##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-11C.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 432117 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.28052356190569 40.0 40.0 40.0 40.0 40.0 2 39.14473626355825 40.0 40.0 40.0 40.0 40.0 3 39.12364475361997 40.0 40.0 40.0 40.0 40.0 4 39.065614173939004 40.0 40.0 40.0 40.0 40.0 5 39.20876984705531 40.0 40.0 40.0 40.0 40.0 6 39.26540265715072 40.0 40.0 40.0 40.0 40.0 7 39.299671153877306 40.0 40.0 40.0 40.0 40.0 8 39.315203984106155 40.0 40.0 40.0 40.0 40.0 9 39.33097748989278 40.0 40.0 40.0 40.0 40.0 10-11 39.32641391104724 40.0 40.0 40.0 40.0 40.0 12-13 39.32879289636834 40.0 40.0 40.0 40.0 40.0 14-15 39.33671667627055 40.0 40.0 40.0 40.0 40.0 16-17 39.469055834415215 40.0 40.0 40.0 40.0 40.0 18-19 39.483991604125734 40.0 40.0 40.0 40.0 40.0 20-21 39.504555479187346 40.0 40.0 40.0 40.0 40.0 22-23 39.50453233730679 40.0 40.0 40.0 40.0 40.0 24-25 39.50894317974067 40.0 40.0 40.0 40.0 40.0 26-27 39.49599298338182 40.0 40.0 40.0 40.0 40.0 28-29 39.47332782556576 40.0 40.0 40.0 40.0 40.0 30-31 39.41566936569391 40.0 40.0 40.0 40.0 40.0 32-33 39.399420063292176 40.0 40.0 40.0 40.0 40.0 34-35 39.39024706968009 40.0 40.0 40.0 40.0 40.0 36-37 39.37448692598805 40.0 40.0 40.0 40.0 40.0 38-39 39.351533118789774 40.0 40.0 40.0 40.0 40.0 40-41 39.32244147078184 40.0 40.0 40.0 40.0 40.0 42-43 39.307905791663316 40.0 40.0 40.0 40.0 40.0 44-45 39.294395161887564 40.0 40.0 40.0 40.0 40.0 46-47 39.2779050612156 40.0 40.0 40.0 40.0 40.0 48-49 39.25116728742752 40.0 40.0 40.0 40.0 40.0 50-51 39.23407481998602 40.0 40.0 40.0 40.0 40.0 52-53 39.20829395368555 40.0 40.0 40.0 40.0 40.0 54-55 39.19901460239005 40.0 40.0 40.0 40.0 40.0 56-57 39.181385784858925 40.0 40.0 40.0 40.0 40.0 58-59 39.154429221668934 40.0 40.0 40.0 40.0 40.0 60-61 39.117135611706075 40.0 40.0 40.0 40.0 40.0 62-63 39.38938982748576 40.0 40.0 40.0 40.0 40.0 64-65 39.38346117591985 40.0 40.0 40.0 40.0 40.0 66-67 39.37612052874694 40.0 40.0 40.0 40.0 40.0 68-69 39.36367730616992 40.0 40.0 40.0 40.0 40.0 70-71 39.37049595443341 40.0 40.0 40.0 40.0 40.0 72-73 39.38025271023375 40.0 40.0 40.0 40.0 40.0 74-75 39.36099824790488 40.0 40.0 40.0 40.0 40.0 76-77 39.38172115224067 40.0 40.0 40.0 40.0 40.0 78-79 39.36439219141593 40.0 40.0 40.0 40.0 40.0 80-81 39.3366521468575 40.0 40.0 40.0 40.0 40.0 82-83 39.32522963119866 40.0 40.0 40.0 40.0 40.0 84-85 39.3240363264476 40.0 40.0 40.0 40.0 40.0 86-87 39.37916916011579 40.0 40.0 40.0 40.0 40.0 88-89 39.3541359439225 40.0 40.0 40.0 40.0 40.0 90-91 39.50140272063677 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3194929861681288 1101 2 0.09204129116420035 1101 3 0.1988608025912697 1101 4 0.3004190888112106 1101 5 0.24609978378160946 1101 6 0.14625698217422212 1101 7 0.17946746350134646 1101 8 0.01759040893826125 1101 9 0.006462170764685027 1101 10-11 -0.06637177931614247 1101 12-13 -0.024695980876749957 1101 14-15 -0.02842952086755446 1101 16-17 -0.09911615824816522 1101 18-19 0.01630976754556457 1101 20-21 0.0568553434235568 1101 22-23 0.03761533814815721 1101 24-25 -0.09840577763050362 1101 26-27 0.20974387270703687 1101 28-29 0.10790520065835807 1101 30-31 0.0021726002596764715 1101 32-33 0.2470777570505689 1101 34-35 0.02547731587360147 1101 36-37 0.15471955997368525 1101 38-39 0.04987505173426854 1101 40-41 0.12544346228029468 1101 42-43 0.14805654769864418 1101 44-45 0.05254633309513679 1101 46-47 0.08719803970394224 1101 48-49 -8.091668863130508E-4 1101 50-51 -0.19872484354364417 1101 52-53 -0.08926656326072901 1101 54-55 0.16840200083861845 1101 56-57 -0.02692422905888492 1101 58-59 -0.16893957641921276 1101 60-61 0.25775284575787794 1101 62-63 -0.09610636513770032 1101 64-65 -0.17934539585922948 1101 66-67 -0.3327914381614292 1101 68-69 -0.242053571726899 1101 70-71 -0.2151578025212686 1101 72-73 -0.09210883666550274 1101 74-75 -0.18858740977293564 1101 76-77 -0.31183632595163147 1101 78-79 -0.31926541787355234 1101 80-81 -0.4200438351308051 1101 82-83 -0.35518055612715216 1101 84-85 0.17984300706997658 1101 86-87 0.0203296808930844 1101 88-89 -0.3304730361630064 1101 90-91 0.00304554404988977 1102 1 0.28545379259700354 1102 2 0.06862101765510431 1102 3 0.1418054898416088 1102 4 0.01151453502578903 1102 5 0.014070083137781353 1102 6 0.08807755644932769 1102 7 0.08201927514662799 1102 8 -0.12404782594119723 1102 9 0.057355539813983114 1102 10-11 -0.1090843127998582 1102 12-13 -0.12582291353336217 1102 14-15 -0.010218138753728567 1102 16-17 0.20735501050976524 1102 18-19 0.07080856918097567 1102 20-21 0.08362163883216311 1102 22-23 -0.06644406313949247 1102 24-25 0.04772702936230644 1102 26-27 0.026487140717748048 1102 28-29 0.09013556862677063 1102 30-31 -0.042217614271088166 1102 32-33 0.08101731400552836 1102 34-35 -0.011651161900083196 1102 36-37 -0.15088461590895008 1102 38-39 -0.06059189059489967 1102 40-41 -0.15249655403633966 1102 42-43 -0.23168303779016952 1102 44-45 0.049385253611937685 1102 46-47 -0.20497307657877428 1102 48-49 0.08255369040251992 1102 50-51 0.06144555591659184 1102 52-53 -0.007935513005215 1102 54-55 0.026471983436344715 1102 56-57 0.07228751117929022 1102 58-59 -0.03571023295815223 1102 60-61 -0.07324109898583231 1102 62-63 -0.05494465756804345 1102 64-65 -0.04623040645558518 1102 66-67 0.1095479242799513 1102 68-69 0.22378511406317614 1102 70-71 0.27473354199222655 1102 72-73 0.31969527664499964 1102 74-75 0.18851670624684402 1102 76-77 0.2425950081280348 1102 78-79 0.10400958614573597 1102 80-81 -0.4738976311243661 1102 82-83 0.1832708654754711 1102 84-85 -0.47322106707520817 1102 86-87 0.23113809946114117 1102 88-89 -0.4274427331327004 1102 90-91 0.09828363928799178 1103 1 0.2777315217951326 1103 2 0.30695527841801606 1103 3 0.05669395223907259 1103 4 0.20182308516944403 1103 5 -0.16239894639233654 1103 6 -0.23104906986468876 1103 7 -0.04026515425705668 1103 8 -0.19148142009044733 1103 9 -0.13573151364759894 1103 10-11 0.08372261907381784 1103 12-13 -0.11984572451564901 1103 14-15 -0.0036693502796012467 1103 16-17 0.053761315516752006 1103 18-19 -9.176990316603906E-4 1103 20-21 0.022362075897554234 1103 22-23 -0.010737721413867973 1103 24-25 -0.03960276838233767 1103 26-27 0.07022017727361884 1103 28-29 -0.00701741182906801 1103 30-31 0.006383481231445387 1103 32-33 0.10108551629445373 1103 34-35 0.023697309677118028 1103 36-37 0.05249029739318445 1103 38-39 -0.0721877439085219 1103 40-41 -0.11633462208840939 1103 42-43 -0.12490312386870528 1103 44-45 0.03861305218488553 1103 46-47 -0.0500494807554972 1103 48-49 0.0021991052003045297 1103 50-51 -0.013066197814666225 1103 52-53 -0.011444095891469885 1103 54-55 -0.11648136239703177 1103 56-57 -0.2304968961925553 1103 58-59 0.03184505068300325 1103 60-61 0.06433485341023015 1103 62-63 -0.2599466203734835 1103 64-65 -0.12348549547962762 1103 66-67 0.042542107187109934 1103 68-69 0.20348243348652773 1103 70-71 -0.09558958674678308 1103 72-73 -0.12303857764775472 1103 74-75 -0.3011277993717414 1103 76-77 -0.31998898533604603 1103 78-79 -0.19407841782462043 1103 80-81 0.35734024195286906 1103 82-83 0.45433083319644396 1103 84-85 0.2845736606111373 1103 86-87 -0.6173929426674576 1103 88-89 0.6695269638369936 1103 90-91 0.6697122107165612 1104 1 0.29898249269110266 1104 2 0.06531412156692085 1104 3 -0.09345910665433621 1104 4 0.080158169922953 1104 5 0.044715779243887255 1104 6 0.13255306839089798 1104 7 -0.08626140774085655 1104 8 -0.03958486048885135 1104 9 -0.03265297387513044 1104 10-11 0.0016940176208990465 1104 12-13 0.05040328174377606 1104 14-15 0.11319271395944241 1104 16-17 -0.05705149574107793 1104 18-19 0.024931435157583337 1104 20-21 0.018012461971473215 1104 22-23 0.12601011977039178 1104 24-25 0.044441641541368426 1104 26-27 -0.10789651299914738 1104 28-29 0.016152506387221877 1104 30-31 0.0053819734871254354 1104 32-33 -0.03714012298951985 1104 34-35 0.18283055692606354 1104 36-37 -0.06490898144679846 1104 38-39 -0.0515889651823116 1104 40-41 0.06994729166783031 1104 42-43 -0.04450442392172249 1104 44-45 -0.0902253130599675 1104 46-47 0.11261820861646754 1104 48-49 0.08326164001788783 1104 50-51 -0.015520211220533042 1104 52-53 -0.2076677328113945 1104 54-55 -0.1487026518738972 1104 56-57 0.04914422856753475 1104 58-59 -0.19246783063434947 1104 60-61 0.07540236774609355 1104 62-63 0.22683740248876916 1104 64-65 0.14327126326639927 1104 66-67 -0.09967242510653307 1104 68-69 -0.01880844487443767 1104 70-71 -0.08814407072741659 1104 72-73 -0.01675476153810962 1104 74-75 -0.30429929416811774 1104 76-77 0.03945157410451827 1104 78-79 -0.32889297507540505 1104 80-81 -0.30116210893331896 1104 82-83 0.0993413721907217 1104 84-85 -0.36808438536588284 1104 86-87 0.12004695512531072 1104 88-89 0.3936648948714776 1104 90-91 -0.7302877892834374 1201 1 0.3198217242381176 1201 2 0.1200158404749061 1201 3 0.27977985526005256 1201 4 -0.03895878322838087 1201 5 0.08982194709019353 1201 6 0.19176776336899337 1201 7 0.1302097752722844 1201 8 0.04890182640026097 1201 9 0.01157351753032998 1201 10-11 -0.14360931908284869 1201 12-13 0.03588019445021473 1201 14-15 -0.006594950347938777 1201 16-17 -0.06711721869355358 1201 18-19 -0.16129539328865405 1201 20-21 0.0397768704649053 1201 22-23 -0.12327366856799671 1201 24-25 -0.06878046833392659 1201 26-27 0.1019248553864287 1201 28-29 -0.017313958054970158 1201 30-31 -0.0265206217268954 1201 32-33 0.08026449772960831 1201 34-35 0.002154200072389756 1201 36-37 0.028998455961790626 1201 38-39 0.04291672084894316 1201 40-41 -0.19631385957769254 1201 42-43 -0.08333889650400295 1201 44-45 0.051013975014754465 1201 46-47 -0.006790121718914577 1201 48-49 0.05384795212094673 1201 50-51 0.07250860545282478 1201 52-53 0.07482173912712398 1201 54-55 0.14166523067431314 1201 56-57 0.016585145700538817 1201 58-59 -0.0526223358569311 1201 60-61 -0.2017938868359721 1201 62-63 -0.23063295001423256 1201 64-65 0.019526787212363672 1201 66-67 0.01649836695141005 1201 68-69 0.02622154598306281 1201 70-71 -0.015116204023037483 1201 72-73 0.0034714654452017157 1201 74-75 0.09112809663305654 1201 76-77 -0.11901130594109333 1201 78-79 0.13422903275862552 1201 80-81 0.6062916859817804 1201 82-83 0.20582995136222593 1201 84-85 0.1270404834058283 1201 86-87 -0.05755237672933333 1201 88-89 -0.4661873218772925 1201 90-91 0.6697122107165612 1202 1 0.08942646732112536 1202 2 0.3064980539136428 1202 3 0.08852820835623021 1202 4 0.07753660544092611 1202 5 -0.0982017683248273 1202 6 -0.03201352780360622 1202 7 -0.057339631842339145 1202 8 0.006503934437148473 1202 9 0.17639565191768014 1202 10-11 -0.062957145169797 1202 12-13 0.1208472563737999 1202 14-15 -0.05264238117797504 1202 16-17 -0.04745318707300328 1202 18-19 0.09803925756773424 1202 20-21 -0.05281206234141678 1202 22-23 -0.027351402428337224 1202 24-25 0.009177370928256323 1202 26-27 -0.003160783612251805 1202 28-29 -0.10841408980727607 1202 30-31 0.011259351922639382 1202 32-33 0.04338788117906489 1202 34-35 -0.09017616143069063 1202 36-37 -0.13860180777425057 1202 38-39 -0.17144985278100222 1202 40-41 -0.27382379239170973 1202 42-43 -0.17311638844252286 1202 44-45 -0.2451461551778209 1202 46-47 0.051196034062378715 1202 48-49 -0.1673886187455622 1202 50-51 -0.10353505780211947 1202 52-53 -0.12479131346372441 1202 54-55 0.10608334183741164 1202 56-57 -0.15474581618277483 1202 58-59 -0.08515907736214245 1202 60-61 -0.13368623085293052 1202 62-63 -0.07705911049637848 1202 64-65 0.053616894787069214 1202 66-67 -0.20438469007269333 1202 68-69 -0.4838083538042639 1202 70-71 -0.138266645489189 1202 72-73 -0.01802398997397603 1202 74-75 -0.1842854228718238 1202 76-77 0.213810548463492 1202 78-79 0.31750370953974993 1202 80-81 0.15020902887113863 1202 82-83 -0.2169697096872909 1202 84-85 0.486759452960861 1202 86-87 0.27974921057225544 1202 88-89 -1.4439184143142612 1202 90-91 -0.9302877892834402 1203 1 0.2864310454767818 1203 2 0.15395390741266368 1203 3 0.10060145884675364 1203 4 0.23775897300141935 1203 5 0.05893361427818178 1203 6 -0.023772847620477933 1203 7 -0.07068168547215947 1203 8 0.010480389636626342 1203 9 -0.04832638863170047 1203 10-11 0.056539257707896695 1203 12-13 -0.022507877700761014 1203 14-15 0.04313917539494838 1203 16-17 0.023475524503197676 1203 18-19 0.039661232714891526 1203 20-21 0.11684130587310193 1203 22-23 -0.02347959078674222 1203 24-25 0.010799346075415883 1203 26-27 0.04364104765000576 1203 28-29 0.03702083498016151 1203 30-31 0.11456216421826326 1203 32-33 0.17437573464388123 1203 34-35 0.055762467248854364 1203 36-37 -0.06906104743947594 1203 38-39 0.1385306844535492 1203 40-41 0.017648312973584268 1203 42-43 0.08292790852678422 1203 44-45 0.013507900815156404 1203 46-47 0.011892742224212327 1203 48-49 0.11155282119945298 1203 50-51 -0.043042300599566374 1203 52-53 0.06281299317661393 1203 54-55 -0.12743747681073359 1203 56-57 -0.016801215437105554 1203 58-59 0.04635270835291294 1203 60-61 0.0735429843762816 1203 62-63 -0.05755576121614325 1203 64-65 -0.031405626113894414 1203 66-67 -0.12861196883583403 1203 68-69 -0.029977813909034978 1203 70-71 -0.31286300612519113 1203 72-73 0.05578988515283356 1203 74-75 0.13791977750441475 1203 76-77 0.12143537977252805 1203 78-79 0.18174588360130883 1203 80-81 0.17190254571028873 1203 82-83 0.3599076744054699 1203 84-85 0.5067958828333516 1203 86-87 0.5176947032347243 1203 88-89 -0.40383210952207804 1203 90-91 0.6697122107165612 1204 1 0.116679920581646 1204 2 0.10949195047644622 1204 3 -0.04474516138356677 1204 4 -0.13172776975984846 1204 5 0.08256276749271763 1204 6 -0.0457623231778399 1204 7 -0.11702482297629047 1204 8 -0.06906304275526765 1204 9 -0.04242863333769975 1204 10-11 -0.08797481301431986 1204 12-13 0.06216431387460375 1204 14-15 -0.023763486221334063 1204 16-17 0.01820694784508703 1204 18-19 -0.23804169328353453 1204 20-21 -0.05657049472175402 1204 22-23 -0.0319321926628362 1204 24-25 0.044050483681225217 1204 26-27 -0.09316399594762004 1204 28-29 -0.11672118906843565 1204 30-31 -0.03968828701085414 1204 32-33 -0.10070100074138821 1204 34-35 -0.05797462102017903 1204 36-37 -0.044360402819975775 1204 38-39 -0.22276703504959272 1204 40-41 -0.17787989630537737 1204 42-43 -0.20185692385835807 1204 44-45 -0.021750439605376926 1204 46-47 -0.2930464660724894 1204 48-49 -0.05329116574262116 1204 50-51 -0.25563121083031604 1204 52-53 -0.0769658823165571 1204 54-55 -0.0743104107691579 1204 56-57 -0.10550052804926935 1204 58-59 -0.18847956393396004 1204 60-61 -0.2653823073049182 1204 62-63 0.12292625106163513 1204 64-65 -0.26710927302839593 1204 66-67 0.13292928576724705 1204 68-69 -0.2489100578493435 1204 70-71 -0.1206512546650984 1204 72-73 -0.11513521758434564 1204 74-75 0.08696171988552237 1204 76-77 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-0.004490112699578219 2401 54-55 0.18606155482782327 2401 56-57 0.14337329381659458 2401 58-59 0.3131136485681978 2401 60-61 0.262928844648215 2401 62-63 0.27143230517526007 2401 64-65 0.1895201562754849 2401 66-67 0.018017230868458967 2401 68-69 0.2524022209783823 2401 70-71 0.3271674001100777 2401 72-73 -0.044117498726677695 2401 74-75 0.20254230627088532 2401 76-77 0.0479805665284303 2401 78-79 -0.20029059081997502 2401 80-81 0.06414116197907305 2401 82-83 0.2736671763423075 2401 84-85 0.21180257627378296 2401 86-87 -0.1657510408103562 2401 88-89 -0.19513469029833885 2401 90-91 0.2252677662721183 2402 1 0.394943889380329 2402 2 0.3486463379063096 2402 3 0.24919889213931157 2402 4 0.34382950562431347 2402 5 0.3260266833387888 2402 6 0.3004989165244041 2402 7 0.18756474332140272 2402 8 0.2702817704340035 2402 9 0.24744621518726007 2402 10-11 0.16061634186190332 2402 12-13 0.11662404442724039 2402 14-15 0.10198166912331175 2402 16-17 0.1414124141845292 2402 18-19 0.21043642812052354 2402 20-21 0.06945673514351824 2402 22-23 -0.0044905169161566505 2402 24-25 0.12278491612082121 2402 26-27 0.03015857714226655 2402 28-29 0.07989086172533888 2402 30-31 0.11597509727239697 2402 32-33 0.04194923422580388 2402 34-35 0.13243751605023135 2402 36-37 0.15764752110041513 2402 38-39 0.13527046043817137 2402 40-41 0.11135347602333923 2402 42-43 0.15916635082605524 2402 44-45 0.22753341864486032 2402 46-47 0.07148167383304127 2402 48-49 0.039382386908414446 2402 50-51 0.20139028544579674 2402 52-53 0.3030834531450566 2402 54-55 0.1743524840082742 2402 56-57 0.07088393206828414 2402 58-59 0.22888260764924695 2402 60-61 0.17349658314808636 2402 62-63 0.11605935700941927 2402 64-65 0.2033076540361023 2402 66-67 0.19266671629547716 2402 68-69 0.2839378324515991 2402 70-71 0.29684931240068124 2402 72-73 0.36045713080684294 2402 74-75 0.2855502759852868 2402 76-77 0.40225291166313326 2402 78-79 0.26509969371220166 2402 80-81 0.19968635610279506 2402 82-83 0.019910231266571543 2402 84-85 0.32191381406381936 2402 86-87 0.36151819416772213 2402 88-89 0.25573386038871604 2402 90-91 0.6697122107165612 2403 1 -0.47204442134077595 2403 2 -0.38955914731209873 2403 3 -0.15747792034652264 2403 4 0.057393602376571096 2403 5 -0.08230799917262743 2403 6 -0.01444457989757808 2403 7 0.034386619434478405 2403 8 0.07461425108679265 2403 9 0.15964386437937605 2403 10-11 0.09726757393132601 2403 12-13 0.06656737894785891 2403 14-15 0.14745976386799242 2403 16-17 0.10954374857561788 2403 18-19 -0.01315583947005905 2403 20-21 0.0758906955748202 2403 22-23 0.028440221677890065 2403 24-25 0.06584744242978502 2403 26-27 0.03867782721511759 2403 28-29 0.0082559816942549 2403 30-31 0.03904280442520047 2403 32-33 0.040638897019114495 2403 34-35 0.07197063465001463 2403 36-37 0.11085612738303041 2403 38-39 0.0533603936754119 2403 40-41 -0.016617799450649784 2403 42-43 0.06070144086088192 2403 44-45 0.07847565214288466 2403 46-47 0.190669798437618 2403 48-49 0.10079478956916432 2403 50-51 0.013358986546506912 2403 52-53 0.1200829526560554 2403 54-55 0.09324241019815815 2403 56-57 0.10679613985796976 2403 58-59 -0.009452357078870932 2403 60-61 -0.22671322970471408 2403 62-63 0.15191822293083135 2403 64-65 -0.06073848587946884 2403 66-67 0.15506272860840653 2403 68-69 -0.035986526672090235 2403 70-71 0.015978069822118357 2403 72-73 0.13310275610668754 2403 74-75 0.028679013837376033 2403 76-77 0.00901341052652782 2403 78-79 0.25292497504169376 2403 80-81 0.14402538411219012 2403 82-83 -0.28845660499329284 2403 84-85 0.0450334307260718 2403 86-87 0.3656927979183635 2403 88-89 0.19893872854287764 2403 90-91 0.6697122107165612 2404 1 -0.3409162715613192 2404 2 -0.28641295512174025 2404 3 -0.4119694949399175 2404 4 -0.4477614612896801 2404 5 -0.14979734653894639 2404 6 -0.3055787188590813 2404 7 -0.07327017547971337 2404 8 0.024257707593477562 2404 9 0.041399942463336004 2404 10-11 0.044061177983998334 2404 12-13 0.044729947807844894 2404 14-15 0.0223563568968288 2404 16-17 -0.052182882696641286 2404 18-19 -0.07799123054493862 2404 20-21 -0.017977879311786182 2404 22-23 0.049463498384341165 2404 24-25 -0.05354939702894512 2404 26-27 0.029626051984131152 2404 28-29 -0.0011464102353286876 2404 30-31 0.0909272132118275 2404 32-33 0.005884658325889802 2404 34-35 -0.08288377659985713 2404 36-37 0.06167963880467653 2404 38-39 -0.05048155935620713 2404 40-41 -0.10517886481283512 2404 42-43 -0.11239214797541308 2404 44-45 0.0089954898111273 2404 46-47 -0.04715249537795074 2404 48-49 -0.03945783112614265 2404 50-51 0.0469174794049394 2404 52-53 0.061881302455041975 2404 54-55 0.07838086465699945 2404 56-57 0.20580156605233668 2404 58-59 0.08373901700120001 2404 60-61 0.1790488177302194 2404 62-63 0.10357230335131362 2404 64-65 0.17667040357527952 2404 66-67 0.12192267391945677 2404 68-69 0.081393717425847 2404 70-71 -0.12019206110345948 2404 72-73 0.06133561717329172 2404 74-75 0.08262667926051392 2404 76-77 0.07842315075826178 2404 78-79 0.27914712236730566 2404 80-81 0.20028671962318612 2404 82-83 0.34542527667183975 2404 84-85 0.2701257761942131 2404 86-87 0.3448285756516185 2404 88-89 0.6695269638369936 2404 90-91 -1.2469544559501102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 277.0 32 1714.0 33 3180.0 34 4336.0 35 5298.0 36 7853.0 37 12449.0 38 36542.0 39 199847.0 40 160608.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.275854408116416 15.567491761395194 29.720766838227124 29.435886992261267 2 23.835896059172583 19.204243540727436 30.624496634975884 26.335363765124093 3 25.75333652695719 20.637752799627872 23.417594090407327 30.191316583007605 4 26.64694978443338 22.459426497916073 21.762161636778927 29.131462080871618 5 26.563639014433594 21.97043856177841 21.870697056584213 29.595225367203792 6 26.80084329012744 23.82688021994043 24.3251249083003 25.047151581631827 7 25.47712772235297 24.339704293050264 23.61976038896873 26.563407595628036 8 24.475547131911032 25.85295186257426 23.918059229329096 25.753441776185614 9 24.024049042273273 25.859200170324243 24.603984568994044 25.51276621840844 10-11 23.739751039648986 24.584198261119095 24.874050315076705 26.802000384155217 12-13 24.232673095481086 24.249913796494933 24.564874790855253 26.952538317168727 14-15 24.656169509646695 23.87952799820419 24.341787062300256 27.12251542984886 16-17 24.60826581689681 23.867147092107 24.11869933374526 27.40588775725093 18-19 24.83332060529903 24.136055744161883 23.87119692120421 27.15942672933488 20-21 24.796409305813008 24.139989863856318 23.928473075579067 27.135127754751608 22-23 24.50470590141096 23.85279912616259 24.197150308828398 27.445344663598053 24-25 24.705461715229905 24.08549073514812 24.18210808646732 27.026939463154655 26-27 24.851486981535093 24.10793835928695 24.076002564120365 26.964572095057587 28-29 24.891638144298884 23.9370355713846 24.199927334495055 26.971398949821463 30-31 24.940467236730964 24.12853059949319 24.167293428832604 26.763708734943243 32-33 25.08674972926001 24.020907903230217 24.22953520689766 26.662807160612118 34-35 24.95532413685489 23.834062141246317 24.470112683486835 26.740501038411956 36-37 25.01004200798696 24.08177174710207 24.37888560921947 26.5293006356915 38-39 25.076630233217706 24.097344939677555 24.40972903985863 26.416295787246113 40-41 25.00115544822725 23.926588596881444 24.47658195305414 26.59567400183716 42-43 25.12560880838982 24.21183838974509 24.351566166796186 26.3109866350689 44-45 25.14912570108875 24.086770042890134 24.42576707357308 26.338337182448036 46-47 25.200919605528284 24.01413138997133 24.39262540277124 26.392323601729146 48-49 25.25719543402628 24.351859838791608 24.299146036401275 26.091798690780838 50-51 25.329294349931033 24.213303262879982 24.287785889495243 26.16961649769374 52-53 25.301527923292475 24.10882504676041 24.310411832485247 26.27923519746187 54-55 25.492057795919994 24.26421846419155 24.21936133415073 26.024362405737726 56-57 25.47579610148854 24.258196856180685 24.18717023353773 26.078836808793042 58-59 25.441082089100398 24.126615080950824 24.249524709073516 26.182778120875263 60-61 25.75003607152564 24.32697244135441 23.9162603726608 26.006731114459146 62-63 25.846156145608358 24.064538789785594 23.98606990456689 26.103235160039162 64-65 25.760834993387682 24.192292883807358 23.828196929875954 26.218675192929002 66-67 25.92259437872052 24.178660236606135 23.7378494461608 26.160895938512546 68-69 26.05940994350523 23.94750561223947 23.765573299571237 26.22751114468407 70-71 25.93697072344544 24.283813210742803 23.620251633196226 26.158964432615534 72-73 26.51715314040818 24.15447881776132 23.315372292841243 26.01299574898926 74-75 26.527085677543806 23.848255907410383 23.302523710014466 26.322134705031345 76-77 26.333358479179243 23.925015087507546 23.416754677127336 26.324871756185882 78-79 26.860247958058636 24.018405598181943 23.0013725446077 26.11997389915172 80-81 26.778750443153616 23.815757179088603 22.933540592871363 26.471951784886414 82-83 27.635298350208153 23.39860547189529 22.105154957084856 26.860941220811707 84-85 28.629086809470124 22.949267192784667 21.38444193912063 27.037204058624575 86-87 28.80580785467571 22.18242292439555 21.085454778047843 27.9263144428809 88-89 28.785646637903824 21.545827633378934 19.914763758813006 29.75376196990424 90-91 26.813880126182966 22.94006309148265 18.826498422712934 31.41955835962145 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 2.5 9 3.0 10 2.5 11 5.0 12 11.0 13 17.5 14 24.0 15 31.0 16 47.5 17 71.0 18 91.0 19 126.0 20 183.5 21 261.0 22 367.0 23 497.5 24 660.5 25 859.0 26 1107.0 27 1433.0 28 1823.0 29 2268.5 30 2890.0 31 3663.0 32 4483.5 33 5265.5 34 6305.0 35 7763.0 36 9474.5 37 11182.0 38 12902.5 39 14942.0 40 17125.0 41 19352.5 42 21480.5 43 23417.5 44 25293.0 45 27157.5 46 28664.0 47 30015.0 48 31029.0 49 31638.0 50 32150.0 51 32143.5 52 31911.5 53 31619.0 54 30848.5 55 29817.0 56 28772.0 57 27661.5 58 26503.5 59 25104.5 60 23681.5 61 22327.5 62 21152.0 63 19853.0 64 18174.0 65 16515.5 66 14818.0 67 13232.5 68 11702.0 69 10090.5 70 8552.0 71 7160.0 72 5982.5 73 4864.0 74 3791.5 75 2999.0 76 2301.5 77 1636.5 78 1197.0 79 870.0 80 608.0 81 419.0 82 297.0 83 206.5 84 137.0 85 92.5 86 56.0 87 31.0 88 17.0 89 10.0 90 6.5 91 6.5 92 6.5 93 5.0 94 3.5 95 2.0 96 2.0 97 2.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.001157094027774885 2 0.005785470138874425 3 0.001388512833329862 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7490.0 32-33 16566.0 34-35 17506.0 36-37 18903.0 38-39 20237.0 40-41 21042.0 42-43 21822.0 44-45 21721.0 46-47 20849.0 48-49 20451.0 50-51 20578.0 52-53 19572.0 54-55 18602.0 56-57 17406.0 58-59 16452.0 60-61 15398.0 62-63 14665.0 64-65 13548.0 66-67 12380.0 68-69 11630.0 70-71 10587.0 72-73 10094.0 74-75 9417.0 76-77 8730.0 78-79 7903.0 80-81 7583.0 82-83 7098.0 84-85 6652.0 86-87 6261.0 88-89 5616.0 90-91 5358.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.82443295356222 #Duplication Level Percentage of deduplicated Percentage of total 1 99.8321535281315 99.65688116478692 2 0.1608065720386728 0.32104849737933333 3 0.006367174349258306 0.019067987067935308 4 3.5599622779311583E-4 0.001421484862922198 5 3.1672925276871695E-4 0.0015808659028721324 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0032398632777696783 0.0 0.0 0.0 0.0 2 0.0032398632777696783 0.0 0.0 0.0 0.0 3 0.003471282083324655 0.0 0.0 0.0 0.0 4 0.003471282083324655 0.0 0.0 0.0 0.0 5 0.003471282083324655 0.0 0.0 0.0 0.0 6 0.003702700888879632 0.0 0.0 0.0 0.0 7 0.003702700888879632 0.0 0.0 0.0 0.0 8 0.011108102666638897 0.0 0.0 0.0 0.0 9 0.011108102666638897 0.0 0.0 0.0 0.0 10-11 0.011108102666638897 0.0 0.0 0.0 0.0 12-13 0.011223812069416386 0.0 0.0 0.0 0.0 14-15 0.011339521472193874 0.0 0.0 0.0 1.157094027774885E-4 16-17 0.011339521472193874 0.0 0.0 0.0 2.31418805554977E-4 18-19 0.011339521472193874 2.31418805554977E-4 0.0 0.0 2.31418805554977E-4 20-21 0.011339521472193874 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 22-23 0.011339521472193874 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 24-25 0.011455230874971363 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 26-27 0.011918068486081316 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 28-29 0.01238090609719127 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 30-31 0.012728034305523736 4.62837611109954E-4 0.0 0.0 2.31418805554977E-4 32-33 0.012959453111078713 5.785470138874425E-4 0.0 0.0 2.31418805554977E-4 34-35 0.01330658131941118 6.94256416664931E-4 0.0 0.0 2.31418805554977E-4 36-37 0.01388512833329862 6.94256416664931E-4 0.0 0.0 2.31418805554977E-4 38-39 0.014116547138853597 6.94256416664931E-4 0.0 0.0 2.31418805554977E-4 40-41 0.014347965944408575 6.94256416664931E-4 0.0 0.0 3.4712820833246547E-4 42-43 0.014347965944408575 6.94256416664931E-4 0.0 0.0 5.785470138874425E-4 44-45 0.014463675347186063 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 46-47 0.014926512958296017 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 48-49 0.015273641166628483 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 50-51 0.015736478777738438 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 52-53 0.015967897583293415 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 54-55 0.016662153999958344 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 56-57 0.016662153999958344 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 58-59 0.016662153999958344 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 60-61 0.016662153999958344 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 62-63 0.016662153999958344 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 64-65 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 66-67 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 68-69 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 70-71 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 72-73 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 74-75 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 76-77 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 78-79 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 80 0.016893572805513322 6.94256416664931E-4 0.0 0.0 6.94256416664931E-4 >>END_MODULE