##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-11A.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 506856 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.26383035812933 40.0 40.0 40.0 40.0 40.0 2 39.16620894297394 40.0 40.0 40.0 40.0 40.0 3 39.12047603264043 40.0 40.0 40.0 40.0 40.0 4 39.06397083195227 40.0 40.0 40.0 40.0 40.0 5 39.20978739523652 40.0 40.0 40.0 40.0 40.0 6 39.27131966475685 40.0 40.0 40.0 40.0 40.0 7 39.286866486733906 40.0 40.0 40.0 40.0 40.0 8 39.31014726076045 40.0 40.0 40.0 40.0 40.0 9 39.32661742191076 40.0 40.0 40.0 40.0 40.0 10-11 39.32152327288224 40.0 40.0 40.0 40.0 40.0 12-13 39.3158451315561 40.0 40.0 40.0 40.0 40.0 14-15 39.32796691762552 40.0 40.0 40.0 40.0 40.0 16-17 39.461140836845175 40.0 40.0 40.0 40.0 40.0 18-19 39.48033366478842 40.0 40.0 40.0 40.0 40.0 20-21 39.49278296005177 40.0 40.0 40.0 40.0 40.0 22-23 39.48948024685512 40.0 40.0 40.0 40.0 40.0 24-25 39.48358902725823 40.0 40.0 40.0 40.0 40.0 26-27 39.47181447985227 40.0 40.0 40.0 40.0 40.0 28-29 39.47096216676926 40.0 40.0 40.0 40.0 40.0 30-31 39.459469025208406 40.0 40.0 40.0 40.0 40.0 32-33 39.45563995567207 40.0 40.0 40.0 40.0 40.0 34-35 39.45164440739347 40.0 40.0 40.0 40.0 40.0 36-37 39.4407219632572 40.0 40.0 40.0 40.0 40.0 38-39 39.42606141085646 40.0 40.0 40.0 40.0 40.0 40-41 39.41893443123486 40.0 40.0 40.0 40.0 40.0 42-43 39.41441775837646 40.0 40.0 40.0 40.0 40.0 44-45 39.38694989075583 40.0 40.0 40.0 40.0 40.0 46-47 39.39293838633985 40.0 40.0 40.0 40.0 40.0 48-49 39.39088625143552 40.0 40.0 40.0 40.0 40.0 50-51 39.391873968255005 40.0 40.0 40.0 40.0 40.0 52-53 39.37867733295539 40.0 40.0 40.0 40.0 40.0 54-55 39.35984403235604 40.0 40.0 40.0 40.0 40.0 56-57 39.3644966520111 40.0 40.0 40.0 40.0 40.0 58-59 39.33544092320828 40.0 40.0 40.0 40.0 40.0 60-61 39.314718686688664 40.0 40.0 40.0 40.0 40.0 62-63 39.360995508965374 40.0 40.0 40.0 40.0 40.0 64-65 39.340012717721535 40.0 40.0 40.0 40.0 40.0 66-67 39.36137419867103 40.0 40.0 40.0 40.0 40.0 68-69 39.351188726463604 40.0 40.0 40.0 40.0 40.0 70-71 39.34073718847938 40.0 40.0 40.0 40.0 40.0 72-73 39.34031804579749 40.0 40.0 40.0 40.0 40.0 74-75 39.34180911904916 40.0 40.0 40.0 40.0 40.0 76-77 39.31297794741589 40.0 40.0 40.0 40.0 40.0 78-79 39.322444211032916 40.0 40.0 40.0 40.0 40.0 80-81 39.317230266249126 40.0 40.0 40.0 40.0 40.0 82-83 39.286923883160725 40.0 40.0 40.0 40.0 40.0 84-85 39.278303474024106 40.0 40.0 40.0 40.0 40.0 86-87 39.35383421549449 40.0 40.0 40.0 40.0 40.0 88-89 39.31813018855706 40.0 40.0 40.0 40.0 40.0 90-91 39.440678053038496 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14699166569393896 1101 2 -0.055532039944942824 1101 3 0.006990030952593429 1101 4 -0.02344827591390697 1101 5 -0.03056427024738184 1101 6 -0.16109885464035045 1101 7 0.05333190517548303 1101 8 0.17688614945108583 1101 9 0.06147259938859406 1101 10-11 0.009816981413742099 1101 12-13 0.05080186347246496 1101 14-15 0.0021796192941252457 1101 16-17 0.004662305763694974 1101 18-19 0.07223962705852216 1101 20-21 -0.02788222593319034 1101 22-23 0.09885797494929704 1101 24-25 0.008643848856365821 1101 26-27 0.05499289804946983 1101 28-29 0.044031192135783215 1101 30-31 0.07304348590805887 1101 32-33 -0.005071889491347292 1101 34-35 0.10082629744275806 1101 36-37 0.037770085766609895 1101 38-39 0.0803697775987331 1101 40-41 0.06704087489909227 1101 42-43 0.031848987799513395 1101 44-45 0.1486550103364337 1101 46-47 0.275855018157003 1101 48-49 0.09053912532271369 1101 50-51 0.11570442799978053 1101 52-53 0.0528250846683207 1101 54-55 0.28154056547656836 1101 56-57 0.2216181476297976 1101 58-59 0.132485008425121 1101 60-61 0.21120192546713668 1101 62-63 0.09852608632921545 1101 64-65 -0.11553273071292125 1101 66-67 0.12777302668850865 1101 68-69 0.11248404077414165 1101 70-71 0.2143861264383915 1101 72-73 -0.4030116300273008 1101 74-75 -0.0801192635552539 1101 76-77 -0.1953980320170956 1101 78-79 -0.26202929049276236 1101 80-81 0.09704680760316364 1101 82-83 -0.34918674296905294 1101 84-85 0.2310974318111434 1101 86-87 0.25064026562529307 1101 88-89 -0.20421501067092862 1101 90-91 -2.892883995089875 1102 1 0.3042859173834742 1102 2 0.22635211981532422 1102 3 0.2234998403827575 1102 4 0.07932520975043644 1102 5 -0.01941413992320662 1102 6 -0.0014509640190070172 1102 7 0.1359777199746901 1102 8 0.019298473279341977 1102 9 0.08756864908346529 1102 10-11 0.00569343097750874 1102 12-13 -0.055870421960882766 1102 14-15 0.0086380794399048 1102 16-17 0.024003349293529652 1102 18-19 0.04940870511256179 1102 20-21 0.10645936386562482 1102 22-23 -0.022963129762665346 1102 24-25 0.005094661236384468 1102 26-27 -0.02990978803315869 1102 28-29 0.13257359041269012 1102 30-31 0.032668881074883416 1102 32-33 0.08228315955862797 1102 34-35 0.04207184436726408 1102 36-37 0.03642441706066535 1102 38-39 0.0025914170457070895 1102 40-41 -0.04923105825474039 1102 42-43 0.06325630200556276 1102 44-45 0.21659815445091368 1102 46-47 0.06866967800456081 1102 48-49 0.07429112997652254 1102 50-51 -0.09988410114537061 1102 52-53 0.09303779418397795 1102 54-55 -0.017750994917122398 1102 56-57 0.1136360500416842 1102 58-59 -0.002397614043175622 1102 60-61 0.1463305530367336 1102 62-63 0.16230960768470482 1102 64-65 -0.06576048460155448 1102 66-67 0.23007665505319608 1102 68-69 0.1108725407458806 1102 70-71 0.14310305866799666 1102 72-73 -0.126803177985515 1102 74-75 -0.05132389923203817 1102 76-77 0.23651812058871258 1102 78-79 -0.08522579454033519 1102 80-81 0.19323006354460404 1102 82-83 -0.058178483657847835 1102 84-85 -0.08635363379545424 1102 86-87 0.015552546327043615 1102 88-89 -0.40721459173502694 1102 90-91 0.5737826715767937 1103 1 0.3127641647997734 1103 2 0.13387114945824408 1103 3 3.115242740889812E-4 1103 4 0.06891614978385263 1103 5 -0.11054601689579613 1103 6 -0.03785173139322495 1103 7 0.031064049574297314 1103 8 0.08002005258499167 1103 9 0.10542247667180504 1103 10-11 -0.07178351685342221 1103 12-13 0.014393827064431264 1103 14-15 0.09025204069988035 1103 16-17 0.03382450159255512 1103 18-19 0.03596109077998477 1103 20-21 -0.019591134308761582 1103 22-23 -0.005910713152729841 1103 24-25 -0.05339596318344775 1103 26-27 -0.045662619272711424 1103 28-29 0.04653794464253025 1103 30-31 -0.08165750050812903 1103 32-33 0.03055963547603824 1103 34-35 0.021580847568664296 1103 36-37 -0.025342978129302196 1103 38-39 -0.09766461451680186 1103 40-41 -0.047476822284309605 1103 42-43 -0.040174160615215726 1103 44-45 -0.15557593867772823 1103 46-47 -0.012860809908289639 1103 48-49 0.059693201420977005 1103 50-51 -0.21317828266416683 1103 52-53 0.0325361547818801 1103 54-55 -0.07021958689215779 1103 56-57 -0.23000672085209573 1103 58-59 -0.09067151886445401 1103 60-61 -0.22838868344639707 1103 62-63 0.03675610638832438 1103 64-65 -0.00801097996938438 1103 66-67 -0.10763891378585555 1103 68-69 -0.335767148430584 1103 70-71 -0.06328394651974634 1103 72-73 -0.17059080340357724 1103 74-75 -0.11990557638507227 1103 76-77 -0.621280518276933 1103 78-79 0.017517525009715484 1103 80-81 0.11489640442962212 1103 82-83 0.40176086015246426 1103 84-85 0.4783522485574849 1103 86-87 -0.009188794203765838 1103 88-89 0.7308499243940076 1103 90-91 0.5737826715767937 1104 1 0.27578784104041176 1104 2 0.19041394166188752 1104 3 0.2843653756596112 1104 4 0.28801292997788863 1104 5 0.20439506861885803 1104 6 0.07336788855457144 1104 7 0.014284901872457567 1104 8 -0.06935011174776662 1104 9 0.10413490306503093 1104 10-11 0.036315989625130385 1104 12-13 -0.05243211507323764 1104 14-15 -0.025577267149380134 1104 16-17 0.062014516066859926 1104 18-19 0.03984706349388745 1104 20-21 -0.044634876933557166 1104 22-23 0.009579407698993236 1104 24-25 -0.03401405664443047 1104 26-27 -0.0668145762102057 1104 28-29 -0.07268759291218174 1104 30-31 -0.10897847546002737 1104 32-33 -0.020731686495579993 1104 34-35 -0.0216523208905528 1104 36-37 -0.1052296227922156 1104 38-39 -0.11010512417647789 1104 40-41 0.005923381146935469 1104 42-43 -0.03396051479388973 1104 44-45 -0.16926400589202473 1104 46-47 -0.07385696991851631 1104 48-49 0.1315659050094382 1104 50-51 -0.14227789554833237 1104 52-53 -0.01924230280285144 1104 54-55 -0.022517426215685532 1104 56-57 -0.2890682643565583 1104 58-59 -0.04044052736166748 1104 60-61 -0.11630786504366597 1104 62-63 0.0667833103278781 1104 64-65 -0.2506432807827821 1104 66-67 -0.009312844336299975 1104 68-69 -0.158674487012469 1104 70-71 -0.10433863168964308 1104 72-73 -0.16496850960454879 1104 74-75 -0.05788040841066078 1104 76-77 -0.04455944055520433 1104 78-79 0.1385447987954791 1104 80-81 -0.07278790528682322 1104 82-83 -0.18773344184184282 1104 84-85 0.26588612389623734 1104 86-87 0.6061958211808474 1104 88-89 0.7308499243940076 1104 90-91 0.5737826715767937 1201 1 0.1991498936454903 1201 2 0.2797581184286173 1201 3 0.07464536229790042 1201 4 0.06014396156768953 1201 5 0.14218608672089061 1201 6 0.07451357810771952 1201 7 -0.025366040975427495 1201 8 0.028176361314173448 1201 9 -0.07224526418043098 1201 10-11 0.12375115066944886 1201 12-13 0.0757814705919202 1201 14-15 -0.09065887652106142 1201 16-17 -0.08091795897971821 1201 18-19 0.009759407038281154 1201 20-21 0.0033482262464374912 1201 22-23 -0.01742973330695463 1201 24-25 0.010868121510299034 1201 26-27 -0.03792583204982947 1201 28-29 0.06913560814162878 1201 30-31 0.016963339477491957 1201 32-33 0.10548527252625206 1201 34-35 0.041554941440573145 1201 36-37 0.05789275044345743 1201 38-39 0.008029807078045792 1201 40-41 0.060414169478058 1201 42-43 0.08996885863417248 1201 44-45 0.029272429566709945 1201 46-47 -0.012525523416350381 1201 48-49 -0.002775005477609227 1201 50-51 -0.02553780669141048 1201 52-53 0.0037637871523870103 1201 54-55 0.028682698964445308 1201 56-57 -0.00898542515147227 1201 58-59 -0.09956746553550033 1201 60-61 0.04329335200208817 1201 62-63 -0.3411948586663698 1201 64-65 0.09318429195759137 1201 66-67 -0.01524268725862754 1201 68-69 -0.06301473094132604 1201 70-71 -0.09868792773676205 1201 72-73 -0.2851491194836342 1201 74-75 -0.08527082398975949 1201 76-77 0.1713144431699618 1201 78-79 -0.5289946183091558 1201 80-81 -0.6183273001689855 1201 82-83 0.06746229486797972 1201 84-85 -0.42170016234398133 1201 86-87 -0.14335372836870874 1201 88-89 0.003577197121281017 1201 90-91 0.5737826715767937 1202 1 0.043607789260420304 1202 2 0.09206189488303096 1202 3 0.11301796676776377 1202 4 -0.101147828790765 1202 5 0.07647972332143382 1202 6 0.07716091553431426 1202 7 0.018491515725166607 1202 8 0.07020008531821276 1202 9 0.14596703849296233 1202 10-11 0.054425380632068254 1202 12-13 -0.03931841813717796 1202 14-15 0.029546788505264487 1202 16-17 -0.06474713953226541 1202 18-19 -0.07376960405709809 1202 20-21 -0.03584615588293616 1202 22-23 -0.026778143594626158 1202 24-25 0.012107682107441065 1202 26-27 -9.648328104816528E-5 1202 28-29 -0.12549746675458806 1202 30-31 0.010888251612087174 1202 32-33 0.030507916630966747 1202 34-35 -0.05556091814332831 1202 36-37 -0.032204399514924376 1202 38-39 -0.035786338581530686 1202 40-41 0.15753093234150128 1202 42-43 0.03591668788784119 1202 44-45 0.10241106059478966 1202 46-47 -0.031107606168426116 1202 48-49 -0.008763559370308371 1202 50-51 0.027078156043010893 1202 52-53 0.12556871055654995 1202 54-55 -0.01034438714867747 1202 56-57 0.04115950657698164 1202 58-59 0.12846435468215134 1202 60-61 -0.13912151766616887 1202 62-63 -0.15411526087228822 1202 64-65 -0.3721472668475485 1202 66-67 -0.06016906450215487 1202 68-69 -0.11360941012755887 1202 70-71 0.1574941574174602 1202 72-73 0.18517388004417512 1202 74-75 0.05468989045292716 1202 76-77 0.2757578696472862 1202 78-79 0.28770212058707045 1202 80-81 0.3458945245179521 1202 82-83 0.6479147063063095 1202 84-85 0.14450211935852053 1202 86-87 0.17090170353378653 1202 88-89 0.44513563867972294 1202 90-91 0.5737826715767937 1203 1 0.14825876728466625 1203 2 0.28468473716503695 1203 3 0.011253656575441084 1203 4 0.09190933782558375 1203 5 -0.015427543136539157 1203 6 -0.025382000476895428 1203 7 -0.13553472382059084 1203 8 0.0028480336653942118 1203 9 -0.12619541999463024 1203 10-11 0.017762050847487387 1203 12-13 -0.078031546914346 1203 14-15 0.002907346559069879 1203 16-17 -0.02714365646297523 1203 18-19 0.07699125802374596 1203 20-21 0.09667043583629464 1203 22-23 -0.05140486301580438 1203 24-25 0.02261621234329425 1203 26-27 0.03352922448223694 1203 28-29 -0.011267518502741325 1203 30-31 -0.13513476757763243 1203 32-33 -0.010603978072531106 1203 34-35 0.008847848454195173 1203 36-37 -0.27146162195684553 1203 38-39 -0.02324392474727688 1203 40-41 -0.1479224696525705 1203 42-43 -0.14007364018606694 1203 44-45 0.030809126592522773 1203 46-47 -0.13942854507846647 1203 48-49 -0.21308923602934726 1203 50-51 -0.018337099100314447 1203 52-53 -0.18123470990142465 1203 54-55 -0.11672535334503209 1203 56-57 -0.03851395351281184 1203 58-59 -0.043593486168177265 1203 60-61 0.048106406682073555 1203 62-63 -0.006144105787143417 1203 64-65 -0.10017526122535969 1203 66-67 -0.06939973581457082 1203 68-69 -0.007945953854466836 1203 70-71 0.07285911305166337 1203 72-73 -0.00846439206006977 1203 74-75 0.4958436498181058 1203 76-77 0.10995426019651688 1203 78-79 0.36445492065762863 1203 80-81 0.19249508987252284 1203 82-83 0.45743851583011974 1203 84-85 0.008312978518219438 1203 86-87 0.43598305522340297 1203 88-89 -0.06544637190228997 1203 90-91 -0.6706617728676463 1204 1 0.18055987619738545 1204 2 0.18344085305638202 1204 3 -0.12385577761833133 1204 4 0.023610744288873775 1204 5 0.09598520859277926 1204 6 -0.10522985090404546 1204 7 -0.005011249534177864 1204 8 -0.024974478949829404 1204 9 -0.0752743935985194 1204 10-11 -0.07883452271959612 1204 12-13 -0.04413532151211541 1204 14-15 -0.16909682528405767 1204 16-17 -0.04054110120906529 1204 18-19 -0.04990486001486971 1204 20-21 -0.044942165583968574 1204 22-23 -0.12175103396934617 1204 24-25 -0.10324461374299432 1204 26-27 -0.2056538918232249 1204 28-29 -0.14692143957514503 1204 30-31 -0.09525685533971284 1204 32-33 -0.08909540192887277 1204 34-35 -0.06489804281909528 1204 36-37 0.013941474977322343 1204 38-39 -0.23776566089793505 1204 40-41 -0.02076281191217788 1204 42-43 -0.11203895876065673 1204 44-45 -0.1969610663265442 1204 46-47 -0.18434787940869768 1204 48-49 -0.23778648097632527 1204 50-51 -0.16689132626011371 1204 52-53 0.03067592061803026 1204 54-55 -0.07574790683077026 1204 56-57 0.02475893476843538 1204 58-59 -0.3121609242513159 1204 60-61 -0.17295356084095204 1204 62-63 -0.07726950253533005 1204 64-65 -0.07757631001820897 1204 66-67 -0.06991683294029372 1204 68-69 0.10437054014599312 1204 70-71 -0.01126336844431819 1204 72-73 -0.09496105428618762 1204 74-75 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0.2846712372328568 2401 56-57 0.12621533688934505 2401 58-59 0.1243325225689702 2401 60-61 -0.07754092580206873 2401 62-63 -0.011068849083855525 2401 64-65 0.2249984907321405 2401 66-67 0.15004062947262042 2401 68-69 0.0278490745931137 2401 70-71 0.06477753685731358 2401 72-73 0.14772547963137583 2401 74-75 0.1916194417672088 2401 76-77 -0.024033849422643527 2401 78-79 -0.04720195314299502 2401 80-81 -0.005808726038438294 2401 82-83 0.15538004580150755 2401 84-85 0.21238002633526065 2401 86-87 -0.5245734095883847 2401 88-89 0.4549878554284916 2401 90-91 0.5737826715767937 2402 1 0.3442597024288858 2402 2 0.3360456219643879 2402 3 0.25051089328308507 2402 4 0.3106022238188615 2402 5 0.27431718895787327 2402 6 0.11683637922400436 2402 7 0.1570477886884305 2402 8 0.02298304143621266 2402 9 0.09506323119216376 2402 10-11 0.12587854393903086 2402 12-13 0.13597238480836182 2402 14-15 0.16062691798070716 2402 16-17 0.09283931685453695 2402 18-19 0.04855390459951536 2402 20-21 -0.009140544462447053 2402 22-23 0.021725326409708146 2402 24-25 -0.005748358629887207 2402 26-27 0.019329294765086047 2402 28-29 0.07828209780875994 2402 30-31 0.13426469633182592 2402 32-33 0.018560160697219885 2402 34-35 -0.020566832940673407 2402 36-37 0.00969649900950742 2402 38-39 0.09165162663938986 2402 40-41 0.043232418789727944 2402 42-43 -6.334808313539497E-4 2402 44-45 0.018118230685594483 2402 46-47 0.1477492673686598 2402 48-49 0.11427223729066327 2402 50-51 0.09603382612424838 2402 52-53 0.17972276125977515 2402 54-55 0.06939122613414384 2402 56-57 0.014796236776497551 2402 58-59 0.16379623007492228 2402 60-61 0.3105061748237006 2402 62-63 0.08244747269888819 2402 64-65 0.12922569209436574 2402 66-67 0.21321731060764648 2402 68-69 0.3291432432182617 2402 70-71 -0.005789433758828011 2402 72-73 0.18640386019254152 2402 74-75 0.12256526709278859 2402 76-77 0.19513414699676446 2402 78-79 0.14629206784539406 2402 80-81 0.1717137689914381 2402 82-83 0.11073034103478108 2402 84-85 -0.21053664033140507 2402 86-87 0.3043090287280137 2402 88-89 0.4641832577273419 2402 90-91 0.5737826715767937 2403 1 -0.11491706486165043 2403 2 -0.0644948409869528 2403 3 0.13549811985672022 2403 4 0.017749494656797538 2403 5 0.005842545037324953 2403 6 0.19291749937600144 2403 7 0.1424328787649074 2403 8 0.12834796229903844 2403 9 -0.014531826450813412 2403 10-11 0.06625913356545965 2403 12-13 0.11146507265052463 2403 14-15 0.16101296294594647 2403 16-17 0.1231757444091457 2403 18-19 0.11243412269855924 2403 20-21 0.08587518155358964 2403 22-23 0.05157388146058395 2403 24-25 0.10534875744247074 2403 26-27 0.09535725194518108 2403 28-29 0.12576317485785893 2403 30-31 0.0994683043800535 2403 32-33 0.05647198316382429 2403 34-35 0.10328255973050204 2403 36-37 0.05294809466984418 2403 38-39 0.11088770967145933 2403 40-41 0.05413970212745767 2403 42-43 0.09585351006398923 2403 44-45 0.14883338184806405 2403 46-47 0.04804980748249221 2403 48-49 -0.025626669703996185 2403 50-51 0.06780200635136424 2403 52-53 0.12358912348238249 2403 54-55 0.1992404551579483 2403 56-57 0.07239815450723341 2403 58-59 0.09277057793955379 2403 60-61 0.19166499738952325 2403 62-63 0.2779294759941777 2403 64-65 0.2869700216052493 2403 66-67 0.1605550952873216 2403 68-69 0.17672140288702565 2403 70-71 0.18655496382977077 2403 72-73 0.3321573459030631 2403 74-75 0.3036719024069754 2403 76-77 0.32307722716168286 2403 78-79 0.25351744307931057 2403 80-81 0.5170618584759836 2403 82-83 0.011090381981986752 2403 84-85 0.3556705000008691 2403 86-87 0.3728624878475131 2403 88-89 -0.45596326241918206 2403 90-91 0.10319443628267777 2404 1 -0.39916984247101794 2404 2 -0.07209041919762171 2404 3 -0.22093441458843444 2404 4 -0.1899458862802561 2404 5 0.019869336317491104 2404 6 -0.042284259141815994 2404 7 -0.05749592240536572 2404 8 0.12891071287461386 2404 9 0.052032861106027894 2404 10-11 0.15054035961552614 2404 12-13 0.11232008043109687 2404 14-15 0.10685288372559398 2404 16-17 0.031318372143424256 2404 18-19 0.03579200252799808 2404 20-21 0.08365837956251454 2404 22-23 -0.003087543231110601 2404 24-25 0.10023581091465417 2404 26-27 0.10131432433635013 2404 28-29 0.013532521740735604 2404 30-31 0.03044197169496954 2404 32-33 0.07711450479084192 2404 34-35 0.025673873704917582 2404 36-37 0.12738186475301205 2404 38-39 0.1037349501617797 2404 40-41 0.15850945061916377 2404 42-43 0.08227555711560797 2404 44-45 0.0872457719359474 2404 46-47 0.15189912732910926 2404 48-49 -0.018859136400450893 2404 50-51 0.17430293359382176 2404 52-53 0.1639853318408271 2404 54-55 0.011709556364806417 2404 56-57 0.055803143118204446 2404 58-59 0.06127166460355227 2404 60-61 0.18212457721240582 2404 62-63 0.1443017241353104 2404 64-65 0.03840610407900158 2404 66-67 -0.02346300860521211 2404 68-69 0.011331578295852296 2404 70-71 -0.06933417231300609 2404 72-73 -0.10663395516177587 2404 74-75 -0.08122683054150315 2404 76-77 0.17412567230822162 2404 78-79 0.16030848875949744 2404 80-81 0.25449335288934805 2404 82-83 0.4918863375119855 2404 84-85 0.015725985931226205 2404 86-87 -0.1701199682928376 2404 88-89 -0.06687087332679198 2404 90-91 0.19283029062441415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 18.0 31 319.0 32 2008.0 33 3711.0 34 4964.0 35 6141.0 36 9102.0 37 14141.0 38 37531.0 39 207967.0 40 220954.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.476671507039583 13.88503062061999 30.206886482732497 30.43141138960793 2 24.275290830742033 17.717195712910204 29.937808785692866 28.0697046706549 3 26.3259242306463 19.197836063245035 23.004454931794953 31.47178477431371 4 26.87508878261281 20.92172135675616 21.274089682276625 30.929100178354403 5 26.982219802073963 20.608022791483183 21.149596729643132 31.26016067679972 6 27.475851129314837 22.622401628865003 23.90915763056963 25.992589611250533 7 25.987065359786605 22.939848793345643 23.172261944220843 27.900823902646906 8 24.747265495525355 24.08909039253753 23.6854254462806 27.478218665656517 9 24.69873100052086 24.16919203876446 24.104084789368184 27.027992171346497 10-11 24.324462963839828 22.850375649099547 24.221179190933913 28.603982196126708 12-13 24.89839323200278 22.62713670154837 23.858946130656438 28.615523935792414 14-15 25.3753531575043 22.225937938980696 23.595163123253943 28.803545780261057 16-17 25.343884653629434 22.016213678046626 23.43722871979418 29.202672948529763 18-19 25.570576258345568 22.373514370945593 23.204125037485994 28.851784333222845 20-21 25.567912779961173 22.386042584086997 23.149967643669996 28.896076992281834 22-23 25.351085910002052 22.076191265369257 23.351208232713038 29.221514591915653 24-25 25.49698533705826 22.514382783275725 23.373502533263885 28.615129346402135 26-27 25.670900610824376 22.573571191817795 23.248910933282826 28.506617264075 28-29 25.660148049939234 22.343426929936708 23.368175576495098 28.62824944362896 30-31 25.868914785041436 22.584533795268257 23.298349523087044 28.24820189660326 32-33 25.937662332360745 22.425211025690484 23.4769971536663 28.160129488282475 34-35 25.66990774618721 22.340594467346357 23.61034513097724 28.379152655489197 36-37 25.877950032321355 22.704560146069838 23.607516699366048 27.80997312224276 38-39 25.915280608860318 22.625408580491865 23.558841621536867 27.900469189110954 40-41 25.883603679480604 22.518410966619346 23.670283194963126 27.927702158936924 42-43 25.965794964800402 22.768858318498523 23.689870261566277 27.575476455134794 44-45 25.96821186851561 22.73034160988776 23.687791792723136 27.613654728873488 46-47 25.89203483935479 22.650843354472016 23.788089098229086 27.66903270794411 48-49 26.161973921130215 22.98135193522914 23.66723088739749 27.189443256243152 50-51 26.06101333122511 22.933857754148097 23.656846952792243 27.34828196183455 52-53 26.132620068853875 22.774575553819908 23.75611416304144 27.336690214284783 54-55 26.240901426985236 23.040042187866632 23.622302480230484 27.09675390491764 56-57 26.256762637468906 23.04615516861599 23.661306700047817 27.035775493867288 58-59 26.324888762378464 22.916598001468184 23.627919519393547 27.130593716759805 60-61 26.47058823529412 23.094836020532615 23.535777947542652 26.898797796630614 62-63 26.73561211975291 22.924676653599885 23.4803283498988 26.859382876748406 64-65 26.444466153852687 23.112307960226477 23.370130780306926 27.07309510561391 66-67 26.774726677877407 23.238523843711302 23.398011939924544 26.58873753848674 68-69 26.948263673876472 23.10626195316824 23.015294575385102 26.930179797570187 70-71 26.771267159052304 23.11800898373114 23.253268241126364 26.857455616090192 72-73 27.108328606954323 23.12653607317889 23.10108634003752 26.664048979829268 74-75 27.13884338208456 23.376821804785713 22.697756538125272 26.78657827500446 76-77 27.01192175468929 23.29165075088961 22.862427373796265 26.834000120624836 78-79 27.844148955407412 23.088329254620195 22.176947836674664 26.890573953297732 80-81 27.533983451536642 22.861997635933808 22.15868794326241 27.44533096926714 82-83 28.196800866043265 22.989342441719394 21.932918976426453 26.880937715810887 84-85 29.245678494809262 22.607451976425914 20.619929475008014 27.52694005375681 86-87 29.52792993963923 21.981089285091237 20.219113080492658 28.271867694776876 88-89 29.58495312142694 21.083924079579237 19.923393551337753 29.40772924765607 90-91 28.14667988107037 21.94534900184058 19.949030157157015 29.95894095993204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.5 8 1.5 9 3.0 10 4.0 11 5.5 12 10.0 13 16.5 14 24.5 15 34.0 16 42.0 17 52.5 18 77.0 19 100.0 20 139.0 21 206.0 22 277.0 23 375.0 24 508.0 25 664.0 26 870.5 27 1178.0 28 1511.5 29 1848.5 30 2295.5 31 2822.0 32 3409.0 33 4161.0 34 5227.0 35 6433.5 36 7727.5 37 9116.5 38 10684.0 39 12740.5 40 14869.0 41 16971.0 42 19159.5 43 21304.0 44 23441.5 45 25726.5 46 28185.5 47 30510.0 48 32488.0 49 34219.0 50 35862.5 51 37200.0 52 38009.5 53 38412.0 54 38545.0 55 38412.5 56 38251.5 57 37879.5 58 37159.5 59 36041.5 60 34738.5 61 33640.5 62 32309.0 63 30538.0 64 28566.5 65 26460.5 66 24128.5 67 21965.5 68 19836.0 69 17296.0 70 14798.0 71 12610.0 72 10557.0 73 8560.5 74 6715.0 75 5291.0 76 4093.5 77 3042.0 78 2351.5 79 1799.0 80 1277.5 81 898.5 82 643.0 83 422.0 84 286.0 85 201.5 86 128.0 87 87.5 88 65.0 89 41.5 90 22.0 91 13.5 92 8.5 93 3.5 94 2.5 95 3.0 96 2.0 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0015783575611218966 2 0.0063134302444875865 3 3.9458939028047416E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 11125.0 32-33 23680.0 34-35 24791.0 36-37 25994.0 38-39 27267.0 40-41 28055.0 42-43 27992.0 44-45 27332.0 46-47 26260.0 48-49 25334.0 50-51 24061.0 52-53 22802.0 54-55 21131.0 56-57 19542.0 58-59 17988.0 60-61 16670.0 62-63 15490.0 64-65 14248.0 66-67 12918.0 68-69 11883.0 70-71 10919.0 72-73 10050.0 74-75 9444.0 76-77 8364.0 78-79 7848.0 80-81 7215.0 82-83 6581.0 84-85 6092.0 86-87 5683.0 88-89 5319.0 90-91 4778.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.81874771061095 #Duplication Level Percentage of deduplicated Percentage of total 1 99.82087441075748 99.63994679059982 2 0.17666976863349507 0.35269910126637705 3 0.0024558206090094007 0.007354108133796848 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0017756522562621337 0.0 0.0 0.0 0.0 2 0.0017756522562621337 0.0 0.0 0.0 0.0 3 0.0017756522562621337 0.0 0.0 0.0 0.0 4 0.0017756522562621337 0.0 0.0 0.0 0.0 5 0.0017756522562621337 0.0 0.0 0.0 0.0 6 0.0019729469514023707 0.0 0.0 0.0 0.0 7 0.0027621257319633188 0.0 0.0 0.0 0.0 8 0.007497198415329008 0.0 0.0 0.0 0.0 9 0.007694493110469246 0.0 0.0 0.0 0.0 10-11 0.007694493110469246 0.0 0.0 0.0 9.864734757011854E-5 12-13 0.007694493110469246 0.0 0.0 0.0 1.9729469514023708E-4 14-15 0.007694493110469246 0.0 0.0 0.0 1.9729469514023708E-4 16-17 0.007694493110469246 0.0 0.0 0.0 1.9729469514023708E-4 18-19 0.007694493110469246 0.0 0.0 0.0 1.9729469514023708E-4 20-21 0.007694493110469246 0.0 0.0 0.0 1.9729469514023708E-4 22-23 0.00818772984831984 0.0 1.9729469514023708E-4 0.0 2.9594204271035564E-4 24-25 0.008582319238600313 0.0 1.9729469514023708E-4 0.0 3.9458939028047416E-4 26-27 0.008779613933740549 0.0 1.9729469514023708E-4 0.0 3.9458939028047416E-4 28-29 0.009075555976450905 0.0 1.9729469514023708E-4 0.0 3.9458939028047416E-4 30-31 0.009568792714301498 0.0 1.9729469514023708E-4 0.0 3.9458939028047416E-4 32-33 0.010357971494862447 0.0 1.9729469514023708E-4 0.0 3.9458939028047416E-4 34-35 0.01085120823271304 0.0 1.9729469514023708E-4 0.0 4.932367378505927E-4 36-37 0.010949855580283156 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 38-39 0.01154173966570387 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 40-41 0.011640387013273986 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 42-43 0.011640387013273986 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 44-45 0.011837681708414224 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 46-47 0.012133623751124579 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 48-49 0.012232271098694698 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 50-51 0.012232271098694698 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 52-53 0.012528213141405054 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 54-55 0.012626860488975173 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 56-57 0.012626860488975173 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 58-59 0.01282415518411541 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 60-61 0.013021449879255647 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 62-63 0.013120097226825765 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 64-65 0.013218744574395884 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 66-67 0.013218744574395884 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 68-69 0.013317391921966001 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 70-71 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 72-73 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 74-75 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 76-77 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 78-79 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 80 0.01341603926953612 0.0 1.9729469514023708E-4 0.0 5.918840854207112E-4 >>END_MODULE