1201 66-67 -0.15139744933198074 1201 68-69 -0.247275181053638 1201 70-71 -0.2878368977368737 1201 72-73 -0.042275588334469205 1201 74-75 -0.07023371231396425 1201 76-77 -0.34195759026409434 1201 78-79 -0.3530161422465312 1201 80-81 -0.5047358911956081 1201 82-83 -0.4422106819432159 1201 84-85 -0.2625180105535776 1201 86-87 -1.099616944818635 1201 88-89 0.1722053701771742 1201 90-91 0.602457683100468 1202 1 -0.034673898207785214 1202 2 0.0017860315566906593 1202 3 0.034607219680836465 1202 4 0.14772340037521303 1202 5 0.044598888964003436 1202 6 0.24182275809318554 1202 7 -0.034580671979362876 1202 8 0.017941146870931846 1202 9 0.04090286588652248 1202 10-11 0.05830431176207895 1202 12-13 0.030028953637582845 1202 14-15 -0.10471569001309433 1202 16-17 -0.0073512726554980645 1202 18-19 0.08013921322792328 1202 20-21 -0.005247042004249636 1202 22-23 -0.03653272355165882 1202 24-25 -0.014698311673960518 1202 26-27 -0.030683949257330312 1202 28-29 -0.0922073317581038 1202 30-31 -0.013386469960259717 1202 32-33 0.01524063130505482 1202 34-35 -0.03298504907449029 1202 36-37 0.011380204273869765 1202 38-39 -0.05320216639732678 1202 40-41 0.0037186799454858033 1202 42-43 -0.06555862226461784 1202 44-45 -0.12445452609230756 1202 46-47 -0.01940158490760524 1202 48-49 0.14943688531941035 1202 50-51 0.015705726200330616 1202 52-53 -0.04504430673476634 1202 54-55 -0.0681337647140623 1202 56-57 -0.07731212291092504 1202 58-59 -0.213633703108556 1202 60-61 -0.2265052515847472 1202 62-63 -0.4146833396773246 1202 64-65 -0.29848107469451435 1202 66-67 -0.17820539594107032 1202 68-69 -0.2646398130339449 1202 70-71 -0.21979435880434295 1202 72-73 0.039617912102237085 1202 74-75 -0.34966126620042814 1202 76-77 -0.43212493236901395 1202 78-79 -0.4346557924512666 1202 80-81 -0.3860123451610846 1202 82-83 -0.18372138101143776 1202 84-85 -0.14599386026983296 1202 86-87 -0.7613328620339317 1202 88-89 0.12260219557399665 1202 90-91 -1.4292883486455636 1203 1 0.21786887315067816 1203 2 0.17989757739670864 1203 3 -0.05910793942788928 1203 4 0.09264568502308634 1203 5 0.13571951510829905 1203 6 0.020773487216430908 1203 7 0.0683993937704983 1203 8 0.16841283677935337 1203 9 0.00279734627574868 1203 10-11 0.08438991676126051 1203 12-13 0.13848985805451264 1203 14-15 -0.0477784845562752 1203 16-17 0.12584634894780322 1203 18-19 0.043880839490611834 1203 20-21 0.09938266191936407 1203 22-23 0.038304739394604326 1203 24-25 0.06612273681137282 1203 26-27 0.06368769851237488 1203 28-29 0.015975266354686823 1203 30-31 0.013779816373165943 1203 32-33 0.07559323008057817 1203 34-35 -0.043265069157193636 1203 36-37 0.009342936601164809 1203 38-39 0.008041216748104318 1203 40-41 0.0070355645034325676 1203 42-43 0.010130183714821328 1203 44-45 0.0635473688853665 1203 46-47 0.03834923200864182 1203 48-49 0.0030344958028507563 1203 50-51 0.06640006464120063 1203 52-53 0.12590866075721152 1203 54-55 0.06257220672330277 1203 56-57 0.0422205423490567 1203 58-59 0.09226436171783803 1203 60-61 0.10655883473927474 1203 62-63 0.005935807546052274 1203 64-65 -0.004764531426573626 1203 66-67 -0.04854525527861142 1203 68-69 -0.12126577059216004 1203 70-71 0.03464656098937979 1203 72-73 -0.25471159733030646 1203 74-75 -0.02463983818908133 1203 76-77 -0.09023225391505463 1203 78-79 -0.1925424148421513 1203 80-81 0.10212602193546871 1203 82-83 0.5178569196389446 1203 84-85 0.035669175314765766 1203 86-87 0.41542010156945963 1203 88-89 0.4200123877210302 1203 90-91 0.602457683100468 1204 1 0.16409740190348288 1204 2 -0.03518830759208669 1204 3 -0.008323772138865593 1204 4 -0.322589565163625 1204 5 -0.07936636988050338 1204 6 -0.10469327902703895 1204 7 -0.1974706585073207 1204 8 -0.13031755903841713 1204 9 -0.2839838337093141 1204 10-11 -0.08289910489668983 1204 12-13 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0.16440225113204576 2401 62-63 0.15378236851554306 2401 64-65 0.017651226597799052 2401 66-67 -0.03255838478014539 2401 68-69 0.002901908116875518 2401 70-71 0.10713565278518189 2401 72-73 0.2575765499427902 2401 74-75 0.14785017064963313 2401 76-77 0.35992621303568484 2401 78-79 0.2439136522728802 2401 80-81 0.3433663187240015 2401 82-83 0.19055463637049286 2401 84-85 0.31560639619049624 2401 86-87 -0.25409304302940683 2401 88-89 -0.09673402376223095 2401 90-91 0.050733545169435956 2402 1 0.3797704971311191 2402 2 0.31124523221667033 2402 3 0.35687560111304606 2402 4 0.3839927540971644 2402 5 0.25081565064989064 2402 6 0.06526967254642102 2402 7 0.10759018584536051 2402 8 0.2051010296068938 2402 9 0.07279544260165238 2402 10-11 0.1694804877170597 2402 12-13 0.10230028140234992 2402 14-15 0.08397260273450513 2402 16-17 -0.054575827390991094 2402 18-19 0.003550771690527199 2402 20-21 0.051267299269454725 2402 22-23 0.04625038217748312 2402 24-25 -0.006883164578304957 2402 26-27 0.11182941972662519 2402 28-29 0.03348247835076279 2402 30-31 0.15602307986649322 2402 32-33 0.05239318554270511 2402 34-35 0.019364117908708067 2402 36-37 0.04803552208731787 2402 38-39 0.13366398978657656 2402 40-41 -0.024503092169531726 2402 42-43 0.032060051207466245 2402 44-45 0.08799666109671023 2402 46-47 0.14419043838648093 2402 48-49 0.13488448188423519 2402 50-51 0.04698284625838056 2402 52-53 0.17013259604512854 2402 54-55 0.05651008693382664 2402 56-57 0.07957139563072246 2402 58-59 0.23357969844599324 2402 60-61 0.2663847574930145 2402 62-63 0.18147675015728737 2402 64-65 0.09298037432833439 2402 66-67 0.04729015309114004 2402 68-69 0.0871631139016742 2402 70-71 0.1465981090833992 2402 72-73 0.15913919384586706 2402 74-75 0.2841967631540996 2402 76-77 0.1134563251955214 2402 78-79 0.09862726070799965 2402 80-81 0.44890228343489724 2402 82-83 0.5136729159403828 2402 84-85 0.2688080576784557 2402 86-87 -0.04134794499018568 2402 88-89 0.31681321331442547 2402 90-91 0.602457683100468 2403 1 -0.31221749423767875 2403 2 -0.23751168681889112 2403 3 -0.14809672274472518 2403 4 -0.02019150333786257 2403 5 0.16433474249716085 2403 6 0.08150008815763954 2403 7 -0.04470365034940471 2403 8 -0.033701859969987424 2403 9 0.002704439693317795 2403 10-11 0.10691833366883685 2403 12-13 0.034869002670760096 2403 14-15 0.031129934566763495 2403 16-17 0.05621786807762419 2403 18-19 0.01665706684016044 2403 20-21 0.0018864943158973801 2403 22-23 0.0826890724861471 2403 24-25 0.07253329099913941 2403 26-27 0.013335189498654643 2403 28-29 -0.0028483219119266323 2403 30-31 -0.03676422313357364 2403 32-33 -0.055475478160509795 2403 34-35 -0.012537625069462877 2403 36-37 0.03463527334405114 2403 38-39 0.02780250641213655 2403 40-41 0.12285648455988962 2403 42-43 0.042803321490666235 2403 44-45 0.029056445803433917 2403 46-47 0.029184705323373805 2403 48-49 0.04878915418618135 2403 50-51 0.16553578500964505 2403 52-53 0.022520227145101046 2403 54-55 -0.047234886599056836 2403 56-57 -0.029900456385377083 2403 58-59 -0.10022326248989089 2403 60-61 0.09513822902364666 2403 62-63 0.15676914781811035 2403 64-65 0.15215375993450664 2403 66-67 0.10117374259858991 2403 68-69 -0.005313673963605936 2403 70-71 -0.04002281716017109 2403 72-73 -0.051967951638140164 2403 74-75 0.00418260587201047 2403 76-77 0.10721818355853685 2403 78-79 -0.00638812808644218 2403 80-81 0.25620131609657193 2403 82-83 0.08982161001360112 2403 84-85 0.120188323559411 2403 86-87 -0.23148615251247406 2403 88-89 0.09207716504896268 2403 90-91 -0.01292693228414521 2404 1 -0.5463114588300755 2404 2 -0.25585737041378565 2404 3 -0.399784943899661 2404 4 -0.32729520479499996 2404 5 -0.31288479641634837 2404 6 -0.13993187827258424 2404 7 -0.2367973705016837 2404 8 -0.10291462698686615 2404 9 -0.05224026704644302 2404 10-11 0.04240515427242286 2404 12-13 0.026151543761592677 2404 14-15 0.07628436187525267 2404 16-17 0.05315693458797455 2404 18-19 0.07525389002395144 2404 20-21 -0.019813450911414066 2404 22-23 0.03621828987036224 2404 24-25 0.022658453393383127 2404 26-27 0.10039027453212412 2404 28-29 0.028573097379833712 2404 30-31 -0.02555890701365371 2404 32-33 -0.034598220111512035 2404 34-35 0.0635617959350796 2404 36-37 0.016890320850770024 2404 38-39 0.030448266684004466 2404 40-41 0.01630584932532031 2404 42-43 0.09501175822728669 2404 44-45 0.02307176370083397 2404 46-47 0.07682595365667311 2404 48-49 0.06791997813630957 2404 50-51 0.10907805029828666 2404 52-53 0.030846231023474502 2404 54-55 -0.01739451985275764 2404 56-57 0.12375299781187721 2404 58-59 0.041051470939905244 2404 60-61 0.08368221995098679 2404 62-63 0.2371889935164333 2404 64-65 0.23008805582852432 2404 66-67 0.1450412989161265 2404 68-69 -0.01778139989617955 2404 70-71 0.09122727587485713 2404 72-73 0.31819073884592086 2404 74-75 0.3554162324604704 2404 76-77 0.020218208645822244 2404 78-79 0.021536881674421693 2404 80-81 0.24712442254764966 2404 82-83 -0.44847815138309954 2404 84-85 -0.4559731127756095 2404 86-87 -0.13813525157293327 2404 88-89 0.25844568025468817 2404 90-91 0.03102911167189859 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 12.0 31 300.0 32 2052.0 33 3755.0 34 4986.0 35 6255.0 36 8858.0 37 14069.0 38 36799.0 39 219097.0 40 218261.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 26.64471382251631 14.370883841721808 28.47759488060711 30.50680745515477 2 25.150125485271275 18.721897034037514 29.2568278178782 26.87114966281301 3 26.09337125151378 20.15935743846233 22.743910380393476 31.003360929630414 4 26.71447232351821 21.635785430484173 21.23438119600967 30.41536104998795 5 27.061060095948246 21.319910427568402 21.159737503012963 30.459291973470386 6 27.30520717512499 23.32673721532373 24.051208683549618 25.316846926001663 7 25.747020083818644 23.660689987637138 23.405657369898375 27.18663255864584 8 24.606176765595478 25.175723693929758 23.89958868215005 26.318510858324718 9 24.332483224607536 25.22451423284167 24.533476918770557 25.90952562378024 10-11 23.98647860602903 23.987742106040695 24.594124919330383 27.431654368599883 12-13 24.40829322530732 23.64037679514194 24.37971868658202 27.57161129296872 14-15 24.931187845518657 23.206704714215736 24.18970772328961 27.672399716976 16-17 25.006414692366906 23.085797482330438 23.848854297066346 28.058933528236306 18-19 25.059481692856757 23.459015947313993 23.716089603533135 27.765412756296115 20-21 25.10953573178033 23.528508447955463 23.624631641150444 27.73732417911376 22-23 24.963261307353182 23.057222943605137 23.829124258422684 28.150391490618997 24-25 25.08066961612926 23.489923101445445 23.810754912099277 27.618652370326025 26-27 25.255032617738767 23.528119678721104 23.73844383450871 27.47840386903142 28-29 25.39460077287324 23.125840713469298 23.683432987847077 27.79612552581039 30-31 25.304286890320647 23.518852034394648 23.738508146228767 27.438352929055938 32-33 25.420090214298806 23.431211597159685 23.814741909004695 27.333956279536814 34-35 25.311670861524256 23.216445356514697 23.96429017742337 27.50759360453768 36-37 25.358538222562625 23.531854286968272 23.934389549143294 27.17521794132581 38-39 25.466961824879046 23.55601122261449 23.949005304069605 27.028021648436855 40-41 25.363571152940175 23.355673556546176 24.055827673310336 27.224927617203313 42-43 25.461476675316096 23.683380834298784 24.008047222382086 26.847095268003034 44-45 25.496648255551246 23.681629463455085 23.9867684505425 26.83495383045117 46-47 25.409592403536635 23.513449239419394 24.018945860476705 27.05801249656727 48-49 25.6000266443016 23.809105690072272 23.949593825788657 26.64127383983747 50-51 25.744083198740437 23.766096253031392 23.854089916186442 26.63573063204173 52-53 25.66817872891938 23.53934962005265 23.99823680545677 26.794234845571204 54-55 25.69723390888842 23.92830626891812 23.86986236174304 26.50459746045042 56-57 25.7457212207997 23.793757772779287 23.851077170118593 26.60944383630242 58-59 25.831700775267368 23.74021877407882 23.84574190443265 26.582338546221163 60-61 25.945962005540963 23.99820748153632 23.56938952862211 26.486440984300607 62-63 26.09450305931412 23.98245738520067 23.560812396635573 26.36222715884963 64-65 26.034728721615945 23.70797100790831 23.74128828837015 26.516011982105592 66-67 26.072331961081634 24.217597017294423 23.353044676417646 26.357026345206293 68-69 26.367232600836598 23.849109458103836 23.231313762231938 26.552344178827624 70-71 26.387225034672575 23.716900035638684 23.44767768889576 26.448197240792982 72-73 26.380389190675928 24.130902446930776 23.059694894942094 26.429013467451203 74-75 26.556471015479595 24.09948250034046 23.114984792773164 26.22906169140678 76-77 26.65674359264009 23.966881740436303 22.93293286641726 26.44344180050634 78-79 26.774576539496596 24.088174766503087 22.655532689567835 26.48171600443248 80-81 27.20416401104738 24.078258686289278 22.383490739131272 26.334086563532072 82-83 27.5571468585973 23.558997966217284 22.095161545674863 26.78869362951055 84-85 28.13400518376612 23.20459777516622 21.401548690374618 27.259848350693044 86-87 29.154013015184383 22.09612969517068 20.774060965863683 27.975796323781253 88-89 28.21787730382444 21.26060787319152 21.144715690306178 29.376799132677856 90-91 26.138098231121486 23.471311843404866 20.867379006913893 29.523210918559755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 2.0 6 2.0 7 2.0 8 1.0 9 0.0 10 0.5 11 3.0 12 6.5 13 11.5 14 19.0 15 27.0 16 33.5 17 52.0 18 85.0 19 120.0 20 170.5 21 220.5 22 270.0 23 370.5 24 541.5 25 723.5 26 930.0 27 1212.5 28 1586.0 29 2058.0 30 2579.5 31 3168.0 32 3902.5 33 4783.0 34 5948.0 35 7323.5 36 8857.0 37 10457.0 38 12361.0 39 14742.0 40 17116.0 41 19459.5 42 21898.0 43 24347.5 44 26836.0 45 29177.0 46 31229.0 47 33034.5 48 34472.5 49 35746.0 50 36747.0 51 37074.0 52 37069.5 53 36895.5 54 36304.5 55 35602.5 56 34855.0 57 33784.5 58 32510.0 59 31182.5 60 29783.0 61 28391.0 62 27064.0 63 25529.0 64 23647.0 65 21779.5 66 19752.5 67 17763.0 68 15890.0 69 13820.0 70 11819.5 71 10019.5 72 8389.5 73 6774.0 74 5285.5 75 4144.0 76 3152.0 77 2281.5 78 1694.5 79 1231.5 80 854.0 81 594.0 82 403.5 83 272.5 84 191.0 85 134.0 86 89.5 87 57.5 88 40.5 89 27.5 90 16.5 91 11.0 92 6.0 93 1.5 94 1.0 95 2.0 96 2.0 97 2.0 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015550769374314795 2 0.007969769304336332 3 5.831538515368048E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.377146176395099E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 8292.0 32-33 17411.0 34-35 18987.0 36-37 20176.0 38-39 21592.0 40-41 22520.0 42-43 23448.0 44-45 23125.0 46-47 22825.0 48-49 22890.0 50-51 22896.0 52-53 22236.0 54-55 21239.0 56-57 20611.0 58-59 19458.0 60-61 18943.0 62-63 18308.0 64-65 17290.0 66-67 16176.0 68-69 15761.0 70-71 14887.0 72-73 13927.0 74-75 13032.0 76-77 12260.0 78-79 11576.0 80-81 10971.0 82-83 10143.0 84-85 9372.0 86-87 8661.0 88-89 7914.0 90-91 7517.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.78873624609417 #Duplication Level Percentage of deduplicated Percentage of total 1 99.79884975029334 99.58801095395602 2 0.1925146956783122 0.38421596381080364 3 0.007754491967291961 0.023214328609396602 4 3.364806795102638E-4 0.0013430792711822503 5 2.947936905727789E-4 0.0014708544917789862 6 0.0 0.0 7 2.497876909695204E-4 0.001744819860817484 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.775384687157398E-4 0.0 0.0 0.0 0.0 2 7.775384687157398E-4 0.0 0.0 0.0 0.0 3 7.775384687157398E-4 0.0 0.0 0.0 0.0 4 7.775384687157398E-4 0.0 0.0 0.0 0.0 5 7.775384687157398E-4 0.0 0.0 0.0 0.0 6 9.719230858946746E-4 0.0 0.0 0.0 0.0 7 0.0011663077030736096 0.0 0.0 0.0 0.0 8 0.005054000046652308 0.0 0.0 0.0 0.0 9 0.005442769281010178 0.0 0.0 0.0 0.0 10-11 0.005442769281010178 0.0 0.0 0.0 0.0 12-13 0.005442769281010178 0.0 9.719230858946747E-5 0.0 0.0 14-15 0.005442769281010178 0.0 1.9438461717893494E-4 0.0 0.0 16-17 0.005442769281010178 0.0 1.9438461717893494E-4 0.0 0.0 18-19 0.005442769281010178 0.0 1.9438461717893494E-4 0.0 0.0 20-21 0.005637153898189113 0.0 1.9438461717893494E-4 0.0 0.0 22-23 0.0058315385153680475 0.0 1.9438461717893494E-4 0.0 0.0 24-25 0.006025923132546983 0.0 1.9438461717893494E-4 0.0 1.9438461717893494E-4 26-27 0.006220307749725918 0.0 1.9438461717893494E-4 0.0 1.9438461717893494E-4 28-29 0.006220307749725918 0.0 1.9438461717893494E-4 0.0 1.9438461717893494E-4 30-31 0.006220307749725918 0.0 1.9438461717893494E-4 0.0 1.9438461717893494E-4 32-33 0.006317500058315386 0.0 1.9438461717893494E-4 0.0 1.9438461717893494E-4 34-35 0.006609076984083788 9.719230858946747E-5 1.9438461717893494E-4 0.0 1.9438461717893494E-4 36-37 0.006803461601262722 1.9438461717893494E-4 1.9438461717893494E-4 0.0 2.915769257684024E-4 38-39 0.007192230835620593 1.9438461717893494E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 40-41 0.007483807761388995 2.915769257684024E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 42-43 0.007678192378567929 3.887692343578699E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 44-45 0.007872576995746864 3.887692343578699E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 46-47 0.008164153921515267 3.887692343578699E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 48-49 0.008358538538694203 3.887692343578699E-4 1.9438461717893494E-4 0.0 3.887692343578699E-4 50-51 0.008650115464462604 5.831538515368048E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 52-53 0.008941692390231007 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 54-55 0.008941692390231007 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 56-57 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 58-59 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 60-61 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 62-63 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 5.831538515368048E-4 64-65 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 7.775384687157398E-4 66-67 0.009913615476125681 7.775384687157398E-4 1.9438461717893494E-4 0.0 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