##FastQC 0.11.9 >>Basic Statistics pass #Measure Value Filename AM-AL-10B.merged.rmdup.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 315477 Sequences flagged as poor quality 0 Sequence length 30-91 %GC 54 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 39.23116423701252 40.0 40.0 40.0 40.0 40.0 2 39.12964812014822 40.0 40.0 40.0 40.0 40.0 3 39.11996754121537 40.0 40.0 40.0 40.0 40.0 4 39.0573385698482 40.0 40.0 40.0 40.0 40.0 5 39.20623056514421 40.0 40.0 40.0 40.0 40.0 6 39.23934866884115 40.0 40.0 40.0 40.0 40.0 7 39.285184022924014 40.0 40.0 40.0 40.0 40.0 8 39.303378693216935 40.0 40.0 40.0 40.0 40.0 9 39.33462027342722 40.0 40.0 40.0 40.0 40.0 10-11 39.32705078341686 40.0 40.0 40.0 40.0 40.0 12-13 39.31958906671485 40.0 40.0 40.0 40.0 40.0 14-15 39.33860788583637 40.0 40.0 40.0 40.0 40.0 16-17 39.474795309959205 40.0 40.0 40.0 40.0 40.0 18-19 39.49206439772155 40.0 40.0 40.0 40.0 40.0 20-21 39.49559555847178 40.0 40.0 40.0 40.0 40.0 22-23 39.50008399978445 40.0 40.0 40.0 40.0 40.0 24-25 39.50488308180945 40.0 40.0 40.0 40.0 40.0 26-27 39.482897326904975 40.0 40.0 40.0 40.0 40.0 28-29 39.4798860138774 40.0 40.0 40.0 40.0 40.0 30-31 39.44520843176244 40.0 40.0 40.0 40.0 40.0 32-33 39.44255317203638 40.0 40.0 40.0 40.0 40.0 34-35 39.43907395332403 40.0 40.0 40.0 40.0 40.0 36-37 39.4245373370667 40.0 40.0 40.0 40.0 40.0 38-39 39.4124187136905 40.0 40.0 40.0 40.0 40.0 40-41 39.39745376836305 40.0 40.0 40.0 40.0 40.0 42-43 39.37854234406082 40.0 40.0 40.0 40.0 40.0 44-45 39.37041737668272 40.0 40.0 40.0 40.0 40.0 46-47 39.362901676484995 40.0 40.0 40.0 40.0 40.0 48-49 39.37178150126219 40.0 40.0 40.0 40.0 40.0 50-51 39.36041739119084 40.0 40.0 40.0 40.0 40.0 52-53 39.34017271421348 40.0 40.0 40.0 40.0 40.0 54-55 39.327521730668906 40.0 40.0 40.0 40.0 40.0 56-57 39.32824793595598 40.0 40.0 40.0 40.0 40.0 58-59 39.31408602701353 40.0 40.0 40.0 40.0 40.0 60-61 39.28256584061908 40.0 40.0 40.0 40.0 40.0 62-63 39.32519112348514 40.0 40.0 40.0 40.0 40.0 64-65 39.33675421579454 40.0 40.0 40.0 40.0 40.0 66-67 39.34165177223704 40.0 40.0 40.0 40.0 40.0 68-69 39.329680845439675 40.0 40.0 40.0 40.0 40.0 70-71 39.322282918761104 40.0 40.0 40.0 40.0 40.0 72-73 39.33307551673904 40.0 40.0 40.0 40.0 40.0 74-75 39.32062199982774 40.0 40.0 40.0 40.0 40.0 76-77 39.31877753950743 40.0 40.0 40.0 40.0 40.0 78-79 39.31087837179639 40.0 40.0 40.0 40.0 40.0 80-81 39.30503258773552 40.0 40.0 40.0 40.0 40.0 82-83 39.250892692546 40.0 40.0 40.0 40.0 40.0 84-85 39.28516701062008 40.0 40.0 40.0 40.0 40.0 86-87 39.26576812361603 40.0 40.0 40.0 40.0 40.0 88-89 39.36144689368692 40.0 40.0 40.0 40.0 40.0 90-91 39.374304679837465 40.0 40.0 40.0 40.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24940198923288648 1101 2 0.12410413909558571 1101 3 -0.016536109056495718 1101 4 0.03408688768070789 1101 5 0.2837330552685131 1101 6 0.13933133015739685 1101 7 -0.05247356474932019 1101 8 -0.12762541311688835 1101 9 -0.2659277483925848 1101 10-11 0.08732380392700634 1101 12-13 -0.021269818732605472 1101 14-15 -0.1641202902731962 1101 16-17 -0.04011905891049139 1101 18-19 -0.04769959343916952 1101 20-21 0.011104999375334046 1101 22-23 0.12373452920248695 1101 24-25 0.09480460606179264 1101 26-27 -0.07736955503411025 1101 28-29 0.04058286615301654 1101 30-31 0.0710223525949445 1101 32-33 0.03833334404637867 1101 34-35 -0.0061025625064345945 1101 36-37 -0.09068707310861157 1101 38-39 -0.06718613857924538 1101 40-41 0.09332550317086685 1101 42-43 0.008214413449906033 1101 44-45 -0.16842196795833075 1101 46-47 -0.022552867349816097 1101 48-49 -0.19197962853161954 1101 50-51 -0.15007423032241718 1101 52-53 -0.08527403403967071 1101 54-55 -0.01743573070795179 1101 56-57 -0.172021278503955 1101 58-59 0.058282703276148595 1101 60-61 -0.13451309958728785 1101 62-63 -0.08575402520747133 1101 64-65 -0.4032855222443672 1101 66-67 -0.40586776241240585 1101 68-69 -0.02167087454461125 1101 70-71 -0.09645854073655613 1101 72-73 -0.031040449658021885 1101 74-75 -0.09488463629754307 1101 76-77 -0.18450185809010833 1101 78-79 -0.3681449883051613 1101 80-81 -0.4193288737524128 1101 82-83 0.40692066252722725 1101 84-85 0.7659053266329678 1101 86-87 0.7444542396418257 1101 88-89 -0.2813652188842539 1101 90-91 0.5173524392274373 1102 1 0.20946343316529692 1102 2 0.11632124099010355 1102 3 0.39821043472230144 1102 4 0.22169569464435313 1102 5 0.15920668558084827 1102 6 -0.09989038003205764 1102 7 0.12612346546060849 1102 8 -0.02747180328586296 1102 9 -0.1192815630367221 1102 10-11 -0.03853385096290651 1102 12-13 -0.31794160572697194 1102 14-15 -0.1245913604216824 1102 16-17 0.02767407985038517 1102 18-19 -0.06019319302954784 1102 20-21 -0.0991868593036287 1102 22-23 0.037815430380156556 1102 24-25 -0.1601877134466534 1102 26-27 -0.006504219652647691 1102 28-29 0.05573903614789799 1102 30-31 -0.14956136476296678 1102 32-33 0.14313366918901238 1102 34-35 0.02798629417532794 1102 36-37 0.055682000760228334 1102 38-39 0.09350913136291439 1102 40-41 0.0674339928146992 1102 42-43 -0.13481608112909527 1102 44-45 -0.07888979775239591 1102 46-47 -0.04609585240968528 1102 48-49 0.19434869142367717 1102 50-51 -0.050351826674670974 1102 52-53 0.02175566584908495 1102 54-55 -0.2693965004287051 1102 56-57 -0.16993669868727324 1102 58-59 0.05671694171969932 1102 60-61 0.02134598660973097 1102 62-63 -0.023098984194398042 1102 64-65 -0.3463306201757632 1102 66-67 -0.04999195911437937 1102 68-69 -0.21823447981948618 1102 70-71 -0.26574684182301667 1102 72-73 -0.41124539687328365 1102 74-75 -0.25173940601268185 1102 76-77 -0.5040794836608882 1102 78-79 -0.5356727671460462 1102 80-81 -0.48306334522651895 1102 82-83 -1.160844905238342 1102 84-85 -0.7427153630222065 1102 86-87 -2.08977035928865 1102 88-89 0.07419033667130037 1102 90-91 0.5173524392274373 1103 1 0.2866434700601488 1103 2 -0.042682740519737195 1103 3 0.04307970017291041 1103 4 0.15569332520676937 1103 5 0.019205688422751166 1103 6 -0.14207491600928535 1103 7 0.09152682778275079 1103 8 -0.07519294090080564 1103 9 -0.12410590888914186 1103 10-11 -0.02431722548837456 1103 12-13 0.15498199059494056 1103 14-15 0.046630181991965 1103 16-17 0.08016258285491773 1103 18-19 -0.045216828050683944 1103 20-21 -0.034865413519348465 1103 22-23 -0.15983555003993644 1103 24-25 0.07390227294926177 1103 26-27 0.05527684716407322 1103 28-29 -0.0027134219655309266 1103 30-31 0.13040264257801226 1103 32-33 0.019421518511165914 1103 34-35 0.11021035088911901 1103 36-37 -0.18384014158293382 1103 38-39 0.10233418848776665 1103 40-41 -0.07821178950629104 1103 42-43 0.0711488899825099 1103 44-45 0.057740240804584175 1103 46-47 -0.11981374102687425 1103 48-49 0.14990810412290756 1103 50-51 0.03886556786572726 1103 52-53 0.13977816247680153 1103 54-55 0.11870602902931893 1103 56-57 -0.17888560225631522 1103 58-59 -0.1316298439854222 1103 60-61 0.026337645754473726 1103 62-63 -0.10476233362594911 1103 64-65 0.008794710475115153 1103 66-67 -0.48249507244497636 1103 68-69 0.14372229588145302 1103 70-71 0.20197985647473615 1103 72-73 0.15179220008516836 1103 74-75 0.039623850111105696 1103 76-77 0.05703162745022183 1103 78-79 0.3998567144693226 1103 80-81 -0.06558335264046633 1103 82-83 0.2575700131765757 1103 84-85 -0.3218139716126487 1103 86-87 0.01718151236909904 1103 88-89 0.07419033667130037 1103 90-91 0.5173524392274373 1104 1 0.2460603016806715 1104 2 0.3673789928967537 1104 3 -0.00734646828791341 1104 4 0.20167251842423184 1104 5 0.1552451688858909 1104 6 -0.07186332314252297 1104 7 -0.032256355058429165 1104 8 0.10662688429329847 1104 9 -0.1518091184844863 1104 10-11 0.06543575045999717 1104 12-13 -4.678137200784249E-4 1104 14-15 -0.08283374056559012 1104 16-17 -3.529770391494935E-4 1104 18-19 -0.05279566695629967 1104 20-21 -0.12306375584606144 1104 22-23 -9.104164950954896E-4 1104 24-25 -0.02716698959401498 1104 26-27 -0.0960830053265127 1104 28-29 0.06890366815802906 1104 30-31 0.013628868885668055 1104 32-33 0.11613581160510478 1104 34-35 -0.11608125910710498 1104 36-37 -0.01490742417647084 1104 38-39 -0.1054732994633838 1104 40-41 -0.06492614401979324 1104 42-43 -0.058200138127737944 1104 44-45 -0.18504167373800584 1104 46-47 -0.2358926165854669 1104 48-49 -0.0985988068840058 1104 50-51 -0.1500353928504694 1104 52-53 0.029174028937653418 1104 54-55 -0.00946053441992234 1104 56-57 0.1340169195472214 1104 58-59 -0.24459342151796193 1104 60-61 -0.07522960519161614 1104 62-63 -0.0895566665984191 1104 64-65 -0.04798628006970773 1104 66-67 -0.23681602231337706 1104 68-69 -0.11261623921733133 1104 70-71 -0.08196401339542803 1104 72-73 0.27162948688950905 1104 74-75 -0.007954131741584547 1104 76-77 0.4439124842105997 1104 78-79 0.24325597960065437 1104 80-81 0.07012250346822668 1104 82-83 0.3534212966791941 1104 84-85 0.575429136156778 1104 86-87 0.5222320174196042 1104 88-89 -0.4801956282409847 1104 90-91 -0.015980894105894095 1201 1 0.15416389399479158 1201 2 0.14728182440919113 1201 3 -0.06924068603292 1201 4 0.04321001913393019 1201 5 0.0720517484415737 1201 6 -0.04214501442574914 1201 7 0.012004783224170978 1201 8 0.2531420394952235 1201 9 -0.010233496581832924 1201 10-11 0.09049840710160595 1201 12-13 -0.1316412164826417 1201 14-15 -0.06863562391473721 1201 16-17 -0.07260480401759395 1201 18-19 -0.12617085249295457 1201 20-21 0.0030606165736628554 1201 22-23 -0.11072378486748136 1201 24-25 -0.026323456813848622 1201 26-27 -0.10985530014121991 1201 28-29 -0.00829985845786041 1201 30-31 -0.06342055078000186 1201 32-33 -0.004626801206256914 1201 34-35 0.018990126296131393 1201 36-37 -0.017783283295642605 1201 38-39 -0.06774296962523607 1201 40-41 -0.03557011504632612 1201 42-43 0.056074658930839405 1201 44-45 -0.10947514099449052 1201 46-47 -0.05781162969529419 1201 48-49 -0.0901582039137594 1201 50-51 -0.08758514868387124 1201 52-53 0.11240470928716206 1201 54-55 -0.04561134900247055 1201 56-57 -0.0529887673800431 1201 58-59 -0.3757713821894413 1201 60-61 -0.33379941446366246 1201 62-63 0.14974490080305713 1201 64-65 -0.11220181061325718 1201 66-67 0.25600967480002623 1201 68-69 -0.0014397411555790995 1201 70-71 -0.1596130910445126 1201 72-73 -0.13810380608462935 1201 74-75 -0.07522095749124702 1201 76-77 -0.19695741178543358 1201 78-79 -0.2936908943325207 1201 80-81 -0.361387487804798 1201 82-83 0.4613424312347121 1201 84-85 0.5553790108434953 1201 86-87 0.2615713567589424 1201 88-89 0.13693543471051584 1201 90-91 -1.082647560772564 1202 1 -0.05341305943851893 1202 2 0.1998561341845928 1202 3 -0.07652032373684392 1202 4 0.1738220450954202 1202 5 -0.018836443986067763 1202 6 0.06599975074846753 1202 7 0.04257159880413042 1202 8 -0.0268098382681643 1202 9 0.09199560511087412 1202 10-11 0.0652418956572518 1202 12-13 -0.039852647812672615 1202 14-15 -0.04067032096687484 1202 16-17 -0.10598768997249408 1202 18-19 -0.11521691344267992 1202 20-21 -0.1634017093855249 1202 22-23 -0.011475503245115704 1202 24-25 -0.12921538867643534 1202 26-27 -0.005116639134790546 1202 28-29 -0.1608465822377596 1202 30-31 -0.08567325382441737 1202 32-33 -0.14695164647704217 1202 34-35 -0.12801440850901713 1202 36-37 -0.26046828543664446 1202 38-39 -0.1021380689891771 1202 40-41 -0.03743841631467859 1202 42-43 -0.018340886346791763 1202 44-45 -0.12315358985208036 1202 46-47 0.08748193551620176 1202 48-49 -0.08936499944220344 1202 50-51 -0.1851728926199101 1202 52-53 -0.21574196904843035 1202 54-55 0.15684088106866767 1202 56-57 -0.22436917341685358 1202 58-59 0.0966793884951116 1202 60-61 -0.4803279178887152 1202 62-63 -0.09356669166107423 1202 64-65 0.3525619064448051 1202 66-67 0.1481496373802358 1202 68-69 -0.7582189488326989 1202 70-71 0.33824094836825935 1202 72-73 0.4626284770005924 1202 74-75 -0.323370878737272 1202 76-77 0.0268671345991649 1202 78-79 -0.04442399243404083 1202 80-81 0.16440249113396987 1202 82-83 0.5773467276901343 1202 84-85 0.4992386599663021 1202 86-87 -1.149318654131065 1202 88-89 0.6075236700046318 1202 90-91 -2.149314227439234 1203 1 0.4286963790535907 1203 2 0.2036363028382766 1203 3 0.2792032393144268 1203 4 0.30918488909449593 1203 5 0.11880792192232548 1203 6 0.07568503493214962 1203 7 0.21470720769460883 1203 8 0.17320358244894862 1203 9 0.16443019775483236 1203 10-11 0.17609701901600516 1203 12-13 0.03619435452104369 1203 14-15 -0.032226453116194875 1203 16-17 0.10311081063516525 1203 18-19 0.05044319944046549 1203 20-21 -0.05378800235978076 1203 22-23 0.0922069924117821 1203 24-25 0.15456940266869168 1203 26-27 0.14683197443057594 1203 28-29 0.021895121149483998 1203 30-31 0.07233332232696199 1203 32-33 -0.11397061223533456 1203 34-35 0.07094411741324791 1203 36-37 -0.036271840160964075 1203 38-39 0.013291366137188731 1203 40-41 0.07017805231641461 1203 42-43 0.0582101186278976 1203 44-45 0.14136677842217438 1203 46-47 -0.03177358874118852 1203 48-49 0.02973719335830083 1203 50-51 0.15199581816337115 1203 52-53 -0.026958144845742993 1203 54-55 0.1380241481561839 1203 56-57 0.08369521506188704 1203 58-59 0.1633982133489269 1203 60-61 0.43866028345242825 1203 62-63 0.23115468286472662 1203 64-65 0.2814163693381815 1203 66-67 0.32894383964777774 1203 68-69 0.14734837168416703 1203 70-71 0.4422155384037296 1203 72-73 -0.3135147548388346 1203 74-75 0.11138992970310824 1203 76-77 0.33672511404026295 1203 78-79 0.6624340617924318 1203 80-81 0.25525017772633873 1203 82-83 -1.1258524467164719 1203 84-85 0.7659053266329678 1203 86-87 0.7444542396418257 1203 88-89 0.6075236700046318 1203 90-91 0.5173524392274373 1204 1 0.06154017784166399 1204 2 -0.2366801746299103 1204 3 -0.0848834479920697 1204 4 -0.1795452318337709 1204 5 -0.1734098018743424 1204 6 -0.09222796022542212 1204 7 -0.3863671221722953 1204 8 -0.26544053916730803 1204 9 -0.0709697651993082 1204 10-11 -0.31585079924759896 1204 12-13 -0.13163396439086483 1204 14-15 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2401 62-63 -0.04719106380215976 2401 64-65 0.018415072583053416 2401 66-67 0.09738153730748422 2401 68-69 0.16640847085356114 2401 70-71 0.22091082712521626 2401 72-73 0.2200256739472266 2401 74-75 0.1756155229888492 2401 76-77 0.34001054671745834 2401 78-79 0.2009797151549222 2401 80-81 0.2672330269184897 2401 82-83 0.44517096943424406 2401 84-85 0.3149962357238749 2401 86-87 0.1437897878145833 2401 88-89 0.2596975830481085 2401 90-91 0.5173524392274373 2402 1 0.3147508106444761 2402 2 0.31881011468600917 2402 3 0.3270546796127505 2402 4 0.20143059168631794 2402 5 0.21948923125292907 2402 6 0.2612614526581325 2402 7 0.24281434594514906 2402 8 0.07515972376239688 2402 9 0.12182670669425022 2402 10-11 0.13553610834472352 2402 12-13 0.06612460646495322 2402 14-15 0.20100889026254976 2402 16-17 0.03979851915259047 2402 18-19 0.12793611971437002 2402 20-21 0.06743269450473122 2402 22-23 0.05279731932090925 2402 24-25 0.03067852767038204 2402 26-27 0.055329763399875276 2402 28-29 0.06819874369756462 2402 30-31 0.13099767954341957 2402 32-33 0.03827636771325871 2402 34-35 0.014184864657586616 2402 36-37 0.0593210605778296 2402 38-39 0.07947534820365121 2402 40-41 -0.06754683722925137 2402 42-43 0.06455789728040884 2402 44-45 -0.015482211538319746 2402 46-47 0.12535248687481726 2402 48-49 0.18496770315647382 2402 50-51 0.16710479772208942 2402 52-53 0.15335588230770725 2402 54-55 0.07643810740905366 2402 56-57 0.00332672799578404 2402 58-59 -0.039258745936713524 2402 60-61 0.1263958854727818 2402 62-63 0.014785905041037495 2402 64-65 0.25661157107649046 2402 66-67 0.23556562527740255 2402 68-69 0.22573242046162534 2402 70-71 -0.10492523782627217 2402 72-73 0.2763383311728802 2402 74-75 0.28270060968714716 2402 76-77 0.2210693258629206 2402 78-79 0.40390282321003923 2402 80-81 0.07367796872625121 2402 82-83 0.5956981297857027 2402 84-85 0.1129686370830072 2402 86-87 0.5844542396418291 2402 88-89 0.6075236700046318 2402 90-91 0.13640005827505775 2403 1 -0.2018651616952809 2403 2 -0.13834407182720554 2403 3 0.002640646391441237 2403 4 -0.07326850645434746 2403 5 0.08843577251359847 2403 6 0.10244136513183832 2403 7 0.17704486409113684 2403 8 0.05955779915106518 2403 9 0.04275129384577525 2403 10-11 0.14260494463187 2403 12-13 0.17954804513610867 2403 14-15 0.14879873044243652 2403 16-17 0.07257397310457492 2403 18-19 0.060430619097815 2403 20-21 0.06427691220825693 2403 22-23 0.011590326888786251 2403 24-25 0.11101122430230959 2403 26-27 0.10176415494705537 2403 28-29 0.05347498345191326 2403 30-31 0.05778587156185466 2403 32-33 0.0947115550132338 2403 34-35 0.09956431512893005 2403 36-37 4.887521396881311E-4 2403 38-39 0.10914866777614662 2403 40-41 0.06930005764228042 2403 42-43 0.03627877070054808 2403 44-45 0.11564033916206995 2403 46-47 0.21192401911324055 2403 48-49 0.10155560917545614 2403 50-51 0.07081233837099887 2403 52-53 0.17668864634234893 2403 54-55 0.17669635395426297 2403 56-57 0.16916170893588145 2403 58-59 0.27613054245996693 2403 60-61 0.10483311031028819 2403 62-63 0.2551590906277994 2403 64-65 0.20799875153228697 2403 66-67 0.28567416429980597 2403 68-69 0.09552057470236974 2403 70-71 0.31239249616691467 2403 72-73 0.16598688484918966 2403 74-75 -0.03143878133999323 2403 76-77 -0.15392933351073879 2403 78-79 0.20602380538218057 2403 80-81 0.2526180056026419 2403 82-83 0.4351457707523352 2403 84-85 0.32349495287247976 2403 86-87 0.4171815123690976 2403 88-89 0.6075236700046318 2403 90-91 0.5173524392274373 2404 1 -0.4233155425184023 2404 2 -0.3401652924802647 2404 3 -0.580968367730982 2404 4 -0.3969279978675999 2404 5 -0.24923890695560402 2404 6 -0.3486793428962969 2404 7 -0.1911728388556142 2404 8 -0.0921648225272449 2404 9 -0.254893323592448 2404 10-11 -0.0978347686571226 2404 12-13 0.1148599387075393 2404 14-15 0.03571793312558924 2404 16-17 -0.048707925738931124 2404 18-19 -0.02001268368740483 2404 20-21 0.020313659675977647 2404 22-23 -0.09571227886113576 2404 24-25 -0.12384592496464819 2404 26-27 -0.017129831525032557 2404 28-29 -0.029615572807834667 2404 30-31 0.027544365768186196 2404 32-33 0.05442712299991115 2404 34-35 0.045789427661176774 2404 36-37 0.06753392538587377 2404 38-39 0.07268133315624681 2404 40-41 0.10547150625422574 2404 42-43 -0.00573222424615949 2404 44-45 -0.07133657036831664 2404 46-47 -0.1219484486502509 2404 48-49 -0.03729705299022612 2404 50-51 0.028340646133059977 2404 52-53 -0.02555368502369504 2404 54-55 0.10871335070429211 2404 56-57 0.04465900696669678 2404 58-59 -0.01905098406822958 2404 60-61 0.24972899837509743 2404 62-63 0.30978003570409385 2404 64-65 0.041821728234829436 2404 66-67 0.18500735686608039 2404 68-69 0.16165435902291847 2404 70-71 0.1994550153037622 2404 72-73 0.07340194476887518 2404 74-75 0.10968957101123067 2404 76-77 0.45874102915962567 2404 78-79 0.4657127503170173 2404 80-81 -0.07205140957524492 2404 82-83 -0.4096763438672326 2404 84-85 0.02251817445158366 2404 86-87 -0.4440378238502376 2404 88-89 -0.31734405486309214 2404 90-91 0.5173524392274373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 30 13.0 31 195.0 32 1346.0 33 2475.0 34 3199.0 35 3999.0 36 5752.0 37 8892.0 38 23418.0 39 127378.0 40 138810.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.731745045581913 12.072233703990161 29.498281961226848 30.69773928920108 2 26.26300089710549 18.285736022747805 30.7770581913973 24.67420488874941 3 27.088127706245206 19.37941003062059 22.066794727933207 31.465667535200996 4 27.07487392107824 20.77615800834926 21.059538413259922 31.08942965731258 5 27.760502350409062 20.67979599146689 21.27033032519011 30.28937133293394 6 27.84513609549983 23.002310786523264 23.398853165206972 25.75369995276993 7 26.501456524564386 22.850160233551097 22.672968235402262 27.975415006482248 8 25.39424427137319 23.762429590746713 23.3303854163695 27.512940721510603 9 25.029399924558682 23.96593095534698 23.843893532650558 27.160775587443776 10-11 24.9454001401053 22.509406390957185 23.75498055325745 28.790212915680062 12-13 25.344002890860505 22.155656355296898 23.49695857384215 29.003382180000443 14-15 25.724696253609615 22.10715836653702 23.277608193307277 28.89053718654609 16-17 25.87288455259813 21.806978004735686 23.05207669655791 29.268060746108276 18-19 25.981291821590798 22.06198867112341 22.916884590635767 29.039834916650026 20-21 25.871616631323363 22.142343181911837 23.01324660751814 28.972793579246662 22-23 25.75053014958301 21.695400932556097 23.27111009677409 29.282958821086797 24-25 25.672077520706736 22.13584508537865 23.41359275002615 28.778484643888465 26-27 25.850537440130346 22.28783714819147 23.240679986179657 28.62094542549853 28-29 25.978122018403877 21.881785359947 23.306294912148907 28.83379770950022 30-31 26.140203505880116 22.07912004311028 23.272418930484672 28.508257520524932 32-33 26.121094475518614 22.12964804462846 23.481059367095057 28.26819811275787 34-35 26.084479347427703 21.740134898756448 23.634380249126703 28.54100550468915 36-37 26.22159006726524 22.116595549234006 23.657473256882096 28.004341126618666 38-39 26.208857864487005 22.076845056977508 23.759719984885546 27.95457709364994 40-41 26.114546955908413 21.780221576149597 23.850616731751796 28.2546147361902 42-43 26.288657380311363 21.952709820906005 23.872176050567717 27.88645674821491 44-45 26.267800430283785 22.156541337977668 23.77599631185329 27.79966191988526 46-47 26.435192095916992 21.679617638857987 23.888277093537095 27.996913171687925 48-49 26.371613152058625 22.037878575805365 23.87581998189741 27.7146882902386 50-51 26.54171579883738 21.961218339918766 23.739975313239135 27.757090548004715 52-53 26.7932449047599 21.63844383074725 23.668879296146347 27.899431968346505 54-55 26.71629481169367 21.93994196754798 23.649719880174523 27.69404334058383 56-57 26.850453352137638 22.003420986177883 23.50760669141085 27.63851897027363 58-59 27.242826298003724 21.719778674914192 23.32666670203341 27.710728325048674 60-61 26.971769327243898 22.143803686788612 23.219905818878818 27.66452116708867 62-63 27.41822460798054 22.062828886479625 23.01808710368353 27.50085940185631 64-65 27.692273623137055 21.772925570786366 22.518472588229216 28.016328217847363 66-67 27.644329982005182 21.921483169068726 22.495996566126824 27.93819028279927 68-69 27.532657772503498 21.82438508821539 22.533631247624257 28.109325891656855 70-71 27.515920898273837 21.86085972850679 22.2473604826546 28.37585889056477 72-73 27.76176411278365 21.910252304188464 21.996844714897453 28.331138868130434 74-75 28.092745153741056 21.829471206525707 21.99586055760724 28.081923082125996 76-77 28.405307825760772 21.962770197402353 21.51419952798487 28.117722448852 78-79 28.489424324966638 21.889910604924957 21.244584194073234 28.376080876035175 80-81 28.405537209963477 21.976075404028254 21.38780151770289 28.23058586830538 82-83 28.758769562871024 21.92390717754992 20.547760388559094 28.769562871019964 84-85 28.54627071823204 21.69889502762431 20.034530386740332 29.720303867403313 86-87 29.530233220918632 21.236878354628793 19.132665178359378 30.100223246093194 88-89 29.55951123849751 20.395233066827576 17.717604465228543 32.327651229446374 90-91 24.883387154646574 21.438823107283817 19.93182633656261 33.745963401507 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 0.5 9 0.0 10 1.5 11 4.5 12 5.5 13 4.5 14 10.0 15 24.5 16 43.0 17 61.0 18 86.0 19 124.5 20 174.0 21 235.5 22 308.5 23 414.5 24 557.5 25 663.5 26 779.5 27 1014.0 28 1285.5 29 1524.0 30 1817.0 31 2229.5 32 2653.0 33 3025.0 34 3518.5 35 4114.0 36 4834.0 37 5689.0 38 6699.0 39 7833.5 40 8870.0 41 9998.5 42 11302.0 43 12697.0 44 14117.5 45 15489.0 46 16801.0 47 18108.5 48 19404.0 49 20457.0 50 21221.5 51 22018.0 52 22747.0 53 23446.5 54 23906.0 55 23972.0 56 23948.0 57 23733.0 58 23475.0 59 23277.5 60 23042.5 61 22397.5 62 21347.0 63 20322.0 64 19276.5 65 17958.5 66 16331.5 67 14803.0 68 13368.0 69 11751.5 70 10108.0 71 8576.5 72 7156.5 73 5734.5 74 4471.5 75 3599.0 76 2852.5 77 2095.0 78 1555.0 79 1144.0 80 789.0 81 551.0 82 395.5 83 275.0 84 186.5 85 125.5 86 79.5 87 58.5 88 46.0 89 31.0 90 18.0 91 11.0 92 7.0 93 6.0 94 5.5 95 5.5 96 5.5 97 5.0 98 4.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.169803186920124E-4 2 0.005705645736456224 3 9.509409560760372E-4 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 30-31 7343.0 32-33 16074.0 34-35 17617.0 36-37 18315.0 38-39 18860.0 40-41 19105.0 42-43 18440.0 44-45 17832.0 46-47 17069.0 48-49 16037.0 50-51 14690.0 52-53 13880.0 54-55 12445.0 56-57 10923.0 58-59 10018.0 60-61 9104.0 62-63 8146.0 64-65 7225.0 66-67 6845.0 68-69 6299.0 70-71 5766.0 72-73 5231.0 74-75 5004.0 76-77 4721.0 78-79 4496.0 80-81 4436.0 82-83 4061.0 84-85 3988.0 86-87 3909.0 88-89 3854.0 90-91 3744.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.75811218104363 #Duplication Level Percentage of deduplicated Percentage of total 1 99.77169628861776 99.53036070852944 2 0.21487934346132356 0.42871915300807434 3 0.013051240154250885 0.03905901238228503 4 0.0 0.0 5 3.7312776665711654E-4 0.0018611260802021451 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012679212747680497 0.0 0.0 0.0 3.169803186920124E-4 2 0.0012679212747680497 0.0 0.0 0.0 3.169803186920124E-4 3 0.0012679212747680497 0.0 0.0 0.0 3.169803186920124E-4 4 0.0012679212747680497 0.0 0.0 0.0 3.169803186920124E-4 5 0.001584901593460062 0.0 0.0 0.0 3.169803186920124E-4 6 0.0019018819121520744 0.0 0.0 0.0 3.169803186920124E-4 7 0.0025358425495360993 0.0 0.0 0.0 3.169803186920124E-4 8 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 9 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 10-11 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 12-13 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 14-15 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 16-17 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 18-19 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 20-21 0.01172827179160446 0.0 0.0 0.0 3.169803186920124E-4 22-23 0.012045252110296471 0.0 0.0 0.0 3.169803186920124E-4 24-25 0.012045252110296471 0.0 0.0 0.0 3.169803186920124E-4 26-27 0.012203742269642479 0.0 0.0 0.0 4.7547047803801865E-4 28-29 0.012362232428988484 0.0 0.0 0.0 6.339606373840248E-4 30-31 0.01299619306637251 0.0 0.0 0.0 6.339606373840248E-4 32-33 0.013313173385064521 0.0 0.0 0.0 6.339606373840248E-4 34-35 0.01378864386310254 0.0 0.0 0.0 6.339606373840248E-4 36-37 0.014581094659832572 0.0 0.0 0.0 6.339606373840248E-4 38-39 0.014898074978524583 0.0 0.0 0.0 6.339606373840248E-4 40-41 0.015056565137870589 0.0 3.169803186920124E-4 0.0 6.339606373840248E-4 42-43 0.015373545456562603 0.0 3.169803186920124E-4 0.0 6.339606373840248E-4 44-45 0.01584901593460062 0.0 3.169803186920124E-4 0.0 0.0014264114341140557 46-47 0.01584901593460062 0.0 3.169803186920124E-4 0.0 0.001584901593460062 48-49 0.016165996253292632 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 50-51 0.016641466731330653 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 52-53 0.01679995689067666 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 54-55 0.016958447050022665 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 56-57 0.017275427368714676 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 58-59 0.017433917528060682 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 60-61 0.017433917528060682 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 62-63 0.017433917528060682 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 64-65 0.017433917528060682 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 66-67 0.017592407687406688 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 68-69 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 70-71 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 72-73 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 74-75 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 76-77 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 78-79 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 80 0.017750897846752694 0.0 3.169803186920124E-4 0.0 0.0019018819121520744 >>END_MODULE